METTL25 cDNA ORF clone, Bos taurus(cattle)

The following METTL25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the METTL25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa19076 XM_010804816.2
Latest version!
Bos taurus methyltransferase like 25 (METTL25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa04172 XM_024992400.1
Latest version!
Bos taurus methyltransferase like 25 (METTL25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBa04172 XM_024992401.1
Latest version!
Bos taurus methyltransferase like 25 (METTL25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBa89847 XM_015463258.1
Latest version!
Bos taurus methyltransferase like 25 (METTL25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa19076
    Clone ID Related Accession (Same CDS sequence) XM_010804816.2
    Accession Version XM_010804816.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methyltransferase-like protein 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103916.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGGTGAAT GGCGGGTGGG CGGGGCGGGC AAGAAGGCGG GAAGAGACCC CGGGCCGTGG
    GTAGTGTCCC CGGTCGCGGA AGTCGAGCTG AGGGCTGTGC CGGCGGGGAG CGGGCCAGGC
    AGATCGCTGC CGTGTGGGTC AGCAATTCAG GAGCAGGTGG ACTGGCGGAT CAAGCTTAGG
    GTCTCATGGT TTCACAGTCA AGATGTTACA GTTGCTGAAG GCTTGATGGA AATTGGGGAA
    CTTGCCTCCA CAGCGTCTCA CTTACATAGG GAAGAGTGTG ATCTGTGTGC CAGTGCCAGC
    GCAGCTATGA GTTGCTCTCG ATCACAGTTG ATTGCTATTA TAATTGACCT GGGTTCTGGA
    AAAGGCTACC TAAGCTCCTT TTTGTCCTTA AAGTATGGCT TAAAAGTTTA TGGAATCGAT
    TCCTCAAATA CTCGTGGAGC TGAGGAGAGA AACAGGAAAC TGAAGAAACA TTGGAAAGTC
    TATGGTAGGC GGTCAAAACC AGATGTCAGT GGACTGGCAT TACAGACAAC GAAAGAAAGG
    AAAGTGCAAG ATGAAACTAA AGCAGACATT GAGGGAATAT GTAACAGCAG TACTGCAAGT
    CAAGAGAAGC CACCAGCCTC AGACTTTTTA TCAGAGTTTT CAGGTTCTGT GATTTCTGAC
    ATCAGAAGAC AAATGGAAAA CCTAAAGGTT TATTCACACC GAGAGGAAAA TTTGTGTTTT
    GAAAATGCCT TTTCTCTTAG AGACCTTTTG CCTGTTAATG CCATAGAACC TAATTCCTCA
    TCCCAAATAC CTAAGAGAAA AATGTCTGAA GCAAGTAAAG AGAGAAGAAA AATTACTTTG
    AAGTCAAATG AATCAAATAT ATATTCACCT TTAACATCTA TTATCACTGC TGATTCAGAA
    CTACATGATA TTATTAAAGA TCTGGAGGAT TGTTTGATGG TAGGTCTACA TACTTGTGGT
    GATCTTGCTC CAAATACTCT GCGAATATTC ACTTCCAAGT CTGAAGTCAA AGGAGTTTGC
    AGTGTGGGTT GCTGCTATCA CCTCTTATCT GAAGAGTTTG AAAACCTACA TAAAGAATAT
