KCP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCP
Entrez Gene ID	515624
Full Name	kielin/chordin-like protein
Synonyms	CRIM2,KCP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015463112.1	XP_015318598.1	kielin/chordin-like protein isoform X8
XM_015470728.1	XP_015326214.1	kielin/chordin-like protein isoform X7
XM_015470732.1	XP_015326218.1	kielin/chordin-like protein isoform X11
XM_015470734.1	XP_015326220.1	kielin/chordin-like protein isoform X13
XM_015470724.2	XP_015326210.2	kielin/chordin-like protein isoform X2
XM_024991151.1	XP_024846919.1	kielin/chordin-like protein isoform X5
XM_024991153.1	XP_024846921.1	kielin/chordin-like protein isoform X7
XM_015463107.1	XP_015318593.1	kielin/chordin-like protein isoform X3
XM_015463114.1	XP_015318600.1	kielin/chordin-like protein isoform X10
XM_015470721.1	XP_015326207.1	kielin/chordin-like protein isoform X1
XM_015470723.1	XP_015326209.1	kielin/chordin-like protein isoform X1
XM_015470724.1	XP_015326210.1	kielin/chordin-like protein isoform X2
XM_015470727.1	XP_015326213.1	kielin/chordin-like protein isoform X6
XM_024991149.1	XP_024846917.1	kielin/chordin-like protein isoform X1
XM_015470733.1	XP_015326219.1	kielin/chordin-like protein isoform X12
XM_015463104.1	XP_015318590.1	kielin/chordin-like protein isoform X1
XM_015463105.1	XP_015318591.1	kielin/chordin-like protein isoform X1
XM_015463108.1	XP_015318594.1	kielin/chordin-like protein isoform X4
XM_015463109.1	XP_015318595.1	kielin/chordin-like protein isoform X5
XM_015463110.1	XP_015318596.1	kielin/chordin-like protein isoform X6
XM_015463113.1	XP_015318599.1	kielin/chordin-like protein isoform X9
XM_015463116.1	XP_015318602.1	kielin/chordin-like protein isoform X12
XM_015470725.1	XP_015326211.1	kielin/chordin-like protein isoform X4
XM_015470729.1	XP_015326215.1	kielin/chordin-like protein isoform X8
XM_024991152.1	XP_024846920.1	kielin/chordin-like protein isoform X5
XM_015470732.2	XP_015326218.2	kielin/chordin-like protein isoform X9
XM_015470721.2	XP_015326207.2	kielin/chordin-like protein isoform X1
XM_024991148.1	XP_024846916.1	kielin/chordin-like protein isoform X1
XM_015470725.2	XP_015326211.2	kielin/chordin-like protein isoform X4
XM_010804494.2	XP_010802796.1	kielin/chordin-like protein isoform X3
XM_015463103.1	XP_015318589.1	kielin/chordin-like protein isoform X1
XM_015463115.1	XP_015318601.1	kielin/chordin-like protein isoform X11
XM_015470722.2	XP_015326208.2	kielin/chordin-like protein isoform X1
XM_015470723.2	XP_015326209.2	kielin/chordin-like protein isoform X1
XM_024991150.1	XP_024846918.1	kielin/chordin-like protein isoform X1
XM_015470728.2	XP_015326214.2	kielin/chordin-like protein isoform X6
XM_015470733.2	XP_015326219.2	kielin/chordin-like protein isoform X11
XM_015470722.1	XP_015326208.1	kielin/chordin-like protein isoform X1
XM_015470726.1	XP_015326212.1	kielin/chordin-like protein isoform X5
XM_015470730.1	XP_015326216.1	kielin/chordin-like protein isoform X9
XM_015470731.1	XP_015326217.1	kielin/chordin-like protein isoform X10
XM_015463106.1	XP_015318592.1	kielin/chordin-like protein isoform X2
XM_015463111.1	XP_015318597.1	kielin/chordin-like protein isoform X7
XM_015463117.1	XP_015318603.1	kielin/chordin-like protein isoform X13
XM_010804494.3	XP_010802796.2	kielin/chordin-like protein isoform X3
XM_015470731.2	XP_015326217.2	kielin/chordin-like protein isoform X8
XM_024991154.1	XP_024846922.1	kielin/chordin-like protein isoform X10
XM_015470734.2	XP_015326220.2	kielin/chordin-like protein isoform X12

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCP	NP_001129386.1
Bos taurus (cattle)	LOC515624	XP_002686994.2
Mus musculus (house mouse)	Kcp	NP_001025156.3
	KCP	XP_002803510.1
Canis lupus familiaris (dog)	KCP	XP_539384.3
Pan troglodytes (chimpanzee)	LOC463713	XP_003318829.1
Rattus norvegicus (Norway rat)	Kcp	XP_231561.6

Gene Ontology

The following KCP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa234037	XM_015470733.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa234038	XM_015470734.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa89773	XM_015463116.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa89773	XM_015470733.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa89774	XM_015470734.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa89774	XM_015463117.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa234034	XM_015470731.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa234035	XM_015470732.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa234036	XM_024991154.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa89769	XM_015470729.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89769	XM_015463112.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89770	XM_015463113.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89770	XM_015470730.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89771	XM_015470731.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa89771	XM_015463114.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa89772	XM_015463115.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa89772	XM_015470732.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa234027	XM_024991149.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234027	XM_015470723.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234027	XM_024991148.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234027	XM_015470721.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234027	XM_015470722.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234027	XM_024991150.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234028	XM_015470724.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234029	XM_010804494.3 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234030	XM_015470725.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234031	XM_024991152.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa234031	XM_024991151.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa234032	XM_015470728.2 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa234033	XM_024991153.1 Latest version!	Bos taurus kielin/chordin-like protein (KCP), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa89763	XM_015463103.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89763	XM_015470723.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89763	XM_015463104.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89763	XM_015463105.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89763	XM_015470721.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89763	XM_015470722.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89764	XM_015463106.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89764	XM_015470724.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89765	XM_015463108.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89765	XM_015470725.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89766	XM_015470726.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89766	XM_015463109.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89767	XM_015463110.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89767	XM_015470727.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89768	XM_015463111.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89768	XM_015470728.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa49358	XM_015463107.1 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49358	XM_010804494.2 Latest version!	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCP ( XM_015470733.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326219.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa234037
Clone ID Related Accession (Same CDS sequence)	XM_015470733.2
Accession Version	XM_015470733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326219.2
CDS	1248..4124
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRP

Target ORF information:

RefSeq Version	XM_015470733.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470733.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470734.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326220.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa234038
Clone ID Related Accession (Same CDS sequence)	XM_015470734.2
Accession Version	XM_015470734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326220.2
CDS	1250..4015
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDG

Target ORF information:

RefSeq Version	XM_015470734.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470734.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470733.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326219.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa89773
Clone ID Related Accession (Same CDS sequence)	XM_015470733.1 , XM_015463116.1
Accession Version	XM_015470733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326219.1
CDS	791..3667
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRP

Target ORF information:

RefSeq Version	XM_015470733.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470733.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463116.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318602.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa89773
Clone ID Related Accession (Same CDS sequence)	XM_015470733.1 , XM_015463116.1
Accession Version	XM_015463116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318602.1
CDS	791..3667
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRP

Target ORF information:

RefSeq Version	XM_015463116.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463116.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470734.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326220.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa89774
Clone ID Related Accession (Same CDS sequence)	XM_015470734.1 , XM_015463117.1
Accession Version	XM_015470734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326220.1
CDS	820..3585
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDG

Target ORF information:

RefSeq Version	XM_015470734.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470734.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463117.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318603.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa89774
Clone ID Related Accession (Same CDS sequence)	XM_015470734.1 , XM_015463117.1
Accession Version	XM_015463117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318603.1
CDS	820..3585
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDG

Target ORF information:

RefSeq Version	XM_015463117.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463117.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470731.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326217.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa234034
Clone ID Related Accession (Same CDS sequence)	XM_015470731.2
Accession Version	XM_015470731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGCACACACA 
GGGGTTCTGT CCCAGCTTCC TACTCCAGCC CCGAAGAAGC CCCTCCTGGT TACCCACATG 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326217.2
CDS	1248..4616
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCERCLCQAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRAHTGVLSQLPTPAPKKPLLVTHMPY

Target ORF information:

RefSeq Version	XM_015470731.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470731.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGCACACACA 
GGGGTTCTGT CCCAGCTTCC TACTCCAGCC CCGAAGAAGC CCCTCCTGGT TACCCACATG 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470732.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326218.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa234035
Clone ID Related Accession (Same CDS sequence)	XM_015470732.2
Accession Version	XM_015470732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGC 
TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG TCCTGCCCGC TCCAGGTCAC 
GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG TTTCATGGGG AGGAGCACCC 
CGAAGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326218.2
CDS	1246..4557
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCWPGQLCAAALPTPVLPAPGHGAGELLSSLQRLPVSWGGAPRRQ

Target ORF information:

RefSeq Version	XM_015470732.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470732.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGC 
TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG TCCTGCCCGC TCCAGGTCAC 
GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG TTTCATGGGG AGGAGCACCC 
CGAAGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991154.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846922.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa234036
Clone ID Related Accession (Same CDS sequence)	XM_024991154.1
Accession Version	XM_024991154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC CGAAGAAGCC CCTCCTGGTT 
ACCCACATGC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846922.1
CDS	1248..4505
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRAHTGVLSQLPTPAPKKPLLVTHMPY

Target ORF information:

RefSeq Version	XM_024991154.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991154.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC CGAAGAAGCC CCTCCTGGTT 
ACCCACATGC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463112.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318598.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X11, mRNA.

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CloneID	OBa89769
Clone ID Related Accession (Same CDS sequence)	XM_015463112.1 , XM_015470729.1
Accession Version	XM_015463112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGACTGTG 
TGGTGGAGGC TGCGGGCCGG ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG 
CCTGTGTCAC GTGCACCTGC CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG 
CGCTCACCTG CCCTCGCGGC TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT 
GCCAAGCCCC CGCCCCGTCG TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT 
GGGACGTGGA CGCCTGCACC AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC 
AGCGCTGCCC GCCGCTCTCC TGTGGGCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318598.1
CDS	794..5146
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQTVWWRLRAGQRRMERAGGTPATPVSRAPAVGAMWSAAWRSARRSPALAAGRRCGRLAGAVRGAKPPPRRARTRAGRWPPGSAGTWTPAPTAPAWQAPCAARASAARRSPVGP

Target ORF information:

RefSeq Version	XM_015463112.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463112.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGACTGTG 
TGGTGGAGGC TGCGGGCCGG ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG 
CCTGTGTCAC GTGCACCTGC CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG 
CGCTCACCTG CCCTCGCGGC TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT 
GCCAAGCCCC CGCCCCGTCG TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT 
GGGACGTGGA CGCCTGCACC AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC 
AGCGCTGCCC GCCGCTCTCC TGTGGGCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470729.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326215.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa89769
Clone ID Related Accession (Same CDS sequence)	XM_015463112.1 , XM_015470729.1
Accession Version	XM_015470729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGACTGTG 
TGGTGGAGGC TGCGGGCCGG ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG 
CCTGTGTCAC GTGCACCTGC CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG 
CGCTCACCTG CCCTCGCGGC TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT 
GCCAAGCCCC CGCCCCGTCG TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT 
GGGACGTGGA CGCCTGCACC AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC 
AGCGCTGCCC GCCGCTCTCC TGTGGGCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326215.1
CDS	794..5146
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQTVWWRLRAGQRRMERAGGTPATPVSRAPAVGAMWSAAWRSARRSPALAAGRRCGRLAGAVRGAKPPPRRARTRAGRWPPGSAGTWTPAPTAPAWQAPCAARASAARRSPVGP

Target ORF information:

RefSeq Version	XM_015470729.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470729.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGACTGTG 
TGGTGGAGGC TGCGGGCCGG ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG 
CCTGTGTCAC GTGCACCTGC CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG 
CGCTCACCTG CCCTCGCGGC TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT 
GCCAAGCCCC CGCCCCGTCG TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT 
GGGACGTGGA CGCCTGCACC AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC 
AGCGCTGCCC GCCGCTCTCC TGTGGGCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463113.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318599.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa89770
Clone ID Related Accession (Same CDS sequence)	XM_015463113.1 , XM_015470730.1
Accession Version	XM_015463113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTGTGAGCT GGGGGAGGGG GGTGGGGGGT GGAGAACCTT ACAGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318599.1
CDS	784..5037
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRVSWGRGVGGGEPYRQK

Target ORF information:

RefSeq Version	XM_015463113.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463113.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTGTGAGCT GGGGGAGGGG GGTGGGGGGT GGAGAACCTT ACAGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470730.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326216.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X12, mRNA.

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CloneID	OBa89770
Clone ID Related Accession (Same CDS sequence)	XM_015463113.1 , XM_015470730.1
Accession Version	XM_015470730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTGTGAGCT GGGGGAGGGG GGTGGGGGGT GGAGAACCTT ACAGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326216.1
CDS	784..5037
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRVSWGRGVGGGEPYRQK

Target ORF information:

RefSeq Version	XM_015470730.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470730.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTGTGAGCT GGGGGAGGGG GGTGGGGGGT GGAGAACCTT ACAGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463114.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318600.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa89771
Clone ID Related Accession (Same CDS sequence)	XM_015463114.1 , XM_015470731.1
Accession Version	XM_015463114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGCACACACA 
GGGGTTCTGT CCCAGCTTCC TACTCCAGCC CCGAAGAAGC CCCTCCTGGT TACCCACATG 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318600.1
CDS	791..4159
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCERCLCQAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRAHTGVLSQLPTPAPKKPLLVTHMPY

Target ORF information:

RefSeq Version	XM_015463114.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463114.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGCACACACA 
GGGGTTCTGT CCCAGCTTCC TACTCCAGCC CCGAAGAAGC CCCTCCTGGT TACCCACATG 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470731.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326217.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa89771
Clone ID Related Accession (Same CDS sequence)	XM_015463114.1 , XM_015470731.1
Accession Version	XM_015470731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGCACACACA 
GGGGTTCTGT CCCAGCTTCC TACTCCAGCC CCGAAGAAGC CCCTCCTGGT TACCCACATG 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326217.1
CDS	791..4159
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCERCLCQAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRAHTGVLSQLPTPAPKKPLLVTHMPY

Target ORF information:

RefSeq Version	XM_015470731.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470731.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGCACACACA 
GGGGTTCTGT CCCAGCTTCC TACTCCAGCC CCGAAGAAGC CCCTCCTGGT TACCCACATG 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470732.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326218.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X16, mRNA.

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CloneID	OBa89772
Clone ID Related Accession (Same CDS sequence)	XM_015470732.1 , XM_015463115.1
Accession Version	XM_015470732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGC 
TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG TCCTGCCCGC TCCAGGTCAC 
GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG TTTCATGGGG AGGAGCACCC 
CGAAGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326218.1
CDS	787..4098
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCWPGQLCAAALPTPVLPAPGHGAGELLSSLQRLPVSWGGAPRRQ

Target ORF information:

RefSeq Version	XM_015470732.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470732.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGC 
TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG TCCTGCCCGC TCCAGGTCAC 
GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG TTTCATGGGG AGGAGCACCC 
CGAAGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463115.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318601.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa89772
Clone ID Related Accession (Same CDS sequence)	XM_015470732.1 , XM_015463115.1
Accession Version	XM_015463115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGC 
TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG TCCTGCCCGC TCCAGGTCAC 
GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG TTTCATGGGG AGGAGCACCC 
CGAAGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318601.1
CDS	787..4098
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCWPGQLCAAALPTPVLPAPGHGAGELLSSLQRLPVSWGGAPRRQ

Target ORF information:

RefSeq Version	XM_015463115.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463115.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGC 
TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG TCCTGCCCGC TCCAGGTCAC 
GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG TTTCATGGGG AGGAGCACCC 
CGAAGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991149.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846917.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa234027
Clone ID Related Accession (Same CDS sequence)	XM_024991149.1 , XM_015470721.2 , XM_024991148.1 , XM_015470722.2 , XM_015470723.2 , XM_024991150.1
Accession Version	XM_024991149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846917.1
CDS	716..6292
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_024991149.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991149.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470721.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326207.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X1, mRNA.

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CloneID	OBa234027
Clone ID Related Accession (Same CDS sequence)	XM_024991149.1 , XM_015470721.2 , XM_024991148.1 , XM_015470722.2 , XM_015470723.2 , XM_024991150.1
Accession Version	XM_015470721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326207.2
CDS	1245..6821
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470721.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470721.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991148.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846916.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X3, mRNA.

