SHROOM4 cDNA ORF clone, Bos taurus(cattle)

The following SHROOM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHROOM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa49993 XM_010800493.2
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa49993 XM_010821949.3
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa49994 XM_010821950.3
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa49994 XM_015470468.1
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa49995 XM_015470470.1
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49995 XM_010821952.3
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89376 XM_015461686.2
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89376 XM_015470469.1
Latest version!
Bos taurus shroom family member 4 (SHROOM4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa89376 XM_015461686.1 Bos taurus shroom family member 4 (SHROOM4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49993 XM_010821949.2 Bos taurus shroom family member 4 (SHROOM4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa49994 XM_010821950.2 Bos taurus shroom family member 4 (SHROOM4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa49995 XM_010821952.2 Bos taurus shroom family member 4 (SHROOM4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa49993
    Clone ID Related Accession (Same CDS sequence) XM_010821949.3 , XM_010800493.2 , XM_010821949.2
    Accession Version XM_010821949.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821949.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
    TGGGGCTTCA CCCTTAAGGG GGGTCTGGAA CATTGCGAAC CGCTCACGGT GTCTAAGATC
    GAAGATGGAG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT
    ATCAATGGCA CTCCATTGTA CGGCTCCCGC CAAGAGGCTC TCATCCTCAT CAAAGGCTCC
    TTCCGGACCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA
    TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT
    TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG
    TGGTGTCCAC TCTCCCGGCA CTGCAGCACT GAGAAAAGCA GCTCCATTGG CAGTATGGAG
    AGCCTGGAGC AACCAGGCCA AGCCACCTAT GAGGGCCATC TTTTGCCCAT TGACCAGAGC
    ACATACCCCA ACCAGCGTGA TTCAGCCTAT AGCTCCTTCT CAGCCAGCTC AAATGCCTCT
    GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG TGGACTGTAT CATGCAAGGC
    CCAGGGCCAA CTAAAGCCCC TAATAGTCGC CCTAATGTGG TCGAGACCTC AGGAGGTGGC
    TGGCGCACCA GTGGGGGTCA CCTGACTGCT AGCTCCCAGA TGTCATCCTG TCCTCAGGAG
    GTCTCCCACT CAGAGCCTGC CAAGGCTGCC AGGGGTCCCC CTCAACCTCC AGTGAGGCGA
    GACAGTCTTC AGGCCTCCAG AGCCCAACTC TTCAATGGAG AGCAGTGCAG GGCTTCTGAG
    CTTATAGATT CCTTGCAACA GAAGGAGAAA CCTAGCTTGG ACACCATGCT ATCCCCAAGG
    AACCCTAATA GGTTCTGCTG CCTCAGCGGG CAAGACCAAG TGACAAATGA AGGCCATCAG
    AACTGTGAGC TCAGCCAACC TCCTGAATCT AACCAGAAGG GCTCTGAGAA TCTACCGATG
    GAGGCCTCAG CCAAAGCTGT TGGAGTCCCA AAAGCATGTG ACAAAACTTC CAGCATAGGT
    TCCAGCCCAC TCAACCAGGC TTCAGCAGAG CTAGCTAAGA CTTCTTCTTT TGGCAGCTCT
    CCACACCTCA CAGGACCCAC AGGGCATCGC CATAGTGCCC CTGAACAGCT GCTGGTGTCC
    CACTTGCAGC GTGTGCACCT TGATACTAGG GACAGCAGGG GGACAGAGCT CCAAGCTGGG
    CAGGATGGGC AGGAGTGGAC CGTATCCCCT TTGCACATGC ACAGTAGCCA CACAAGCAAG
    AAAAGCCCAT GTGCCCCTGC AGGAGGAACC CAGGACCAGC CGAGCAAAGA CAGAAAGACC
    AAGCCAGTGG ATGATAGACC TCTGGGTTCA GGTCATCAGA GCCCAAGCAG TTCCCCACAT
    GGAGAGGCTG ACGGACACCA TCCAGAAAAA GGCTTCCAGG ATCCAAACAG AGCAAGCAGA
    GCTGATAGTG AACTGGTCAG CCAGCAGTCC TCTGGCTCCC TTGCTCAACA AACCAGGGAC
    TGTTCTTCAA CCTCTAAAGT AGCTGGCAGC ACAGAGGCAA CTGAGGAAGG GGACAGTGAG
    CCCAAGGAGT GTGGCCGGGT GGGCAGTAAG CGAAATGGAG GGTTCCGGGG CCGCTCGATC
    CAAAACCGGC GGAAGAGCGA ACGTTTTGCT ACCAATCTAC GTAATGAAAT TCAGAGGAGG
    AAGGCCCAGC TCCAGAAAGG CAAGAGTCCC TTGTCACAGC TGTGTTACAC CAAGGAGCCA
    GTGGAAGAGA CCGAGGAGAC CCTGGAAAGT CCTCCACTTC CTTCCTCTAA CTCAGCCCTT
    CTATCTTCAT ATAAAAAACC ACCAAGCCCC AGAGACAAGC TCTTCAACAA GAGCATGATA
    CTCAGGGCTA GGTCTTCAGA GTGCCTCAGT CAAGCCCCTG AGGGTCAAGA ATCTAGGATT
    GGCTTGGAGG GACGAGTGAG TCCTGGCCAG AGATCTGGCC AGTCCTCTTT GAGCCCAAAC
    ACCTGGTGGA AAGCATCTGA CCCATCCTCG TCAGACTCTG AAAAAACAAA CGTTCACCAT
    GGAGTCTGTG GAGGTCACTG GAGATGGTCA CCAGAGCACA ACCTGCAGCC ACATGTGGCA
    CTAGCCATGG AAGGCCCTTC CAACCCAGGT GACAAGGAAT TGAAAGCGTC TACTGTCCAA
    GCTGGGGAGG AAGCCATCCT CTTGCCCTTT GCAGACAGAA GAAAGTTCTT TGAAGAGAGT
    AGCAAATCCT TATCTACATC TCATTTGCCA GGTTTAACCA CTCATAACAA GACTTTTACC
    CAGAGACCAA AACCTATTGA CCAAAGCTTT CAGCCAATGA GCTCCAGCTA TAGAGAATTG
    AGGCGCCATC CCATAGACCA ATCATATCAC TCCACAGACC AACCCTATCA TGCCACAGAC
    CAATCATATC ATTCCATATC ACCCCTTCAG TCAGAAACTC CCACTTACAC AGAATGTTTT
    GCAAGCAAAG GTCTAGAACA ATCCATGTGC TGTAAGCCAC TACACTGTGG TGATTTTGAT
    TACCACAGGA CGTGCTCTTA CTCCTGCAAT GTCCAGGGAG CTGTAGTCCA TGACCCTTGC
    ATTTATTGTT CTGGGGAAAT CTGTCCTGCT TTGCGAAAGA GAAATATGAT TCCAAACTGC
    TACAATTGCC GGTGCCACCA TCACCAGTGC ATCCGGTGTT CAGCTTGCTG TCATAATCCC
    CAGCATGGTA CCCTCGAGGA CAGCAGCTTG AAACCTGGCA ACACTTGGAA ACCCAGGAAG
    CCGACAGTGC AGGAATTTCC TGGGGACAAA TGGAAACCAA TAACAGGAAA CAGGAAGACC
    AGCCAGTCAG GGAGGGAAAT GGCTCATTCC AAGGCTAGCT TTTCATGGGC AACCCCATTC
    CATCCTTGCC TTGAGAACCC AGCACTGGAC TTGTCAAACT ACCGAGCAGT CTCTTCTCTT
    GACCTCCTTG GAGACTTTAA GCACTCCTCC AAAAAAACAG AGGAAACCTC CGTTTATGAA
    GAGGAGAGCT CCCTAGCCTC CATGCCTCGC CCACTGCGCA GCCGTGCCTT CTCAGAAAGC
    CACATCACCC TGGATCCTCA GAGTTCCCAG GTCTGGGAGC AGCATAGGAA GGAGCTCTTC
    AACAAAGTTG ATGAGACCCA GCCAGATGCT CTGGGTGCCA GGAAGAAGGC CTTTCCTCCT
    CCTCGTCCTC CTCCTCCCAA CTGGGAGAAG TACAGGCTCT TCCGTGCAGC CCAGCAACAG
    CAACAGCAGC AAAAGCAGCA GCAGCAAGAG GAGGAAGAAG AGGAGGAGGA GGAAGAGGAA
    GAAGAAGAGG AGGAAGGGGT AGAGGAGGAG GAAGAAGAGG AGGAAGCGGG GGAGGAGGAG
    GAGCTGCCAC CCCAGTATTT CAGTTCAGAA ACCACTGGTT CTGGTGCCCT CCATCCTGAG
    GAGGTTCTGG AGCAGCCCCA GCTTCCGGCC TTTGGCCACC TGGAGGCCCC GAGGCAGGGC
    TCACAAAGCC TCCCAGCAGA GCCAGAATCC TTTGCTCTGC GTTCCAGTGA TTTCCTGCCT
    CCAGTAAGGG GCCACTTGGG ATCTCAAGCT GAAAAGGCTC AACCTCCTTG TTATTATGGC
    ATTGGTGGGC TTTGGAGGAC ATCAGAGCAG GAGACCACTG ATTCCAAACC AGAAGCTTTG
    ATGGCGGAAG AGTCCAAATC CAAACCAGCA TGGAGCCAGA GCTACTTCTT TCCTGAGGAG
    CAGTTCTCTT TCATGGGCTG GGGCTCAGAT AAGCAGCACC TCAACCCTTC AGGCTTCAGT
    GAACCCAAGA CAACAGGACA AGATTTTCAG CACTTCTCAC CTCCTCAGGG GGCCCCAGGG
    ATCCCTACCT CTTATTCAGC TTATTACAAT ATTTCTGTGG CCAAAGCAGA GCTGTTGAAC
    AAGTTGAAAG ACCAACCTGA GATGTTAGAG ATTGGCCTAG GAGAGGAGGA AATTGACCAC
    GAACTGGCTC AAAAAAAGGT ACAGCTTATT GACAGCATTG GCAGAAAACT CTCTGTCCTG
    CGGGAGGCCC AGCGAGGACT GCTGGAAGAC ATCACTGCCA ATTCTGCCCT TGGGGAGGAG
    GTGGAGGCCA ACCTAAAAGC CCTCTGCAAA TCCAATGAGT TTGAAAAATA CCGTTTATTT
    ATCGGGGACC TGGACAAAGT AGTCAACCTG TTGCTGTCAC TCTCTGGTCG GCTGGCACGG
    GTGGAGAATG CTCTCAACAG CATCGATTCA GAGGCCAACC AGGAGAAGTT GGTGCTGATA
    GAGAAGAAGC AGCAGCTGAC AGGGCAGTTG GCAGATGCCA AGGAGCTGAA GGAGCATGTG
    GACCGTCGGG AGAAGTTGGT GTTTGGCATG GTCTCCCGTT ACCTGCCTCA GGACCAGCTC
    CAAGATTACC AGCACTTTGT GAAGATGAAA TCTGCTCTCA TCATTGAACA GCGAGAGCTG
    GAAGAGAAGA TCAAGCTCGG GGAAGAACAG CTCAAATGTC TCAGGGAAAG CCTGCTCCTG
    GGGCCCAGTA ATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820251.1
    CDS317..4954
    Translation

    Target ORF information:

    RefSeq Version XM_010821949.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821949.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
    TGGGGCTTCA CCCTTAAGGG GGGTCTGGAA CATTGCGAAC CGCTCACGGT GTCTAAGATC
    GAAGATGGAG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT
    ATCAATGGCA CTCCATTGTA CGGCTCCCGC CAAGAGGCTC TCATCCTCAT CAAAGGCTCC
    TTCCGGACCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA
    TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT
    TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG
    TGGTGTCCAC TCTCCCGGCA CTGCAGCACT GAGAAAAGCA GCTCCATTGG CAGTATGGAG
    AGCCTGGAGC AACCAGGCCA AGCCACCTAT GAGGGCCATC TTTTGCCCAT TGACCAGAGC
    ACATACCCCA ACCAGCGTGA TTCAGCCTAT AGCTCCTTCT CAGCCAGCTC AAATGCCTCT
    GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG TGGACTGTAT CATGCAAGGC
    CCAGGGCCAA CTAAAGCCCC TAATAGTCGC CCTAATGTGG TCGAGACCTC AGGAGGTGGC
    TGGCGCACCA GTGGGGGTCA CCTGACTGCT AGCTCCCAGA TGTCATCCTG TCCTCAGGAG
    GTCTCCCACT CAGAGCCTGC CAAGGCTGCC AGGGGTCCCC CTCAACCTCC AGTGAGGCGA
    GACAGTCTTC AGGCCTCCAG AGCCCAACTC TTCAATGGAG AGCAGTGCAG GGCTTCTGAG
    CTTATAGATT CCTTGCAACA GAAGGAGAAA CCTAGCTTGG ACACCATGCT ATCCCCAAGG
    AACCCTAATA GGTTCTGCTG CCTCAGCGGG CAAGACCAAG TGACAAATGA AGGCCATCAG
    AACTGTGAGC TCAGCCAACC TCCTGAATCT AACCAGAAGG GCTCTGAGAA TCTACCGATG
    GAGGCCTCAG CCAAAGCTGT TGGAGTCCCA AAAGCATGTG ACAAAACTTC CAGCATAGGT
    TCCAGCCCAC TCAACCAGGC TTCAGCAGAG CTAGCTAAGA CTTCTTCTTT TGGCAGCTCT
    CCACACCTCA CAGGACCCAC AGGGCATCGC CATAGTGCCC CTGAACAGCT GCTGGTGTCC
    CACTTGCAGC GTGTGCACCT TGATACTAGG GACAGCAGGG GGACAGAGCT CCAAGCTGGG
    CAGGATGGGC AGGAGTGGAC CGTATCCCCT TTGCACATGC ACAGTAGCCA CACAAGCAAG
    AAAAGCCCAT GTGCCCCTGC AGGAGGAACC CAGGACCAGC CGAGCAAAGA CAGAAAGACC
    AAGCCAGTGG ATGATAGACC TCTGGGTTCA GGTCATCAGA GCCCAAGCAG TTCCCCACAT
    GGAGAGGCTG ACGGACACCA TCCAGAAAAA GGCTTCCAGG ATCCAAACAG AGCAAGCAGA
    GCTGATAGTG AACTGGTCAG CCAGCAGTCC TCTGGCTCCC TTGCTCAACA AACCAGGGAC
    TGTTCTTCAA CCTCTAAAGT AGCTGGCAGC ACAGAGGCAA CTGAGGAAGG GGACAGTGAG
    CCCAAGGAGT GTGGCCGGGT GGGCAGTAAG CGAAATGGAG GGTTCCGGGG CCGCTCGATC
    CAAAACCGGC GGAAGAGCGA ACGTTTTGCT ACCAATCTAC GTAATGAAAT TCAGAGGAGG
    AAGGCCCAGC TCCAGAAAGG CAAGAGTCCC TTGTCACAGC TGTGTTACAC CAAGGAGCCA
    GTGGAAGAGA CCGAGGAGAC CCTGGAAAGT CCTCCACTTC CTTCCTCTAA CTCAGCCCTT
    CTATCTTCAT ATAAAAAACC ACCAAGCCCC AGAGACAAGC TCTTCAACAA GAGCATGATA
    CTCAGGGCTA GGTCTTCAGA GTGCCTCAGT CAAGCCCCTG AGGGTCAAGA ATCTAGGATT
    GGCTTGGAGG GACGAGTGAG TCCTGGCCAG AGATCTGGCC AGTCCTCTTT GAGCCCAAAC
    ACCTGGTGGA AAGCATCTGA CCCATCCTCG TCAGACTCTG AAAAAACAAA CGTTCACCAT
    GGAGTCTGTG GAGGTCACTG GAGATGGTCA CCAGAGCACA ACCTGCAGCC ACATGTGGCA
    CTAGCCATGG AAGGCCCTTC CAACCCAGGT GACAAGGAAT TGAAAGCGTC TACTGTCCAA
    GCTGGGGAGG AAGCCATCCT CTTGCCCTTT GCAGACAGAA GAAAGTTCTT TGAAGAGAGT
    AGCAAATCCT TATCTACATC TCATTTGCCA GGTTTAACCA CTCATAACAA GACTTTTACC
    CAGAGACCAA AACCTATTGA CCAAAGCTTT CAGCCAATGA GCTCCAGCTA TAGAGAATTG
    AGGCGCCATC CCATAGACCA ATCATATCAC TCCACAGACC AACCCTATCA TGCCACAGAC
    CAATCATATC ATTCCATATC ACCCCTTCAG TCAGAAACTC CCACTTACAC AGAATGTTTT
    GCAAGCAAAG GTCTAGAACA ATCCATGTGC TGTAAGCCAC TACACTGTGG TGATTTTGAT
    TACCACAGGA CGTGCTCTTA CTCCTGCAAT GTCCAGGGAG CTGTAGTCCA TGACCCTTGC
    ATTTATTGTT CTGGGGAAAT CTGTCCTGCT TTGCGAAAGA GAAATATGAT TCCAAACTGC
    TACAATTGCC GGTGCCACCA TCACCAGTGC ATCCGGTGTT CAGCTTGCTG TCATAATCCC
    CAGCATGGTA CCCTCGAGGA CAGCAGCTTG AAACCTGGCA ACACTTGGAA ACCCAGGAAG
    CCGACAGTGC AGGAATTTCC TGGGGACAAA TGGAAACCAA TAACAGGAAA CAGGAAGACC
    AGCCAGTCAG GGAGGGAAAT GGCTCATTCC AAGGCTAGCT TTTCATGGGC AACCCCATTC
    CATCCTTGCC TTGAGAACCC AGCACTGGAC TTGTCAAACT ACCGAGCAGT CTCTTCTCTT
    GACCTCCTTG GAGACTTTAA GCACTCCTCC AAAAAAACAG AGGAAACCTC CGTTTATGAA
    GAGGAGAGCT CCCTAGCCTC CATGCCTCGC CCACTGCGCA GCCGTGCCTT CTCAGAAAGC
    CACATCACCC TGGATCCTCA GAGTTCCCAG GTCTGGGAGC AGCATAGGAA GGAGCTCTTC
    AACAAAGTTG ATGAGACCCA GCCAGATGCT CTGGGTGCCA GGAAGAAGGC CTTTCCTCCT
    CCTCGTCCTC CTCCTCCCAA CTGGGAGAAG TACAGGCTCT TCCGTGCAGC CCAGCAACAG
    CAACAGCAGC AAAAGCAGCA GCAGCAAGAG GAGGAAGAAG AGGAGGAGGA GGAAGAGGAA
    GAAGAAGAGG AGGAAGGGGT AGAGGAGGAG GAAGAAGAGG AGGAAGCGGG GGAGGAGGAG
    GAGCTGCCAC CCCAGTATTT CAGTTCAGAA ACCACTGGTT CTGGTGCCCT CCATCCTGAG
    GAGGTTCTGG AGCAGCCCCA GCTTCCGGCC TTTGGCCACC TGGAGGCCCC GAGGCAGGGC
    TCACAAAGCC TCCCAGCAGA GCCAGAATCC TTTGCTCTGC GTTCCAGTGA TTTCCTGCCT
    CCAGTAAGGG GCCACTTGGG ATCTCAAGCT GAAAAGGCTC AACCTCCTTG TTATTATGGC
    ATTGGTGGGC TTTGGAGGAC ATCAGAGCAG GAGACCACTG ATTCCAAACC AGAAGCTTTG
    ATGGCGGAAG AGTCCAAATC CAAACCAGCA TGGAGCCAGA GCTACTTCTT TCCTGAGGAG
    CAGTTCTCTT TCATGGGCTG GGGCTCAGAT AAGCAGCACC TCAACCCTTC AGGCTTCAGT
    GAACCCAAGA CAACAGGACA AGATTTTCAG CACTTCTCAC CTCCTCAGGG GGCCCCAGGG
    ATCCCTACCT CTTATTCAGC TTATTACAAT ATTTCTGTGG CCAAAGCAGA GCTGTTGAAC
    AAGTTGAAAG ACCAACCTGA GATGTTAGAG ATTGGCCTAG GAGAGGAGGA AATTGACCAC
    GAACTGGCTC AAAAAAAGGT ACAGCTTATT GACAGCATTG GCAGAAAACT CTCTGTCCTG
    CGGGAGGCCC AGCGAGGACT GCTGGAAGAC ATCACTGCCA ATTCTGCCCT TGGGGAGGAG
    GTGGAGGCCA ACCTAAAAGC CCTCTGCAAA TCCAATGAGT TTGAAAAATA CCGTTTATTT
    ATCGGGGACC TGGACAAAGT AGTCAACCTG TTGCTGTCAC TCTCTGGTCG GCTGGCACGG
    GTGGAGAATG CTCTCAACAG CATCGATTCA GAGGCCAACC AGGAGAAGTT GGTGCTGATA
    GAGAAGAAGC AGCAGCTGAC AGGGCAGTTG GCAGATGCCA AGGAGCTGAA GGAGCATGTG
    GACCGTCGGG AGAAGTTGGT GTTTGGCATG GTCTCCCGTT ACCTGCCTCA GGACCAGCTC
    CAAGATTACC AGCACTTTGT GAAGATGAAA TCTGCTCTCA TCATTGAACA GCGAGAGCTG
    GAAGAGAAGA TCAAGCTCGG GGAAGAACAG CTCAAATGTC TCAGGGAAAG CCTGCTCCTG
    GGGCCCAGTA ATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49993
    Clone ID Related Accession (Same CDS sequence) XM_010821949.3 , XM_010800493.2 , XM_010821949.2
    Accession Version XM_010800493.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
    TGGGGCTTCA CCCTTAAGGG GGGTCTGGAA CATTGCGAAC CGCTCACGGT GTCTAAGATC
    GAAGATGGAG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT
    ATCAATGGCA CTCCATTGTA CGGCTCCCGC CAAGAGGCTC TCATCCTCAT CAAAGGCTCC
    TTCCGGACCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA
    TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT
    TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG
    TGGTGTCCAC TCTCCCGGCA CTGCAGCACT GAGAAAAGCA GCTCCATTGG CAGTATGGAG
    AGCCTGGAGC AACCAGGCCA AGCCACCTAT GAGGGCCATC TTTTGCCCAT TGACCAGAGC
    ACATACCCCA ACCAGCGTGA TTCAGCCTAT AGCTCCTTCT CAGCCAGCTC AAATGCCTCT
    GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG TGGACTGTAT CATGCAAGGC
    CCAGGGCCAA CTAAAGCCCC TAATAGTCGC CCTAATGTGG TCGAGACCTC AGGAGGTGGC
    TGGCGCACCA GTGGGGGTCA CCTGACTGCT AGCTCCCAGA TGTCATCCTG TCCTCAGGAG
    GTCTCCCACT CAGAGCCTGC CAAGGCTGCC AGGGGTCCCC CTCAACCTCC AGTGAGGCGA
    GACAGTCTTC AGGCCTCCAG AGCCCAACTC TTCAATGGAG AGCAGTGCAG GGCTTCTGAG
    CTTATAGATT CCTTGCAACA GAAGGAGAAA CCTAGCTTGG ACACCATGCT ATCCCCAAGG
    AACCCTAATA GGTTCTGCTG CCTCAGCGGG CAAGACCAAG TGACAAATGA AGGCCATCAG
    AACTGTGAGC TCAGCCAACC TCCTGAATCT AACCAGAAGG GCTCTGAGAA TCTACCGATG
    GAGGCCTCAG CCAAAGCTGT TGGAGTCCCA AAAGCATGTG ACAAAACTTC CAGCATAGGT
    TCCAGCCCAC TCAACCAGGC TTCAGCAGAG CTAGCTAAGA CTTCTTCTTT TGGCAGCTCT
    CCACACCTCA CAGGACCCAC AGGGCATCGC CATAGTGCCC CTGAACAGCT GCTGGTGTCC
    CACTTGCAGC GTGTGCACCT TGATACTAGG GACAGCAGGG GGACAGAGCT CCAAGCTGGG
    CAGGATGGGC AGGAGTGGAC CGTATCCCCT TTGCACATGC ACAGTAGCCA CACAAGCAAG
    AAAAGCCCAT GTGCCCCTGC AGGAGGAACC CAGGACCAGC CGAGCAAAGA CAGAAAGACC
    AAGCCAGTGG ATGATAGACC TCTGGGTTCA GGTCATCAGA GCCCAAGCAG TTCCCCACAT
    GGAGAGGCTG ACGGACACCA TCCAGAAAAA GGCTTCCAGG ATCCAAACAG AGCAAGCAGA
    GCTGATAGTG AACTGGTCAG CCAGCAGTCC TCTGGCTCCC TTGCTCAACA AACCAGGGAC
    TGTTCTTCAA CCTCTAAAGT AGCTGGCAGC ACAGAGGCAA CTGAGGAAGG GGACAGTGAG
    CCCAAGGAGT GTGGCCGGGT GGGCAGTAAG CGAAATGGAG GGTTCCGGGG CCGCTCGATC
    CAAAACCGGC GGAAGAGCGA ACGTTTTGCT ACCAATCTAC GTAATGAAAT TCAGAGGAGG
    AAGGCCCAGC TCCAGAAAGG CAAGAGTCCC TTGTCACAGC TGTGTTACAC CAAGGAGCCA
    GTGGAAGAGA CCGAGGAGAC CCTGGAAAGT CCTCCACTTC CTTCCTCTAA CTCAGCCCTT
    CTATCTTCAT ATAAAAAACC ACCAAGCCCC AGAGACAAGC TCTTCAACAA GAGCATGATA
    CTCAGGGCTA GGTCTTCAGA GTGCCTCAGT CAAGCCCCTG AGGGTCAAGA ATCTAGGATT
    GGCTTGGAGG GACGAGTGAG TCCTGGCCAG AGATCTGGCC AGTCCTCTTT GAGCCCAAAC
    ACCTGGTGGA AAGCATCTGA CCCATCCTCG TCAGACTCTG AAAAAACAAA CGTTCACCAT
    GGAGTCTGTG GAGGTCACTG GAGATGGTCA CCAGAGCACA ACCTGCAGCC ACATGTGGCA
    CTAGCCATGG AAGGCCCTTC CAACCCAGGT GACAAGGAAT TGAAAGCGTC TACTGTCCAA
    GCTGGGGAGG AAGCCATCCT CTTGCCCTTT GCAGACAGAA GAAAGTTCTT TGAAGAGAGT
    AGCAAATCCT TATCTACATC TCATTTGCCA GGTTTAACCA CTCATAACAA GACTTTTACC
    CAGAGACCAA AACCTATTGA CCAAAGCTTT CAGCCAATGA GCTCCAGCTA TAGAGAATTG
    AGGCGCCATC CCATAGACCA ATCATATCAC TCCACAGACC AACCCTATCA TGCCACAGAC
    CAATCATATC ATTCCATATC ACCCCTTCAG TCAGAAACTC CCACTTACAC AGAATGTTTT
    GCAAGCAAAG GTCTAGAACA ATCCATGTGC TGTAAGCCAC TACACTGTGG TGATTTTGAT
    TACCACAGGA CGTGCTCTTA CTCCTGCAAT GTCCAGGGAG CTGTAGTCCA TGACCCTTGC
    ATTTATTGTT CTGGGGAAAT CTGTCCTGCT TTGCGAAAGA GAAATATGAT TCCAAACTGC
    TACAATTGCC GGTGCCACCA TCACCAGTGC ATCCGGTGTT CAGCTTGCTG TCATAATCCC
    CAGCATGGTA CCCTCGAGGA CAGCAGCTTG AAACCTGGCA ACACTTGGAA ACCCAGGAAG
    CCGACAGTGC AGGAATTTCC TGGGGACAAA TGGAAACCAA TAACAGGAAA CAGGAAGACC
    AGCCAGTCAG GGAGGGAAAT GGCTCATTCC AAGGCTAGCT TTTCATGGGC AACCCCATTC
    CATCCTTGCC TTGAGAACCC AGCACTGGAC TTGTCAAACT ACCGAGCAGT CTCTTCTCTT
    GACCTCCTTG GAGACTTTAA GCACTCCTCC AAAAAAACAG AGGAAACCTC CGTTTATGAA
    GAGGAGAGCT CCCTAGCCTC CATGCCTCGC CCACTGCGCA GCCGTGCCTT CTCAGAAAGC
    CACATCACCC TGGATCCTCA GAGTTCCCAG GTCTGGGAGC AGCATAGGAA GGAGCTCTTC
    AACAAAGTTG ATGAGACCCA GCCAGATGCT CTGGGTGCCA GGAAGAAGGC CTTTCCTCCT
    CCTCGTCCTC CTCCTCCCAA CTGGGAGAAG TACAGGCTCT TCCGTGCAGC CCAGCAACAG
    CAACAGCAGC AAAAGCAGCA GCAGCAAGAG GAGGAAGAAG AGGAGGAGGA GGAAGAGGAA
    GAAGAAGAGG AGGAAGGGGT AGAGGAGGAG GAAGAAGAGG AGGAAGCGGG GGAGGAGGAG
    GAGCTGCCAC CCCAGTATTT CAGTTCAGAA ACCACTGGTT CTGGTGCCCT CCATCCTGAG
    GAGGTTCTGG AGCAGCCCCA GCTTCCGGCC TTTGGCCACC TGGAGGCCCC GAGGCAGGGC
    TCACAAAGCC TCCCAGCAGA GCCAGAATCC TTTGCTCTGC GTTCCAGTGA TTTCCTGCCT
    CCAGTAAGGG GCCACTTGGG ATCTCAAGCT GAAAAGGCTC AACCTCCTTG TTATTATGGC
    ATTGGTGGGC TTTGGAGGAC ATCAGAGCAG GAGACCACTG ATTCCAAACC AGAAGCTTTG
    ATGGCGGAAG AGTCCAAATC CAAACCAGCA TGGAGCCAGA GCTACTTCTT TCCTGAGGAG
    CAGTTCTCTT TCATGGGCTG GGGCTCAGAT AAGCAGCACC TCAACCCTTC AGGCTTCAGT
    GAACCCAAGA CAACAGGACA AGATTTTCAG CACTTCTCAC CTCCTCAGGG GGCCCCAGGG
    ATCCCTACCT CTTATTCAGC TTATTACAAT ATTTCTGTGG CCAAAGCAGA GCTGTTGAAC
    AAGTTGAAAG ACCAACCTGA GATGTTAGAG ATTGGCCTAG GAGAGGAGGA AATTGACCAC
    GAACTGGCTC AAAAAAAGGT ACAGCTTATT GACAGCATTG GCAGAAAACT CTCTGTCCTG
    CGGGAGGCCC AGCGAGGACT GCTGGAAGAC ATCACTGCCA ATTCTGCCCT TGGGGAGGAG
    GTGGAGGCCA ACCTAAAAGC CCTCTGCAAA TCCAATGAGT TTGAAAAATA CCGTTTATTT
    ATCGGGGACC TGGACAAAGT AGTCAACCTG TTGCTGTCAC TCTCTGGTCG GCTGGCACGG
    GTGGAGAATG CTCTCAACAG CATCGATTCA GAGGCCAACC AGGAGAAGTT GGTGCTGATA
    GAGAAGAAGC AGCAGCTGAC AGGGCAGTTG GCAGATGCCA AGGAGCTGAA GGAGCATGTG
    GACCGTCGGG AGAAGTTGGT GTTTGGCATG GTCTCCCGTT ACCTGCCTCA GGACCAGCTC
    CAAGATTACC AGCACTTTGT GAAGATGAAA TCTGCTCTCA TCATTGAACA GCGAGAGCTG
    GAAGAGAAGA TCAAGCTCGG GGAAGAACAG CTCAAATGTC TCAGGGAAAG CCTGCTCCTG
    GGGCCCAGTA ATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010798795.2
    CDS143..4780
    Translation