    ACCCAAGAAA GGCGGGGATT TCCAATGTGC CACTATTTGA AGGAAGAGAG ATGGTGCTGT
    GGCCGAAACG CCAGAATGTC AGCCTGTTTG GCTCTGGAGC GGGTTGCAGT TGGCCAAGGG
    CTGCCTACTG AGTCCCTCTT CTATCGTGCC ATACTCCAGG ATATTATTAA AGAGTGCTAT
    GGCATCACCA AATGTGACCG GCATGTTGGT AAAATTTATT CCAAATCCTC CTCCTTTCCG
    GATTATGTCA GAAAGTCTCT AAAGAAGCTT GGGTTGGATG AGTCCAAGCT GCCAGAAAAA
    ATTATAATGG ACTACTACGA GAAATATAAG CCTAGAATGA ATGAACTGGA GGCTTTTAAT
    ATGTTGAAAG TTGTTCTAGC TCCTTGCATA GAGACCTTGA TTCTTTTGGA TCGACTTTGC
    TACCTGAAAG AGCAGGATGA TATCATGTGG TCTGCGCTTG TGAGGTTGTT TGATCCTGTG
    AAATCTCCCA GATGTTATGC CATTATTGCC CTGAAGAAGC AGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803118.1
    CDS1..1848
    Translation

    Target ORF information:

    RefSeq Version XM_010804816.2
    Organism Bos taurus(cattle)
    Definition Bos taurus methyltransferase like 25 (METTL25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804816.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGGTGAAT GGCGGGTGGG CGGGGCGGGC AAGAAGGCGG GAAGAGACCC CGGGCCGTGG
    GTAGTGTCCC CGGTCGCGGA AGTCGAGCTG AGGGCTGTGC CGGCGGGGAG CGGGCCAGGC
    AGATCGCTGC CGTGTGGGTC AGCAATTCAG GAGCAGGTGG ACTGGCGGAT CAAGCTTAGG
    GTCTCATGGT TTCACAGTCA AGATGTTACA GTTGCTGAAG GCTTGATGGA AATTGGGGAA
    CTTGCCTCCA CAGCGTCTCA CTTACATAGG GAAGAGTGTG ATCTGTGTGC CAGTGCCAGC
    GCAGCTATGA GTTGCTCTCG ATCACAGTTG ATTGCTATTA TAATTGACCT GGGTTCTGGA
    AAAGGCTACC TAAGCTCCTT TTTGTCCTTA AAGTATGGCT TAAAAGTTTA TGGAATCGAT
    TCCTCAAATA CTCGTGGAGC TGAGGAGAGA AACAGGAAAC TGAAGAAACA TTGGAAAGTC
    TATGGTAGGC GGTCAAAACC AGATGTCAGT GGACTGGCAT TACAGACAAC GAAAGAAAGG
    AAAGTGCAAG ATGAAACTAA AGCAGACATT GAGGGAATAT GTAACAGCAG TACTGCAAGT
    CAAGAGAAGC CACCAGCCTC AGACTTTTTA TCAGAGTTTT CAGGTTCTGT GATTTCTGAC
    ATCAGAAGAC AAATGGAAAA CCTAAAGGTT TATTCACACC GAGAGGAAAA TTTGTGTTTT
    GAAAATGCCT TTTCTCTTAG AGACCTTTTG CCTGTTAATG CCATAGAACC TAATTCCTCA
    TCCCAAATAC CTAAGAGAAA AATGTCTGAA GCAAGTAAAG AGAGAAGAAA AATTACTTTG
    AAGTCAAATG AATCAAATAT ATATTCACCT TTAACATCTA TTATCACTGC TGATTCAGAA
    CTACATGATA TTATTAAAGA TCTGGAGGAT TGTTTGATGG TAGGTCTACA TACTTGTGGT
    GATCTTGCTC CAAATACTCT GCGAATATTC ACTTCCAAGT CTGAAGTCAA AGGAGTTTGC
    AGTGTGGGTT GCTGCTATCA CCTCTTATCT GAAGAGTTTG AAAACCTACA TAAAGAATAT