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CloneID	OBa234027
Clone ID Related Accession (Same CDS sequence)	XM_024991149.1 , XM_015470721.2 , XM_024991148.1 , XM_015470722.2 , XM_015470723.2 , XM_024991150.1
Accession Version	XM_024991148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846916.1
CDS	1968..7544
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_024991148.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991148.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470722.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326208.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X2, mRNA.

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CloneID	OBa234027
Clone ID Related Accession (Same CDS sequence)	XM_024991149.1 , XM_015470721.2 , XM_024991148.1 , XM_015470722.2 , XM_015470723.2 , XM_024991150.1
Accession Version	XM_015470722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326208.2
CDS	1110..6686
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470722.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470722.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470723.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326209.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X4, mRNA.

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CloneID	OBa234027
Clone ID Related Accession (Same CDS sequence)	XM_024991149.1 , XM_015470721.2 , XM_024991148.1 , XM_015470722.2 , XM_015470723.2 , XM_024991150.1
Accession Version	XM_015470723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326209.2
CDS	984..6560
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470723.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470723.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991150.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846918.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X6, mRNA.

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CloneID	OBa234027
Clone ID Related Accession (Same CDS sequence)	XM_024991149.1 , XM_015470721.2 , XM_024991148.1 , XM_015470722.2 , XM_015470723.2 , XM_024991150.1
Accession Version	XM_024991150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846918.1
CDS	795..6371
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_024991150.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991150.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCGGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470724.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326210.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X7, mRNA.

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CloneID	OBa234028
Clone ID Related Accession (Same CDS sequence)	XM_015470724.2
Accession Version	XM_015470724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGTG AGCGCTGTCT CTGTCAGGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCTC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCGG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326210.2
CDS	1243..6621
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAVSAVSVRLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470724.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470724.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGTG AGCGCTGTCT CTGTCAGGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCTC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCGG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_010804494.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802796.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X8, mRNA.

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CloneID	OBa234029
Clone ID Related Accession (Same CDS sequence)	XM_010804494.3
Accession Version	XM_010804494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG 
TTTCATGGGG AGGAGCACCC CGAAGGCAGT AGCTGGAAGC CCCCTGACAG CCCCTGCTCC 
TCCTGCATGT GTCATGAGGG TGTCATCACC TGTGCCCGCA TCCAGTGTGT CACCTCCTGT 
GCCCAGCCCC ACCAGGGGCC CAGTGACTGC TGCCCTCGGT GCTCTGACTG TGAGCATGAG 
GGTCGGAAGT ATGAGCCAGG AGAGAGCTTC CAGCCCGGTG CAGACCCCTG CGAAGTCTGC 
GTCTGTGAGC TGCAGCCCGA GGGGACACCC AGCCTGCGCT GTCACCGGCG GCAGTGCCCC 
CACCTAGCGG GCTGCCCCAC CAGCCAGCTC CTGACCCCAG GGCCCCAGCA GTGCTGCCCC 
ACCTGTGCCG AGGCGCTGAG CCACTGCACA GAGCACCTGC TGGGGTCTGA GTTGACCCCA 
CCAGACCCCT GCTACAGCTG CCAGTGCCAG GACCTGACTT GGCTCTGCAT TCACCGGGCC 
TGTCCTGAGC TCAGCTGTCC CCTGCCTGAA CGCTACATAC CCTCTGGGAG CTGCTGCCCC 
ATGTGCCAGG ACTGTGTGGT GGAGGCTGCG GGCCGGACAG CGGCGGATGG AGAGAGCTGG 
AGGGACCCCA GCAACGCCTG TGTCACGTGC ACCTGCCGTC GGGGCCATGT GGAGTGCCGC 
CTGGAGGAGT GCCAGGCGCT CACCTGCCCT CGCGGCTGGG CGAAGGTGCG GGAGGCTGGC 
AGGTGCTGTG AGAGGTGCCA AGCCCCCGCC CCGTCGTGCG CGCACCAGGG CCGGCAGGTG 
GCCTCCGGGG AGCGCTGGGA CGTGGACGCC TGCACCAACT GCTCCTGCAT GGCAGGCACC 
GTGCGCTGCC AGAGCCAGCG CTGCCCGCCG CTCTCCTGTG GGCCCGACGA GGCCCCCGCC 
CTGAGTCCCG GCAGCTGCTG TCCTCGCTGC CTGCCCCGGC CCGCTTCCTG CATGGCCTTC 
GGAGACCCTC ATTACCGCAC CTTCGACGGC CGCCTGCTGC ACTTCCAGGG CAGCTGTAGC 
TACGTGCTGG CCAAGGACTG CCGTGGCGGG GACTTCAGCG TGCACGTGAC CAACGATGAC 
CGGGGCCGGA GCGGCGTGTC CTGGACCCAG GAAGTGGCTG TGCTGCTGGG AGACGTGGCC 
GTGCGGCTGC TGCAGGACAG GGCGGTCACG GTGGACGGGC GCCCTGTGGC CTTGCCCTTC 
CTGCAGGAAC CGCTGTTCTA TGTAGAGCTT CGGGGACGCA CGGTGGTGCT GCACTCCCAG 
CCAGGGCTCC AGGTGCTGTG GGATGGGCGG TCCCAGGTGG AGGTGAGAGT GCCTGGCTCC 
TACCGGGGCC ACATGTGTGG GCTCTGCGGC AACTTCAATG GCTTTGCCCA GGATGACCTG 
CGGGACCCTG AGGAACTGCT CCGGTCCACT GAGGCTGCAT TTGGGAATAG CTGGCAGGTC 
CCAGAGGGCC CAGGACCTGG TCGGCCCTGT TCCAAGGGCC GTGAGGTGGA TCCATGCCAG 
GCAGCAGGGT ACCGTGCCAG GCGTGAGGCC AACGCCCGGT GTGCGGTGCT GAAGTCCGCC 
CCGTTCAGCC GCTGCCATGC TGTGGTGCCA CCAGACCCCT TCTTTGCCGC CTGCGTGTAT 
GACCTCTGTG CTTGCGGGCC CGGTTCCTCC TCTGACGCCT GCCTCTGTGA CGCCCTGGAA 
GCCTATGCTA GCCACTGTCG CCAGGCAGGG GTGACACCAG CCTGGCGGGG CCCCACACTC 
TGCGTGGTGG GCTGTCCCCT GGACCGCGGC TTCGTGTTTG ATGAGTGTGG CCCACCCTGT 
CCCCGCACTT GCTTCAACAA GCACGTCCCC CTGGGGGAGC TGGCAGCCCA CTGCGTGCGG 
CCCTGCATCC CCGGCTGCCA GTGCCCAGCG GGCCTGGTGG AGCACGAGGG CCACTGTATC 
TCACCCGAGG CCTGCCCCTC TGTCCTGCTC ACTGGGGACC AGCTGCCCAG CACTCCGCGC 
AACCCCAGCC AGGAGCTTCA GGGGCCAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802796.2
CDS	1241..6553
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCERCLCQAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_010804494.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804494.3

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG 
TTTCATGGGG AGGAGCACCC CGAAGGCAGT AGCTGGAAGC CCCCTGACAG CCCCTGCTCC 
TCCTGCATGT GTCATGAGGG TGTCATCACC TGTGCCCGCA TCCAGTGTGT CACCTCCTGT 
GCCCAGCCCC ACCAGGGGCC CAGTGACTGC TGCCCTCGGT GCTCTGACTG TGAGCATGAG 
GGTCGGAAGT ATGAGCCAGG AGAGAGCTTC CAGCCCGGTG CAGACCCCTG CGAAGTCTGC 
GTCTGTGAGC TGCAGCCCGA GGGGACACCC AGCCTGCGCT GTCACCGGCG GCAGTGCCCC 
CACCTAGCGG GCTGCCCCAC CAGCCAGCTC CTGACCCCAG GGCCCCAGCA GTGCTGCCCC 
ACCTGTGCCG AGGCGCTGAG CCACTGCACA GAGCACCTGC TGGGGTCTGA GTTGACCCCA 
CCAGACCCCT GCTACAGCTG CCAGTGCCAG GACCTGACTT GGCTCTGCAT TCACCGGGCC 
TGTCCTGAGC TCAGCTGTCC CCTGCCTGAA CGCTACATAC CCTCTGGGAG CTGCTGCCCC 
ATGTGCCAGG ACTGTGTGGT GGAGGCTGCG GGCCGGACAG CGGCGGATGG AGAGAGCTGG 
AGGGACCCCA GCAACGCCTG TGTCACGTGC ACCTGCCGTC GGGGCCATGT GGAGTGCCGC 
CTGGAGGAGT GCCAGGCGCT CACCTGCCCT CGCGGCTGGG CGAAGGTGCG GGAGGCTGGC 
AGGTGCTGTG AGAGGTGCCA AGCCCCCGCC CCGTCGTGCG CGCACCAGGG CCGGCAGGTG 
GCCTCCGGGG AGCGCTGGGA CGTGGACGCC TGCACCAACT GCTCCTGCAT GGCAGGCACC 
GTGCGCTGCC AGAGCCAGCG CTGCCCGCCG CTCTCCTGTG GGCCCGACGA GGCCCCCGCC 
CTGAGTCCCG GCAGCTGCTG TCCTCGCTGC CTGCCCCGGC CCGCTTCCTG CATGGCCTTC 
GGAGACCCTC ATTACCGCAC CTTCGACGGC CGCCTGCTGC ACTTCCAGGG CAGCTGTAGC 
TACGTGCTGG CCAAGGACTG CCGTGGCGGG GACTTCAGCG TGCACGTGAC CAACGATGAC 
CGGGGCCGGA GCGGCGTGTC CTGGACCCAG GAAGTGGCTG TGCTGCTGGG AGACGTGGCC 
GTGCGGCTGC TGCAGGACAG GGCGGTCACG GTGGACGGGC GCCCTGTGGC CTTGCCCTTC 
CTGCAGGAAC CGCTGTTCTA TGTAGAGCTT CGGGGACGCA CGGTGGTGCT GCACTCCCAG 
CCAGGGCTCC AGGTGCTGTG GGATGGGCGG TCCCAGGTGG AGGTGAGAGT GCCTGGCTCC 
TACCGGGGCC ACATGTGTGG GCTCTGCGGC AACTTCAATG GCTTTGCCCA GGATGACCTG 
CGGGACCCTG AGGAACTGCT CCGGTCCACT GAGGCTGCAT TTGGGAATAG CTGGCAGGTC 
CCAGAGGGCC CAGGACCTGG TCGGCCCTGT TCCAAGGGCC GTGAGGTGGA TCCATGCCAG 
GCAGCAGGGT ACCGTGCCAG GCGTGAGGCC AACGCCCGGT GTGCGGTGCT GAAGTCCGCC 
CCGTTCAGCC GCTGCCATGC TGTGGTGCCA CCAGACCCCT TCTTTGCCGC CTGCGTGTAT 
GACCTCTGTG CTTGCGGGCC CGGTTCCTCC TCTGACGCCT GCCTCTGTGA CGCCCTGGAA 
GCCTATGCTA GCCACTGTCG CCAGGCAGGG GTGACACCAG CCTGGCGGGG CCCCACACTC 
TGCGTGGTGG GCTGTCCCCT GGACCGCGGC TTCGTGTTTG ATGAGTGTGG CCCACCCTGT 
CCCCGCACTT GCTTCAACAA GCACGTCCCC CTGGGGGAGC TGGCAGCCCA CTGCGTGCGG 
CCCTGCATCC CCGGCTGCCA GTGCCCAGCG GGCCTGGTGG AGCACGAGGG CCACTGTATC 
TCACCCGAGG CCTGCCCCTC TGTCCTGCTC ACTGGGGACC AGCTGCCCAG CACTCCGCGC 
AACCCCAGCC AGGAGCTTCA GGGGCCAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470725.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326211.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X9, mRNA.