    Target ORF information:

    RefSeq Version XM_010800493.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
    TGGGGCTTCA CCCTTAAGGG GGGTCTGGAA CATTGCGAAC CGCTCACGGT GTCTAAGATC
    GAAGATGGAG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT
    ATCAATGGCA CTCCATTGTA CGGCTCCCGC CAAGAGGCTC TCATCCTCAT CAAAGGCTCC
    TTCCGGACCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA
    TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT
    TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG
    TGGTGTCCAC TCTCCCGGCA CTGCAGCACT GAGAAAAGCA GCTCCATTGG CAGTATGGAG
    AGCCTGGAGC AACCAGGCCA AGCCACCTAT GAGGGCCATC TTTTGCCCAT TGACCAGAGC
    ACATACCCCA ACCAGCGTGA TTCAGCCTAT AGCTCCTTCT CAGCCAGCTC AAATGCCTCT
    GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG TGGACTGTAT CATGCAAGGC
    CCAGGGCCAA CTAAAGCCCC TAATAGTCGC CCTAATGTGG TCGAGACCTC AGGAGGTGGC
    TGGCGCACCA GTGGGGGTCA CCTGACTGCT AGCTCCCAGA TGTCATCCTG TCCTCAGGAG
    GTCTCCCACT CAGAGCCTGC CAAGGCTGCC AGGGGTCCCC CTCAACCTCC AGTGAGGCGA
    GACAGTCTTC AGGCCTCCAG AGCCCAACTC TTCAATGGAG AGCAGTGCAG GGCTTCTGAG
    CTTATAGATT CCTTGCAACA GAAGGAGAAA CCTAGCTTGG ACACCATGCT ATCCCCAAGG
    AACCCTAATA GGTTCTGCTG CCTCAGCGGG CAAGACCAAG TGACAAATGA AGGCCATCAG
    AACTGTGAGC TCAGCCAACC TCCTGAATCT AACCAGAAGG GCTCTGAGAA TCTACCGATG
    GAGGCCTCAG CCAAAGCTGT TGGAGTCCCA AAAGCATGTG ACAAAACTTC CAGCATAGGT
    TCCAGCCCAC TCAACCAGGC TTCAGCAGAG CTAGCTAAGA CTTCTTCTTT TGGCAGCTCT
    CCACACCTCA CAGGACCCAC AGGGCATCGC CATAGTGCCC CTGAACAGCT GCTGGTGTCC
    CACTTGCAGC GTGTGCACCT TGATACTAGG GACAGCAGGG GGACAGAGCT CCAAGCTGGG
    CAGGATGGGC AGGAGTGGAC CGTATCCCCT TTGCACATGC ACAGTAGCCA CACAAGCAAG
    AAAAGCCCAT GTGCCCCTGC AGGAGGAACC CAGGACCAGC CGAGCAAAGA CAGAAAGACC
    AAGCCAGTGG ATGATAGACC TCTGGGTTCA GGTCATCAGA GCCCAAGCAG TTCCCCACAT
    GGAGAGGCTG ACGGACACCA TCCAGAAAAA GGCTTCCAGG ATCCAAACAG AGCAAGCAGA
    GCTGATAGTG AACTGGTCAG CCAGCAGTCC TCTGGCTCCC TTGCTCAACA AACCAGGGAC
    TGTTCTTCAA CCTCTAAAGT AGCTGGCAGC ACAGAGGCAA CTGAGGAAGG GGACAGTGAG
    CCCAAGGAGT GTGGCCGGGT GGGCAGTAAG CGAAATGGAG GGTTCCGGGG CCGCTCGATC
    CAAAACCGGC GGAAGAGCGA ACGTTTTGCT ACCAATCTAC GTAATGAAAT TCAGAGGAGG
    AAGGCCCAGC TCCAGAAAGG CAAGAGTCCC TTGTCACAGC TGTGTTACAC CAAGGAGCCA
    GTGGAAGAGA CCGAGGAGAC CCTGGAAAGT CCTCCACTTC CTTCCTCTAA CTCAGCCCTT
    CTATCTTCAT ATAAAAAACC ACCAAGCCCC AGAGACAAGC TCTTCAACAA GAGCATGATA
    CTCAGGGCTA GGTCTTCAGA GTGCCTCAGT CAAGCCCCTG AGGGTCAAGA ATCTAGGATT
    GGCTTGGAGG GACGAGTGAG TCCTGGCCAG AGATCTGGCC AGTCCTCTTT GAGCCCAAAC
    ACCTGGTGGA AAGCATCTGA CCCATCCTCG TCAGACTCTG AAAAAACAAA CGTTCACCAT
    GGAGTCTGTG GAGGTCACTG GAGATGGTCA CCAGAGCACA ACCTGCAGCC ACATGTGGCA
    CTAGCCATGG AAGGCCCTTC CAACCCAGGT GACAAGGAAT TGAAAGCGTC TACTGTCCAA
    GCTGGGGAGG AAGCCATCCT CTTGCCCTTT GCAGACAGAA GAAAGTTCTT TGAAGAGAGT
    AGCAAATCCT TATCTACATC TCATTTGCCA GGTTTAACCA CTCATAACAA GACTTTTACC
    CAGAGACCAA AACCTATTGA CCAAAGCTTT CAGCCAATGA GCTCCAGCTA TAGAGAATTG
    AGGCGCCATC CCATAGACCA ATCATATCAC TCCACAGACC AACCCTATCA TGCCACAGAC
    CAATCATATC ATTCCATATC ACCCCTTCAG TCAGAAACTC CCACTTACAC AGAATGTTTT
    GCAAGCAAAG GTCTAGAACA ATCCATGTGC TGTAAGCCAC TACACTGTGG TGATTTTGAT
    TACCACAGGA CGTGCTCTTA CTCCTGCAAT GTCCAGGGAG CTGTAGTCCA TGACCCTTGC
    ATTTATTGTT CTGGGGAAAT CTGTCCTGCT TTGCGAAAGA GAAATATGAT TCCAAACTGC
    TACAATTGCC GGTGCCACCA TCACCAGTGC ATCCGGTGTT CAGCTTGCTG TCATAATCCC
    CAGCATGGTA CCCTCGAGGA CAGCAGCTTG AAACCTGGCA ACACTTGGAA ACCCAGGAAG
    CCGACAGTGC AGGAATTTCC TGGGGACAAA TGGAAACCAA TAACAGGAAA CAGGAAGACC
    AGCCAGTCAG GGAGGGAAAT GGCTCATTCC AAGGCTAGCT TTTCATGGGC AACCCCATTC
    CATCCTTGCC TTGAGAACCC AGCACTGGAC TTGTCAAACT ACCGAGCAGT CTCTTCTCTT
    GACCTCCTTG GAGACTTTAA GCACTCCTCC AAAAAAACAG AGGAAACCTC CGTTTATGAA
    GAGGAGAGCT CCCTAGCCTC CATGCCTCGC CCACTGCGCA GCCGTGCCTT CTCAGAAAGC
    CACATCACCC TGGATCCTCA GAGTTCCCAG GTCTGGGAGC AGCATAGGAA GGAGCTCTTC
    AACAAAGTTG ATGAGACCCA GCCAGATGCT CTGGGTGCCA GGAAGAAGGC CTTTCCTCCT
    CCTCGTCCTC CTCCTCCCAA CTGGGAGAAG TACAGGCTCT TCCGTGCAGC CCAGCAACAG
    CAACAGCAGC AAAAGCAGCA GCAGCAAGAG GAGGAAGAAG AGGAGGAGGA GGAAGAGGAA
    GAAGAAGAGG AGGAAGGGGT AGAGGAGGAG GAAGAAGAGG AGGAAGCGGG GGAGGAGGAG
    GAGCTGCCAC CCCAGTATTT CAGTTCAGAA ACCACTGGTT CTGGTGCCCT CCATCCTGAG
    GAGGTTCTGG AGCAGCCCCA GCTTCCGGCC TTTGGCCACC TGGAGGCCCC GAGGCAGGGC
    TCACAAAGCC TCCCAGCAGA GCCAGAATCC TTTGCTCTGC GTTCCAGTGA TTTCCTGCCT
    CCAGTAAGGG GCCACTTGGG ATCTCAAGCT GAAAAGGCTC AACCTCCTTG TTATTATGGC
    ATTGGTGGGC TTTGGAGGAC ATCAGAGCAG GAGACCACTG ATTCCAAACC AGAAGCTTTG
    ATGGCGGAAG AGTCCAAATC CAAACCAGCA TGGAGCCAGA GCTACTTCTT TCCTGAGGAG
    CAGTTCTCTT TCATGGGCTG GGGCTCAGAT AAGCAGCACC TCAACCCTTC AGGCTTCAGT
    GAACCCAAGA CAACAGGACA AGATTTTCAG CACTTCTCAC CTCCTCAGGG GGCCCCAGGG
    ATCCCTACCT CTTATTCAGC TTATTACAAT ATTTCTGTGG CCAAAGCAGA GCTGTTGAAC
    AAGTTGAAAG ACCAACCTGA GATGTTAGAG ATTGGCCTAG GAGAGGAGGA AATTGACCAC
    GAACTGGCTC AAAAAAAGGT ACAGCTTATT GACAGCATTG GCAGAAAACT CTCTGTCCTG
    CGGGAGGCCC AGCGAGGACT GCTGGAAGAC ATCACTGCCA ATTCTGCCCT TGGGGAGGAG
    GTGGAGGCCA ACCTAAAAGC CCTCTGCAAA TCCAATGAGT TTGAAAAATA CCGTTTATTT
    ATCGGGGACC TGGACAAAGT AGTCAACCTG TTGCTGTCAC TCTCTGGTCG GCTGGCACGG
    GTGGAGAATG CTCTCAACAG CATCGATTCA GAGGCCAACC AGGAGAAGTT GGTGCTGATA
    GAGAAGAAGC AGCAGCTGAC AGGGCAGTTG GCAGATGCCA AGGAGCTGAA GGAGCATGTG
    GACCGTCGGG AGAAGTTGGT GTTTGGCATG GTCTCCCGTT ACCTGCCTCA GGACCAGCTC
    CAAGATTACC AGCACTTTGT GAAGATGAAA TCTGCTCTCA TCATTGAACA GCGAGAGCTG
    GAAGAGAAGA TCAAGCTCGG GGAAGAACAG CTCAAATGTC TCAGGGAAAG CCTGCTCCTG
    GGGCCCAGTA ATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49993
    Clone ID Related Accession (Same CDS sequence) XM_010821949.3 , XM_010800493.2 , XM_010821949.2
    Accession Version XM_010821949.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104731.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
    TGGGGCTTCA CCCTTAAGGG GGGTCTGGAA CATTGCGAAC CGCTCACGGT GTCTAAGATC
    GAAGATGGAG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT
    ATCAATGGCA CTCCATTGTA CGGCTCCCGC CAAGAGGCTC TCATCCTCAT CAAAGGCTCC
    TTCCGGACCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA
    TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT
    TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG
    TGGTGTCCAC TCTCCCGGCA CTGCAGCACT GAGAAAAGCA GCTCCATTGG CAGTATGGAG
    AGCCTGGAGC AACCAGGCCA AGCCACCTAT GAGGGCCATC TTTTGCCCAT TGACCAGAGC
    ACATACCCCA ACCAGCGTGA TTCAGCCTAT AGCTCCTTCT CAGCCAGCTC AAATGCCTCT
    GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG TGGACTGTAT CATGCAAGGC
    CCAGGGCCAA CTAAAGCCCC TAATAGTCGC CCTAATGTGG TCGAGACCTC AGGAGGTGGC
    TGGCGCACCA GTGGGGGTCA CCTGACTGCT AGCTCCCAGA TGTCATCCTG TCCTCAGGAG
    GTCTCCCACT CAGAGCCTGC CAAGGCTGCC AGGGGTCCCC CTCAACCTCC AGTGAGGCGA
    GACAGTCTTC AGGCCTCCAG AGCCCAACTC TTCAATGGAG AGCAGTGCAG GGCTTCTGAG
    CTTATAGATT CCTTGCAACA GAAGGAGAAA CCTAGCTTGG ACACCATGCT ATCCCCAAGG
    AACCCTAATA GGTTCTGCTG CCTCAGCGGG CAAGACCAAG TGACAAATGA AGGCCATCAG
    AACTGTGAGC TCAGCCAACC TCCTGAATCT AACCAGAAGG GCTCTGAGAA TCTACCGATG
    GAGGCCTCAG CCAAAGCTGT TGGAGTCCCA AAAGCATGTG ACAAAACTTC CAGCATAGGT
    TCCAGCCCAC TCAACCAGGC TTCAGCAGAG CTAGCTAAGA CTTCTTCTTT TGGCAGCTCT
    CCACACCTCA CAGGACCCAC AGGGCATCGC CATAGTGCCC CTGAACAGCT GCTGGTGTCC
    CACTTGCAGC GTGTGCACCT TGATACTAGG GACAGCAGGG GGACAGAGCT CCAAGCTGGG
    CAGGATGGGC AGGAGTGGAC CGTATCCCCT TTGCACATGC ACAGTAGCCA CACAAGCAAG
    AAAAGCCCAT GTGCCCCTGC AGGAGGAACC CAGGACCAGC CGAGCAAAGA CAGAAAGACC
    AAGCCAGTGG ATGATAGACC TCTGGGTTCA GGTCATCAGA GCCCAAGCAG TTCCCCACAT
    GGAGAGGCTG ACGGACACCA TCCAGAAAAA GGCTTCCAGG ATCCAAACAG AGCAAGCAGA
    GCTGATAGTG AACTGGTCAG CCAGCAGTCC TCTGGCTCCC TTGCTCAACA AACCAGGGAC
    TGTTCTTCAA CCTCTAAAGT AGCTGGCAGC ACAGAGGCAA CTGAGGAAGG GGACAGTGAG
    CCCAAGGAGT GTGGCCGGGT GGGCAGTAAG CGAAATGGAG GGTTCCGGGG CCGCTCGATC
    CAAAACCGGC GGAAGAGCGA ACGTTTTGCT ACCAATCTAC GTAATGAAAT TCAGAGGAGG
    AAGGCCCAGC TCCAGAAAGG CAAGAGTCCC TTGTCACAGC TGTGTTACAC CAAGGAGCCA
    GTGGAAGAGA CCGAGGAGAC CCTGGAAAGT CCTCCACTTC CTTCCTCTAA CTCAGCCCTT
    CTATCTTCAT ATAAAAAACC ACCAAGCCCC AGAGACAAGC TCTTCAACAA GAGCATGATA
    CTCAGGGCTA GGTCTTCAGA GTGCCTCAGT CAAGCCCCTG AGGGTCAAGA ATCTAGGATT
    GGCTTGGAGG GACGAGTGAG TCCTGGCCAG AGATCTGGCC AGTCCTCTTT GAGCCCAAAC
    ACCTGGTGGA AAGCATCTGA CCCATCCTCG TCAGACTCTG AAAAAACAAA CGTTCACCAT
    GGAGTCTGTG GAGGTCACTG GAGATGGTCA CCAGAGCACA ACCTGCAGCC ACATGTGGCA
    CTAGCCATGG AAGGCCCTTC CAACCCAGGT GACAAGGAAT TGAAAGCGTC TACTGTCCAA
    GCTGGGGAGG AAGCCATCCT CTTGCCCTTT GCAGACAGAA GAAAGTTCTT TGAAGAGAGT
    AGCAAATCCT TATCTACATC TCATTTGCCA GGTTTAACCA CTCATAACAA GACTTTTACC
    CAGAGACCAA AACCTATTGA CCAAAGCTTT CAGCCAATGA GCTCCAGCTA TAGAGAATTG
    AGGCGCCATC CCATAGACCA ATCATATCAC TCCACAGACC AACCCTATCA TGCCACAGAC
    CAATCATATC ATTCCATATC ACCCCTTCAG TCAGAAACTC CCACTTACAC AGAATGTTTT
    GCAAGCAAAG GTCTAGAACA ATCCATGTGC TGTAAGCCAC TACACTGTGG TGATTTTGAT
    TACCACAGGA CGTGCTCTTA CTCCTGCAAT GTCCAGGGAG CTGTAGTCCA TGACCCTTGC
    ATTTATTGTT CTGGGGAAAT CTGTCCTGCT TTGCGAAAGA GAAATATGAT TCCAAACTGC
    TACAATTGCC GGTGCCACCA TCACCAGTGC ATCCGGTGTT CAGCTTGCTG TCATAATCCC
    CAGCATGGTA CCCTCGAGGA CAGCAGCTTG AAACCTGGCA ACACTTGGAA ACCCAGGAAG
    CCGACAGTGC AGGAATTTCC TGGGGACAAA TGGAAACCAA TAACAGGAAA CAGGAAGACC
    AGCCAGTCAG GGAGGGAAAT GGCTCATTCC AAGGCTAGCT TTTCATGGGC AACCCCATTC
    CATCCTTGCC TTGAGAACCC AGCACTGGAC TTGTCAAACT ACCGAGCAGT CTCTTCTCTT
    GACCTCCTTG GAGACTTTAA GCACTCCTCC AAAAAAACAG AGGAAACCTC CGTTTATGAA
    GAGGAGAGCT CCCTAGCCTC CATGCCTCGC CCACTGCGCA GCCGTGCCTT CTCAGAAAGC
    CACATCACCC TGGATCCTCA GAGTTCCCAG GTCTGGGAGC AGCATAGGAA GGAGCTCTTC
    AACAAAGTTG ATGAGACCCA GCCAGATGCT CTGGGTGCCA GGAAGAAGGC CTTTCCTCCT
    CCTCGTCCTC CTCCTCCCAA CTGGGAGAAG TACAGGCTCT TCCGTGCAGC CCAGCAACAG
    CAACAGCAGC AAAAGCAGCA GCAGCAAGAG GAGGAAGAAG AGGAGGAGGA GGAAGAGGAA
    GAAGAAGAGG AGGAAGGGGT AGAGGAGGAG GAAGAAGAGG AGGAAGCGGG GGAGGAGGAG
    GAGCTGCCAC CCCAGTATTT CAGTTCAGAA ACCACTGGTT CTGGTGCCCT CCATCCTGAG
    GAGGTTCTGG AGCAGCCCCA GCTTCCGGCC TTTGGCCACC TGGAGGCCCC GAGGCAGGGC
    TCACAAAGCC TCCCAGCAGA GCCAGAATCC TTTGCTCTGC GTTCCAGTGA TTTCCTGCCT
    CCAGTAAGGG GCCACTTGGG ATCTCAAGCT GAAAAGGCTC AACCTCCTTG TTATTATGGC
    ATTGGTGGGC TTTGGAGGAC ATCAGAGCAG GAGACCACTG ATTCCAAACC AGAAGCTTTG
    ATGGCGGAAG AGTCCAAATC CAAACCAGCA TGGAGCCAGA GCTACTTCTT TCCTGAGGAG
    CAGTTCTCTT TCATGGGCTG GGGCTCAGAT AAGCAGCACC TCAACCCTTC AGGCTTCAGT
    GAACCCAAGA CAACAGGACA AGATTTTCAG CACTTCTCAC CTCCTCAGGG GGCCCCAGGG
    ATCCCTACCT CTTATTCAGC TTATTACAAT ATTTCTGTGG CCAAAGCAGA GCTGTTGAAC
    AAGTTGAAAG ACCAACCTGA GATGTTAGAG ATTGGCCTAG GAGAGGAGGA AATTGACCAC
    GAACTGGCTC AAAAAAAGGT ACAGCTTATT GACAGCATTG GCAGAAAACT CTCTGTCCTG
    CGGGAGGCCC AGCGAGGACT GCTGGAAGAC ATCACTGCCA ATTCTGCCCT TGGGGAGGAG
    GTGGAGGCCA ACCTAAAAGC CCTCTGCAAA TCCAATGAGT TTGAAAAATA CCGTTTATTT
    ATCGGGGACC TGGACAAAGT AGTCAACCTG TTGCTGTCAC TCTCTGGTCG GCTGGCACGG
    GTGGAGAATG CTCTCAACAG CATCGATTCA GAGGCCAACC AGGAGAAGTT GGTGCTGATA
    GAGAAGAAGC AGCAGCTGAC AGGGCAGTTG GCAGATGCCA AGGAGCTGAA GGAGCATGTG
    GACCGTCGGG AGAAGTTGGT GTTTGGCATG GTCTCCCGTT ACCTGCCTCA GGACCAGCTC
    CAAGATTACC AGCACTTTGT GAAGATGAAA TCTGCTCTCA TCATTGAACA GCGAGAGCTG
    GAAGAGAAGA TCAAGCTCGG GGAAGAACAG CTCAAATGTC TCAGGGAAAG CCTGCTCCTG
    GGGCCCAGTA ATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820251.1
    CDS143..4780
    Translation

    Target ORF information:

    RefSeq Version XM_010821949.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821949.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
    TGGGGCTTCA CCCTTAAGGG GGGTCTGGAA CATTGCGAAC CGCTCACGGT GTCTAAGATC
    GAAGATGGAG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT
    ATCAATGGCA CTCCATTGTA CGGCTCCCGC CAAGAGGCTC TCATCCTCAT CAAAGGCTCC
    TTCCGGACCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA
    TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT
    TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG
    TGGTGTCCAC TCTCCCGGCA CTGCAGCACT GAGAAAAGCA GCTCCATTGG CAGTATGGAG
    AGCCTGGAGC AACCAGGCCA AGCCACCTAT GAGGGCCATC TTTTGCCCAT TGACCAGAGC
    ACATACCCCA ACCAGCGTGA TTCAGCCTAT AGCTCCTTCT CAGCCAGCTC AAATGCCTCT
    GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG TGGACTGTAT CATGCAAGGC
    CCAGGGCCAA CTAAAGCCCC TAATAGTCGC CCTAATGTGG TCGAGACCTC AGGAGGTGGC
    TGGCGCACCA GTGGGGGTCA CCTGACTGCT AGCTCCCAGA TGTCATCCTG TCCTCAGGAG
    GTCTCCCACT CAGAGCCTGC CAAGGCTGCC AGGGGTCCCC CTCAACCTCC AGTGAGGCGA
    GACAGTCTTC AGGCCTCCAG AGCCCAACTC TTCAATGGAG AGCAGTGCAG GGCTTCTGAG
    CTTATAGATT CCTTGCAACA GAAGGAGAAA CCTAGCTTGG ACACCATGCT ATCCCCAAGG
    AACCCTAATA GGTTCTGCTG CCTCAGCGGG CAAGACCAAG TGACAAATGA AGGCCATCAG
    AACTGTGAGC TCAGCCAACC TCCTGAATCT AACCAGAAGG GCTCTGAGAA TCTACCGATG
    GAGGCCTCAG CCAAAGCTGT TGGAGTCCCA AAAGCATGTG ACAAAACTTC CAGCATAGGT
    TCCAGCCCAC TCAACCAGGC TTCAGCAGAG CTAGCTAAGA CTTCTTCTTT TGGCAGCTCT
    CCACACCTCA CAGGACCCAC AGGGCATCGC CATAGTGCCC CTGAACAGCT GCTGGTGTCC
    CACTTGCAGC GTGTGCACCT TGATACTAGG GACAGCAGGG GGACAGAGCT CCAAGCTGGG
    CAGGATGGGC AGGAGTGGAC CGTATCCCCT TTGCACATGC ACAGTAGCCA CACAAGCAAG
    AAAAGCCCAT GTGCCCCTGC AGGAGGAACC CAGGACCAGC CGAGCAAAGA CAGAAAGACC
    AAGCCAGTGG ATGATAGACC TCTGGGTTCA GGTCATCAGA GCCCAAGCAG TTCCCCACAT
    GGAGAGGCTG ACGGACACCA TCCAGAAAAA GGCTTCCAGG ATCCAAACAG AGCAAGCAGA
    GCTGATAGTG AACTGGTCAG CCAGCAGTCC TCTGGCTCCC TTGCTCAACA AACCAGGGAC
    TGTTCTTCAA CCTCTAAAGT AGCTGGCAGC ACAGAGGCAA CTGAGGAAGG GGACAGTGAG
    CCCAAGGAGT GTGGCCGGGT GGGCAGTAAG CGAAATGGAG GGTTCCGGGG CCGCTCGATC
    CAAAACCGGC GGAAGAGCGA ACGTTTTGCT ACCAATCTAC GTAATGAAAT TCAGAGGAGG
    AAGGCCCAGC TCCAGAAAGG CAAGAGTCCC TTGTCACAGC TGTGTTACAC CAAGGAGCCA
    GTGGAAGAGA CCGAGGAGAC CCTGGAAAGT CCTCCACTTC CTTCCTCTAA CTCAGCCCTT
    CTATCTTCAT ATAAAAAACC ACCAAGCCCC AGAGACAAGC TCTTCAACAA GAGCATGATA
    CTCAGGGCTA GGTCTTCAGA GTGCCTCAGT CAAGCCCCTG AGGGTCAAGA ATCTAGGATT
    GGCTTGGAGG GACGAGTGAG TCCTGGCCAG AGATCTGGCC AGTCCTCTTT GAGCCCAAAC
    ACCTGGTGGA AAGCATCTGA CCCATCCTCG TCAGACTCTG AAAAAACAAA CGTTCACCAT
    GGAGTCTGTG GAGGTCACTG GAGATGGTCA CCAGAGCACA ACCTGCAGCC ACATGTGGCA
    CTAGCCATGG AAGGCCCTTC CAACCCAGGT GACAAGGAAT TGAAAGCGTC TACTGTCCAA
    GCTGGGGAGG AAGCCATCCT CTTGCCCTTT GCAGACAGAA GAAAGTTCTT TGAAGAGAGT
    AGCAAATCCT TATCTACATC TCATTTGCCA GGTTTAACCA CTCATAACAA GACTTTTACC
    CAGAGACCAA AACCTATTGA CCAAAGCTTT CAGCCAATGA GCTCCAGCTA TAGAGAATTG
    AGGCGCCATC CCATAGACCA ATCATATCAC TCCACAGACC AACCCTATCA TGCCACAGAC
    CAATCATATC ATTCCATATC ACCCCTTCAG TCAGAAACTC CCACTTACAC AGAATGTTTT
    GCAAGCAAAG GTCTAGAACA ATCCATGTGC TGTAAGCCAC TACACTGTGG TGATTTTGAT
    TACCACAGGA CGTGCTCTTA CTCCTGCAAT GTCCAGGGAG CTGTAGTCCA TGACCCTTGC
    ATTTATTGTT CTGGGGAAAT CTGTCCTGCT TTGCGAAAGA GAAATATGAT TCCAAACTGC
    TACAATTGCC GGTGCCACCA TCACCAGTGC ATCCGGTGTT CAGCTTGCTG TCATAATCCC
    CAGCATGGTA CCCTCGAGGA CAGCAGCTTG AAACCTGGCA ACACTTGGAA ACCCAGGAAG
    CCGACAGTGC AGGAATTTCC TGGGGACAAA TGGAAACCAA TAACAGGAAA CAGGAAGACC
    AGCCAGTCAG GGAGGGAAAT GGCTCATTCC AAGGCTAGCT TTTCATGGGC AACCCCATTC
    CATCCTTGCC TTGAGAACCC AGCACTGGAC TTGTCAAACT ACCGAGCAGT CTCTTCTCTT
    GACCTCCTTG GAGACTTTAA GCACTCCTCC AAAAAAACAG AGGAAACCTC CGTTTATGAA
    GAGGAGAGCT CCCTAGCCTC CATGCCTCGC CCACTGCGCA GCCGTGCCTT CTCAGAAAGC
    CACATCACCC TGGATCCTCA GAGTTCCCAG GTCTGGGAGC AGCATAGGAA GGAGCTCTTC
    AACAAAGTTG ATGAGACCCA GCCAGATGCT CTGGGTGCCA GGAAGAAGGC CTTTCCTCCT
    CCTCGTCCTC CTCCTCCCAA CTGGGAGAAG TACAGGCTCT TCCGTGCAGC CCAGCAACAG
    CAACAGCAGC AAAAGCAGCA GCAGCAAGAG GAGGAAGAAG AGGAGGAGGA GGAAGAGGAA
    GAAGAAGAGG AGGAAGGGGT AGAGGAGGAG GAAGAAGAGG AGGAAGCGGG GGAGGAGGAG
    GAGCTGCCAC CCCAGTATTT CAGTTCAGAA ACCACTGGTT CTGGTGCCCT CCATCCTGAG
    GAGGTTCTGG AGCAGCCCCA GCTTCCGGCC TTTGGCCACC TGGAGGCCCC GAGGCAGGGC
    TCACAAAGCC TCCCAGCAGA GCCAGAATCC TTTGCTCTGC GTTCCAGTGA TTTCCTGCCT
    CCAGTAAGGG GCCACTTGGG ATCTCAAGCT GAAAAGGCTC AACCTCCTTG TTATTATGGC
    ATTGGTGGGC TTTGGAGGAC ATCAGAGCAG GAGACCACTG ATTCCAAACC AGAAGCTTTG
    ATGGCGGAAG AGTCCAAATC CAAACCAGCA TGGAGCCAGA GCTACTTCTT TCCTGAGGAG
    CAGTTCTCTT TCATGGGCTG GGGCTCAGAT AAGCAGCACC TCAACCCTTC AGGCTTCAGT
    GAACCCAAGA CAACAGGACA AGATTTTCAG CACTTCTCAC CTCCTCAGGG GGCCCCAGGG
    ATCCCTACCT CTTATTCAGC TTATTACAAT ATTTCTGTGG CCAAAGCAGA GCTGTTGAAC
    AAGTTGAAAG ACCAACCTGA GATGTTAGAG ATTGGCCTAG GAGAGGAGGA AATTGACCAC
    GAACTGGCTC AAAAAAAGGT ACAGCTTATT GACAGCATTG GCAGAAAACT CTCTGTCCTG
    CGGGAGGCCC AGCGAGGACT GCTGGAAGAC ATCACTGCCA ATTCTGCCCT TGGGGAGGAG
    GTGGAGGCCA ACCTAAAAGC CCTCTGCAAA TCCAATGAGT TTGAAAAATA CCGTTTATTT
    ATCGGGGACC TGGACAAAGT AGTCAACCTG TTGCTGTCAC TCTCTGGTCG GCTGGCACGG
    GTGGAGAATG CTCTCAACAG CATCGATTCA GAGGCCAACC AGGAGAAGTT GGTGCTGATA
    GAGAAGAAGC AGCAGCTGAC AGGGCAGTTG GCAGATGCCA AGGAGCTGAA GGAGCATGTG
    GACCGTCGGG AGAAGTTGGT GTTTGGCATG GTCTCCCGTT ACCTGCCTCA GGACCAGCTC
    CAAGATTACC AGCACTTTGT GAAGATGAAA TCTGCTCTCA TCATTGAACA GCGAGAGCTG
    GAAGAGAAGA TCAAGCTCGG GGAAGAACAG CTCAAATGTC TCAGGGAAAG CCTGCTCCTG
    GGGCCCAGTA ATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49994
    Clone ID Related Accession (Same CDS sequence) XM_015470468.1 , XM_010821950.2 , XM_010821950.3
    Accession Version XM_015470468.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATCGAAG ATGGAGGCAA GGCAGCCTTG TCCCAGAAGA TGAGGACTGG TGATGAGCTG 
    GTGAATATCA ATGGCACTCC ATTGTACGGC TCCCGCCAAG AGGCTCTCAT CCTCATCAAA
    GGCTCCTTCC GGACCCTCAA GCTGATTGTC AGGAGGAGAA ACGCCCCTGT CAGTAGGCCG
    CACTCATGGC ATGTGGCCAA GCTGCTGGAG GGATGCCCTG AAGCGGCCAC CACCATGCAT
    TTCCCTTCTG AAGCCTTCAG CTTGTCCTGG CATTCCGGCT GCAACACAAG TGACGTGTGT
    GTGCAGTGGT GTCCACTCTC CCGGCACTGC AGCACTGAGA AAAGCAGCTC CATTGGCAGT
    ATGGAGAGCC TGGAGCAACC AGGCCAAGCC ACCTATGAGG GCCATCTTTT GCCCATTGAC
    CAGAGCACAT ACCCCAACCA GCGTGATTCA GCCTATAGCT CCTTCTCAGC CAGCTCAAAT
    GCCTCTGACT GTGCCCTTTC CCTCAGGCCA GAGGAGCCAG CCTCTGTGGA CTGTATCATG
    CAAGGCCCAG GGCCAACTAA AGCCCCTAAT AGTCGCCCTA ATGTGGTCGA GACCTCAGGA
    GGTGGCTGGC GCACCAGTGG GGGTCACCTG ACTGCTAGCT CCCAGATGTC ATCCTGTCCT
    CAGGAGGTCT CCCACTCAGA GCCTGCCAAG GCTGCCAGGG GTCCCCCTCA ACCTCCAGTG
    AGGCGAGACA GTCTTCAGGC CTCCAGAGCC CAACTCTTCA ATGGAGAGCA GTGCAGGGCT
    TCTGAGCTTA TAGATTCCTT GCAACAGAAG GAGAAACCTA GCTTGGACAC CATGCTATCC
    CCAAGGAACC CTAATAGGTT CTGCTGCCTC AGCGGGCAAG ACCAAGTGAC AAATGAAGGC
    CATCAGAACT GTGAGCTCAG CCAACCTCCT GAATCTAACC AGAAGGGCTC TGAGAATCTA
    CCGATGGAGG CCTCAGCCAA AGCTGTTGGA GTCCCAAAAG CATGTGACAA AACTTCCAGC
    ATAGGTTCCA GCCCACTCAA CCAGGCTTCA GCAGAGCTAG CTAAGACTTC TTCTTTTGGC
    AGCTCTCCAC ACCTCACAGG ACCCACAGGG CATCGCCATA GTGCCCCTGA ACAGCTGCTG
    GTGTCCCACT TGCAGCGTGT GCACCTTGAT ACTAGGGACA GCAGGGGGAC AGAGCTCCAA
    GCTGGGCAGG ATGGGCAGGA GTGGACCGTA TCCCCTTTGC ACATGCACAG TAGCCACACA
    AGCAAGAAAA GCCCATGTGC CCCTGCAGGA GGAACCCAGG ACCAGCCGAG CAAAGACAGA
    AAGACCAAGC CAGTGGATGA TAGACCTCTG GGTTCAGGTC ATCAGAGCCC AAGCAGTTCC
    CCACATGGAG AGGCTGACGG ACACCATCCA GAAAAAGGCT TCCAGGATCC AAACAGAGCA
    AGCAGAGCTG ATAGTGAACT GGTCAGCCAG CAGTCCTCTG GCTCCCTTGC TCAACAAACC
    AGGGACTGTT CTTCAACCTC TAAAGTAGCT GGCAGCACAG AGGCAACTGA GGAAGGGGAC
    AGTGAGCCCA AGGAGTGTGG CCGGGTGGGC AGTAAGCGAA ATGGAGGGTT CCGGGGCCGC
    TCGATCCAAA ACCGGCGGAA GAGCGAACGT TTTGCTACCA ATCTACGTAA TGAAATTCAG
    AGGAGGAAGG CCCAGCTCCA GAAAGGCAAG AGTCCCTTGT CACAGCTGTG TTACACCAAG
    GAGCCAGTGG AAGAGACCGA GGAGACCCTG GAAAGTCCTC CACTTCCTTC CTCTAACTCA
    GCCCTTCTAT CTTCATATAA AAAACCACCA AGCCCCAGAG ACAAGCTCTT CAACAAGAGC
    ATGATACTCA GGGCTAGGTC TTCAGAGTGC CTCAGTCAAG CCCCTGAGGG TCAAGAATCT
    AGGATTGGCT TGGAGGGACG AGTGAGTCCT GGCCAGAGAT CTGGCCAGTC CTCTTTGAGC
    CCAAACACCT GGTGGAAAGC ATCTGACCCA TCCTCGTCAG ACTCTGAAAA AACAAACGTT
    CACCATGGAG TCTGTGGAGG TCACTGGAGA TGGTCACCAG AGCACAACCT GCAGCCACAT
    GTGGCACTAG CCATGGAAGG CCCTTCCAAC CCAGGTGACA AGGAATTGAA AGCGTCTACT
    GTCCAAGCTG GGGAGGAAGC CATCCTCTTG CCCTTTGCAG ACAGAAGAAA GTTCTTTGAA
    GAGAGTAGCA AATCCTTATC TACATCTCAT TTGCCAGGTT TAACCACTCA TAACAAGACT
    TTTACCCAGA GACCAAAACC TATTGACCAA AGCTTTCAGC CAATGAGCTC CAGCTATAGA
    GAATTGAGGC GCCATCCCAT AGACCAATCA TATCACTCCA CAGACCAACC CTATCATGCC
    ACAGACCAAT CATATCATTC CATATCACCC CTTCAGTCAG AAACTCCCAC TTACACAGAA
    TGTTTTGCAA GCAAAGGTCT AGAACAATCC ATGTGCTGTA AGCCACTACA CTGTGGTGAT
    TTTGATTACC ACAGGACGTG CTCTTACTCC TGCAATGTCC AGGGAGCTGT AGTCCATGAC
    CCTTGCATTT ATTGTTCTGG GGAAATCTGT CCTGCTTTGC GAAAGAGAAA TATGATTCCA
    AACTGCTACA ATTGCCGGTG CCACCATCAC CAGTGCATCC GGTGTTCAGC TTGCTGTCAT
    AATCCCCAGC ATGGTACCCT CGAGGACAGC AGCTTGAAAC CTGGCAACAC TTGGAAACCC
    AGGAAGCCGA CAGTGCAGGA ATTTCCTGGG GACAAATGGA AACCAATAAC AGGAAACAGG
    AAGACCAGCC AGTCAGGGAG GGAAATGGCT CATTCCAAGG CTAGCTTTTC ATGGGCAACC
    CCATTCCATC CTTGCCTTGA GAACCCAGCA CTGGACTTGT CAAACTACCG AGCAGTCTCT
    TCTCTTGACC TCCTTGGAGA CTTTAAGCAC TCCTCCAAAA AAACAGAGGA AACCTCCGTT
    TATGAAGAGG AGAGCTCCCT AGCCTCCATG CCTCGCCCAC TGCGCAGCCG TGCCTTCTCA
    GAAAGCCACA TCACCCTGGA TCCTCAGAGT TCCCAGGTCT GGGAGCAGCA TAGGAAGGAG
    CTCTTCAACA AAGTTGATGA GACCCAGCCA GATGCTCTGG GTGCCAGGAA GAAGGCCTTT
    CCTCCTCCTC GTCCTCCTCC TCCCAACTGG GAGAAGTACA GGCTCTTCCG TGCAGCCCAG
    CAACAGCAAC AGCAGCAAAA GCAGCAGCAG CAAGAGGAGG AAGAAGAGGA GGAGGAGGAA
    GAGGAAGAAG AAGAGGAGGA AGGGGTAGAG GAGGAGGAAG AAGAGGAGGA AGCGGGGGAG
    GAGGAGGAGC TGCCACCCCA GTATTTCAGT TCAGAAACCA CTGGTTCTGG TGCCCTCCAT
    CCTGAGGAGG TTCTGGAGCA GCCCCAGCTT CCGGCCTTTG GCCACCTGGA GGCCCCGAGG
    CAGGGCTCAC AAAGCCTCCC AGCAGAGCCA GAATCCTTTG CTCTGCGTTC CAGTGATTTC
    CTGCCTCCAG TAAGGGGCCA CTTGGGATCT CAAGCTGAAA AGGCTCAACC TCCTTGTTAT
    TATGGCATTG GTGGGCTTTG GAGGACATCA GAGCAGGAGA CCACTGATTC CAAACCAGAA
    GCTTTGATGG CGGAAGAGTC CAAATCCAAA CCAGCATGGA GCCAGAGCTA CTTCTTTCCT
    GAGGAGCAGT TCTCTTTCAT GGGCTGGGGC TCAGATAAGC AGCACCTCAA CCCTTCAGGC
    TTCAGTGAAC CCAAGACAAC AGGACAAGAT TTTCAGCACT TCTCACCTCC TCAGGGGGCC
    CCAGGGATCC CTACCTCTTA TTCAGCTTAT TACAATATTT CTGTGGCCAA AGCAGAGCTG
    TTGAACAAGT TGAAAGACCA ACCTGAGATG TTAGAGATTG GCCTAGGAGA GGAGGAAATT
    GACCACGAAC TGGCTCAAAA AAAGGTACAG CTTATTGACA GCATTGGCAG AAAACTCTCT
    GTCCTGCGGG AGGCCCAGCG AGGACTGCTG GAAGACATCA CTGCCAATTC TGCCCTTGGG
    GAGGAGGTGG AGGCCAACCT AAAAGCCCTC TGCAAATCCA ATGAGTTTGA AAAATACCGT
    TTATTTATCG GGGACCTGGA CAAAGTAGTC AACCTGTTGC TGTCACTCTC TGGTCGGCTG
    GCACGGGTGG AGAATGCTCT CAACAGCATC GATTCAGAGG CCAACCAGGA GAAGTTGGTG
    CTGATAGAGA AGAAGCAGCA GCTGACAGGG CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG
    CATGTGGACC GTCGGGAGAA GTTGGTGTTT GGCATGGTCT CCCGTTACCT GCCTCAGGAC
    CAGCTCCAAG ATTACCAGCA CTTTGTGAAG ATGAAATCTG CTCTCATCAT TGAACAGCGA
    GAGCTGGAAG AGAAGATCAA GCTCGGGGAA GAACAGCTCA AATGTCTCAG GGAAAGCCTG
    CTCCTGGGGC CCAGTAATTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325954.1
    CDS54..4577
    Translation

    Target ORF information:

    RefSeq Version XM_015470468.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATCGAAG ATGGAGGCAA GGCAGCCTTG TCCCAGAAGA TGAGGACTGG TGATGAGCTG 
    GTGAATATCA ATGGCACTCC ATTGTACGGC TCCCGCCAAG AGGCTCTCAT CCTCATCAAA
    GGCTCCTTCC GGACCCTCAA GCTGATTGTC AGGAGGAGAA ACGCCCCTGT CAGTAGGCCG
    CACTCATGGC ATGTGGCCAA GCTGCTGGAG GGATGCCCTG AAGCGGCCAC CACCATGCAT
    TTCCCTTCTG AAGCCTTCAG CTTGTCCTGG CATTCCGGCT GCAACACAAG TGACGTGTGT
    GTGCAGTGGT GTCCACTCTC CCGGCACTGC AGCACTGAGA AAAGCAGCTC CATTGGCAGT
    ATGGAGAGCC TGGAGCAACC AGGCCAAGCC ACCTATGAGG GCCATCTTTT GCCCATTGAC
    CAGAGCACAT ACCCCAACCA GCGTGATTCA GCCTATAGCT CCTTCTCAGC CAGCTCAAAT
    GCCTCTGACT GTGCCCTTTC CCTCAGGCCA GAGGAGCCAG CCTCTGTGGA CTGTATCATG
    CAAGGCCCAG GGCCAACTAA AGCCCCTAAT AGTCGCCCTA ATGTGGTCGA GACCTCAGGA
    GGTGGCTGGC GCACCAGTGG GGGTCACCTG ACTGCTAGCT CCCAGATGTC ATCCTGTCCT
    CAGGAGGTCT CCCACTCAGA GCCTGCCAAG GCTGCCAGGG GTCCCCCTCA ACCTCCAGTG
    AGGCGAGACA GTCTTCAGGC CTCCAGAGCC CAACTCTTCA ATGGAGAGCA GTGCAGGGCT
    TCTGAGCTTA TAGATTCCTT GCAACAGAAG GAGAAACCTA GCTTGGACAC CATGCTATCC
    CCAAGGAACC CTAATAGGTT CTGCTGCCTC AGCGGGCAAG ACCAAGTGAC AAATGAAGGC
    CATCAGAACT GTGAGCTCAG CCAACCTCCT GAATCTAACC AGAAGGGCTC TGAGAATCTA
    CCGATGGAGG CCTCAGCCAA AGCTGTTGGA GTCCCAAAAG CATGTGACAA AACTTCCAGC
    ATAGGTTCCA GCCCACTCAA CCAGGCTTCA GCAGAGCTAG CTAAGACTTC TTCTTTTGGC
    AGCTCTCCAC ACCTCACAGG ACCCACAGGG CATCGCCATA GTGCCCCTGA ACAGCTGCTG
    GTGTCCCACT TGCAGCGTGT GCACCTTGAT ACTAGGGACA GCAGGGGGAC AGAGCTCCAA
    GCTGGGCAGG ATGGGCAGGA GTGGACCGTA TCCCCTTTGC ACATGCACAG TAGCCACACA
    AGCAAGAAAA GCCCATGTGC CCCTGCAGGA GGAACCCAGG ACCAGCCGAG CAAAGACAGA
    AAGACCAAGC CAGTGGATGA TAGACCTCTG GGTTCAGGTC ATCAGAGCCC AAGCAGTTCC
    CCACATGGAG AGGCTGACGG ACACCATCCA GAAAAAGGCT TCCAGGATCC AAACAGAGCA
    AGCAGAGCTG ATAGTGAACT GGTCAGCCAG CAGTCCTCTG GCTCCCTTGC TCAACAAACC
    AGGGACTGTT CTTCAACCTC TAAAGTAGCT GGCAGCACAG AGGCAACTGA GGAAGGGGAC
    AGTGAGCCCA AGGAGTGTGG CCGGGTGGGC AGTAAGCGAA ATGGAGGGTT CCGGGGCCGC
    TCGATCCAAA ACCGGCGGAA GAGCGAACGT TTTGCTACCA ATCTACGTAA TGAAATTCAG
    AGGAGGAAGG CCCAGCTCCA GAAAGGCAAG AGTCCCTTGT CACAGCTGTG TTACACCAAG
    GAGCCAGTGG AAGAGACCGA GGAGACCCTG GAAAGTCCTC CACTTCCTTC CTCTAACTCA
    GCCCTTCTAT CTTCATATAA AAAACCACCA AGCCCCAGAG ACAAGCTCTT CAACAAGAGC
    ATGATACTCA GGGCTAGGTC TTCAGAGTGC CTCAGTCAAG CCCCTGAGGG TCAAGAATCT
    AGGATTGGCT TGGAGGGACG AGTGAGTCCT GGCCAGAGAT CTGGCCAGTC CTCTTTGAGC
    CCAAACACCT GGTGGAAAGC ATCTGACCCA TCCTCGTCAG ACTCTGAAAA AACAAACGTT
    CACCATGGAG TCTGTGGAGG TCACTGGAGA TGGTCACCAG AGCACAACCT GCAGCCACAT
    GTGGCACTAG CCATGGAAGG CCCTTCCAAC CCAGGTGACA AGGAATTGAA AGCGTCTACT
    GTCCAAGCTG GGGAGGAAGC CATCCTCTTG CCCTTTGCAG ACAGAAGAAA GTTCTTTGAA
    GAGAGTAGCA AATCCTTATC TACATCTCAT TTGCCAGGTT TAACCACTCA TAACAAGACT
    TTTACCCAGA GACCAAAACC TATTGACCAA AGCTTTCAGC CAATGAGCTC CAGCTATAGA
    GAATTGAGGC GCCATCCCAT AGACCAATCA TATCACTCCA CAGACCAACC CTATCATGCC
    ACAGACCAAT CATATCATTC CATATCACCC CTTCAGTCAG AAACTCCCAC TTACACAGAA
    TGTTTTGCAA GCAAAGGTCT AGAACAATCC ATGTGCTGTA AGCCACTACA CTGTGGTGAT
    TTTGATTACC ACAGGACGTG CTCTTACTCC TGCAATGTCC AGGGAGCTGT AGTCCATGAC
    CCTTGCATTT ATTGTTCTGG GGAAATCTGT CCTGCTTTGC GAAAGAGAAA TATGATTCCA
    AACTGCTACA ATTGCCGGTG CCACCATCAC CAGTGCATCC GGTGTTCAGC TTGCTGTCAT
    AATCCCCAGC ATGGTACCCT CGAGGACAGC AGCTTGAAAC CTGGCAACAC TTGGAAACCC
    AGGAAGCCGA CAGTGCAGGA ATTTCCTGGG GACAAATGGA AACCAATAAC AGGAAACAGG
    AAGACCAGCC AGTCAGGGAG GGAAATGGCT CATTCCAAGG CTAGCTTTTC ATGGGCAACC
    CCATTCCATC CTTGCCTTGA GAACCCAGCA CTGGACTTGT CAAACTACCG AGCAGTCTCT
    TCTCTTGACC TCCTTGGAGA CTTTAAGCAC TCCTCCAAAA AAACAGAGGA AACCTCCGTT
    TATGAAGAGG AGAGCTCCCT AGCCTCCATG CCTCGCCCAC TGCGCAGCCG TGCCTTCTCA
    GAAAGCCACA TCACCCTGGA TCCTCAGAGT TCCCAGGTCT GGGAGCAGCA TAGGAAGGAG
    CTCTTCAACA AAGTTGATGA GACCCAGCCA GATGCTCTGG GTGCCAGGAA GAAGGCCTTT
    CCTCCTCCTC GTCCTCCTCC TCCCAACTGG GAGAAGTACA GGCTCTTCCG TGCAGCCCAG
    CAACAGCAAC AGCAGCAAAA GCAGCAGCAG CAAGAGGAGG AAGAAGAGGA GGAGGAGGAA
    GAGGAAGAAG AAGAGGAGGA AGGGGTAGAG GAGGAGGAAG AAGAGGAGGA AGCGGGGGAG
    GAGGAGGAGC TGCCACCCCA GTATTTCAGT TCAGAAACCA CTGGTTCTGG TGCCCTCCAT
    CCTGAGGAGG TTCTGGAGCA GCCCCAGCTT CCGGCCTTTG GCCACCTGGA GGCCCCGAGG
    CAGGGCTCAC AAAGCCTCCC AGCAGAGCCA GAATCCTTTG CTCTGCGTTC CAGTGATTTC
    CTGCCTCCAG TAAGGGGCCA CTTGGGATCT CAAGCTGAAA AGGCTCAACC TCCTTGTTAT
    TATGGCATTG GTGGGCTTTG GAGGACATCA GAGCAGGAGA CCACTGATTC CAAACCAGAA
    GCTTTGATGG CGGAAGAGTC CAAATCCAAA CCAGCATGGA GCCAGAGCTA CTTCTTTCCT
    GAGGAGCAGT TCTCTTTCAT GGGCTGGGGC TCAGATAAGC AGCACCTCAA CCCTTCAGGC
    TTCAGTGAAC CCAAGACAAC AGGACAAGAT TTTCAGCACT TCTCACCTCC TCAGGGGGCC
    CCAGGGATCC CTACCTCTTA TTCAGCTTAT TACAATATTT CTGTGGCCAA AGCAGAGCTG
    TTGAACAAGT TGAAAGACCA ACCTGAGATG TTAGAGATTG GCCTAGGAGA GGAGGAAATT
    GACCACGAAC TGGCTCAAAA AAAGGTACAG CTTATTGACA GCATTGGCAG AAAACTCTCT
    GTCCTGCGGG AGGCCCAGCG AGGACTGCTG GAAGACATCA CTGCCAATTC TGCCCTTGGG
    GAGGAGGTGG AGGCCAACCT AAAAGCCCTC TGCAAATCCA ATGAGTTTGA AAAATACCGT
    TTATTTATCG GGGACCTGGA CAAAGTAGTC AACCTGTTGC TGTCACTCTC TGGTCGGCTG
    GCACGGGTGG AGAATGCTCT CAACAGCATC GATTCAGAGG CCAACCAGGA GAAGTTGGTG
    CTGATAGAGA AGAAGCAGCA GCTGACAGGG CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG
    CATGTGGACC GTCGGGAGAA GTTGGTGTTT GGCATGGTCT CCCGTTACCT GCCTCAGGAC
    CAGCTCCAAG ATTACCAGCA CTTTGTGAAG ATGAAATCTG CTCTCATCAT TGAACAGCGA
    GAGCTGGAAG AGAAGATCAA GCTCGGGGAA GAACAGCTCA AATGTCTCAG GGAAAGCCTG
    CTCCTGGGGC CCAGTAATTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49994
    Clone ID Related Accession (Same CDS sequence) XM_015470468.1 , XM_010821950.2 , XM_010821950.3
    Accession Version XM_010821950.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104731.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATCGAAG ATGGAGGCAA GGCAGCCTTG TCCCAGAAGA TGAGGACTGG TGATGAGCTG 
    GTGAATATCA ATGGCACTCC ATTGTACGGC TCCCGCCAAG AGGCTCTCAT CCTCATCAAA
    GGCTCCTTCC GGACCCTCAA GCTGATTGTC AGGAGGAGAA ACGCCCCTGT CAGTAGGCCG
    CACTCATGGC ATGTGGCCAA GCTGCTGGAG GGATGCCCTG AAGCGGCCAC CACCATGCAT
    TTCCCTTCTG AAGCCTTCAG CTTGTCCTGG CATTCCGGCT GCAACACAAG TGACGTGTGT
    GTGCAGTGGT GTCCACTCTC CCGGCACTGC AGCACTGAGA AAAGCAGCTC CATTGGCAGT
    ATGGAGAGCC TGGAGCAACC AGGCCAAGCC ACCTATGAGG GCCATCTTTT GCCCATTGAC
    CAGAGCACAT ACCCCAACCA GCGTGATTCA GCCTATAGCT CCTTCTCAGC CAGCTCAAAT
    GCCTCTGACT GTGCCCTTTC CCTCAGGCCA GAGGAGCCAG CCTCTGTGGA CTGTATCATG
    CAAGGCCCAG GGCCAACTAA AGCCCCTAAT AGTCGCCCTA ATGTGGTCGA GACCTCAGGA
    GGTGGCTGGC GCACCAGTGG GGGTCACCTG ACTGCTAGCT CCCAGATGTC ATCCTGTCCT
    CAGGAGGTCT CCCACTCAGA GCCTGCCAAG GCTGCCAGGG GTCCCCCTCA ACCTCCAGTG
    AGGCGAGACA GTCTTCAGGC CTCCAGAGCC CAACTCTTCA ATGGAGAGCA GTGCAGGGCT
    TCTGAGCTTA TAGATTCCTT GCAACAGAAG GAGAAACCTA GCTTGGACAC CATGCTATCC
    CCAAGGAACC CTAATAGGTT CTGCTGCCTC AGCGGGCAAG ACCAAGTGAC AAATGAAGGC
    CATCAGAACT GTGAGCTCAG CCAACCTCCT GAATCTAACC AGAAGGGCTC TGAGAATCTA
    CCGATGGAGG CCTCAGCCAA AGCTGTTGGA GTCCCAAAAG CATGTGACAA AACTTCCAGC
    ATAGGTTCCA GCCCACTCAA CCAGGCTTCA GCAGAGCTAG CTAAGACTTC TTCTTTTGGC
    AGCTCTCCAC ACCTCACAGG ACCCACAGGG CATCGCCATA GTGCCCCTGA ACAGCTGCTG
    GTGTCCCACT TGCAGCGTGT GCACCTTGAT ACTAGGGACA GCAGGGGGAC AGAGCTCCAA
    GCTGGGCAGG ATGGGCAGGA GTGGACCGTA TCCCCTTTGC ACATGCACAG TAGCCACACA
    AGCAAGAAAA GCCCATGTGC CCCTGCAGGA GGAACCCAGG ACCAGCCGAG CAAAGACAGA
    AAGACCAAGC CAGTGGATGA TAGACCTCTG GGTTCAGGTC ATCAGAGCCC AAGCAGTTCC
    CCACATGGAG AGGCTGACGG ACACCATCCA GAAAAAGGCT TCCAGGATCC AAACAGAGCA
    AGCAGAGCTG ATAGTGAACT GGTCAGCCAG CAGTCCTCTG GCTCCCTTGC TCAACAAACC
    AGGGACTGTT CTTCAACCTC TAAAGTAGCT GGCAGCACAG AGGCAACTGA GGAAGGGGAC
    AGTGAGCCCA AGGAGTGTGG CCGGGTGGGC AGTAAGCGAA ATGGAGGGTT CCGGGGCCGC
    TCGATCCAAA ACCGGCGGAA GAGCGAACGT TTTGCTACCA ATCTACGTAA TGAAATTCAG
    AGGAGGAAGG CCCAGCTCCA GAAAGGCAAG AGTCCCTTGT CACAGCTGTG TTACACCAAG
    GAGCCAGTGG AAGAGACCGA GGAGACCCTG GAAAGTCCTC CACTTCCTTC CTCTAACTCA
    GCCCTTCTAT CTTCATATAA AAAACCACCA AGCCCCAGAG ACAAGCTCTT CAACAAGAGC
    ATGATACTCA GGGCTAGGTC TTCAGAGTGC CTCAGTCAAG CCCCTGAGGG TCAAGAATCT
    AGGATTGGCT TGGAGGGACG AGTGAGTCCT GGCCAGAGAT CTGGCCAGTC CTCTTTGAGC
    CCAAACACCT GGTGGAAAGC ATCTGACCCA TCCTCGTCAG ACTCTGAAAA AACAAACGTT
    CACCATGGAG TCTGTGGAGG TCACTGGAGA TGGTCACCAG AGCACAACCT GCAGCCACAT
    GTGGCACTAG CCATGGAAGG CCCTTCCAAC CCAGGTGACA AGGAATTGAA AGCGTCTACT
    GTCCAAGCTG GGGAGGAAGC CATCCTCTTG CCCTTTGCAG ACAGAAGAAA GTTCTTTGAA
    GAGAGTAGCA AATCCTTATC TACATCTCAT TTGCCAGGTT TAACCACTCA TAACAAGACT
    TTTACCCAGA GACCAAAACC TATTGACCAA AGCTTTCAGC CAATGAGCTC CAGCTATAGA
    GAATTGAGGC GCCATCCCAT AGACCAATCA TATCACTCCA CAGACCAACC CTATCATGCC
    ACAGACCAAT CATATCATTC CATATCACCC CTTCAGTCAG AAACTCCCAC TTACACAGAA
    TGTTTTGCAA GCAAAGGTCT AGAACAATCC ATGTGCTGTA AGCCACTACA CTGTGGTGAT
    TTTGATTACC ACAGGACGTG CTCTTACTCC TGCAATGTCC AGGGAGCTGT AGTCCATGAC
    CCTTGCATTT ATTGTTCTGG GGAAATCTGT CCTGCTTTGC GAAAGAGAAA TATGATTCCA
    AACTGCTACA ATTGCCGGTG CCACCATCAC CAGTGCATCC GGTGTTCAGC TTGCTGTCAT
    AATCCCCAGC ATGGTACCCT CGAGGACAGC AGCTTGAAAC CTGGCAACAC TTGGAAACCC
    AGGAAGCCGA CAGTGCAGGA ATTTCCTGGG GACAAATGGA AACCAATAAC AGGAAACAGG
    AAGACCAGCC AGTCAGGGAG GGAAATGGCT CATTCCAAGG CTAGCTTTTC ATGGGCAACC
    CCATTCCATC CTTGCCTTGA GAACCCAGCA CTGGACTTGT CAAACTACCG AGCAGTCTCT
    TCTCTTGACC TCCTTGGAGA CTTTAAGCAC TCCTCCAAAA AAACAGAGGA AACCTCCGTT
    TATGAAGAGG AGAGCTCCCT AGCCTCCATG CCTCGCCCAC TGCGCAGCCG TGCCTTCTCA
    GAAAGCCACA TCACCCTGGA TCCTCAGAGT TCCCAGGTCT GGGAGCAGCA TAGGAAGGAG
    CTCTTCAACA AAGTTGATGA GACCCAGCCA GATGCTCTGG GTGCCAGGAA GAAGGCCTTT
    CCTCCTCCTC GTCCTCCTCC TCCCAACTGG GAGAAGTACA GGCTCTTCCG TGCAGCCCAG
    CAACAGCAAC AGCAGCAAAA GCAGCAGCAG CAAGAGGAGG AAGAAGAGGA GGAGGAGGAA
    GAGGAAGAAG AAGAGGAGGA AGGGGTAGAG GAGGAGGAAG AAGAGGAGGA AGCGGGGGAG
    GAGGAGGAGC TGCCACCCCA GTATTTCAGT TCAGAAACCA CTGGTTCTGG TGCCCTCCAT
    CCTGAGGAGG TTCTGGAGCA GCCCCAGCTT CCGGCCTTTG GCCACCTGGA GGCCCCGAGG
    CAGGGCTCAC AAAGCCTCCC AGCAGAGCCA GAATCCTTTG CTCTGCGTTC CAGTGATTTC
    CTGCCTCCAG TAAGGGGCCA CTTGGGATCT CAAGCTGAAA AGGCTCAACC TCCTTGTTAT
    TATGGCATTG GTGGGCTTTG GAGGACATCA GAGCAGGAGA CCACTGATTC CAAACCAGAA
    GCTTTGATGG CGGAAGAGTC CAAATCCAAA CCAGCATGGA GCCAGAGCTA CTTCTTTCCT
    GAGGAGCAGT TCTCTTTCAT GGGCTGGGGC TCAGATAAGC AGCACCTCAA CCCTTCAGGC
    TTCAGTGAAC CCAAGACAAC AGGACAAGAT TTTCAGCACT TCTCACCTCC TCAGGGGGCC
    CCAGGGATCC CTACCTCTTA TTCAGCTTAT TACAATATTT CTGTGGCCAA AGCAGAGCTG
    TTGAACAAGT TGAAAGACCA ACCTGAGATG TTAGAGATTG GCCTAGGAGA GGAGGAAATT
    GACCACGAAC TGGCTCAAAA AAAGGTACAG CTTATTGACA GCATTGGCAG AAAACTCTCT
    GTCCTGCGGG AGGCCCAGCG AGGACTGCTG GAAGACATCA CTGCCAATTC TGCCCTTGGG
    GAGGAGGTGG AGGCCAACCT AAAAGCCCTC TGCAAATCCA ATGAGTTTGA AAAATACCGT
    TTATTTATCG GGGACCTGGA CAAAGTAGTC AACCTGTTGC TGTCACTCTC TGGTCGGCTG
    GCACGGGTGG AGAATGCTCT CAACAGCATC GATTCAGAGG CCAACCAGGA GAAGTTGGTG
    CTGATAGAGA AGAAGCAGCA GCTGACAGGG CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG
    CATGTGGACC GTCGGGAGAA GTTGGTGTTT GGCATGGTCT CCCGTTACCT GCCTCAGGAC
    CAGCTCCAAG ATTACCAGCA CTTTGTGAAG ATGAAATCTG CTCTCATCAT TGAACAGCGA
    GAGCTGGAAG AGAAGATCAA GCTCGGGGAA GAACAGCTCA AATGTCTCAG GGAAAGCCTG
    CTCCTGGGGC CCAGTAATTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820252.1
    CDS54..4577
    Translation