    ACCCAAGAAA GGCGGGGATT TCCAATGTGC CACTATTTGA AGGAAGAGAG ATGGTGCTGT
    GGCCGAAACG CCAGAATGTC AGCCTGTTTG GCTCTGGAGC GGGTTGCAGT TGGCCAAGGG
    CTGCCTACTG AGTCCCTCTT CTATCGTGCC ATACTCCAGG ATATTATTAA AGAGTGCTAT
    GGCATCACCA AATGTGACCG GCATGTTGGT AAAATTTATT CCAAATCCTC CTCCTTTCCG
    GATTATGTCA GAAAGTCTCT AAAGAAGCTT GGGTTGGATG AGTCCAAGCT GCCAGAAAAA
    ATTATAATGG ACTACTACGA GAAATATAAG CCTAGAATGA ATGAACTGGA GGCTTTTAAT
    ATGTTGAAAG TTGTTCTAGC TCCTTGCATA GAGACCTTGA TTCTTTTGGA TCGACTTTGC
    TACCTGAAAG AGCAGGATGA TATCATGTGG TCTGCGCTTG TGAGGTTGTT TGATCCTGTG
    AAATCTCCCA GATGTTATGC CATTATTGCC CTGAAGAAGC AGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04172
    Clone ID Related Accession (Same CDS sequence) XM_024992401.1 , XM_024992400.1
    Accession Version XM_024992401.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyltransferase-like protein 25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGATGTTA CTGATTTTCC AAAAATATTT TGTGAAACTT CCCAGAAGCT GGTGAATGTG
    GAAGCATTTG CTCTGGCTGC AAAGTATTAT TCTGTACAAA ACTTGGGGAT ATGTACTTCT
    TTTGATCAAC TTCTTGTGGC CCTTCATGGA AATCAAAAAC AGAAAACTGA TGAGAATGTG
    AAACTTGATG AGTTTATGAA TTTGAAAAAA TCTCACGAAG TTCAGGCAAT GTCAGAACTC
    ATCAGCAGTA TTGCTGAATA CTGTGGAATA AAGCAGATAA TTGACCTGGG TTCTGGAAAA
    GGCTACCTAA GCTCCTTTTT GTCCTTAAAG TATGGCTTAA AAGTTTATGG AATCGATTCC
    TCAAATACTC GTGGAGCTGA GGAGAGAAAC AGGAAACTGA AGAAACATTG GAAAGTCTAT
    GGTAGGCGGT CAAAACCAGA TGTCAGTGGA CTGGCATTAC AGACAACGAA AGAAAGGAAA
    GTGCAAGATG AAACTAAAGC AGACATTGAG GGAATATGTA ACAGCAGTAC TGCAAGTCAA
    GAGAAGCCAC CAGCCTCAGA CTTTTTATCA GAGTTTTCAG GTTCTGTGAT TTCTGACATC
    AGAAGACAAA TGGAAAACCT AAAGGTTTAT TCACACCGAG AGGAAAATTT GTGTTTTGAA
    AATGCCTTTT CTCTTAGAGA CCTTTTGCCT GTTAATGCCA TAGAACCTAA TTCCTCATCC
    CAAATACCTA AGAGAAAAAT GTCTGAAGCA AGTAAAGAGA GAAGAAAAAT TACTTTGAAG
    TCAAATGAAT CAAATATATA TTCACCTTTA ACATCTATTA TCACTGCTGA TTCAGAACTA
    CATGATATTA TTAAAGATCT GGAGGATTGT TTGATGGTAG GTCTACATAC TTGTGGTGAT
    CTTGCTCCAA ATACTCTGCG AATATTCACT TCCAAGTCTG AAGTCAAAGG AGTTTGCAGT
    GTGGGTTGCT GCTATCACCT CTTATCTGAA GAGTTTGAAA ACCTACATAA AGAATATACC
    CAAGAAAGGC GGGGATTTCC AATGTGCCAC TATTTGAAGG AAGAGAGATG GTGCTGTGGC
    CGAAACGCCA GAATGTCAGC CTGTTTGGCT CTGGAGCGGG TTGCAGTTGG CCAAGGGCTG
    CCTACTGAGT CCCTCTTCTA TCGTGCCATA CTCCAGGATA TTATTAAAGA GTGCTATGGC