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CloneID	OBa234030
Clone ID Related Accession (Same CDS sequence)	XM_015470725.2
Accession Version	XM_015470725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GGCTGCCTGT TTCATGGGGA GGAGCACCCC GAAGGCAGTA GCTGGAAGCC CCCTGACAGC 
CCCTGCTCCT CCTGCATGTG TCATGAGGGT GTCATCACCT GTGCCCGCAT CCAGTGTGTC 
ACCTCCTGTG CCCAGCCCCA CCAGGGGCCC AGTGACTGCT GCCCTCGGTG CTCTGACTGT 
GAGCATGAGG GTCGGAAGTA TGAGCCAGGA GAGAGCTTCC AGCCCGGTGC AGACCCCTGC 
GAAGTCTGCG TCTGTGAGCT GCAGCCCGAG GGGACACCCA GCCTGCGCTG TCACCGGCGG 
CAGTGCCCCC ACCTAGCGGG CTGCCCCACC AGCCAGCTCC TGACCCCAGG GCCCCAGCAG 
TGCTGCCCCA CCTGTGCCGA GGCGCTGAGC CACTGCACAG AGCACCTGCT GGGGTCTGAG 
TTGACCCCAC CAGACCCCTG CTACAGCTGC CAGTGCCAGG ACCTGACTTG GCTCTGCATT 
CACCGGGCCT GTCCTGAGCT CAGCTGTCCC CTGCCTGAAC GCTACATACC CTCTGGGAGC 
TGCTGCCCCA TGTGCCAGGA CTGTGTGGTG GAGGCTGCGG GCCGGACAGC GGCGGATGGA 
GAGAGCTGGA GGGACCCCAG CAACGCCTGT GTCACGTGCA CCTGCCGTCG GGGCCATGTG 
GAGTGCCGCC TGGAGGAGTG CCAGGCGCTC ACCTGCCCTC GCGGCTGGGC GAAGGTGCGG 
GAGGCTGGCA GGTGCTGTGA GAGGTGCCAA GCCCCCGCCC CGTCGTGCGC GCACCAGGGC 
CGGCAGGTGG CCTCCGGGGA GCGCTGGGAC GTGGACGCCT GCACCAACTG CTCCTGCATG 
GCAGGCACCG TGCGCTGCCA GAGCCAGCGC TGCCCGCCGC TCTCCTGTGG GCCCGACGAG 
GCCCCCGCCC TGAGTCCCGG CAGCTGCTGT CCTCGCTGCC TGCCCCGGCC CGCTTCCTGC 
ATGGCCTTCG GAGACCCTCA TTACCGCACC TTCGACGGCC GCCTGCTGCA CTTCCAGGGC 
AGCTGTAGCT ACGTGCTGGC CAAGGACTGC CGTGGCGGGG ACTTCAGCGT GCACGTGACC 
AACGATGACC GGGGCCGGAG CGGCGTGTCC TGGACCCAGG AAGTGGCTGT GCTGCTGGGA 
GACGTGGCCG TGCGGCTGCT GCAGGACAGG GCGGTCACGG TGGACGGGCG CCCTGTGGCC 
TTGCCCTTCC TGCAGGAACC GCTGTTCTAT GTAGAGCTTC GGGGACGCAC GGTGGTGCTG 
CACTCCCAGC CAGGGCTCCA GGTGCTGTGG GATGGGCGGT CCCAGGTGGA GGTGAGAGTG 
CCTGGCTCCT ACCGGGGCCA CATGTGTGGG CTCTGCGGCA ACTTCAATGG CTTTGCCCAG 
GATGACCTGC GGGACCCTGA GGAACTGCTC CGGTCCACTG AGGCTGCATT TGGGAATAGC 
TGGCAGGTCC CAGAGGGCCC AGGACCTGGT CGGCCCTGTT CCAAGGGCCG TGAGGTGGAT 
CCATGCCAGG CAGCAGGGTA CCGTGCCAGG CGTGAGGCCA ACGCCCGGTG TGCGGTGCTG 
AAGTCCGCCC CGTTCAGCCG CTGCCATGCT GTGGTGCCAC CAGACCCCTT CTTTGCCGCC 
TGCGTGTATG ACCTCTGTGC TTGCGGGCCC GGTTCCTCCT CTGACGCCTG CCTCTGTGAC 
GCCCTGGAAG CCTATGCTAG CCACTGTCGC CAGGCAGGGG TGACACCAGC CTGGCGGGGC 
CCCACACTCT GCGTGGTGGG CTGTCCCCTG GACCGCGGCT TCGTGTTTGA TGAGTGTGGC 
CCACCCTGTC CCCGCACTTG CTTCAACAAG CACGTCCCCC TGGGGGAGCT GGCAGCCCAC 
TGCGTGCGGC CCTGCATCCC CGGCTGCCAG TGCCCAGCGG GCCTGGTGGA GCACGAGGGC 
CACTGTATCT CACCCGAGGC CTGCCCCTCT GTCCTGCTCA CTGGGGACCA GCTGCCCAGC 
ACTCCGCGCA ACCCCAGCCA GGAGCTTCAG GGGCCAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326211.2
CDS	1241..6442
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470725.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470725.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GGCTGCCTGT TTCATGGGGA GGAGCACCCC GAAGGCAGTA GCTGGAAGCC CCCTGACAGC 
CCCTGCTCCT CCTGCATGTG TCATGAGGGT GTCATCACCT GTGCCCGCAT CCAGTGTGTC 
ACCTCCTGTG CCCAGCCCCA CCAGGGGCCC AGTGACTGCT GCCCTCGGTG CTCTGACTGT 
GAGCATGAGG GTCGGAAGTA TGAGCCAGGA GAGAGCTTCC AGCCCGGTGC AGACCCCTGC 
GAAGTCTGCG TCTGTGAGCT GCAGCCCGAG GGGACACCCA GCCTGCGCTG TCACCGGCGG 
CAGTGCCCCC ACCTAGCGGG CTGCCCCACC AGCCAGCTCC TGACCCCAGG GCCCCAGCAG 
TGCTGCCCCA CCTGTGCCGA GGCGCTGAGC CACTGCACAG AGCACCTGCT GGGGTCTGAG 
TTGACCCCAC CAGACCCCTG CTACAGCTGC CAGTGCCAGG ACCTGACTTG GCTCTGCATT 
CACCGGGCCT GTCCTGAGCT CAGCTGTCCC CTGCCTGAAC GCTACATACC CTCTGGGAGC 
TGCTGCCCCA TGTGCCAGGA CTGTGTGGTG GAGGCTGCGG GCCGGACAGC GGCGGATGGA 
GAGAGCTGGA GGGACCCCAG CAACGCCTGT GTCACGTGCA CCTGCCGTCG GGGCCATGTG 
GAGTGCCGCC TGGAGGAGTG CCAGGCGCTC ACCTGCCCTC GCGGCTGGGC GAAGGTGCGG 
GAGGCTGGCA GGTGCTGTGA GAGGTGCCAA GCCCCCGCCC CGTCGTGCGC GCACCAGGGC 
CGGCAGGTGG CCTCCGGGGA GCGCTGGGAC GTGGACGCCT GCACCAACTG CTCCTGCATG 
GCAGGCACCG TGCGCTGCCA GAGCCAGCGC TGCCCGCCGC TCTCCTGTGG GCCCGACGAG 
GCCCCCGCCC TGAGTCCCGG CAGCTGCTGT CCTCGCTGCC TGCCCCGGCC CGCTTCCTGC 
ATGGCCTTCG GAGACCCTCA TTACCGCACC TTCGACGGCC GCCTGCTGCA CTTCCAGGGC 
AGCTGTAGCT ACGTGCTGGC CAAGGACTGC CGTGGCGGGG ACTTCAGCGT GCACGTGACC 
AACGATGACC GGGGCCGGAG CGGCGTGTCC TGGACCCAGG AAGTGGCTGT GCTGCTGGGA 
GACGTGGCCG TGCGGCTGCT GCAGGACAGG GCGGTCACGG TGGACGGGCG CCCTGTGGCC 
TTGCCCTTCC TGCAGGAACC GCTGTTCTAT GTAGAGCTTC GGGGACGCAC GGTGGTGCTG 
CACTCCCAGC CAGGGCTCCA GGTGCTGTGG GATGGGCGGT CCCAGGTGGA GGTGAGAGTG 
CCTGGCTCCT ACCGGGGCCA CATGTGTGGG CTCTGCGGCA ACTTCAATGG CTTTGCCCAG 
GATGACCTGC GGGACCCTGA GGAACTGCTC CGGTCCACTG AGGCTGCATT TGGGAATAGC 
TGGCAGGTCC CAGAGGGCCC AGGACCTGGT CGGCCCTGTT CCAAGGGCCG TGAGGTGGAT 
CCATGCCAGG CAGCAGGGTA CCGTGCCAGG CGTGAGGCCA ACGCCCGGTG TGCGGTGCTG 
AAGTCCGCCC CGTTCAGCCG CTGCCATGCT GTGGTGCCAC CAGACCCCTT CTTTGCCGCC 
TGCGTGTATG ACCTCTGTGC TTGCGGGCCC GGTTCCTCCT CTGACGCCTG CCTCTGTGAC 
GCCCTGGAAG CCTATGCTAG CCACTGTCGC CAGGCAGGGG TGACACCAGC CTGGCGGGGC 
CCCACACTCT GCGTGGTGGG CTGTCCCCTG GACCGCGGCT TCGTGTTTGA TGAGTGTGGC 
CCACCCTGTC CCCGCACTTG CTTCAACAAG CACGTCCCCC TGGGGGAGCT GGCAGCCCAC 
TGCGTGCGGC CCTGCATCCC CGGCTGCCAG TGCCCAGCGG GCCTGGTGGA GCACGAGGGC 
CACTGTATCT CACCCGAGGC CTGCCCCTCT GTCCTGCTCA CTGGGGACCA GCTGCCCAGC 
ACTCCGCGCA ACCCCAGCCA GGAGCTTCAG GGGCCAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991151.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846919.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X10, mRNA.

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CloneID	OBa234031
Clone ID Related Accession (Same CDS sequence)	XM_024991151.1 , XM_024991152.1
Accession Version	XM_024991151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGGAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCTCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCGGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846919.1
CDS	237..5201
Translation	MVAPPGGAAGKAGGRGHGAQRTGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_024991151.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991151.1

ORF Insert Sequence:

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGGAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCTCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCGGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846920.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X11, mRNA.

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CloneID	OBa234031
Clone ID Related Accession (Same CDS sequence)	XM_024991151.1 , XM_024991152.1
Accession Version	XM_024991152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGGAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCTCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCGGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846920.1
CDS	874..5838
Translation	MVAPPGGAAGKAGGRGHGAQRTGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_024991152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991152.1

ORF Insert Sequence:

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGGAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCTCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCGGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470728.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326214.2 Bos taurus kielin/chordin-like protein (KCP), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa234032
Clone ID Related Accession (Same CDS sequence)	XM_015470728.2
Accession Version	XM_015470728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGGGG CATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326214.2
CDS	1251..6098
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQGAC

Target ORF information:

RefSeq Version	XM_015470728.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470728.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGACGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGGAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCTCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGGGG CATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_024991153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846921.1 Bos taurus kielin/chordin-like protein (KCP), transcript variant X13, mRNA.

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CloneID	OBa234033
Clone ID Related Accession (Same CDS sequence)	XM_024991153.1
Accession Version	XM_024991153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAT GTGGCAATTG GAGCGCTGTG TTATCTGCAC CTGCCAGGCA GGGACAGTGC 
GGTGCCAGGG GCCCTCATGC TCAGAGCTCA ACTGCCTGGA GAGCTACACC CCACCTGGGG 
AGTGCTGCCC CGTCTGCCGG CCAGGCTGTG AGTATGAGGG GCAGCTTTAC GAGGAAGGGG 
CCAACTTCTG GTCCAGCTCC AACCCTTGTC TCCAGTGCTC CTGCCTGCCT GCTCTCTCTG 
GGAGCCAGAG CTGGAGGCAG CACAGCTGTG GGTGTGTTAC AGAGGAGCCT GGTTCGCTGC 
GTGCCCACGA AGTGCCCGCC CATCCCCTGC CCCAAGCCAG TCCTGAGGCC TGGGCACTGC 
TGCCCGACCT GCCAAGCTCA AGGCTGCACA GAAAATGGTT CTCACTGGGA GCATGGCCAA 
GAGTGGACCA CCCCTGGGGA CCCCTGCCGG ATCTGCCAGT GCCTGGAAGG CCACATCCTG 
TGCCGCCAGC GGGAATGTGC CAGTCTGTGC CCATACCCTG CCCGGCCCCT CCCGGGCACC 
TGCTGCCCGG TGTGTGATGG CTGTTTCCTA AATGGGCGGG ACTACCGCAG CGGGGAGCCC 
GTGGGCTCTG GGGACCCCTG CTCGCGCTGC CGTTGTGTCA ATGGGAGTGT CCAGTGTGAG 
CCTCCACCCT GCCCACCCAC GCCCTGCAGA CACCCAGGCA GGACCCCGGG GGAGTGCTGC 
CCTGTCTGTG ACAGCTGTGA GTACCAGGGA CACAAGTATC AGAACCAGGA GACCTTCCAG 
CTCCAAGAGA GTGGCCGCTG TGCCCACTGC TCCTGCCAGG CTGGTGAAGT CTCCTGCGAG 
GAGCGTGAGT GCCCAGGCAT GCCCTGCACC CTGCCTGACT CTGGCCCCGA GCCCTGCTCA 
GTCTGCGTGC TTGATGGAGA GGAGTTTGCT GAGGGTGTCC AGTGGGAGCC TGATGGCCAG 
CCCTGCACGA CCTGCTCCTG TCAAGCTGGG GTACCTGTGT GTGGGACTTT GCTCTGCTCC 
CCAGCCCCCT GCCAGCACCC CACCCAGCCC CCGGGTGCCT GCTGCCCCAG CTGTGAGAAT 
TGCACTTACC ATGGCCAAGT GTATGCCAAC GGGCAGAACT TCACAGACGC AGACCCTTGC 
CACACCTGCC ACTGCGAGGA TGGGACCGTG ACGTGCTCCT TGGTCAACTG CCCTCCCACA 
ACCTGTGCCA GGCCTCAGAG GGGACCCAGC CAATGCTGCC CCAGGTGCCC AGACTGCGTC 
CTGGAGAAAC AGGTGTTTTT GGACGGTGAA CGCTTCTCGC ACCCCCGAGA TCCCTGCCAG 
GAATGCCAGT GCCGGGAAGG CCATGCCCAC TGCCAACCCC GGCTCTGCCC CCGGACCTCC 
TGTGCCCACC CGCTGCCTGG TGTCTGTTGC CAGAACAACT GCAATGGTTG TGCCTTTGGT 
GGGAAAGAGT ACCCCAACGG GGCCGACTTC CCCCACCCCT CCGATCCCTG CCGTCTGTGT 
CACTGTCTGG GAGGCACTGT GCAGTGCCTG GCGCGCCGCT GCCCACCCTT GCCCTGCCCA 
GAGCCGGTCC TGCTGCCCAG ACAGTGCTGC CCACGGTGCC CAGCTGCCCC CTCGGGCTGC 
CCCCGGCCAG GGGGCTTGGT CCCTGCCCAC CACCAGGAGT ACTTCTCCCC GCCCGACGAC 
CCCTGCCGCC GCTGCCTCTG CCTGGACGGC TCGGTGTCCT GCCGGAGACT GCCCTGCCCT 
CCCGTGCCCT GCACCCACCC GCACCAGGGG CCCTGCTGCC CCTCCTGTGA CGGCTGCCTG 
TACCAGGGGA AGGAGTTCAC CAGCGGGGAG CGCTTCCCTT CGCCCACTGC CAGATGTCAC 
ATCTGCCTCT GCTGGGAGGG CAGCATCAGC TGTGAGCCCA GGGCCTGTGC ACCTGCCCAG 
TGCCCCTTCC CTGCCCAGGG TGACTGCTGC CCTGCCTGTG ATGGCTGTGA GTATCTGGGG 
GAGTCCTACC TGAGTGGCCA GGAGTTCCCA GATCCCCGCG AGCCCTGCAA CCTGTGTACC 
TGCCTCGGAG GCTTCGTGAC CTGCGGCCGC CGGCCCTGTG AGCCCCTGGG CTGCAGCCAC 
CCACTCACCC GAGCTGGGCA CTGCTGCCCG ACCTGCCAGG GATGCCTCTA TCATGGCGTC 
ACTGCTGCCC CTGGAGAGAC CCTTCCTGAC CCACTAGACC CCGCCTGCTC GCTCTGCACC 
TGCCAGGAAG GCTCCATGCG CTGCCAGAAG AAGCCGTGTC CTCCAGCTCT CTGCCCTCGC 
CCCTCTTCAG GACCCTGCTT CTGCCCCGTG TGCCACAGTG AGCACACACG CCTGCCCCCA 
CGCGCTTCTC TGCCCCTCCT CCCTCCCTCT CTGCCAGGCC TGAGGTGGGA CCGGGCAAGG 
TGGCTCGACC CTGCACCCTC ACCCAGGACA CGTGTCGCCC ACAGGCTGCC TCTCTCAGGG 
CCAGGAGCAC CAGGACGGGG AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCTC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCGG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846921.1
CDS	163..4941
Translation	MGKCGNWSAVLSAPARQGQCGARGPHAQSSTAWRATPHLGSAAPSAGQAVSMRGSFTRKGPTSGPAPTLVSSAPACLLSLGARAGGSTAVGVLQRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDGEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPSGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPGCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_024991153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (KCP), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991153.1

ORF Insert Sequence:

ATGGGGAAAT GTGGCAATTG GAGCGCTGTG TTATCTGCAC CTGCCAGGCA GGGACAGTGC 
GGTGCCAGGG GCCCTCATGC TCAGAGCTCA ACTGCCTGGA GAGCTACACC CCACCTGGGG 
AGTGCTGCCC CGTCTGCCGG CCAGGCTGTG AGTATGAGGG GCAGCTTTAC GAGGAAGGGG 
CCAACTTCTG GTCCAGCTCC AACCCTTGTC TCCAGTGCTC CTGCCTGCCT GCTCTCTCTG 
GGAGCCAGAG CTGGAGGCAG CACAGCTGTG GGTGTGTTAC AGAGGAGCCT GGTTCGCTGC 
GTGCCCACGA AGTGCCCGCC CATCCCCTGC CCCAAGCCAG TCCTGAGGCC TGGGCACTGC 
TGCCCGACCT GCCAAGCTCA AGGCTGCACA GAAAATGGTT CTCACTGGGA GCATGGCCAA 
GAGTGGACCA CCCCTGGGGA CCCCTGCCGG ATCTGCCAGT GCCTGGAAGG CCACATCCTG 
TGCCGCCAGC GGGAATGTGC CAGTCTGTGC CCATACCCTG CCCGGCCCCT CCCGGGCACC 
TGCTGCCCGG TGTGTGATGG CTGTTTCCTA AATGGGCGGG ACTACCGCAG CGGGGAGCCC 
GTGGGCTCTG GGGACCCCTG CTCGCGCTGC CGTTGTGTCA ATGGGAGTGT CCAGTGTGAG 
CCTCCACCCT GCCCACCCAC GCCCTGCAGA CACCCAGGCA GGACCCCGGG GGAGTGCTGC 
CCTGTCTGTG ACAGCTGTGA GTACCAGGGA CACAAGTATC AGAACCAGGA GACCTTCCAG 
CTCCAAGAGA GTGGCCGCTG TGCCCACTGC TCCTGCCAGG CTGGTGAAGT CTCCTGCGAG 
GAGCGTGAGT GCCCAGGCAT GCCCTGCACC CTGCCTGACT CTGGCCCCGA GCCCTGCTCA 
GTCTGCGTGC TTGATGGAGA GGAGTTTGCT GAGGGTGTCC AGTGGGAGCC TGATGGCCAG 
CCCTGCACGA CCTGCTCCTG TCAAGCTGGG GTACCTGTGT GTGGGACTTT GCTCTGCTCC 
CCAGCCCCCT GCCAGCACCC CACCCAGCCC CCGGGTGCCT GCTGCCCCAG CTGTGAGAAT 
TGCACTTACC ATGGCCAAGT GTATGCCAAC GGGCAGAACT TCACAGACGC AGACCCTTGC 
CACACCTGCC ACTGCGAGGA TGGGACCGTG ACGTGCTCCT TGGTCAACTG CCCTCCCACA 
ACCTGTGCCA GGCCTCAGAG GGGACCCAGC CAATGCTGCC CCAGGTGCCC AGACTGCGTC 
CTGGAGAAAC AGGTGTTTTT GGACGGTGAA CGCTTCTCGC ACCCCCGAGA TCCCTGCCAG 
GAATGCCAGT GCCGGGAAGG CCATGCCCAC TGCCAACCCC GGCTCTGCCC CCGGACCTCC 
TGTGCCCACC CGCTGCCTGG TGTCTGTTGC CAGAACAACT GCAATGGTTG TGCCTTTGGT 
GGGAAAGAGT ACCCCAACGG GGCCGACTTC CCCCACCCCT CCGATCCCTG CCGTCTGTGT 
CACTGTCTGG GAGGCACTGT GCAGTGCCTG GCGCGCCGCT GCCCACCCTT GCCCTGCCCA 
GAGCCGGTCC TGCTGCCCAG ACAGTGCTGC CCACGGTGCC CAGCTGCCCC CTCGGGCTGC 
CCCCGGCCAG GGGGCTTGGT CCCTGCCCAC CACCAGGAGT ACTTCTCCCC GCCCGACGAC 
CCCTGCCGCC GCTGCCTCTG CCTGGACGGC TCGGTGTCCT GCCGGAGACT GCCCTGCCCT 
CCCGTGCCCT GCACCCACCC GCACCAGGGG CCCTGCTGCC CCTCCTGTGA CGGCTGCCTG 
TACCAGGGGA AGGAGTTCAC CAGCGGGGAG CGCTTCCCTT CGCCCACTGC CAGATGTCAC 
ATCTGCCTCT GCTGGGAGGG CAGCATCAGC TGTGAGCCCA GGGCCTGTGC ACCTGCCCAG 
TGCCCCTTCC CTGCCCAGGG TGACTGCTGC CCTGCCTGTG ATGGCTGTGA GTATCTGGGG 
GAGTCCTACC TGAGTGGCCA GGAGTTCCCA GATCCCCGCG AGCCCTGCAA CCTGTGTACC 
TGCCTCGGAG GCTTCGTGAC CTGCGGCCGC CGGCCCTGTG AGCCCCTGGG CTGCAGCCAC 
CCACTCACCC GAGCTGGGCA CTGCTGCCCG ACCTGCCAGG GATGCCTCTA TCATGGCGTC 
ACTGCTGCCC CTGGAGAGAC CCTTCCTGAC CCACTAGACC CCGCCTGCTC GCTCTGCACC 
TGCCAGGAAG GCTCCATGCG CTGCCAGAAG AAGCCGTGTC CTCCAGCTCT CTGCCCTCGC 
CCCTCTTCAG GACCCTGCTT CTGCCCCGTG TGCCACAGTG AGCACACACG CCTGCCCCCA 
CGCGCTTCTC TGCCCCTCCT CCCTCCCTCT CTGCCAGGCC TGAGGTGGGA CCGGGCAAGG 
TGGCTCGACC CTGCACCCTC ACCCAGGACA CGTGTCGCCC ACAGGCTGCC TCTCTCAGGG 
CCAGGAGCAC CAGGACGGGG AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCTC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCGG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470721.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326207.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X1, mRNA.

