TSHZ2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSHZ2
Entrez Gene ID	515677
Full Name	teashirt zinc finger homeobox 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010822637.2	XP_010820939.1	teashirt homolog 2 isoform X2
XM_005215074.3	XP_005215131.2	teashirt homolog 2 isoform X1
XM_005194469.3	XP_005194526.2	teashirt homolog 2 isoform X1
XM_010811771.3	XP_010810073.2	teashirt homolog 2 isoform X2
XM_593742.7	XP_593742.6	teashirt homolog 2 isoform X1
XM_002692360.5	XP_002692406.4	teashirt homolog 2 isoform X1
XM_010811771.2	XP_010810073.1	teashirt homolog 2 isoform X2
XM_005215074.4	XP_005215131.3	teashirt homolog 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tshz2	XP_006235721.1
Xenopus tropicalis (tropical clawed frog)	tshz2	XP_002935441.2
Danio rerio (zebrafish)	tshz2	XP_005174183.1
Homo sapiens (human)	TSHZ2	NP_775756.3
Canis lupus familiaris (dog)	TSHZ2	XP_003433270.1
Bos taurus (cattle)	TSHZ2	XP_002692406.3
Pan troglodytes (chimpanzee)	TSHZ2	XP_514730.2
	TSHZ2	XP_001097666.1
Mus musculus (house mouse)	Tshz2	NP_536703.2
Gallus gallus (chicken)	TSHZ2	XP_004947311.1

Gene Ontology

The following TSHZ2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSHZ2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222139	XM_010811771.3 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa222138	XM_005215074.4 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa49387	XM_593742.7 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa49387	XM_002692360.5 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa49387	XM_005215074.3 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa49387	XM_005194469.3 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa49388	XM_010811771.2 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa49388	XM_010822637.2 Latest version!	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSHZ2 ( XM_010811771.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810073.2 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa222139
Clone ID Related Accession (Same CDS sequence)	XM_010811771.3
Accession Version	XM_010811771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010811771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAGCAGCA GGCACCCAAG CGGGCGGCAG GCTATGCCCA GGAGGAACAG 
CTGAAGGAAG AGGAGGAAAT CAAAGAGGAA GAGGAGGAAG AGGAAGACAG TGGTTCGGGA 
GCTCAACCTC AGGGCAGCAA TGACGCAGGG ACAGACGAGG AGCTGGAAAC AGGCCCTGAG 
CAAAAGGGCT GCTTCAGCTA CCAGAACTCT CCAGGAAGCC ACCTGTCCAA CCAGGACGCT 
GAGAACGAGT CCCTGCTGAG CGATGCCAGT GATCAGGTGT CAGACATCAA GAGCGTGTGC 
GGCCGAGATG CCTCGGATAA GAAAGCCAGC GTGCACCCTA AGCTTCCAAA CGAAGCCCAC 
AGCTGCATGG ATAAGATGAC CGCCGTCTAC GCCAACATCT TGTCTGATTC CTACTGGTCG 
GGCCTGGGTC TTGGCTTCAA GCTGTCCAAC AGCGAGAGGC GGAATTGTGA CACCCGAAAC 
GGCGGCAACA AGACGGATTT CGACTGGCAC CAAGACGCTC TGTCCAAAAG CCTGCAGCAG 
AACTTGCCCT CGAGGTCCGT GTCGAAGCCC AGCCTGTTCA GCTCGGTCCA GCTGTACCGG 
CAGAGCAGCA AAATGTGCGG GACTGTTTTC ACCGGGGCCA GCAGGTTCCG GTGCCGGCAG 
TGCAGCGCGG CCTACGACAC CCTGGTTGAG CTGACCGTCC ACATGAACGA AACCGGCCAC 
TACCAAGACG ACAACCGCAA AAAGGACAAG CTCAGGCCCA CGAGCTACTC CAAGCCCCGA 
AAGAGGGCTT TCCAGGATAT GGACAAGGAG GATGCTCAAA AGGTCCTGAA ATGCATGTTT 
TGCGGTGATT CCTTCGATTC CCTCCAAGAT TTGAGTGTCC ACATGATCAA AACAAAACAT 
TACCAAAAAG TGCCTTTGAA GGAGCCAGTC CCAACCATTT CATCAAAAAT GGTCACTCCC 
GCCAAGAAAC GCGTCTTCGA CGTCAATCGG CCATGCTCCC CGGATTCAAC CACTGGGTCG 
TTTGCAGATT CGTTTCCGCC CCAGAAGAAC GCCAACCTCC AACTGTCCTG CAACAACCGC 
TACGGCTACC AAAACGGTGC CAGCTACACC TGGCAGTTCG AGGCCTGCAA GTCCCAGATC 
TTGAAGTGCA TGGAGTGTGG GAGCTCCCAC GACACTCTGC AGCAGCTCAC CACCCACATG 
ATGGTCACCG GTCACTTTCT CAAGGTCACC AGCTCCGCCT CCAAGAAAGG CAAGCAGCTG 
GTGCTGGACC CACTGGCTGT GGAGAAGATG CAGTCATTGT CAGATGCCCC AAACAGTGAT 
GCCCTGGCTC CCAAGCCATC AAGTAACTCT GCCTCTGATG CCGCAGCCTC CTCGACGGAG 
CTGAAGAAGG ACAGCAAAAA GGAAAAGCCA GAGGAGATCA ACAAGGATGA GAAAGTCATG 
AAAAGCGAGG ACTATGAAGA CCCTCTCCAA AAGCCACTAG ATCCTACGAT AAAGTACCAG 
TATCTGAGGG AGGAGGACTT GGAAGATGGC TCGAAGGGCG GAGGGGACAT TTTGAAGTCT 
TTGGAAAATA CTGTTACCAC CGCCATCAAC AAAGCCCAGA ATGGGGCTCC CAGCTGGAGC 
GCGTACCCCA GCATCCACGC CGCCTACCAG CTCTCGGAGG GCACCAAGCC TTCCTTGCCT 
ATGGGCTCCC AAGTCCTTCA GATCCGACCC AACCTCAGCA ACAAGCTGAG GCCGATCGCA 
CCCAAGTGGA AAGTGATGCC CCTGGTCTCC GTGCCTGCAC ACCTGGCCCC TTACACGCAA 
GTGAAGAAGG AGCTGACGGA CAAAGAGGAA GCGGTGAGGG AGTGTGGGAA AGAAAGTCCC 
TGTGAGGAGG CGTCCTCGTT CAGCCACAGC GAGGGGCATG CTTTCTGCAA AAGCGAACCC 
CCTGCGGAGT CTAAAAAGGT TGAGCCGCGT CCCCCGAAGG AGGAGGACAA GCTGATGAGA 
GAGGGCAGTG AGAAAGAGAA AGCCCCGGCT TTGGAGCCAG CGTCTCCACT CAGCAACGGC 
TGCGCCATCG CCAACCCTGC AGCGGCCCTG CCGGGCATCA ACCCACTCAG CGCCCTGCAG 
TCTGTCCTGA ACAATCACCT GGGCAAAGCC ACAGAACCCT TGCGCACGCC CTCCTGCTCC 
AGCCCAAGCT CAAGCACAAT GTCCATGTTT CACAAGCCGA GCCTCAGCGT CATGGACAAG 
CCAGTCCTGA GTCCCCCCTC CACGAGGCCG GCCAGCGTGT CCAGACGCTA TCTGTTCGAG 
AACAACGATC AGCCCATCGA CCTGACCAAG TCCAAGAGCA AGAAGGCCGA GTCCTCGCAA 
GCACAGTCCT GCACGTCCCC GCCCCAGAAG CACGCCCTGT CCGACATCGC CGACATGGTC 
AAGGTCCTCC CCAAGGCCAC CACCCCGAAG CCCGCCACCT CCCCCAGGGT CCCTCCCGTG 
AAGCTGGAGA TGGATGTCAG GCGCTTCGAG GACGTGTCCA GCGAGGTCTC CACCTTGCAT 
AAGAGGAAAG GCCGGCAGTC CAACTGGAAC CCCCAGCATC TTCTGATCCT GCAGGCTCAG 
TTTGCCTCCA GCCTCTTCCA GACCTCCGAG GGCAAATACC TGCTGTCCGA CCTGGGCCCG 
CAAGAGCGGA TGCAGATCTC GAAGTTTACG GGACTCTCGA TGACCACCAT CAGCCACTGG 
CTCGCCAACG TCAAGTACCA GCTTAGGAAA ACGGGCGGGA CCAAGTTCCT GAAAAACATG 
GACAAAGGCC ACCCCATCTT CTACTGCAGT GACTGTGCCT CCCAGTTCCG AACCCCGTCT 
ACCTACATCA GCCACTTAGA GTCCCACCTG GGCTTCCAGA TGAAGGACAT GACCCGCCTG 
GCTGTGGAGC AGCAAGGCAA AGCGGAGCAG GAGATCTCCC GGGTGTCGTC GGCTCAGCGG 
TCCCCGGAAA GCACAGCTGG CGAAGAGGAC ACGGACTCTA AATTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810073.2
CDS	825..3815
Translation	MPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFK

Target ORF information:

RefSeq Version	XM_010811771.3
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811771.3

ORF Insert Sequence:

ATGCCAAGGA GGAAGCAGCA GGCACCCAAG CGGGCGGCAG GCTATGCCCA GGAGGAACAG 
CTGAAGGAAG AGGAGGAAAT CAAAGAGGAA GAGGAGGAAG AGGAAGACAG TGGTTCGGGA 
GCTCAACCTC AGGGCAGCAA TGACGCAGGG ACAGACGAGG AGCTGGAAAC AGGCCCTGAG 
CAAAAGGGCT GCTTCAGCTA CCAGAACTCT CCAGGAAGCC ACCTGTCCAA CCAGGACGCT 
GAGAACGAGT CCCTGCTGAG CGATGCCAGT GATCAGGTGT CAGACATCAA GAGCGTGTGC 
GGCCGAGATG CCTCGGATAA GAAAGCCAGC GTGCACCCTA AGCTTCCAAA CGAAGCCCAC 
AGCTGCATGG ATAAGATGAC CGCCGTCTAC GCCAACATCT TGTCTGATTC CTACTGGTCG 
GGCCTGGGTC TTGGCTTCAA GCTGTCCAAC AGCGAGAGGC GGAATTGTGA CACCCGAAAC 
GGCGGCAACA AGACGGATTT CGACTGGCAC CAAGACGCTC TGTCCAAAAG CCTGCAGCAG 
AACTTGCCCT CGAGGTCCGT GTCGAAGCCC AGCCTGTTCA GCTCGGTCCA GCTGTACCGG 
CAGAGCAGCA AAATGTGCGG GACTGTTTTC ACCGGGGCCA GCAGGTTCCG GTGCCGGCAG 
TGCAGCGCGG CCTACGACAC CCTGGTTGAG CTGACCGTCC ACATGAACGA AACCGGCCAC 
TACCAAGACG ACAACCGCAA AAAGGACAAG CTCAGGCCCA CGAGCTACTC CAAGCCCCGA 
AAGAGGGCTT TCCAGGATAT GGACAAGGAG GATGCTCAAA AGGTCCTGAA ATGCATGTTT 
TGCGGTGATT CCTTCGATTC CCTCCAAGAT TTGAGTGTCC ACATGATCAA AACAAAACAT 
TACCAAAAAG TGCCTTTGAA GGAGCCAGTC CCAACCATTT CATCAAAAAT GGTCACTCCC 
GCCAAGAAAC GCGTCTTCGA CGTCAATCGG CCATGCTCCC CGGATTCAAC CACTGGGTCG 
TTTGCAGATT CGTTTCCGCC CCAGAAGAAC GCCAACCTCC AACTGTCCTG CAACAACCGC 
TACGGCTACC AAAACGGTGC CAGCTACACC TGGCAGTTCG AGGCCTGCAA GTCCCAGATC 
TTGAAGTGCA TGGAGTGTGG GAGCTCCCAC GACACTCTGC AGCAGCTCAC CACCCACATG 
ATGGTCACCG GTCACTTTCT CAAGGTCACC AGCTCCGCCT CCAAGAAAGG CAAGCAGCTG 
GTGCTGGACC CACTGGCTGT GGAGAAGATG CAGTCATTGT CAGATGCCCC AAACAGTGAT 
GCCCTGGCTC CCAAGCCATC AAGTAACTCT GCCTCTGATG CCGCAGCCTC CTCGACGGAG 
CTGAAGAAGG ACAGCAAAAA GGAAAAGCCA GAGGAGATCA ACAAGGATGA GAAAGTCATG 
AAAAGCGAGG ACTATGAAGA CCCTCTCCAA AAGCCACTAG ATCCTACGAT AAAGTACCAG 
TATCTGAGGG AGGAGGACTT GGAAGATGGC TCGAAGGGCG GAGGGGACAT TTTGAAGTCT 
TTGGAAAATA CTGTTACCAC CGCCATCAAC AAAGCCCAGA ATGGGGCTCC CAGCTGGAGC 
GCGTACCCCA GCATCCACGC CGCCTACCAG CTCTCGGAGG GCACCAAGCC TTCCTTGCCT 
ATGGGCTCCC AAGTCCTTCA GATCCGACCC AACCTCAGCA ACAAGCTGAG GCCGATCGCA 
CCCAAGTGGA AAGTGATGCC CCTGGTCTCC GTGCCTGCAC ACCTGGCCCC TTACACGCAA 
GTGAAGAAGG AGCTGACGGA CAAAGAGGAA GCGGTGAGGG AGTGTGGGAA AGAAAGTCCC 
TGTGAGGAGG CGTCCTCGTT CAGCCACAGC GAGGGGCATG CTTTCTGCAA AAGCGAACCC 
CCTGCGGAGT CTAAAAAGGT TGAGCCGCGT CCCCCGAAGG AGGAGGACAA GCTGATGAGA 
GAGGGCAGTG AGAAAGAGAA AGCCCCGGCT TTGGAGCCAG CGTCTCCACT CAGCAACGGC 
TGCGCCATCG CCAACCCTGC AGCGGCCCTG CCGGGCATCA ACCCACTCAG CGCCCTGCAG 
