DGKQ cDNA ORF clone, Bos taurus(cattle)

The following DGKQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239002 NM_001206305.2
Latest version!
Bos taurus diacylglycerol kinase theta (DGKQ), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa235309 XM_024993212.1
Latest version!
Bos taurus diacylglycerol kinase theta (DGKQ), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa235310 XM_024993213.1
Latest version!
Bos taurus diacylglycerol kinase theta (DGKQ), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa01927 NM_001206305.1
Latest version!
Bos taurus diacylglycerol kinase, theta 110kDa (DGKQ), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239002
    Clone ID Related Accession (Same CDS sequence) NM_001206305.2
    Accession Version NM_001206305.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase theta
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000006.1. On May 29, 2018 this sequence version replaced NM_001206305.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GTGAGTGGGG CGGCGGGGAG
    GGCGCCGACG GCAGCGTCCC CGCTGGCCCG GGCCGAGAGG TGGGGACGCC TGAGTCCGGC
    AAGCAGACTC TGAAGATCTT TGACGGCAGC GATGCGATGC AGCGAAACCA CTTCCGTGCA
    GTCACGGTCC CGCGCCTGGC CCGGAGCCAG GAGGTGCTGG AGGCGGCTCT GCGGGCTTAC
    TACATCTCCG AGGACCCTCA GGGCTTCCAG CTGCAGGCAC TCCCCACGCC TGTGCAGCTT
    GGCGACACCG CGGCCGCGGG AAGGGTCTGG AGCACTGGGC CCGCTGAGGA GGAGGGCGGC
    AGAGCGGCTC CCGAGGCCTG GGTCCTCCGT GCTCTGCCCC GCACCCAGGA GATCCTGAAG
    ATCTACCCGG CCTGGCTCAA GGTGGGTGTG GCCTACGTGT CCATCCGTGT GACCCCGCAG
    AGCACTGCCC GCAGCGTGGT GCTGGAGGTC CTCCCGCTGC TCGGACGGCA GGCCGAGGGG
    GCCGAGGGCT TCCAGCTGGT GGAGGTGCTC ATGGGCAGCA GACAAGTCCA GCGCACGGTG
    CTGGCAGACG AGGAGCCCTT GCTTGACCGG CTTCGTGAGA TCCGGCAGAC GTCCCTGCGG
    CAGATGAGCC AGACGCGGTT CTACGTGGCT GAGAGCAGAG TGCCGGCCCC GCGCGTCTCT
    CTGTTTGTGG GCGGTCTGCC GCCAGGCCTG TCCCCCCAGG AGTACCGCAG GTTGCTGGAT
    GAGGCCGTTA CCAGCAAAGC TGCCCTGGTG ACCGTGAGCC ACGTGTATTC CGCCCAAGGT
    GCCGTGGTGC TGGACGTGGC ATGCTTTGCA GAGGCCGAGC GGCTGTACAT GCTGATCAGG
    GACACAGCTG TGCGTGGCCG GCCACTGACT GCCCTGGTGC TCCCGGAGGT GCTGCACACG
    AAGCTGGCCC CAGACTGCCG CCCCCTGCTC GTGTTTGTGA ACCCCAGGAG TGGAGGACTC
    AAGGGCCGCG ACCTGCTCTG CAGTTTCCGG AAGCTGCTTA ACCCCCACCA GGTCTTTGAG
    CTGACCAACG GGGGGCCGCT GCCTGGGTTC CACGTGTTCT CCCGAGTGCC CTGCTTCCGG
    GTGCTGGTGT GCGGCGGGGA CGGCACTGTG GGCTGGGTAC TCGCCGCCCT GGAGGACCTG
    CGGCACCACC TGGCCTGCCC GGAGCCTGCT GTAGCCATCC TGCCCCTGGG CACAGGGAAC
    GACCTGGGCC GGGTCCTGCG CTGGGGCGCG GGCTACAGCG GGGAGGACCC GTTCTCCGTG
    CTGCTGTCAG TGGACGAGGC GGATGCCGTG CTCGTGGACC GCTGGACCAT CCTGCTGGAC
    GCTCACGAGT CTGCCTGTGG GGAGGACAGC GAGGCAGACG CAGAGCCCCC CAAGATCGTG
    CAGATGAGTA ACTACTGTGG GATCGGCATC GACGCAGAGC TGAGCCTGGA CTTCCACCAG
    GCGCGGGAGG AGGAGCCCGG CAAGTTCACA AGCAGGTTCC ACAACAAGGG CGTGTATGTG
    CGGGTCGGGC TGCAGAAGAT CAGCCACTCC CGCAGCCTGC ACAGGGCCCT GCGGCTCCAG
    GTGGAGCAGC AGGAGGTGGA GCTGCCCAGC ATCGAGGGGC TCATCTTCAT CAACATCCCC
    AGCTGGGGCT CAGGGGCCGA CCTGTGGGGT TCCGACAGCG ACTCGAGGTT CGAGAAGCCG
    CGCATGGACG ACGGGCTGCT GGAGGTGGTG GGGGTGACGG GCGTTGTGCA CATGGGCCAG
    GTCCAGAGCG GCCTGCGCTC GGGCATCCGC ATTGCCCAGG GGTCCTACTT CCGCGTCACT
