PLEKHH1 cDNA ORF clone, Bos taurus(cattle)

The following PLEKHH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa23553 XM_015465197.1
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa23553 XM_005212079.3
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa23553 XM_024998096.1
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49244 XM_015465196.1
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49244 XM_010809567.3
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49244 XM_015465195.1
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49244 XM_010809568.3
Latest version!
Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa49244 XM_010809567.2 Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49244 XM_010809568.2 Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa23553
    Clone ID Related Accession (Same CDS sequence) XM_005212079.3 , XM_024998096.1 , XM_015465197.1
    Accession Version XM_005212079.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGT CCTGCCAGCT CTTCATCAAC
    GTGCCCGTGG AAGCCGCCTC GGTGGACTAC CACGTGTCCC TGGCGCAGAC GGCGCTGCAG
    GTCTGCCTGG TTCACCCCGA GCTGCAGAGC GAGATCTACT GCCAACTCAT GAAGCAGATC
    AGCTGCCGCC CGCCGCAGAA GTACTCCCTC ATGCAGTGCT GGCAGCTCCT GGCTCTGTGC
    GCCCCCCTCT TCCTGCCTCA GCACCACTTC CTCTGGTATG TCAAGCAGCA GCTCCAGCGC
    CATGCGGATC CCAGAAACGA AACTGGTCAG TACGCCACGT ACTGCCAGCG GGCGGTGGAG
    CGGACGCTGC AGACTGGGGA GCGGGAGGCC AGGCCATCGC GCATGGAGGT GGTGTCCATC
    CTGTTGCGGA ACCCCTTCCA CCACTCCCTG CCCTTCAGCA TCCCCGTGCA CTTCGCCAAT
    GGGACCTACC AGGTGGTGGG TTTTGACGGC TCCTCCACCG TGGACGAGTT CCTCCAGCGG
    CTAAACCAGG AGACGGGCAT GAGGAAGTCC TCCCATTCGG GCTTTGCCCT ATTCACGGAT
    GACCCTGCTG GCAGGGACCT GGAACACTGC CTGCAGGGGA GCGTCAAGAT CTGTGATGCC
    ATCTCCAAGT GGGAACAAGC CCTGAAGGAG CTGCACACCG GAAAGTCTGA GGGCGGCACA
    CGTGTTGTGA AGCTGATTTA CAAGAACAGG CTGTATTTTC GGAGTCAAGT CAAAGGTGAG
    ACAGAGCGAG AGCGTCTGCT GCTCGCCTCC CAAACTAATG GAGAGATAGT GGCAGGGAGG
    TTTCCTGTCA ACAAGGAGCT GGCTCTGGAG ATGGCTGCCC TGATGGCTCA GGTAGAGTAC
    GGGGACTTGG AGAGGCCCAT CCTTCCAGGA CCTGGGGGTA CACCCTCTGC CAAGGCTCAG
    CATCACCTCC AGCAGGTCCT GGACAGGTTC TACCCAAGGC GCTACAGACA CGGGGCCCCC
    CCTGAACAGC TGAGGCACCT GGCAGATCAG CTGACCACGA AGTGGGCGGC ACTGCAAGGC
    TGCTCCTCTC CTGAGTGCGT CCGCATCTAC CTGACGGTGG CCCGGAAATG GCCCCTCTTT
    GGTGCTAAGC TCTTTGCTGC TCAGCCTGCA CAGCTGTCTT CCAAGGAGAG CGCTCTGGTG
    TGGATTGCTG TGAATGAGGA TGGAGTCAGC ATCTTGGACC ACAACACTAT GCAACTGCAC
    GTCACCTATC CCTACTCTTC GGTGATGACC TTCGGTGGCT GCCGAGATGA CTTCATGCTT
    GTGATCAGAT CCGTTCCAGA CCAGAGCTCT GGGAAAGGCC ACGTTGAGAA GTTGATCTTC
    CGGATGGCTG CTCCCAAGAT TGCAGAGGTG ACCCTCATCT TGGCCAGCTA CATGAACCAC
    TGCTCCACAA CTGTGAACCC ACCCTCCACG CCCGCTGCTG CCTGCCAGCC ATGGGAACTG
    GATGGACGAC AGTTCTTTGC TTCTGTTCCC TGTGCTGCCA AGGGGCCAAC GTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212136.1
    CDS288..4367
    Translation

    Target ORF information:

    RefSeq Version XM_005212079.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212079.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGT CCTGCCAGCT CTTCATCAAC
    GTGCCCGTGG AAGCCGCCTC GGTGGACTAC CACGTGTCCC TGGCGCAGAC GGCGCTGCAG
    GTCTGCCTGG TTCACCCCGA GCTGCAGAGC GAGATCTACT GCCAACTCAT GAAGCAGATC
    AGCTGCCGCC CGCCGCAGAA GTACTCCCTC ATGCAGTGCT GGCAGCTCCT GGCTCTGTGC
    GCCCCCCTCT TCCTGCCTCA GCACCACTTC CTCTGGTATG TCAAGCAGCA GCTCCAGCGC
    CATGCGGATC CCAGAAACGA AACTGGTCAG TACGCCACGT ACTGCCAGCG GGCGGTGGAG
    CGGACGCTGC AGACTGGGGA GCGGGAGGCC AGGCCATCGC GCATGGAGGT GGTGTCCATC
    CTGTTGCGGA ACCCCTTCCA CCACTCCCTG CCCTTCAGCA TCCCCGTGCA CTTCGCCAAT
    GGGACCTACC AGGTGGTGGG TTTTGACGGC TCCTCCACCG TGGACGAGTT CCTCCAGCGG
    CTAAACCAGG AGACGGGCAT GAGGAAGTCC TCCCATTCGG GCTTTGCCCT ATTCACGGAT
    GACCCTGCTG GCAGGGACCT GGAACACTGC CTGCAGGGGA GCGTCAAGAT CTGTGATGCC
    ATCTCCAAGT GGGAACAAGC CCTGAAGGAG CTGCACACCG GAAAGTCTGA GGGCGGCACA
    CGTGTTGTGA AGCTGATTTA CAAGAACAGG CTGTATTTTC GGAGTCAAGT CAAAGGTGAG
    ACAGAGCGAG AGCGTCTGCT GCTCGCCTCC CAAACTAATG GAGAGATAGT GGCAGGGAGG
    TTTCCTGTCA ACAAGGAGCT GGCTCTGGAG ATGGCTGCCC TGATGGCTCA GGTAGAGTAC
    GGGGACTTGG AGAGGCCCAT CCTTCCAGGA CCTGGGGGTA CACCCTCTGC CAAGGCTCAG
    CATCACCTCC AGCAGGTCCT GGACAGGTTC TACCCAAGGC GCTACAGACA CGGGGCCCCC
    CCTGAACAGC TGAGGCACCT GGCAGATCAG CTGACCACGA AGTGGGCGGC ACTGCAAGGC
    TGCTCCTCTC CTGAGTGCGT CCGCATCTAC CTGACGGTGG CCCGGAAATG GCCCCTCTTT
    GGTGCTAAGC TCTTTGCTGC TCAGCCTGCA CAGCTGTCTT CCAAGGAGAG CGCTCTGGTG
    TGGATTGCTG TGAATGAGGA TGGAGTCAGC ATCTTGGACC ACAACACTAT GCAACTGCAC
    GTCACCTATC CCTACTCTTC GGTGATGACC TTCGGTGGCT GCCGAGATGA CTTCATGCTT
    GTGATCAGAT CCGTTCCAGA CCAGAGCTCT GGGAAAGGCC ACGTTGAGAA GTTGATCTTC
    CGGATGGCTG CTCCCAAGAT TGCAGAGGTG ACCCTCATCT TGGCCAGCTA CATGAACCAC
    TGCTCCACAA CTGTGAACCC ACCCTCCACG CCCGCTGCTG CCTGCCAGCC ATGGGAACTG
    GATGGACGAC AGTTCTTTGC TTCTGTTCCC TGTGCTGCCA AGGGGCCAAC GTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23553
    Clone ID Related Accession (Same CDS sequence) XM_005212079.3 , XM_024998096.1 , XM_015465197.1
    Accession Version XM_024998096.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGT CCTGCCAGCT CTTCATCAAC
    GTGCCCGTGG AAGCCGCCTC GGTGGACTAC CACGTGTCCC TGGCGCAGAC GGCGCTGCAG
    GTCTGCCTGG TTCACCCCGA GCTGCAGAGC GAGATCTACT GCCAACTCAT GAAGCAGATC
    AGCTGCCGCC CGCCGCAGAA GTACTCCCTC ATGCAGTGCT GGCAGCTCCT GGCTCTGTGC
    GCCCCCCTCT TCCTGCCTCA GCACCACTTC CTCTGGTATG TCAAGCAGCA GCTCCAGCGC
    CATGCGGATC CCAGAAACGA AACTGGTCAG TACGCCACGT ACTGCCAGCG GGCGGTGGAG
    CGGACGCTGC AGACTGGGGA GCGGGAGGCC AGGCCATCGC GCATGGAGGT GGTGTCCATC
    CTGTTGCGGA ACCCCTTCCA CCACTCCCTG CCCTTCAGCA TCCCCGTGCA CTTCGCCAAT
    GGGACCTACC AGGTGGTGGG TTTTGACGGC TCCTCCACCG TGGACGAGTT CCTCCAGCGG
    CTAAACCAGG AGACGGGCAT GAGGAAGTCC TCCCATTCGG GCTTTGCCCT ATTCACGGAT
    GACCCTGCTG GCAGGGACCT GGAACACTGC CTGCAGGGGA GCGTCAAGAT CTGTGATGCC
    ATCTCCAAGT GGGAACAAGC CCTGAAGGAG CTGCACACCG GAAAGTCTGA GGGCGGCACA
    CGTGTTGTGA AGCTGATTTA CAAGAACAGG CTGTATTTTC GGAGTCAAGT CAAAGGTGAG
    ACAGAGCGAG AGCGTCTGCT GCTCGCCTCC CAAACTAATG GAGAGATAGT GGCAGGGAGG
    TTTCCTGTCA ACAAGGAGCT GGCTCTGGAG ATGGCTGCCC TGATGGCTCA GGTAGAGTAC
    GGGGACTTGG AGAGGCCCAT CCTTCCAGGA CCTGGGGGTA CACCCTCTGC CAAGGCTCAG
    CATCACCTCC AGCAGGTCCT GGACAGGTTC TACCCAAGGC GCTACAGACA CGGGGCCCCC
    CCTGAACAGC TGAGGCACCT GGCAGATCAG CTGACCACGA AGTGGGCGGC ACTGCAAGGC
    TGCTCCTCTC CTGAGTGCGT CCGCATCTAC CTGACGGTGG CCCGGAAATG GCCCCTCTTT
    GGTGCTAAGC TCTTTGCTGC TCAGCCTGCA CAGCTGTCTT CCAAGGAGAG CGCTCTGGTG
    TGGATTGCTG TGAATGAGGA TGGAGTCAGC ATCTTGGACC ACAACACTAT GCAACTGCAC
    GTCACCTATC CCTACTCTTC GGTGATGACC TTCGGTGGCT GCCGAGATGA CTTCATGCTT
    GTGATCAGAT CCGTTCCAGA CCAGAGCTCT GGGAAAGGCC ACGTTGAGAA GTTGATCTTC
    CGGATGGCTG CTCCCAAGAT TGCAGAGGTG ACCCTCATCT TGGCCAGCTA CATGAACCAC
    TGCTCCACAA CTGTGAACCC ACCCTCCACG CCCGCTGCTG CCTGCCAGCC ATGGGAACTG
    GATGGACGAC AGTTCTTTGC TTCTGTTCCC TGTGCTGCCA AGGGGCCAAC GTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853864.1
    CDS148..4227
    Translation

    Target ORF information:

    RefSeq Version XM_024998096.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGT CCTGCCAGCT CTTCATCAAC
    GTGCCCGTGG AAGCCGCCTC GGTGGACTAC CACGTGTCCC TGGCGCAGAC GGCGCTGCAG
    GTCTGCCTGG TTCACCCCGA GCTGCAGAGC GAGATCTACT GCCAACTCAT GAAGCAGATC
    AGCTGCCGCC CGCCGCAGAA GTACTCCCTC ATGCAGTGCT GGCAGCTCCT GGCTCTGTGC
    GCCCCCCTCT TCCTGCCTCA GCACCACTTC CTCTGGTATG TCAAGCAGCA GCTCCAGCGC
    CATGCGGATC CCAGAAACGA AACTGGTCAG TACGCCACGT ACTGCCAGCG GGCGGTGGAG
    CGGACGCTGC AGACTGGGGA GCGGGAGGCC AGGCCATCGC GCATGGAGGT GGTGTCCATC
    CTGTTGCGGA ACCCCTTCCA CCACTCCCTG CCCTTCAGCA TCCCCGTGCA CTTCGCCAAT
    GGGACCTACC AGGTGGTGGG TTTTGACGGC TCCTCCACCG TGGACGAGTT CCTCCAGCGG
    CTAAACCAGG AGACGGGCAT GAGGAAGTCC TCCCATTCGG GCTTTGCCCT ATTCACGGAT
    GACCCTGCTG GCAGGGACCT GGAACACTGC CTGCAGGGGA GCGTCAAGAT CTGTGATGCC
    ATCTCCAAGT GGGAACAAGC CCTGAAGGAG CTGCACACCG GAAAGTCTGA GGGCGGCACA
    CGTGTTGTGA AGCTGATTTA CAAGAACAGG CTGTATTTTC GGAGTCAAGT CAAAGGTGAG
    ACAGAGCGAG AGCGTCTGCT GCTCGCCTCC CAAACTAATG GAGAGATAGT GGCAGGGAGG
    TTTCCTGTCA ACAAGGAGCT GGCTCTGGAG ATGGCTGCCC TGATGGCTCA GGTAGAGTAC
    GGGGACTTGG AGAGGCCCAT CCTTCCAGGA CCTGGGGGTA CACCCTCTGC CAAGGCTCAG
    CATCACCTCC AGCAGGTCCT GGACAGGTTC TACCCAAGGC GCTACAGACA CGGGGCCCCC
    CCTGAACAGC TGAGGCACCT GGCAGATCAG CTGACCACGA AGTGGGCGGC ACTGCAAGGC
    TGCTCCTCTC CTGAGTGCGT CCGCATCTAC CTGACGGTGG CCCGGAAATG GCCCCTCTTT
    GGTGCTAAGC TCTTTGCTGC TCAGCCTGCA CAGCTGTCTT CCAAGGAGAG CGCTCTGGTG
    TGGATTGCTG TGAATGAGGA TGGAGTCAGC ATCTTGGACC ACAACACTAT GCAACTGCAC
    GTCACCTATC CCTACTCTTC GGTGATGACC TTCGGTGGCT GCCGAGATGA CTTCATGCTT
    GTGATCAGAT CCGTTCCAGA CCAGAGCTCT GGGAAAGGCC ACGTTGAGAA GTTGATCTTC
    CGGATGGCTG CTCCCAAGAT TGCAGAGGTG ACCCTCATCT TGGCCAGCTA CATGAACCAC
    TGCTCCACAA CTGTGAACCC ACCCTCCACG CCCGCTGCTG CCTGCCAGCC ATGGGAACTG
    GATGGACGAC AGTTCTTTGC TTCTGTTCCC TGTGCTGCCA AGGGGCCAAC GTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23553
    Clone ID Related Accession (Same CDS sequence) XM_005212079.3 , XM_024998096.1 , XM_015465197.1
    Accession Version XM_015465197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGT CCTGCCAGCT CTTCATCAAC
    GTGCCCGTGG AAGCCGCCTC GGTGGACTAC CACGTGTCCC TGGCGCAGAC GGCGCTGCAG
    GTCTGCCTGG TTCACCCCGA GCTGCAGAGC GAGATCTACT GCCAACTCAT GAAGCAGATC
    AGCTGCCGCC CGCCGCAGAA GTACTCCCTC ATGCAGTGCT GGCAGCTCCT GGCTCTGTGC
    GCCCCCCTCT TCCTGCCTCA GCACCACTTC CTCTGGTATG TCAAGCAGCA GCTCCAGCGC
    CATGCGGATC CCAGAAACGA AACTGGTCAG TACGCCACGT ACTGCCAGCG GGCGGTGGAG
    CGGACGCTGC AGACTGGGGA GCGGGAGGCC AGGCCATCGC GCATGGAGGT GGTGTCCATC
    CTGTTGCGGA ACCCCTTCCA CCACTCCCTG CCCTTCAGCA TCCCCGTGCA CTTCGCCAAT
    GGGACCTACC AGGTGGTGGG TTTTGACGGC TCCTCCACCG TGGACGAGTT CCTCCAGCGG
    CTAAACCAGG AGACGGGCAT GAGGAAGTCC TCCCATTCGG GCTTTGCCCT ATTCACGGAT
    GACCCTGCTG GCAGGGACCT GGAACACTGC CTGCAGGGGA GCGTCAAGAT CTGTGATGCC
    ATCTCCAAGT GGGAACAAGC CCTGAAGGAG CTGCACACCG GAAAGTCTGA GGGCGGCACA
    CGTGTTGTGA AGCTGATTTA CAAGAACAGG CTGTATTTTC GGAGTCAAGT CAAAGGTGAG
    ACAGAGCGAG AGCGTCTGCT GCTCGCCTCC CAAACTAATG GAGAGATAGT GGCAGGGAGG
    TTTCCTGTCA ACAAGGAGCT GGCTCTGGAG ATGGCTGCCC TGATGGCTCA GGTAGAGTAC
    GGGGACTTGG AGAGGCCCAT CCTTCCAGGA CCTGGGGGTA CACCCTCTGC CAAGGCTCAG
    CATCACCTCC AGCAGGTCCT GGACAGGTTC TACCCAAGGC GCTACAGACA CGGGGCCCCC
    CCTGAACAGC TGAGGCACCT GGCAGATCAG CTGACCACGA AGTGGGCGGC ACTGCAAGGC
    TGCTCCTCTC CTGAGTGCGT CCGCATCTAC CTGACGGTGG CCCGGAAATG GCCCCTCTTT
    GGTGCTAAGC TCTTTGCTGC TCAGCCTGCA CAGCTGTCTT CCAAGGAGAG CGCTCTGGTG
    TGGATTGCTG TGAATGAGGA TGGAGTCAGC ATCTTGGACC ACAACACTAT GCAACTGCAC
    GTCACCTATC CCTACTCTTC GGTGATGACC TTCGGTGGCT GCCGAGATGA CTTCATGCTT
    GTGATCAGAT CCGTTCCAGA CCAGAGCTCT GGGAAAGGCC ACGTTGAGAA GTTGATCTTC
    CGGATGGCTG CTCCCAAGAT TGCAGAGGTG ACCCTCATCT TGGCCAGCTA CATGAACCAC
    TGCTCCACAA CTGTGAACCC ACCCTCCACG CCCGCTGCTG CCTGCCAGCC ATGGGAACTG
    GATGGACGAC AGTTCTTTGC TTCTGTTCCC TGTGCTGCCA AGGGGCCAAC GTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320683.1
    CDS288..4367
    Translation

    Target ORF information:

    RefSeq Version XM_015465197.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGT CCTGCCAGCT CTTCATCAAC
    GTGCCCGTGG AAGCCGCCTC GGTGGACTAC CACGTGTCCC TGGCGCAGAC GGCGCTGCAG
    GTCTGCCTGG TTCACCCCGA GCTGCAGAGC GAGATCTACT GCCAACTCAT GAAGCAGATC
    AGCTGCCGCC CGCCGCAGAA GTACTCCCTC ATGCAGTGCT GGCAGCTCCT GGCTCTGTGC
    GCCCCCCTCT TCCTGCCTCA GCACCACTTC CTCTGGTATG TCAAGCAGCA GCTCCAGCGC
    CATGCGGATC CCAGAAACGA AACTGGTCAG TACGCCACGT ACTGCCAGCG GGCGGTGGAG
    CGGACGCTGC AGACTGGGGA GCGGGAGGCC AGGCCATCGC GCATGGAGGT GGTGTCCATC
    CTGTTGCGGA ACCCCTTCCA CCACTCCCTG CCCTTCAGCA TCCCCGTGCA CTTCGCCAAT
    GGGACCTACC AGGTGGTGGG TTTTGACGGC TCCTCCACCG TGGACGAGTT CCTCCAGCGG
    CTAAACCAGG AGACGGGCAT GAGGAAGTCC TCCCATTCGG GCTTTGCCCT ATTCACGGAT
    GACCCTGCTG GCAGGGACCT GGAACACTGC CTGCAGGGGA GCGTCAAGAT CTGTGATGCC
    ATCTCCAAGT GGGAACAAGC CCTGAAGGAG CTGCACACCG GAAAGTCTGA GGGCGGCACA
    CGTGTTGTGA AGCTGATTTA CAAGAACAGG CTGTATTTTC GGAGTCAAGT CAAAGGTGAG
    ACAGAGCGAG AGCGTCTGCT GCTCGCCTCC CAAACTAATG GAGAGATAGT GGCAGGGAGG
    TTTCCTGTCA ACAAGGAGCT GGCTCTGGAG ATGGCTGCCC TGATGGCTCA GGTAGAGTAC
    GGGGACTTGG AGAGGCCCAT CCTTCCAGGA CCTGGGGGTA CACCCTCTGC CAAGGCTCAG
    CATCACCTCC AGCAGGTCCT GGACAGGTTC TACCCAAGGC GCTACAGACA CGGGGCCCCC
    CCTGAACAGC TGAGGCACCT GGCAGATCAG CTGACCACGA AGTGGGCGGC ACTGCAAGGC
    TGCTCCTCTC CTGAGTGCGT CCGCATCTAC CTGACGGTGG CCCGGAAATG GCCCCTCTTT
    GGTGCTAAGC TCTTTGCTGC TCAGCCTGCA CAGCTGTCTT CCAAGGAGAG CGCTCTGGTG
    TGGATTGCTG TGAATGAGGA TGGAGTCAGC ATCTTGGACC ACAACACTAT GCAACTGCAC
    GTCACCTATC CCTACTCTTC GGTGATGACC TTCGGTGGCT GCCGAGATGA CTTCATGCTT
    GTGATCAGAT CCGTTCCAGA CCAGAGCTCT GGGAAAGGCC ACGTTGAGAA GTTGATCTTC
    CGGATGGCTG CTCCCAAGAT TGCAGAGGTG ACCCTCATCT TGGCCAGCTA CATGAACCAC
    TGCTCCACAA CTGTGAACCC ACCCTCCACG CCCGCTGCTG CCTGCCAGCC ATGGGAACTG
    GATGGACGAC AGTTCTTTGC TTCTGTTCCC TGTGCTGCCA AGGGGCCAAC GTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49244
    Clone ID Related Accession (Same CDS sequence) XM_015465195.1 , XM_015465196.1 , XM_010809567.2 , XM_010809568.3 , XM_010809567.3 , XM_010809568.2
    Accession Version XM_015465195.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320681.1
    CDS291..4439
    Translation

    Target ORF information:

    RefSeq Version XM_015465195.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49244
    Clone ID Related Accession (Same CDS sequence) XM_015465195.1 , XM_015465196.1 , XM_010809567.2 , XM_010809568.3 , XM_010809567.3 , XM_010809568.2
    Accession Version XM_015465196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320682.1
    CDS146..4294
    Translation

    Target ORF information:

    RefSeq Version XM_015465196.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49244
    Clone ID Related Accession (Same CDS sequence) XM_015465195.1 , XM_015465196.1 , XM_010809567.2 , XM_010809568.3 , XM_010809567.3 , XM_010809568.2
    Accession Version XM_010809567.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807869.1
    CDS291..4439
    Translation

    Target ORF information:

    RefSeq Version XM_010809567.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809567.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49244
    Clone ID Related Accession (Same CDS sequence) XM_015465195.1 , XM_015465196.1 , XM_010809567.2 , XM_010809568.3 , XM_010809567.3 , XM_010809568.2
    Accession Version XM_010809568.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807870.1
    CDS149..4297
    Translation

    Target ORF information:

    RefSeq Version XM_010809568.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809568.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49244
    Clone ID Related Accession (Same CDS sequence) XM_015465195.1 , XM_015465196.1 , XM_010809567.2 , XM_010809568.3 , XM_010809567.3 , XM_010809568.2
    Accession Version XM_010809567.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807869.1
    CDS290..4438
    Translation

    Target ORF information:

    RefSeq Version XM_010809567.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809567.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49244
    Clone ID Related Accession (Same CDS sequence) XM_015465195.1 , XM_015465196.1 , XM_010809567.2 , XM_010809568.3 , XM_010809567.3 , XM_010809568.2
    Accession Version XM_010809568.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family H member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807870.1
    CDS146..4294
    Translation

    Target ORF information:

    RefSeq Version XM_010809568.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology, MyTH4 and FERM domain containing H1 (PLEKHH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809568.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCAGAAC TCACGGTGGA GACGCGGGCC AGTGTGGACT GGCAGAAGCG CTGCTTGGCC 
    CTAGAAACCC AGCTTTTCCG GTTCCGACTG CAGGCCAGCA AGATCAGAGA GCTGCTGGCC
    GACAAGATGC AGGAGCTGGA GCAGAGGCTG CTGGACGCAG AGCAGAGAGC AGAGAACGCG
    GAGACCCAGG TGGGTGTGAT GGAAGAGAAG GTGAAATTGT CCAATCTGAT GAATGTGGAC
    TCAGCAGGCA GCCTGCACCG GAAGTACCAA GAGTTGCTGA AAGCCATGCA GGGCAAAGAT
    GAGCTCATCA GCGAGCTGGA GGCACAGCTG GAGAAGCAGA AGCAGATGAG AGCAGAAGAG
    GCAAAAGTTG TTCAAGAAAA AGCTGCAAAG ATCAAGGAAT GGGTGACACT GAAATTAGCA
    GAACTTGAGA TGGAGAATCA GCACCTGAAA AGCTGTAATC AGCACCTGGT GGAGCAGGTG
    GGAGCCCTTC AACGTGCAGT GGAAGCTCTT CAGATGTCAC CTTCGGGGAA GCTGCTGGTG
    GCCCCCCAGG GAACCACAGA GCAGGAGTCT GTCCCTTCGG GACCAGGGAC CCAGCCAGCG
    GGGCAGGACA GTGGTCCTCT GGCCCAGGGT GCCCTGAAGG CAGCTATCCC TGCACCTTCT
    CCAGGTGCCC TGCAGAGCAA GGACTCTGTT CCTAAAGCAG GAAGCCCCAT GGAGGATTCT
    AGTTCCCAGA CAGTCCACCC TGGAGCAACA TCAGAGGCCA AGACCCTTCC ACTTCATCTG
    GGAAGACAGG GCTCTCCTGG CCAGCTGTGC CTGAGGCCTC GTACCCCCAG GCATGCTCTG
    GCTTCCTGGG GTGAGGGCCT GGTCACTGCT CAGGGAGGGA CGCTCCCTCG GACGAAGACT
    TCTGCCAAGG AAGGTGGCCC CGGCTGTAGC CTGACCCTGC CAAAGGCCCG GGCTCCCAGC
    ACCCTCCGGG ACAGCATCCA GCTGGCCAAG CGGCACCACA GCCAGCCTCA GGCAGGCCCT
    GGGCACTTCA GCCATGTGGT GCGCATTGAG GTGGGGACCC TCTCAGCCTT CCACCCCCAC
    AGCCTTCCCA AGGGGGTGGT CAGAGCCAAG CTCCGGGAGG AACCAGAGAA GATGGAGATG
    GAGGAACAAC CCCCTGTAGG GGAGAAGGAG GCCCAAGGAA CAGAGCTGGA GGAAGTGGAT
    TTGGAGAACA AACCACCCAC ACCCCCCTTG CACCGCTTTC CATCCTGGGA GAGCCGGATC
    TACGCTGTGG CCACGTCCGG CATGCGGCTG TCTGAAGTGC CTGTCAGAAG TAATGCCACC
    TGCTGCGCTT CAAGCCCTCC TGCTCTGGCA TCACCTGGGC CATTCTCTGG CCTTGTCTAC
    AAGAACGTCG CCGTGCCTGT CTACACAGCC CTGAAGGGGA AAGCCACGCA GATCAGCACG
    GTGCCCTTTG TGGACGAGTC CTCTGGGTCC GATGATGACT GCAGCTCCCA GGCTAGCTTC
    CGAACTTCAC TCCCCTGCTC TGAGTCCAGG AAAACCAGTG GACTGGGCAG CCCCCGGGCC
    ATCAAGAGAG GCGTCTCCGT GTCCTCGCTG AGCTCCGAGG GTGACTACGC CATTCCCCCG
    GATGCCTGCT CCTTGGACAG TGACTACTCT GAGCCTGAGC ACAAACTGCA GCGCACCTCA
    TCCTATTCCA CTGATGGGCT GGGCCCGGGC GGGGAGTCAC TGGAGAAGTC GGGCTACTTG
    CTGAAAATGG GGAGCCGGGT GAAGACGTGG AAGAGGCGCT GGTTTGTGCT GAGACAGGGA
    CAGATCATGT ACTACAAGTC TCCGAGTGAT GTCATCCGGA AACCGCAAGG CCAAGTGGAA
    CTAAACTCCC GTTGCCAGAT TGTTCGAGGG GAAGGTGCAC AGACATTCCA GCTCATCTCT
    GAGAAGAAGA CCTACTACCT GACGGCTGAC TCGCCTAGCC TGCTGGAGGA GTGGATCCGT
    GTCCTGCAGA GCCTGCTGAA GGTCCAGGCC ATTGGGCCTC CAGCCCTGCC TCAGGGGGGC
    ACCAAGCCCA CCGTGAAGGG CTGGCTGACT AAGGTCAAGC ATGGCCACTC CAAGCTGGTC
    TGGTGTGCTC TTGTTGGGAG AACCTTCTAC TACTATCGAA GCCATGAGGA CAAGCGACCC
    CTGGGCCGTC TGCCTGTGCG GGACGCGCGC ATAGAGGAAG TAGATCGATC CTGCGACTCA
    GACGAGGACT ATGAGGCTGG AGGAACCCGG CGGTTGCTGT CCTCCCACTA CACGCTGGTG
    ATCCACCCCC CGGAGCACAG CCCCACCTAC CTCCTCATTG GCACCAAGCA TGAAAAGGAT
    ACGTGGCTTT ACCACCTCAC GGTGGCTGCA GGTGGCAGCA GTGCCAAGGT GGGCACTGCC
    TATGAGCAGC TCATTGGAAA GCTGATGGAT GGTGAAGGAG ACCCGGACTC CCCCCTCTGG
    AGGCACCCCA TGCTGTGCTA CAGCAAAGAC GGGCTGTACA CCTCCCTCAC CACCCTGCCC
    TCCGAGGCCC TGCAGACGGA AGCTGTCAAG CTCTTCAAGG CCAGTCTGTC CCAGCCACAG
    AGGACAGTCG TCAAGGAACC GCCCGAGCCT GCCGTCGTCT CCCTGCAGTC CTGCCAGCTC
    TTCATCAACG TGCCCGTGGA AGCCGCCTCG GTGGACTACC ACGTGTCCCT GGCGCAGACG
    GCGCTGCAGG TCTGCCTGGT TCACCCCGAG CTGCAGAGCG AGATCTACTG CCAACTCATG
    AAGCAGATCA GCTGCCGCCC GCCGCAGAAG TACTCCCTCA TGCAGTGCTG GCAGCTCCTG
    GCTCTGTGCG CCCCCCTCTT CCTGCCTCAG CACCACTTCC TCTGGTATGT CAAGCAGCAG
    CTCCAGCGCC ATGCGGATCC CAGAAACGAA ACTGGTCAGT ACGCCACGTA CTGCCAGCGG
    GCGGTGGAGC GGACGCTGCA GACTGGGGAG CGGGAGGCCA GGCCATCGCG CATGGAGGTG
    GTGTCCATCC TGTTGCGGAA CCCCTTCCAC CACTCCCTGC CCTTCAGCAT CCCCGTGCAC
    TTCGCCAATG GGACCTACCA GGTGGTGGGT TTTGACGGCT CCTCCACCGT GGACGAGTTC
    CTCCAGCGGC TAAACCAGGA GACGGGCATG AGGAAGTCCT CCCATTCGGG CTTTGCCCTA
    TTCACGGATG ACCCTGCTGG CAGGGACCTG GAACACTGCC TGCAGGGGAG CGTCAAGATC
    TGTGATGCCA TCTCCAAGTG GGAACAAGCC CTGAAGGAGC TGCACACCGG AAAGTCTGAG
    GGCGGCACAC GTGTTGTGAA GCTGATTTAC AAGAACAGGC TGTATTTTCG GAGTCAAGTC
    AAAGGTGAGA CAGAGCGAGA GCGTCTGCTG CTCGCCTCCC AAACTAATGG AGAGATAGTG
    GCAGGGAGGT TTCCTGTCAA CAAGGAGCTG GCTCTGGAGA TGGCTGCCCT GATGGCTCAG
    GTAGAGTACG GGGACTTGGA GAGGCCCATC CTTCCAGGAC CTGGGGGTAC ACCCTCTGCC
    AAGGCTCAGC ATCACCTCCA GCAGGTCCTG GACAGGTTCT ACCCAAGGCG CTACAGACAC
    GGGGCCCCCC CTGAACAGCT GAGGCACCTG GCAGATCAGC TGACCACGAA GTGGGCGGCA
    CTGCAAGGCT GCTCCTCTCC TGAGTGCGTC CGCATCTACC TGACGGTGGC CCGGAAATGG
    CCCCTCTTTG GTGCTAAGCT CTTTGCTGCT CAGCCTGCAC AGCTGTCTTC CAAGGAGAGC
    GCTCTGGTGT GGATTGCTGT GAATGAGGAT GGAGTCAGCA TCTTGGACCA CAACACTATG
    CAACTGCACG TCACCTATCC CTACTCTTCG GTGATGACCT TCGGTGGCTG CCGAGATGAC
    TTCATGCTTG TGATCAGATC CGTTCCAGAC CAGAGCTCTG GGAAAGGCCA CGTTGAGAAG
    TTGATCTTCC GGATGGCTGC TCCCAAGATT GCAGAGGTGA CCCTCATCTT GGCCAGCTAC
    ATGAACCACT GCTCCACAAC TGTGAACCCA CCCTCCACGC CCGCTGCTGC CTGCCAGCCA
    TGGGAACTGG ATGGACGACA GTTCTTTGCT TCTGTTCCCT GTGCTGCCAA GGGGCCAACG
    TTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.