TCTGTCCTGA ACAATCACCT GGGCAAAGCC ACAGAACCCT TGCGCACGCC CTCCTGCTCC 
AGCCCAAGCT CAAGCACAAT GTCCATGTTT CACAAGCCGA GCCTCAGCGT CATGGACAAG 
CCAGTCCTGA GTCCCCCCTC CACGAGGCCG GCCAGCGTGT CCAGACGCTA TCTGTTCGAG 
AACAACGATC AGCCCATCGA CCTGACCAAG TCCAAGAGCA AGAAGGCCGA GTCCTCGCAA 
GCACAGTCCT GCACGTCCCC GCCCCAGAAG CACGCCCTGT CCGACATCGC CGACATGGTC 
AAGGTCCTCC CCAAGGCCAC CACCCCGAAG CCCGCCACCT CCCCCAGGGT CCCTCCCGTG 
AAGCTGGAGA TGGATGTCAG GCGCTTCGAG GACGTGTCCA GCGAGGTCTC CACCTTGCAT 
AAGAGGAAAG GCCGGCAGTC CAACTGGAAC CCCCAGCATC TTCTGATCCT GCAGGCTCAG 
TTTGCCTCCA GCCTCTTCCA GACCTCCGAG GGCAAATACC TGCTGTCCGA CCTGGGCCCG 
CAAGAGCGGA TGCAGATCTC GAAGTTTACG GGACTCTCGA TGACCACCAT CAGCCACTGG 
CTCGCCAACG TCAAGTACCA GCTTAGGAAA ACGGGCGGGA CCAAGTTCCT GAAAAACATG 
GACAAAGGCC ACCCCATCTT CTACTGCAGT GACTGTGCCT CCCAGTTCCG AACCCCGTCT 
ACCTACATCA GCCACTTAGA GTCCCACCTG GGCTTCCAGA TGAAGGACAT GACCCGCCTG 
GCTGTGGAGC AGCAAGGCAA AGCGGAGCAG GAGATCTCCC GGGTGTCGTC GGCTCAGCGG 
TCCCCGGAAA GCACAGCTGG CGAAGAGGAC ACGGACTCTA AATTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_005215074.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215131.3 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222138
Clone ID Related Accession (Same CDS sequence)	XM_005215074.4
Accession Version	XM_005215074.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215074.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAGCAGCA GGCACCCAAG CGGGCGGCAG GCTATGCCCA GGAGGAACAG 
CTGAAGGAAG AGGAGGAAAT CAAAGAGGAA GAGGAGGAAG AGGAAGACAG TGGTTCGGGA 
GCTCAACCTC AGGGCAGCAA TGACGCAGGG ACAGACGAGG AGCTGGAAAC AGGCCCTGAG 
CAAAAGGGCT GCTTCAGCTA CCAGAACTCT CCAGGAAGCC ACCTGTCCAA CCAGGACGCT 
GAGAACGAGT CCCTGCTGAG CGATGCCAGT GATCAGGTGT CAGACATCAA GAGCGTGTGC 
GGCCGAGATG CCTCGGATAA GAAAGCCAGC GTGCACCCTA AGCTTCCAAA CGAAGCCCAC 
AGCTGCATGG ATAAGATGAC CGCCGTCTAC GCCAACATCT TGTCTGATTC CTACTGGTCG 
GGCCTGGGTC TTGGCTTCAA GCTGTCCAAC AGCGAGAGGC GGAATTGTGA CACCCGAAAC 
GGCGGCAACA AGACGGATTT CGACTGGCAC CAAGACGCTC TGTCCAAAAG CCTGCAGCAG 
AACTTGCCCT CGAGGTCCGT GTCGAAGCCC AGCCTGTTCA GCTCGGTCCA GCTGTACCGG 
CAGAGCAGCA AAATGTGCGG GACTGTTTTC ACCGGGGCCA GCAGGTTCCG GTGCCGGCAG 
TGCAGCGCGG CCTACGACAC CCTGGTTGAG CTGACCGTCC ACATGAACGA AACCGGCCAC 
TACCAAGACG ACAACCGCAA AAAGGACAAG CTCAGGCCCA CGAGCTACTC CAAGCCCCGA 
AAGAGGGCTT TCCAGGATAT GGACAAGGAG GATGCTCAAA AGGTCCTGAA ATGCATGTTT 
TGCGGTGATT CCTTCGATTC CCTCCAAGAT TTGAGTGTCC ACATGATCAA AACAAAACAT 
TACCAAAAAG TGCCTTTGAA GGAGCCAGTC CCAACCATTT CATCAAAAAT GGTCACTCCC 
GCCAAGAAAC GCGTCTTCGA CGTCAATCGG CCATGCTCCC CGGATTCAAC CACTGGGTCG 