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CloneID	OBa89763
Clone ID Related Accession (Same CDS sequence)	XM_015470721.1 , XM_015470723.1 , XM_015463104.1 , XM_015463105.1 , XM_015463103.1 , XM_015470722.1
Accession Version	XM_015470721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326207.1
CDS	771..6347
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470721.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470721.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470723.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326209.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X3, mRNA.

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CloneID	OBa89763
Clone ID Related Accession (Same CDS sequence)	XM_015470721.1 , XM_015470723.1 , XM_015463104.1 , XM_015463105.1 , XM_015463103.1 , XM_015470722.1
Accession Version	XM_015470723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326209.1
CDS	510..6086
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470723.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470723.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463104.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318590.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X2, mRNA.

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CloneID	OBa89763
Clone ID Related Accession (Same CDS sequence)	XM_015470721.1 , XM_015470723.1 , XM_015463104.1 , XM_015463105.1 , XM_015463103.1 , XM_015470722.1
Accession Version	XM_015463104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318590.1
CDS	636..6212
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463104.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463104.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463105.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318591.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X3, mRNA.

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CloneID	OBa89763
Clone ID Related Accession (Same CDS sequence)	XM_015470721.1 , XM_015470723.1 , XM_015463104.1 , XM_015463105.1 , XM_015463103.1 , XM_015470722.1
Accession Version	XM_015463105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318591.1
CDS	510..6086
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463105.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463105.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463103.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318589.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X1, mRNA.

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CloneID	OBa89763
Clone ID Related Accession (Same CDS sequence)	XM_015470721.1 , XM_015470723.1 , XM_015463104.1 , XM_015463105.1 , XM_015463103.1 , XM_015470722.1
Accession Version	XM_015463103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318589.1
CDS	771..6347
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463103.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463103.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470722.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326208.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X2, mRNA.

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CloneID	OBa89763
Clone ID Related Accession (Same CDS sequence)	XM_015470721.1 , XM_015470723.1 , XM_015463104.1 , XM_015463105.1 , XM_015463103.1 , XM_015470722.1
Accession Version	XM_015470722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326208.1
CDS	636..6212
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470722.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470722.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGTCCCAGAG GGCCCAGGAC CTGGTCGGCC CTGTTCCAAG 
GGCCGTGAGG TGGATCCATG CCAGGCAGCA GGGTACCGTG CCAGGCGTGA GGCCAACGCC 
CGGTGTGCGG TGCTGAAGTC CGCCCCGTTC AGCCGCTGCC ATGCTGTGGT GCCACCAGAC 
CCCTTCTTTG CCGCCTGCGT GTATGACCTC TGTGCTTGCG GGCCCGGTTC CTCCTCTGAC 
GCCTGCCTCT GTGACGCCCT GGAAGCCTAT GCTAGCCACT GTCGCCAGGC AGGGGTGACA 
CCAGCCTGGC GGGGCCCCAC ACTCTGCGTG GTGGGCTGTC CCCTGGACCG CGGCTTCGTG 
TTTGATGAGT GTGGCCCACC CTGTCCCCGC ACTTGCTTCA ACAAGCACGT CCCCCTGGGG 
GAGCTGGCAG CCCACTGCGT GCGGCCCTGC ATCCCCAGCT GCCAGTGCCC AGCGGGCCTG 
GTGGAGCACG AGGGCCACTG TATCTCACCC GAGGCCTGCC CCTCTGTCCT GCTCACTGGG 
GACCAGCTGC CCAGCACTCC GCGCAACCCC AGCCAGGAGC TTCAGGGGCC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470724.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326210.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X4, mRNA.

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CloneID	OBa89764
Clone ID Related Accession (Same CDS sequence)	XM_015470724.1 , XM_015463106.1
Accession Version	XM_015470724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGTG AGCGCTGTCT CTGTCAGGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCCC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCAG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326210.1
CDS	771..6149
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAVSAVSVRLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470724.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470724.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGTG AGCGCTGTCT CTGTCAGGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCCC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCAG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463106.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318592.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X4, mRNA.

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CloneID	OBa89764
Clone ID Related Accession (Same CDS sequence)	XM_015470724.1 , XM_015463106.1
Accession Version	XM_015463106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGTG AGCGCTGTCT CTGTCAGGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCCC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCAG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318592.1
CDS	771..6149
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAVSAVSVRLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463106.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463106.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGTG AGCGCTGTCT CTGTCAGGCT GGCCAGGTCA 
GCTGTGTGCG GCAGCGCTGC CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA 
GCTGCTGTCC TCGCTGCAGA GCACACACAG GGGTTCTGTC CCAGCTTCCT ACTCCAGCCC 
CGAAGAAGCC CCTCCTGGTT ACCCACATGC CCTACTGACT ACGCAGAGTG GGGCAGGGGA 
TCGGGGCAGA CATGCAGAAC CCAGACTCAG AGCTCACCCC TGCCCACCTT GGACACAGGC 
TGCCTGTTTC ATGGGGAGGA GCACCCCGAA GGCAGTAGCT GGAAGCCCCC TGACAGCCCC 
TGCTCCTCCT GCATGTGTCA TGAGGGTGTC ATCACCTGTG CCCGCATCCA GTGTGTCACC 
TCCTGTGCCC AGCCCCACCA GGGGCCCAGT GACTGCTGCC CTCGGTGCTC TGACTGTGAG 
CATGAGGGTC GGAAGTATGA GCCAGGAGAG AGCTTCCAGC CCGGTGCAGA CCCCTGCGAA 
GTCTGCGTCT GTGAGCTGCA GCCCGAGGGG ACACCCAGCC TGCGCTGTCA CCGGCGGCAG 
TGCCCCCACC TAGCGGGCTG CCCCACCAGC CAGCTCCTGA CCCCAGGGCC CCAGCAGTGC 
TGCCCCACCT GTGCCGAGGC GCTGAGCCAC TGCACAGAGC ACCTGCTGGG GTCTGAGTTG 
ACCCCACCAG ACCCCTGCTA CAGCTGCCAG TGCCAGGACC TGACTTGGCT CTGCATTCAC 
CGGGCCTGTC CTGAGCTCAG CTGTCCCCTG CCTGAACGCT ACATACCCCC TGGGAGCTGC 
TGCCCCATGT GCCAGGACTG TGTGGTGGAG GCTGCGGGCC GGACAGCGGC GGATGGAGAG 
AGCTGGAGGG ACCCCAGCAA CGCCTGTGTC ACGTGCACCT GCCGTCGGGG CCATGTGGAG 
TGCCGCCTGG AGGAGTGCCA GGCGCTCACC TGCCCTCGCG GCTGGGCGAA GGTGCGGGAG 
GCTGGCAGGT GCTGTGAGAG GTGCCAAGCC CCCGCCCCGT CGTGCGCGCA CCAGGGCCGG 
CAGGTGGCCT CCGGGGAGCG CTGGGACGTG GACGCCTGCA CCAACTGCTC CTGCATGGCA 
GGCACCGTGC GCTGCCAGAG CCAGCGCTGC CCGCCGCTCT CCTGTGGGCC CGACGAGGCC 
CCCGCCCTGA GTCCCGGCAG CTGCTGTCCT CGCTGCCTGC CCCGGCCCGC TTCCTGCATG 
GCCTTCGGAG ACCCTCATTA CCGCACCTTC GACGGCCGCC TGCTGCACTT CCAGGGCAGC 
TGTAGCTACG TGCTGGCCAA GGACTGCCGT GGCGGGGACT TCAGCGTGCA CGTGACCAAC 
GATGACCGGG GCCGGAGCGG CGTGTCCTGG ACCCAGGAAG TGGCTGTGCT GCTGGGAGAC 
GTGGCCGTGC GGCTGCTGCA GGACAGGGCG GTCACGGTGG ACGGGCGCCC TGTGGCCTTG 
CCCTTCCTGC AGGAACCGCT GTTCTATGTA GAGCTTCGGG GACGCACGGT GGTGCTGCAC 
TCCCAGCCAG GGCTCCAGGT GCTGTGGGAT GGGCGGTCCC AGGTGGAGGT GAGAGTGCCT 
GGCTCCTACC GGGGCCACAT GTGTGGGCTC TGCGGCAACT TCAATGGCTT TGCCCAGGAT 
GACCTGCGGG ACCCTGAGGA ACTGCTCCGG TCCACTGAGG CTGCATTTGG GAATAGCTGG 
CAGGTCCCAG AGGGCCCAGG ACCTGGTCGG CCCTGTTCCA AGGGCCGTGA GGTGGATCCA 
TGCCAGGCAG CAGGGTACCG TGCCAGGCGT GAGGCCAACG CCCGGTGTGC GGTGCTGAAG 
TCCGCCCCGT TCAGCCGCTG CCATGCTGTG GTGCCACCAG ACCCCTTCTT TGCCGCCTGC 
GTGTATGACC TCTGTGCTTG CGGGCCCGGT TCCTCCTCTG ACGCCTGCCT CTGTGACGCC 
CTGGAAGCCT ATGCTAGCCA CTGTCGCCAG GCAGGGGTGA CACCAGCCTG GCGGGGCCCC 
ACACTCTGCG TGGTGGGCTG TCCCCTGGAC CGCGGCTTCG TGTTTGATGA GTGTGGCCCA 
CCCTGTCCCC GCACTTGCTT CAACAAGCAC GTCCCCCTGG GGGAGCTGGC AGCCCACTGC 
GTGCGGCCCT GCATCCCCAG CTGCCAGTGC CCAGCGGGCC TGGTGGAGCA CGAGGGCCAC 
TGTATCTCAC CCGAGGCCTG CCCCTCTGTC CTGCTCACTG GGGACCAGCT GCCCAGCACT 
CCGCGCAACC CCAGCCAGGA GCTTCAGGGG CCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463108.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318594.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X6, mRNA.