    Target ORF information:

    RefSeq Version XM_010821950.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821950.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATCGAAG ATGGAGGCAA GGCAGCCTTG TCCCAGAAGA TGAGGACTGG TGATGAGCTG 
    GTGAATATCA ATGGCACTCC ATTGTACGGC TCCCGCCAAG AGGCTCTCAT CCTCATCAAA
    GGCTCCTTCC GGACCCTCAA GCTGATTGTC AGGAGGAGAA ACGCCCCTGT CAGTAGGCCG
    CACTCATGGC ATGTGGCCAA GCTGCTGGAG GGATGCCCTG AAGCGGCCAC CACCATGCAT
    TTCCCTTCTG AAGCCTTCAG CTTGTCCTGG CATTCCGGCT GCAACACAAG TGACGTGTGT
    GTGCAGTGGT GTCCACTCTC CCGGCACTGC AGCACTGAGA AAAGCAGCTC CATTGGCAGT
    ATGGAGAGCC TGGAGCAACC AGGCCAAGCC ACCTATGAGG GCCATCTTTT GCCCATTGAC
    CAGAGCACAT ACCCCAACCA GCGTGATTCA GCCTATAGCT CCTTCTCAGC CAGCTCAAAT
    GCCTCTGACT GTGCCCTTTC CCTCAGGCCA GAGGAGCCAG CCTCTGTGGA CTGTATCATG
    CAAGGCCCAG GGCCAACTAA AGCCCCTAAT AGTCGCCCTA ATGTGGTCGA GACCTCAGGA
    GGTGGCTGGC GCACCAGTGG GGGTCACCTG ACTGCTAGCT CCCAGATGTC ATCCTGTCCT
    CAGGAGGTCT CCCACTCAGA GCCTGCCAAG GCTGCCAGGG GTCCCCCTCA ACCTCCAGTG
    AGGCGAGACA GTCTTCAGGC CTCCAGAGCC CAACTCTTCA ATGGAGAGCA GTGCAGGGCT
    TCTGAGCTTA TAGATTCCTT GCAACAGAAG GAGAAACCTA GCTTGGACAC CATGCTATCC
    CCAAGGAACC CTAATAGGTT CTGCTGCCTC AGCGGGCAAG ACCAAGTGAC AAATGAAGGC
    CATCAGAACT GTGAGCTCAG CCAACCTCCT GAATCTAACC AGAAGGGCTC TGAGAATCTA
    CCGATGGAGG CCTCAGCCAA AGCTGTTGGA GTCCCAAAAG CATGTGACAA AACTTCCAGC
    ATAGGTTCCA GCCCACTCAA CCAGGCTTCA GCAGAGCTAG CTAAGACTTC TTCTTTTGGC
    AGCTCTCCAC ACCTCACAGG ACCCACAGGG CATCGCCATA GTGCCCCTGA ACAGCTGCTG
    GTGTCCCACT TGCAGCGTGT GCACCTTGAT ACTAGGGACA GCAGGGGGAC AGAGCTCCAA
    GCTGGGCAGG ATGGGCAGGA GTGGACCGTA TCCCCTTTGC ACATGCACAG TAGCCACACA
    AGCAAGAAAA GCCCATGTGC CCCTGCAGGA GGAACCCAGG ACCAGCCGAG CAAAGACAGA
    AAGACCAAGC CAGTGGATGA TAGACCTCTG GGTTCAGGTC ATCAGAGCCC AAGCAGTTCC
    CCACATGGAG AGGCTGACGG ACACCATCCA GAAAAAGGCT TCCAGGATCC AAACAGAGCA
    AGCAGAGCTG ATAGTGAACT GGTCAGCCAG CAGTCCTCTG GCTCCCTTGC TCAACAAACC
    AGGGACTGTT CTTCAACCTC TAAAGTAGCT GGCAGCACAG AGGCAACTGA GGAAGGGGAC
    AGTGAGCCCA AGGAGTGTGG CCGGGTGGGC AGTAAGCGAA ATGGAGGGTT CCGGGGCCGC
    TCGATCCAAA ACCGGCGGAA GAGCGAACGT TTTGCTACCA ATCTACGTAA TGAAATTCAG
    AGGAGGAAGG CCCAGCTCCA GAAAGGCAAG AGTCCCTTGT CACAGCTGTG TTACACCAAG
    GAGCCAGTGG AAGAGACCGA GGAGACCCTG GAAAGTCCTC CACTTCCTTC CTCTAACTCA
    GCCCTTCTAT CTTCATATAA AAAACCACCA AGCCCCAGAG ACAAGCTCTT CAACAAGAGC
    ATGATACTCA GGGCTAGGTC TTCAGAGTGC CTCAGTCAAG CCCCTGAGGG TCAAGAATCT
    AGGATTGGCT TGGAGGGACG AGTGAGTCCT GGCCAGAGAT CTGGCCAGTC CTCTTTGAGC
    CCAAACACCT GGTGGAAAGC ATCTGACCCA TCCTCGTCAG ACTCTGAAAA AACAAACGTT
    CACCATGGAG TCTGTGGAGG TCACTGGAGA TGGTCACCAG AGCACAACCT GCAGCCACAT
    GTGGCACTAG CCATGGAAGG CCCTTCCAAC CCAGGTGACA AGGAATTGAA AGCGTCTACT
    GTCCAAGCTG GGGAGGAAGC CATCCTCTTG CCCTTTGCAG ACAGAAGAAA GTTCTTTGAA
    GAGAGTAGCA AATCCTTATC TACATCTCAT TTGCCAGGTT TAACCACTCA TAACAAGACT
    TTTACCCAGA GACCAAAACC TATTGACCAA AGCTTTCAGC CAATGAGCTC CAGCTATAGA
    GAATTGAGGC GCCATCCCAT AGACCAATCA TATCACTCCA CAGACCAACC CTATCATGCC
    ACAGACCAAT CATATCATTC CATATCACCC CTTCAGTCAG AAACTCCCAC TTACACAGAA
    TGTTTTGCAA GCAAAGGTCT AGAACAATCC ATGTGCTGTA AGCCACTACA CTGTGGTGAT
    TTTGATTACC ACAGGACGTG CTCTTACTCC TGCAATGTCC AGGGAGCTGT AGTCCATGAC
    CCTTGCATTT ATTGTTCTGG GGAAATCTGT CCTGCTTTGC GAAAGAGAAA TATGATTCCA
    AACTGCTACA ATTGCCGGTG CCACCATCAC CAGTGCATCC GGTGTTCAGC TTGCTGTCAT
    AATCCCCAGC ATGGTACCCT CGAGGACAGC AGCTTGAAAC CTGGCAACAC TTGGAAACCC
    AGGAAGCCGA CAGTGCAGGA ATTTCCTGGG GACAAATGGA AACCAATAAC AGGAAACAGG
    AAGACCAGCC AGTCAGGGAG GGAAATGGCT CATTCCAAGG CTAGCTTTTC ATGGGCAACC
    CCATTCCATC CTTGCCTTGA GAACCCAGCA CTGGACTTGT CAAACTACCG AGCAGTCTCT
    TCTCTTGACC TCCTTGGAGA CTTTAAGCAC TCCTCCAAAA AAACAGAGGA AACCTCCGTT
    TATGAAGAGG AGAGCTCCCT AGCCTCCATG CCTCGCCCAC TGCGCAGCCG TGCCTTCTCA
    GAAAGCCACA TCACCCTGGA TCCTCAGAGT TCCCAGGTCT GGGAGCAGCA TAGGAAGGAG
    CTCTTCAACA AAGTTGATGA GACCCAGCCA GATGCTCTGG GTGCCAGGAA GAAGGCCTTT
    CCTCCTCCTC GTCCTCCTCC TCCCAACTGG GAGAAGTACA GGCTCTTCCG TGCAGCCCAG
    CAACAGCAAC AGCAGCAAAA GCAGCAGCAG CAAGAGGAGG AAGAAGAGGA GGAGGAGGAA
    GAGGAAGAAG AAGAGGAGGA AGGGGTAGAG GAGGAGGAAG AAGAGGAGGA AGCGGGGGAG
    GAGGAGGAGC TGCCACCCCA GTATTTCAGT TCAGAAACCA CTGGTTCTGG TGCCCTCCAT
    CCTGAGGAGG TTCTGGAGCA GCCCCAGCTT CCGGCCTTTG GCCACCTGGA GGCCCCGAGG
    CAGGGCTCAC AAAGCCTCCC AGCAGAGCCA GAATCCTTTG CTCTGCGTTC CAGTGATTTC
    CTGCCTCCAG TAAGGGGCCA CTTGGGATCT CAAGCTGAAA AGGCTCAACC TCCTTGTTAT
    TATGGCATTG GTGGGCTTTG GAGGACATCA GAGCAGGAGA CCACTGATTC CAAACCAGAA
    GCTTTGATGG CGGAAGAGTC CAAATCCAAA CCAGCATGGA GCCAGAGCTA CTTCTTTCCT
    GAGGAGCAGT TCTCTTTCAT GGGCTGGGGC TCAGATAAGC AGCACCTCAA CCCTTCAGGC
    TTCAGTGAAC CCAAGACAAC AGGACAAGAT TTTCAGCACT TCTCACCTCC TCAGGGGGCC
    CCAGGGATCC CTACCTCTTA TTCAGCTTAT TACAATATTT CTGTGGCCAA AGCAGAGCTG
    TTGAACAAGT TGAAAGACCA ACCTGAGATG TTAGAGATTG GCCTAGGAGA GGAGGAAATT
    GACCACGAAC TGGCTCAAAA AAAGGTACAG CTTATTGACA GCATTGGCAG AAAACTCTCT
    GTCCTGCGGG AGGCCCAGCG AGGACTGCTG GAAGACATCA CTGCCAATTC TGCCCTTGGG
    GAGGAGGTGG AGGCCAACCT AAAAGCCCTC TGCAAATCCA ATGAGTTTGA AAAATACCGT
    TTATTTATCG GGGACCTGGA CAAAGTAGTC AACCTGTTGC TGTCACTCTC TGGTCGGCTG
    GCACGGGTGG AGAATGCTCT CAACAGCATC GATTCAGAGG CCAACCAGGA GAAGTTGGTG
    CTGATAGAGA AGAAGCAGCA GCTGACAGGG CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG
    CATGTGGACC GTCGGGAGAA GTTGGTGTTT GGCATGGTCT CCCGTTACCT GCCTCAGGAC
    CAGCTCCAAG ATTACCAGCA CTTTGTGAAG ATGAAATCTG CTCTCATCAT TGAACAGCGA
    GAGCTGGAAG AGAAGATCAA GCTCGGGGAA GAACAGCTCA AATGTCTCAG GGAAAGCCTG
    CTCCTGGGGC CCAGTAATTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49994
    Clone ID Related Accession (Same CDS sequence) XM_015470468.1 , XM_010821950.2 , XM_010821950.3
    Accession Version XM_010821950.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821950.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATCGAAG ATGGAGGCAA GGCAGCCTTG TCCCAGAAGA TGAGGACTGG TGATGAGCTG 
    GTGAATATCA ATGGCACTCC ATTGTACGGC TCCCGCCAAG AGGCTCTCAT CCTCATCAAA
    GGCTCCTTCC GGACCCTCAA GCTGATTGTC AGGAGGAGAA ACGCCCCTGT CAGTAGGCCG
    CACTCATGGC ATGTGGCCAA GCTGCTGGAG GGATGCCCTG AAGCGGCCAC CACCATGCAT
    TTCCCTTCTG AAGCCTTCAG CTTGTCCTGG CATTCCGGCT GCAACACAAG TGACGTGTGT
    GTGCAGTGGT GTCCACTCTC CCGGCACTGC AGCACTGAGA AAAGCAGCTC CATTGGCAGT
    ATGGAGAGCC TGGAGCAACC AGGCCAAGCC ACCTATGAGG GCCATCTTTT GCCCATTGAC
    CAGAGCACAT ACCCCAACCA GCGTGATTCA GCCTATAGCT CCTTCTCAGC CAGCTCAAAT
    GCCTCTGACT GTGCCCTTTC CCTCAGGCCA GAGGAGCCAG CCTCTGTGGA CTGTATCATG
    CAAGGCCCAG GGCCAACTAA AGCCCCTAAT AGTCGCCCTA ATGTGGTCGA GACCTCAGGA
    GGTGGCTGGC GCACCAGTGG GGGTCACCTG ACTGCTAGCT CCCAGATGTC ATCCTGTCCT
    CAGGAGGTCT CCCACTCAGA GCCTGCCAAG GCTGCCAGGG GTCCCCCTCA ACCTCCAGTG
    AGGCGAGACA GTCTTCAGGC CTCCAGAGCC CAACTCTTCA ATGGAGAGCA GTGCAGGGCT
    TCTGAGCTTA TAGATTCCTT GCAACAGAAG GAGAAACCTA GCTTGGACAC CATGCTATCC
    CCAAGGAACC CTAATAGGTT CTGCTGCCTC AGCGGGCAAG ACCAAGTGAC AAATGAAGGC
    CATCAGAACT GTGAGCTCAG CCAACCTCCT GAATCTAACC AGAAGGGCTC TGAGAATCTA
    CCGATGGAGG CCTCAGCCAA AGCTGTTGGA GTCCCAAAAG CATGTGACAA AACTTCCAGC
    ATAGGTTCCA GCCCACTCAA CCAGGCTTCA GCAGAGCTAG CTAAGACTTC TTCTTTTGGC
    AGCTCTCCAC ACCTCACAGG ACCCACAGGG CATCGCCATA GTGCCCCTGA ACAGCTGCTG
    GTGTCCCACT TGCAGCGTGT GCACCTTGAT ACTAGGGACA GCAGGGGGAC AGAGCTCCAA
    GCTGGGCAGG ATGGGCAGGA GTGGACCGTA TCCCCTTTGC ACATGCACAG TAGCCACACA
    AGCAAGAAAA GCCCATGTGC CCCTGCAGGA GGAACCCAGG ACCAGCCGAG CAAAGACAGA
    AAGACCAAGC CAGTGGATGA TAGACCTCTG GGTTCAGGTC ATCAGAGCCC AAGCAGTTCC
    CCACATGGAG AGGCTGACGG ACACCATCCA GAAAAAGGCT TCCAGGATCC AAACAGAGCA
    AGCAGAGCTG ATAGTGAACT GGTCAGCCAG CAGTCCTCTG GCTCCCTTGC TCAACAAACC
    AGGGACTGTT CTTCAACCTC TAAAGTAGCT GGCAGCACAG AGGCAACTGA GGAAGGGGAC
    AGTGAGCCCA AGGAGTGTGG CCGGGTGGGC AGTAAGCGAA ATGGAGGGTT CCGGGGCCGC
    TCGATCCAAA ACCGGCGGAA GAGCGAACGT TTTGCTACCA ATCTACGTAA TGAAATTCAG
    AGGAGGAAGG CCCAGCTCCA GAAAGGCAAG AGTCCCTTGT CACAGCTGTG TTACACCAAG
    GAGCCAGTGG AAGAGACCGA GGAGACCCTG GAAAGTCCTC CACTTCCTTC CTCTAACTCA
    GCCCTTCTAT CTTCATATAA AAAACCACCA AGCCCCAGAG ACAAGCTCTT CAACAAGAGC
    ATGATACTCA GGGCTAGGTC TTCAGAGTGC CTCAGTCAAG CCCCTGAGGG TCAAGAATCT
    AGGATTGGCT TGGAGGGACG AGTGAGTCCT GGCCAGAGAT CTGGCCAGTC CTCTTTGAGC
    CCAAACACCT GGTGGAAAGC ATCTGACCCA TCCTCGTCAG ACTCTGAAAA AACAAACGTT
    CACCATGGAG TCTGTGGAGG TCACTGGAGA TGGTCACCAG AGCACAACCT GCAGCCACAT
    GTGGCACTAG CCATGGAAGG CCCTTCCAAC CCAGGTGACA AGGAATTGAA AGCGTCTACT
    GTCCAAGCTG GGGAGGAAGC CATCCTCTTG CCCTTTGCAG ACAGAAGAAA GTTCTTTGAA
    GAGAGTAGCA AATCCTTATC TACATCTCAT TTGCCAGGTT TAACCACTCA TAACAAGACT
    TTTACCCAGA GACCAAAACC TATTGACCAA AGCTTTCAGC CAATGAGCTC CAGCTATAGA
    GAATTGAGGC GCCATCCCAT AGACCAATCA TATCACTCCA CAGACCAACC CTATCATGCC
    ACAGACCAAT CATATCATTC CATATCACCC CTTCAGTCAG AAACTCCCAC TTACACAGAA
    TGTTTTGCAA GCAAAGGTCT AGAACAATCC ATGTGCTGTA AGCCACTACA CTGTGGTGAT
    TTTGATTACC ACAGGACGTG CTCTTACTCC TGCAATGTCC AGGGAGCTGT AGTCCATGAC
    CCTTGCATTT ATTGTTCTGG GGAAATCTGT CCTGCTTTGC GAAAGAGAAA TATGATTCCA
    AACTGCTACA ATTGCCGGTG CCACCATCAC CAGTGCATCC GGTGTTCAGC TTGCTGTCAT
    AATCCCCAGC ATGGTACCCT CGAGGACAGC AGCTTGAAAC CTGGCAACAC TTGGAAACCC
    AGGAAGCCGA CAGTGCAGGA ATTTCCTGGG GACAAATGGA AACCAATAAC AGGAAACAGG
    AAGACCAGCC AGTCAGGGAG GGAAATGGCT CATTCCAAGG CTAGCTTTTC ATGGGCAACC
    CCATTCCATC CTTGCCTTGA GAACCCAGCA CTGGACTTGT CAAACTACCG AGCAGTCTCT
    TCTCTTGACC TCCTTGGAGA CTTTAAGCAC TCCTCCAAAA AAACAGAGGA AACCTCCGTT
    TATGAAGAGG AGAGCTCCCT AGCCTCCATG CCTCGCCCAC TGCGCAGCCG TGCCTTCTCA
    GAAAGCCACA TCACCCTGGA TCCTCAGAGT TCCCAGGTCT GGGAGCAGCA TAGGAAGGAG
    CTCTTCAACA AAGTTGATGA GACCCAGCCA GATGCTCTGG GTGCCAGGAA GAAGGCCTTT
    CCTCCTCCTC GTCCTCCTCC TCCCAACTGG GAGAAGTACA GGCTCTTCCG TGCAGCCCAG
    CAACAGCAAC AGCAGCAAAA GCAGCAGCAG CAAGAGGAGG AAGAAGAGGA GGAGGAGGAA
    GAGGAAGAAG AAGAGGAGGA AGGGGTAGAG GAGGAGGAAG AAGAGGAGGA AGCGGGGGAG
    GAGGAGGAGC TGCCACCCCA GTATTTCAGT TCAGAAACCA CTGGTTCTGG TGCCCTCCAT
    CCTGAGGAGG TTCTGGAGCA GCCCCAGCTT CCGGCCTTTG GCCACCTGGA GGCCCCGAGG
    CAGGGCTCAC AAAGCCTCCC AGCAGAGCCA GAATCCTTTG CTCTGCGTTC CAGTGATTTC
    CTGCCTCCAG TAAGGGGCCA CTTGGGATCT CAAGCTGAAA AGGCTCAACC TCCTTGTTAT
    TATGGCATTG GTGGGCTTTG GAGGACATCA GAGCAGGAGA CCACTGATTC CAAACCAGAA
    GCTTTGATGG CGGAAGAGTC CAAATCCAAA CCAGCATGGA GCCAGAGCTA CTTCTTTCCT
    GAGGAGCAGT TCTCTTTCAT GGGCTGGGGC TCAGATAAGC AGCACCTCAA CCCTTCAGGC
    TTCAGTGAAC CCAAGACAAC AGGACAAGAT TTTCAGCACT TCTCACCTCC TCAGGGGGCC
    CCAGGGATCC CTACCTCTTA TTCAGCTTAT TACAATATTT CTGTGGCCAA AGCAGAGCTG
    TTGAACAAGT TGAAAGACCA ACCTGAGATG TTAGAGATTG GCCTAGGAGA GGAGGAAATT
    GACCACGAAC TGGCTCAAAA AAAGGTACAG CTTATTGACA GCATTGGCAG AAAACTCTCT
    GTCCTGCGGG AGGCCCAGCG AGGACTGCTG GAAGACATCA CTGCCAATTC TGCCCTTGGG
    GAGGAGGTGG AGGCCAACCT AAAAGCCCTC TGCAAATCCA ATGAGTTTGA AAAATACCGT
    TTATTTATCG GGGACCTGGA CAAAGTAGTC AACCTGTTGC TGTCACTCTC TGGTCGGCTG
    GCACGGGTGG AGAATGCTCT CAACAGCATC GATTCAGAGG CCAACCAGGA GAAGTTGGTG
    CTGATAGAGA AGAAGCAGCA GCTGACAGGG CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG
    CATGTGGACC GTCGGGAGAA GTTGGTGTTT GGCATGGTCT CCCGTTACCT GCCTCAGGAC
    CAGCTCCAAG ATTACCAGCA CTTTGTGAAG ATGAAATCTG CTCTCATCAT TGAACAGCGA
    GAGCTGGAAG AGAAGATCAA GCTCGGGGAA GAACAGCTCA AATGTCTCAG GGAAAGCCTG
    CTCCTGGGGC CCAGTAATTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820252.1
    CDS16..4539
    Translation

    Target ORF information:

    RefSeq Version XM_010821950.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821950.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATCGAAG ATGGAGGCAA GGCAGCCTTG TCCCAGAAGA TGAGGACTGG TGATGAGCTG 
    GTGAATATCA ATGGCACTCC ATTGTACGGC TCCCGCCAAG AGGCTCTCAT CCTCATCAAA
    GGCTCCTTCC GGACCCTCAA GCTGATTGTC AGGAGGAGAA ACGCCCCTGT CAGTAGGCCG
    CACTCATGGC ATGTGGCCAA GCTGCTGGAG GGATGCCCTG AAGCGGCCAC CACCATGCAT
    TTCCCTTCTG AAGCCTTCAG CTTGTCCTGG CATTCCGGCT GCAACACAAG TGACGTGTGT
    GTGCAGTGGT GTCCACTCTC CCGGCACTGC AGCACTGAGA AAAGCAGCTC CATTGGCAGT
    ATGGAGAGCC TGGAGCAACC AGGCCAAGCC ACCTATGAGG GCCATCTTTT GCCCATTGAC
    CAGAGCACAT ACCCCAACCA GCGTGATTCA GCCTATAGCT CCTTCTCAGC CAGCTCAAAT
    GCCTCTGACT GTGCCCTTTC CCTCAGGCCA GAGGAGCCAG CCTCTGTGGA CTGTATCATG
    CAAGGCCCAG GGCCAACTAA AGCCCCTAAT AGTCGCCCTA ATGTGGTCGA GACCTCAGGA
    GGTGGCTGGC GCACCAGTGG GGGTCACCTG ACTGCTAGCT CCCAGATGTC ATCCTGTCCT
    CAGGAGGTCT CCCACTCAGA GCCTGCCAAG GCTGCCAGGG GTCCCCCTCA ACCTCCAGTG
    AGGCGAGACA GTCTTCAGGC CTCCAGAGCC CAACTCTTCA ATGGAGAGCA GTGCAGGGCT
    TCTGAGCTTA TAGATTCCTT GCAACAGAAG GAGAAACCTA GCTTGGACAC CATGCTATCC
    CCAAGGAACC CTAATAGGTT CTGCTGCCTC AGCGGGCAAG ACCAAGTGAC AAATGAAGGC
    CATCAGAACT GTGAGCTCAG CCAACCTCCT GAATCTAACC AGAAGGGCTC TGAGAATCTA
    CCGATGGAGG CCTCAGCCAA AGCTGTTGGA GTCCCAAAAG CATGTGACAA AACTTCCAGC
    ATAGGTTCCA GCCCACTCAA CCAGGCTTCA GCAGAGCTAG CTAAGACTTC TTCTTTTGGC
    AGCTCTCCAC ACCTCACAGG ACCCACAGGG CATCGCCATA GTGCCCCTGA ACAGCTGCTG
    GTGTCCCACT TGCAGCGTGT GCACCTTGAT ACTAGGGACA GCAGGGGGAC AGAGCTCCAA
    GCTGGGCAGG ATGGGCAGGA GTGGACCGTA TCCCCTTTGC ACATGCACAG TAGCCACACA
    AGCAAGAAAA GCCCATGTGC CCCTGCAGGA GGAACCCAGG ACCAGCCGAG CAAAGACAGA
    AAGACCAAGC CAGTGGATGA TAGACCTCTG GGTTCAGGTC ATCAGAGCCC AAGCAGTTCC
    CCACATGGAG AGGCTGACGG ACACCATCCA GAAAAAGGCT TCCAGGATCC AAACAGAGCA
    AGCAGAGCTG ATAGTGAACT GGTCAGCCAG CAGTCCTCTG GCTCCCTTGC TCAACAAACC
    AGGGACTGTT CTTCAACCTC TAAAGTAGCT GGCAGCACAG AGGCAACTGA GGAAGGGGAC
    AGTGAGCCCA AGGAGTGTGG CCGGGTGGGC AGTAAGCGAA ATGGAGGGTT CCGGGGCCGC
    TCGATCCAAA ACCGGCGGAA GAGCGAACGT TTTGCTACCA ATCTACGTAA TGAAATTCAG
    AGGAGGAAGG CCCAGCTCCA GAAAGGCAAG AGTCCCTTGT CACAGCTGTG TTACACCAAG
    GAGCCAGTGG AAGAGACCGA GGAGACCCTG GAAAGTCCTC CACTTCCTTC CTCTAACTCA
    GCCCTTCTAT CTTCATATAA AAAACCACCA AGCCCCAGAG ACAAGCTCTT CAACAAGAGC
    ATGATACTCA GGGCTAGGTC TTCAGAGTGC CTCAGTCAAG CCCCTGAGGG TCAAGAATCT
    AGGATTGGCT TGGAGGGACG AGTGAGTCCT GGCCAGAGAT CTGGCCAGTC CTCTTTGAGC
    CCAAACACCT GGTGGAAAGC ATCTGACCCA TCCTCGTCAG ACTCTGAAAA AACAAACGTT
    CACCATGGAG TCTGTGGAGG TCACTGGAGA TGGTCACCAG AGCACAACCT GCAGCCACAT
    GTGGCACTAG CCATGGAAGG CCCTTCCAAC CCAGGTGACA AGGAATTGAA AGCGTCTACT
    GTCCAAGCTG GGGAGGAAGC CATCCTCTTG CCCTTTGCAG ACAGAAGAAA GTTCTTTGAA
    GAGAGTAGCA AATCCTTATC TACATCTCAT TTGCCAGGTT TAACCACTCA TAACAAGACT
    TTTACCCAGA GACCAAAACC TATTGACCAA AGCTTTCAGC CAATGAGCTC CAGCTATAGA
    GAATTGAGGC GCCATCCCAT AGACCAATCA TATCACTCCA CAGACCAACC CTATCATGCC
    ACAGACCAAT CATATCATTC CATATCACCC CTTCAGTCAG AAACTCCCAC TTACACAGAA
    TGTTTTGCAA GCAAAGGTCT AGAACAATCC ATGTGCTGTA AGCCACTACA CTGTGGTGAT
    TTTGATTACC ACAGGACGTG CTCTTACTCC TGCAATGTCC AGGGAGCTGT AGTCCATGAC
    CCTTGCATTT ATTGTTCTGG GGAAATCTGT CCTGCTTTGC GAAAGAGAAA TATGATTCCA
    AACTGCTACA ATTGCCGGTG CCACCATCAC CAGTGCATCC GGTGTTCAGC TTGCTGTCAT
    AATCCCCAGC ATGGTACCCT CGAGGACAGC AGCTTGAAAC CTGGCAACAC TTGGAAACCC
    AGGAAGCCGA CAGTGCAGGA ATTTCCTGGG GACAAATGGA AACCAATAAC AGGAAACAGG
    AAGACCAGCC AGTCAGGGAG GGAAATGGCT CATTCCAAGG CTAGCTTTTC ATGGGCAACC
    CCATTCCATC CTTGCCTTGA GAACCCAGCA CTGGACTTGT CAAACTACCG AGCAGTCTCT
    TCTCTTGACC TCCTTGGAGA CTTTAAGCAC TCCTCCAAAA AAACAGAGGA AACCTCCGTT
    TATGAAGAGG AGAGCTCCCT AGCCTCCATG CCTCGCCCAC TGCGCAGCCG TGCCTTCTCA
    GAAAGCCACA TCACCCTGGA TCCTCAGAGT TCCCAGGTCT GGGAGCAGCA TAGGAAGGAG
    CTCTTCAACA AAGTTGATGA GACCCAGCCA GATGCTCTGG GTGCCAGGAA GAAGGCCTTT
    CCTCCTCCTC GTCCTCCTCC TCCCAACTGG GAGAAGTACA GGCTCTTCCG TGCAGCCCAG
    CAACAGCAAC AGCAGCAAAA GCAGCAGCAG CAAGAGGAGG AAGAAGAGGA GGAGGAGGAA
    GAGGAAGAAG AAGAGGAGGA AGGGGTAGAG GAGGAGGAAG AAGAGGAGGA AGCGGGGGAG
    GAGGAGGAGC TGCCACCCCA GTATTTCAGT TCAGAAACCA CTGGTTCTGG TGCCCTCCAT
    CCTGAGGAGG TTCTGGAGCA GCCCCAGCTT CCGGCCTTTG GCCACCTGGA GGCCCCGAGG
    CAGGGCTCAC AAAGCCTCCC AGCAGAGCCA GAATCCTTTG CTCTGCGTTC CAGTGATTTC
    CTGCCTCCAG TAAGGGGCCA CTTGGGATCT CAAGCTGAAA AGGCTCAACC TCCTTGTTAT
    TATGGCATTG GTGGGCTTTG GAGGACATCA GAGCAGGAGA CCACTGATTC CAAACCAGAA
    GCTTTGATGG CGGAAGAGTC CAAATCCAAA CCAGCATGGA GCCAGAGCTA CTTCTTTCCT
    GAGGAGCAGT TCTCTTTCAT GGGCTGGGGC TCAGATAAGC AGCACCTCAA CCCTTCAGGC
    TTCAGTGAAC CCAAGACAAC AGGACAAGAT TTTCAGCACT TCTCACCTCC TCAGGGGGCC
    CCAGGGATCC CTACCTCTTA TTCAGCTTAT TACAATATTT CTGTGGCCAA AGCAGAGCTG
    TTGAACAAGT TGAAAGACCA ACCTGAGATG TTAGAGATTG GCCTAGGAGA GGAGGAAATT
    GACCACGAAC TGGCTCAAAA AAAGGTACAG CTTATTGACA GCATTGGCAG AAAACTCTCT
    GTCCTGCGGG AGGCCCAGCG AGGACTGCTG GAAGACATCA CTGCCAATTC TGCCCTTGGG
    GAGGAGGTGG AGGCCAACCT AAAAGCCCTC TGCAAATCCA ATGAGTTTGA AAAATACCGT
    TTATTTATCG GGGACCTGGA CAAAGTAGTC AACCTGTTGC TGTCACTCTC TGGTCGGCTG
    GCACGGGTGG AGAATGCTCT CAACAGCATC GATTCAGAGG CCAACCAGGA GAAGTTGGTG
    CTGATAGAGA AGAAGCAGCA GCTGACAGGG CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG
    CATGTGGACC GTCGGGAGAA GTTGGTGTTT GGCATGGTCT CCCGTTACCT GCCTCAGGAC
    CAGCTCCAAG ATTACCAGCA CTTTGTGAAG ATGAAATCTG CTCTCATCAT TGAACAGCGA
    GAGCTGGAAG AGAAGATCAA GCTCGGGGAA GAACAGCTCA AATGTCTCAG GGAAAGCCTG
    CTCCTGGGGC CCAGTAATTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49995
    Clone ID Related Accession (Same CDS sequence) XM_010821952.2 , XM_015470470.1 , XM_010821952.3
    Accession Version XM_010821952.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104731.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
    GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGCAGCA CTGAGAAAAG CAGCTCCATT
    GGCAGTATGG AGAGCCTGGA GCAACCAGGC CAAGCCACCT ATGAGGGCCA TCTTTTGCCC
    ATTGACCAGA GCACATACCC CAACCAGCGT GATTCAGCCT ATAGCTCCTT CTCAGCCAGC
    TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGTGGACTGT
    ATCATGCAAG GCCCAGGGCC AACTAAAGCC CCTAATAGTC GCCCTAATGT GGTCGAGACC
    TCAGGAGGTG GCTGGCGCAC CAGTGGGGGT CACCTGACTG CTAGCTCCCA GATGTCATCC
    TGTCCTCAGG AGGTCTCCCA CTCAGAGCCT GCCAAGGCTG CCAGGGGTCC CCCTCAACCT
    CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAAC TCTTCAATGG AGAGCAGTGC
    AGGGCTTCTG AGCTTATAGA TTCCTTGCAA CAGAAGGAGA AACCTAGCTT GGACACCATG
    CTATCCCCAA GGAACCCTAA TAGGTTCTGC TGCCTCAGCG GGCAAGACCA AGTGACAAAT
    GAAGGCCATC AGAACTGTGA GCTCAGCCAA CCTCCTGAAT CTAACCAGAA GGGCTCTGAG
    AATCTACCGA TGGAGGCCTC AGCCAAAGCT GTTGGAGTCC CAAAAGCATG TGACAAAACT
    TCCAGCATAG GTTCCAGCCC ACTCAACCAG GCTTCAGCAG AGCTAGCTAA GACTTCTTCT
    TTTGGCAGCT CTCCACACCT CACAGGACCC ACAGGGCATC GCCATAGTGC CCCTGAACAG
    CTGCTGGTGT CCCACTTGCA GCGTGTGCAC CTTGATACTA GGGACAGCAG GGGGACAGAG
    CTCCAAGCTG GGCAGGATGG GCAGGAGTGG ACCGTATCCC CTTTGCACAT GCACAGTAGC
    CACACAAGCA AGAAAAGCCC ATGTGCCCCT GCAGGAGGAA CCCAGGACCA GCCGAGCAAA
    GACAGAAAGA CCAAGCCAGT GGATGATAGA CCTCTGGGTT CAGGTCATCA GAGCCCAAGC
    AGTTCCCCAC ATGGAGAGGC TGACGGACAC CATCCAGAAA AAGGCTTCCA GGATCCAAAC
    AGAGCAAGCA GAGCTGATAG TGAACTGGTC AGCCAGCAGT CCTCTGGCTC CCTTGCTCAA
    CAAACCAGGG ACTGTTCTTC AACCTCTAAA GTAGCTGGCA GCACAGAGGC AACTGAGGAA
    GGGGACAGTG AGCCCAAGGA GTGTGGCCGG GTGGGCAGTA AGCGAAATGG AGGGTTCCGG
    GGCCGCTCGA TCCAAAACCG GCGGAAGAGC GAACGTTTTG CTACCAATCT ACGTAATGAA
    ATTCAGAGGA GGAAGGCCCA GCTCCAGAAA GGCAAGAGTC CCTTGTCACA GCTGTGTTAC
    ACCAAGGAGC CAGTGGAAGA GACCGAGGAG ACCCTGGAAA GTCCTCCACT TCCTTCCTCT
    AACTCAGCCC TTCTATCTTC ATATAAAAAA CCACCAAGCC CCAGAGACAA GCTCTTCAAC
    AAGAGCATGA TACTCAGGGC TAGGTCTTCA GAGTGCCTCA GTCAAGCCCC TGAGGGTCAA
    GAATCTAGGA TTGGCTTGGA GGGACGAGTG AGTCCTGGCC AGAGATCTGG CCAGTCCTCT
    TTGAGCCCAA ACACCTGGTG GAAAGCATCT GACCCATCCT CGTCAGACTC TGAAAAAACA
    AACGTTCACC ATGGAGTCTG TGGAGGTCAC TGGAGATGGT CACCAGAGCA CAACCTGCAG
    CCACATGTGG CACTAGCCAT GGAAGGCCCT TCCAACCCAG GTGACAAGGA ATTGAAAGCG
    TCTACTGTCC AAGCTGGGGA GGAAGCCATC CTCTTGCCCT TTGCAGACAG AAGAAAGTTC
    TTTGAAGAGA GTAGCAAATC CTTATCTACA TCTCATTTGC CAGGTTTAAC CACTCATAAC
    AAGACTTTTA CCCAGAGACC AAAACCTATT GACCAAAGCT TTCAGCCAAT GAGCTCCAGC
    TATAGAGAAT TGAGGCGCCA TCCCATAGAC CAATCATATC ACTCCACAGA CCAACCCTAT
    CATGCCACAG ACCAATCATA TCATTCCATA TCACCCCTTC AGTCAGAAAC TCCCACTTAC
    ACAGAATGTT TTGCAAGCAA AGGTCTAGAA CAATCCATGT GCTGTAAGCC ACTACACTGT
    GGTGATTTTG ATTACCACAG GACGTGCTCT TACTCCTGCA ATGTCCAGGG AGCTGTAGTC
    CATGACCCTT GCATTTATTG TTCTGGGGAA ATCTGTCCTG CTTTGCGAAA GAGAAATATG
    ATTCCAAACT GCTACAATTG CCGGTGCCAC CATCACCAGT GCATCCGGTG TTCAGCTTGC
    TGTCATAATC CCCAGCATGG TACCCTCGAG GACAGCAGCT TGAAACCTGG CAACACTTGG
    AAACCCAGGA AGCCGACAGT GCAGGAATTT CCTGGGGACA AATGGAAACC AATAACAGGA
    AACAGGAAGA CCAGCCAGTC AGGGAGGGAA ATGGCTCATT CCAAGGCTAG CTTTTCATGG
    GCAACCCCAT TCCATCCTTG CCTTGAGAAC CCAGCACTGG ACTTGTCAAA CTACCGAGCA
    GTCTCTTCTC TTGACCTCCT TGGAGACTTT AAGCACTCCT CCAAAAAAAC AGAGGAAACC
    TCCGTTTATG AAGAGGAGAG CTCCCTAGCC TCCATGCCTC GCCCACTGCG CAGCCGTGCC
    TTCTCAGAAA GCCACATCAC CCTGGATCCT CAGAGTTCCC AGGTCTGGGA GCAGCATAGG
    AAGGAGCTCT TCAACAAAGT TGATGAGACC CAGCCAGATG CTCTGGGTGC CAGGAAGAAG
    GCCTTTCCTC CTCCTCGTCC TCCTCCTCCC AACTGGGAGA AGTACAGGCT CTTCCGTGCA
    GCCCAGCAAC AGCAACAGCA GCAAAAGCAG CAGCAGCAAG AGGAGGAAGA AGAGGAGGAG
    GAGGAAGAGG AAGAAGAAGA GGAGGAAGGG GTAGAGGAGG AGGAAGAAGA GGAGGAAGCG
    GGGGAGGAGG AGGAGCTGCC ACCCCAGTAT TTCAGTTCAG AAACCACTGG TTCTGGTGCC
    CTCCATCCTG AGGAGGTTCT GGAGCAGCCC CAGCTTCCGG CCTTTGGCCA CCTGGAGGCC
    CCGAGGCAGG GCTCACAAAG CCTCCCAGCA GAGCCAGAAT CCTTTGCTCT GCGTTCCAGT
    GATTTCCTGC CTCCAGTAAG GGGCCACTTG GGATCTCAAG CTGAAAAGGC TCAACCTCCT
    TGTTATTATG GCATTGGTGG GCTTTGGAGG ACATCAGAGC AGGAGACCAC TGATTCCAAA
    CCAGAAGCTT TGATGGCGGA AGAGTCCAAA TCCAAACCAG CATGGAGCCA GAGCTACTTC
    TTTCCTGAGG AGCAGTTCTC TTTCATGGGC TGGGGCTCAG ATAAGCAGCA CCTCAACCCT
    TCAGGCTTCA GTGAACCCAA GACAACAGGA CAAGATTTTC AGCACTTCTC ACCTCCTCAG
    GGGGCCCCAG GGATCCCTAC CTCTTATTCA GCTTATTACA ATATTTCTGT GGCCAAAGCA
    GAGCTGTTGA ACAAGTTGAA AGACCAACCT GAGATGTTAG AGATTGGCCT AGGAGAGGAG
    GAAATTGACC ACGAACTGGC TCAAAAAAAG GTACAGCTTA TTGACAGCAT TGGCAGAAAA
    CTCTCTGTCC TGCGGGAGGC CCAGCGAGGA CTGCTGGAAG ACATCACTGC CAATTCTGCC
    CTTGGGGAGG AGGTGGAGGC CAACCTAAAA GCCCTCTGCA AATCCAATGA GTTTGAAAAA
    TACCGTTTAT TTATCGGGGA CCTGGACAAA GTAGTCAACC TGTTGCTGTC ACTCTCTGGT
    CGGCTGGCAC GGGTGGAGAA TGCTCTCAAC AGCATCGATT CAGAGGCCAA CCAGGAGAAG
    TTGGTGCTGA TAGAGAAGAA GCAGCAGCTG ACAGGGCAGT TGGCAGATGC CAAGGAGCTG
    AAGGAGCATG TGGACCGTCG GGAGAAGTTG GTGTTTGGCA TGGTCTCCCG TTACCTGCCT
    CAGGACCAGC TCCAAGATTA CCAGCACTTT GTGAAGATGA AATCTGCTCT CATCATTGAA
    CAGCGAGAGC TGGAAGAGAA GATCAAGCTC GGGGAAGAAC AGCTCAAATG TCTCAGGGAA
    AGCCTGCTCC TGGGGCCCAG TAATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820254.1
    CDS538..4827
    Translation

    Target ORF information:

    RefSeq Version XM_010821952.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
    GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGCAGCA CTGAGAAAAG CAGCTCCATT
    GGCAGTATGG AGAGCCTGGA GCAACCAGGC CAAGCCACCT ATGAGGGCCA TCTTTTGCCC
    ATTGACCAGA GCACATACCC CAACCAGCGT GATTCAGCCT ATAGCTCCTT CTCAGCCAGC
    TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGTGGACTGT
    ATCATGCAAG GCCCAGGGCC AACTAAAGCC CCTAATAGTC GCCCTAATGT GGTCGAGACC
    TCAGGAGGTG GCTGGCGCAC CAGTGGGGGT CACCTGACTG CTAGCTCCCA GATGTCATCC
    TGTCCTCAGG AGGTCTCCCA CTCAGAGCCT GCCAAGGCTG CCAGGGGTCC CCCTCAACCT
    CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAAC TCTTCAATGG AGAGCAGTGC
    AGGGCTTCTG AGCTTATAGA TTCCTTGCAA CAGAAGGAGA AACCTAGCTT GGACACCATG
    CTATCCCCAA GGAACCCTAA TAGGTTCTGC TGCCTCAGCG GGCAAGACCA AGTGACAAAT
    GAAGGCCATC AGAACTGTGA GCTCAGCCAA CCTCCTGAAT CTAACCAGAA GGGCTCTGAG
    AATCTACCGA TGGAGGCCTC AGCCAAAGCT GTTGGAGTCC CAAAAGCATG TGACAAAACT
    TCCAGCATAG GTTCCAGCCC ACTCAACCAG GCTTCAGCAG AGCTAGCTAA GACTTCTTCT
    TTTGGCAGCT CTCCACACCT CACAGGACCC ACAGGGCATC GCCATAGTGC CCCTGAACAG
    CTGCTGGTGT CCCACTTGCA GCGTGTGCAC CTTGATACTA GGGACAGCAG GGGGACAGAG
    CTCCAAGCTG GGCAGGATGG GCAGGAGTGG ACCGTATCCC CTTTGCACAT GCACAGTAGC
    CACACAAGCA AGAAAAGCCC ATGTGCCCCT GCAGGAGGAA CCCAGGACCA GCCGAGCAAA
    GACAGAAAGA CCAAGCCAGT GGATGATAGA CCTCTGGGTT CAGGTCATCA GAGCCCAAGC
    AGTTCCCCAC ATGGAGAGGC TGACGGACAC CATCCAGAAA AAGGCTTCCA GGATCCAAAC
    AGAGCAAGCA GAGCTGATAG TGAACTGGTC AGCCAGCAGT CCTCTGGCTC CCTTGCTCAA
    CAAACCAGGG ACTGTTCTTC AACCTCTAAA GTAGCTGGCA GCACAGAGGC AACTGAGGAA
    GGGGACAGTG AGCCCAAGGA GTGTGGCCGG GTGGGCAGTA AGCGAAATGG AGGGTTCCGG
    GGCCGCTCGA TCCAAAACCG GCGGAAGAGC GAACGTTTTG CTACCAATCT ACGTAATGAA
    ATTCAGAGGA GGAAGGCCCA GCTCCAGAAA GGCAAGAGTC CCTTGTCACA GCTGTGTTAC
    ACCAAGGAGC CAGTGGAAGA GACCGAGGAG ACCCTGGAAA GTCCTCCACT TCCTTCCTCT
    AACTCAGCCC TTCTATCTTC ATATAAAAAA CCACCAAGCC CCAGAGACAA GCTCTTCAAC
    AAGAGCATGA TACTCAGGGC TAGGTCTTCA GAGTGCCTCA GTCAAGCCCC TGAGGGTCAA
    GAATCTAGGA TTGGCTTGGA GGGACGAGTG AGTCCTGGCC AGAGATCTGG CCAGTCCTCT
    TTGAGCCCAA ACACCTGGTG GAAAGCATCT GACCCATCCT CGTCAGACTC TGAAAAAACA
    AACGTTCACC ATGGAGTCTG TGGAGGTCAC TGGAGATGGT CACCAGAGCA CAACCTGCAG
    CCACATGTGG CACTAGCCAT GGAAGGCCCT TCCAACCCAG GTGACAAGGA ATTGAAAGCG
    TCTACTGTCC AAGCTGGGGA GGAAGCCATC CTCTTGCCCT TTGCAGACAG AAGAAAGTTC
    TTTGAAGAGA GTAGCAAATC CTTATCTACA TCTCATTTGC CAGGTTTAAC CACTCATAAC
    AAGACTTTTA CCCAGAGACC AAAACCTATT GACCAAAGCT TTCAGCCAAT GAGCTCCAGC
    TATAGAGAAT TGAGGCGCCA TCCCATAGAC CAATCATATC ACTCCACAGA CCAACCCTAT
    CATGCCACAG ACCAATCATA TCATTCCATA TCACCCCTTC AGTCAGAAAC TCCCACTTAC
    ACAGAATGTT TTGCAAGCAA AGGTCTAGAA CAATCCATGT GCTGTAAGCC ACTACACTGT
    GGTGATTTTG ATTACCACAG GACGTGCTCT TACTCCTGCA ATGTCCAGGG AGCTGTAGTC
    CATGACCCTT GCATTTATTG TTCTGGGGAA ATCTGTCCTG CTTTGCGAAA GAGAAATATG
    ATTCCAAACT GCTACAATTG CCGGTGCCAC CATCACCAGT GCATCCGGTG TTCAGCTTGC
    TGTCATAATC CCCAGCATGG TACCCTCGAG GACAGCAGCT TGAAACCTGG CAACACTTGG
    AAACCCAGGA AGCCGACAGT GCAGGAATTT CCTGGGGACA AATGGAAACC AATAACAGGA
    AACAGGAAGA CCAGCCAGTC AGGGAGGGAA ATGGCTCATT CCAAGGCTAG CTTTTCATGG
    GCAACCCCAT TCCATCCTTG CCTTGAGAAC CCAGCACTGG ACTTGTCAAA CTACCGAGCA
    GTCTCTTCTC TTGACCTCCT TGGAGACTTT AAGCACTCCT CCAAAAAAAC AGAGGAAACC
    TCCGTTTATG AAGAGGAGAG CTCCCTAGCC TCCATGCCTC GCCCACTGCG CAGCCGTGCC
    TTCTCAGAAA GCCACATCAC CCTGGATCCT CAGAGTTCCC AGGTCTGGGA GCAGCATAGG
    AAGGAGCTCT TCAACAAAGT TGATGAGACC CAGCCAGATG CTCTGGGTGC CAGGAAGAAG
    GCCTTTCCTC CTCCTCGTCC TCCTCCTCCC AACTGGGAGA AGTACAGGCT CTTCCGTGCA
    GCCCAGCAAC AGCAACAGCA GCAAAAGCAG CAGCAGCAAG AGGAGGAAGA AGAGGAGGAG
    GAGGAAGAGG AAGAAGAAGA GGAGGAAGGG GTAGAGGAGG AGGAAGAAGA GGAGGAAGCG
    GGGGAGGAGG AGGAGCTGCC ACCCCAGTAT TTCAGTTCAG AAACCACTGG TTCTGGTGCC
    CTCCATCCTG AGGAGGTTCT GGAGCAGCCC CAGCTTCCGG CCTTTGGCCA CCTGGAGGCC
    CCGAGGCAGG GCTCACAAAG CCTCCCAGCA GAGCCAGAAT CCTTTGCTCT GCGTTCCAGT
    GATTTCCTGC CTCCAGTAAG GGGCCACTTG GGATCTCAAG CTGAAAAGGC TCAACCTCCT
    TGTTATTATG GCATTGGTGG GCTTTGGAGG ACATCAGAGC AGGAGACCAC TGATTCCAAA
    CCAGAAGCTT TGATGGCGGA AGAGTCCAAA TCCAAACCAG CATGGAGCCA GAGCTACTTC
    TTTCCTGAGG AGCAGTTCTC TTTCATGGGC TGGGGCTCAG ATAAGCAGCA CCTCAACCCT
    TCAGGCTTCA GTGAACCCAA GACAACAGGA CAAGATTTTC AGCACTTCTC ACCTCCTCAG
    GGGGCCCCAG GGATCCCTAC CTCTTATTCA GCTTATTACA ATATTTCTGT GGCCAAAGCA
    GAGCTGTTGA ACAAGTTGAA AGACCAACCT GAGATGTTAG AGATTGGCCT AGGAGAGGAG
    GAAATTGACC ACGAACTGGC TCAAAAAAAG GTACAGCTTA TTGACAGCAT TGGCAGAAAA
    CTCTCTGTCC TGCGGGAGGC CCAGCGAGGA CTGCTGGAAG ACATCACTGC CAATTCTGCC
    CTTGGGGAGG AGGTGGAGGC CAACCTAAAA GCCCTCTGCA AATCCAATGA GTTTGAAAAA
    TACCGTTTAT TTATCGGGGA CCTGGACAAA GTAGTCAACC TGTTGCTGTC ACTCTCTGGT
    CGGCTGGCAC GGGTGGAGAA TGCTCTCAAC AGCATCGATT CAGAGGCCAA CCAGGAGAAG
    TTGGTGCTGA TAGAGAAGAA GCAGCAGCTG ACAGGGCAGT TGGCAGATGC CAAGGAGCTG
    AAGGAGCATG TGGACCGTCG GGAGAAGTTG GTGTTTGGCA TGGTCTCCCG TTACCTGCCT
    CAGGACCAGC TCCAAGATTA CCAGCACTTT GTGAAGATGA AATCTGCTCT CATCATTGAA
    CAGCGAGAGC TGGAAGAGAA GATCAAGCTC GGGGAAGAAC AGCTCAAATG TCTCAGGGAA
    AGCCTGCTCC TGGGGCCCAG TAATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49995
    Clone ID Related Accession (Same CDS sequence) XM_010821952.2 , XM_015470470.1 , XM_010821952.3
    Accession Version XM_015470470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
    GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGCAGCA CTGAGAAAAG CAGCTCCATT
    GGCAGTATGG AGAGCCTGGA GCAACCAGGC CAAGCCACCT ATGAGGGCCA TCTTTTGCCC
    ATTGACCAGA GCACATACCC CAACCAGCGT GATTCAGCCT ATAGCTCCTT CTCAGCCAGC
    TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGTGGACTGT
    ATCATGCAAG GCCCAGGGCC AACTAAAGCC CCTAATAGTC GCCCTAATGT GGTCGAGACC
    TCAGGAGGTG GCTGGCGCAC CAGTGGGGGT CACCTGACTG CTAGCTCCCA GATGTCATCC
    TGTCCTCAGG AGGTCTCCCA CTCAGAGCCT GCCAAGGCTG CCAGGGGTCC CCCTCAACCT
    CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAAC TCTTCAATGG AGAGCAGTGC
    AGGGCTTCTG AGCTTATAGA TTCCTTGCAA CAGAAGGAGA AACCTAGCTT GGACACCATG
    CTATCCCCAA GGAACCCTAA TAGGTTCTGC TGCCTCAGCG GGCAAGACCA AGTGACAAAT
    GAAGGCCATC AGAACTGTGA GCTCAGCCAA CCTCCTGAAT CTAACCAGAA GGGCTCTGAG
    AATCTACCGA TGGAGGCCTC AGCCAAAGCT GTTGGAGTCC CAAAAGCATG TGACAAAACT
    TCCAGCATAG GTTCCAGCCC ACTCAACCAG GCTTCAGCAG AGCTAGCTAA GACTTCTTCT
    TTTGGCAGCT CTCCACACCT CACAGGACCC ACAGGGCATC GCCATAGTGC CCCTGAACAG
    CTGCTGGTGT CCCACTTGCA GCGTGTGCAC CTTGATACTA GGGACAGCAG GGGGACAGAG
    CTCCAAGCTG GGCAGGATGG GCAGGAGTGG ACCGTATCCC CTTTGCACAT GCACAGTAGC
    CACACAAGCA AGAAAAGCCC ATGTGCCCCT GCAGGAGGAA CCCAGGACCA GCCGAGCAAA
    GACAGAAAGA CCAAGCCAGT GGATGATAGA CCTCTGGGTT CAGGTCATCA GAGCCCAAGC
    AGTTCCCCAC ATGGAGAGGC TGACGGACAC CATCCAGAAA AAGGCTTCCA GGATCCAAAC
    AGAGCAAGCA GAGCTGATAG TGAACTGGTC AGCCAGCAGT CCTCTGGCTC CCTTGCTCAA
    CAAACCAGGG ACTGTTCTTC AACCTCTAAA GTAGCTGGCA GCACAGAGGC AACTGAGGAA
    GGGGACAGTG AGCCCAAGGA GTGTGGCCGG GTGGGCAGTA AGCGAAATGG AGGGTTCCGG
    GGCCGCTCGA TCCAAAACCG GCGGAAGAGC GAACGTTTTG CTACCAATCT ACGTAATGAA
    ATTCAGAGGA GGAAGGCCCA GCTCCAGAAA GGCAAGAGTC CCTTGTCACA GCTGTGTTAC
    ACCAAGGAGC CAGTGGAAGA GACCGAGGAG ACCCTGGAAA GTCCTCCACT TCCTTCCTCT
    AACTCAGCCC TTCTATCTTC ATATAAAAAA CCACCAAGCC CCAGAGACAA GCTCTTCAAC
    AAGAGCATGA TACTCAGGGC TAGGTCTTCA GAGTGCCTCA GTCAAGCCCC TGAGGGTCAA
    GAATCTAGGA TTGGCTTGGA GGGACGAGTG AGTCCTGGCC AGAGATCTGG CCAGTCCTCT
    TTGAGCCCAA ACACCTGGTG GAAAGCATCT GACCCATCCT CGTCAGACTC TGAAAAAACA
    AACGTTCACC ATGGAGTCTG TGGAGGTCAC TGGAGATGGT CACCAGAGCA CAACCTGCAG
    CCACATGTGG CACTAGCCAT GGAAGGCCCT TCCAACCCAG GTGACAAGGA ATTGAAAGCG
    TCTACTGTCC AAGCTGGGGA GGAAGCCATC CTCTTGCCCT TTGCAGACAG AAGAAAGTTC
    TTTGAAGAGA GTAGCAAATC CTTATCTACA TCTCATTTGC CAGGTTTAAC CACTCATAAC
    AAGACTTTTA CCCAGAGACC AAAACCTATT GACCAAAGCT TTCAGCCAAT GAGCTCCAGC
    TATAGAGAAT TGAGGCGCCA TCCCATAGAC CAATCATATC ACTCCACAGA CCAACCCTAT
    CATGCCACAG ACCAATCATA TCATTCCATA TCACCCCTTC AGTCAGAAAC TCCCACTTAC
    ACAGAATGTT TTGCAAGCAA AGGTCTAGAA CAATCCATGT GCTGTAAGCC ACTACACTGT
    GGTGATTTTG ATTACCACAG GACGTGCTCT TACTCCTGCA ATGTCCAGGG AGCTGTAGTC
    CATGACCCTT GCATTTATTG TTCTGGGGAA ATCTGTCCTG CTTTGCGAAA GAGAAATATG
    ATTCCAAACT GCTACAATTG CCGGTGCCAC CATCACCAGT GCATCCGGTG TTCAGCTTGC
    TGTCATAATC CCCAGCATGG TACCCTCGAG GACAGCAGCT TGAAACCTGG CAACACTTGG
    AAACCCAGGA AGCCGACAGT GCAGGAATTT CCTGGGGACA AATGGAAACC AATAACAGGA
    AACAGGAAGA CCAGCCAGTC AGGGAGGGAA ATGGCTCATT CCAAGGCTAG CTTTTCATGG
    GCAACCCCAT TCCATCCTTG CCTTGAGAAC CCAGCACTGG ACTTGTCAAA CTACCGAGCA
    GTCTCTTCTC TTGACCTCCT TGGAGACTTT AAGCACTCCT CCAAAAAAAC AGAGGAAACC
    TCCGTTTATG AAGAGGAGAG CTCCCTAGCC TCCATGCCTC GCCCACTGCG CAGCCGTGCC
    TTCTCAGAAA GCCACATCAC CCTGGATCCT CAGAGTTCCC AGGTCTGGGA GCAGCATAGG
    AAGGAGCTCT TCAACAAAGT TGATGAGACC CAGCCAGATG CTCTGGGTGC CAGGAAGAAG
    GCCTTTCCTC CTCCTCGTCC TCCTCCTCCC AACTGGGAGA AGTACAGGCT CTTCCGTGCA
    GCCCAGCAAC AGCAACAGCA GCAAAAGCAG CAGCAGCAAG AGGAGGAAGA AGAGGAGGAG
    GAGGAAGAGG AAGAAGAAGA GGAGGAAGGG GTAGAGGAGG AGGAAGAAGA GGAGGAAGCG
    GGGGAGGAGG AGGAGCTGCC ACCCCAGTAT TTCAGTTCAG AAACCACTGG TTCTGGTGCC
    CTCCATCCTG AGGAGGTTCT GGAGCAGCCC CAGCTTCCGG CCTTTGGCCA CCTGGAGGCC
    CCGAGGCAGG GCTCACAAAG CCTCCCAGCA GAGCCAGAAT CCTTTGCTCT GCGTTCCAGT
    GATTTCCTGC CTCCAGTAAG GGGCCACTTG GGATCTCAAG CTGAAAAGGC TCAACCTCCT
    TGTTATTATG GCATTGGTGG GCTTTGGAGG ACATCAGAGC AGGAGACCAC TGATTCCAAA
    CCAGAAGCTT TGATGGCGGA AGAGTCCAAA TCCAAACCAG CATGGAGCCA GAGCTACTTC
    TTTCCTGAGG AGCAGTTCTC TTTCATGGGC TGGGGCTCAG ATAAGCAGCA CCTCAACCCT
    TCAGGCTTCA GTGAACCCAA GACAACAGGA CAAGATTTTC AGCACTTCTC ACCTCCTCAG
    GGGGCCCCAG GGATCCCTAC CTCTTATTCA GCTTATTACA ATATTTCTGT GGCCAAAGCA
    GAGCTGTTGA ACAAGTTGAA AGACCAACCT GAGATGTTAG AGATTGGCCT AGGAGAGGAG
    GAAATTGACC ACGAACTGGC TCAAAAAAAG GTACAGCTTA TTGACAGCAT TGGCAGAAAA
    CTCTCTGTCC TGCGGGAGGC CCAGCGAGGA CTGCTGGAAG ACATCACTGC CAATTCTGCC
    CTTGGGGAGG AGGTGGAGGC CAACCTAAAA GCCCTCTGCA AATCCAATGA GTTTGAAAAA
    TACCGTTTAT TTATCGGGGA CCTGGACAAA GTAGTCAACC TGTTGCTGTC ACTCTCTGGT
    CGGCTGGCAC GGGTGGAGAA TGCTCTCAAC AGCATCGATT CAGAGGCCAA CCAGGAGAAG
    TTGGTGCTGA TAGAGAAGAA GCAGCAGCTG ACAGGGCAGT TGGCAGATGC CAAGGAGCTG
    AAGGAGCATG TGGACCGTCG GGAGAAGTTG GTGTTTGGCA TGGTCTCCCG TTACCTGCCT
    CAGGACCAGC TCCAAGATTA CCAGCACTTT GTGAAGATGA AATCTGCTCT CATCATTGAA
    CAGCGAGAGC TGGAAGAGAA GATCAAGCTC GGGGAAGAAC AGCTCAAATG TCTCAGGGAA
    AGCCTGCTCC TGGGGCCCAG TAATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325956.1
    CDS538..4827
    Translation