TTTGCAGATT CGTTTCCGCC CCAGAAGAAC GCCAACCTCC AACTGTCCTG CAACAACCGC 
TACGGCTACC AAAACGGTGC CAGCTACACC TGGCAGTTCG AGGCCTGCAA GTCCCAGATC 
TTGAAGTGCA TGGAGTGTGG GAGCTCCCAC GACACTCTGC AGCAGCTCAC CACCCACATG 
ATGGTCACCG GTCACTTTCT CAAGGTCACC AGCTCCGCCT CCAAGAAAGG CAAGCAGCTG 
GTGCTGGACC CACTGGCTGT GGAGAAGATG CAGTCATTGT CAGATGCCCC AAACAGTGAT 
GCCCTGGCTC CCAAGCCATC AAGTAACTCT GCCTCTGATG CCGCAGCCTC CTCGACGGAG 
CTGAAGAAGG ACAGCAAAAA GGAAAAGCCA GAGGAGATCA ACAAGGATGA GAAAGTCATG 
AAAAGCGAGG ACTATGAAGA CCCTCTCCAA AAGCCACTAG ATCCTACGAT AAAGTACCAG 
TATCTGAGGG AGGAGGACTT GGAAGATGGC TCGAAGGGCG GAGGGGACAT TTTGAAGTCT 
TTGGAAAATA CTGTTACCAC CGCCATCAAC AAAGCCCAGA ATGGGGCTCC CAGCTGGAGC 
GCGTACCCCA GCATCCACGC CGCCTACCAG CTCTCGGAGG GCACCAAGCC TTCCTTGCCT 
ATGGGCTCCC AAGTCCTTCA GATCCGACCC AACCTCAGCA ACAAGCTGAG GCCGATCGCA 
CCCAAGTGGA AAGTGATGCC CCTGGTCTCC GTGCCTGCAC ACCTGGCCCC TTACACGCAA 
GTGAAGAAGG AGCTGACGGA CAAAGAGGAA GCGGTGAGGG AGTGTGGGAA AGAAAGTCCC 
TGTGAGGAGG CGTCCTCGTT CAGCCACAGC GAGGGGCATG CTTTCTGCAA AAGCGAACCC 
CCTGCGGAGT CTAAAAAGGT TGAGCCGCGT CCCCCGAAGG AGGAGGACAA GCTGATGAGA 
GAGGGCAGTG AGAAAGAGAA AGCCCCGGCT TTGGAGCCAG CGTCTCCACT CAGCAACGGC 
TGCGCCATCG CCAACCCTGC AGCGGCCCTG CCGGGCATCA ACCCACTCAG CGCCCTGCAG 
TCTGTCCTGA ACAATCACCT GGGCAAAGCC ACAGAACCCT TGCGCACGCC CTCCTGCTCC 
AGCCCAAGCT CAAGCACAAT GTCCATGTTT CACAAGCCGA GCCTCAGCGT CATGGACAAG 
CCAGTCCTGA GTCCCCCCTC CACGAGGCCG GCCAGCGTGT CCAGACGCTA TCTGTTCGAG 
AACAACGATC AGCCCATCGA CCTGACCAAG TCCAAGAGCA AGAAGGCCGA GTCCTCGCAA 
GCACAGTCCT GCACGTCCCC GCCCCAGAAG CACGCCCTGT CCGACATCGC CGACATGGTC 
AAGGTCCTCC CCAAGGCCAC CACCCCGAAG CCCGCCACCT CCCCCAGGGT CCCTCCCGTG 
AAGCTGGAGA TGGATGTCAG GCGCTTCGAG GACGTGTCCA GCGAGGTCTC CACCTTGCAT 
AAGAGGAAAG GCCGGCAGTC CAACTGGAAC CCCCAGCATC TTCTGATCCT GCAGGCTCAG 
TTTGCCTCCA GCCTCTTCCA GACCTCCGAG GGCAAATACC TGCTGTCCGA CCTGGGCCCG 
CAAGAGCGGA TGCAGATCTC GAAGTTTACG GGACTCTCGA TGACCACCAT CAGCCACTGG 
CTCGCCAACG TCAAGTACCA GCTTAGGAAA ACGGGCGGGA CCAAGTTCCT GAAAAACATG 
GACAAAGGCC ACCCCATCTT CTACTGCAGT GACTGTGCCT CCCAGTTCCG AACCCCGTCT 
ACCTACATCA GCCACTTAGA GTCCCACCTG GGCTTCCAGA TGAAGGACAT GACCCGCCTG 
GCTGTGGAGC AGCAAGGCAA AGCGGAGCAG GAGATCTCCC GGGTGTCGTC GGCTCAGCGG 
TCCCCGGAAA GCACAGCTGG CGAAGAGGAC ACGGACTCTA AATTCAAGTG TAAGTTGTGC 
TGCCGGACAT TTGTGAGCAA ACATGCGGTA AAACTCCACC TAAGCAAAAC GCACAGCAAG 
TCCCCCGAAC ACCACTCTCA GTTTGTAACA GACGCGGACG AAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215131.3
CDS	825..3932
Translation	MPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFKCKLCCRTFVSKHAVKLHLSKTHSKSPEHHSQFVTDADEE