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CloneID	OBa89765
Clone ID Related Accession (Same CDS sequence)	XM_015463108.1 , XM_015470725.1
Accession Version	XM_015463108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GGCTGCCTGT TTCATGGGGA GGAGCACCCC GAAGGCAGTA GCTGGAAGCC CCCTGACAGC 
CCCTGCTCCT CCTGCATGTG TCATGAGGGT GTCATCACCT GTGCCCGCAT CCAGTGTGTC 
ACCTCCTGTG CCCAGCCCCA CCAGGGGCCC AGTGACTGCT GCCCTCGGTG CTCTGACTGT 
GAGCATGAGG GTCGGAAGTA TGAGCCAGGA GAGAGCTTCC AGCCCGGTGC AGACCCCTGC 
GAAGTCTGCG TCTGTGAGCT GCAGCCCGAG GGGACACCCA GCCTGCGCTG TCACCGGCGG 
CAGTGCCCCC ACCTAGCGGG CTGCCCCACC AGCCAGCTCC TGACCCCAGG GCCCCAGCAG 
TGCTGCCCCA CCTGTGCCGA GGCGCTGAGC CACTGCACAG AGCACCTGCT GGGGTCTGAG 
TTGACCCCAC CAGACCCCTG CTACAGCTGC CAGTGCCAGG ACCTGACTTG GCTCTGCATT 
CACCGGGCCT GTCCTGAGCT CAGCTGTCCC CTGCCTGAAC GCTACATACC CCCTGGGAGC 
TGCTGCCCCA TGTGCCAGGA CTGTGTGGTG GAGGCTGCGG GCCGGACAGC GGCGGATGGA 
GAGAGCTGGA GGGACCCCAG CAACGCCTGT GTCACGTGCA CCTGCCGTCG GGGCCATGTG 
GAGTGCCGCC TGGAGGAGTG CCAGGCGCTC ACCTGCCCTC GCGGCTGGGC GAAGGTGCGG 
GAGGCTGGCA GGTGCTGTGA GAGGTGCCAA GCCCCCGCCC CGTCGTGCGC GCACCAGGGC 
CGGCAGGTGG CCTCCGGGGA GCGCTGGGAC GTGGACGCCT GCACCAACTG CTCCTGCATG 
GCAGGCACCG TGCGCTGCCA GAGCCAGCGC TGCCCGCCGC TCTCCTGTGG GCCCGACGAG 
GCCCCCGCCC TGAGTCCCGG CAGCTGCTGT CCTCGCTGCC TGCCCCGGCC CGCTTCCTGC 
ATGGCCTTCG GAGACCCTCA TTACCGCACC TTCGACGGCC GCCTGCTGCA CTTCCAGGGC 
AGCTGTAGCT ACGTGCTGGC CAAGGACTGC CGTGGCGGGG ACTTCAGCGT GCACGTGACC 
AACGATGACC GGGGCCGGAG CGGCGTGTCC TGGACCCAGG AAGTGGCTGT GCTGCTGGGA 
GACGTGGCCG TGCGGCTGCT GCAGGACAGG GCGGTCACGG TGGACGGGCG CCCTGTGGCC 
TTGCCCTTCC TGCAGGAACC GCTGTTCTAT GTAGAGCTTC GGGGACGCAC GGTGGTGCTG 
CACTCCCAGC CAGGGCTCCA GGTGCTGTGG GATGGGCGGT CCCAGGTGGA GGTGAGAGTG 
CCTGGCTCCT ACCGGGGCCA CATGTGTGGG CTCTGCGGCA ACTTCAATGG CTTTGCCCAG 
GATGACCTGC GGGACCCTGA GGAACTGCTC CGGTCCACTG AGGCTGCATT TGGGAATAGC 
TGGCAGGTCC CAGAGGGCCC AGGACCTGGT CGGCCCTGTT CCAAGGGCCG TGAGGTGGAT 
CCATGCCAGG CAGCAGGGTA CCGTGCCAGG CGTGAGGCCA ACGCCCGGTG TGCGGTGCTG 
AAGTCCGCCC CGTTCAGCCG CTGCCATGCT GTGGTGCCAC CAGACCCCTT CTTTGCCGCC 
TGCGTGTATG ACCTCTGTGC TTGCGGGCCC GGTTCCTCCT CTGACGCCTG CCTCTGTGAC 
GCCCTGGAAG CCTATGCTAG CCACTGTCGC CAGGCAGGGG TGACACCAGC CTGGCGGGGC 
CCCACACTCT GCGTGGTGGG CTGTCCCCTG GACCGCGGCT TCGTGTTTGA TGAGTGTGGC 
CCACCCTGTC CCCGCACTTG CTTCAACAAG CACGTCCCCC TGGGGGAGCT GGCAGCCCAC 
TGCGTGCGGC CCTGCATCCC CAGCTGCCAG TGCCCAGCGG GCCTGGTGGA GCACGAGGGC 
CACTGTATCT CACCCGAGGC CTGCCCCTCT GTCCTGCTCA CTGGGGACCA GCTGCCCAGC 
ACTCCGCGCA ACCCCAGCCA GGAGCTTCAG GGGCCAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318594.1
CDS	770..5971
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463108.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463108.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GGCTGCCTGT TTCATGGGGA GGAGCACCCC GAAGGCAGTA GCTGGAAGCC CCCTGACAGC 
CCCTGCTCCT CCTGCATGTG TCATGAGGGT GTCATCACCT GTGCCCGCAT CCAGTGTGTC 
ACCTCCTGTG CCCAGCCCCA CCAGGGGCCC AGTGACTGCT GCCCTCGGTG CTCTGACTGT 
GAGCATGAGG GTCGGAAGTA TGAGCCAGGA GAGAGCTTCC AGCCCGGTGC AGACCCCTGC 
GAAGTCTGCG TCTGTGAGCT GCAGCCCGAG GGGACACCCA GCCTGCGCTG TCACCGGCGG 
CAGTGCCCCC ACCTAGCGGG CTGCCCCACC AGCCAGCTCC TGACCCCAGG GCCCCAGCAG 
TGCTGCCCCA CCTGTGCCGA GGCGCTGAGC CACTGCACAG AGCACCTGCT GGGGTCTGAG 
TTGACCCCAC CAGACCCCTG CTACAGCTGC CAGTGCCAGG ACCTGACTTG GCTCTGCATT 
CACCGGGCCT GTCCTGAGCT CAGCTGTCCC CTGCCTGAAC GCTACATACC CCCTGGGAGC 
TGCTGCCCCA TGTGCCAGGA CTGTGTGGTG GAGGCTGCGG GCCGGACAGC GGCGGATGGA 
GAGAGCTGGA GGGACCCCAG CAACGCCTGT GTCACGTGCA CCTGCCGTCG GGGCCATGTG 
GAGTGCCGCC TGGAGGAGTG CCAGGCGCTC ACCTGCCCTC GCGGCTGGGC GAAGGTGCGG 
GAGGCTGGCA GGTGCTGTGA GAGGTGCCAA GCCCCCGCCC CGTCGTGCGC GCACCAGGGC 
CGGCAGGTGG CCTCCGGGGA GCGCTGGGAC GTGGACGCCT GCACCAACTG CTCCTGCATG 
GCAGGCACCG TGCGCTGCCA GAGCCAGCGC TGCCCGCCGC TCTCCTGTGG GCCCGACGAG 
GCCCCCGCCC TGAGTCCCGG CAGCTGCTGT CCTCGCTGCC TGCCCCGGCC CGCTTCCTGC 
ATGGCCTTCG GAGACCCTCA TTACCGCACC TTCGACGGCC GCCTGCTGCA CTTCCAGGGC 
AGCTGTAGCT ACGTGCTGGC CAAGGACTGC CGTGGCGGGG ACTTCAGCGT GCACGTGACC 
AACGATGACC GGGGCCGGAG CGGCGTGTCC TGGACCCAGG AAGTGGCTGT GCTGCTGGGA 
GACGTGGCCG TGCGGCTGCT GCAGGACAGG GCGGTCACGG TGGACGGGCG CCCTGTGGCC 
TTGCCCTTCC TGCAGGAACC GCTGTTCTAT GTAGAGCTTC GGGGACGCAC GGTGGTGCTG 
CACTCCCAGC CAGGGCTCCA GGTGCTGTGG GATGGGCGGT CCCAGGTGGA GGTGAGAGTG 
CCTGGCTCCT ACCGGGGCCA CATGTGTGGG CTCTGCGGCA ACTTCAATGG CTTTGCCCAG 
GATGACCTGC GGGACCCTGA GGAACTGCTC CGGTCCACTG AGGCTGCATT TGGGAATAGC 
TGGCAGGTCC CAGAGGGCCC AGGACCTGGT CGGCCCTGTT CCAAGGGCCG TGAGGTGGAT 
CCATGCCAGG CAGCAGGGTA CCGTGCCAGG CGTGAGGCCA ACGCCCGGTG TGCGGTGCTG 
AAGTCCGCCC CGTTCAGCCG CTGCCATGCT GTGGTGCCAC CAGACCCCTT CTTTGCCGCC 
TGCGTGTATG ACCTCTGTGC TTGCGGGCCC GGTTCCTCCT CTGACGCCTG CCTCTGTGAC 
GCCCTGGAAG CCTATGCTAG CCACTGTCGC CAGGCAGGGG TGACACCAGC CTGGCGGGGC 
CCCACACTCT GCGTGGTGGG CTGTCCCCTG GACCGCGGCT TCGTGTTTGA TGAGTGTGGC 
CCACCCTGTC CCCGCACTTG CTTCAACAAG CACGTCCCCC TGGGGGAGCT GGCAGCCCAC 
TGCGTGCGGC CCTGCATCCC CAGCTGCCAG TGCCCAGCGG GCCTGGTGGA GCACGAGGGC 
CACTGTATCT CACCCGAGGC CTGCCCCTCT GTCCTGCTCA CTGGGGACCA GCTGCCCAGC 
ACTCCGCGCA ACCCCAGCCA GGAGCTTCAG GGGCCAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470725.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326211.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X6, mRNA.

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CloneID	OBa89765
Clone ID Related Accession (Same CDS sequence)	XM_015463108.1 , XM_015470725.1
Accession Version	XM_015470725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GGCTGCCTGT TTCATGGGGA GGAGCACCCC GAAGGCAGTA GCTGGAAGCC CCCTGACAGC 
CCCTGCTCCT CCTGCATGTG TCATGAGGGT GTCATCACCT GTGCCCGCAT CCAGTGTGTC 
ACCTCCTGTG CCCAGCCCCA CCAGGGGCCC AGTGACTGCT GCCCTCGGTG CTCTGACTGT 
GAGCATGAGG GTCGGAAGTA TGAGCCAGGA GAGAGCTTCC AGCCCGGTGC AGACCCCTGC 
GAAGTCTGCG TCTGTGAGCT GCAGCCCGAG GGGACACCCA GCCTGCGCTG TCACCGGCGG 
CAGTGCCCCC ACCTAGCGGG CTGCCCCACC AGCCAGCTCC TGACCCCAGG GCCCCAGCAG 
TGCTGCCCCA CCTGTGCCGA GGCGCTGAGC CACTGCACAG AGCACCTGCT GGGGTCTGAG 
TTGACCCCAC CAGACCCCTG CTACAGCTGC CAGTGCCAGG ACCTGACTTG GCTCTGCATT 
CACCGGGCCT GTCCTGAGCT CAGCTGTCCC CTGCCTGAAC GCTACATACC CCCTGGGAGC 
TGCTGCCCCA TGTGCCAGGA CTGTGTGGTG GAGGCTGCGG GCCGGACAGC GGCGGATGGA 
GAGAGCTGGA GGGACCCCAG CAACGCCTGT GTCACGTGCA CCTGCCGTCG GGGCCATGTG 
GAGTGCCGCC TGGAGGAGTG CCAGGCGCTC ACCTGCCCTC GCGGCTGGGC GAAGGTGCGG 
GAGGCTGGCA GGTGCTGTGA GAGGTGCCAA GCCCCCGCCC CGTCGTGCGC GCACCAGGGC 
CGGCAGGTGG CCTCCGGGGA GCGCTGGGAC GTGGACGCCT GCACCAACTG CTCCTGCATG 
GCAGGCACCG TGCGCTGCCA GAGCCAGCGC TGCCCGCCGC TCTCCTGTGG GCCCGACGAG 
GCCCCCGCCC TGAGTCCCGG CAGCTGCTGT CCTCGCTGCC TGCCCCGGCC CGCTTCCTGC 
ATGGCCTTCG GAGACCCTCA TTACCGCACC TTCGACGGCC GCCTGCTGCA CTTCCAGGGC 
AGCTGTAGCT ACGTGCTGGC CAAGGACTGC CGTGGCGGGG ACTTCAGCGT GCACGTGACC 
AACGATGACC GGGGCCGGAG CGGCGTGTCC TGGACCCAGG AAGTGGCTGT GCTGCTGGGA 
GACGTGGCCG TGCGGCTGCT GCAGGACAGG GCGGTCACGG TGGACGGGCG CCCTGTGGCC 
TTGCCCTTCC TGCAGGAACC GCTGTTCTAT GTAGAGCTTC GGGGACGCAC GGTGGTGCTG 
CACTCCCAGC CAGGGCTCCA GGTGCTGTGG GATGGGCGGT CCCAGGTGGA GGTGAGAGTG 
CCTGGCTCCT ACCGGGGCCA CATGTGTGGG CTCTGCGGCA ACTTCAATGG CTTTGCCCAG 
GATGACCTGC GGGACCCTGA GGAACTGCTC CGGTCCACTG AGGCTGCATT TGGGAATAGC 
TGGCAGGTCC CAGAGGGCCC AGGACCTGGT CGGCCCTGTT CCAAGGGCCG TGAGGTGGAT 
CCATGCCAGG CAGCAGGGTA CCGTGCCAGG CGTGAGGCCA ACGCCCGGTG TGCGGTGCTG 
AAGTCCGCCC CGTTCAGCCG CTGCCATGCT GTGGTGCCAC CAGACCCCTT CTTTGCCGCC 
TGCGTGTATG ACCTCTGTGC TTGCGGGCCC GGTTCCTCCT CTGACGCCTG CCTCTGTGAC 
GCCCTGGAAG CCTATGCTAG CCACTGTCGC CAGGCAGGGG TGACACCAGC CTGGCGGGGC 
CCCACACTCT GCGTGGTGGG CTGTCCCCTG GACCGCGGCT TCGTGTTTGA TGAGTGTGGC 
CCACCCTGTC CCCGCACTTG CTTCAACAAG CACGTCCCCC TGGGGGAGCT GGCAGCCCAC 
TGCGTGCGGC CCTGCATCCC CAGCTGCCAG TGCCCAGCGG GCCTGGTGGA GCACGAGGGC 
CACTGTATCT CACCCGAGGC CTGCCCCTCT GTCCTGCTCA CTGGGGACCA GCTGCCCAGC 
ACTCCGCGCA ACCCCAGCCA GGAGCTTCAG GGGCCAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326211.1
CDS	770..5971
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQAASLRARSTRTGRSSRGPQAAAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470725.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470725.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGCTGCC TCTCTCAGGG CCAGGAGCAC CAGGACGGGG 
AGGAGTTCGA GGGGCCCACA GGCAGCTGCT GGCCAGGTCA GCTGTGTGCG GCAGCGCTGC 
CCACCCCTGT CCTGCCCGCT CCAGGTCACG GAGCCGGGGA GCTGCTGTCC TCGCTGCAGA 
GGCTGCCTGT TTCATGGGGA GGAGCACCCC GAAGGCAGTA GCTGGAAGCC CCCTGACAGC 
CCCTGCTCCT CCTGCATGTG TCATGAGGGT GTCATCACCT GTGCCCGCAT CCAGTGTGTC 
ACCTCCTGTG CCCAGCCCCA CCAGGGGCCC AGTGACTGCT GCCCTCGGTG CTCTGACTGT 
GAGCATGAGG GTCGGAAGTA TGAGCCAGGA GAGAGCTTCC AGCCCGGTGC AGACCCCTGC 
GAAGTCTGCG TCTGTGAGCT GCAGCCCGAG GGGACACCCA GCCTGCGCTG TCACCGGCGG 
CAGTGCCCCC ACCTAGCGGG CTGCCCCACC AGCCAGCTCC TGACCCCAGG GCCCCAGCAG 
TGCTGCCCCA CCTGTGCCGA GGCGCTGAGC CACTGCACAG AGCACCTGCT GGGGTCTGAG 
TTGACCCCAC CAGACCCCTG CTACAGCTGC CAGTGCCAGG ACCTGACTTG GCTCTGCATT 
CACCGGGCCT GTCCTGAGCT CAGCTGTCCC CTGCCTGAAC GCTACATACC CCCTGGGAGC 
TGCTGCCCCA TGTGCCAGGA CTGTGTGGTG GAGGCTGCGG GCCGGACAGC GGCGGATGGA 
GAGAGCTGGA GGGACCCCAG CAACGCCTGT GTCACGTGCA CCTGCCGTCG GGGCCATGTG 
GAGTGCCGCC TGGAGGAGTG CCAGGCGCTC ACCTGCCCTC GCGGCTGGGC GAAGGTGCGG 
GAGGCTGGCA GGTGCTGTGA GAGGTGCCAA GCCCCCGCCC CGTCGTGCGC GCACCAGGGC 
CGGCAGGTGG CCTCCGGGGA GCGCTGGGAC GTGGACGCCT GCACCAACTG CTCCTGCATG 
GCAGGCACCG TGCGCTGCCA GAGCCAGCGC TGCCCGCCGC TCTCCTGTGG GCCCGACGAG 
GCCCCCGCCC TGAGTCCCGG CAGCTGCTGT CCTCGCTGCC TGCCCCGGCC CGCTTCCTGC 
ATGGCCTTCG GAGACCCTCA TTACCGCACC TTCGACGGCC GCCTGCTGCA CTTCCAGGGC 
AGCTGTAGCT ACGTGCTGGC CAAGGACTGC CGTGGCGGGG ACTTCAGCGT GCACGTGACC 
AACGATGACC GGGGCCGGAG CGGCGTGTCC TGGACCCAGG AAGTGGCTGT GCTGCTGGGA 
GACGTGGCCG TGCGGCTGCT GCAGGACAGG GCGGTCACGG TGGACGGGCG CCCTGTGGCC 
TTGCCCTTCC TGCAGGAACC GCTGTTCTAT GTAGAGCTTC GGGGACGCAC GGTGGTGCTG 
CACTCCCAGC CAGGGCTCCA GGTGCTGTGG GATGGGCGGT CCCAGGTGGA GGTGAGAGTG 
CCTGGCTCCT ACCGGGGCCA CATGTGTGGG CTCTGCGGCA ACTTCAATGG CTTTGCCCAG 
GATGACCTGC GGGACCCTGA GGAACTGCTC CGGTCCACTG AGGCTGCATT TGGGAATAGC 
TGGCAGGTCC CAGAGGGCCC AGGACCTGGT CGGCCCTGTT CCAAGGGCCG TGAGGTGGAT 
CCATGCCAGG CAGCAGGGTA CCGTGCCAGG CGTGAGGCCA ACGCCCGGTG TGCGGTGCTG 
AAGTCCGCCC CGTTCAGCCG CTGCCATGCT GTGGTGCCAC CAGACCCCTT CTTTGCCGCC 
TGCGTGTATG ACCTCTGTGC TTGCGGGCCC GGTTCCTCCT CTGACGCCTG CCTCTGTGAC 
GCCCTGGAAG CCTATGCTAG CCACTGTCGC CAGGCAGGGG TGACACCAGC CTGGCGGGGC 
CCCACACTCT GCGTGGTGGG CTGTCCCCTG GACCGCGGCT TCGTGTTTGA TGAGTGTGGC 
CCACCCTGTC CCCGCACTTG CTTCAACAAG CACGTCCCCC TGGGGGAGCT GGCAGCCCAC 
TGCGTGCGGC CCTGCATCCC CAGCTGCCAG TGCCCAGCGG GCCTGGTGGA GCACGAGGGC 
CACTGTATCT CACCCGAGGC CTGCCCCTCT GTCCTGCTCA CTGGGGACCA GCTGCCCAGC 
ACTCCGCGCA ACCCCAGCCA GGAGCTTCAG GGGCCAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463109.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318595.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X7, mRNA.

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CloneID	OBa89766
Clone ID Related Accession (Same CDS sequence)	XM_015463109.1 , XM_015470726.1
Accession Version	XM_015463109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGCCGCCTTC CCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318595.1
CDS	778..5796
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQAAFPF

Target ORF information:

RefSeq Version	XM_015463109.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463109.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGCCGCCTTC CCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470726.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326212.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa89766
Clone ID Related Accession (Same CDS sequence)	XM_015463109.1 , XM_015470726.1
Accession Version	XM_015470726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGCCGCCTTC CCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326212.1
CDS	778..5796
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQAAFPF

Target ORF information:

RefSeq Version	XM_015470726.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470726.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGTGC TGTGGGATGG GCGGTCCCAG 
GTGGAGGTGA GAGTGCCTGG CTCCTACCGG GGCCACATGT GTGGGCTCTG CGGCAACTTC 
AATGGCTTTG CCCAGGATGA CCTGCGGGAC CCTGAGGAAC TGCTCCGGTC CACTGAGGCT 
GCATTTGGGA ATAGCTGGCA GGCCGCCTTC CCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470727.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326213.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa89767
Clone ID Related Accession (Same CDS sequence)	XM_015470727.1 , XM_015463110.1
Accession Version	XM_015470727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGAAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCCCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCAGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326213.1
CDS	1193..6157
Translation	MVAPPGGAAGKAGGRGHGAQRTGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015470727.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470727.1

ORF Insert Sequence:

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGAAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCCCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCAGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463110.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318596.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X8, mRNA.