    Target ORF information:

    RefSeq Version XM_015470470.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
    GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGCAGCA CTGAGAAAAG CAGCTCCATT
    GGCAGTATGG AGAGCCTGGA GCAACCAGGC CAAGCCACCT ATGAGGGCCA TCTTTTGCCC
    ATTGACCAGA GCACATACCC CAACCAGCGT GATTCAGCCT ATAGCTCCTT CTCAGCCAGC
    TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGTGGACTGT
    ATCATGCAAG GCCCAGGGCC AACTAAAGCC CCTAATAGTC GCCCTAATGT GGTCGAGACC
    TCAGGAGGTG GCTGGCGCAC CAGTGGGGGT CACCTGACTG CTAGCTCCCA GATGTCATCC
    TGTCCTCAGG AGGTCTCCCA CTCAGAGCCT GCCAAGGCTG CCAGGGGTCC CCCTCAACCT
    CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAAC TCTTCAATGG AGAGCAGTGC
    AGGGCTTCTG AGCTTATAGA TTCCTTGCAA CAGAAGGAGA AACCTAGCTT GGACACCATG
    CTATCCCCAA GGAACCCTAA TAGGTTCTGC TGCCTCAGCG GGCAAGACCA AGTGACAAAT
    GAAGGCCATC AGAACTGTGA GCTCAGCCAA CCTCCTGAAT CTAACCAGAA GGGCTCTGAG
    AATCTACCGA TGGAGGCCTC AGCCAAAGCT GTTGGAGTCC CAAAAGCATG TGACAAAACT
    TCCAGCATAG GTTCCAGCCC ACTCAACCAG GCTTCAGCAG AGCTAGCTAA GACTTCTTCT
    TTTGGCAGCT CTCCACACCT CACAGGACCC ACAGGGCATC GCCATAGTGC CCCTGAACAG
    CTGCTGGTGT CCCACTTGCA GCGTGTGCAC CTTGATACTA GGGACAGCAG GGGGACAGAG
    CTCCAAGCTG GGCAGGATGG GCAGGAGTGG ACCGTATCCC CTTTGCACAT GCACAGTAGC
    CACACAAGCA AGAAAAGCCC ATGTGCCCCT GCAGGAGGAA CCCAGGACCA GCCGAGCAAA
    GACAGAAAGA CCAAGCCAGT GGATGATAGA CCTCTGGGTT CAGGTCATCA GAGCCCAAGC
    AGTTCCCCAC ATGGAGAGGC TGACGGACAC CATCCAGAAA AAGGCTTCCA GGATCCAAAC
    AGAGCAAGCA GAGCTGATAG TGAACTGGTC AGCCAGCAGT CCTCTGGCTC CCTTGCTCAA
    CAAACCAGGG ACTGTTCTTC AACCTCTAAA GTAGCTGGCA GCACAGAGGC AACTGAGGAA
    GGGGACAGTG AGCCCAAGGA GTGTGGCCGG GTGGGCAGTA AGCGAAATGG AGGGTTCCGG
    GGCCGCTCGA TCCAAAACCG GCGGAAGAGC GAACGTTTTG CTACCAATCT ACGTAATGAA
    ATTCAGAGGA GGAAGGCCCA GCTCCAGAAA GGCAAGAGTC CCTTGTCACA GCTGTGTTAC
    ACCAAGGAGC CAGTGGAAGA GACCGAGGAG ACCCTGGAAA GTCCTCCACT TCCTTCCTCT
    AACTCAGCCC TTCTATCTTC ATATAAAAAA CCACCAAGCC CCAGAGACAA GCTCTTCAAC
    AAGAGCATGA TACTCAGGGC TAGGTCTTCA GAGTGCCTCA GTCAAGCCCC TGAGGGTCAA
    GAATCTAGGA TTGGCTTGGA GGGACGAGTG AGTCCTGGCC AGAGATCTGG CCAGTCCTCT
    TTGAGCCCAA ACACCTGGTG GAAAGCATCT GACCCATCCT CGTCAGACTC TGAAAAAACA
    AACGTTCACC ATGGAGTCTG TGGAGGTCAC TGGAGATGGT CACCAGAGCA CAACCTGCAG
    CCACATGTGG CACTAGCCAT GGAAGGCCCT TCCAACCCAG GTGACAAGGA ATTGAAAGCG
    TCTACTGTCC AAGCTGGGGA GGAAGCCATC CTCTTGCCCT TTGCAGACAG AAGAAAGTTC
    TTTGAAGAGA GTAGCAAATC CTTATCTACA TCTCATTTGC CAGGTTTAAC CACTCATAAC
    AAGACTTTTA CCCAGAGACC AAAACCTATT GACCAAAGCT TTCAGCCAAT GAGCTCCAGC
    TATAGAGAAT TGAGGCGCCA TCCCATAGAC CAATCATATC ACTCCACAGA CCAACCCTAT
    CATGCCACAG ACCAATCATA TCATTCCATA TCACCCCTTC AGTCAGAAAC TCCCACTTAC
    ACAGAATGTT TTGCAAGCAA AGGTCTAGAA CAATCCATGT GCTGTAAGCC ACTACACTGT
    GGTGATTTTG ATTACCACAG GACGTGCTCT TACTCCTGCA ATGTCCAGGG AGCTGTAGTC
    CATGACCCTT GCATTTATTG TTCTGGGGAA ATCTGTCCTG CTTTGCGAAA GAGAAATATG
    ATTCCAAACT GCTACAATTG CCGGTGCCAC CATCACCAGT GCATCCGGTG TTCAGCTTGC
    TGTCATAATC CCCAGCATGG TACCCTCGAG GACAGCAGCT TGAAACCTGG CAACACTTGG
    AAACCCAGGA AGCCGACAGT GCAGGAATTT CCTGGGGACA AATGGAAACC AATAACAGGA
    AACAGGAAGA CCAGCCAGTC AGGGAGGGAA ATGGCTCATT CCAAGGCTAG CTTTTCATGG
    GCAACCCCAT TCCATCCTTG CCTTGAGAAC CCAGCACTGG ACTTGTCAAA CTACCGAGCA
    GTCTCTTCTC TTGACCTCCT TGGAGACTTT AAGCACTCCT CCAAAAAAAC AGAGGAAACC
    TCCGTTTATG AAGAGGAGAG CTCCCTAGCC TCCATGCCTC GCCCACTGCG CAGCCGTGCC
    TTCTCAGAAA GCCACATCAC CCTGGATCCT CAGAGTTCCC AGGTCTGGGA GCAGCATAGG
    AAGGAGCTCT TCAACAAAGT TGATGAGACC CAGCCAGATG CTCTGGGTGC CAGGAAGAAG
    GCCTTTCCTC CTCCTCGTCC TCCTCCTCCC AACTGGGAGA AGTACAGGCT CTTCCGTGCA
    GCCCAGCAAC AGCAACAGCA GCAAAAGCAG CAGCAGCAAG AGGAGGAAGA AGAGGAGGAG
    GAGGAAGAGG AAGAAGAAGA GGAGGAAGGG GTAGAGGAGG AGGAAGAAGA GGAGGAAGCG
    GGGGAGGAGG AGGAGCTGCC ACCCCAGTAT TTCAGTTCAG AAACCACTGG TTCTGGTGCC
    CTCCATCCTG AGGAGGTTCT GGAGCAGCCC CAGCTTCCGG CCTTTGGCCA CCTGGAGGCC
    CCGAGGCAGG GCTCACAAAG CCTCCCAGCA GAGCCAGAAT CCTTTGCTCT GCGTTCCAGT
    GATTTCCTGC CTCCAGTAAG GGGCCACTTG GGATCTCAAG CTGAAAAGGC TCAACCTCCT
    TGTTATTATG GCATTGGTGG GCTTTGGAGG ACATCAGAGC AGGAGACCAC TGATTCCAAA
    CCAGAAGCTT TGATGGCGGA AGAGTCCAAA TCCAAACCAG CATGGAGCCA GAGCTACTTC
    TTTCCTGAGG AGCAGTTCTC TTTCATGGGC TGGGGCTCAG ATAAGCAGCA CCTCAACCCT
    TCAGGCTTCA GTGAACCCAA GACAACAGGA CAAGATTTTC AGCACTTCTC ACCTCCTCAG
    GGGGCCCCAG GGATCCCTAC CTCTTATTCA GCTTATTACA ATATTTCTGT GGCCAAAGCA
    GAGCTGTTGA ACAAGTTGAA AGACCAACCT GAGATGTTAG AGATTGGCCT AGGAGAGGAG
    GAAATTGACC ACGAACTGGC TCAAAAAAAG GTACAGCTTA TTGACAGCAT TGGCAGAAAA
    CTCTCTGTCC TGCGGGAGGC CCAGCGAGGA CTGCTGGAAG ACATCACTGC CAATTCTGCC
    CTTGGGGAGG AGGTGGAGGC CAACCTAAAA GCCCTCTGCA AATCCAATGA GTTTGAAAAA
    TACCGTTTAT TTATCGGGGA CCTGGACAAA GTAGTCAACC TGTTGCTGTC ACTCTCTGGT
    CGGCTGGCAC GGGTGGAGAA TGCTCTCAAC AGCATCGATT CAGAGGCCAA CCAGGAGAAG
    TTGGTGCTGA TAGAGAAGAA GCAGCAGCTG ACAGGGCAGT TGGCAGATGC CAAGGAGCTG
    AAGGAGCATG TGGACCGTCG GGAGAAGTTG GTGTTTGGCA TGGTCTCCCG TTACCTGCCT
    CAGGACCAGC TCCAAGATTA CCAGCACTTT GTGAAGATGA AATCTGCTCT CATCATTGAA
    CAGCGAGAGC TGGAAGAGAA GATCAAGCTC GGGGAAGAAC AGCTCAAATG TCTCAGGGAA
    AGCCTGCTCC TGGGGCCCAG TAATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49995
    Clone ID Related Accession (Same CDS sequence) XM_010821952.2 , XM_015470470.1 , XM_010821952.3
    Accession Version XM_010821952.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
    GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGCAGCA CTGAGAAAAG CAGCTCCATT
    GGCAGTATGG AGAGCCTGGA GCAACCAGGC CAAGCCACCT ATGAGGGCCA TCTTTTGCCC
    ATTGACCAGA GCACATACCC CAACCAGCGT GATTCAGCCT ATAGCTCCTT CTCAGCCAGC
    TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGTGGACTGT
    ATCATGCAAG GCCCAGGGCC AACTAAAGCC CCTAATAGTC GCCCTAATGT GGTCGAGACC
    TCAGGAGGTG GCTGGCGCAC CAGTGGGGGT CACCTGACTG CTAGCTCCCA GATGTCATCC
    TGTCCTCAGG AGGTCTCCCA CTCAGAGCCT GCCAAGGCTG CCAGGGGTCC CCCTCAACCT
    CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAAC TCTTCAATGG AGAGCAGTGC
    AGGGCTTCTG AGCTTATAGA TTCCTTGCAA CAGAAGGAGA AACCTAGCTT GGACACCATG
    CTATCCCCAA GGAACCCTAA TAGGTTCTGC TGCCTCAGCG GGCAAGACCA AGTGACAAAT
    GAAGGCCATC AGAACTGTGA GCTCAGCCAA CCTCCTGAAT CTAACCAGAA GGGCTCTGAG
    AATCTACCGA TGGAGGCCTC AGCCAAAGCT GTTGGAGTCC CAAAAGCATG TGACAAAACT
    TCCAGCATAG GTTCCAGCCC ACTCAACCAG GCTTCAGCAG AGCTAGCTAA GACTTCTTCT
    TTTGGCAGCT CTCCACACCT CACAGGACCC ACAGGGCATC GCCATAGTGC CCCTGAACAG
    CTGCTGGTGT CCCACTTGCA GCGTGTGCAC CTTGATACTA GGGACAGCAG GGGGACAGAG
    CTCCAAGCTG GGCAGGATGG GCAGGAGTGG ACCGTATCCC CTTTGCACAT GCACAGTAGC
    CACACAAGCA AGAAAAGCCC ATGTGCCCCT GCAGGAGGAA CCCAGGACCA GCCGAGCAAA
    GACAGAAAGA CCAAGCCAGT GGATGATAGA CCTCTGGGTT CAGGTCATCA GAGCCCAAGC
    AGTTCCCCAC ATGGAGAGGC TGACGGACAC CATCCAGAAA AAGGCTTCCA GGATCCAAAC
    AGAGCAAGCA GAGCTGATAG TGAACTGGTC AGCCAGCAGT CCTCTGGCTC CCTTGCTCAA
    CAAACCAGGG ACTGTTCTTC AACCTCTAAA GTAGCTGGCA GCACAGAGGC AACTGAGGAA
    GGGGACAGTG AGCCCAAGGA GTGTGGCCGG GTGGGCAGTA AGCGAAATGG AGGGTTCCGG
    GGCCGCTCGA TCCAAAACCG GCGGAAGAGC GAACGTTTTG CTACCAATCT ACGTAATGAA
    ATTCAGAGGA GGAAGGCCCA GCTCCAGAAA GGCAAGAGTC CCTTGTCACA GCTGTGTTAC
    ACCAAGGAGC CAGTGGAAGA GACCGAGGAG ACCCTGGAAA GTCCTCCACT TCCTTCCTCT
    AACTCAGCCC TTCTATCTTC ATATAAAAAA CCACCAAGCC CCAGAGACAA GCTCTTCAAC
    AAGAGCATGA TACTCAGGGC TAGGTCTTCA GAGTGCCTCA GTCAAGCCCC TGAGGGTCAA
    GAATCTAGGA TTGGCTTGGA GGGACGAGTG AGTCCTGGCC AGAGATCTGG CCAGTCCTCT
    TTGAGCCCAA ACACCTGGTG GAAAGCATCT GACCCATCCT CGTCAGACTC TGAAAAAACA
    AACGTTCACC ATGGAGTCTG TGGAGGTCAC TGGAGATGGT CACCAGAGCA CAACCTGCAG
    CCACATGTGG CACTAGCCAT GGAAGGCCCT TCCAACCCAG GTGACAAGGA ATTGAAAGCG
    TCTACTGTCC AAGCTGGGGA GGAAGCCATC CTCTTGCCCT TTGCAGACAG AAGAAAGTTC
    TTTGAAGAGA GTAGCAAATC CTTATCTACA TCTCATTTGC CAGGTTTAAC CACTCATAAC
    AAGACTTTTA CCCAGAGACC AAAACCTATT GACCAAAGCT TTCAGCCAAT GAGCTCCAGC
    TATAGAGAAT TGAGGCGCCA TCCCATAGAC CAATCATATC ACTCCACAGA CCAACCCTAT
    CATGCCACAG ACCAATCATA TCATTCCATA TCACCCCTTC AGTCAGAAAC TCCCACTTAC
    ACAGAATGTT TTGCAAGCAA AGGTCTAGAA CAATCCATGT GCTGTAAGCC ACTACACTGT
    GGTGATTTTG ATTACCACAG GACGTGCTCT TACTCCTGCA ATGTCCAGGG AGCTGTAGTC
    CATGACCCTT GCATTTATTG TTCTGGGGAA ATCTGTCCTG CTTTGCGAAA GAGAAATATG
    ATTCCAAACT GCTACAATTG CCGGTGCCAC CATCACCAGT GCATCCGGTG TTCAGCTTGC
    TGTCATAATC CCCAGCATGG TACCCTCGAG GACAGCAGCT TGAAACCTGG CAACACTTGG
    AAACCCAGGA AGCCGACAGT GCAGGAATTT CCTGGGGACA AATGGAAACC AATAACAGGA
    AACAGGAAGA CCAGCCAGTC AGGGAGGGAA ATGGCTCATT CCAAGGCTAG CTTTTCATGG
    GCAACCCCAT TCCATCCTTG CCTTGAGAAC CCAGCACTGG ACTTGTCAAA CTACCGAGCA
    GTCTCTTCTC TTGACCTCCT TGGAGACTTT AAGCACTCCT CCAAAAAAAC AGAGGAAACC
    TCCGTTTATG AAGAGGAGAG CTCCCTAGCC TCCATGCCTC GCCCACTGCG CAGCCGTGCC
    TTCTCAGAAA GCCACATCAC CCTGGATCCT CAGAGTTCCC AGGTCTGGGA GCAGCATAGG
    AAGGAGCTCT TCAACAAAGT TGATGAGACC CAGCCAGATG CTCTGGGTGC CAGGAAGAAG
    GCCTTTCCTC CTCCTCGTCC TCCTCCTCCC AACTGGGAGA AGTACAGGCT CTTCCGTGCA
    GCCCAGCAAC AGCAACAGCA GCAAAAGCAG CAGCAGCAAG AGGAGGAAGA AGAGGAGGAG
    GAGGAAGAGG AAGAAGAAGA GGAGGAAGGG GTAGAGGAGG AGGAAGAAGA GGAGGAAGCG
    GGGGAGGAGG AGGAGCTGCC ACCCCAGTAT TTCAGTTCAG AAACCACTGG TTCTGGTGCC
    CTCCATCCTG AGGAGGTTCT GGAGCAGCCC CAGCTTCCGG CCTTTGGCCA CCTGGAGGCC
    CCGAGGCAGG GCTCACAAAG CCTCCCAGCA GAGCCAGAAT CCTTTGCTCT GCGTTCCAGT
    GATTTCCTGC CTCCAGTAAG GGGCCACTTG GGATCTCAAG CTGAAAAGGC TCAACCTCCT
    TGTTATTATG GCATTGGTGG GCTTTGGAGG ACATCAGAGC AGGAGACCAC TGATTCCAAA
    CCAGAAGCTT TGATGGCGGA AGAGTCCAAA TCCAAACCAG CATGGAGCCA GAGCTACTTC
    TTTCCTGAGG AGCAGTTCTC TTTCATGGGC TGGGGCTCAG ATAAGCAGCA CCTCAACCCT
    TCAGGCTTCA GTGAACCCAA GACAACAGGA CAAGATTTTC AGCACTTCTC ACCTCCTCAG
    GGGGCCCCAG GGATCCCTAC CTCTTATTCA GCTTATTACA ATATTTCTGT GGCCAAAGCA
    GAGCTGTTGA ACAAGTTGAA AGACCAACCT GAGATGTTAG AGATTGGCCT AGGAGAGGAG
    GAAATTGACC ACGAACTGGC TCAAAAAAAG GTACAGCTTA TTGACAGCAT TGGCAGAAAA
    CTCTCTGTCC TGCGGGAGGC CCAGCGAGGA CTGCTGGAAG ACATCACTGC CAATTCTGCC
    CTTGGGGAGG AGGTGGAGGC CAACCTAAAA GCCCTCTGCA AATCCAATGA GTTTGAAAAA
    TACCGTTTAT TTATCGGGGA CCTGGACAAA GTAGTCAACC TGTTGCTGTC ACTCTCTGGT
    CGGCTGGCAC GGGTGGAGAA TGCTCTCAAC AGCATCGATT CAGAGGCCAA CCAGGAGAAG
    TTGGTGCTGA TAGAGAAGAA GCAGCAGCTG ACAGGGCAGT TGGCAGATGC CAAGGAGCTG
    AAGGAGCATG TGGACCGTCG GGAGAAGTTG GTGTTTGGCA TGGTCTCCCG TTACCTGCCT
    CAGGACCAGC TCCAAGATTA CCAGCACTTT GTGAAGATGA AATCTGCTCT CATCATTGAA
    CAGCGAGAGC TGGAAGAGAA GATCAAGCTC GGGGAAGAAC AGCTCAAATG TCTCAGGGAA
    AGCCTGCTCC TGGGGCCCAG TAATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820254.1
    CDS555..4844
    Translation

    Target ORF information:

    RefSeq Version XM_010821952.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821952.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
    GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGCAGCA CTGAGAAAAG CAGCTCCATT
    GGCAGTATGG AGAGCCTGGA GCAACCAGGC CAAGCCACCT ATGAGGGCCA TCTTTTGCCC
    ATTGACCAGA GCACATACCC CAACCAGCGT GATTCAGCCT ATAGCTCCTT CTCAGCCAGC
    TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGTGGACTGT
    ATCATGCAAG GCCCAGGGCC AACTAAAGCC CCTAATAGTC GCCCTAATGT GGTCGAGACC
    TCAGGAGGTG GCTGGCGCAC CAGTGGGGGT CACCTGACTG CTAGCTCCCA GATGTCATCC
    TGTCCTCAGG AGGTCTCCCA CTCAGAGCCT GCCAAGGCTG CCAGGGGTCC CCCTCAACCT
    CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAAC TCTTCAATGG AGAGCAGTGC
    AGGGCTTCTG AGCTTATAGA TTCCTTGCAA CAGAAGGAGA AACCTAGCTT GGACACCATG
    CTATCCCCAA GGAACCCTAA TAGGTTCTGC TGCCTCAGCG GGCAAGACCA AGTGACAAAT
    GAAGGCCATC AGAACTGTGA GCTCAGCCAA CCTCCTGAAT CTAACCAGAA GGGCTCTGAG
    AATCTACCGA TGGAGGCCTC AGCCAAAGCT GTTGGAGTCC CAAAAGCATG TGACAAAACT
    TCCAGCATAG GTTCCAGCCC ACTCAACCAG GCTTCAGCAG AGCTAGCTAA GACTTCTTCT
    TTTGGCAGCT CTCCACACCT CACAGGACCC ACAGGGCATC GCCATAGTGC CCCTGAACAG
    CTGCTGGTGT CCCACTTGCA GCGTGTGCAC CTTGATACTA GGGACAGCAG GGGGACAGAG
    CTCCAAGCTG GGCAGGATGG GCAGGAGTGG ACCGTATCCC CTTTGCACAT GCACAGTAGC
    CACACAAGCA AGAAAAGCCC ATGTGCCCCT GCAGGAGGAA CCCAGGACCA GCCGAGCAAA
    GACAGAAAGA CCAAGCCAGT GGATGATAGA CCTCTGGGTT CAGGTCATCA GAGCCCAAGC
    AGTTCCCCAC ATGGAGAGGC TGACGGACAC CATCCAGAAA AAGGCTTCCA GGATCCAAAC
    AGAGCAAGCA GAGCTGATAG TGAACTGGTC AGCCAGCAGT CCTCTGGCTC CCTTGCTCAA
    CAAACCAGGG ACTGTTCTTC AACCTCTAAA GTAGCTGGCA GCACAGAGGC AACTGAGGAA
    GGGGACAGTG AGCCCAAGGA GTGTGGCCGG GTGGGCAGTA AGCGAAATGG AGGGTTCCGG
    GGCCGCTCGA TCCAAAACCG GCGGAAGAGC GAACGTTTTG CTACCAATCT ACGTAATGAA
    ATTCAGAGGA GGAAGGCCCA GCTCCAGAAA GGCAAGAGTC CCTTGTCACA GCTGTGTTAC
    ACCAAGGAGC CAGTGGAAGA GACCGAGGAG ACCCTGGAAA GTCCTCCACT TCCTTCCTCT
    AACTCAGCCC TTCTATCTTC ATATAAAAAA CCACCAAGCC CCAGAGACAA GCTCTTCAAC
    AAGAGCATGA TACTCAGGGC TAGGTCTTCA GAGTGCCTCA GTCAAGCCCC TGAGGGTCAA
    GAATCTAGGA TTGGCTTGGA GGGACGAGTG AGTCCTGGCC AGAGATCTGG CCAGTCCTCT
    TTGAGCCCAA ACACCTGGTG GAAAGCATCT GACCCATCCT CGTCAGACTC TGAAAAAACA
    AACGTTCACC ATGGAGTCTG TGGAGGTCAC TGGAGATGGT CACCAGAGCA CAACCTGCAG
    CCACATGTGG CACTAGCCAT GGAAGGCCCT TCCAACCCAG GTGACAAGGA ATTGAAAGCG
    TCTACTGTCC AAGCTGGGGA GGAAGCCATC CTCTTGCCCT TTGCAGACAG AAGAAAGTTC
    TTTGAAGAGA GTAGCAAATC CTTATCTACA TCTCATTTGC CAGGTTTAAC CACTCATAAC
    AAGACTTTTA CCCAGAGACC AAAACCTATT GACCAAAGCT TTCAGCCAAT GAGCTCCAGC
    TATAGAGAAT TGAGGCGCCA TCCCATAGAC CAATCATATC ACTCCACAGA CCAACCCTAT
    CATGCCACAG ACCAATCATA TCATTCCATA TCACCCCTTC AGTCAGAAAC TCCCACTTAC
    ACAGAATGTT TTGCAAGCAA AGGTCTAGAA CAATCCATGT GCTGTAAGCC ACTACACTGT
    GGTGATTTTG ATTACCACAG GACGTGCTCT TACTCCTGCA ATGTCCAGGG AGCTGTAGTC
    CATGACCCTT GCATTTATTG TTCTGGGGAA ATCTGTCCTG CTTTGCGAAA GAGAAATATG
    ATTCCAAACT GCTACAATTG CCGGTGCCAC CATCACCAGT GCATCCGGTG TTCAGCTTGC
    TGTCATAATC CCCAGCATGG TACCCTCGAG GACAGCAGCT TGAAACCTGG CAACACTTGG
    AAACCCAGGA AGCCGACAGT GCAGGAATTT CCTGGGGACA AATGGAAACC AATAACAGGA
    AACAGGAAGA CCAGCCAGTC AGGGAGGGAA ATGGCTCATT CCAAGGCTAG CTTTTCATGG
    GCAACCCCAT TCCATCCTTG CCTTGAGAAC CCAGCACTGG ACTTGTCAAA CTACCGAGCA
    GTCTCTTCTC TTGACCTCCT TGGAGACTTT AAGCACTCCT CCAAAAAAAC AGAGGAAACC
    TCCGTTTATG AAGAGGAGAG CTCCCTAGCC TCCATGCCTC GCCCACTGCG CAGCCGTGCC
    TTCTCAGAAA GCCACATCAC CCTGGATCCT CAGAGTTCCC AGGTCTGGGA GCAGCATAGG
    AAGGAGCTCT TCAACAAAGT TGATGAGACC CAGCCAGATG CTCTGGGTGC CAGGAAGAAG
    GCCTTTCCTC CTCCTCGTCC TCCTCCTCCC AACTGGGAGA AGTACAGGCT CTTCCGTGCA
    GCCCAGCAAC AGCAACAGCA GCAAAAGCAG CAGCAGCAAG AGGAGGAAGA AGAGGAGGAG
    GAGGAAGAGG AAGAAGAAGA GGAGGAAGGG GTAGAGGAGG AGGAAGAAGA GGAGGAAGCG
    GGGGAGGAGG AGGAGCTGCC ACCCCAGTAT TTCAGTTCAG AAACCACTGG TTCTGGTGCC
    CTCCATCCTG AGGAGGTTCT GGAGCAGCCC CAGCTTCCGG CCTTTGGCCA CCTGGAGGCC
    CCGAGGCAGG GCTCACAAAG CCTCCCAGCA GAGCCAGAAT CCTTTGCTCT GCGTTCCAGT
    GATTTCCTGC CTCCAGTAAG GGGCCACTTG GGATCTCAAG CTGAAAAGGC TCAACCTCCT
    TGTTATTATG GCATTGGTGG GCTTTGGAGG ACATCAGAGC AGGAGACCAC TGATTCCAAA
    CCAGAAGCTT TGATGGCGGA AGAGTCCAAA TCCAAACCAG CATGGAGCCA GAGCTACTTC
    TTTCCTGAGG AGCAGTTCTC TTTCATGGGC TGGGGCTCAG ATAAGCAGCA CCTCAACCCT
    TCAGGCTTCA GTGAACCCAA GACAACAGGA CAAGATTTTC AGCACTTCTC ACCTCCTCAG
    GGGGCCCCAG GGATCCCTAC CTCTTATTCA GCTTATTACA ATATTTCTGT GGCCAAAGCA
    GAGCTGTTGA ACAAGTTGAA AGACCAACCT GAGATGTTAG AGATTGGCCT AGGAGAGGAG
    GAAATTGACC ACGAACTGGC TCAAAAAAAG GTACAGCTTA TTGACAGCAT TGGCAGAAAA
    CTCTCTGTCC TGCGGGAGGC CCAGCGAGGA CTGCTGGAAG ACATCACTGC CAATTCTGCC
    CTTGGGGAGG AGGTGGAGGC CAACCTAAAA GCCCTCTGCA AATCCAATGA GTTTGAAAAA
    TACCGTTTAT TTATCGGGGA CCTGGACAAA GTAGTCAACC TGTTGCTGTC ACTCTCTGGT
    CGGCTGGCAC GGGTGGAGAA TGCTCTCAAC AGCATCGATT CAGAGGCCAA CCAGGAGAAG
    TTGGTGCTGA TAGAGAAGAA GCAGCAGCTG ACAGGGCAGT TGGCAGATGC CAAGGAGCTG
    AAGGAGCATG TGGACCGTCG GGAGAAGTTG GTGTTTGGCA TGGTCTCCCG TTACCTGCCT
    CAGGACCAGC TCCAAGATTA CCAGCACTTT GTGAAGATGA AATCTGCTCT CATCATTGAA
    CAGCGAGAGC TGGAAGAGAA GATCAAGCTC GGGGAAGAAC AGCTCAAATG TCTCAGGGAA
    AGCCTGCTCC TGGGGCCCAG TAATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89376
    Clone ID Related Accession (Same CDS sequence) XM_015470469.1 , XM_015461686.2 , XM_015461686.1
    Accession Version XM_015470469.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTACGG CTCCCGCCAA 
    GAGGCTCTCA TCCTCATCAA AGGCTCCTTC CGGACCCTCA AGCTGATTGT CAGGAGGAGA
    AACGCCCCTG TCAGTAGGCC GCACTCATGG CATGTGGCCA AGCTGCTGGA GGGATGCCCT
    GAAGCGGCCA CCACCATGCA TTTCCCTTCT GAAGCCTTCA GCTTGTCCTG GCATTCCGGC
    TGCAACACAA GTGACGTGTG TGTGCAGTGG TGTCCACTCT CCCGGCACTG CAGCACTGAG
    AAAAGCAGCT CCATTGGCAG TATGGAGAGC CTGGAGCAAC CAGGCCAAGC CACCTATGAG
    GGCCATCTTT TGCCCATTGA CCAGAGCACA TACCCCAACC AGCGTGATTC AGCCTATAGC
    TCCTTCTCAG CCAGCTCAAA TGCCTCTGAC TGTGCCCTTT CCCTCAGGCC AGAGGAGCCA
    GCCTCTGTGG ACTGTATCAT GCAAGGCCCA GGGCCAACTA AAGCCCCTAA TAGTCGCCCT
    AATGTGGTCG AGACCTCAGG AGGTGGCTGG CGCACCAGTG GGGGTCACCT GACTGCTAGC
    TCCCAGATGT CATCCTGTCC TCAGGAGGTC TCCCACTCAG AGCCTGCCAA GGCTGCCAGG
    GGTCCCCCTC AACCTCCAGT GAGGCGAGAC AGTCTTCAGG CCTCCAGAGC CCAACTCTTC
    AATGGAGAGC AGTGCAGGGC TTCTGAGCTT ATAGATTCCT TGCAACAGAA GGAGAAACCT
    AGCTTGGACA CCATGCTATC CCCAAGGAAC CCTAATAGGT TCTGCTGCCT CAGCGGGCAA
    GACCAAGTGA CAAATGAAGG CCATCAGAAC TGTGAGCTCA GCCAACCTCC TGAATCTAAC
    CAGAAGGGCT CTGAGAATCT ACCGATGGAG GCCTCAGCCA AAGCTGTTGG AGTCCCAAAA
    GCATGTGACA AAACTTCCAG CATAGGTTCC AGCCCACTCA ACCAGGCTTC AGCAGAGCTA
    GCTAAGACTT CTTCTTTTGG CAGCTCTCCA CACCTCACAG GACCCACAGG GCATCGCCAT
    AGTGCCCCTG AACAGCTGCT GGTGTCCCAC TTGCAGCGTG TGCACCTTGA TACTAGGGAC
    AGCAGGGGGA CAGAGCTCCA AGCTGGGCAG GATGGGCAGG AGTGGACCGT ATCCCCTTTG
    CACATGCACA GTAGCCACAC AAGCAAGAAA AGCCCATGTG CCCCTGCAGG AGGAACCCAG
    GACCAGCCGA GCAAAGACAG AAAGACCAAG CCAGTGGATG ATAGACCTCT GGGTTCAGGT
    CATCAGAGCC CAAGCAGTTC CCCACATGGA GAGGCTGACG GACACCATCC AGAAAAAGGC
    TTCCAGGATC CAAACAGAGC AAGCAGAGCT GATAGTGAAC TGGTCAGCCA GCAGTCCTCT
    GGCTCCCTTG CTCAACAAAC CAGGGACTGT TCTTCAACCT CTAAAGTAGC TGGCAGCACA
    GAGGCAACTG AGGAAGGGGA CAGTGAGCCC AAGGAGTGTG GCCGGGTGGG CAGTAAGCGA
    AATGGAGGGT TCCGGGGCCG CTCGATCCAA AACCGGCGGA AGAGCGAACG TTTTGCTACC
    AATCTACGTA ATGAAATTCA GAGGAGGAAG GCCCAGCTCC AGAAAGGCAA GAGTCCCTTG
    TCACAGCTGT GTTACACCAA GGAGCCAGTG GAAGAGACCG AGGAGACCCT GGAAAGTCCT
    CCACTTCCTT CCTCTAACTC AGCCCTTCTA TCTTCATATA AAAAACCACC AAGCCCCAGA
    GACAAGCTCT TCAACAAGAG CATGATACTC AGGGCTAGGT CTTCAGAGTG CCTCAGTCAA
    GCCCCTGAGG GTCAAGAATC TAGGATTGGC TTGGAGGGAC GAGTGAGTCC TGGCCAGAGA
    TCTGGCCAGT CCTCTTTGAG CCCAAACACC TGGTGGAAAG CATCTGACCC ATCCTCGTCA
    GACTCTGAAA AAACAAACGT TCACCATGGA GTCTGTGGAG GTCACTGGAG ATGGTCACCA
    GAGCACAACC TGCAGCCACA TGTGGCACTA GCCATGGAAG GCCCTTCCAA CCCAGGTGAC
    AAGGAATTGA AAGCGTCTAC TGTCCAAGCT GGGGAGGAAG CCATCCTCTT GCCCTTTGCA
    GACAGAAGAA AGTTCTTTGA AGAGAGTAGC AAATCCTTAT CTACATCTCA TTTGCCAGGT
    TTAACCACTC ATAACAAGAC TTTTACCCAG AGACCAAAAC CTATTGACCA AAGCTTTCAG
    CCAATGAGCT CCAGCTATAG AGAATTGAGG CGCCATCCCA TAGACCAATC ATATCACTCC
    ACAGACCAAC CCTATCATGC CACAGACCAA TCATATCATT CCATATCACC CCTTCAGTCA
    GAAACTCCCA CTTACACAGA ATGTTTTGCA AGCAAAGGTC TAGAACAATC CATGTGCTGT
    AAGCCACTAC ACTGTGGTGA TTTTGATTAC CACAGGACGT GCTCTTACTC CTGCAATGTC
    CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG TCCTGCTTTG
    CGAAAGAGAA ATATGATTCC AAACTGCTAC AATTGCCGGT GCCACCATCA CCAGTGCATC
    CGGTGTTCAG CTTGCTGTCA TAATCCCCAG CATGGTACCC TCGAGGACAG CAGCTTGAAA
    CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG
    AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG
    GCTAGCTTTT CATGGGCAAC CCCATTCCAT CCTTGCCTTG AGAACCCAGC ACTGGACTTG
    TCAAACTACC GAGCAGTCTC TTCTCTTGAC CTCCTTGGAG ACTTTAAGCA CTCCTCCAAA
    AAAACAGAGG AAACCTCCGT TTATGAAGAG GAGAGCTCCC TAGCCTCCAT GCCTCGCCCA
    CTGCGCAGCC GTGCCTTCTC AGAAAGCCAC ATCACCCTGG ATCCTCAGAG TTCCCAGGTC
    TGGGAGCAGC ATAGGAAGGA GCTCTTCAAC AAAGTTGATG AGACCCAGCC AGATGCTCTG
    GGTGCCAGGA AGAAGGCCTT TCCTCCTCCT CGTCCTCCTC CTCCCAACTG GGAGAAGTAC
    AGGCTCTTCC GTGCAGCCCA GCAACAGCAA CAGCAGCAAA AGCAGCAGCA GCAAGAGGAG
    GAAGAAGAGG AGGAGGAGGA AGAGGAAGAA GAAGAGGAGG AAGGGGTAGA GGAGGAGGAA
    GAAGAGGAGG AAGCGGGGGA GGAGGAGGAG CTGCCACCCC AGTATTTCAG TTCAGAAACC
    ACTGGTTCTG GTGCCCTCCA TCCTGAGGAG GTTCTGGAGC AGCCCCAGCT TCCGGCCTTT
    GGCCACCTGG AGGCCCCGAG GCAGGGCTCA CAAAGCCTCC CAGCAGAGCC AGAATCCTTT
    GCTCTGCGTT CCAGTGATTT CCTGCCTCCA GTAAGGGGCC ACTTGGGATC TCAAGCTGAA
    AAGGCTCAAC CTCCTTGTTA TTATGGCATT GGTGGGCTTT GGAGGACATC AGAGCAGGAG
    ACCACTGATT CCAAACCAGA AGCTTTGATG GCGGAAGAGT CCAAATCCAA ACCAGCATGG
    AGCCAGAGCT ACTTCTTTCC TGAGGAGCAG TTCTCTTTCA TGGGCTGGGG CTCAGATAAG
    CAGCACCTCA ACCCTTCAGG CTTCAGTGAA CCCAAGACAA CAGGACAAGA TTTTCAGCAC
    TTCTCACCTC CTCAGGGGGC CCCAGGGATC CCTACCTCTT ATTCAGCTTA TTACAATATT
    TCTGTGGCCA AAGCAGAGCT GTTGAACAAG TTGAAAGACC AACCTGAGAT GTTAGAGATT
    GGCCTAGGAG AGGAGGAAAT TGACCACGAA CTGGCTCAAA AAAAGGTACA GCTTATTGAC
    AGCATTGGCA GAAAACTCTC TGTCCTGCGG GAGGCCCAGC GAGGACTGCT GGAAGACATC
    ACTGCCAATT CTGCCCTTGG GGAGGAGGTG GAGGCCAACC TAAAAGCCCT CTGCAAATCC
    AATGAGTTTG AAAAATACCG TTTATTTATC GGGGACCTGG ACAAAGTAGT CAACCTGTTG
    CTGTCACTCT CTGGTCGGCT GGCACGGGTG GAGAATGCTC TCAACAGCAT CGATTCAGAG
    GCCAACCAGG AGAAGTTGGT GCTGATAGAG AAGAAGCAGC AGCTGACAGG GCAGTTGGCA
    GATGCCAAGG AGCTGAAGGA GCATGTGGAC CGTCGGGAGA AGTTGGTGTT TGGCATGGTC
    TCCCGTTACC TGCCTCAGGA CCAGCTCCAA GATTACCAGC ACTTTGTGAA GATGAAATCT
    GCTCTCATCA TTGAACAGCG AGAGCTGGAA GAGAAGATCA AGCTCGGGGA AGAACAGCTC
    AAATGTCTCA GGGAAAGCCT GCTCCTGGGG CCCAGTAATT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325955.1
    CDS241..4725
    Translation

    Target ORF information:

    RefSeq Version XM_015470469.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTACGG CTCCCGCCAA 
    GAGGCTCTCA TCCTCATCAA AGGCTCCTTC CGGACCCTCA AGCTGATTGT CAGGAGGAGA
    AACGCCCCTG TCAGTAGGCC GCACTCATGG CATGTGGCCA AGCTGCTGGA GGGATGCCCT
    GAAGCGGCCA CCACCATGCA TTTCCCTTCT GAAGCCTTCA GCTTGTCCTG GCATTCCGGC
    TGCAACACAA GTGACGTGTG TGTGCAGTGG TGTCCACTCT CCCGGCACTG CAGCACTGAG
    AAAAGCAGCT CCATTGGCAG TATGGAGAGC CTGGAGCAAC CAGGCCAAGC CACCTATGAG
    GGCCATCTTT TGCCCATTGA CCAGAGCACA TACCCCAACC AGCGTGATTC AGCCTATAGC
    TCCTTCTCAG CCAGCTCAAA TGCCTCTGAC TGTGCCCTTT CCCTCAGGCC AGAGGAGCCA
    GCCTCTGTGG ACTGTATCAT GCAAGGCCCA GGGCCAACTA AAGCCCCTAA TAGTCGCCCT
    AATGTGGTCG AGACCTCAGG AGGTGGCTGG CGCACCAGTG GGGGTCACCT GACTGCTAGC
    TCCCAGATGT CATCCTGTCC TCAGGAGGTC TCCCACTCAG AGCCTGCCAA GGCTGCCAGG
    GGTCCCCCTC AACCTCCAGT GAGGCGAGAC AGTCTTCAGG CCTCCAGAGC CCAACTCTTC
    AATGGAGAGC AGTGCAGGGC TTCTGAGCTT ATAGATTCCT TGCAACAGAA GGAGAAACCT
    AGCTTGGACA CCATGCTATC CCCAAGGAAC CCTAATAGGT TCTGCTGCCT CAGCGGGCAA
    GACCAAGTGA CAAATGAAGG CCATCAGAAC TGTGAGCTCA GCCAACCTCC TGAATCTAAC
    CAGAAGGGCT CTGAGAATCT ACCGATGGAG GCCTCAGCCA AAGCTGTTGG AGTCCCAAAA
    GCATGTGACA AAACTTCCAG CATAGGTTCC AGCCCACTCA ACCAGGCTTC AGCAGAGCTA
    GCTAAGACTT CTTCTTTTGG CAGCTCTCCA CACCTCACAG GACCCACAGG GCATCGCCAT
    AGTGCCCCTG AACAGCTGCT GGTGTCCCAC TTGCAGCGTG TGCACCTTGA TACTAGGGAC
    AGCAGGGGGA CAGAGCTCCA AGCTGGGCAG GATGGGCAGG AGTGGACCGT ATCCCCTTTG
    CACATGCACA GTAGCCACAC AAGCAAGAAA AGCCCATGTG CCCCTGCAGG AGGAACCCAG
    GACCAGCCGA GCAAAGACAG AAAGACCAAG CCAGTGGATG ATAGACCTCT GGGTTCAGGT
    CATCAGAGCC CAAGCAGTTC CCCACATGGA GAGGCTGACG GACACCATCC AGAAAAAGGC
    TTCCAGGATC CAAACAGAGC AAGCAGAGCT GATAGTGAAC TGGTCAGCCA GCAGTCCTCT
    GGCTCCCTTG CTCAACAAAC CAGGGACTGT TCTTCAACCT CTAAAGTAGC TGGCAGCACA
    GAGGCAACTG AGGAAGGGGA CAGTGAGCCC AAGGAGTGTG GCCGGGTGGG CAGTAAGCGA
    AATGGAGGGT TCCGGGGCCG CTCGATCCAA AACCGGCGGA AGAGCGAACG TTTTGCTACC
    AATCTACGTA ATGAAATTCA GAGGAGGAAG GCCCAGCTCC AGAAAGGCAA GAGTCCCTTG
    TCACAGCTGT GTTACACCAA GGAGCCAGTG GAAGAGACCG AGGAGACCCT GGAAAGTCCT
    CCACTTCCTT CCTCTAACTC AGCCCTTCTA TCTTCATATA AAAAACCACC AAGCCCCAGA
    GACAAGCTCT TCAACAAGAG CATGATACTC AGGGCTAGGT CTTCAGAGTG CCTCAGTCAA
    GCCCCTGAGG GTCAAGAATC TAGGATTGGC TTGGAGGGAC GAGTGAGTCC TGGCCAGAGA
    TCTGGCCAGT CCTCTTTGAG CCCAAACACC TGGTGGAAAG CATCTGACCC ATCCTCGTCA
    GACTCTGAAA AAACAAACGT TCACCATGGA GTCTGTGGAG GTCACTGGAG ATGGTCACCA
    GAGCACAACC TGCAGCCACA TGTGGCACTA GCCATGGAAG GCCCTTCCAA CCCAGGTGAC
    AAGGAATTGA AAGCGTCTAC TGTCCAAGCT GGGGAGGAAG CCATCCTCTT GCCCTTTGCA
    GACAGAAGAA AGTTCTTTGA AGAGAGTAGC AAATCCTTAT CTACATCTCA TTTGCCAGGT
    TTAACCACTC ATAACAAGAC TTTTACCCAG AGACCAAAAC CTATTGACCA AAGCTTTCAG
    CCAATGAGCT CCAGCTATAG AGAATTGAGG CGCCATCCCA TAGACCAATC ATATCACTCC
    ACAGACCAAC CCTATCATGC CACAGACCAA TCATATCATT CCATATCACC CCTTCAGTCA
    GAAACTCCCA CTTACACAGA ATGTTTTGCA AGCAAAGGTC TAGAACAATC CATGTGCTGT
    AAGCCACTAC ACTGTGGTGA TTTTGATTAC CACAGGACGT GCTCTTACTC CTGCAATGTC
    CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG TCCTGCTTTG
    CGAAAGAGAA ATATGATTCC AAACTGCTAC AATTGCCGGT GCCACCATCA CCAGTGCATC
    CGGTGTTCAG CTTGCTGTCA TAATCCCCAG CATGGTACCC TCGAGGACAG CAGCTTGAAA
    CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG
    AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG
    GCTAGCTTTT CATGGGCAAC CCCATTCCAT CCTTGCCTTG AGAACCCAGC ACTGGACTTG
    TCAAACTACC GAGCAGTCTC TTCTCTTGAC CTCCTTGGAG ACTTTAAGCA CTCCTCCAAA
    AAAACAGAGG AAACCTCCGT TTATGAAGAG GAGAGCTCCC TAGCCTCCAT GCCTCGCCCA
    CTGCGCAGCC GTGCCTTCTC AGAAAGCCAC ATCACCCTGG ATCCTCAGAG TTCCCAGGTC
    TGGGAGCAGC ATAGGAAGGA GCTCTTCAAC AAAGTTGATG AGACCCAGCC AGATGCTCTG
    GGTGCCAGGA AGAAGGCCTT TCCTCCTCCT CGTCCTCCTC CTCCCAACTG GGAGAAGTAC
    AGGCTCTTCC GTGCAGCCCA GCAACAGCAA CAGCAGCAAA AGCAGCAGCA GCAAGAGGAG
    GAAGAAGAGG AGGAGGAGGA AGAGGAAGAA GAAGAGGAGG AAGGGGTAGA GGAGGAGGAA
    GAAGAGGAGG AAGCGGGGGA GGAGGAGGAG CTGCCACCCC AGTATTTCAG TTCAGAAACC
    ACTGGTTCTG GTGCCCTCCA TCCTGAGGAG GTTCTGGAGC AGCCCCAGCT TCCGGCCTTT
    GGCCACCTGG AGGCCCCGAG GCAGGGCTCA CAAAGCCTCC CAGCAGAGCC AGAATCCTTT
    GCTCTGCGTT CCAGTGATTT CCTGCCTCCA GTAAGGGGCC ACTTGGGATC TCAAGCTGAA
    AAGGCTCAAC CTCCTTGTTA TTATGGCATT GGTGGGCTTT GGAGGACATC AGAGCAGGAG
    ACCACTGATT CCAAACCAGA AGCTTTGATG GCGGAAGAGT CCAAATCCAA ACCAGCATGG
    AGCCAGAGCT ACTTCTTTCC TGAGGAGCAG TTCTCTTTCA TGGGCTGGGG CTCAGATAAG
    CAGCACCTCA ACCCTTCAGG CTTCAGTGAA CCCAAGACAA CAGGACAAGA TTTTCAGCAC
    TTCTCACCTC CTCAGGGGGC CCCAGGGATC CCTACCTCTT ATTCAGCTTA TTACAATATT
    TCTGTGGCCA AAGCAGAGCT GTTGAACAAG TTGAAAGACC AACCTGAGAT GTTAGAGATT
    GGCCTAGGAG AGGAGGAAAT TGACCACGAA CTGGCTCAAA AAAAGGTACA GCTTATTGAC
    AGCATTGGCA GAAAACTCTC TGTCCTGCGG GAGGCCCAGC GAGGACTGCT GGAAGACATC
    ACTGCCAATT CTGCCCTTGG GGAGGAGGTG GAGGCCAACC TAAAAGCCCT CTGCAAATCC
    AATGAGTTTG AAAAATACCG TTTATTTATC GGGGACCTGG ACAAAGTAGT CAACCTGTTG
    CTGTCACTCT CTGGTCGGCT GGCACGGGTG GAGAATGCTC TCAACAGCAT CGATTCAGAG
    GCCAACCAGG AGAAGTTGGT GCTGATAGAG AAGAAGCAGC AGCTGACAGG GCAGTTGGCA
    GATGCCAAGG AGCTGAAGGA GCATGTGGAC CGTCGGGAGA AGTTGGTGTT TGGCATGGTC
    TCCCGTTACC TGCCTCAGGA CCAGCTCCAA GATTACCAGC ACTTTGTGAA GATGAAATCT
    GCTCTCATCA TTGAACAGCG AGAGCTGGAA GAGAAGATCA AGCTCGGGGA AGAACAGCTC
    AAATGTCTCA GGGAAAGCCT GCTCCTGGGG CCCAGTAATT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89376
    Clone ID Related Accession (Same CDS sequence) XM_015470469.1 , XM_015461686.2 , XM_015461686.1
    Accession Version XM_015461686.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTACGG CTCCCGCCAA 
    GAGGCTCTCA TCCTCATCAA AGGCTCCTTC CGGACCCTCA AGCTGATTGT CAGGAGGAGA
    AACGCCCCTG TCAGTAGGCC GCACTCATGG CATGTGGCCA AGCTGCTGGA GGGATGCCCT
    GAAGCGGCCA CCACCATGCA TTTCCCTTCT GAAGCCTTCA GCTTGTCCTG GCATTCCGGC
    TGCAACACAA GTGACGTGTG TGTGCAGTGG TGTCCACTCT CCCGGCACTG CAGCACTGAG
    AAAAGCAGCT CCATTGGCAG TATGGAGAGC CTGGAGCAAC CAGGCCAAGC CACCTATGAG
    GGCCATCTTT TGCCCATTGA CCAGAGCACA TACCCCAACC AGCGTGATTC AGCCTATAGC
    TCCTTCTCAG CCAGCTCAAA TGCCTCTGAC TGTGCCCTTT CCCTCAGGCC AGAGGAGCCA
    GCCTCTGTGG ACTGTATCAT GCAAGGCCCA GGGCCAACTA AAGCCCCTAA TAGTCGCCCT
    AATGTGGTCG AGACCTCAGG AGGTGGCTGG CGCACCAGTG GGGGTCACCT GACTGCTAGC
    TCCCAGATGT CATCCTGTCC TCAGGAGGTC TCCCACTCAG AGCCTGCCAA GGCTGCCAGG
    GGTCCCCCTC AACCTCCAGT GAGGCGAGAC AGTCTTCAGG CCTCCAGAGC CCAACTCTTC
    AATGGAGAGC AGTGCAGGGC TTCTGAGCTT ATAGATTCCT TGCAACAGAA GGAGAAACCT
    AGCTTGGACA CCATGCTATC CCCAAGGAAC CCTAATAGGT TCTGCTGCCT CAGCGGGCAA
    GACCAAGTGA CAAATGAAGG CCATCAGAAC TGTGAGCTCA GCCAACCTCC TGAATCTAAC
    CAGAAGGGCT CTGAGAATCT ACCGATGGAG GCCTCAGCCA AAGCTGTTGG AGTCCCAAAA
    GCATGTGACA AAACTTCCAG CATAGGTTCC AGCCCACTCA ACCAGGCTTC AGCAGAGCTA
    GCTAAGACTT CTTCTTTTGG CAGCTCTCCA CACCTCACAG GACCCACAGG GCATCGCCAT
    AGTGCCCCTG AACAGCTGCT GGTGTCCCAC TTGCAGCGTG TGCACCTTGA TACTAGGGAC
    AGCAGGGGGA CAGAGCTCCA AGCTGGGCAG GATGGGCAGG AGTGGACCGT ATCCCCTTTG
    CACATGCACA GTAGCCACAC AAGCAAGAAA AGCCCATGTG CCCCTGCAGG AGGAACCCAG
    GACCAGCCGA GCAAAGACAG AAAGACCAAG CCAGTGGATG ATAGACCTCT GGGTTCAGGT
    CATCAGAGCC CAAGCAGTTC CCCACATGGA GAGGCTGACG GACACCATCC AGAAAAAGGC
    TTCCAGGATC CAAACAGAGC AAGCAGAGCT GATAGTGAAC TGGTCAGCCA GCAGTCCTCT
    GGCTCCCTTG CTCAACAAAC CAGGGACTGT TCTTCAACCT CTAAAGTAGC TGGCAGCACA
    GAGGCAACTG AGGAAGGGGA CAGTGAGCCC AAGGAGTGTG GCCGGGTGGG CAGTAAGCGA
    AATGGAGGGT TCCGGGGCCG CTCGATCCAA AACCGGCGGA AGAGCGAACG TTTTGCTACC
    AATCTACGTA ATGAAATTCA GAGGAGGAAG GCCCAGCTCC AGAAAGGCAA GAGTCCCTTG
    TCACAGCTGT GTTACACCAA GGAGCCAGTG GAAGAGACCG AGGAGACCCT GGAAAGTCCT
    CCACTTCCTT CCTCTAACTC AGCCCTTCTA TCTTCATATA AAAAACCACC AAGCCCCAGA
    GACAAGCTCT TCAACAAGAG CATGATACTC AGGGCTAGGT CTTCAGAGTG CCTCAGTCAA
    GCCCCTGAGG GTCAAGAATC TAGGATTGGC TTGGAGGGAC GAGTGAGTCC TGGCCAGAGA
    TCTGGCCAGT CCTCTTTGAG CCCAAACACC TGGTGGAAAG CATCTGACCC ATCCTCGTCA
    GACTCTGAAA AAACAAACGT TCACCATGGA GTCTGTGGAG GTCACTGGAG ATGGTCACCA
    GAGCACAACC TGCAGCCACA TGTGGCACTA GCCATGGAAG GCCCTTCCAA CCCAGGTGAC
    AAGGAATTGA AAGCGTCTAC TGTCCAAGCT GGGGAGGAAG CCATCCTCTT GCCCTTTGCA
    GACAGAAGAA AGTTCTTTGA AGAGAGTAGC AAATCCTTAT CTACATCTCA TTTGCCAGGT
    TTAACCACTC ATAACAAGAC TTTTACCCAG AGACCAAAAC CTATTGACCA AAGCTTTCAG
    CCAATGAGCT CCAGCTATAG AGAATTGAGG CGCCATCCCA TAGACCAATC ATATCACTCC
    ACAGACCAAC CCTATCATGC CACAGACCAA TCATATCATT CCATATCACC CCTTCAGTCA
    GAAACTCCCA CTTACACAGA ATGTTTTGCA AGCAAAGGTC TAGAACAATC CATGTGCTGT
    AAGCCACTAC ACTGTGGTGA TTTTGATTAC CACAGGACGT GCTCTTACTC CTGCAATGTC
    CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG TCCTGCTTTG
    CGAAAGAGAA ATATGATTCC AAACTGCTAC AATTGCCGGT GCCACCATCA CCAGTGCATC
    CGGTGTTCAG CTTGCTGTCA TAATCCCCAG CATGGTACCC TCGAGGACAG CAGCTTGAAA
    CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG
    AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG
    GCTAGCTTTT CATGGGCAAC CCCATTCCAT CCTTGCCTTG AGAACCCAGC ACTGGACTTG
    TCAAACTACC GAGCAGTCTC TTCTCTTGAC CTCCTTGGAG ACTTTAAGCA CTCCTCCAAA
    AAAACAGAGG AAACCTCCGT TTATGAAGAG GAGAGCTCCC TAGCCTCCAT GCCTCGCCCA
    CTGCGCAGCC GTGCCTTCTC AGAAAGCCAC ATCACCCTGG ATCCTCAGAG TTCCCAGGTC
    TGGGAGCAGC ATAGGAAGGA GCTCTTCAAC AAAGTTGATG AGACCCAGCC AGATGCTCTG
    GGTGCCAGGA AGAAGGCCTT TCCTCCTCCT CGTCCTCCTC CTCCCAACTG GGAGAAGTAC
    AGGCTCTTCC GTGCAGCCCA GCAACAGCAA CAGCAGCAAA AGCAGCAGCA GCAAGAGGAG
    GAAGAAGAGG AGGAGGAGGA AGAGGAAGAA GAAGAGGAGG AAGGGGTAGA GGAGGAGGAA
    GAAGAGGAGG AAGCGGGGGA GGAGGAGGAG CTGCCACCCC AGTATTTCAG TTCAGAAACC
    ACTGGTTCTG GTGCCCTCCA TCCTGAGGAG GTTCTGGAGC AGCCCCAGCT TCCGGCCTTT
    GGCCACCTGG AGGCCCCGAG GCAGGGCTCA CAAAGCCTCC CAGCAGAGCC AGAATCCTTT
    GCTCTGCGTT CCAGTGATTT CCTGCCTCCA GTAAGGGGCC ACTTGGGATC TCAAGCTGAA
    AAGGCTCAAC CTCCTTGTTA TTATGGCATT GGTGGGCTTT GGAGGACATC AGAGCAGGAG
    ACCACTGATT CCAAACCAGA AGCTTTGATG GCGGAAGAGT CCAAATCCAA ACCAGCATGG
    AGCCAGAGCT ACTTCTTTCC TGAGGAGCAG TTCTCTTTCA TGGGCTGGGG CTCAGATAAG
    CAGCACCTCA ACCCTTCAGG CTTCAGTGAA CCCAAGACAA CAGGACAAGA TTTTCAGCAC
    TTCTCACCTC CTCAGGGGGC CCCAGGGATC CCTACCTCTT ATTCAGCTTA TTACAATATT
    TCTGTGGCCA AAGCAGAGCT GTTGAACAAG TTGAAAGACC AACCTGAGAT GTTAGAGATT
    GGCCTAGGAG AGGAGGAAAT TGACCACGAA CTGGCTCAAA AAAAGGTACA GCTTATTGAC
    AGCATTGGCA GAAAACTCTC TGTCCTGCGG GAGGCCCAGC GAGGACTGCT GGAAGACATC
    ACTGCCAATT CTGCCCTTGG GGAGGAGGTG GAGGCCAACC TAAAAGCCCT CTGCAAATCC
    AATGAGTTTG AAAAATACCG TTTATTTATC GGGGACCTGG ACAAAGTAGT CAACCTGTTG
    CTGTCACTCT CTGGTCGGCT GGCACGGGTG GAGAATGCTC TCAACAGCAT CGATTCAGAG
    GCCAACCAGG AGAAGTTGGT GCTGATAGAG AAGAAGCAGC AGCTGACAGG GCAGTTGGCA
    GATGCCAAGG AGCTGAAGGA GCATGTGGAC CGTCGGGAGA AGTTGGTGTT TGGCATGGTC
    TCCCGTTACC TGCCTCAGGA CCAGCTCCAA GATTACCAGC ACTTTGTGAA GATGAAATCT
    GCTCTCATCA TTGAACAGCG AGAGCTGGAA GAGAAGATCA AGCTCGGGGA AGAACAGCTC
    AAATGTCTCA GGGAAAGCCT GCTCCTGGGG CCCAGTAATT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317172.1
    CDS245..4729
    Translation

    Target ORF information:

    RefSeq Version XM_015461686.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461686.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTACGG CTCCCGCCAA 
    GAGGCTCTCA TCCTCATCAA AGGCTCCTTC CGGACCCTCA AGCTGATTGT CAGGAGGAGA
    AACGCCCCTG TCAGTAGGCC GCACTCATGG CATGTGGCCA AGCTGCTGGA GGGATGCCCT
    GAAGCGGCCA CCACCATGCA TTTCCCTTCT GAAGCCTTCA GCTTGTCCTG GCATTCCGGC
    TGCAACACAA GTGACGTGTG TGTGCAGTGG TGTCCACTCT CCCGGCACTG CAGCACTGAG
    AAAAGCAGCT CCATTGGCAG TATGGAGAGC CTGGAGCAAC CAGGCCAAGC CACCTATGAG
    GGCCATCTTT TGCCCATTGA CCAGAGCACA TACCCCAACC AGCGTGATTC AGCCTATAGC
    TCCTTCTCAG CCAGCTCAAA TGCCTCTGAC TGTGCCCTTT CCCTCAGGCC AGAGGAGCCA
    GCCTCTGTGG ACTGTATCAT GCAAGGCCCA GGGCCAACTA AAGCCCCTAA TAGTCGCCCT
    AATGTGGTCG AGACCTCAGG AGGTGGCTGG CGCACCAGTG GGGGTCACCT GACTGCTAGC
    TCCCAGATGT CATCCTGTCC TCAGGAGGTC TCCCACTCAG AGCCTGCCAA GGCTGCCAGG
    GGTCCCCCTC AACCTCCAGT GAGGCGAGAC AGTCTTCAGG CCTCCAGAGC CCAACTCTTC
    AATGGAGAGC AGTGCAGGGC TTCTGAGCTT ATAGATTCCT TGCAACAGAA GGAGAAACCT
    AGCTTGGACA CCATGCTATC CCCAAGGAAC CCTAATAGGT TCTGCTGCCT CAGCGGGCAA
    GACCAAGTGA CAAATGAAGG CCATCAGAAC TGTGAGCTCA GCCAACCTCC TGAATCTAAC
    CAGAAGGGCT CTGAGAATCT ACCGATGGAG GCCTCAGCCA AAGCTGTTGG AGTCCCAAAA
    GCATGTGACA AAACTTCCAG CATAGGTTCC AGCCCACTCA ACCAGGCTTC AGCAGAGCTA
    GCTAAGACTT CTTCTTTTGG CAGCTCTCCA CACCTCACAG GACCCACAGG GCATCGCCAT
    AGTGCCCCTG AACAGCTGCT GGTGTCCCAC TTGCAGCGTG TGCACCTTGA TACTAGGGAC
    AGCAGGGGGA CAGAGCTCCA AGCTGGGCAG GATGGGCAGG AGTGGACCGT ATCCCCTTTG
    CACATGCACA GTAGCCACAC AAGCAAGAAA AGCCCATGTG CCCCTGCAGG AGGAACCCAG
    GACCAGCCGA GCAAAGACAG AAAGACCAAG CCAGTGGATG ATAGACCTCT GGGTTCAGGT
    CATCAGAGCC CAAGCAGTTC CCCACATGGA GAGGCTGACG GACACCATCC AGAAAAAGGC
    TTCCAGGATC CAAACAGAGC AAGCAGAGCT GATAGTGAAC TGGTCAGCCA GCAGTCCTCT
    GGCTCCCTTG CTCAACAAAC CAGGGACTGT TCTTCAACCT CTAAAGTAGC TGGCAGCACA
    GAGGCAACTG AGGAAGGGGA CAGTGAGCCC AAGGAGTGTG GCCGGGTGGG CAGTAAGCGA
    AATGGAGGGT TCCGGGGCCG CTCGATCCAA AACCGGCGGA AGAGCGAACG TTTTGCTACC
    AATCTACGTA ATGAAATTCA GAGGAGGAAG GCCCAGCTCC AGAAAGGCAA GAGTCCCTTG
    TCACAGCTGT GTTACACCAA GGAGCCAGTG GAAGAGACCG AGGAGACCCT GGAAAGTCCT
    CCACTTCCTT CCTCTAACTC AGCCCTTCTA TCTTCATATA AAAAACCACC AAGCCCCAGA
    GACAAGCTCT TCAACAAGAG CATGATACTC AGGGCTAGGT CTTCAGAGTG CCTCAGTCAA
    GCCCCTGAGG GTCAAGAATC TAGGATTGGC TTGGAGGGAC GAGTGAGTCC TGGCCAGAGA
    TCTGGCCAGT CCTCTTTGAG CCCAAACACC TGGTGGAAAG CATCTGACCC ATCCTCGTCA
    GACTCTGAAA AAACAAACGT TCACCATGGA GTCTGTGGAG GTCACTGGAG ATGGTCACCA
    GAGCACAACC TGCAGCCACA TGTGGCACTA GCCATGGAAG GCCCTTCCAA CCCAGGTGAC
    AAGGAATTGA AAGCGTCTAC TGTCCAAGCT GGGGAGGAAG CCATCCTCTT GCCCTTTGCA
    GACAGAAGAA AGTTCTTTGA AGAGAGTAGC AAATCCTTAT CTACATCTCA TTTGCCAGGT
    TTAACCACTC ATAACAAGAC TTTTACCCAG AGACCAAAAC CTATTGACCA AAGCTTTCAG
    CCAATGAGCT CCAGCTATAG AGAATTGAGG CGCCATCCCA TAGACCAATC ATATCACTCC
    ACAGACCAAC CCTATCATGC CACAGACCAA TCATATCATT CCATATCACC CCTTCAGTCA
    GAAACTCCCA CTTACACAGA ATGTTTTGCA AGCAAAGGTC TAGAACAATC CATGTGCTGT
    AAGCCACTAC ACTGTGGTGA TTTTGATTAC CACAGGACGT GCTCTTACTC CTGCAATGTC
    CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG TCCTGCTTTG
    CGAAAGAGAA ATATGATTCC AAACTGCTAC AATTGCCGGT GCCACCATCA CCAGTGCATC
    CGGTGTTCAG CTTGCTGTCA TAATCCCCAG CATGGTACCC TCGAGGACAG CAGCTTGAAA
    CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG
    AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG
    GCTAGCTTTT CATGGGCAAC CCCATTCCAT CCTTGCCTTG AGAACCCAGC ACTGGACTTG
    TCAAACTACC GAGCAGTCTC TTCTCTTGAC CTCCTTGGAG ACTTTAAGCA CTCCTCCAAA
    AAAACAGAGG AAACCTCCGT TTATGAAGAG GAGAGCTCCC TAGCCTCCAT GCCTCGCCCA
    CTGCGCAGCC GTGCCTTCTC AGAAAGCCAC ATCACCCTGG ATCCTCAGAG TTCCCAGGTC
    TGGGAGCAGC ATAGGAAGGA GCTCTTCAAC AAAGTTGATG AGACCCAGCC AGATGCTCTG
    GGTGCCAGGA AGAAGGCCTT TCCTCCTCCT CGTCCTCCTC CTCCCAACTG GGAGAAGTAC
    AGGCTCTTCC GTGCAGCCCA GCAACAGCAA CAGCAGCAAA AGCAGCAGCA GCAAGAGGAG
    GAAGAAGAGG AGGAGGAGGA AGAGGAAGAA GAAGAGGAGG AAGGGGTAGA GGAGGAGGAA
    GAAGAGGAGG AAGCGGGGGA GGAGGAGGAG CTGCCACCCC AGTATTTCAG TTCAGAAACC
    ACTGGTTCTG GTGCCCTCCA TCCTGAGGAG GTTCTGGAGC AGCCCCAGCT TCCGGCCTTT
    GGCCACCTGG AGGCCCCGAG GCAGGGCTCA CAAAGCCTCC CAGCAGAGCC AGAATCCTTT
    GCTCTGCGTT CCAGTGATTT CCTGCCTCCA GTAAGGGGCC ACTTGGGATC TCAAGCTGAA
    AAGGCTCAAC CTCCTTGTTA TTATGGCATT GGTGGGCTTT GGAGGACATC AGAGCAGGAG
    ACCACTGATT CCAAACCAGA AGCTTTGATG GCGGAAGAGT CCAAATCCAA ACCAGCATGG
    AGCCAGAGCT ACTTCTTTCC TGAGGAGCAG TTCTCTTTCA TGGGCTGGGG CTCAGATAAG
    CAGCACCTCA ACCCTTCAGG CTTCAGTGAA CCCAAGACAA CAGGACAAGA TTTTCAGCAC
    TTCTCACCTC CTCAGGGGGC CCCAGGGATC CCTACCTCTT ATTCAGCTTA TTACAATATT
    TCTGTGGCCA AAGCAGAGCT GTTGAACAAG TTGAAAGACC AACCTGAGAT GTTAGAGATT
    GGCCTAGGAG AGGAGGAAAT TGACCACGAA CTGGCTCAAA AAAAGGTACA GCTTATTGAC
    AGCATTGGCA GAAAACTCTC TGTCCTGCGG GAGGCCCAGC GAGGACTGCT GGAAGACATC
    ACTGCCAATT CTGCCCTTGG GGAGGAGGTG GAGGCCAACC TAAAAGCCCT CTGCAAATCC
    AATGAGTTTG AAAAATACCG TTTATTTATC GGGGACCTGG ACAAAGTAGT CAACCTGTTG
    CTGTCACTCT CTGGTCGGCT GGCACGGGTG GAGAATGCTC TCAACAGCAT CGATTCAGAG
    GCCAACCAGG AGAAGTTGGT GCTGATAGAG AAGAAGCAGC AGCTGACAGG GCAGTTGGCA
    GATGCCAAGG AGCTGAAGGA GCATGTGGAC CGTCGGGAGA AGTTGGTGTT TGGCATGGTC
    TCCCGTTACC TGCCTCAGGA CCAGCTCCAA GATTACCAGC ACTTTGTGAA GATGAAATCT
    GCTCTCATCA TTGAACAGCG AGAGCTGGAA GAGAAGATCA AGCTCGGGGA AGAACAGCTC
    AAATGTCTCA GGGAAAGCCT GCTCCTGGGG CCCAGTAATT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89376
    Clone ID Related Accession (Same CDS sequence) XM_015470469.1 , XM_015461686.2 , XM_015461686.1
    Accession Version XM_015461686.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104731.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTACGG CTCCCGCCAA 
    GAGGCTCTCA TCCTCATCAA AGGCTCCTTC CGGACCCTCA AGCTGATTGT CAGGAGGAGA
    AACGCCCCTG TCAGTAGGCC GCACTCATGG CATGTGGCCA AGCTGCTGGA GGGATGCCCT
    GAAGCGGCCA CCACCATGCA TTTCCCTTCT GAAGCCTTCA GCTTGTCCTG GCATTCCGGC
    TGCAACACAA GTGACGTGTG TGTGCAGTGG TGTCCACTCT CCCGGCACTG CAGCACTGAG
    AAAAGCAGCT CCATTGGCAG TATGGAGAGC CTGGAGCAAC CAGGCCAAGC CACCTATGAG
    GGCCATCTTT TGCCCATTGA CCAGAGCACA TACCCCAACC AGCGTGATTC AGCCTATAGC
    TCCTTCTCAG CCAGCTCAAA TGCCTCTGAC TGTGCCCTTT CCCTCAGGCC AGAGGAGCCA
    GCCTCTGTGG ACTGTATCAT GCAAGGCCCA GGGCCAACTA AAGCCCCTAA TAGTCGCCCT
    AATGTGGTCG AGACCTCAGG AGGTGGCTGG CGCACCAGTG GGGGTCACCT GACTGCTAGC
    TCCCAGATGT CATCCTGTCC TCAGGAGGTC TCCCACTCAG AGCCTGCCAA GGCTGCCAGG
    GGTCCCCCTC AACCTCCAGT GAGGCGAGAC AGTCTTCAGG CCTCCAGAGC CCAACTCTTC
    AATGGAGAGC AGTGCAGGGC TTCTGAGCTT ATAGATTCCT TGCAACAGAA GGAGAAACCT
    AGCTTGGACA CCATGCTATC CCCAAGGAAC CCTAATAGGT TCTGCTGCCT CAGCGGGCAA
    GACCAAGTGA CAAATGAAGG CCATCAGAAC TGTGAGCTCA GCCAACCTCC TGAATCTAAC
    CAGAAGGGCT CTGAGAATCT ACCGATGGAG GCCTCAGCCA AAGCTGTTGG AGTCCCAAAA
    GCATGTGACA AAACTTCCAG CATAGGTTCC AGCCCACTCA ACCAGGCTTC AGCAGAGCTA
    GCTAAGACTT CTTCTTTTGG CAGCTCTCCA CACCTCACAG GACCCACAGG GCATCGCCAT
    AGTGCCCCTG AACAGCTGCT GGTGTCCCAC TTGCAGCGTG TGCACCTTGA TACTAGGGAC
    AGCAGGGGGA CAGAGCTCCA AGCTGGGCAG GATGGGCAGG AGTGGACCGT ATCCCCTTTG
    CACATGCACA GTAGCCACAC AAGCAAGAAA AGCCCATGTG CCCCTGCAGG AGGAACCCAG
    GACCAGCCGA GCAAAGACAG AAAGACCAAG CCAGTGGATG ATAGACCTCT GGGTTCAGGT
    CATCAGAGCC CAAGCAGTTC CCCACATGGA GAGGCTGACG GACACCATCC AGAAAAAGGC
    TTCCAGGATC CAAACAGAGC AAGCAGAGCT GATAGTGAAC TGGTCAGCCA GCAGTCCTCT
    GGCTCCCTTG CTCAACAAAC CAGGGACTGT TCTTCAACCT CTAAAGTAGC TGGCAGCACA
    GAGGCAACTG AGGAAGGGGA CAGTGAGCCC AAGGAGTGTG GCCGGGTGGG CAGTAAGCGA
    AATGGAGGGT TCCGGGGCCG CTCGATCCAA AACCGGCGGA AGAGCGAACG TTTTGCTACC
    AATCTACGTA ATGAAATTCA GAGGAGGAAG GCCCAGCTCC AGAAAGGCAA GAGTCCCTTG
    TCACAGCTGT GTTACACCAA GGAGCCAGTG GAAGAGACCG AGGAGACCCT GGAAAGTCCT
    CCACTTCCTT CCTCTAACTC AGCCCTTCTA TCTTCATATA AAAAACCACC AAGCCCCAGA
    GACAAGCTCT TCAACAAGAG CATGATACTC AGGGCTAGGT CTTCAGAGTG CCTCAGTCAA
    GCCCCTGAGG GTCAAGAATC TAGGATTGGC TTGGAGGGAC GAGTGAGTCC TGGCCAGAGA
    TCTGGCCAGT CCTCTTTGAG CCCAAACACC TGGTGGAAAG CATCTGACCC ATCCTCGTCA
    GACTCTGAAA AAACAAACGT TCACCATGGA GTCTGTGGAG GTCACTGGAG ATGGTCACCA
    GAGCACAACC TGCAGCCACA TGTGGCACTA GCCATGGAAG GCCCTTCCAA CCCAGGTGAC
    AAGGAATTGA AAGCGTCTAC TGTCCAAGCT GGGGAGGAAG CCATCCTCTT GCCCTTTGCA
    GACAGAAGAA AGTTCTTTGA AGAGAGTAGC AAATCCTTAT CTACATCTCA TTTGCCAGGT
    TTAACCACTC ATAACAAGAC TTTTACCCAG AGACCAAAAC CTATTGACCA AAGCTTTCAG
    CCAATGAGCT CCAGCTATAG AGAATTGAGG CGCCATCCCA TAGACCAATC ATATCACTCC
    ACAGACCAAC CCTATCATGC CACAGACCAA TCATATCATT CCATATCACC CCTTCAGTCA
    GAAACTCCCA CTTACACAGA ATGTTTTGCA AGCAAAGGTC TAGAACAATC CATGTGCTGT
    AAGCCACTAC ACTGTGGTGA TTTTGATTAC CACAGGACGT GCTCTTACTC CTGCAATGTC
    CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG TCCTGCTTTG
    CGAAAGAGAA ATATGATTCC AAACTGCTAC AATTGCCGGT GCCACCATCA CCAGTGCATC
    CGGTGTTCAG CTTGCTGTCA TAATCCCCAG CATGGTACCC TCGAGGACAG CAGCTTGAAA
    CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG
    AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG
    GCTAGCTTTT CATGGGCAAC CCCATTCCAT CCTTGCCTTG AGAACCCAGC ACTGGACTTG
    TCAAACTACC GAGCAGTCTC TTCTCTTGAC CTCCTTGGAG ACTTTAAGCA CTCCTCCAAA
    AAAACAGAGG AAACCTCCGT TTATGAAGAG GAGAGCTCCC TAGCCTCCAT GCCTCGCCCA
    CTGCGCAGCC GTGCCTTCTC AGAAAGCCAC ATCACCCTGG ATCCTCAGAG TTCCCAGGTC
    TGGGAGCAGC ATAGGAAGGA GCTCTTCAAC AAAGTTGATG AGACCCAGCC AGATGCTCTG
    GGTGCCAGGA AGAAGGCCTT TCCTCCTCCT CGTCCTCCTC CTCCCAACTG GGAGAAGTAC
    AGGCTCTTCC GTGCAGCCCA GCAACAGCAA CAGCAGCAAA AGCAGCAGCA GCAAGAGGAG
    GAAGAAGAGG AGGAGGAGGA AGAGGAAGAA GAAGAGGAGG AAGGGGTAGA GGAGGAGGAA
    GAAGAGGAGG AAGCGGGGGA GGAGGAGGAG CTGCCACCCC AGTATTTCAG TTCAGAAACC
    ACTGGTTCTG GTGCCCTCCA TCCTGAGGAG GTTCTGGAGC AGCCCCAGCT TCCGGCCTTT
    GGCCACCTGG AGGCCCCGAG GCAGGGCTCA CAAAGCCTCC CAGCAGAGCC AGAATCCTTT
    GCTCTGCGTT CCAGTGATTT CCTGCCTCCA GTAAGGGGCC ACTTGGGATC TCAAGCTGAA
    AAGGCTCAAC CTCCTTGTTA TTATGGCATT GGTGGGCTTT GGAGGACATC AGAGCAGGAG
    ACCACTGATT CCAAACCAGA AGCTTTGATG GCGGAAGAGT CCAAATCCAA ACCAGCATGG
    AGCCAGAGCT ACTTCTTTCC TGAGGAGCAG TTCTCTTTCA TGGGCTGGGG CTCAGATAAG
    CAGCACCTCA ACCCTTCAGG CTTCAGTGAA CCCAAGACAA CAGGACAAGA TTTTCAGCAC
    TTCTCACCTC CTCAGGGGGC CCCAGGGATC CCTACCTCTT ATTCAGCTTA TTACAATATT
    TCTGTGGCCA AAGCAGAGCT GTTGAACAAG TTGAAAGACC AACCTGAGAT GTTAGAGATT
    GGCCTAGGAG AGGAGGAAAT TGACCACGAA CTGGCTCAAA AAAAGGTACA GCTTATTGAC
    AGCATTGGCA GAAAACTCTC TGTCCTGCGG GAGGCCCAGC GAGGACTGCT GGAAGACATC
    ACTGCCAATT CTGCCCTTGG GGAGGAGGTG GAGGCCAACC TAAAAGCCCT CTGCAAATCC
    AATGAGTTTG AAAAATACCG TTTATTTATC GGGGACCTGG ACAAAGTAGT CAACCTGTTG
    CTGTCACTCT CTGGTCGGCT GGCACGGGTG GAGAATGCTC TCAACAGCAT CGATTCAGAG
    GCCAACCAGG AGAAGTTGGT GCTGATAGAG AAGAAGCAGC AGCTGACAGG GCAGTTGGCA
    GATGCCAAGG AGCTGAAGGA GCATGTGGAC CGTCGGGAGA AGTTGGTGTT TGGCATGGTC
    TCCCGTTACC TGCCTCAGGA CCAGCTCCAA GATTACCAGC ACTTTGTGAA GATGAAATCT
    GCTCTCATCA TTGAACAGCG AGAGCTGGAA GAGAAGATCA AGCTCGGGGA AGAACAGCTC
    AAATGTCTCA GGGAAAGCCT GCTCCTGGGG CCCAGTAATT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317172.1
    CDS241..4725
    Translation

    Target ORF information:

    RefSeq Version XM_015461686.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 4 (SHROOM4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461686.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTACGG CTCCCGCCAA 
    GAGGCTCTCA TCCTCATCAA AGGCTCCTTC CGGACCCTCA AGCTGATTGT CAGGAGGAGA
    AACGCCCCTG TCAGTAGGCC GCACTCATGG CATGTGGCCA AGCTGCTGGA GGGATGCCCT
    GAAGCGGCCA CCACCATGCA TTTCCCTTCT GAAGCCTTCA GCTTGTCCTG GCATTCCGGC
    TGCAACACAA GTGACGTGTG TGTGCAGTGG TGTCCACTCT CCCGGCACTG CAGCACTGAG
    AAAAGCAGCT CCATTGGCAG TATGGAGAGC CTGGAGCAAC CAGGCCAAGC CACCTATGAG
    GGCCATCTTT TGCCCATTGA CCAGAGCACA TACCCCAACC AGCGTGATTC AGCCTATAGC
    TCCTTCTCAG CCAGCTCAAA TGCCTCTGAC TGTGCCCTTT CCCTCAGGCC AGAGGAGCCA
    GCCTCTGTGG ACTGTATCAT GCAAGGCCCA GGGCCAACTA AAGCCCCTAA TAGTCGCCCT
    AATGTGGTCG AGACCTCAGG AGGTGGCTGG CGCACCAGTG GGGGTCACCT GACTGCTAGC
    TCCCAGATGT CATCCTGTCC TCAGGAGGTC TCCCACTCAG AGCCTGCCAA GGCTGCCAGG
    GGTCCCCCTC AACCTCCAGT GAGGCGAGAC AGTCTTCAGG CCTCCAGAGC CCAACTCTTC
    AATGGAGAGC AGTGCAGGGC TTCTGAGCTT ATAGATTCCT TGCAACAGAA GGAGAAACCT
    AGCTTGGACA CCATGCTATC CCCAAGGAAC CCTAATAGGT TCTGCTGCCT CAGCGGGCAA
    GACCAAGTGA CAAATGAAGG CCATCAGAAC TGTGAGCTCA GCCAACCTCC TGAATCTAAC
    CAGAAGGGCT CTGAGAATCT ACCGATGGAG GCCTCAGCCA AAGCTGTTGG AGTCCCAAAA
    GCATGTGACA AAACTTCCAG CATAGGTTCC AGCCCACTCA ACCAGGCTTC AGCAGAGCTA
    GCTAAGACTT CTTCTTTTGG CAGCTCTCCA CACCTCACAG GACCCACAGG GCATCGCCAT
    AGTGCCCCTG AACAGCTGCT GGTGTCCCAC TTGCAGCGTG TGCACCTTGA TACTAGGGAC
    AGCAGGGGGA CAGAGCTCCA AGCTGGGCAG GATGGGCAGG AGTGGACCGT ATCCCCTTTG
    CACATGCACA GTAGCCACAC AAGCAAGAAA AGCCCATGTG CCCCTGCAGG AGGAACCCAG
    GACCAGCCGA GCAAAGACAG AAAGACCAAG CCAGTGGATG ATAGACCTCT GGGTTCAGGT
    CATCAGAGCC CAAGCAGTTC CCCACATGGA GAGGCTGACG GACACCATCC AGAAAAAGGC
    TTCCAGGATC CAAACAGAGC AAGCAGAGCT GATAGTGAAC TGGTCAGCCA GCAGTCCTCT
    GGCTCCCTTG CTCAACAAAC CAGGGACTGT TCTTCAACCT CTAAAGTAGC TGGCAGCACA
    GAGGCAACTG AGGAAGGGGA CAGTGAGCCC AAGGAGTGTG GCCGGGTGGG CAGTAAGCGA
    AATGGAGGGT TCCGGGGCCG CTCGATCCAA AACCGGCGGA AGAGCGAACG TTTTGCTACC
    AATCTACGTA ATGAAATTCA GAGGAGGAAG GCCCAGCTCC AGAAAGGCAA GAGTCCCTTG
    TCACAGCTGT GTTACACCAA GGAGCCAGTG GAAGAGACCG AGGAGACCCT GGAAAGTCCT
    CCACTTCCTT CCTCTAACTC AGCCCTTCTA TCTTCATATA AAAAACCACC AAGCCCCAGA
    GACAAGCTCT TCAACAAGAG CATGATACTC AGGGCTAGGT CTTCAGAGTG CCTCAGTCAA
    GCCCCTGAGG GTCAAGAATC TAGGATTGGC TTGGAGGGAC GAGTGAGTCC TGGCCAGAGA
    TCTGGCCAGT CCTCTTTGAG CCCAAACACC TGGTGGAAAG CATCTGACCC ATCCTCGTCA
    GACTCTGAAA AAACAAACGT TCACCATGGA GTCTGTGGAG GTCACTGGAG ATGGTCACCA
    GAGCACAACC TGCAGCCACA TGTGGCACTA GCCATGGAAG GCCCTTCCAA CCCAGGTGAC
    AAGGAATTGA AAGCGTCTAC TGTCCAAGCT GGGGAGGAAG CCATCCTCTT GCCCTTTGCA
    GACAGAAGAA AGTTCTTTGA AGAGAGTAGC AAATCCTTAT CTACATCTCA TTTGCCAGGT
    TTAACCACTC ATAACAAGAC TTTTACCCAG AGACCAAAAC CTATTGACCA AAGCTTTCAG
    CCAATGAGCT CCAGCTATAG AGAATTGAGG CGCCATCCCA TAGACCAATC ATATCACTCC
    ACAGACCAAC CCTATCATGC CACAGACCAA TCATATCATT CCATATCACC CCTTCAGTCA
    GAAACTCCCA CTTACACAGA ATGTTTTGCA AGCAAAGGTC TAGAACAATC CATGTGCTGT
    AAGCCACTAC ACTGTGGTGA TTTTGATTAC CACAGGACGT GCTCTTACTC CTGCAATGTC
    CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG TCCTGCTTTG
    CGAAAGAGAA ATATGATTCC AAACTGCTAC AATTGCCGGT GCCACCATCA CCAGTGCATC
    CGGTGTTCAG CTTGCTGTCA TAATCCCCAG CATGGTACCC TCGAGGACAG CAGCTTGAAA
    CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG
    AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG
    GCTAGCTTTT CATGGGCAAC CCCATTCCAT CCTTGCCTTG AGAACCCAGC ACTGGACTTG
    TCAAACTACC GAGCAGTCTC TTCTCTTGAC CTCCTTGGAG ACTTTAAGCA CTCCTCCAAA
    AAAACAGAGG AAACCTCCGT TTATGAAGAG GAGAGCTCCC TAGCCTCCAT GCCTCGCCCA
    CTGCGCAGCC GTGCCTTCTC AGAAAGCCAC ATCACCCTGG ATCCTCAGAG TTCCCAGGTC
    TGGGAGCAGC ATAGGAAGGA GCTCTTCAAC AAAGTTGATG AGACCCAGCC AGATGCTCTG
    GGTGCCAGGA AGAAGGCCTT TCCTCCTCCT CGTCCTCCTC CTCCCAACTG GGAGAAGTAC
    AGGCTCTTCC GTGCAGCCCA GCAACAGCAA CAGCAGCAAA AGCAGCAGCA GCAAGAGGAG
    GAAGAAGAGG AGGAGGAGGA AGAGGAAGAA GAAGAGGAGG AAGGGGTAGA GGAGGAGGAA
    GAAGAGGAGG AAGCGGGGGA GGAGGAGGAG CTGCCACCCC AGTATTTCAG TTCAGAAACC
    ACTGGTTCTG GTGCCCTCCA TCCTGAGGAG GTTCTGGAGC AGCCCCAGCT TCCGGCCTTT
    GGCCACCTGG AGGCCCCGAG GCAGGGCTCA CAAAGCCTCC CAGCAGAGCC AGAATCCTTT
    GCTCTGCGTT CCAGTGATTT CCTGCCTCCA GTAAGGGGCC ACTTGGGATC TCAAGCTGAA
    AAGGCTCAAC CTCCTTGTTA TTATGGCATT GGTGGGCTTT GGAGGACATC AGAGCAGGAG
    ACCACTGATT CCAAACCAGA AGCTTTGATG GCGGAAGAGT CCAAATCCAA ACCAGCATGG
    AGCCAGAGCT ACTTCTTTCC TGAGGAGCAG TTCTCTTTCA TGGGCTGGGG CTCAGATAAG
    CAGCACCTCA ACCCTTCAGG CTTCAGTGAA CCCAAGACAA CAGGACAAGA TTTTCAGCAC
    TTCTCACCTC CTCAGGGGGC CCCAGGGATC CCTACCTCTT ATTCAGCTTA TTACAATATT
    TCTGTGGCCA AAGCAGAGCT GTTGAACAAG TTGAAAGACC AACCTGAGAT GTTAGAGATT
    GGCCTAGGAG AGGAGGAAAT TGACCACGAA CTGGCTCAAA AAAAGGTACA GCTTATTGAC
    AGCATTGGCA GAAAACTCTC TGTCCTGCGG GAGGCCCAGC GAGGACTGCT GGAAGACATC
    ACTGCCAATT CTGCCCTTGG GGAGGAGGTG GAGGCCAACC TAAAAGCCCT CTGCAAATCC
    AATGAGTTTG AAAAATACCG TTTATTTATC GGGGACCTGG ACAAAGTAGT CAACCTGTTG
    CTGTCACTCT CTGGTCGGCT GGCACGGGTG GAGAATGCTC TCAACAGCAT CGATTCAGAG
    GCCAACCAGG AGAAGTTGGT GCTGATAGAG AAGAAGCAGC AGCTGACAGG GCAGTTGGCA
    GATGCCAAGG AGCTGAAGGA GCATGTGGAC CGTCGGGAGA AGTTGGTGTT TGGCATGGTC
    TCCCGTTACC TGCCTCAGGA CCAGCTCCAA GATTACCAGC ACTTTGTGAA GATGAAATCT
    GCTCTCATCA TTGAACAGCG AGAGCTGGAA GAGAAGATCA AGCTCGGGGA AGAACAGCTC
    AAATGTCTCA GGGAAAGCCT GCTCCTGGGG CCCAGTAATT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.