Target ORF information:

RefSeq Version	XM_005215074.4
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215074.4

ORF Insert Sequence:

ATGCCAAGGA GGAAGCAGCA GGCACCCAAG CGGGCGGCAG GCTATGCCCA GGAGGAACAG 
CTGAAGGAAG AGGAGGAAAT CAAAGAGGAA GAGGAGGAAG AGGAAGACAG TGGTTCGGGA 
GCTCAACCTC AGGGCAGCAA TGACGCAGGG ACAGACGAGG AGCTGGAAAC AGGCCCTGAG 
CAAAAGGGCT GCTTCAGCTA CCAGAACTCT CCAGGAAGCC ACCTGTCCAA CCAGGACGCT 
GAGAACGAGT CCCTGCTGAG CGATGCCAGT GATCAGGTGT CAGACATCAA GAGCGTGTGC 
GGCCGAGATG CCTCGGATAA GAAAGCCAGC GTGCACCCTA AGCTTCCAAA CGAAGCCCAC 
AGCTGCATGG ATAAGATGAC CGCCGTCTAC GCCAACATCT TGTCTGATTC CTACTGGTCG 
GGCCTGGGTC TTGGCTTCAA GCTGTCCAAC AGCGAGAGGC GGAATTGTGA CACCCGAAAC 
GGCGGCAACA AGACGGATTT CGACTGGCAC CAAGACGCTC TGTCCAAAAG CCTGCAGCAG 
AACTTGCCCT CGAGGTCCGT GTCGAAGCCC AGCCTGTTCA GCTCGGTCCA GCTGTACCGG 
CAGAGCAGCA AAATGTGCGG GACTGTTTTC ACCGGGGCCA GCAGGTTCCG GTGCCGGCAG 
TGCAGCGCGG CCTACGACAC CCTGGTTGAG CTGACCGTCC ACATGAACGA AACCGGCCAC 
TACCAAGACG ACAACCGCAA AAAGGACAAG CTCAGGCCCA CGAGCTACTC CAAGCCCCGA 
AAGAGGGCTT TCCAGGATAT GGACAAGGAG GATGCTCAAA AGGTCCTGAA ATGCATGTTT 
TGCGGTGATT CCTTCGATTC CCTCCAAGAT TTGAGTGTCC ACATGATCAA AACAAAACAT 
TACCAAAAAG TGCCTTTGAA GGAGCCAGTC CCAACCATTT CATCAAAAAT GGTCACTCCC 
GCCAAGAAAC GCGTCTTCGA CGTCAATCGG CCATGCTCCC CGGATTCAAC CACTGGGTCG 
TTTGCAGATT CGTTTCCGCC CCAGAAGAAC GCCAACCTCC AACTGTCCTG CAACAACCGC 
TACGGCTACC AAAACGGTGC CAGCTACACC TGGCAGTTCG AGGCCTGCAA GTCCCAGATC 
TTGAAGTGCA TGGAGTGTGG GAGCTCCCAC GACACTCTGC AGCAGCTCAC CACCCACATG 
ATGGTCACCG GTCACTTTCT CAAGGTCACC AGCTCCGCCT CCAAGAAAGG CAAGCAGCTG 
GTGCTGGACC CACTGGCTGT GGAGAAGATG CAGTCATTGT CAGATGCCCC AAACAGTGAT 
GCCCTGGCTC CCAAGCCATC AAGTAACTCT GCCTCTGATG CCGCAGCCTC CTCGACGGAG 
CTGAAGAAGG ACAGCAAAAA GGAAAAGCCA GAGGAGATCA ACAAGGATGA GAAAGTCATG 
AAAAGCGAGG ACTATGAAGA CCCTCTCCAA AAGCCACTAG ATCCTACGAT AAAGTACCAG 
TATCTGAGGG AGGAGGACTT GGAAGATGGC TCGAAGGGCG GAGGGGACAT TTTGAAGTCT 
TTGGAAAATA CTGTTACCAC CGCCATCAAC AAAGCCCAGA ATGGGGCTCC CAGCTGGAGC 
GCGTACCCCA GCATCCACGC CGCCTACCAG CTCTCGGAGG GCACCAAGCC TTCCTTGCCT 
ATGGGCTCCC AAGTCCTTCA GATCCGACCC AACCTCAGCA ACAAGCTGAG GCCGATCGCA 
CCCAAGTGGA AAGTGATGCC CCTGGTCTCC GTGCCTGCAC ACCTGGCCCC TTACACGCAA 
GTGAAGAAGG AGCTGACGGA CAAAGAGGAA GCGGTGAGGG AGTGTGGGAA AGAAAGTCCC 
TGTGAGGAGG CGTCCTCGTT CAGCCACAGC GAGGGGCATG CTTTCTGCAA AAGCGAACCC 
CCTGCGGAGT CTAAAAAGGT TGAGCCGCGT CCCCCGAAGG AGGAGGACAA GCTGATGAGA 
GAGGGCAGTG AGAAAGAGAA AGCCCCGGCT TTGGAGCCAG CGTCTCCACT CAGCAACGGC 
TGCGCCATCG CCAACCCTGC AGCGGCCCTG CCGGGCATCA ACCCACTCAG CGCCCTGCAG 
TCTGTCCTGA ACAATCACCT GGGCAAAGCC ACAGAACCCT TGCGCACGCC CTCCTGCTCC 
AGCCCAAGCT CAAGCACAAT GTCCATGTTT CACAAGCCGA GCCTCAGCGT CATGGACAAG 
CCAGTCCTGA GTCCCCCCTC CACGAGGCCG GCCAGCGTGT CCAGACGCTA TCTGTTCGAG 
AACAACGATC AGCCCATCGA CCTGACCAAG TCCAAGAGCA AGAAGGCCGA GTCCTCGCAA 
GCACAGTCCT GCACGTCCCC GCCCCAGAAG CACGCCCTGT CCGACATCGC CGACATGGTC 
AAGGTCCTCC CCAAGGCCAC CACCCCGAAG CCCGCCACCT CCCCCAGGGT CCCTCCCGTG 
AAGCTGGAGA TGGATGTCAG GCGCTTCGAG GACGTGTCCA GCGAGGTCTC CACCTTGCAT 
AAGAGGAAAG GCCGGCAGTC CAACTGGAAC CCCCAGCATC TTCTGATCCT GCAGGCTCAG 
TTTGCCTCCA GCCTCTTCCA GACCTCCGAG GGCAAATACC TGCTGTCCGA CCTGGGCCCG 
CAAGAGCGGA TGCAGATCTC GAAGTTTACG GGACTCTCGA TGACCACCAT CAGCCACTGG 
CTCGCCAACG TCAAGTACCA GCTTAGGAAA ACGGGCGGGA CCAAGTTCCT GAAAAACATG 
GACAAAGGCC ACCCCATCTT CTACTGCAGT GACTGTGCCT CCCAGTTCCG AACCCCGTCT 
ACCTACATCA GCCACTTAGA GTCCCACCTG GGCTTCCAGA TGAAGGACAT GACCCGCCTG 
GCTGTGGAGC AGCAAGGCAA AGCGGAGCAG GAGATCTCCC GGGTGTCGTC GGCTCAGCGG 
TCCCCGGAAA GCACAGCTGG CGAAGAGGAC ACGGACTCTA AATTCAAGTG TAAGTTGTGC 
TGCCGGACAT TTGTGAGCAA ACATGCGGTA AAACTCCACC TAAGCAAAAC GCACAGCAAG 
TCCCCCGAAC ACCACTCTCA GTTTGTAACA GACGCGGACG AAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_005215074.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215131.2 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.