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CloneID	OBa89767
Clone ID Related Accession (Same CDS sequence)	XM_015470727.1 , XM_015463110.1
Accession Version	XM_015463110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGAAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCCCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCAGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318596.1
CDS	1193..6157
Translation	MVAPPGGAAGKAGGRGHGAQRTGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463110.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463110.1

ORF Insert Sequence:

ATGGTGGCGC CCCCTGGAGG AGCAGCTGGA AAGGCTGGAG GCAGAGGTCA CGGAGCTCAG 
AGAACAGGCT GCAGCCACAA TGGTCAGGCC TATGGCAATG GGGAGACCTT CACCCCAGAT 
GCCTGCACCA TCTGCCGCTG CCTGGAAGGA GCTGTAACTT GCACCCAGAA GCCATGCCCG 
AGAGGACCCT GCCAAGAGCC AGGAGCATGC TGCCCACACT GCTGCCCTGG AGGCTACAGG 
GATGGGGAAA TGTGGCAATT GGAGCGCTGT GTTATCTGCA CCTGCCAGGC AGGGACAGTG 
CGGTGCCAGG GGCCCTCATG CTCAGAGCTC AACTGCCTGG AGAGCTACAC CCCACCTGGG 
GAGTGCTGCC CCGTCTGCCG GCCAGGCTGT GAGTATGAGG GGCAGCTTTA CGAGGAAGGG 
GCCAACTTCT GGTCCAGCTC CAACCCTTGT CTCCAGTGCT CCTGCCTGAG GAGCCTGGTT 
CGCTGCGTGC CCACGAAGTG CCCGCCCATC CCCTGCCCCA AGCCAGTCCT GAGGCCTGGG 
CACTGCTGCC CGACCTGCCA AGCTCAAGGC TGCACAGAAA ATGGTTCTCA CTGGGAGCAT 
GGCCAAGAGT GGACCACCCC TGGGGACCCC TGCCGGATCT GCCAGTGCCT GGAAGGCCAC 
ATCCTGTGCC GCCAGCGGGA ATGTGCCAGT CTGTGCCCAT ACCCTGCCCG GCCCCTCCCG 
GGCACCTGCT GCCCGGTGTG TGATGGCTGT TTCCTAAATG GGCGGGACTA CCGCAGCGGG 
GAGCCCGTGG GCTCTGGGGA CCCCTGCTCG CGCTGCCGTT GTGTCAATGG GAGTGTCCAG 
TGTGAGCCTC CACCCTGCCC ACCCACGCCC TGCAGACACC CAGGCAGGAC CCCGGGGGAG 
TGCTGCCCTG TCTGTGACAG CTGTGAGTAC CAGGGACACA AGTATCAGAA CCAGGAGACC 
TTCCAGCTCC AAGAGAGTGG CCGCTGTGCC CACTGCTCCT GCCAGGCTGG TGAAGTCTCC 
TGCGAGGAGC GTGAGTGCCC AGGCATGCCC TGCACCCTGC CTGACTCTGG CCCCGAGCCC 
TGCTCAGTCT GCGTGCTTGA TGAAGAGGAG TTTGCTGAGG GTGTCCAGTG GGAGCCTGAT 
GGCCAGCCCT GCACGACCTG CTCCTGTCAA GCTGGGGTAC CTGTGTGTGG GACTTTGCTC 
TGCTCCCCAG CCCCCTGCCA GCACCCCACC CAGCCCCCGG GTGCCTGCTG CCCCAGCTGT 
GAGAATTGCA CTTACCATGG CCAAGTGTAT GCCAACGGGC AGAACTTCAC AGACGCAGAC 
CCTTGCCACA CCTGCCACTG CGAGGATGGG ACCGTGACGT GCTCCTTGGT CAACTGCCCT 
CCCACAACCT GTGCCAGGCC TCAGAGGGGA CCCAGCCAAT GCTGCCCCAG GTGCCCAGAC 
TGCGTCCTGG AGAAACAGGT GTTTTTGGAC GGTGAACGCT TCTCGCACCC CCGAGATCCC 
TGCCAGGAAT GCCAGTGCCG GGAAGGCCAT GCCCACTGCC AACCCCGGCT CTGCCCCCGG 
ACCTCCTGTG CCCACCCGCT GCCTGGTGTC TGTTGCCAGA ACAACTGCAA TGGTTGTGCC 
TTTGGTGGGA AAGAGTACCC CAACGGGGCC GACTTCCCCC ACCCCTCCGA TCCCTGCCGT 
CTGTGTCACT GTCTGGGAGG CACTGTGCAG TGCCTGGCGC GCCGCTGCCC ACCCTTGCCC 
TGCCCAGAGC CGGTCCTGCT GCCCAGACAG TGCTGCCCAC GGTGCCCAGC TGCCCCCTCG 
GGCTGCCCCC GGCCAGGGGG CTTGGTCCCT GCCCACCACC AGGAGTACTT CTCCCCGCCC 
GACGACCCCT GCCGCCGCTG CCTCTGCCTG GACGGCTCGG TGTCCTGCCG GAGACTGCCC 
TGCCCTCCCG TGCCCTGCAC CCACCCGCAC CAGGGGCCCT GCTGCCCCTC CTGTGACGGC 
TGCCTGTACC AGGGGAAGGA GTTCACCAGC GGGGAGCGCT TCCCTTCGCC CACTGCCAGA 
TGTCACATCT GCCTCTGCTG GGAGGGCAGC ATCAGCTGTG AGCCCAGGGC CTGTGCACCT 
GCCCAGTGCC CCTTCCCTGC CCAGGGTGAC TGCTGCCCTG CCTGTGATGG CTGTGAGTAT 
CTGGGGGAGT CCTACCTGAG TGGCCAGGAG TTCCCAGATC CCCGCGAGCC CTGCAACCTG 
TGTACCTGCC TCGGAGGCTT CGTGACCTGC GGCCGCCGGC CCTGTGAGCC CCTGGGCTGC 
AGCCACCCAC TCACCCGAGC TGGGCACTGC TGCCCGACCT GCCAGGGATG CCTCTATCAT 
GGCGTCACTG CTGCCCCTGG AGAGACCCTT CCTGACCCAC TAGACCCCGC CTGCTCGCTC 
TGCACCTGCC AGGAAGGCTC CATGCGCTGC CAGAAGAAGC CGTGTCCTCC AGCTCTCTGC 
CCTCGCCCCT CTTCAGGACC CTGCTTCTGC CCCGTGTGCC ACAGTGAGCA CACACGCCTG 
CCCCCACGCG CTTCTCTGCC CCTCCTCCCT CCCTCTCTGC CAGGCCTGAG GTGGGACCGG 
GCAAGGTGGC TCGACCCTGC ACCCTCACCC AGGACACGTG TCGCCCACAG GCTGCCTCTC 
TCAGGGCCAG GAGCACCAGG ACGGGGAGGA GTTCGAGGGG CCCACAGGCA GCTGCTGGCC 
AGGTCAGCTG TGTGCGGCAG CGCTGCCCAC CCCTGTCCTG CCCGCTCCAG GTCACGGAGC 
CGGGGAGCTG CTGTCCTCGC TGCAGAGCAC ACACAGGGGT TCTGTCCCAG CTTCCTACTC 
CAGCCCCGAA GAAGCCCCTC CTGGTTACCC ACATGCCCTA CTGACTACGC AGAGTGGGGC 
AGGGGATCGG GGCAGACATG CAGAACCCAG ACTCAGAGCT CACCCCTGCC CACCTTGGAC 
ACAGGCTGCC TGTTTCATGG GGAGGAGCAC CCCGAAGGCA GTAGCTGGAA GCCCCCTGAC 
AGCCCCTGCT CCTCCTGCAT GTGTCATGAG GGTGTCATCA CCTGTGCCCG CATCCAGTGT 
GTCACCTCCT GTGCCCAGCC CCACCAGGGG CCCAGTGACT GCTGCCCTCG GTGCTCTGAC 
TGTGAGCATG AGGGTCGGAA GTATGAGCCA GGAGAGAGCT TCCAGCCCGG TGCAGACCCC 
TGCGAAGTCT GCGTCTGTGA GCTGCAGCCC GAGGGGACAC CCAGCCTGCG CTGTCACCGG 
CGGCAGTGCC CCCACCTAGC GGGCTGCCCC ACCAGCCAGC TCCTGACCCC AGGGCCCCAG 
CAGTGCTGCC CCACCTGTGC CGAGGCGCTG AGCCACTGCA CAGAGCACCT GCTGGGGTCT 
GAGTTGACCC CACCAGACCC CTGCTACAGC TGCCAGTGCC AGGACCTGAC TTGGCTCTGC 
ATTCACCGGG CCTGTCCTGA GCTCAGCTGT CCCCTGCCTG AACGCTACAT ACCCCCTGGG 
AGCTGCTGCC CCATGTGCCA GGACTGTGTG GTGGAGGCTG CGGGCCGGAC AGCGGCGGAT 
GGAGAGAGCT GGAGGGACCC CAGCAACGCC TGTGTCACGT GCACCTGCCG TCGGGGCCAT 
GTGGAGTGCC GCCTGGAGGA GTGCCAGGCG CTCACCTGCC CTCGCGGCTG GGCGAAGGTG 
CGGGAGGCTG GCAGGTGCTG TGAGAGGTGC CAAGCCCCCG CCCCGTCGTG CGCGCACCAG 
GGCCGGCAGG TGGCCTCCGG GGAGCGCTGG GACGTGGACG CCTGCACCAA CTGCTCCTGC 
ATGGCAGGCA CCGTGCGCTG CCAGAGCCAG CGCTGCCCGC CGCTCTCCTG TGGGCCCGAC 
GAGGCCCCCG CCCTGAGTCC CGGCAGCTGC TGTCCTCGCT GCCTGCCCCG GCCCGCTTCC 
TGCATGGCCT TCGGAGACCC TCATTACCGC ACCTTCGACG GCCGCCTGCT GCACTTCCAG 
GGCAGCTGTA GCTACGTGCT GGCCAAGGAC TGCCGTGGCG GGGACTTCAG CGTGCACGTG 
ACCAACGATG ACCGGGGCCG GAGCGGCGTG TCCTGGACCC AGGAAGTGGC TGTGCTGCTG 
GGAGACGTGG CCGTGCGGCT GCTGCAGGAC AGGGCGGTCA CGGTGGACGG GCGCCCTGTG 
GCCTTGCCCT TCCTGCAGGA ACCGCTGTTC TATGTAGAGC TTCGGGGACG CACGGTGGTG 
CTGCACTCCC AGCCAGGGCT CCAGGTGCTG TGGGATGGGC GGTCCCAGGT GGAGGTGAGA 
GTGCCTGGCT CCTACCGGGG CCACATGTGT GGGCTCTGCG GCAACTTCAA TGGCTTTGCC 
CAGGATGACC TGCGGGACCC TGAGGAACTG CTCCGGTCCA CTGAGGCTGC ATTTGGGAAT 
AGCTGGCAGG TCCCAGAGGG CCCAGGACCT GGTCGGCCCT GTTCCAAGGG CCGTGAGGTG 
GATCCATGCC AGGCAGCAGG GTACCGTGCC AGGCGTGAGG CCAACGCCCG GTGTGCGGTG 
CTGAAGTCCG CCCCGTTCAG CCGCTGCCAT GCTGTGGTGC CACCAGACCC CTTCTTTGCC 
GCCTGCGTGT ATGACCTCTG TGCTTGCGGG CCCGGTTCCT CCTCTGACGC CTGCCTCTGT 
GACGCCCTGG AAGCCTATGC TAGCCACTGT CGCCAGGCAG GGGTGACACC AGCCTGGCGG 
GGCCCCACAC TCTGCGTGGT GGGCTGTCCC CTGGACCGCG GCTTCGTGTT TGATGAGTGT 
GGCCCACCCT GTCCCCGCAC TTGCTTCAAC AAGCACGTCC CCCTGGGGGA GCTGGCAGCC 
CACTGCGTGC GGCCCTGCAT CCCCAGCTGC CAGTGCCCAG CGGGCCTGGT GGAGCACGAG 
GGCCACTGTA TCTCACCCGA GGCCTGCCCC TCTGTCCTGC TCACTGGGGA CCAGCTGCCC 
AGCACTCCGC GCAACCCCAG CCAGGAGCTT CAGGGGCCAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015470728.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326214.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X9, mRNA.

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CloneID	OBa89768
Clone ID Related Accession (Same CDS sequence)	XM_015470728.1 , XM_015463111.1
Accession Version	XM_015470728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGGGG CATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326214.1
CDS	786..5633
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQGAC

Target ORF information:

RefSeq Version	XM_015470728.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470728.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGGGG CATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463111.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318597.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X9, mRNA.

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CloneID	OBa89768
Clone ID Related Accession (Same CDS sequence)	XM_015470728.1 , XM_015463111.1
Accession Version	XM_015463111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGGGG CATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318597.1
CDS	786..5633
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSEHTRLPPRASLPLLPPSLPGLRWDRARWLDPAPSPRTRVAHRLPLSGPGAPGRGGVRGAHRQLLARSAVCGSAAHPCPARSRSRSRGAAVLAAEHTQGFCPSFLLQPRRSPSWLPTCPTDYAEWGRGSGQTCRTQTQSSPLPTLDTGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQGAC

Target ORF information:

RefSeq Version	XM_015463111.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463111.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGTGAG 
CACACACGCC TGCCCCCACG CGCTTCTCTG CCCCTCCTCC CTCCCTCTCT GCCAGGCCTG 
AGGTGGGACC GGGCAAGGTG GCTCGACCCT GCACCCTCAC CCAGGACACG TGTCGCCCAC 
AGGCTGCCTC TCTCAGGGCC AGGAGCACCA GGACGGGGAG GAGTTCGAGG GGCCCACAGG 
CAGCTGCTGG CCAGGTCAGC TGTGTGCGGC AGCGCTGCCC ACCCCTGTCC TGCCCGCTCC 
AGGTCACGGA GCCGGGGAGC TGCTGTCCTC GCTGCAGAGC ACACACAGGG GTTCTGTCCC 
AGCTTCCTAC TCCAGCCCCG AAGAAGCCCC TCCTGGTTAC CCACATGCCC TACTGACTAC 
GCAGAGTGGG GCAGGGGATC GGGGCAGACA TGCAGAACCC AGACTCAGAG CTCACCCCTG 
CCCACCTTGG ACACAGGCTG CCTGTTTCAT GGGGAGGAGC ACCCCGAAGG CAGTAGCTGG 
AAGCCCCCTG ACAGCCCCTG CTCCTCCTGC ATGTGTCATG AGGGTGTCAT CACCTGTGCC 
CGCATCCAGT GTGTCACCTC CTGTGCCCAG CCCCACCAGG GGCCCAGTGA CTGCTGCCCT 
CGGTGCTCTG ACTGTGAGCA TGAGGGTCGG AAGTATGAGC CAGGAGAGAG CTTCCAGCCC 
GGTGCAGACC CCTGCGAAGT CTGCGTCTGT GAGCTGCAGC CCGAGGGGAC ACCCAGCCTG 
CGCTGTCACC GGCGGCAGTG CCCCCACCTA GCGGGCTGCC CCACCAGCCA GCTCCTGACC 
CCAGGGCCCC AGCAGTGCTG CCCCACCTGT GCCGAGGCGC TGAGCCACTG CACAGAGCAC 
CTGCTGGGGT CTGAGTTGAC CCCACCAGAC CCCTGCTACA GCTGCCAGTG CCAGGACCTG 
ACTTGGCTCT GCATTCACCG GGCCTGTCCT GAGCTCAGCT GTCCCCTGCC TGAACGCTAC 
ATACCCCCTG GGAGCTGCTG CCCCATGTGC CAGGACTGTG TGGTGGAGGC TGCGGGCCGG 
ACAGCGGCGG ATGGAGAGAG CTGGAGGGAC CCCAGCAACG CCTGTGTCAC GTGCACCTGC 
CGTCGGGGCC ATGTGGAGTG CCGCCTGGAG GAGTGCCAGG CGCTCACCTG CCCTCGCGGC 
TGGGCGAAGG TGCGGGAGGC TGGCAGGTGC TGTGAGAGGT GCCAAGCCCC CGCCCCGTCG 
TGCGCGCACC AGGGCCGGCA GGTGGCCTCC GGGGAGCGCT GGGACGTGGA CGCCTGCACC 
AACTGCTCCT GCATGGCAGG CACCGTGCGC TGCCAGAGCC AGCGCTGCCC GCCGCTCTCC 
TGTGGGCCCG ACGAGGCCCC CGCCCTGAGT CCCGGCAGCT GCTGTCCTCG CTGCCTGCCC 
CGGCCCGCTT CCTGCATGGC CTTCGGAGAC CCTCATTACC GCACCTTCGA CGGCCGCCTG 
CTGCACTTCC AGGGCAGCTG TAGCTACGTG CTGGCCAAGG ACTGCCGTGG CGGGGACTTC 
AGCGTGCACG TGACCAACGA TGACCGGGGC CGGAGCGGCG TGTCCTGGAC CCAGGAAGTG 
GCTGTGCTGC TGGGAGACGT GGCCGTGCGG CTGCTGCAGG ACAGGGCGGT CACGGTGGAC 
GGGCGCCCTG TGGCCTTGCC CTTCCTGCAG GAACCGCTGT TCTATGTAGA GCTTCGGGGA 
CGCACGGTGG TGCTGCACTC CCAGCCAGGG CTCCAGGGGG CATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_015463107.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318593.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X5, mRNA.