    CTCCTCAAGG CCACGCCCGT GCAGGTGGAT GGTGAACCCT GGGTCCAGGC TCCCGGGCAC
    CTGATCATCT CAGCTGCGGG CCCTAAGGTC CACATGCTCA GGAAGGCGAA GCAGAAGCCC
    AGGAAGGCGG GGCCCGCCAA GGATGCACGA GCAGATGGGG CGCCGGCCCC TGAGGGGGAC
    CCCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193234.2
    CDS1..2829
    Translation

    Target ORF information:

    RefSeq Version NM_001206305.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase theta (DGKQ), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206305.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GTGAGTGGGG CGGCGGGGAG
    GGCGCCGACG GCAGCGTCCC CGCTGGCCCG GGCCGAGAGG TGGGGACGCC TGAGTCCGGC
    AAGCAGACTC TGAAGATCTT TGACGGCAGC GATGCGATGC AGCGAAACCA CTTCCGTGCA
    GTCACGGTCC CGCGCCTGGC CCGGAGCCAG GAGGTGCTGG AGGCGGCTCT GCGGGCTTAC
    TACATCTCCG AGGACCCTCA GGGCTTCCAG CTGCAGGCAC TCCCCACGCC TGTGCAGCTT
    GGCGACACCG CGGCCGCGGG AAGGGTCTGG AGCACTGGGC CCGCTGAGGA GGAGGGCGGC
    AGAGCGGCTC CCGAGGCCTG GGTCCTCCGT GCTCTGCCCC GCACCCAGGA GATCCTGAAG
    ATCTACCCGG CCTGGCTCAA GGTGGGTGTG GCCTACGTGT CCATCCGTGT GACCCCGCAG
    AGCACTGCCC GCAGCGTGGT GCTGGAGGTC CTCCCGCTGC TCGGACGGCA GGCCGAGGGG
    GCCGAGGGCT TCCAGCTGGT GGAGGTGCTC ATGGGCAGCA GACAAGTCCA GCGCACGGTG
    CTGGCAGACG AGGAGCCCTT GCTTGACCGG CTTCGTGAGA TCCGGCAGAC GTCCCTGCGG
    CAGATGAGCC AGACGCGGTT CTACGTGGCT GAGAGCAGAG TGCCGGCCCC GCGCGTCTCT
    CTGTTTGTGG GCGGTCTGCC GCCAGGCCTG TCCCCCCAGG AGTACCGCAG GTTGCTGGAT
    GAGGCCGTTA CCAGCAAAGC TGCCCTGGTG ACCGTGAGCC ACGTGTATTC CGCCCAAGGT
    GCCGTGGTGC TGGACGTGGC ATGCTTTGCA GAGGCCGAGC GGCTGTACAT GCTGATCAGG
    GACACAGCTG TGCGTGGCCG GCCACTGACT GCCCTGGTGC TCCCGGAGGT GCTGCACACG
    AAGCTGGCCC CAGACTGCCG CCCCCTGCTC GTGTTTGTGA ACCCCAGGAG TGGAGGACTC
    AAGGGCCGCG ACCTGCTCTG CAGTTTCCGG AAGCTGCTTA ACCCCCACCA GGTCTTTGAG
    CTGACCAACG GGGGGCCGCT GCCTGGGTTC CACGTGTTCT CCCGAGTGCC CTGCTTCCGG
    GTGCTGGTGT GCGGCGGGGA CGGCACTGTG GGCTGGGTAC TCGCCGCCCT GGAGGACCTG
    CGGCACCACC TGGCCTGCCC GGAGCCTGCT GTAGCCATCC TGCCCCTGGG CACAGGGAAC
    GACCTGGGCC GGGTCCTGCG CTGGGGCGCG GGCTACAGCG GGGAGGACCC GTTCTCCGTG
    CTGCTGTCAG TGGACGAGGC GGATGCCGTG CTCGTGGACC GCTGGACCAT CCTGCTGGAC
    GCTCACGAGT CTGCCTGTGG GGAGGACAGC GAGGCAGACG CAGAGCCCCC CAAGATCGTG
    CAGATGAGTA ACTACTGTGG GATCGGCATC GACGCAGAGC TGAGCCTGGA CTTCCACCAG
    GCGCGGGAGG AGGAGCCCGG CAAGTTCACA AGCAGGTTCC ACAACAAGGG CGTGTATGTG
    CGGGTCGGGC TGCAGAAGAT CAGCCACTCC CGCAGCCTGC ACAGGGCCCT GCGGCTCCAG
    GTGGAGCAGC AGGAGGTGGA GCTGCCCAGC ATCGAGGGGC TCATCTTCAT CAACATCCCC
    AGCTGGGGCT CAGGGGCCGA CCTGTGGGGT TCCGACAGCG ACTCGAGGTT CGAGAAGCCG
    CGCATGGACG ACGGGCTGCT GGAGGTGGTG GGGGTGACGG GCGTTGTGCA CATGGGCCAG
    GTCCAGAGCG GCCTGCGCTC GGGCATCCGC ATTGCCCAGG GGTCCTACTT CCGCGTCACT
    CTCCTCAAGG CCACGCCCGT GCAGGTGGAT GGTGAACCCT GGGTCCAGGC TCCCGGGCAC
    CTGATCATCT CAGCTGCGGG CCCTAAGGTC CACATGCTCA GGAAGGCGAA GCAGAAGCCC
    AGGAAGGCGG GGCCCGCCAA GGATGCACGA GCAGATGGGG CGCCGGCCCC TGAGGGGGAC
    CCCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235309
    Clone ID Related Accession (Same CDS sequence) XM_024993212.1
    Accession Version XM_024993212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase theta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG AGGACGGGGA GGACAGCGCC
    GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG
    ACTCTGAAGA TCTTTGACGG CAGCGATGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG
    GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC
    TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC
    ACCGCGGCCG CGGGAAGGGT CTGGAGCACT GGGCCCGCTG AGGAGGAGGG CGGCAGAGCG
    GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC
    CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT
    GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG
    GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCA
    GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG
    AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT
    GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC
    GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG
    GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA
    GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG
    GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC
    CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTTAACCCCC ACCAGGTCTT TGAGCTGACC
    AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG
    GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCCG CCCTGGAGGA CCTGCGGCAC
    CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG
    GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTCTC CGTGCTGCTG
    TCAGTGGACG AGGCGGATGC CGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC
    GAGTCTGCCT GTGGGGAGGA CAGCGAGGCA GACGCAGAGC CCCCCAAGAT CGTGCAGATG
    AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG
    GAGGAGGAGC CCGGCAAGTT CACAAGCAGG TTCCACAACA AGGGCGTGTA TGTGCGGGTC
    GGGCTGCAGA AGATCAGCCA CTCCCGCAGC CTGCACAGGG CCCTGCGGCT CCAGGTGGAG
    CAGCAGGAGG TGGAGCTGCC CAGCATCGAG GGGCTCATCT TCATCAACAT CCCCAGCTGG
    GGCTCAGGGG CCGACCTGTG GGGTTCCGAC AGCGACTCGA GGTTCGAGAA GCCGCGCATG
    GACGACGGGC TGCTGGAGGT GGTGGGGGTG ACGGGCGTTG TGCACATGGG CCAGGTCCAG
    AGCGGCCTGC GCTCGGGCAT CCGCATTGCC CAGGGGTCCT ACTTCCGCGT CACTCTCCTC
    AAGGCCACGC CCGTGCAGGT GGATGGTGAA CCCTGGGTCC AGGCTCCCGG GCACCTGATC
    ATCTCAGCTG CGGGCCCTAA GGTCCACATG CTCAGGAAGG CGAAGCAGAA GCCCAGGAAG
    GCGGGGCCCG CCAAGGATGC ACGAGCAGAT GGGGCGCCGG CCCCTGAGGG GGACCCCAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848980.1
    CDS111..2933
    Translation

    Target ORF information:

    RefSeq Version XM_024993212.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase theta (DGKQ), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG AGGACGGGGA GGACAGCGCC
    GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG
    ACTCTGAAGA TCTTTGACGG CAGCGATGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG
    GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC
    TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC
    ACCGCGGCCG CGGGAAGGGT CTGGAGCACT GGGCCCGCTG AGGAGGAGGG CGGCAGAGCG
    GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC
    CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT
    GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG
    GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCA
    GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG
    AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT
    GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC
    GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG
    GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA
    GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG
    GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC
    CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTTAACCCCC ACCAGGTCTT TGAGCTGACC
    AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG
    GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCCG CCCTGGAGGA CCTGCGGCAC
    CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG
    GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTCTC CGTGCTGCTG
    TCAGTGGACG AGGCGGATGC CGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC
    GAGTCTGCCT GTGGGGAGGA CAGCGAGGCA GACGCAGAGC CCCCCAAGAT CGTGCAGATG
    AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG
    GAGGAGGAGC CCGGCAAGTT CACAAGCAGG TTCCACAACA AGGGCGTGTA TGTGCGGGTC
    GGGCTGCAGA AGATCAGCCA CTCCCGCAGC CTGCACAGGG CCCTGCGGCT CCAGGTGGAG
    CAGCAGGAGG TGGAGCTGCC CAGCATCGAG GGGCTCATCT TCATCAACAT CCCCAGCTGG
    GGCTCAGGGG CCGACCTGTG GGGTTCCGAC AGCGACTCGA GGTTCGAGAA GCCGCGCATG
    GACGACGGGC TGCTGGAGGT GGTGGGGGTG ACGGGCGTTG TGCACATGGG CCAGGTCCAG
    AGCGGCCTGC GCTCGGGCAT CCGCATTGCC CAGGGGTCCT ACTTCCGCGT CACTCTCCTC
    AAGGCCACGC CCGTGCAGGT GGATGGTGAA CCCTGGGTCC AGGCTCCCGG GCACCTGATC
    ATCTCAGCTG CGGGCCCTAA GGTCCACATG CTCAGGAAGG CGAAGCAGAA GCCCAGGAAG
    GCGGGGCCCG CCAAGGATGC ACGAGCAGAT GGGGCGCCGG CCCCTGAGGG GGACCCCAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235310
    Clone ID Related Accession (Same CDS sequence) XM_024993213.1
    Accession Version XM_024993213.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase theta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG AGGACGGGGA GGACAGCGCC
    GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG
    ACTCTGAAGA TCTTTGACGG CAGCGATGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG
    GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC
    TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC
    ACCGCGGCCG CGGGAAGGGT CTGGAGCACT GGGCCCGCTG AGGAGGAGGG CGGCAGAGCG
    GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC
    CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT
    GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG
    GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCA
    GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG
    AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT
    GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC
    GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG
    GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA
    GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG
    GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC
    CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTTAACCCCC ACCAGGTCTT TGAGCTGACC
    AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG
    GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCCG CCCTGGAGGA CCTGCGGCAC
    CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG
    GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTCTC CGTGCTGCTG
    TCAGTGGACG AGGCGGATGC CGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC
    GAGTCTGCCT GTGGGGAGGA CAGCGAGGCA GACGCAGAGC CCCCCAAGAT CGTGCAGATG
    AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG
    GAGGAGGAGC CCGGCAAGTT CACAAGCAGC TGGGGCTCAG GGGCCGACCT GTGGGGTTCC
    GACAGCGACT CGAGGTTCGA GAAGCCGCGC ATGGACGACG GGCTGCTGGA GGTGGTGGGG
    GTGACGGGCG TTGTGCACAT GGGCCAGGTC CAGAGCGGCC TGCGCTCGGG CATCCGCATT
    GCCCAGGGGT CCTACTTCCG CGTCACTCTC CTCAAGGCCA CGCCCGTGCA GGTGGATGGT
    GAACCCTGGG TCCAGGCTCC CGGGCACCTG ATCATCTCAG CTGCGGGCCC TAAGGTCCAC
    ATGCTCAGGA AGGCGAAGCA GAAGCCCAGG AAGGCGGGGC CCGCCAAGGA TGCACGAGCA
    GATGGGGCGC CGGCCCCTGA GGGGGACCCC AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848981.1
    CDS114..2789
    Translation

    Target ORF information:

    RefSeq Version XM_024993213.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase theta (DGKQ), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993213.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG AGGACGGGGA GGACAGCGCC
    GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG
    ACTCTGAAGA TCTTTGACGG CAGCGATGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG
    GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC
    TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC
    ACCGCGGCCG CGGGAAGGGT CTGGAGCACT GGGCCCGCTG AGGAGGAGGG CGGCAGAGCG
    GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC
    CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT
    GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG
    GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCA
    GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG
    AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT
    GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC
    GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG
    GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA
    GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG
    GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC
    CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTTAACCCCC ACCAGGTCTT TGAGCTGACC
    AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG
    GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCCG CCCTGGAGGA CCTGCGGCAC
    CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG
    GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTCTC CGTGCTGCTG
    TCAGTGGACG AGGCGGATGC CGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC
    GAGTCTGCCT GTGGGGAGGA CAGCGAGGCA GACGCAGAGC CCCCCAAGAT CGTGCAGATG
    AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG
    GAGGAGGAGC CCGGCAAGTT CACAAGCAGC TGGGGCTCAG GGGCCGACCT GTGGGGTTCC
    GACAGCGACT CGAGGTTCGA GAAGCCGCGC ATGGACGACG GGCTGCTGGA GGTGGTGGGG
    GTGACGGGCG TTGTGCACAT GGGCCAGGTC CAGAGCGGCC TGCGCTCGGG CATCCGCATT
    GCCCAGGGGT CCTACTTCCG CGTCACTCTC CTCAAGGCCA CGCCCGTGCA GGTGGATGGT
    GAACCCTGGG TCCAGGCTCC CGGGCACCTG ATCATCTCAG CTGCGGGCCC TAAGGTCCAC
    ATGCTCAGGA AGGCGAAGCA GAAGCCCAGG AAGGCGGGGC CCGCCAAGGA TGCACGAGCA