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CloneID	OBa49387
Clone ID Related Accession (Same CDS sequence)	XM_005215074.3 , XM_005194469.3 , XM_593742.7 , XM_002692360.5
Accession Version	XM_005215074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215131.2
CDS	585..4106
Translation	MFNKEIKCLNSHQKKKKKNQQKKPNQKKKISKKQKQKERGEKTKTKNPKPNKQTNKAKPTSTSNQPAQPPVSCLPRREGGLRPPVEELQGQGDRGRVTPWEPPGRRRRRPGEASETAGPRARRLGAGAPEVGLERTRAMPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFKCKLCCRTFVSKHAVKLHLSKTHSKSPEHHSQFVTDADEE

Target ORF information:

RefSeq Version	XM_005215074.3
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215074.3

ORF Insert Sequence:

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_005194469.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194526.2 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa49387
Clone ID Related Accession (Same CDS sequence)	XM_005215074.3 , XM_005194469.3 , XM_593742.7 , XM_002692360.5
Accession Version	XM_005194469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194469.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194526.2
CDS	585..4106
Translation	MFNKEIKCLNSHQKKKKKNQQKKPNQKKKISKKQKQKERGEKTKTKNPKPNKQTNKAKPTSTSNQPAQPPVSCLPRREGGLRPPVEELQGQGDRGRVTPWEPPGRRRRRPGEASETAGPRARRLGAGAPEVGLERTRAMPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFKCKLCCRTFVSKHAVKLHLSKTHSKSPEHHSQFVTDADEE

Target ORF information:

RefSeq Version	XM_005194469.3
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194469.3

ORF Insert Sequence:

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_593742.7 ) cDNA ORF clone, Bos taurus(cattle) -> XP_593742.6 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.

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CloneID	OBa49387
Clone ID Related Accession (Same CDS sequence)	XM_005215074.3 , XM_005194469.3 , XM_593742.7 , XM_002692360.5
Accession Version	XM_593742.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_593742.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_593742.6
CDS	585..4106
Translation	MFNKEIKCLNSHQKKKKKNQQKKPNQKKKISKKQKQKERGEKTKTKNPKPNKQTNKAKPTSTSNQPAQPPVSCLPRREGGLRPPVEELQGQGDRGRVTPWEPPGRRRRRPGEASETAGPRARRLGAGAPEVGLERTRAMPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFKCKLCCRTFVSKHAVKLHLSKTHSKSPEHHSQFVTDADEE

Target ORF information:

RefSeq Version	XM_593742.7
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_593742.7

ORF Insert Sequence:

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_002692360.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002692406.4 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.

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CloneID	OBa49387
Clone ID Related Accession (Same CDS sequence)	XM_005215074.3 , XM_005194469.3 , XM_593742.7 , XM_002692360.5
Accession Version	XM_002692360.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002692360.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002692406.4
CDS	585..4106
Translation	MFNKEIKCLNSHQKKKKKNQQKKPNQKKKISKKQKQKERGEKTKTKNPKPNKQTNKAKPTSTSNQPAQPPVSCLPRREGGLRPPVEELQGQGDRGRVTPWEPPGRRRRRPGEASETAGPRARRLGAGAPEVGLERTRAMPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFKCKLCCRTFVSKHAVKLHLSKTHSKSPEHHSQFVTDADEE

Target ORF information:

RefSeq Version	XM_002692360.5
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002692360.5

ORF Insert Sequence:

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGTAAGTT GTGCTGCCGG 
ACATTTGTGA GCAAACATGC GGTAAAACTC CACCTAAGCA AAACGCACAG CAAGTCCCCC 
GAACACCACT CTCAGTTTGT AACAGACGCG GACGAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_010822637.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820939.1 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X3, mRNA.

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CloneID	OBa49388
Clone ID Related Accession (Same CDS sequence)	XM_010822637.2 , XM_010811771.2
Accession Version	XM_010822637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820939.1
CDS	585..3989
Translation	MFNKEIKCLNSHQKKKKKNQQKKPNQKKKISKKQKQKERGEKTKTKNPKPNKQTNKAKPTSTSNQPAQPPVSCLPRREGGLRPPVEELQGQGDRGRVTPWEPPGRRRRRPGEASETAGPRARRLGAGAPEVGLERTRAMPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFK

Target ORF information:

RefSeq Version	XM_010822637.2
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822637.2

ORF Insert Sequence:

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHZ2 ( XM_010811771.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810073.1 Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X3, mRNA.

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CloneID	OBa49388
Clone ID Related Accession (Same CDS sequence)	XM_010822637.2 , XM_010811771.2
Accession Version	XM_010811771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	teashirt homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810073.1
CDS	585..3989
Translation	MFNKEIKCLNSHQKKKKKNQQKKPNQKKKISKKQKQKERGEKTKTKNPKPNKQTNKAKPTSTSNQPAQPPVSCLPRREGGLRPPVEELQGQGDRGRVTPWEPPGRRRRRPGEASETAGPRARRLGAGAPEVGLERTRAMPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEEDSGSGAQPQGSNDAGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRDASDKKASVHPKLPNEAHSCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNGGNKTDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCRQCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADSFPPQKNANLQLSCNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSDAPNSDALAPKPSSNSASDAAASSTELKKDSKKEKPEEINKDEKVMKSEDYEDPLQKPLDPTIKYQYLREEDLEDGSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPSLPMGSQVLQIRPNLSNKLRPIAPKWKVMPLVSVPAHLAPYTQVKKELTDKEEAVRECGKESPCEEASSFSHSEGHAFCKSEPPAESKKVEPRPPKEEDKLMREGSEKEKAPALEPASPLSNGCAIANPAAALPGINPLSALQSVLNNHLGKATEPLRTPSCSSPSSSTMSMFHKPSLSVMDKPVLSPPSTRPASVSRRYLFENNDQPIDLTKSKSKKAESSQAQSCTSPPQKHALSDIADMVKVLPKATTPKPATSPRVPPVKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLAVEQQGKAEQEISRVSSAQRSPESTAGEEDTDSKFK

Target ORF information:

RefSeq Version	XM_010811771.2
Organism	Bos taurus(cattle)
Definition	Bos taurus teashirt zinc finger homeobox 2 (TSHZ2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811771.2

ORF Insert Sequence:

ATGTTTAATA AAGAAATCAA GTGTCTCAAC AGTCACCAAA AAAAAAAAAA AAAAAACCAA 
CAAAAAAAAC CAAACCAAAA AAAAAAAATT TCCAAAAAGC AAAAACAAAA AGAGAGAGGA 
GAAAAAACAA AAACAAAAAA TCCAAAACCA AACAAACAAA CAAACAAGGC AAAACCAACC 
TCTACTTCAA ACCAGCCGGC ACAGCCACCC GTGTCTTGCC TGCCACGCAG AGAGGGGGGT 
CTCCGGCCCC CGGTGGAGGA GTTGCAGGGA CAGGGGGACA GAGGCCGAGT GACGCCCTGG 
GAGCCACCGG GCAGGAGGCG GAGGAGACCC GGAGAGGCGA GCGAGACAGC GGGCCCCCGC 
GCGCGGCGGC TCGGGGCTGG GGCGCCAGAA GTGGGACTGG AGCGAACTAG AGCGATGCCA 
AGGAGGAAGC AGCAGGCACC CAAGCGGGCG GCAGGCTATG CCCAGGAGGA ACAGCTGAAG 
GAAGAGGAGG AAATCAAAGA GGAAGAGGAG GAAGAGGAAG ACAGTGGTTC GGGAGCTCAA 
CCTCAGGGCA GCAATGACGC AGGGACAGAC GAGGAGCTGG AAACAGGCCC TGAGCAAAAG 
GGCTGCTTCA GCTACCAGAA CTCTCCAGGA AGCCACCTGT CCAACCAGGA CGCTGAGAAC 
GAGTCCCTGC TGAGCGATGC CAGTGATCAG GTGTCAGACA TCAAGAGCGT GTGCGGCCGA 
GATGCCTCGG ATAAGAAAGC CAGCGTGCAC CCTAAGCTTC CAAACGAAGC CCACAGCTGC 
ATGGATAAGA TGACCGCCGT CTACGCCAAC ATCTTGTCTG ATTCCTACTG GTCGGGCCTG 
GGTCTTGGCT TCAAGCTGTC CAACAGCGAG AGGCGGAATT GTGACACCCG AAACGGCGGC 
AACAAGACGG ATTTCGACTG GCACCAAGAC GCTCTGTCCA AAAGCCTGCA GCAGAACTTG 
CCCTCGAGGT CCGTGTCGAA GCCCAGCCTG TTCAGCTCGG TCCAGCTGTA CCGGCAGAGC 
AGCAAAATGT GCGGGACTGT TTTCACCGGG GCCAGCAGGT TCCGGTGCCG GCAGTGCAGC 
GCGGCCTACG ACACCCTGGT TGAGCTGACC GTCCACATGA ACGAAACCGG CCACTACCAA 
GACGACAACC GCAAAAAGGA CAAGCTCAGG CCCACGAGCT ACTCCAAGCC CCGAAAGAGG 
GCTTTCCAGG ATATGGACAA GGAGGATGCT CAAAAGGTCC TGAAATGCAT GTTTTGCGGT 
GATTCCTTCG ATTCCCTCCA AGATTTGAGT GTCCACATGA TCAAAACAAA ACATTACCAA 
AAAGTGCCTT TGAAGGAGCC AGTCCCAACC ATTTCATCAA AAATGGTCAC TCCCGCCAAG 
AAACGCGTCT TCGACGTCAA TCGGCCATGC TCCCCGGATT CAACCACTGG GTCGTTTGCA 
GATTCGTTTC CGCCCCAGAA GAACGCCAAC CTCCAACTGT CCTGCAACAA CCGCTACGGC 
TACCAAAACG GTGCCAGCTA CACCTGGCAG TTCGAGGCCT GCAAGTCCCA GATCTTGAAG 
TGCATGGAGT GTGGGAGCTC CCACGACACT CTGCAGCAGC TCACCACCCA CATGATGGTC 
ACCGGTCACT TTCTCAAGGT CACCAGCTCC GCCTCCAAGA AAGGCAAGCA GCTGGTGCTG 
GACCCACTGG CTGTGGAGAA GATGCAGTCA TTGTCAGATG CCCCAAACAG TGATGCCCTG 
GCTCCCAAGC CATCAAGTAA CTCTGCCTCT GATGCCGCAG CCTCCTCGAC GGAGCTGAAG 
AAGGACAGCA AAAAGGAAAA GCCAGAGGAG ATCAACAAGG ATGAGAAAGT CATGAAAAGC 
GAGGACTATG AAGACCCTCT CCAAAAGCCA CTAGATCCTA CGATAAAGTA CCAGTATCTG 
AGGGAGGAGG ACTTGGAAGA TGGCTCGAAG GGCGGAGGGG ACATTTTGAA GTCTTTGGAA 
AATACTGTTA CCACCGCCAT CAACAAAGCC CAGAATGGGG CTCCCAGCTG GAGCGCGTAC 
CCCAGCATCC ACGCCGCCTA CCAGCTCTCG GAGGGCACCA AGCCTTCCTT GCCTATGGGC 
TCCCAAGTCC TTCAGATCCG ACCCAACCTC AGCAACAAGC TGAGGCCGAT CGCACCCAAG 
TGGAAAGTGA TGCCCCTGGT CTCCGTGCCT GCACACCTGG CCCCTTACAC GCAAGTGAAG 
AAGGAGCTGA CGGACAAAGA GGAAGCGGTG AGGGAGTGTG GGAAAGAAAG TCCCTGTGAG 
GAGGCGTCCT CGTTCAGCCA CAGCGAGGGG CATGCTTTCT GCAAAAGCGA ACCCCCTGCG 
GAGTCTAAAA AGGTTGAGCC GCGTCCCCCG AAGGAGGAGG ACAAGCTGAT GAGAGAGGGC 
AGTGAGAAAG AGAAAGCCCC GGCTTTGGAG CCAGCGTCTC CACTCAGCAA CGGCTGCGCC 
ATCGCCAACC CTGCAGCGGC CCTGCCGGGC ATCAACCCAC TCAGCGCCCT GCAGTCTGTC 
CTGAACAATC ACCTGGGCAA AGCCACAGAA CCCTTGCGCA CGCCCTCCTG CTCCAGCCCA 
AGCTCAAGCA CAATGTCCAT GTTTCACAAG CCGAGCCTCA GCGTCATGGA CAAGCCAGTC 
CTGAGTCCCC CCTCCACGAG GCCGGCCAGC GTGTCCAGAC GCTATCTGTT CGAGAACAAC 
GATCAGCCCA TCGACCTGAC CAAGTCCAAG AGCAAGAAGG CCGAGTCCTC GCAAGCACAG 
TCCTGCACGT CCCCGCCCCA GAAGCACGCC CTGTCCGACA TCGCCGACAT GGTCAAGGTC 
CTCCCCAAGG CCACCACCCC GAAGCCCGCC ACCTCCCCCA GGGTCCCTCC CGTGAAGCTG 
GAGATGGATG TCAGGCGCTT CGAGGACGTG TCCAGCGAGG TCTCCACCTT GCATAAGAGG 
AAAGGCCGGC AGTCCAACTG GAACCCCCAG CATCTTCTGA TCCTGCAGGC TCAGTTTGCC 
TCCAGCCTCT TCCAGACCTC CGAGGGCAAA TACCTGCTGT CCGACCTGGG CCCGCAAGAG 
CGGATGCAGA TCTCGAAGTT TACGGGACTC TCGATGACCA CCATCAGCCA CTGGCTCGCC 
AACGTCAAGT ACCAGCTTAG GAAAACGGGC GGGACCAAGT TCCTGAAAAA CATGGACAAA 
GGCCACCCCA TCTTCTACTG CAGTGACTGT GCCTCCCAGT TCCGAACCCC GTCTACCTAC 
ATCAGCCACT TAGAGTCCCA CCTGGGCTTC CAGATGAAGG ACATGACCCG CCTGGCTGTG 
GAGCAGCAAG GCAAAGCGGA GCAGGAGATC TCCCGGGTGT CGTCGGCTCA GCGGTCCCCG 
GAAAGCACAG CTGGCGAAGA GGACACGGAC TCTAAATTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TSHZ2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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