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CloneID	OBa49358
Clone ID Related Accession (Same CDS sequence)	XM_015463107.1 , XM_010804494.2
Accession Version	XM_015463107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644734.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG 
TTTCATGGGG AGGAGCACCC CGAAGGCAGT AGCTGGAAGC CCCCTGACAG CCCCTGCTCC 
TCCTGCATGT GTCATGAGGG TGTCATCACC TGTGCCCGCA TCCAGTGTGT CACCTCCTGT 
GCCCAGCCCC ACCAGGGGCC CAGTGACTGC TGCCCTCGGT GCTCTGACTG TGAGCATGAG 
GGTCGGAAGT ATGAGCCAGG AGAGAGCTTC CAGCCCGGTG CAGACCCCTG CGAAGTCTGC 
GTCTGTGAGC TGCAGCCCGA GGGGACACCC AGCCTGCGCT GTCACCGGCG GCAGTGCCCC 
CACCTAGCGG GCTGCCCCAC CAGCCAGCTC CTGACCCCAG GGCCCCAGCA GTGCTGCCCC 
ACCTGTGCCG AGGCGCTGAG CCACTGCACA GAGCACCTGC TGGGGTCTGA GTTGACCCCA 
CCAGACCCCT GCTACAGCTG CCAGTGCCAG GACCTGACTT GGCTCTGCAT TCACCGGGCC 
TGTCCTGAGC TCAGCTGTCC CCTGCCTGAA CGCTACATAC CCCCTGGGAG CTGCTGCCCC 
ATGTGCCAGG ACTGTGTGGT GGAGGCTGCG GGCCGGACAG CGGCGGATGG AGAGAGCTGG 
AGGGACCCCA GCAACGCCTG TGTCACGTGC ACCTGCCGTC GGGGCCATGT GGAGTGCCGC 
CTGGAGGAGT GCCAGGCGCT CACCTGCCCT CGCGGCTGGG CGAAGGTGCG GGAGGCTGGC 
AGGTGCTGTG AGAGGTGCCA AGCCCCCGCC CCGTCGTGCG CGCACCAGGG CCGGCAGGTG 
GCCTCCGGGG AGCGCTGGGA CGTGGACGCC TGCACCAACT GCTCCTGCAT GGCAGGCACC 
GTGCGCTGCC AGAGCCAGCG CTGCCCGCCG CTCTCCTGTG GGCCCGACGA GGCCCCCGCC 
CTGAGTCCCG GCAGCTGCTG TCCTCGCTGC CTGCCCCGGC CCGCTTCCTG CATGGCCTTC 
GGAGACCCTC ATTACCGCAC CTTCGACGGC CGCCTGCTGC ACTTCCAGGG CAGCTGTAGC 
TACGTGCTGG CCAAGGACTG CCGTGGCGGG GACTTCAGCG TGCACGTGAC CAACGATGAC 
CGGGGCCGGA GCGGCGTGTC CTGGACCCAG GAAGTGGCTG TGCTGCTGGG AGACGTGGCC 
GTGCGGCTGC TGCAGGACAG GGCGGTCACG GTGGACGGGC GCCCTGTGGC CTTGCCCTTC 
CTGCAGGAAC CGCTGTTCTA TGTAGAGCTT CGGGGACGCA CGGTGGTGCT GCACTCCCAG 
CCAGGGCTCC AGGTGCTGTG GGATGGGCGG TCCCAGGTGG AGGTGAGAGT GCCTGGCTCC 
TACCGGGGCC ACATGTGTGG GCTCTGCGGC AACTTCAATG GCTTTGCCCA GGATGACCTG 
CGGGACCCTG AGGAACTGCT CCGGTCCACT GAGGCTGCAT TTGGGAATAG CTGGCAGGTC 
CCAGAGGGCC CAGGACCTGG TCGGCCCTGT TCCAAGGGCC GTGAGGTGGA TCCATGCCAG 
GCAGCAGGGT ACCGTGCCAG GCGTGAGGCC AACGCCCGGT GTGCGGTGCT GAAGTCCGCC 
CCGTTCAGCC GCTGCCATGC TGTGGTGCCA CCAGACCCCT TCTTTGCCGC CTGCGTGTAT 
GACCTCTGTG CTTGCGGGCC CGGTTCCTCC TCTGACGCCT GCCTCTGTGA CGCCCTGGAA 
GCCTATGCTA GCCACTGTCG CCAGGCAGGG GTGACACCAG CCTGGCGGGG CCCCACACTC 
TGCGTGGTGG GCTGTCCCCT GGACCGCGGC TTCGTGTTTG ATGAGTGTGG CCCACCCTGT 
CCCCGCACTT GCTTCAACAA GCACGTCCCC CTGGGGGAGC TGGCAGCCCA CTGCGTGCGG 
CCCTGCATCC CCAGCTGCCA GTGCCCAGCG GGCCTGGTGG AGCACGAGGG CCACTGTATC 
TCACCCGAGG CCTGCCCCTC TGTCCTGCTC ACTGGGGACC AGCTGCCCAG CACTCCGCGC 
AACCCCAGCC AGGAGCTTCA GGGGCCAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318593.1
CDS	769..6081
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCERCLCQAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_015463107.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463107.1

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG 
TTTCATGGGG AGGAGCACCC CGAAGGCAGT AGCTGGAAGC CCCCTGACAG CCCCTGCTCC 
TCCTGCATGT GTCATGAGGG TGTCATCACC TGTGCCCGCA TCCAGTGTGT CACCTCCTGT 
GCCCAGCCCC ACCAGGGGCC CAGTGACTGC TGCCCTCGGT GCTCTGACTG TGAGCATGAG 
GGTCGGAAGT ATGAGCCAGG AGAGAGCTTC CAGCCCGGTG CAGACCCCTG CGAAGTCTGC 
GTCTGTGAGC TGCAGCCCGA GGGGACACCC AGCCTGCGCT GTCACCGGCG GCAGTGCCCC 
CACCTAGCGG GCTGCCCCAC CAGCCAGCTC CTGACCCCAG GGCCCCAGCA GTGCTGCCCC 
ACCTGTGCCG AGGCGCTGAG CCACTGCACA GAGCACCTGC TGGGGTCTGA GTTGACCCCA 
CCAGACCCCT GCTACAGCTG CCAGTGCCAG GACCTGACTT GGCTCTGCAT TCACCGGGCC 
TGTCCTGAGC TCAGCTGTCC CCTGCCTGAA CGCTACATAC CCCCTGGGAG CTGCTGCCCC 
ATGTGCCAGG ACTGTGTGGT GGAGGCTGCG GGCCGGACAG CGGCGGATGG AGAGAGCTGG 
AGGGACCCCA GCAACGCCTG TGTCACGTGC ACCTGCCGTC GGGGCCATGT GGAGTGCCGC 
CTGGAGGAGT GCCAGGCGCT CACCTGCCCT CGCGGCTGGG CGAAGGTGCG GGAGGCTGGC 
AGGTGCTGTG AGAGGTGCCA AGCCCCCGCC CCGTCGTGCG CGCACCAGGG CCGGCAGGTG 
GCCTCCGGGG AGCGCTGGGA CGTGGACGCC TGCACCAACT GCTCCTGCAT GGCAGGCACC 
GTGCGCTGCC AGAGCCAGCG CTGCCCGCCG CTCTCCTGTG GGCCCGACGA GGCCCCCGCC 
CTGAGTCCCG GCAGCTGCTG TCCTCGCTGC CTGCCCCGGC CCGCTTCCTG CATGGCCTTC 
GGAGACCCTC ATTACCGCAC CTTCGACGGC CGCCTGCTGC ACTTCCAGGG CAGCTGTAGC 
TACGTGCTGG CCAAGGACTG CCGTGGCGGG GACTTCAGCG TGCACGTGAC CAACGATGAC 
CGGGGCCGGA GCGGCGTGTC CTGGACCCAG GAAGTGGCTG TGCTGCTGGG AGACGTGGCC 
GTGCGGCTGC TGCAGGACAG GGCGGTCACG GTGGACGGGC GCCCTGTGGC CTTGCCCTTC 
CTGCAGGAAC CGCTGTTCTA TGTAGAGCTT CGGGGACGCA CGGTGGTGCT GCACTCCCAG 
CCAGGGCTCC AGGTGCTGTG GGATGGGCGG TCCCAGGTGG AGGTGAGAGT GCCTGGCTCC 
TACCGGGGCC ACATGTGTGG GCTCTGCGGC AACTTCAATG GCTTTGCCCA GGATGACCTG 
CGGGACCCTG AGGAACTGCT CCGGTCCACT GAGGCTGCAT TTGGGAATAG CTGGCAGGTC 
CCAGAGGGCC CAGGACCTGG TCGGCCCTGT TCCAAGGGCC GTGAGGTGGA TCCATGCCAG 
GCAGCAGGGT ACCGTGCCAG GCGTGAGGCC AACGCCCGGT GTGCGGTGCT GAAGTCCGCC 
CCGTTCAGCC GCTGCCATGC TGTGGTGCCA CCAGACCCCT TCTTTGCCGC CTGCGTGTAT 
GACCTCTGTG CTTGCGGGCC CGGTTCCTCC TCTGACGCCT GCCTCTGTGA CGCCCTGGAA 
GCCTATGCTA GCCACTGTCG CCAGGCAGGG GTGACACCAG CCTGGCGGGG CCCCACACTC 
TGCGTGGTGG GCTGTCCCCT GGACCGCGGC TTCGTGTTTG ATGAGTGTGG CCCACCCTGT 
CCCCGCACTT GCTTCAACAA GCACGTCCCC CTGGGGGAGC TGGCAGCCCA CTGCGTGCGG 
CCCTGCATCC CCAGCTGCCA GTGCCCAGCG GGCCTGGTGG AGCACGAGGG CCACTGTATC 
TCACCCGAGG CCTGCCCCTC TGTCCTGCTC ACTGGGGACC AGCTGCCCAG CACTCCGCGC 
AACCCCAGCC AGGAGCTTCA GGGGCCAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCP ( XM_010804494.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802796.1 Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X5, mRNA.

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CloneID	OBa49358
Clone ID Related Accession (Same CDS sequence)	XM_015463107.1 , XM_010804494.2
Accession Version	XM_010804494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kielin/chordin-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG 
TTTCATGGGG AGGAGCACCC CGAAGGCAGT AGCTGGAAGC CCCCTGACAG CCCCTGCTCC 
TCCTGCATGT GTCATGAGGG TGTCATCACC TGTGCCCGCA TCCAGTGTGT CACCTCCTGT 
GCCCAGCCCC ACCAGGGGCC CAGTGACTGC TGCCCTCGGT GCTCTGACTG TGAGCATGAG 
GGTCGGAAGT ATGAGCCAGG AGAGAGCTTC CAGCCCGGTG CAGACCCCTG CGAAGTCTGC 
GTCTGTGAGC TGCAGCCCGA GGGGACACCC AGCCTGCGCT GTCACCGGCG GCAGTGCCCC 
CACCTAGCGG GCTGCCCCAC CAGCCAGCTC CTGACCCCAG GGCCCCAGCA GTGCTGCCCC 
ACCTGTGCCG AGGCGCTGAG CCACTGCACA GAGCACCTGC TGGGGTCTGA GTTGACCCCA 
CCAGACCCCT GCTACAGCTG CCAGTGCCAG GACCTGACTT GGCTCTGCAT TCACCGGGCC 
TGTCCTGAGC TCAGCTGTCC CCTGCCTGAA CGCTACATAC CCCCTGGGAG CTGCTGCCCC 
ATGTGCCAGG ACTGTGTGGT GGAGGCTGCG GGCCGGACAG CGGCGGATGG AGAGAGCTGG 
AGGGACCCCA GCAACGCCTG TGTCACGTGC ACCTGCCGTC GGGGCCATGT GGAGTGCCGC 
CTGGAGGAGT GCCAGGCGCT CACCTGCCCT CGCGGCTGGG CGAAGGTGCG GGAGGCTGGC 
AGGTGCTGTG AGAGGTGCCA AGCCCCCGCC CCGTCGTGCG CGCACCAGGG CCGGCAGGTG 
GCCTCCGGGG AGCGCTGGGA CGTGGACGCC TGCACCAACT GCTCCTGCAT GGCAGGCACC 
GTGCGCTGCC AGAGCCAGCG CTGCCCGCCG CTCTCCTGTG GGCCCGACGA GGCCCCCGCC 
CTGAGTCCCG GCAGCTGCTG TCCTCGCTGC CTGCCCCGGC CCGCTTCCTG CATGGCCTTC 
GGAGACCCTC ATTACCGCAC CTTCGACGGC CGCCTGCTGC ACTTCCAGGG CAGCTGTAGC 
TACGTGCTGG CCAAGGACTG CCGTGGCGGG GACTTCAGCG TGCACGTGAC CAACGATGAC 
CGGGGCCGGA GCGGCGTGTC CTGGACCCAG GAAGTGGCTG TGCTGCTGGG AGACGTGGCC 
GTGCGGCTGC TGCAGGACAG GGCGGTCACG GTGGACGGGC GCCCTGTGGC CTTGCCCTTC 
CTGCAGGAAC CGCTGTTCTA TGTAGAGCTT CGGGGACGCA CGGTGGTGCT GCACTCCCAG 
CCAGGGCTCC AGGTGCTGTG GGATGGGCGG TCCCAGGTGG AGGTGAGAGT GCCTGGCTCC 
TACCGGGGCC ACATGTGTGG GCTCTGCGGC AACTTCAATG GCTTTGCCCA GGATGACCTG 
CGGGACCCTG AGGAACTGCT CCGGTCCACT GAGGCTGCAT TTGGGAATAG CTGGCAGGTC 
CCAGAGGGCC CAGGACCTGG TCGGCCCTGT TCCAAGGGCC GTGAGGTGGA TCCATGCCAG 
GCAGCAGGGT ACCGTGCCAG GCGTGAGGCC AACGCCCGGT GTGCGGTGCT GAAGTCCGCC 
CCGTTCAGCC GCTGCCATGC TGTGGTGCCA CCAGACCCCT TCTTTGCCGC CTGCGTGTAT 
GACCTCTGTG CTTGCGGGCC CGGTTCCTCC TCTGACGCCT GCCTCTGTGA CGCCCTGGAA 
GCCTATGCTA GCCACTGTCG CCAGGCAGGG GTGACACCAG CCTGGCGGGG CCCCACACTC 
TGCGTGGTGG GCTGTCCCCT GGACCGCGGC TTCGTGTTTG ATGAGTGTGG CCCACCCTGT 
CCCCGCACTT GCTTCAACAA GCACGTCCCC CTGGGGGAGC TGGCAGCCCA CTGCGTGCGG 
CCCTGCATCC CCAGCTGCCA GTGCCCAGCG GGCCTGGTGG AGCACGAGGG CCACTGTATC 
TCACCCGAGG CCTGCCCCTC TGTCCTGCTC ACTGGGGACC AGCTGCCCAG CACTCCGCGC 
AACCCCAGCC AGGAGCTTCA GGGGCCAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802796.1
CDS	769..6081
Translation	MQEKSPPLASSFRPPRHSPAPLSRGWGQDGGAIRQAGTTGLDDRHQKPSLNKGWVGGGLVCPHPPSLCLLSQDCSQQGGESMARAVAVLLSLILQLASRTLAPGTSRGGAISREPLGLADIQDYQSEGPAHSSAPAGVPEEWWRPLEEQLERLEAEVTELREQNKDLQGRVTQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAANCVPKPGLARCHGCSHNGQAYGNGETFTPDACTICRCLEGAVTCTQKPCPRGPCQEPGACCPHCCPGGYRDGEMWQLERCVICTCQAGTVRCQGPSCSELNCLESYTPPGECCPVCRPGCEYEGQLYEEGANFWSSSNPCLQCSCLRSLVRCVPTKCPPIPCPKPVLRPGHCCPTCQAQGCTENGSHWEHGQEWTTPGDPCRICQCLEGHILCRQRECASLCPYPARPLPGTCCPVCDGCFLNGRDYRSGEPVGSGDPCSRCRCVNGSVQCEPPPCPPTPCRHPGRTPGECCPVCDSCEYQGHKYQNQETFQLQESGRCAHCSCQAGEVSCEERECPGMPCTLPDSGPEPCSVCVLDEEEFAEGVQWEPDGQPCTTCSCQAGVPVCGTLLCSPAPCQHPTQPPGACCPSCENCTYHGQVYANGQNFTDADPCHTCHCEDGTVTCSLVNCPPTTCARPQRGPSQCCPRCPDCVLEKQVFLDGERFSHPRDPCQECQCREGHAHCQPRLCPRTSCAHPLPGVCCQNNCNGCAFGGKEYPNGADFPHPSDPCRLCHCLGGTVQCLARRCPPLPCPEPVLLPRQCCPRCPAAPSGCPRPGGLVPAHHQEYFSPPDDPCRRCLCLDGSVSCRRLPCPPVPCTHPHQGPCCPSCDGCLYQGKEFTSGERFPSPTARCHICLCWEGSISCEPRACAPAQCPFPAQGDCCPACDGCEYLGESYLSGQEFPDPREPCNLCTCLGGFVTCGRRPCEPLGCSHPLTRAGHCCPTCQGCLYHGVTAAPGETLPDPLDPACSLCTCQEGSMRCQKKPCPPALCPRPSSGPCFCPVCHSCLSQGQEHQDGEEFEGPTGSCERCLCQAGQVSCVRQRCPPLSCPLQVTEPGSCCPRCRGCLFHGEEHPEGSSWKPPDSPCSSCMCHEGVITCARIQCVTSCAQPHQGPSDCCPRCSDCEHEGRKYEPGESFQPGADPCEVCVCELQPEGTPSLRCHRRQCPHLAGCPTSQLLTPGPQQCCPTCAEALSHCTEHLLGSELTPPDPCYSCQCQDLTWLCIHRACPELSCPLPERYIPPGSCCPMCQDCVVEAAGRTAADGESWRDPSNACVTCTCRRGHVECRLEECQALTCPRGWAKVREAGRCCERCQAPAPSCAHQGRQVASGERWDVDACTNCSCMAGTVRCQSQRCPPLSCGPDEAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCRGGDFSVHVTNDDRGRSGVSWTQEVAVLLGDVAVRLLQDRAVTVDGRPVALPFLQEPLFYVELRGRTVVLHSQPGLQVLWDGRSQVEVRVPGSYRGHMCGLCGNFNGFAQDDLRDPEELLRSTEAAFGNSWQVPEGPGPGRPCSKGREVDPCQAAGYRARREANARCAVLKSAPFSRCHAVVPPDPFFAACVYDLCACGPGSSSDACLCDALEAYASHCRQAGVTPAWRGPTLCVVGCPLDRGFVFDECGPPCPRTCFNKHVPLGELAAHCVRPCIPSCQCPAGLVEHEGHCISPEACPSVLLTGDQLPSTPRNPSQELQGPT