    ATCACCAAAT GTGACCGGCA TGTTGGTAAA ATTTATTCCA AATCCTCCTC CTTTCCGGAT
    TATGTCAGAA AGTCTCTAAA GAAGCTTGGG TTGGATGAGT CCAAGCTGCC AGAAAAAATT
    ATAATGGACT ACTACGAGAA ATATAAGCCT AGAATGAATG AACTGGAGGC TTTTAATATG
    TTGAAAGTTG TTCTAGCTCC TTGCATAGAG ACCTTGATTC TTTTGGATCG ACTTTGCTAC
    CTGAAAGAGC AGGATGATAT CATGTGGTCT GCGCTTGTGA GGTTGTTTGA TCCTGTGAAA
    TCTCCCAGAT GTTATGCCAT TATTGCCCTG AAGAAGCAGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848169.1
    CDS31..1815
    Translation

    Target ORF information:

    RefSeq Version XM_024992401.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyltransferase like 25 (METTL25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992401.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGATGTTA CTGATTTTCC AAAAATATTT TGTGAAACTT CCCAGAAGCT GGTGAATGTG
    GAAGCATTTG CTCTGGCTGC AAAGTATTAT TCTGTACAAA ACTTGGGGAT ATGTACTTCT
    TTTGATCAAC TTCTTGTGGC CCTTCATGGA AATCAAAAAC AGAAAACTGA TGAGAATGTG
    AAACTTGATG AGTTTATGAA TTTGAAAAAA TCTCACGAAG TTCAGGCAAT GTCAGAACTC
    ATCAGCAGTA TTGCTGAATA CTGTGGAATA AAGCAGATAA TTGACCTGGG TTCTGGAAAA
    GGCTACCTAA GCTCCTTTTT GTCCTTAAAG TATGGCTTAA AAGTTTATGG AATCGATTCC
    TCAAATACTC GTGGAGCTGA GGAGAGAAAC AGGAAACTGA AGAAACATTG GAAAGTCTAT
    GGTAGGCGGT CAAAACCAGA TGTCAGTGGA CTGGCATTAC AGACAACGAA AGAAAGGAAA
    GTGCAAGATG AAACTAAAGC AGACATTGAG GGAATATGTA ACAGCAGTAC TGCAAGTCAA
    GAGAAGCCAC CAGCCTCAGA CTTTTTATCA GAGTTTTCAG GTTCTGTGAT TTCTGACATC
    AGAAGACAAA TGGAAAACCT AAAGGTTTAT TCACACCGAG AGGAAAATTT GTGTTTTGAA
    AATGCCTTTT CTCTTAGAGA CCTTTTGCCT GTTAATGCCA TAGAACCTAA TTCCTCATCC
    CAAATACCTA AGAGAAAAAT GTCTGAAGCA AGTAAAGAGA GAAGAAAAAT TACTTTGAAG
    TCAAATGAAT CAAATATATA TTCACCTTTA ACATCTATTA TCACTGCTGA TTCAGAACTA
    CATGATATTA TTAAAGATCT GGAGGATTGT TTGATGGTAG GTCTACATAC TTGTGGTGAT
    CTTGCTCCAA ATACTCTGCG AATATTCACT TCCAAGTCTG AAGTCAAAGG AGTTTGCAGT
    GTGGGTTGCT GCTATCACCT CTTATCTGAA GAGTTTGAAA ACCTACATAA AGAATATACC
    CAAGAAAGGC GGGGATTTCC AATGTGCCAC TATTTGAAGG AAGAGAGATG GTGCTGTGGC
    CGAAACGCCA GAATGTCAGC CTGTTTGGCT CTGGAGCGGG TTGCAGTTGG CCAAGGGCTG
    CCTACTGAGT CCCTCTTCTA TCGTGCCATA CTCCAGGATA TTATTAAAGA GTGCTATGGC
    ATCACCAAAT GTGACCGGCA TGTTGGTAAA ATTTATTCCA AATCCTCCTC CTTTCCGGAT
    TATGTCAGAA AGTCTCTAAA GAAGCTTGGG TTGGATGAGT CCAAGCTGCC AGAAAAAATT
    ATAATGGACT ACTACGAGAA ATATAAGCCT AGAATGAATG AACTGGAGGC TTTTAATATG
    TTGAAAGTTG TTCTAGCTCC TTGCATAGAG ACCTTGATTC TTTTGGATCG ACTTTGCTAC
    CTGAAAGAGC AGGATGATAT CATGTGGTCT GCGCTTGTGA GGTTGTTTGA TCCTGTGAAA
    TCTCCCAGAT GTTATGCCAT TATTGCCCTG AAGAAGCAGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04172
    Clone ID Related Accession (Same CDS sequence) XM_024992401.1 , XM_024992400.1
    Accession Version XM_024992400.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyltransferase-like protein 25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGATGTTA CTGATTTTCC AAAAATATTT TGTGAAACTT CCCAGAAGCT GGTGAATGTG
    GAAGCATTTG CTCTGGCTGC AAAGTATTAT TCTGTACAAA ACTTGGGGAT ATGTACTTCT
    TTTGATCAAC TTCTTGTGGC CCTTCATGGA AATCAAAAAC AGAAAACTGA TGAGAATGTG
    AAACTTGATG AGTTTATGAA TTTGAAAAAA TCTCACGAAG TTCAGGCAAT GTCAGAACTC
    ATCAGCAGTA TTGCTGAATA CTGTGGAATA AAGCAGATAA TTGACCTGGG TTCTGGAAAA
    GGCTACCTAA GCTCCTTTTT GTCCTTAAAG TATGGCTTAA AAGTTTATGG AATCGATTCC
    TCAAATACTC GTGGAGCTGA GGAGAGAAAC AGGAAACTGA AGAAACATTG GAAAGTCTAT
    GGTAGGCGGT CAAAACCAGA TGTCAGTGGA CTGGCATTAC AGACAACGAA AGAAAGGAAA
    GTGCAAGATG AAACTAAAGC AGACATTGAG GGAATATGTA ACAGCAGTAC TGCAAGTCAA
    GAGAAGCCAC CAGCCTCAGA CTTTTTATCA GAGTTTTCAG GTTCTGTGAT TTCTGACATC
    AGAAGACAAA TGGAAAACCT AAAGGTTTAT TCACACCGAG AGGAAAATTT GTGTTTTGAA
    AATGCCTTTT CTCTTAGAGA CCTTTTGCCT GTTAATGCCA TAGAACCTAA TTCCTCATCC
    CAAATACCTA AGAGAAAAAT GTCTGAAGCA AGTAAAGAGA GAAGAAAAAT TACTTTGAAG
    TCAAATGAAT CAAATATATA TTCACCTTTA ACATCTATTA TCACTGCTGA TTCAGAACTA
    CATGATATTA TTAAAGATCT GGAGGATTGT TTGATGGTAG GTCTACATAC TTGTGGTGAT
    CTTGCTCCAA ATACTCTGCG AATATTCACT TCCAAGTCTG AAGTCAAAGG AGTTTGCAGT
    GTGGGTTGCT GCTATCACCT CTTATCTGAA GAGTTTGAAA ACCTACATAA AGAATATACC
    CAAGAAAGGC GGGGATTTCC AATGTGCCAC TATTTGAAGG AAGAGAGATG GTGCTGTGGC
    CGAAACGCCA GAATGTCAGC CTGTTTGGCT CTGGAGCGGG TTGCAGTTGG CCAAGGGCTG
    CCTACTGAGT CCCTCTTCTA TCGTGCCATA CTCCAGGATA TTATTAAAGA GTGCTATGGC
    ATCACCAAAT GTGACCGGCA TGTTGGTAAA ATTTATTCCA AATCCTCCTC CTTTCCGGAT
    TATGTCAGAA AGTCTCTAAA GAAGCTTGGG TTGGATGAGT CCAAGCTGCC AGAAAAAATT
    ATAATGGACT ACTACGAGAA ATATAAGCCT AGAATGAATG AACTGGAGGC TTTTAATATG
    TTGAAAGTTG TTCTAGCTCC TTGCATAGAG ACCTTGATTC TTTTGGATCG ACTTTGCTAC
    CTGAAAGAGC AGGATGATAT CATGTGGTCT GCGCTTGTGA GGTTGTTTGA TCCTGTGAAA
    TCTCCCAGAT GTTATGCCAT TATTGCCCTG AAGAAGCAGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848168.1
    CDS31..1815
    Translation

    Target ORF information:

    RefSeq Version XM_024992400.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyltransferase like 25 (METTL25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGATGTTA CTGATTTTCC AAAAATATTT TGTGAAACTT CCCAGAAGCT GGTGAATGTG
    GAAGCATTTG CTCTGGCTGC AAAGTATTAT TCTGTACAAA ACTTGGGGAT ATGTACTTCT
    TTTGATCAAC TTCTTGTGGC CCTTCATGGA AATCAAAAAC AGAAAACTGA TGAGAATGTG
    AAACTTGATG AGTTTATGAA TTTGAAAAAA TCTCACGAAG TTCAGGCAAT GTCAGAACTC
    ATCAGCAGTA TTGCTGAATA CTGTGGAATA AAGCAGATAA TTGACCTGGG TTCTGGAAAA
    GGCTACCTAA GCTCCTTTTT GTCCTTAAAG TATGGCTTAA AAGTTTATGG AATCGATTCC
    TCAAATACTC GTGGAGCTGA GGAGAGAAAC AGGAAACTGA AGAAACATTG GAAAGTCTAT
    GGTAGGCGGT CAAAACCAGA TGTCAGTGGA CTGGCATTAC AGACAACGAA AGAAAGGAAA
    GTGCAAGATG AAACTAAAGC AGACATTGAG GGAATATGTA ACAGCAGTAC TGCAAGTCAA
    GAGAAGCCAC CAGCCTCAGA CTTTTTATCA GAGTTTTCAG GTTCTGTGAT TTCTGACATC
    AGAAGACAAA TGGAAAACCT AAAGGTTTAT TCACACCGAG AGGAAAATTT GTGTTTTGAA
    AATGCCTTTT CTCTTAGAGA CCTTTTGCCT GTTAATGCCA TAGAACCTAA TTCCTCATCC
    CAAATACCTA AGAGAAAAAT GTCTGAAGCA AGTAAAGAGA GAAGAAAAAT TACTTTGAAG
    TCAAATGAAT CAAATATATA TTCACCTTTA ACATCTATTA TCACTGCTGA TTCAGAACTA
    CATGATATTA TTAAAGATCT GGAGGATTGT TTGATGGTAG GTCTACATAC TTGTGGTGAT
    CTTGCTCCAA ATACTCTGCG AATATTCACT TCCAAGTCTG AAGTCAAAGG AGTTTGCAGT
    GTGGGTTGCT GCTATCACCT CTTATCTGAA GAGTTTGAAA ACCTACATAA AGAATATACC
    CAAGAAAGGC GGGGATTTCC AATGTGCCAC TATTTGAAGG AAGAGAGATG GTGCTGTGGC
    CGAAACGCCA GAATGTCAGC CTGTTTGGCT CTGGAGCGGG TTGCAGTTGG CCAAGGGCTG
    CCTACTGAGT CCCTCTTCTA TCGTGCCATA CTCCAGGATA TTATTAAAGA GTGCTATGGC
    ATCACCAAAT GTGACCGGCA TGTTGGTAAA ATTTATTCCA AATCCTCCTC CTTTCCGGAT
    TATGTCAGAA AGTCTCTAAA GAAGCTTGGG TTGGATGAGT CCAAGCTGCC AGAAAAAATT
    ATAATGGACT ACTACGAGAA ATATAAGCCT AGAATGAATG AACTGGAGGC TTTTAATATG
    TTGAAAGTTG TTCTAGCTCC TTGCATAGAG ACCTTGATTC TTTTGGATCG ACTTTGCTAC
    CTGAAAGAGC AGGATGATAT CATGTGGTCT GCGCTTGTGA GGTTGTTTGA TCCTGTGAAA
    TCTCCCAGAT GTTATGCCAT TATTGCCCTG AAGAAGCAGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89847
    Clone ID Related Accession (Same CDS sequence) XM_015463258.1
    Accession Version XM_015463258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: methyltransferase-like protein 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644769.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGGTGAAT GGCGGGTGGA TGTTACTGAT TTTCCAAAAN NATTTTGTGA AACTTCCCAG
    AAGCTGGTGA ATGTGGAAGC ATTTGCTCTG GCTGCAAAGT ATTATTCTGT ACAAAACTTG
    GGGATATGTA CTTCTTTTGA TCAACTTCTT GTGGCCCTTC ATGGAAATCA AAAACAGAAA
    ACTGATGAGA ATGTGAAACT TGATGAGTTT ATGAATTTGA AAAAATCTCA CGAAGTTCAG
    GCAATGTCAG AACTCATCAG CAGTATTGCT GAATACTGTG GAATAAAGCA GATAATTGAC
    CTGGGTTCTG GAAAAGGCTA CCTAAGCTCC TTTTTGTCCT TAAAGTATGG CTTAAAAGTT
    TATGGAATCG ATTCCTCAAA TACTCGTGGA GCTGAGGAGA GAAACAGGAA ACTGAAGAAA
    CATTGGAAAG TCTATGGTAG GCGGTCAAAA CCAGATGTCA GTGGACTGGC ATTACAGACA
    ACGAAAGAAA GGAAAGTGCA AGATGAAACT AAAGCAGACA TTGAGGGAAT ATGTAACAGC
    AGTACTGCAA GTCAAGAGAA GCCACCAGCC TCAGACTTTT TATCAGAGTT TTCAGGTTCT
    GTGATTTCTG ACATCAGAAG ACAAATGGAA AACCTAAAGG TTTATTCACA CCGAGAGGAA
    AATTTGTGTT TTGAAAATGC CTTTTCTCTT AGAGACCTTT TGCCTGTTAA TGCCATAGAA
    CCTAATTCCT CATCCCAAAT ACCTAAGAGA AAAATGTCTG AAGCAAGTAA AGAGAGAAGA
    AAAATTACTT TGAAGTCAAA TGAATCAAAT ATATATTCAC CTTTAACATC TATTATCACT
    GCTGATTCAG AACTACATGA TATTATTAAA GATCTGGAGG ATTGTTTGAT GGTAGGTCTA
    CATACTTGTG GTGATCTTGC TCCAAATACT CTGCGAATAT TCACTTCCAA GTCTGAAGTC
    AAAGGAGTTT GCAGTGTGGG TTGCTGCTAT CACCTCTTAT CTGAAGAGTT TGAAAACCTA
    CATAAAGAAT ATACCCAAGA AAGGCGGGGA TTTCCAATGT GCCACTATTT GAAGGAAGAG
    AGATGGTGCT GTGGCCGAAA CGCCAGAATG TCAGCCTGTT TGGCTCTGGA GCGGGTTGCA
    GTTGGCCAAG GGCTGCCTAC TGAGTCCCTC TTCTATCGTG CCATACTCCA GGATATTATT
    AAAGAGTGCT ATGGCATCAC CAAATGTGAC CGGCATGTTG GTAAAATTTA TTCCAAATCC
    TCCTCCTTTC CGGATTATGT CAGAAAGTCT CTAAAGAAGC TTGGGTTGGA TGAGTCCAAG
    CTGCCAGAAA AAATTATAAT GGACTACTAC GAGAAATATA AGCCTAGAAT GAATGAACTG
    GAGGCTTTTA ATATGTTGAA AGTTGTTCTA GCTCCTTGCA TAGAGACCTT GATTCTTTTG
    GATCGACTTT GCTACCTGAA AGAGCAGGAT GATATCATGT GGTCTGCGCT TGTGAGGTTG
    TTTGATCCTG TGAAATCTCC CAGATGTTAT GCCATTATTG CCCTGAAGAA GCAGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318744.1
    CDS1..1800
    Translation

    Target ORF information:

    RefSeq Version XM_015463258.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyltransferase like 25 (METTL25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCTT CCGGCCAGCT CCCGGTGACC CAGGACCTGT CCACGTTACA TGCCAAGTTG 
    CAGAAGCTGC TGCGGTTCCT GAGGGAAGCC CTGCCCATTT CGAATGCACA TACGGTGGAC
    TTCTACACGG AGTCTGTGTG GGAGAAACTG GTTGACTTGC CCCCAGAGAC GGTGCTGTCG
    GCGCTCAGGA CGTCCGCGGC GGCGTCAGAG GCGTGCCCCC TAGAGGAAGC CGGGAGAATG
    TCAGGTGAAT GGCGGGTGGA TGTTACTGAT TTTCCAAAAN NATTTTGTGA AACTTCCCAG
    AAGCTGGTGA ATGTGGAAGC ATTTGCTCTG GCTGCAAAGT ATTATTCTGT ACAAAACTTG
    GGGATATGTA CTTCTTTTGA TCAACTTCTT GTGGCCCTTC ATGGAAATCA AAAACAGAAA
    ACTGATGAGA ATGTGAAACT TGATGAGTTT ATGAATTTGA AAAAATCTCA CGAAGTTCAG
    GCAATGTCAG AACTCATCAG CAGTATTGCT GAATACTGTG GAATAAAGCA GATAATTGAC
    CTGGGTTCTG GAAAAGGCTA CCTAAGCTCC TTTTTGTCCT TAAAGTATGG CTTAAAAGTT
    TATGGAATCG ATTCCTCAAA TACTCGTGGA GCTGAGGAGA GAAACAGGAA ACTGAAGAAA
    CATTGGAAAG TCTATGGTAG GCGGTCAAAA CCAGATGTCA GTGGACTGGC ATTACAGACA
    ACGAAAGAAA GGAAAGTGCA AGATGAAACT AAAGCAGACA TTGAGGGAAT ATGTAACAGC
    AGTACTGCAA GTCAAGAGAA GCCACCAGCC TCAGACTTTT TATCAGAGTT TTCAGGTTCT
    GTGATTTCTG ACATCAGAAG ACAAATGGAA AACCTAAAGG TTTATTCACA CCGAGAGGAA
    AATTTGTGTT TTGAAAATGC CTTTTCTCTT AGAGACCTTT TGCCTGTTAA TGCCATAGAA
    CCTAATTCCT CATCCCAAAT ACCTAAGAGA AAAATGTCTG AAGCAAGTAA AGAGAGAAGA
    AAAATTACTT TGAAGTCAAA TGAATCAAAT ATATATTCAC CTTTAACATC TATTATCACT
    GCTGATTCAG AACTACATGA TATTATTAAA GATCTGGAGG ATTGTTTGAT GGTAGGTCTA
    CATACTTGTG GTGATCTTGC TCCAAATACT CTGCGAATAT TCACTTCCAA GTCTGAAGTC
    AAAGGAGTTT GCAGTGTGGG TTGCTGCTAT CACCTCTTAT CTGAAGAGTT TGAAAACCTA
    CATAAAGAAT ATACCCAAGA AAGGCGGGGA TTTCCAATGT GCCACTATTT GAAGGAAGAG
    AGATGGTGCT GTGGCCGAAA CGCCAGAATG TCAGCCTGTT TGGCTCTGGA GCGGGTTGCA
    GTTGGCCAAG GGCTGCCTAC TGAGTCCCTC TTCTATCGTG CCATACTCCA GGATATTATT
    AAAGAGTGCT ATGGCATCAC CAAATGTGAC CGGCATGTTG GTAAAATTTA TTCCAAATCC
    TCCTCCTTTC CGGATTATGT CAGAAAGTCT CTAAAGAAGC TTGGGTTGGA TGAGTCCAAG
    CTGCCAGAAA AAATTATAAT GGACTACTAC GAGAAATATA AGCCTAGAAT GAATGAACTG
    GAGGCTTTTA ATATGTTGAA AGTTGTTCTA GCTCCTTGCA TAGAGACCTT GATTCTTTTG
    GATCGACTTT GCTACCTGAA AGAGCAGGAT GATATCATGT GGTCTGCGCT TGTGAGGTTG
    TTTGATCCTG TGAAATCTCC CAGATGTTAT GCCATTATTG CCCTGAAGAA GCAGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.