    GATGGGGCGC CGGCCCCTGA GGGGGACCCC AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01927
    Clone ID Related Accession (Same CDS sequence) NM_001206305.1
    Accession Version NM_001206305.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2012-08-31
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase theta
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03042595.1 and AAFC03021705.1. On Apr 20, 2011 this sequence version replaced XM_872918.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GTGAGTGGGG CGGCGGGGAG
    GGCGCCGACG GCAGCGTCCC CGCTGGCCCG GGCCGAGAGG TGGGGACGCC TGAGTCCGGC
    AAGCAGACTC TGAAGATCTT TGACGGCAGC GATGCGATGC AGCGAAACCA CTTCCGTGCA
    GTCACGGTCC CGCGCCTGGC CCGGAGCCAG GAGGTGCTGG AGGCGGCTCT GCGGGCTTAC
    TACATCTCCG AGGACCCTCA GGGCTTCCAG CTGCAGGCAC TCCCCACGCC TGTGCAGCTT
    GGCGACACCG CGGCCGCGGG AAGGGTCTGG AGCGCTGGGC CCGCTGAGGA GGAGGGCGGC
    AGAGCGGCTC CCGAGGCCTG GGTCCTCCGT GCTCTGCCCC GCACCCAGGA GATCCTGAAG
    ATCTACCCGG CCTGGCTCAA GGTGGGTGTG GCCTACGTGT CCATCCGTGT GACCCCGCAG
    AGCACTGCCC GCAGCGTGGT GCTGGAGGTC CTCCCGCTGC TCGGACGGCA GGCCGAGGGG
    GCCGAGGGCT TCCAGCTGGT GGAGGTGCTC ATGGGCAGCA GACAAGTCCA GCGCACGGTG
    CTGGCAGACG AGGAGCCCTT GCTTGACCGG CTTCGTGAGA TCCGGCAGAC GTCCCTGCGG
    CAGATGAGCC AGACGCGGTT CTACGTGGCT GAGAGCAGAG TGCCGGCCCC GCGCGTCTCT
    CTGTTTGTGG GCGGTCTGCC GCCAGGCCTG TCCCCCCAGG AGTACCGCAG GTTGCTGGAT
    GAGGCCGTTA CCAGCAAAGC TGCCCTGGTG ACCGTGAGCC ACGTGTATTC CGCCCAAGGT
    GCCGTGGTGC TGGACGTGGC ATGCTTTGCA GAGGCCGAGC GGCTGTACAT GCTGATCAGG
    GACACAGCTG TGCGTGGCCG GCCACTGACT GCCCTGGTGC TCCCGGAGGT GCTGCACACG
    AAGCTGGCCC CAGACTGCCG CCCCCTGCTC GTGTTTGTGA ACCCCAGGAG TGGAGGACTC
    AAGGGCCGCG ACCTGCTCTG CAGTTTCCGG AAGCTGCTCA ACCCCCACCA GGTCTTTGAG
    CTGACCAACG GGGGGCCGCT GCCTGGGTTC CACGTGTTCT CCCGAGTGCC CTGCTTCCGG
    GTGCTGGTGT GCGGCGGGGA CGGCACTGTG GGCTGGGTAC TCGCCGCCCT GGAGGACCTG
    CGGCACCACC TGGCCTGCCC GGAGCCTGCT GTAGCCATCC TGCCCCTGGG CACAGGGAAC
    GACCTGGGCC GGGTCCTGCG CTGGGGCGCG GGCTACAGCG GGGAGGACCC GTTCTCCGTG
    CTGCTGTCAG TGGACGAGGC GGATGCCGTG CTCGTGGACC GCTGGACCAT CCTGCTGGAC
    GCTCACGAGT CTGCCTGTGG GGAGGACAGC GAGGCAGACG CAGAGCCCCC CAAGATCGTG
    CAGATGAGTA ACTACTGTGG GATCGGCATC GACGCAGAGC TGAGCCTGGA CTTCCACCAG
    GCGCGGGAGG AGGAGCCCGG CAAGTTCACA AGCAGGTTCC ACAACAAGGG CGTGTATGTG
    CGGGTCGGGC TGCAGAAGAT CAGCCACTCC CGCAGCCTGC ACAGGGCCCT GCGGCTCCAG
    GTGGAGCAGC AGGAGGTGGA GCTGCCCAGC ATCGAGGGGC TCATCTTCAT CAACATCCCC
    AGCTGGGGCT CAGGGGCCGA CCTGTGGGGT TCCGACAGCG ACTCGAGGTT CGAGAAGCCG
    CGCATGGACG ACGGGCTGCT GGAGGTGGTG GGGGTGACGG GCGTTGTGCA CATGGGCCAG
    GTCCAGAGCG GCCTGCGCTC GGGCATCCGC ATTGCCCAGG GGTCCTACTT CCGCGTCACT
    CTCCTCAAGG CCACGCCCGT GCAGGTGGAT GGTGAACCCT GGGTCCAGGC TCCCGGGCAC
    CTGATCATCT CAGCTGCGGG CCCTAAGGTC CACATGCTCA GGAAGGCGAA GCAGAAGCCC
    AGGAAGGCGG GGCCCGCCAA GGATGCACGA GCAGATGGGG CGCCGGCCCC TGAGGGGGAC
    CCCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193234.1
    CDS1..2829
    Translation

    Target ORF information:

    RefSeq Version NM_001206305.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase, theta 110kDa (DGKQ), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
    GGCAGCCCGA CCTCCAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG
    TCTGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG
    AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT
    TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG
    TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG
    GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG
    AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC
    TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC
    GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC
    TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC
    ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG
    CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT CAGCAAGATG
    CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GTGAGTGGGG CGGCGGGGAG
    GGCGCCGACG GCAGCGTCCC CGCTGGCCCG GGCCGAGAGG TGGGGACGCC TGAGTCCGGC
    AAGCAGACTC TGAAGATCTT TGACGGCAGC GATGCGATGC AGCGAAACCA CTTCCGTGCA
    GTCACGGTCC CGCGCCTGGC CCGGAGCCAG GAGGTGCTGG AGGCGGCTCT GCGGGCTTAC
    TACATCTCCG AGGACCCTCA GGGCTTCCAG CTGCAGGCAC TCCCCACGCC TGTGCAGCTT
    GGCGACACCG CGGCCGCGGG AAGGGTCTGG AGCGCTGGGC CCGCTGAGGA GGAGGGCGGC
    AGAGCGGCTC CCGAGGCCTG GGTCCTCCGT GCTCTGCCCC GCACCCAGGA GATCCTGAAG
    ATCTACCCGG CCTGGCTCAA GGTGGGTGTG GCCTACGTGT CCATCCGTGT GACCCCGCAG
    AGCACTGCCC GCAGCGTGGT GCTGGAGGTC CTCCCGCTGC TCGGACGGCA GGCCGAGGGG
    GCCGAGGGCT TCCAGCTGGT GGAGGTGCTC ATGGGCAGCA GACAAGTCCA GCGCACGGTG
    CTGGCAGACG AGGAGCCCTT GCTTGACCGG CTTCGTGAGA TCCGGCAGAC GTCCCTGCGG
    CAGATGAGCC AGACGCGGTT CTACGTGGCT GAGAGCAGAG TGCCGGCCCC GCGCGTCTCT
    CTGTTTGTGG GCGGTCTGCC GCCAGGCCTG TCCCCCCAGG AGTACCGCAG GTTGCTGGAT
    GAGGCCGTTA CCAGCAAAGC TGCCCTGGTG ACCGTGAGCC ACGTGTATTC CGCCCAAGGT
    GCCGTGGTGC TGGACGTGGC ATGCTTTGCA GAGGCCGAGC GGCTGTACAT GCTGATCAGG
    GACACAGCTG TGCGTGGCCG GCCACTGACT GCCCTGGTGC TCCCGGAGGT GCTGCACACG
    AAGCTGGCCC CAGACTGCCG CCCCCTGCTC GTGTTTGTGA ACCCCAGGAG TGGAGGACTC
    AAGGGCCGCG ACCTGCTCTG CAGTTTCCGG AAGCTGCTCA ACCCCCACCA GGTCTTTGAG
    CTGACCAACG GGGGGCCGCT GCCTGGGTTC CACGTGTTCT CCCGAGTGCC CTGCTTCCGG
    GTGCTGGTGT GCGGCGGGGA CGGCACTGTG GGCTGGGTAC TCGCCGCCCT GGAGGACCTG
    CGGCACCACC TGGCCTGCCC GGAGCCTGCT GTAGCCATCC TGCCCCTGGG CACAGGGAAC
    GACCTGGGCC GGGTCCTGCG CTGGGGCGCG GGCTACAGCG GGGAGGACCC GTTCTCCGTG
    CTGCTGTCAG TGGACGAGGC GGATGCCGTG CTCGTGGACC GCTGGACCAT CCTGCTGGAC
    GCTCACGAGT CTGCCTGTGG GGAGGACAGC GAGGCAGACG CAGAGCCCCC CAAGATCGTG
    CAGATGAGTA ACTACTGTGG GATCGGCATC GACGCAGAGC TGAGCCTGGA CTTCCACCAG
    GCGCGGGAGG AGGAGCCCGG CAAGTTCACA AGCAGGTTCC ACAACAAGGG CGTGTATGTG
    CGGGTCGGGC TGCAGAAGAT CAGCCACTCC CGCAGCCTGC ACAGGGCCCT GCGGCTCCAG
    GTGGAGCAGC AGGAGGTGGA GCTGCCCAGC ATCGAGGGGC TCATCTTCAT CAACATCCCC
    AGCTGGGGCT CAGGGGCCGA CCTGTGGGGT TCCGACAGCG ACTCGAGGTT CGAGAAGCCG
    CGCATGGACG ACGGGCTGCT GGAGGTGGTG GGGGTGACGG GCGTTGTGCA CATGGGCCAG
    GTCCAGAGCG GCCTGCGCTC GGGCATCCGC ATTGCCCAGG GGTCCTACTT CCGCGTCACT
    CTCCTCAAGG CCACGCCCGT GCAGGTGGAT GGTGAACCCT GGGTCCAGGC TCCCGGGCAC
    CTGATCATCT CAGCTGCGGG CCCTAAGGTC CACATGCTCA GGAAGGCGAA GCAGAAGCCC
    AGGAAGGCGG GGCCCGCCAA GGATGCACGA GCAGATGGGG CGCCGGCCCC TGAGGGGGAC
    CCCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.