Target ORF information:

RefSeq Version	XM_010804494.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kielin/chordin-like protein (LOC515624), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804494.2

ORF Insert Sequence:

ATGCAAGAGA AGTCACCCCC ACTTGCTTCC AGCTTCCGGC CCCCCAGGCA CTCTCCTGCA 
CCTCTGAGCC GGGGCTGGGG TCAGGATGGA GGTGCCATCA GGCAGGCAGG CACCACAGGC 
CTGGATGACA GACACCAGAA GCCCTCCCTG AACAAAGGCT GGGTGGGAGG CGGCCTGGTG 
TGTCCCCACC CCCCCTCCCT GTGCCTGCTG AGCCAAGACT GCAGTCAGCA AGGCGGGGAG 
AGCATGGCCA GGGCCGTGGC CGTCCTGCTG TCCCTCATTC TGCAGCTCGC AAGCCGGACA 
CTGGCCCCGG GGACCAGCCG AGGTGGGGCC ATCTCCAGGG AGCCCCTTGG CCTTGCTGAC 
ATCCAGGACT ACCAGTCAGA GGGGCCAGCC CACTCCTCAG CCCCTGCTGG GGTCCCCGAG 
GAATGGTGGC GCCCCCTGGA GGAGCAGCTG GAAAGGCTGG AGGCAGAGGT CACGGAGCTC 
AGAGAACAGA ACAAGGACTT GCAGGGCAGG GTGACGCAGC TGGAGTCCTG TGAGTGCCAC 
CCAGCTTCCC CCCAGTGCTG GGGGTTGGGC CGGGCCTGGC CCGAGGGGGC TCGCTGGGAG 
CCCGATGCCT GCACAGCCTG TGTCTGCCAG GACGGGGCTG CAAACTGTGT CCCCAAGCCC 
GGCCTGGCCC GCTGCCACGG CTGCAGCCAC AATGGTCAGG CCTATGGCAA TGGGGAGACC 
TTCACCCCAG ATGCCTGCAC CATCTGCCGC TGCCTGGAAG GAGCTGTAAC TTGCACCCAG 
AAGCCATGCC CGAGAGGACC CTGCCAAGAG CCAGGAGCAT GCTGCCCACA CTGCTGCCCT 
GGAGGCTACA GGGATGGGGA AATGTGGCAA TTGGAGCGCT GTGTTATCTG CACCTGCCAG 
GCAGGGACAG TGCGGTGCCA GGGGCCCTCA TGCTCAGAGC TCAACTGCCT GGAGAGCTAC 
ACCCCACCTG GGGAGTGCTG CCCCGTCTGC CGGCCAGGCT GTGAGTATGA GGGGCAGCTT 
TACGAGGAAG GGGCCAACTT CTGGTCCAGC TCCAACCCTT GTCTCCAGTG CTCCTGCCTG 
AGGAGCCTGG TTCGCTGCGT GCCCACGAAG TGCCCGCCCA TCCCCTGCCC CAAGCCAGTC 
CTGAGGCCTG GGCACTGCTG CCCGACCTGC CAAGCTCAAG GCTGCACAGA AAATGGTTCT 
CACTGGGAGC ATGGCCAAGA GTGGACCACC CCTGGGGACC CCTGCCGGAT CTGCCAGTGC 
CTGGAAGGCC ACATCCTGTG CCGCCAGCGG GAATGTGCCA GTCTGTGCCC ATACCCTGCC 
CGGCCCCTCC CGGGCACCTG CTGCCCGGTG TGTGATGGCT GTTTCCTAAA TGGGCGGGAC 
TACCGCAGCG GGGAGCCCGT GGGCTCTGGG GACCCCTGCT CGCGCTGCCG TTGTGTCAAT 
GGGAGTGTCC AGTGTGAGCC TCCACCCTGC CCACCCACGC CCTGCAGACA CCCAGGCAGG 
ACCCCGGGGG AGTGCTGCCC TGTCTGTGAC AGCTGTGAGT ACCAGGGACA CAAGTATCAG 
AACCAGGAGA CCTTCCAGCT CCAAGAGAGT GGCCGCTGTG CCCACTGCTC CTGCCAGGCT 
GGTGAAGTCT CCTGCGAGGA GCGTGAGTGC CCAGGCATGC CCTGCACCCT GCCTGACTCT 
GGCCCCGAGC CCTGCTCAGT CTGCGTGCTT GATGAAGAGG AGTTTGCTGA GGGTGTCCAG 
TGGGAGCCTG ATGGCCAGCC CTGCACGACC TGCTCCTGTC AAGCTGGGGT ACCTGTGTGT 
GGGACTTTGC TCTGCTCCCC AGCCCCCTGC CAGCACCCCA CCCAGCCCCC GGGTGCCTGC 
TGCCCCAGCT GTGAGAATTG CACTTACCAT GGCCAAGTGT ATGCCAACGG GCAGAACTTC 
ACAGACGCAG ACCCTTGCCA CACCTGCCAC TGCGAGGATG GGACCGTGAC GTGCTCCTTG 
GTCAACTGCC CTCCCACAAC CTGTGCCAGG CCTCAGAGGG GACCCAGCCA ATGCTGCCCC 
AGGTGCCCAG ACTGCGTCCT GGAGAAACAG GTGTTTTTGG ACGGTGAACG CTTCTCGCAC 
CCCCGAGATC CCTGCCAGGA ATGCCAGTGC CGGGAAGGCC ATGCCCACTG CCAACCCCGG 
CTCTGCCCCC GGACCTCCTG TGCCCACCCG CTGCCTGGTG TCTGTTGCCA GAACAACTGC 
AATGGTTGTG CCTTTGGTGG GAAAGAGTAC CCCAACGGGG CCGACTTCCC CCACCCCTCC 
GATCCCTGCC GTCTGTGTCA CTGTCTGGGA GGCACTGTGC AGTGCCTGGC GCGCCGCTGC 
CCACCCTTGC CCTGCCCAGA GCCGGTCCTG CTGCCCAGAC AGTGCTGCCC ACGGTGCCCA 
GCTGCCCCCT CGGGCTGCCC CCGGCCAGGG GGCTTGGTCC CTGCCCACCA CCAGGAGTAC 
TTCTCCCCGC CCGACGACCC CTGCCGCCGC TGCCTCTGCC TGGACGGCTC GGTGTCCTGC 
CGGAGACTGC CCTGCCCTCC CGTGCCCTGC ACCCACCCGC ACCAGGGGCC CTGCTGCCCC 
TCCTGTGACG GCTGCCTGTA CCAGGGGAAG GAGTTCACCA GCGGGGAGCG CTTCCCTTCG 
CCCACTGCCA GATGTCACAT CTGCCTCTGC TGGGAGGGCA GCATCAGCTG TGAGCCCAGG 
GCCTGTGCAC CTGCCCAGTG CCCCTTCCCT GCCCAGGGTG ACTGCTGCCC TGCCTGTGAT 
GGCTGTGAGT ATCTGGGGGA GTCCTACCTG AGTGGCCAGG AGTTCCCAGA TCCCCGCGAG 
CCCTGCAACC TGTGTACCTG CCTCGGAGGC TTCGTGACCT GCGGCCGCCG GCCCTGTGAG 
CCCCTGGGCT GCAGCCACCC ACTCACCCGA GCTGGGCACT GCTGCCCGAC CTGCCAGGGA 
TGCCTCTATC ATGGCGTCAC TGCTGCCCCT GGAGAGACCC TTCCTGACCC ACTAGACCCC 
GCCTGCTCGC TCTGCACCTG CCAGGAAGGC TCCATGCGCT GCCAGAAGAA GCCGTGTCCT 
CCAGCTCTCT GCCCTCGCCC CTCTTCAGGA CCCTGCTTCT GCCCCGTGTG CCACAGCTGC 
CTCTCTCAGG GCCAGGAGCA CCAGGACGGG GAGGAGTTCG AGGGGCCCAC AGGCAGCTGT 
GAGCGCTGTC TCTGTCAGGC TGGCCAGGTC AGCTGTGTGC GGCAGCGCTG CCCACCCCTG 
TCCTGCCCGC TCCAGGTCAC GGAGCCGGGG AGCTGCTGTC CTCGCTGCAG AGGCTGCCTG 
TTTCATGGGG AGGAGCACCC CGAAGGCAGT AGCTGGAAGC CCCCTGACAG CCCCTGCTCC 
TCCTGCATGT GTCATGAGGG TGTCATCACC TGTGCCCGCA TCCAGTGTGT CACCTCCTGT 
GCCCAGCCCC ACCAGGGGCC CAGTGACTGC TGCCCTCGGT GCTCTGACTG TGAGCATGAG 
GGTCGGAAGT ATGAGCCAGG AGAGAGCTTC CAGCCCGGTG CAGACCCCTG CGAAGTCTGC 
GTCTGTGAGC TGCAGCCCGA GGGGACACCC AGCCTGCGCT GTCACCGGCG GCAGTGCCCC 
CACCTAGCGG GCTGCCCCAC CAGCCAGCTC CTGACCCCAG GGCCCCAGCA GTGCTGCCCC 
ACCTGTGCCG AGGCGCTGAG CCACTGCACA GAGCACCTGC TGGGGTCTGA GTTGACCCCA 
CCAGACCCCT GCTACAGCTG CCAGTGCCAG GACCTGACTT GGCTCTGCAT TCACCGGGCC 
TGTCCTGAGC TCAGCTGTCC CCTGCCTGAA CGCTACATAC CCCCTGGGAG CTGCTGCCCC 
ATGTGCCAGG ACTGTGTGGT GGAGGCTGCG GGCCGGACAG CGGCGGATGG AGAGAGCTGG 
AGGGACCCCA GCAACGCCTG TGTCACGTGC ACCTGCCGTC GGGGCCATGT GGAGTGCCGC 
CTGGAGGAGT GCCAGGCGCT CACCTGCCCT CGCGGCTGGG CGAAGGTGCG GGAGGCTGGC 
AGGTGCTGTG AGAGGTGCCA AGCCCCCGCC CCGTCGTGCG CGCACCAGGG CCGGCAGGTG 
GCCTCCGGGG AGCGCTGGGA CGTGGACGCC TGCACCAACT GCTCCTGCAT GGCAGGCACC 
GTGCGCTGCC AGAGCCAGCG CTGCCCGCCG CTCTCCTGTG GGCCCGACGA GGCCCCCGCC 
CTGAGTCCCG GCAGCTGCTG TCCTCGCTGC CTGCCCCGGC CCGCTTCCTG CATGGCCTTC 
GGAGACCCTC ATTACCGCAC CTTCGACGGC CGCCTGCTGC ACTTCCAGGG CAGCTGTAGC 
TACGTGCTGG CCAAGGACTG CCGTGGCGGG GACTTCAGCG TGCACGTGAC CAACGATGAC 
CGGGGCCGGA GCGGCGTGTC CTGGACCCAG GAAGTGGCTG TGCTGCTGGG AGACGTGGCC 
GTGCGGCTGC TGCAGGACAG GGCGGTCACG GTGGACGGGC GCCCTGTGGC CTTGCCCTTC 
CTGCAGGAAC CGCTGTTCTA TGTAGAGCTT CGGGGACGCA CGGTGGTGCT GCACTCCCAG 
CCAGGGCTCC AGGTGCTGTG GGATGGGCGG TCCCAGGTGG AGGTGAGAGT GCCTGGCTCC 
TACCGGGGCC ACATGTGTGG GCTCTGCGGC AACTTCAATG GCTTTGCCCA GGATGACCTG 
CGGGACCCTG AGGAACTGCT CCGGTCCACT GAGGCTGCAT TTGGGAATAG CTGGCAGGTC 
CCAGAGGGCC CAGGACCTGG TCGGCCCTGT TCCAAGGGCC GTGAGGTGGA TCCATGCCAG 
GCAGCAGGGT ACCGTGCCAG GCGTGAGGCC AACGCCCGGT GTGCGGTGCT GAAGTCCGCC 
CCGTTCAGCC GCTGCCATGC TGTGGTGCCA CCAGACCCCT TCTTTGCCGC CTGCGTGTAT 
GACCTCTGTG CTTGCGGGCC CGGTTCCTCC TCTGACGCCT GCCTCTGTGA CGCCCTGGAA 
GCCTATGCTA GCCACTGTCG CCAGGCAGGG GTGACACCAG CCTGGCGGGG CCCCACACTC 
TGCGTGGTGG GCTGTCCCCT GGACCGCGGC TTCGTGTTTG ATGAGTGTGG CCCACCCTGT 
CCCCGCACTT GCTTCAACAA GCACGTCCCC CTGGGGGAGC TGGCAGCCCA CTGCGTGCGG 
CCCTGCATCC CCAGCTGCCA GTGCCCAGCG GGCCTGGTGG AGCACGAGGG CCACTGTATC 
TCACCCGAGG CCTGCCCCTC TGTCCTGCTC ACTGGGGACC AGCTGCCCAG CACTCCGCGC 
AACCCCAGCC AGGAGCTTCA GGGGCCAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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KCP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: