HCFC1 cDNA ORF clone, Bos taurus(cattle)

The following HCFC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCFC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238740 XM_024988693.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa12917 XM_024988692.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa12923 XM_024988696.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa48469 XM_024988687.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48470 XM_024988688.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48471 XM_024988689.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48472 XM_024988690.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48473 XM_015461524.2
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48474 XM_024988691.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48475 XM_024988694.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa48476 XM_024988695.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa89280 XM_015461524.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa89280 XM_010800388.2
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa89281 XM_015470290.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa89281 XM_015461525.1
Latest version!
Bos taurus host cell factor C1 (HCFC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238740
    Clone ID Related Accession (Same CDS sequence) XM_024988693.1
    Accession Version XM_024988693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCCACCCAG
    TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAAGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATACA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGGCACC GGGGAGCCCA TGGATACTTC AGAGGCGGCC
    GCAGCGGTGA CACAGGCAGA GTTGAGCCAC CTGTCGGCCG AGGGCCAGGA AGGCCAGGCG
    ACCACTATCC CCATCGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG
    CTCCAGGAAG CACAGGCCCA GCAGCAGCAG CACCACCTGC CCACGGAAGC ACTGGCCCCG
    GCTGACAGCC TCAATGACCC GACCATCGAG AGCAACTGCC TCAATGAGCT GGCCGGGGCT
    GTGCCCAGCA CCGTGAGCCT GCTGCCCCCC ACGGCCACTG AGAGCCTGGC CCCGTCCAAC
    ACATTTGTGG CCCCCCAGCC GGTCGTTGTG GCCAGCCCCG CGAAGCTGCA GGCCGCAGCC
    ACCCTGACGG AAGTGGCCAA TGGCATTGAG TCCCTGGGTG TGAAGCCAGA CCTGCCACCG
    CCACCTAGCA AAGCCCCTGT AAAGAAGGAG AACCAGTGGT TTGACGTGGG GGTCATTAAG
    GGTACCAATG TGATGGTGAC ACACTATTTC CTGCCACCCG AAGATGCTGT CCCGACTGAT
    GATGACTCGG GCACCGTGCC CGACTACAAC CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC
    ACTGCCTATA AGTTCCGCGT CGCCGGGATC AATGCCTGCG GCCGGGGGCC CTTCAGTGAG
    ATCTCAGCCT TTAAAACGTG TCTGCCTGGC TTCCCAGGGG CCCCGTGTGC CATTAAAATC
    AGCAAAAGTC CAGACGGTGC TCACCTCACC TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG
    ATCATCGAGT ACTCAGTGTA CCTGGCCATC CAGAGCGCGC AGGCCGGTGG TGAGACCAAG
    AGCTCGACCC CGGCGCAGCT GGCCTTCATG CGGGTGTACT GCGGGCCCAG CCCCTCCTGC
    CTCGTGCAGT CCTCCAGCCT CTCCAACGCC CACATCGACT ACACCACCAA GCCCGCCATC
    ATCTTCCGCA TTGCTGCCCG CAATGAGAAG GGCTACGGCC CAGCCACCCA AGTCAGGTGG
    TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC GCCAAGCCGG CCAGCAAGCG GCCCATGTCG
    TCTCCAGAAA TGAAATCCGC GCCAAAGAAA TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844461.1
    CDS1052..7162
    Translation

    Target ORF information:

    RefSeq Version XM_024988693.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCCACCCAG
    TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAAGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATACA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGGCACC GGGGAGCCCA TGGATACTTC AGAGGCGGCC
    GCAGCGGTGA CACAGGCAGA GTTGAGCCAC CTGTCGGCCG AGGGCCAGGA AGGCCAGGCG
    ACCACTATCC CCATCGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG
    CTCCAGGAAG CACAGGCCCA GCAGCAGCAG CACCACCTGC CCACGGAAGC ACTGGCCCCG
    GCTGACAGCC TCAATGACCC GACCATCGAG AGCAACTGCC TCAATGAGCT GGCCGGGGCT
    GTGCCCAGCA CCGTGAGCCT GCTGCCCCCC ACGGCCACTG AGAGCCTGGC CCCGTCCAAC
    ACATTTGTGG CCCCCCAGCC GGTCGTTGTG GCCAGCCCCG CGAAGCTGCA GGCCGCAGCC
    ACCCTGACGG AAGTGGCCAA TGGCATTGAG TCCCTGGGTG TGAAGCCAGA CCTGCCACCG
    CCACCTAGCA AAGCCCCTGT AAAGAAGGAG AACCAGTGGT TTGACGTGGG GGTCATTAAG
    GGTACCAATG TGATGGTGAC ACACTATTTC CTGCCACCCG AAGATGCTGT CCCGACTGAT
    GATGACTCGG GCACCGTGCC CGACTACAAC CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC
    ACTGCCTATA AGTTCCGCGT CGCCGGGATC AATGCCTGCG GCCGGGGGCC CTTCAGTGAG
    ATCTCAGCCT TTAAAACGTG TCTGCCTGGC TTCCCAGGGG CCCCGTGTGC CATTAAAATC
    AGCAAAAGTC CAGACGGTGC TCACCTCACC TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG
    ATCATCGAGT ACTCAGTGTA CCTGGCCATC CAGAGCGCGC AGGCCGGTGG TGAGACCAAG
    AGCTCGACCC CGGCGCAGCT GGCCTTCATG CGGGTGTACT GCGGGCCCAG CCCCTCCTGC
    CTCGTGCAGT CCTCCAGCCT CTCCAACGCC CACATCGACT ACACCACCAA GCCCGCCATC
    ATCTTCCGCA TTGCTGCCCG CAATGAGAAG GGCTACGGCC CAGCCACCCA AGTCAGGTGG
    TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC GCCAAGCCGG CCAGCAAGCG GCCCATGTCG
    TCTCCAGAAA TGAAATCCGC GCCAAAGAAA TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12917
    Clone ID Related Accession (Same CDS sequence) XM_024988692.1
    Accession Version XM_024988692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCCACCCAG
    TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAAGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATACA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCAGCGG TGACACAGGC AGAGTTGAGC CACCTGTCGG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACACAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCACTGGCC
    CCGGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGAG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCCCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCA
    GCCACCCTGA CGGAAGTGGC CAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGATGC TGTCCCGACT
    GATGATGACT CGGGCACCGT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA
    GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GCGGCCGGGG GCCCTTCAGT
    GAGATCTCAG CCTTTAAAAC GTGTCTGCCT GGCTTCCCAG GGGCCCCGTG TGCCATTAAA
    ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CACCCTCTGT GACCTCCGGC
    AAGATCATCG AGTACTCAGT GTACCTGGCC ATCCAGAGCG CGCAGGCCGG TGGTGAGACC
    AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC
    TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATCG ACTACACCAC CAAGCCCGCC
    ATCATCTTCC GCATTGCTGC CCGCAATGAG AAGGGCTACG GCCCAGCCAC CCAAGTCAGG
    TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG
    TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844460.1
    CDS1053..7166
    Translation

    Target ORF information:

    RefSeq Version XM_024988692.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCCACCCAG
    TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAAGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATACA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCAGCGG TGACACAGGC AGAGTTGAGC CACCTGTCGG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACACAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCACTGGCC
    CCGGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGAG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCCCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCA
    GCCACCCTGA CGGAAGTGGC CAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGATGC TGTCCCGACT
    GATGATGACT CGGGCACCGT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA
    GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GCGGCCGGGG GCCCTTCAGT
    GAGATCTCAG CCTTTAAAAC GTGTCTGCCT GGCTTCCCAG GGGCCCCGTG TGCCATTAAA
    ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CACCCTCTGT GACCTCCGGC
    AAGATCATCG AGTACTCAGT GTACCTGGCC ATCCAGAGCG CGCAGGCCGG TGGTGAGACC
    AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC
    TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATCG ACTACACCAC CAAGCCCGCC
    ATCATCTTCC GCATTGCTGC CCGCAATGAG AAGGGCTACG GCCCAGCCAC CCAAGTCAGG
    TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG
    TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12923
    Clone ID Related Accession (Same CDS sequence) XM_024988696.1
    Accession Version XM_024988696.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG
    CTCCAGGCCT CTCCAGGGCC ACGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC
    TCCGCGCCCT TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG CCCTGAGCTC
    CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CAGGCCAGGA GCCTGCCAGC
    TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCGC CCACCCAGTC CGAAGTAGAC
    CAGTTGTCCC TCCCCCAAGA GCTGATGGCC GAAGCCCAGG CGGGCACCAC CACCCTCATG
    GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC GGCCGCGCAG
    GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATACAGG CCGTGCTCCA GGCCGCACAG
    CAGGCTGTCA TGGGCACCGG GGAGCCCATG GATACTTCAG AGGCGGCCGC AGCGGTGACA
    CAGGCAGAGT TGAGCCACCT GTCGGCCGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC
    ATCGTGCTGA CACAGCAGGA GCTGGCTGCC CTGGTCCAGC AGCAGCAGCT CCAGGAAGCA
    CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCAC TGGCCCCGGC TGACAGCCTC
    AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC
    GTGAGCCTGC TGCCCCCCAC GGCCACTGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC
    CCCCAGCCGG TCGTTGTGGC CAGCCCCGCG AAGCTGCAGG CCGCAGCCAC CCTGACGGAA
    GTGGCCAATG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA
    GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TCATTAAGGG TACCAATGTG
    ATGGTGACAC ACTATTTCCT GCCACCCGAA GATGCTGTCC CGACTGATGA TGACTCGGGC
    ACCGTGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG
    TTCCGCGTCG CCGGGATCAA TGCCTGCGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT
    AAAACGTGTC TGCCTGGCTT CCCAGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA
    GACGGTGCTC ACCTCACCTG GGAGCCACCC TCTGTGACCT CCGGCAAGAT CATCGAGTAC
    TCAGTGTACC TGGCCATCCA GAGCGCGCAG GCCGGTGGTG AGACCAAGAG CTCGACCCCG
    GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC
    TCCAGCCTCT CCAACGCCCA CATCGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATT
    GCTGCCCGCA ATGAGAAGGG CTACGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC
    AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG
    AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844464.1
    CDS1052..7030
    Translation

    Target ORF information:

    RefSeq Version XM_024988696.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988696.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG
    CTCCAGGCCT CTCCAGGGCC ACGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC
    TCCGCGCCCT TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG CCCTGAGCTC
    CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CAGGCCAGGA GCCTGCCAGC
    TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCGC CCACCCAGTC CGAAGTAGAC
    CAGTTGTCCC TCCCCCAAGA GCTGATGGCC GAAGCCCAGG CGGGCACCAC CACCCTCATG
    GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC GGCCGCGCAG
    GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATACAGG CCGTGCTCCA GGCCGCACAG
    CAGGCTGTCA TGGGCACCGG GGAGCCCATG GATACTTCAG AGGCGGCCGC AGCGGTGACA
    CAGGCAGAGT TGAGCCACCT GTCGGCCGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC
    ATCGTGCTGA CACAGCAGGA GCTGGCTGCC CTGGTCCAGC AGCAGCAGCT CCAGGAAGCA
    CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCAC TGGCCCCGGC TGACAGCCTC
    AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC
    GTGAGCCTGC TGCCCCCCAC GGCCACTGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC
    CCCCAGCCGG TCGTTGTGGC CAGCCCCGCG AAGCTGCAGG CCGCAGCCAC CCTGACGGAA
    GTGGCCAATG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA
    GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TCATTAAGGG TACCAATGTG
    ATGGTGACAC ACTATTTCCT GCCACCCGAA GATGCTGTCC CGACTGATGA TGACTCGGGC
    ACCGTGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG
    TTCCGCGTCG CCGGGATCAA TGCCTGCGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT
    AAAACGTGTC TGCCTGGCTT CCCAGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA
    GACGGTGCTC ACCTCACCTG GGAGCCACCC TCTGTGACCT CCGGCAAGAT CATCGAGTAC
    TCAGTGTACC TGGCCATCCA GAGCGCGCAG GCCGGTGGTG AGACCAAGAG CTCGACCCCG
    GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC
    TCCAGCCTCT CCAACGCCCA CATCGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATT
    GCTGCCCGCA ATGAGAAGGG CTACGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC
    AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG
    AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48469
    Clone ID Related Accession (Same CDS sequence) XM_024988687.1
    Accession Version XM_024988687.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CAGCGGTGAC ACAGGCAGAG
    TTGAGCCACC TGTCGGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACACAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCA CTGGCCCCGG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGAGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCAGCCA CCCTGACGGA AGTGGCCAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGATGCTGTC CCGACTGATG TATGTATCCC TGGGAGCCAA
    CACCCTCAGG ATGACTCGGG CACCGTGCCC GACTACAACC AGCTAAAGAA GCAGGAGCTG
    CAGCCAGGCA CTGCCTATAA GTTCCGCGTC GCCGGGATCA ATGCCTGCGG CCGGGGGCCC
    TTCAGTGAGA TCTCAGCCTT TAAAACGTGT CTGCCTGGCT TCCCAGGGGC CCCGTGTGCC
    ATTAAAATCA GCAAAAGTCC AGACGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC
    TCCGGCAAGA TCATCGAGTA CTCAGTGTAC CTGGCCATCC AGAGCGCGCA GGCCGGTGGT
    GAGACCAAGA GCTCGACCCC GGCGCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC
    CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC TCCAACGCCC ACATCGACTA CACCACCAAG
    CCCGCCATCA TCTTCCGCAT TGCTGCCCGC AATGAGAAGG GCTACGGCCC AGCCACCCAA
    GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG
    CCCATGTCGT CTCCAGAAAT GAAATCCGCG CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844455.1
    CDS1054..7233
    Translation

    Target ORF information:

    RefSeq Version XM_024988687.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988687.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CAGCGGTGAC ACAGGCAGAG
    TTGAGCCACC TGTCGGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACACAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCA CTGGCCCCGG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGAGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCAGCCA CCCTGACGGA AGTGGCCAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGATGCTGTC CCGACTGATG TATGTATCCC TGGGAGCCAA
    CACCCTCAGG ATGACTCGGG CACCGTGCCC GACTACAACC AGCTAAAGAA GCAGGAGCTG
    CAGCCAGGCA CTGCCTATAA GTTCCGCGTC GCCGGGATCA ATGCCTGCGG CCGGGGGCCC
    TTCAGTGAGA TCTCAGCCTT TAAAACGTGT CTGCCTGGCT TCCCAGGGGC CCCGTGTGCC
    ATTAAAATCA GCAAAAGTCC AGACGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC
    TCCGGCAAGA TCATCGAGTA CTCAGTGTAC CTGGCCATCC AGAGCGCGCA GGCCGGTGGT
    GAGACCAAGA GCTCGACCCC GGCGCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC
    CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC TCCAACGCCC ACATCGACTA CACCACCAAG
    CCCGCCATCA TCTTCCGCAT TGCTGCCCGC AATGAGAAGG GCTACGGCCC AGCCACCCAA
    GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG
    CCCATGTCGT CTCCAGAAAT GAAATCCGCG CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48470
    Clone ID Related Accession (Same CDS sequence) XM_024988688.1
    Accession Version XM_024988688.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCAG CGGTGACACA GGCAGAGTTG
    AGCCACCTGT CGGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACA
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCACTG GCCCCGGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GAGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCAGCCACCC TGACGGAAGT GGCCAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA TGCTGTCCCG ACTGATGTAT GTATCCCTGG GAGCCAACAC
    CCTCAGGATG ACTCGGGCAC CGTGCCCGAC TACAACCAGC TAAAGAAGCA GGAGCTGCAG
    CCAGGCACTG CCTATAAGTT CCGCGTCGCC GGGATCAATG CCTGCGGCCG GGGGCCCTTC
    AGTGAGATCT CAGCCTTTAA AACGTGTCTG CCTGGCTTCC CAGGGGCCCC GTGTGCCATT
    AAAATCAGCA AAAGTCCAGA CGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC
    GGCAAGATCA TCGAGTACTC AGTGTACCTG GCCATCCAGA GCGCGCAGGC CGGTGGTGAG
    ACCAAGAGCT CGACCCCGGC GCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC
    TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC AACGCCCACA TCGACTACAC CACCAAGCCC
    GCCATCATCT TCCGCATTGC TGCCCGCAAT GAGAAGGGCT ACGGCCCAGC CACCCAAGTC
    AGGTGGTTGC AAGAAACCAG TAAAGACAGC TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC
    ATGTCGTCTC CAGAAATGAA ATCCGCGCCA AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844456.1
    CDS1054..7230
    Translation

    Target ORF information:

    RefSeq Version XM_024988688.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988688.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCAG CGGTGACACA GGCAGAGTTG
    AGCCACCTGT CGGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACA
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCACTG GCCCCGGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GAGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCAGCCACCC TGACGGAAGT GGCCAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA TGCTGTCCCG ACTGATGTAT GTATCCCTGG GAGCCAACAC
    CCTCAGGATG ACTCGGGCAC CGTGCCCGAC TACAACCAGC TAAAGAAGCA GGAGCTGCAG
    CCAGGCACTG CCTATAAGTT CCGCGTCGCC GGGATCAATG CCTGCGGCCG GGGGCCCTTC
    AGTGAGATCT CAGCCTTTAA AACGTGTCTG CCTGGCTTCC CAGGGGCCCC GTGTGCCATT
    AAAATCAGCA AAAGTCCAGA CGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC
    GGCAAGATCA TCGAGTACTC AGTGTACCTG GCCATCCAGA GCGCGCAGGC CGGTGGTGAG
    ACCAAGAGCT CGACCCCGGC GCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC
    TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC AACGCCCACA TCGACTACAC CACCAAGCCC
    GCCATCATCT TCCGCATTGC TGCCCGCAAT GAGAAGGGCT ACGGCCCAGC CACCCAAGTC
    AGGTGGTTGC AAGAAACCAG TAAAGACAGC TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC
    ATGTCGTCTC CAGAAATGAA ATCCGCGCCA AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48471
    Clone ID Related Accession (Same CDS sequence) XM_024988689.1
    Accession Version XM_024988689.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG
    ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT
    GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA
    GGGCCACGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCTTGGTG
    GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGCCCTG AGCTCCAGGC CGCCGTGGAT
    CTGAGCAGTA CAGGGGACCC GTCTTCAGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC
    ACCGTGGTGG TCCAGCCACC GCCGCCCACC CAGTCCGAAG TAGACCAGTT GTCCCTCCCC
    CAAGAGCTGA TGGCCGAAGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC
    CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCGGCCG CGCAGGCTGC AGCCACAGAG
    GAGGCCCAGG CCCTGGCCAT ACAGGCCGTG CTCCAGGCCG CACAGCAGGC TGTCATGGCA
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCAG CGGTGACACA GGCAGAGTTG
    AGCCACCTGT CGGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACA
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCACTG GCCCCGGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GAGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCAGCCACCC TGACGGAAGT GGCCAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA TGCTGTCCCG ACTGATGTAT GTATCCCTGG GAGCCAACAC
    CCTCAGGATG ACTCGGGCAC CGTGCCCGAC TACAACCAGC TAAAGAAGCA GGAGCTGCAG
    CCAGGCACTG CCTATAAGTT CCGCGTCGCC GGGATCAATG CCTGCGGCCG GGGGCCCTTC
    AGTGAGATCT CAGCCTTTAA AACGTGTCTG CCTGGCTTCC CAGGGGCCCC GTGTGCCATT
    AAAATCAGCA AAAGTCCAGA CGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC
    GGCAAGATCA TCGAGTACTC AGTGTACCTG GCCATCCAGA GCGCGCAGGC CGGTGGTGAG
    ACCAAGAGCT CGACCCCGGC GCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC
    TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC AACGCCCACA TCGACTACAC CACCAAGCCC
    GCCATCATCT TCCGCATTGC TGCCCGCAAT GAGAAGGGCT ACGGCCCAGC CACCCAAGTC
    AGGTGGTTGC AAGAAACCAG TAAAGACAGC TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC
    ATGTCGTCTC CAGAAATGAA ATCCGCGCCA AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844457.1
    CDS1054..7230
    Translation

    Target ORF information:

    RefSeq Version XM_024988689.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG
    ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT
    GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA
    GGGCCACGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCTTGGTG
    GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGCCCTG AGCTCCAGGC CGCCGTGGAT
    CTGAGCAGTA CAGGGGACCC GTCTTCAGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC
    ACCGTGGTGG TCCAGCCACC GCCGCCCACC CAGTCCGAAG TAGACCAGTT GTCCCTCCCC
    CAAGAGCTGA TGGCCGAAGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC
    CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCGGCCG CGCAGGCTGC AGCCACAGAG
    GAGGCCCAGG CCCTGGCCAT ACAGGCCGTG CTCCAGGCCG CACAGCAGGC TGTCATGGCA
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCAG CGGTGACACA GGCAGAGTTG
    AGCCACCTGT CGGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACA
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCACTG GCCCCGGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GAGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC CCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCAGCCACCC TGACGGAAGT GGCCAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA TGCTGTCCCG ACTGATGTAT GTATCCCTGG GAGCCAACAC
    CCTCAGGATG ACTCGGGCAC CGTGCCCGAC TACAACCAGC TAAAGAAGCA GGAGCTGCAG
    CCAGGCACTG CCTATAAGTT CCGCGTCGCC GGGATCAATG CCTGCGGCCG GGGGCCCTTC
    AGTGAGATCT CAGCCTTTAA AACGTGTCTG CCTGGCTTCC CAGGGGCCCC GTGTGCCATT
    AAAATCAGCA AAAGTCCAGA CGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC
    GGCAAGATCA TCGAGTACTC AGTGTACCTG GCCATCCAGA GCGCGCAGGC CGGTGGTGAG
    ACCAAGAGCT CGACCCCGGC GCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC
    TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC AACGCCCACA TCGACTACAC CACCAAGCCC
    GCCATCATCT TCCGCATTGC TGCCCGCAAT GAGAAGGGCT ACGGCCCAGC CACCCAAGTC
    AGGTGGTTGC AAGAAACCAG TAAAGACAGC TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC
    ATGTCGTCTC CAGAAATGAA ATCCGCGCCA AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48472
    Clone ID Related Accession (Same CDS sequence) XM_024988690.1
    Accession Version XM_024988690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CAGCGGTGAC ACAGGCAGAG
    TTGAGCCACC TGTCGGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACACAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCA CTGGCCCCGG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGAGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCAGCCA CCCTGACGGA AGTGGCCAAT
    GGCATTGAGT CCCTGGGTGT GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA TGCTGTCCCG ACTGATGTAT GTATCCCTGG GAGCCAACAC
    CCTCAGGATG ACTCGGGCAC CGTGCCCGAC TACAACCAGC TAAAGAAGCA GGAGCTGCAG
    CCAGGCACTG CCTATAAGTT CCGCGTCGCC GGGATCAATG CCTGCGGCCG GGGGCCCTTC
    AGTGAGATCT CAGCCTTTAA AACGTGTCTG CCTGGCTTCC CAGGGGCCCC GTGTGCCATT
    AAAATCAGCA AAAGTCCAGA CGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC
    GGCAAGATCA TCGAGTACTC AGTGTACCTG GCCATCCAGA GCGCGCAGGC CGGTGGTGAG
    ACCAAGAGCT CGACCCCGGC GCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC
    TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC AACGCCCACA TCGACTACAC CACCAAGCCC
    GCCATCATCT TCCGCATTGC TGCCCGCAAT GAGAAGGGCT ACGGCCCAGC CACCCAAGTC
    AGGTGGTTGC AAGAAACCAG TAAAGACAGC TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC
    ATGTCGTCTC CAGAAATGAA ATCCGCGCCA AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844458.1
    CDS1055..7231
    Translation

    Target ORF information:

    RefSeq Version XM_024988690.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CAGCGGTGAC ACAGGCAGAG
    TTGAGCCACC TGTCGGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACACAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCA CTGGCCCCGG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGAGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCAGCCA CCCTGACGGA AGTGGCCAAT
    GGCATTGAGT CCCTGGGTGT GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA TGCTGTCCCG ACTGATGTAT GTATCCCTGG GAGCCAACAC
    CCTCAGGATG ACTCGGGCAC CGTGCCCGAC TACAACCAGC TAAAGAAGCA GGAGCTGCAG
    CCAGGCACTG CCTATAAGTT CCGCGTCGCC GGGATCAATG CCTGCGGCCG GGGGCCCTTC
    AGTGAGATCT CAGCCTTTAA AACGTGTCTG CCTGGCTTCC CAGGGGCCCC GTGTGCCATT
    AAAATCAGCA AAAGTCCAGA CGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC
    GGCAAGATCA TCGAGTACTC AGTGTACCTG GCCATCCAGA GCGCGCAGGC CGGTGGTGAG
    ACCAAGAGCT CGACCCCGGC GCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC
    TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC AACGCCCACA TCGACTACAC CACCAAGCCC
    GCCATCATCT TCCGCATTGC TGCCCGCAAT GAGAAGGGCT ACGGCCCAGC CACCCAAGTC
    AGGTGGTTGC AAGAAACCAG TAAAGACAGC TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC
    ATGTCGTCTC CAGAAATGAA ATCCGCGCCA AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48473
    Clone ID Related Accession (Same CDS sequence) XM_015461524.2
    Accession Version XM_015461524.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CAGCGGTGAC ACAGGCAGAG
    TTGAGCCACC TGTCGGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACACAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCA CTGGCCCCGG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGAGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCAGCCA CCCTGACGGA AGTGGCCAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGATGCTGTC CCGACTGATG ATGACTCGGG CACCGTGCCC
    GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC
    GCCGGGATCA ATGCCTGCGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACGTGT
    CTGCCTGGCT TCCCAGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT
    CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCAGTGTAC
    CTGGCCATCC AGAGCGCGCA GGCCGGTGGT GAGACCAAGA GCTCGACCCC GGCGCAGCTG
    GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC
    TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT TGCTGCCCGC
    AATGAGAAGG GCTACGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC
    AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG
    CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317010.2
    CDS1053..7202
    Translation

    Target ORF information:

    RefSeq Version XM_015461524.2
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461524.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCA GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCC ACCCAGTCCG AAGTAGACCA GTTGTCCCTC
    CCCCAAGAGC TGATGGCCGA AGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCGCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATACAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CAGCGGTGAC ACAGGCAGAG
    TTGAGCCACC TGTCGGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACACAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCA CTGGCCCCGG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGAGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCCCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCAGCCA CCCTGACGGA AGTGGCCAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGATGCTGTC CCGACTGATG ATGACTCGGG CACCGTGCCC
    GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC
    GCCGGGATCA ATGCCTGCGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACGTGT
    CTGCCTGGCT TCCCAGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT
    CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCAGTGTAC
    CTGGCCATCC AGAGCGCGCA GGCCGGTGGT GAGACCAAGA GCTCGACCCC GGCGCAGCTG
    GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC
    TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT TGCTGCCCGC
    AATGAGAAGG GCTACGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC
    AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG
    CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48474
    Clone ID Related Accession (Same CDS sequence) XM_024988691.1
    Accession Version XM_024988691.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCCACCCAG
    TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAAGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATACA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCAGCGG TGACACAGGC AGAGTTGAGC CACCTGTCGG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACACAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCACTGGCC
    CCGGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGAG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCCCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCA
    GCCACCCTGA CGGAAGTGGC CAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGATGC TGTCCCGACT
    GATGTATGTA TCCCTGGGAG CCAACACCCT CAGGATGACT CGGGCACCGT GCCCGACTAC
    AACCAGCTAA AGAAGCAGGA GCTGCAGCCA GGCACTGCCT ATAAGTTCCG CGTCGCCGGG
    ATCAATGCCT GCGGCCGGGG GCCCTTCAGT GAGATCTCAG CCTTTAAAAC GTGTCTGCCT
    GGCTTCCCAG GGGCCCCGTG TGCCATTAAA ATCAGCAAAA GTCCAGACGG TGCTCACCTC
    ACCTGGGAGC CACCCTCTGT GACCTCCGGC AAGATCATCG AGTACTCAGT GTACCTGGCC
    ATCCAGAGCG CGCAGGCCGG TGGTGAGACC AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC
    ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC
    GCCCACATCG ACTACACCAC CAAGCCCGCC ATCATCTTCC GCATTGCTGC CCGCAATGAG
    AAGGGCTACG GCCCAGCCAC CCAAGTCAGG TGGTTGCAAG AAACCAGTAA AGACAGCTCT
    GGCGCCAAGC CGGCCAGCAA GCGGCCCATG TCGTCTCCAG AAATGAAATC CGCGCCAAAG
    AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844459.1
    CDS1053..7196
    Translation

    Target ORF information:

    RefSeq Version XM_024988691.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCCGG AACTGGAGCC CCCGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACCGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACACCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TTACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACGG TCACCATCGC TGATTCGGGC
    CAGGGTGATG TGCAGCCCGG CACCGTGACC CTGGTGTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTAGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCTACCACCG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCATGCCAA
    ACCCGTCAGA CCAGTGCAAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TCGGCTGGCC CACTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCCACT
    CTCTTGCAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AAGTGCATCA CACGCACACG
    ACCAACACGC CCACTGTGGC CCGCTCCGCC ATGGGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCAGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCGCCGTG ACCCAGGTCT GCTCCAACCC CCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCCGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC ACCCTGTGAG
    ACGCATGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACCCCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CCTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCAGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCCACCCAG
    TCCGAAGTAG ACCAGTTGTC CCTCCCCCAA GAGCTGATGG CCGAAGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCGC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATACA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCAGCGG TGACACAGGC AGAGTTGAGC CACCTGTCGG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACACAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCACTGGCC
    CCGGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGAG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCCCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCA
    GCCACCCTGA CGGAAGTGGC CAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGATGC TGTCCCGACT
    GATGTATGTA TCCCTGGGAG CCAACACCCT CAGGATGACT CGGGCACCGT GCCCGACTAC
    AACCAGCTAA AGAAGCAGGA GCTGCAGCCA GGCACTGCCT ATAAGTTCCG CGTCGCCGGG
    ATCAATGCCT GCGGCCGGGG GCCCTTCAGT GAGATCTCAG CCTTTAAAAC GTGTCTGCCT
    GGCTTCCCAG GGGCCCCGTG TGCCATTAAA ATCAGCAAAA GTCCAGACGG TGCTCACCTC
    ACCTGGGAGC CACCCTCTGT GACCTCCGGC AAGATCATCG AGTACTCAGT GTACCTGGCC
    ATCCAGAGCG CGCAGGCCGG TGGTGAGACC AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC
    ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC
    GCCCACATCG ACTACACCAC CAAGCCCGCC ATCATCTTCC GCATTGCTGC CCGCAATGAG
    AAGGGCTACG GCCCAGCCAC CCAAGTCAGG TGGTTGCAAG AAACCAGTAA AGACAGCTCT
    GGCGCCAAGC CGGCCAGCAA GCGGCCCATG TCGTCTCCAG AAATGAAATC CGCGCCAAAG
    AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48475
    Clone ID Related Accession (Same CDS sequence) XM_024988694.1
    Accession Version XM_024988694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG
    ATGGCCGAAG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TACAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCA GCGGTGACAC AGGCAGAGTT GAGCCACCTG
    TCGGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC ACAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCACT GGCCCCGGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGAGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CCCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCAGCCACC CTGACGGAAG TGGCCAATGG CATTGAGTCC
    CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC
    CAGTGGTTTG ACGTGGGGGT CATTAAGGGT ACCAATGTGA TGGTGACACA CTATTTCCTG
    CCACCCGAAG ATGCTGTCCC GACTGATGTA TGTATCCCTG GGAGCCAACA CCCTCAGGAT
    GACTCGGGCA CCGTGCCCGA CTACAACCAG CTAAAGAAGC AGGAGCTGCA GCCAGGCACT
    GCCTATAAGT TCCGCGTCGC CGGGATCAAT GCCTGCGGCC GGGGGCCCTT CAGTGAGATC
    TCAGCCTTTA AAACGTGTCT GCCTGGCTTC CCAGGGGCCC CGTGTGCCAT TAAAATCAGC
    AAAAGTCCAG ACGGTGCTCA CCTCACCTGG GAGCCACCCT CTGTGACCTC CGGCAAGATC
    ATCGAGTACT CAGTGTACCT GGCCATCCAG AGCGCGCAGG CCGGTGGTGA GACCAAGAGC
    TCGACCCCGG CGCAGCTGGC CTTCATGCGG GTGTACTGCG GGCCCAGCCC CTCCTGCCTC
    GTGCAGTCCT CCAGCCTCTC CAACGCCCAC ATCGACTACA CCACCAAGCC CGCCATCATC
    TTCCGCATTG CTGCCCGCAA TGAGAAGGGC TACGGCCCAG CCACCCAAGT CAGGTGGTTG
    CAAGAAACCA GTAAAGACAG CTCTGGCGCC AAGCCGGCCA GCAAGCGGCC CATGTCGTCT
    CCAGAAATGA AATCCGCGCC AAAGAAATCG AAGGCAGACG GTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844462.1
    CDS1054..7101
    Translation

    Target ORF information:

    RefSeq Version XM_024988694.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG
    ATGGCCGAAG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TACAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCA GCGGTGACAC AGGCAGAGTT GAGCCACCTG
    TCGGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC ACAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCACT GGCCCCGGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGAGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CCCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCAGCCACC CTGACGGAAG TGGCCAATGG CATTGAGTCC
    CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC
    CAGTGGTTTG ACGTGGGGGT CATTAAGGGT ACCAATGTGA TGGTGACACA CTATTTCCTG
    CCACCCGAAG ATGCTGTCCC GACTGATGTA TGTATCCCTG GGAGCCAACA CCCTCAGGAT
    GACTCGGGCA CCGTGCCCGA CTACAACCAG CTAAAGAAGC AGGAGCTGCA GCCAGGCACT
    GCCTATAAGT TCCGCGTCGC CGGGATCAAT GCCTGCGGCC GGGGGCCCTT CAGTGAGATC
    TCAGCCTTTA AAACGTGTCT GCCTGGCTTC CCAGGGGCCC CGTGTGCCAT TAAAATCAGC
    AAAAGTCCAG ACGGTGCTCA CCTCACCTGG GAGCCACCCT CTGTGACCTC CGGCAAGATC
    ATCGAGTACT CAGTGTACCT GGCCATCCAG AGCGCGCAGG CCGGTGGTGA GACCAAGAGC
    TCGACCCCGG CGCAGCTGGC CTTCATGCGG GTGTACTGCG GGCCCAGCCC CTCCTGCCTC
    GTGCAGTCCT CCAGCCTCTC CAACGCCCAC ATCGACTACA CCACCAAGCC CGCCATCATC
    TTCCGCATTG CTGCCCGCAA TGAGAAGGGC TACGGCCCAG CCACCCAAGT CAGGTGGTTG
    CAAGAAACCA GTAAAGACAG CTCTGGCGCC AAGCCGGCCA GCAAGCGGCC CATGTCGTCT
    CCAGAAATGA AATCCGCGCC AAAGAAATCG AAGGCAGACG GTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48476
    Clone ID Related Accession (Same CDS sequence) XM_024988695.1
    Accession Version XM_024988695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG
    ATGGCCGAAG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TACAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGG CACCGGGGAG
    CCCATGGATA CTTCAGAGGC GGCCGCAGCG GTGACACAGG CAGAGTTGAG CCACCTGTCG
    GCCGAGGGCC AGGAAGGCCA GGCGACCACT ATCCCCATCG TGCTGACACA GCAGGAGCTG
    GCTGCCCTGG TCCAGCAGCA GCAGCTCCAG GAAGCACAGG CCCAGCAGCA GCAGCACCAC
    CTGCCCACGG AAGCACTGGC CCCGGCTGAC AGCCTCAATG ACCCGACCAT CGAGAGCAAC
    TGCCTCAATG AGCTGGCCGG GGCTGTGCCC AGCACCGTGA GCCTGCTGCC CCCCACGGCC
    ACTGAGAGCC TGGCCCCGTC CAACACATTT GTGGCCCCCC AGCCGGTCGT TGTGGCCAGC
    CCCGCGAAGC TGCAGGCCGC AGCCACCCTG ACGGAAGTGG CCAATGGCAT TGAGTCCCTG
    GGTGTGAAGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG
    TGGTTTGACG TGGGGGTCAT TAAGGGTACC AATGTGATGG TGACACACTA TTTCCTGCCA
    CCCGAAGATG CTGTCCCGAC TGATGATGAC TCGGGCACCG TGCCCGACTA CAACCAGCTA
    AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC
    TGCGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CGTGTCTGCC TGGCTTCCCA
    GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG
    CCACCCTCTG TGACCTCCGG CAAGATCATC GAGTACTCAG TGTACCTGGC CATCCAGAGC
    GCGCAGGCCG GTGGTGAGAC CAAGAGCTCG ACCCCGGCGC AGCTGGCCTT CATGCGGGTG
    TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATC
    GACTACACCA CCAAGCCCGC CATCATCTTC CGCATTGCTG CCCGCAATGA GAAGGGCTAC
    GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG
    CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG
    GCAGACGGTC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844463.1
    CDS1053..7067
    Translation

    Target ORF information:

    RefSeq Version XM_024988695.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT TGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGACTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GCGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCCTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCTG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCCCTG
    GAGGTGAGCT GGGGGGCCGT GGCAACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC CTCCCTTCCC
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACAGTCAT TGGCAGCAGC
    CCGCAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACGGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACCGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CCGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCTACAG GCCCGGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACACAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCGGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCCGGAACT
    GGAGCCCCCG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACCG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACA
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATCAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTTAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACGGTCAC CATCGCTGAT TCGGGCCAGG GTGATGTGCA GCCCGGCACC
    GTGACCCTGG TGTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTAG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCTA CCACCGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCA TGCCAAACCC GTCAGACCAG TGCAACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTCGG CTGGCCCACT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCCACTCTCT TGCAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAAGT GCATCACACG CACACGACCA ACACGCCCAC TGTGGCCCGC
    TCCGCCATGG GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    GCCGTGACCC AGGTCTGCTC CAACCCCCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC CGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCACCC TGTGAGACGC ATGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACCCCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCCTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCAGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCCAC CCAGTCCGAA GTAGACCAGT TGTCCCTCCC CCAAGAGCTG
    ATGGCCGAAG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCGCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TACAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGG CACCGGGGAG
    CCCATGGATA CTTCAGAGGC GGCCGCAGCG GTGACACAGG CAGAGTTGAG CCACCTGTCG
    GCCGAGGGCC AGGAAGGCCA GGCGACCACT ATCCCCATCG TGCTGACACA GCAGGAGCTG
    GCTGCCCTGG TCCAGCAGCA GCAGCTCCAG GAAGCACAGG CCCAGCAGCA GCAGCACCAC
    CTGCCCACGG AAGCACTGGC CCCGGCTGAC AGCCTCAATG ACCCGACCAT CGAGAGCAAC
    TGCCTCAATG AGCTGGCCGG GGCTGTGCCC AGCACCGTGA GCCTGCTGCC CCCCACGGCC
    ACTGAGAGCC TGGCCCCGTC CAACACATTT GTGGCCCCCC AGCCGGTCGT TGTGGCCAGC
    CCCGCGAAGC TGCAGGCCGC AGCCACCCTG ACGGAAGTGG CCAATGGCAT TGAGTCCCTG
    GGTGTGAAGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG
    TGGTTTGACG TGGGGGTCAT TAAGGGTACC AATGTGATGG TGACACACTA TTTCCTGCCA
    CCCGAAGATG CTGTCCCGAC TGATGATGAC TCGGGCACCG TGCCCGACTA CAACCAGCTA
    AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC
    TGCGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CGTGTCTGCC TGGCTTCCCA
    GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG
    CCACCCTCTG TGACCTCCGG CAAGATCATC GAGTACTCAG TGTACCTGGC CATCCAGAGC
    GCGCAGGCCG GTGGTGAGAC CAAGAGCTCG ACCCCGGCGC AGCTGGCCTT CATGCGGGTG
    TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATC
    GACTACACCA CCAAGCCCGC CATCATCTTC CGCATTGCTG CCCGCAATGA GAAGGGCTAC
    GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG
    CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG
    GCAGACGGTC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89280
    Clone ID Related Accession (Same CDS sequence) XM_010800388.2 , XM_015461524.1
    Accession Version XM_010800388.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647006.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGGAAT ACGGGAAGTA CAGCAACGAC CTCTACGAGC TCCAGGCAAG CCGGTGGGAA 
    TGGAAGAGAC TGAAAGCAAA GACGCCCAAA AATGGGCCAC CTCCGTGTCC TCGGCTTGGG
    CACAGCTTCT CCCTTGTGGG CAACAAGTGT TACCTGTTTG GGGGTCTGGC CAATGATAGC
    GAGGACCCCA AGAATAACAT TCCGAGGTAC CTGAATGACT TATACATCCT GGAACTGCGG
    CCGGGCTCTG GAGTGGTAGC CTGGGACATT CCCATCACTT ACGGCGTCCT GCCCCCACCC
    CGAGAGTCTC ACACTGCAGT GGTCTACACT GAGAAAGACA ACAAGAAGTC CAAACTGGTG
    ATCTATGGAG GGATGAGTGG CTGCAGGCTG GGGGACCTCT GGACCTTGGA TATCGAGACT
    TTGACATGGA ATAAGCCCAG CCTCAGCGGG GTGGCACCTC TTCCTCGAAG TCTCCACTCG
    GCCACGACCA TAGGAAACAA AATGTACGTG TTTGGTGGCT GGGTGCCTCT CGTCATGGAT
    GACGTCAAAG TGGCCACACA CGAGAAGGAG TGGAAGTGTA CCAACACTCT GGCTTGTCTC
    AACCTGGATA CCATGGCTTG GGAGACCATC CTGATGGATA CGCTGGAAGA CAATATTCCC
    CGGGCCCGTG CTGGCCACTG TGCGGTAGCC ATCAATACCC GCCTTTACAT TTGGAGTGGG
    CGTGACGGCT ACCGAAAGGC CTGGAATAAC CAGGTCTGCT GCAAGGACCT CTGGTACCTG
    GAAACGGAGA AGCCGCCACC TCCAGCCCGG GTACAGCTGG TGCGAGCCAA CACTAACTCC
    CTGGAGGTGA GCTGGGGGGC CGTGGCAACA GCCGACAGTT ACCTTCTGCA GCTCCAGAAA
    TATGACATTC CTGCCACGGC TGCCACTGCC ACCTCCCCCA CACCCAATCC AGTCCCGTCT
    GTGCCTGCCA ATCCTCCCAA GAGCCCTGCC CCAGCAGCAG CCGCACCTGC CGTGCAGCCG
    CTGACCCAAG TAGGCATCAC GCTCCTGCCC CAGGCTGCTG CCGCGCCCCC GACCACCACC
    ACCATCCAGG TCTTGCCGAC GGTGCCTGGC AGCTCAATCT CCGTGCCCGC CGCAGCCAGG
    ACTCAAGGTG TCCCGGCTGT GCTGAAAGTG ACTGGTCCTC AGGCTACCAC GGGAACCCCG
    TTGGTCACCA TGCGACCTGC CAGCCAGGCT GGGAAAGCCC CCGTGACTGT GACCTCCCTT
    CCCGCAGGCG TGCGGATGGT TGTGCCTACA CAGAGCGCCC AGGGGACAGT CATTGGCAGC
    AGCCCGCAGA TGAGTGGCAT GGCTGCATTG GCAGCCGCGG CTGCTGCCAC CCAGAAGATC
    CCTCCTTCCT CGGCACCCAC GGTCCTGAGT GTCCCGGCTG GCACCACTAT CGTCAAAACC
    GTGGCCGTGA CGCCGGGCAC CACCACGCTC CCAGCCACTG TGAAGGTGGC CTCCTCGCCA
    GTCATGGTGA GCAACCCAGC CACTCGCATG CTGAAGACTG CAGCTGCCCA GGTGGGGACT
    TCCGTCTCTT CTGCCGCCAA CACATCTACC CGTCCCATCA TCACCGTACA CAAGTCCGGA
    ACCGTGACAG TGGCCCAGCA AGCCCAGGTG GTGACCACAG TGGTGGGTGG GGTTACCAAG
    ACCATCACCC TGGTGAAGAG CCCCATCTCG GTCCCAGGAG GCAGTGCTCT GATTTCCAAT
    CTGGGCAAAG TAATGTCAGT GGTCCAGACC AAACCGGTTC AGACTTCGGC AGTCACAGGC
    CAGGCATCTA CAGGCCCGGT GACTCAGATC ATCCAGACCA AAGGACCCTT GCCAGCCGGG
    ACCATCCTGA AGCTGGTGAC GTCTGCAGAT GGCAAGCCCA CTACCATCAT CACAACCACA
    CAGGCCAGCG GGGCAGGGAC TAAGCCTACC ATCCTGGGCA TCAGCAGTGT GTCCCCCAGC
    ACCACCAAGC CCGGCACCAC CACTATCATT AAGACCATCC CCATGTCGGC CATCATCACA
    CAGGCGGGCG CCACGGGTAG GGGCCTTCCC CGGTGTGCTG GGGAGAAAGC AGGCGTGACT
    AGCAGTGCCG GCATCAAGTC ACCCATCACC ATTATCACCA CCAAGGTGAT GACTTCCGGA
    ACTGGAGCCC CCGCCAAAAT CATCACGGCT GTTCCCAAAA TCGCTACGGG TCACGGGCAG
    CAAGGAGTGA CCCAGGTGGT GCTGAAGGGC GCACCTGGCC AGCCAGGCAC CATCCTCCGC
    ACCGTGCCTA TGGGGGGCGT CCGCCTGGTC ACCCCTGTTA CCGTCTCTGC TGTCAAGCCT
    GCTGTCACCA CACTGGTTGT CAAGGGCACC ACAGGCGTCA CGACCCTGGG CACGGTGACA
    GGCACCGTCT CCACCAGCCT GGCCGGAGCC GGGGGCCATA GCACCAGCGC CTCACTGGCC
    ACACCCATCA CCACGTTGGG CACCATCGCC ACCCTCTCAA GCCAGGTGAT CAACCCCACT
    GCCATCACTG TGTCTGCGGC GCAGACGACG CTGACAGCGG CCGCTGGGCT TACCACACCC
    ACCATAACCA TGCAGCCTGT CTCCCAGCCT ACCCAGGTGA CTCTGATAAC GGCCCCCAGC
    GGGGTGGAGG CCCAGCCTGT GCACGACCTC CCAGTGTCCA TTCTGGCCTC CCCTACTACA
    GAACAGCCCA CGGCCACGGT CACCATCGCT GATTCGGGCC AGGGTGATGT GCAGCCCGGC
    ACCGTGACCC TGGTGTGCTC CAACCCGCCG TGCGAAACCC ACGAGACGGG CACCACCAAT
    ACAGCCACCA CCACCGTCGT CGCTAATCTG GGGGGGCACC CCCAGCCCAC CCAAGTGCAG
    TTTGTCTGCG ACAGACAGGA GGCCACTGCT GCTCTCGTGA CCTCCACGGT GGGCCAGCCG
    AATGGCAGTG TAGTTCGTGT CTGCTCCAAC CCGCCGTGTG AAACCCACGA GACCGGCACC
    ACCAGTACAG CTACCACCGC CATGTCTGGC ATCGGAGGCG GGCCGCGGCG AGACATCCGG
    CTCGCCTGCG CGGCCACCAC CATTCCCACC GTGGTCCGGG TCAGCGTGAC TGCCGGGGCG
    TCAGAGGGAG CTCAGGTTTC CATCAAGCCC GCATGCCAAA CCCGTCAGAC CAGTGCAACC
    AGCACCACCA TGACTGTGAT GGCCACCGGG GCCCCGTGCT CGGCTGGCCC ACTCCTCAGA
    CCAAGCGTGG CCCTCGAGGC CGCTGGCCGC GGTGCCACTC TCTTGCAGCT GGGGCCACTG
    AGTGCCCAAG TCAGGCCCGG TGGCGAGGAA AGGTCCCTTG CCGGCCTGGG CCCGCTGGTG
    TCTGTGGGGC GCCAGCTGGA AGTGCATCAC ACGCACACGA CCAACACGCC CACTGTGGCC
    CGCTCCGCCA TGGGTGCCGG GGAGCCCCAC GAGCTGCTGG GGGCCCCCAC ACTTGTGTAC
    GAGAGCTCAG CCAGCGCCTC TGTGACTGCC GCAGCCCTGG AGGCACTGCT GTGCCCCTCG
    GCCGCCGTGA CCCAGGTCTG CTCCAACCCC CCGTGTGAGA CCCACGAGAC GGGCACCACC
    CACACACCCA CCACGGCCAC ATCCGGAGGG GCTGCAGGCC AGCCAGAGGG CGGGCAGCAG
    CCTCCTGCCA GCCGGCCCTG TGAGACGCAC CAGACCGCTT CCACTGGCAC GACCATGTCC
    GTCAGCTTGG GCGCCCTGCT CCCGGATGCC GCCCCCTCCC ACAGGACCCT GGAGTCCAGC
    CTGGAGGTGG CAGTGCCACC CGCAGTCGCC CCTCAGGCCG GTGCTTCGTT GCTCACTCCT
    TTCCCGACGC AAAGGGTGTG CTCCAACCCA CCCTGTGAGA CGCATGAGAC AGGCACTACG
    CACACGGCCA CCACTGTCAC CTCCAACATG AGTTCCAACC AAGACCCCCC ACCGCCTGCC
    GGCGACCAGG GAGAGGTGGA GAGCACCCAG GGCGACAGTG TGAATATCGC CAGTTCCAGT
    CCCATCACGA CAACAGTGTC CTCCACGCTG ACGCGGGCCG TGACCACCGT GACACAGTCC
    ACGCCAGTCC CAGGCCCTTC GGTGCCGAAG ATCTCATCTG TGACTGAGAC TGCCCCAGGG
    GCTCTGACCA CCGAAGTCCC CATCCCGGCC ACGATTACAG TGACCATAGC CAACACAGAA
    ACTTCTGACA TGCCCTTCTC TGCTGTTGAC ATCCTGCAGC CCCCAGAGGA GCTCCAGGCC
    TCTCCAGGGC CACGCCAGCA GCTTCCACCA CGGCAGCTCC TGCAGCCTGC CTCCGCGCCC
    TTGGTGGGGG ACTCCGCCGA GGTCCTGTCA GCCTCCCAGA GCCCTGAGCT CCAGGCCGCC
    GTGGATCTGA GCAGTACAGG GGACCCGTCT TCAGGCCAGG AGCCTGCCAG CTCGGCTGTG
    GTGGCCACCG TGGTGGTCCA GCCACCGCCG CCCACCCAGT CCGAAGTAGA CCAGTTGTCC
    CTCCCCCAAG AGCTGATGGC CGAAGCCCAG GCGGGCACCA CCACCCTCAT GGTGACAGGG
    CTCACCCCAG AGGAGCTGGC AGTGACTGCT GCTGCTGAAG CGGCCGCGCA GGCTGCAGCC
    ACAGAGGAGG CCCAGGCCCT GGCCATACAG GCCGTGCTCC AGGCCGCACA GCAGGCTGTC
    ATGGCAGGCA CCGGGGAGCC CATGGATACT TCAGAGGCGG CCGCAGCGGT GACACAGGCA
    GAGTTGAGCC ACCTGTCGGC CGAGGGCCAG GAAGGCCAGG CGACCACTAT CCCCATCGTG
    CTGACACAGC AGGAGCTGGC TGCCCTGGTC CAGCAGCAGC AGCTCCAGGA AGCACAGGCC
    CAGCAGCAGC AGCACCACCT GCCCACGGAA GCACTGGCCC CGGCTGACAG CCTCAATGAC
    CCGACCATCG AGAGCAACTG CCTCAATGAG CTGGCCGGGG CTGTGCCCAG CACCGTGAGC
    CTGCTGCCCC CCACGGCCAC TGAGAGCCTG GCCCCGTCCA ACACATTTGT GGCCCCCCAG
    CCGGTCGTTG TGGCCAGCCC CGCGAAGCTG CAGGCCGCAG CCACCCTGAC GGAAGTGGCC
    AATGGCATTG AGTCCCTGGG TGTGAAGCCA GACCTGCCAC CGCCACCTAG CAAAGCCCCT
    GTAAAGAAGG AGAACCAGTG GTTTGACGTG GGGGTCATTA AGGGTACCAA TGTGATGGTG
    ACACACTATT TCCTGCCACC CGAAGATGCT GTCCCGACTG ATGATGACTC GGGCACCGTG
    CCCGACTACA ACCAGCTAAA GAAGCAGGAG CTGCAGCCAG GCACTGCCTA TAAGTTCCGC
    GTCGCCGGGA TCAATGCCTG CGGCCGGGGG CCCTTCAGTG AGATCTCAGC CTTTAAAACG
    TGTCTGCCTG GCTTCCCAGG GGCCCCGTGT GCCATTAAAA TCAGCAAAAG TCCAGACGGT
    GCTCACCTCA CCTGGGAGCC ACCCTCTGTG ACCTCCGGCA AGATCATCGA GTACTCAGTG
    TACCTGGCCA TCCAGAGCGC GCAGGCCGGT GGTGAGACCA AGAGCTCGAC CCCGGCGCAG
    CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC AGCCCCTCCT GCCTCGTGCA GTCCTCCAGC
    CTCTCCAACG CCCACATCGA CTACACCACC AAGCCCGCCA TCATCTTCCG CATTGCTGCC
    CGCAATGAGA AGGGCTACGG CCCAGCCACC CAAGTCAGGT GGTTGCAAGA AACCAGTAAA
    GACAGCTCTG GCGCCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCC
    GCGCCAAAGA AATCGAAGGC AGACGGTCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010798690.2
    CDS1371..7223
    Translation

    Target ORF information:

    RefSeq Version XM_010800388.2
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800388.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGGAAT ACGGGAAGTA CAGCAACGAC CTCTACGAGC TCCAGGCAAG CCGGTGGGAA 
    TGGAAGAGAC TGAAAGCAAA GACGCCCAAA AATGGGCCAC CTCCGTGTCC TCGGCTTGGG
    CACAGCTTCT CCCTTGTGGG CAACAAGTGT TACCTGTTTG GGGGTCTGGC CAATGATAGC
    GAGGACCCCA AGAATAACAT TCCGAGGTAC CTGAATGACT TATACATCCT GGAACTGCGG
    CCGGGCTCTG GAGTGGTAGC CTGGGACATT CCCATCACTT ACGGCGTCCT GCCCCCACCC
    CGAGAGTCTC ACACTGCAGT GGTCTACACT GAGAAAGACA ACAAGAAGTC CAAACTGGTG
    ATCTATGGAG GGATGAGTGG CTGCAGGCTG GGGGACCTCT GGACCTTGGA TATCGAGACT
    TTGACATGGA ATAAGCCCAG CCTCAGCGGG GTGGCACCTC TTCCTCGAAG TCTCCACTCG
    GCCACGACCA TAGGAAACAA AATGTACGTG TTTGGTGGCT GGGTGCCTCT CGTCATGGAT
    GACGTCAAAG TGGCCACACA CGAGAAGGAG TGGAAGTGTA CCAACACTCT GGCTTGTCTC
    AACCTGGATA CCATGGCTTG GGAGACCATC CTGATGGATA CGCTGGAAGA CAATATTCCC
    CGGGCCCGTG CTGGCCACTG TGCGGTAGCC ATCAATACCC GCCTTTACAT TTGGAGTGGG
    CGTGACGGCT ACCGAAAGGC CTGGAATAAC CAGGTCTGCT GCAAGGACCT CTGGTACCTG
    GAAACGGAGA AGCCGCCACC TCCAGCCCGG GTACAGCTGG TGCGAGCCAA CACTAACTCC
    CTGGAGGTGA GCTGGGGGGC CGTGGCAACA GCCGACAGTT ACCTTCTGCA GCTCCAGAAA
    TATGACATTC CTGCCACGGC TGCCACTGCC ACCTCCCCCA CACCCAATCC AGTCCCGTCT
    GTGCCTGCCA ATCCTCCCAA GAGCCCTGCC CCAGCAGCAG CCGCACCTGC CGTGCAGCCG
    CTGACCCAAG TAGGCATCAC GCTCCTGCCC CAGGCTGCTG CCGCGCCCCC GACCACCACC
    ACCATCCAGG TCTTGCCGAC GGTGCCTGGC AGCTCAATCT CCGTGCCCGC CGCAGCCAGG
    ACTCAAGGTG TCCCGGCTGT GCTGAAAGTG ACTGGTCCTC AGGCTACCAC GGGAACCCCG
    TTGGTCACCA TGCGACCTGC CAGCCAGGCT GGGAAAGCCC CCGTGACTGT GACCTCCCTT
    CCCGCAGGCG TGCGGATGGT TGTGCCTACA CAGAGCGCCC AGGGGACAGT CATTGGCAGC
    AGCCCGCAGA TGAGTGGCAT GGCTGCATTG GCAGCCGCGG CTGCTGCCAC CCAGAAGATC
    CCTCCTTCCT CGGCACCCAC GGTCCTGAGT GTCCCGGCTG GCACCACTAT CGTCAAAACC
    GTGGCCGTGA CGCCGGGCAC CACCACGCTC CCAGCCACTG TGAAGGTGGC CTCCTCGCCA
    GTCATGGTGA GCAACCCAGC CACTCGCATG CTGAAGACTG CAGCTGCCCA GGTGGGGACT
    TCCGTCTCTT CTGCCGCCAA CACATCTACC CGTCCCATCA TCACCGTACA CAAGTCCGGA
    ACCGTGACAG TGGCCCAGCA AGCCCAGGTG GTGACCACAG TGGTGGGTGG GGTTACCAAG
    ACCATCACCC TGGTGAAGAG CCCCATCTCG GTCCCAGGAG GCAGTGCTCT GATTTCCAAT
    CTGGGCAAAG TAATGTCAGT GGTCCAGACC AAACCGGTTC AGACTTCGGC AGTCACAGGC
    CAGGCATCTA CAGGCCCGGT GACTCAGATC ATCCAGACCA AAGGACCCTT GCCAGCCGGG
    ACCATCCTGA AGCTGGTGAC GTCTGCAGAT GGCAAGCCCA CTACCATCAT CACAACCACA
    CAGGCCAGCG GGGCAGGGAC TAAGCCTACC ATCCTGGGCA TCAGCAGTGT GTCCCCCAGC
    ACCACCAAGC CCGGCACCAC CACTATCATT AAGACCATCC CCATGTCGGC CATCATCACA
    CAGGCGGGCG CCACGGGTAG GGGCCTTCCC CGGTGTGCTG GGGAGAAAGC AGGCGTGACT
    AGCAGTGCCG GCATCAAGTC ACCCATCACC ATTATCACCA CCAAGGTGAT GACTTCCGGA
    ACTGGAGCCC CCGCCAAAAT CATCACGGCT GTTCCCAAAA TCGCTACGGG TCACGGGCAG
    CAAGGAGTGA CCCAGGTGGT GCTGAAGGGC GCACCTGGCC AGCCAGGCAC CATCCTCCGC
    ACCGTGCCTA TGGGGGGCGT CCGCCTGGTC ACCCCTGTTA CCGTCTCTGC TGTCAAGCCT
    GCTGTCACCA CACTGGTTGT CAAGGGCACC ACAGGCGTCA CGACCCTGGG CACGGTGACA
    GGCACCGTCT CCACCAGCCT GGCCGGAGCC GGGGGCCATA GCACCAGCGC CTCACTGGCC
    ACACCCATCA CCACGTTGGG CACCATCGCC ACCCTCTCAA GCCAGGTGAT CAACCCCACT
    GCCATCACTG TGTCTGCGGC GCAGACGACG CTGACAGCGG CCGCTGGGCT TACCACACCC
    ACCATAACCA TGCAGCCTGT CTCCCAGCCT ACCCAGGTGA CTCTGATAAC GGCCCCCAGC
    GGGGTGGAGG CCCAGCCTGT GCACGACCTC CCAGTGTCCA TTCTGGCCTC CCCTACTACA
    GAACAGCCCA CGGCCACGGT CACCATCGCT GATTCGGGCC AGGGTGATGT GCAGCCCGGC
    ACCGTGACCC TGGTGTGCTC CAACCCGCCG TGCGAAACCC ACGAGACGGG CACCACCAAT
    ACAGCCACCA CCACCGTCGT CGCTAATCTG GGGGGGCACC CCCAGCCCAC CCAAGTGCAG
    TTTGTCTGCG ACAGACAGGA GGCCACTGCT GCTCTCGTGA CCTCCACGGT GGGCCAGCCG
    AATGGCAGTG TAGTTCGTGT CTGCTCCAAC CCGCCGTGTG AAACCCACGA GACCGGCACC
    ACCAGTACAG CTACCACCGC CATGTCTGGC ATCGGAGGCG GGCCGCGGCG AGACATCCGG
    CTCGCCTGCG CGGCCACCAC CATTCCCACC GTGGTCCGGG TCAGCGTGAC TGCCGGGGCG
    TCAGAGGGAG CTCAGGTTTC CATCAAGCCC GCATGCCAAA CCCGTCAGAC CAGTGCAACC
    AGCACCACCA TGACTGTGAT GGCCACCGGG GCCCCGTGCT CGGCTGGCCC ACTCCTCAGA
    CCAAGCGTGG CCCTCGAGGC CGCTGGCCGC GGTGCCACTC TCTTGCAGCT GGGGCCACTG
    AGTGCCCAAG TCAGGCCCGG TGGCGAGGAA AGGTCCCTTG CCGGCCTGGG CCCGCTGGTG
    TCTGTGGGGC GCCAGCTGGA AGTGCATCAC ACGCACACGA CCAACACGCC CACTGTGGCC
    CGCTCCGCCA TGGGTGCCGG GGAGCCCCAC GAGCTGCTGG GGGCCCCCAC ACTTGTGTAC
    GAGAGCTCAG CCAGCGCCTC TGTGACTGCC GCAGCCCTGG AGGCACTGCT GTGCCCCTCG
    GCCGCCGTGA CCCAGGTCTG CTCCAACCCC CCGTGTGAGA CCCACGAGAC GGGCACCACC
    CACACACCCA CCACGGCCAC ATCCGGAGGG GCTGCAGGCC AGCCAGAGGG CGGGCAGCAG
    CCTCCTGCCA GCCGGCCCTG TGAGACGCAC CAGACCGCTT CCACTGGCAC GACCATGTCC
    GTCAGCTTGG GCGCCCTGCT CCCGGATGCC GCCCCCTCCC ACAGGACCCT GGAGTCCAGC
    CTGGAGGTGG CAGTGCCACC CGCAGTCGCC CCTCAGGCCG GTGCTTCGTT GCTCACTCCT
    TTCCCGACGC AAAGGGTGTG CTCCAACCCA CCCTGTGAGA CGCATGAGAC AGGCACTACG
    CACACGGCCA CCACTGTCAC CTCCAACATG AGTTCCAACC AAGACCCCCC ACCGCCTGCC
    GGCGACCAGG GAGAGGTGGA GAGCACCCAG GGCGACAGTG TGAATATCGC CAGTTCCAGT
    CCCATCACGA CAACAGTGTC CTCCACGCTG ACGCGGGCCG TGACCACCGT GACACAGTCC
    ACGCCAGTCC CAGGCCCTTC GGTGCCGAAG ATCTCATCTG TGACTGAGAC TGCCCCAGGG
    GCTCTGACCA CCGAAGTCCC CATCCCGGCC ACGATTACAG TGACCATAGC CAACACAGAA
    ACTTCTGACA TGCCCTTCTC TGCTGTTGAC ATCCTGCAGC CCCCAGAGGA GCTCCAGGCC
    TCTCCAGGGC CACGCCAGCA GCTTCCACCA CGGCAGCTCC TGCAGCCTGC CTCCGCGCCC
    TTGGTGGGGG ACTCCGCCGA GGTCCTGTCA GCCTCCCAGA GCCCTGAGCT CCAGGCCGCC
    GTGGATCTGA GCAGTACAGG GGACCCGTCT TCAGGCCAGG AGCCTGCCAG CTCGGCTGTG
    GTGGCCACCG TGGTGGTCCA GCCACCGCCG CCCACCCAGT CCGAAGTAGA CCAGTTGTCC
    CTCCCCCAAG AGCTGATGGC CGAAGCCCAG GCGGGCACCA CCACCCTCAT GGTGACAGGG
    CTCACCCCAG AGGAGCTGGC AGTGACTGCT GCTGCTGAAG CGGCCGCGCA GGCTGCAGCC
    ACAGAGGAGG CCCAGGCCCT GGCCATACAG GCCGTGCTCC AGGCCGCACA GCAGGCTGTC
    ATGGCAGGCA CCGGGGAGCC CATGGATACT TCAGAGGCGG CCGCAGCGGT GACACAGGCA
    GAGTTGAGCC ACCTGTCGGC CGAGGGCCAG GAAGGCCAGG CGACCACTAT CCCCATCGTG
    CTGACACAGC AGGAGCTGGC TGCCCTGGTC CAGCAGCAGC AGCTCCAGGA AGCACAGGCC
    CAGCAGCAGC AGCACCACCT GCCCACGGAA GCACTGGCCC CGGCTGACAG CCTCAATGAC
    CCGACCATCG AGAGCAACTG CCTCAATGAG CTGGCCGGGG CTGTGCCCAG CACCGTGAGC
    CTGCTGCCCC CCACGGCCAC TGAGAGCCTG GCCCCGTCCA ACACATTTGT GGCCCCCCAG
    CCGGTCGTTG TGGCCAGCCC CGCGAAGCTG CAGGCCGCAG CCACCCTGAC GGAAGTGGCC
    AATGGCATTG AGTCCCTGGG TGTGAAGCCA GACCTGCCAC CGCCACCTAG CAAAGCCCCT
    GTAAAGAAGG AGAACCAGTG GTTTGACGTG GGGGTCATTA AGGGTACCAA TGTGATGGTG
    ACACACTATT TCCTGCCACC CGAAGATGCT GTCCCGACTG ATGATGACTC GGGCACCGTG
    CCCGACTACA ACCAGCTAAA GAAGCAGGAG CTGCAGCCAG GCACTGCCTA TAAGTTCCGC
    GTCGCCGGGA TCAATGCCTG CGGCCGGGGG CCCTTCAGTG AGATCTCAGC CTTTAAAACG
    TGTCTGCCTG GCTTCCCAGG GGCCCCGTGT GCCATTAAAA TCAGCAAAAG TCCAGACGGT
    GCTCACCTCA CCTGGGAGCC ACCCTCTGTG ACCTCCGGCA AGATCATCGA GTACTCAGTG
    TACCTGGCCA TCCAGAGCGC GCAGGCCGGT GGTGAGACCA AGAGCTCGAC CCCGGCGCAG
    CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC AGCCCCTCCT GCCTCGTGCA GTCCTCCAGC
    CTCTCCAACG CCCACATCGA CTACACCACC AAGCCCGCCA TCATCTTCCG CATTGCTGCC
    CGCAATGAGA AGGGCTACGG CCCAGCCACC CAAGTCAGGT GGTTGCAAGA AACCAGTAAA
    GACAGCTCTG GCGCCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCC
    GCGCCAAAGA AATCGAAGGC AGACGGTCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89280
    Clone ID Related Accession (Same CDS sequence) XM_010800388.2 , XM_015461524.1
    Accession Version XM_015461524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGGAAT ACGGGAAGTA CAGCAACGAC CTCTACGAGC TCCAGGCAAG CCGGTGGGAA 
    TGGAAGAGAC TGAAAGCAAA GACGCCCAAA AATGGGCCAC CTCCGTGTCC TCGGCTTGGG
    CACAGCTTCT CCCTTGTGGG CAACAAGTGT TACCTGTTTG GGGGTCTGGC CAATGATAGC
    GAGGACCCCA AGAATAACAT TCCGAGGTAC CTGAATGACT TATACATCCT GGAACTGCGG
    CCGGGCTCTG GAGTGGTAGC CTGGGACATT CCCATCACTT ACGGCGTCCT GCCCCCACCC
    CGAGAGTCTC ACACTGCAGT GGTCTACACT GAGAAAGACA ACAAGAAGTC CAAACTGGTG
    ATCTATGGAG GGATGAGTGG CTGCAGGCTG GGGGACCTCT GGACCTTGGA TATCGAGACT
    TTGACATGGA ATAAGCCCAG CCTCAGCGGG GTGGCACCTC TTCCTCGAAG TCTCCACTCG
    GCCACGACCA TAGGAAACAA AATGTACGTG TTTGGTGGCT GGGTGCCTCT CGTCATGGAT
    GACGTCAAAG TGGCCACACA CGAGAAGGAG TGGAAGTGTA CCAACACTCT GGCTTGTCTC
    AACCTGGATA CCATGGCTTG GGAGACCATC CTGATGGATA CGCTGGAAGA CAATATTCCC
    CGGGCCCGTG CTGGCCACTG TGCGGTAGCC ATCAATACCC GCCTTTACAT TTGGAGTGGG
    CGTGACGGCT ACCGAAAGGC CTGGAATAAC CAGGTCTGCT GCAAGGACCT CTGGTACCTG
    GAAACGGAGA AGCCGCCACC TCCAGCCCGG GTACAGCTGG TGCGAGCCAA CACTAACTCC
    CTGGAGGTGA GCTGGGGGGC CGTGGCAACA GCCGACAGTT ACCTTCTGCA GCTCCAGAAA
    TATGACATTC CTGCCACGGC TGCCACTGCC ACCTCCCCCA CACCCAATCC AGTCCCGTCT
    GTGCCTGCCA ATCCTCCCAA GAGCCCTGCC CCAGCAGCAG CCGCACCTGC CGTGCAGCCG
    CTGACCCAAG TAGGCATCAC GCTCCTGCCC CAGGCTGCTG CCGCGCCCCC GACCACCACC
    ACCATCCAGG TCTTGCCGAC GGTGCCTGGC AGCTCAATCT CCGTGCCCGC CGCAGCCAGG
    ACTCAAGGTG TCCCGGCTGT GCTGAAAGTG ACTGGTCCTC AGGCTACCAC GGGAACCCCG
    TTGGTCACCA TGCGACCTGC CAGCCAGGCT GGGAAAGCCC CCGTGACTGT GACCTCCCTT
    CCCGCAGGCG TGCGGATGGT TGTGCCTACA CAGAGCGCCC AGGGGACAGT CATTGGCAGC
    AGCCCGCAGA TGAGTGGCAT GGCTGCATTG GCAGCCGCGG CTGCTGCCAC CCAGAAGATC
    CCTCCTTCCT CGGCACCCAC GGTCCTGAGT GTCCCGGCTG GCACCACTAT CGTCAAAACC
    GTGGCCGTGA CGCCGGGCAC CACCACGCTC CCAGCCACTG TGAAGGTGGC CTCCTCGCCA
    GTCATGGTGA GCAACCCAGC CACTCGCATG CTGAAGACTG CAGCTGCCCA GGTGGGGACT
    TCCGTCTCTT CTGCCGCCAA CACATCTACC CGTCCCATCA TCACCGTACA CAAGTCCGGA
    ACCGTGACAG TGGCCCAGCA AGCCCAGGTG GTGACCACAG TGGTGGGTGG GGTTACCAAG
    ACCATCACCC TGGTGAAGAG CCCCATCTCG GTCCCAGGAG GCAGTGCTCT GATTTCCAAT
    CTGGGCAAAG TAATGTCAGT GGTCCAGACC AAACCGGTTC AGACTTCGGC AGTCACAGGC
    CAGGCATCTA CAGGCCCGGT GACTCAGATC ATCCAGACCA AAGGACCCTT GCCAGCCGGG
    ACCATCCTGA AGCTGGTGAC GTCTGCAGAT GGCAAGCCCA CTACCATCAT CACAACCACA
    CAGGCCAGCG GGGCAGGGAC TAAGCCTACC ATCCTGGGCA TCAGCAGTGT GTCCCCCAGC
    ACCACCAAGC CCGGCACCAC CACTATCATT AAGACCATCC CCATGTCGGC CATCATCACA
    CAGGCGGGCG CCACGGGTAG GGGCCTTCCC CGGTGTGCTG GGGAGAAAGC AGGCGTGACT
    AGCAGTGCCG GCATCAAGTC ACCCATCACC ATTATCACCA CCAAGGTGAT GACTTCCGGA
    ACTGGAGCCC CCGCCAAAAT CATCACGGCT GTTCCCAAAA TCGCTACGGG TCACGGGCAG
    CAAGGAGTGA CCCAGGTGGT GCTGAAGGGC GCACCTGGCC AGCCAGGCAC CATCCTCCGC
    ACCGTGCCTA TGGGGGGCGT CCGCCTGGTC ACCCCTGTTA CCGTCTCTGC TGTCAAGCCT
    GCTGTCACCA CACTGGTTGT CAAGGGCACC ACAGGCGTCA CGACCCTGGG CACGGTGACA
    GGCACCGTCT CCACCAGCCT GGCCGGAGCC GGGGGCCATA GCACCAGCGC CTCACTGGCC
    ACACCCATCA CCACGTTGGG CACCATCGCC ACCCTCTCAA GCCAGGTGAT CAACCCCACT
    GCCATCACTG TGTCTGCGGC GCAGACGACG CTGACAGCGG CCGCTGGGCT TACCACACCC
    ACCATAACCA TGCAGCCTGT CTCCCAGCCT ACCCAGGTGA CTCTGATAAC GGCCCCCAGC
    GGGGTGGAGG CCCAGCCTGT GCACGACCTC CCAGTGTCCA TTCTGGCCTC CCCTACTACA
    GAACAGCCCA CGGCCACGGT CACCATCGCT GATTCGGGCC AGGGTGATGT GCAGCCCGGC
    ACCGTGACCC TGGTGTGCTC CAACCCGCCG TGCGAAACCC ACGAGACGGG CACCACCAAT
    ACAGCCACCA CCACCGTCGT CGCTAATCTG GGGGGGCACC CCCAGCCCAC CCAAGTGCAG
    TTTGTCTGCG ACAGACAGGA GGCCACTGCT GCTCTCGTGA CCTCCACGGT GGGCCAGCCG
    AATGGCAGTG TAGTTCGTGT CTGCTCCAAC CCGCCGTGTG AAACCCACGA GACCGGCACC
    ACCAGTACAG CTACCACCGC CATGTCTGGC ATCGGAGGCG GGCCGCGGCG AGACATCCGG
    CTCGCCTGCG CGGCCACCAC CATTCCCACC GTGGTCCGGG TCAGCGTGAC TGCCGGGGCG
    TCAGAGGGAG CTCAGGTTTC CATCAAGCCC GCATGCCAAA CCCGTCAGAC CAGTGCAACC
    AGCACCACCA TGACTGTGAT GGCCACCGGG GCCCCGTGCT CGGCTGGCCC ACTCCTCAGA
    CCAAGCGTGG CCCTCGAGGC CGCTGGCCGC GGTGCCACTC TCTTGCAGCT GGGGCCACTG
    AGTGCCCAAG TCAGGCCCGG TGGCGAGGAA AGGTCCCTTG CCGGCCTGGG CCCGCTGGTG
    TCTGTGGGGC GCCAGCTGGA AGTGCATCAC ACGCACACGA CCAACACGCC CACTGTGGCC
    CGCTCCGCCA TGGGTGCCGG GGAGCCCCAC GAGCTGCTGG GGGCCCCCAC ACTTGTGTAC
    GAGAGCTCAG CCAGCGCCTC TGTGACTGCC GCAGCCCTGG AGGCACTGCT GTGCCCCTCG
    GCCGCCGTGA CCCAGGTCTG CTCCAACCCC CCGTGTGAGA CCCACGAGAC GGGCACCACC
    CACACACCCA CCACGGCCAC ATCCGGAGGG GCTGCAGGCC AGCCAGAGGG CGGGCAGCAG
    CCTCCTGCCA GCCGGCCCTG TGAGACGCAC CAGACCGCTT CCACTGGCAC GACCATGTCC
    GTCAGCTTGG GCGCCCTGCT CCCGGATGCC GCCCCCTCCC ACAGGACCCT GGAGTCCAGC
    CTGGAGGTGG CAGTGCCACC CGCAGTCGCC CCTCAGGCCG GTGCTTCGTT GCTCACTCCT
    TTCCCGACGC AAAGGGTGTG CTCCAACCCA CCCTGTGAGA CGCATGAGAC AGGCACTACG
    CACACGGCCA CCACTGTCAC CTCCAACATG AGTTCCAACC AAGACCCCCC ACCGCCTGCC
    GGCGACCAGG GAGAGGTGGA GAGCACCCAG GGCGACAGTG TGAATATCGC CAGTTCCAGT
    CCCATCACGA CAACAGTGTC CTCCACGCTG ACGCGGGCCG TGACCACCGT GACACAGTCC
    ACGCCAGTCC CAGGCCCTTC GGTGCCGAAG ATCTCATCTG TGACTGAGAC TGCCCCAGGG
    GCTCTGACCA CCGAAGTCCC CATCCCGGCC ACGATTACAG TGACCATAGC CAACACAGAA
    ACTTCTGACA TGCCCTTCTC TGCTGTTGAC ATCCTGCAGC CCCCAGAGGA GCTCCAGGCC
    TCTCCAGGGC CACGCCAGCA GCTTCCACCA CGGCAGCTCC TGCAGCCTGC CTCCGCGCCC
    TTGGTGGGGG ACTCCGCCGA GGTCCTGTCA GCCTCCCAGA GCCCTGAGCT CCAGGCCGCC
    GTGGATCTGA GCAGTACAGG GGACCCGTCT TCAGGCCAGG AGCCTGCCAG CTCGGCTGTG
    GTGGCCACCG TGGTGGTCCA GCCACCGCCG CCCACCCAGT CCGAAGTAGA CCAGTTGTCC
    CTCCCCCAAG AGCTGATGGC CGAAGCCCAG GCGGGCACCA CCACCCTCAT GGTGACAGGG
    CTCACCCCAG AGGAGCTGGC AGTGACTGCT GCTGCTGAAG CGGCCGCGCA GGCTGCAGCC
    ACAGAGGAGG CCCAGGCCCT GGCCATACAG GCCGTGCTCC AGGCCGCACA GCAGGCTGTC
    ATGGCAGGCA CCGGGGAGCC CATGGATACT TCAGAGGCGG CCGCAGCGGT GACACAGGCA
    GAGTTGAGCC ACCTGTCGGC CGAGGGCCAG GAAGGCCAGG CGACCACTAT CCCCATCGTG
    CTGACACAGC AGGAGCTGGC TGCCCTGGTC CAGCAGCAGC AGCTCCAGGA AGCACAGGCC
    CAGCAGCAGC AGCACCACCT GCCCACGGAA GCACTGGCCC CGGCTGACAG CCTCAATGAC
    CCGACCATCG AGAGCAACTG CCTCAATGAG CTGGCCGGGG CTGTGCCCAG CACCGTGAGC
    CTGCTGCCCC CCACGGCCAC TGAGAGCCTG GCCCCGTCCA ACACATTTGT GGCCCCCCAG
    CCGGTCGTTG TGGCCAGCCC CGCGAAGCTG CAGGCCGCAG CCACCCTGAC GGAAGTGGCC
    AATGGCATTG AGTCCCTGGG TGTGAAGCCA GACCTGCCAC CGCCACCTAG CAAAGCCCCT
    GTAAAGAAGG AGAACCAGTG GTTTGACGTG GGGGTCATTA AGGGTACCAA TGTGATGGTG
    ACACACTATT TCCTGCCACC CGAAGATGCT GTCCCGACTG ATGATGACTC GGGCACCGTG
    CCCGACTACA ACCAGCTAAA GAAGCAGGAG CTGCAGCCAG GCACTGCCTA TAAGTTCCGC
    GTCGCCGGGA TCAATGCCTG CGGCCGGGGG CCCTTCAGTG AGATCTCAGC CTTTAAAACG
    TGTCTGCCTG GCTTCCCAGG GGCCCCGTGT GCCATTAAAA TCAGCAAAAG TCCAGACGGT
    GCTCACCTCA CCTGGGAGCC ACCCTCTGTG ACCTCCGGCA AGATCATCGA GTACTCAGTG
    TACCTGGCCA TCCAGAGCGC GCAGGCCGGT GGTGAGACCA AGAGCTCGAC CCCGGCGCAG
    CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC AGCCCCTCCT GCCTCGTGCA GTCCTCCAGC
    CTCTCCAACG CCCACATCGA CTACACCACC AAGCCCGCCA TCATCTTCCG CATTGCTGCC
    CGCAATGAGA AGGGCTACGG CCCAGCCACC CAAGTCAGGT GGTTGCAAGA AACCAGTAAA
    GACAGCTCTG GCGCCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCC
    GCGCCAAAGA AATCGAAGGC AGACGGTCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317010.1
    CDS1371..7223
    Translation

    Target ORF information:

    RefSeq Version XM_015461524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGGAAT ACGGGAAGTA CAGCAACGAC CTCTACGAGC TCCAGGCAAG CCGGTGGGAA 
    TGGAAGAGAC TGAAAGCAAA GACGCCCAAA AATGGGCCAC CTCCGTGTCC TCGGCTTGGG
    CACAGCTTCT CCCTTGTGGG CAACAAGTGT TACCTGTTTG GGGGTCTGGC CAATGATAGC
    GAGGACCCCA AGAATAACAT TCCGAGGTAC CTGAATGACT TATACATCCT GGAACTGCGG
    CCGGGCTCTG GAGTGGTAGC CTGGGACATT CCCATCACTT ACGGCGTCCT GCCCCCACCC
    CGAGAGTCTC ACACTGCAGT GGTCTACACT GAGAAAGACA ACAAGAAGTC CAAACTGGTG
    ATCTATGGAG GGATGAGTGG CTGCAGGCTG GGGGACCTCT GGACCTTGGA TATCGAGACT
    TTGACATGGA ATAAGCCCAG CCTCAGCGGG GTGGCACCTC TTCCTCGAAG TCTCCACTCG
    GCCACGACCA TAGGAAACAA AATGTACGTG TTTGGTGGCT GGGTGCCTCT CGTCATGGAT
    GACGTCAAAG TGGCCACACA CGAGAAGGAG TGGAAGTGTA CCAACACTCT GGCTTGTCTC
    AACCTGGATA CCATGGCTTG GGAGACCATC CTGATGGATA CGCTGGAAGA CAATATTCCC
    CGGGCCCGTG CTGGCCACTG TGCGGTAGCC ATCAATACCC GCCTTTACAT TTGGAGTGGG
    CGTGACGGCT ACCGAAAGGC CTGGAATAAC CAGGTCTGCT GCAAGGACCT CTGGTACCTG
    GAAACGGAGA AGCCGCCACC TCCAGCCCGG GTACAGCTGG TGCGAGCCAA CACTAACTCC
    CTGGAGGTGA GCTGGGGGGC CGTGGCAACA GCCGACAGTT ACCTTCTGCA GCTCCAGAAA
    TATGACATTC CTGCCACGGC TGCCACTGCC ACCTCCCCCA CACCCAATCC AGTCCCGTCT
    GTGCCTGCCA ATCCTCCCAA GAGCCCTGCC CCAGCAGCAG CCGCACCTGC CGTGCAGCCG
    CTGACCCAAG TAGGCATCAC GCTCCTGCCC CAGGCTGCTG CCGCGCCCCC GACCACCACC
    ACCATCCAGG TCTTGCCGAC GGTGCCTGGC AGCTCAATCT CCGTGCCCGC CGCAGCCAGG
    ACTCAAGGTG TCCCGGCTGT GCTGAAAGTG ACTGGTCCTC AGGCTACCAC GGGAACCCCG
    TTGGTCACCA TGCGACCTGC CAGCCAGGCT GGGAAAGCCC CCGTGACTGT GACCTCCCTT
    CCCGCAGGCG TGCGGATGGT TGTGCCTACA CAGAGCGCCC AGGGGACAGT CATTGGCAGC
    AGCCCGCAGA TGAGTGGCAT GGCTGCATTG GCAGCCGCGG CTGCTGCCAC CCAGAAGATC
    CCTCCTTCCT CGGCACCCAC GGTCCTGAGT GTCCCGGCTG GCACCACTAT CGTCAAAACC
    GTGGCCGTGA CGCCGGGCAC CACCACGCTC CCAGCCACTG TGAAGGTGGC CTCCTCGCCA
    GTCATGGTGA GCAACCCAGC CACTCGCATG CTGAAGACTG CAGCTGCCCA GGTGGGGACT
    TCCGTCTCTT CTGCCGCCAA CACATCTACC CGTCCCATCA TCACCGTACA CAAGTCCGGA
    ACCGTGACAG TGGCCCAGCA AGCCCAGGTG GTGACCACAG TGGTGGGTGG GGTTACCAAG
    ACCATCACCC TGGTGAAGAG CCCCATCTCG GTCCCAGGAG GCAGTGCTCT GATTTCCAAT
    CTGGGCAAAG TAATGTCAGT GGTCCAGACC AAACCGGTTC AGACTTCGGC AGTCACAGGC
    CAGGCATCTA CAGGCCCGGT GACTCAGATC ATCCAGACCA AAGGACCCTT GCCAGCCGGG
    ACCATCCTGA AGCTGGTGAC GTCTGCAGAT GGCAAGCCCA CTACCATCAT CACAACCACA
    CAGGCCAGCG GGGCAGGGAC TAAGCCTACC ATCCTGGGCA TCAGCAGTGT GTCCCCCAGC
    ACCACCAAGC CCGGCACCAC CACTATCATT AAGACCATCC CCATGTCGGC CATCATCACA
    CAGGCGGGCG CCACGGGTAG GGGCCTTCCC CGGTGTGCTG GGGAGAAAGC AGGCGTGACT
    AGCAGTGCCG GCATCAAGTC ACCCATCACC ATTATCACCA CCAAGGTGAT GACTTCCGGA
    ACTGGAGCCC CCGCCAAAAT CATCACGGCT GTTCCCAAAA TCGCTACGGG TCACGGGCAG
    CAAGGAGTGA CCCAGGTGGT GCTGAAGGGC GCACCTGGCC AGCCAGGCAC CATCCTCCGC
    ACCGTGCCTA TGGGGGGCGT CCGCCTGGTC ACCCCTGTTA CCGTCTCTGC TGTCAAGCCT
    GCTGTCACCA CACTGGTTGT CAAGGGCACC ACAGGCGTCA CGACCCTGGG CACGGTGACA
    GGCACCGTCT CCACCAGCCT GGCCGGAGCC GGGGGCCATA GCACCAGCGC CTCACTGGCC
    ACACCCATCA CCACGTTGGG CACCATCGCC ACCCTCTCAA GCCAGGTGAT CAACCCCACT
    GCCATCACTG TGTCTGCGGC GCAGACGACG CTGACAGCGG CCGCTGGGCT TACCACACCC
    ACCATAACCA TGCAGCCTGT CTCCCAGCCT ACCCAGGTGA CTCTGATAAC GGCCCCCAGC
    GGGGTGGAGG CCCAGCCTGT GCACGACCTC CCAGTGTCCA TTCTGGCCTC CCCTACTACA
    GAACAGCCCA CGGCCACGGT CACCATCGCT GATTCGGGCC AGGGTGATGT GCAGCCCGGC
    ACCGTGACCC TGGTGTGCTC CAACCCGCCG TGCGAAACCC ACGAGACGGG CACCACCAAT
    ACAGCCACCA CCACCGTCGT CGCTAATCTG GGGGGGCACC CCCAGCCCAC CCAAGTGCAG
    TTTGTCTGCG ACAGACAGGA GGCCACTGCT GCTCTCGTGA CCTCCACGGT GGGCCAGCCG
    AATGGCAGTG TAGTTCGTGT CTGCTCCAAC CCGCCGTGTG AAACCCACGA GACCGGCACC
    ACCAGTACAG CTACCACCGC CATGTCTGGC ATCGGAGGCG GGCCGCGGCG AGACATCCGG
    CTCGCCTGCG CGGCCACCAC CATTCCCACC GTGGTCCGGG TCAGCGTGAC TGCCGGGGCG
    TCAGAGGGAG CTCAGGTTTC CATCAAGCCC GCATGCCAAA CCCGTCAGAC CAGTGCAACC
    AGCACCACCA TGACTGTGAT GGCCACCGGG GCCCCGTGCT CGGCTGGCCC ACTCCTCAGA
    CCAAGCGTGG CCCTCGAGGC CGCTGGCCGC GGTGCCACTC TCTTGCAGCT GGGGCCACTG
    AGTGCCCAAG TCAGGCCCGG TGGCGAGGAA AGGTCCCTTG CCGGCCTGGG CCCGCTGGTG
    TCTGTGGGGC GCCAGCTGGA AGTGCATCAC ACGCACACGA CCAACACGCC CACTGTGGCC
    CGCTCCGCCA TGGGTGCCGG GGAGCCCCAC GAGCTGCTGG GGGCCCCCAC ACTTGTGTAC
    GAGAGCTCAG CCAGCGCCTC TGTGACTGCC GCAGCCCTGG AGGCACTGCT GTGCCCCTCG
    GCCGCCGTGA CCCAGGTCTG CTCCAACCCC CCGTGTGAGA CCCACGAGAC GGGCACCACC
    CACACACCCA CCACGGCCAC ATCCGGAGGG GCTGCAGGCC AGCCAGAGGG CGGGCAGCAG
    CCTCCTGCCA GCCGGCCCTG TGAGACGCAC CAGACCGCTT CCACTGGCAC GACCATGTCC
    GTCAGCTTGG GCGCCCTGCT CCCGGATGCC GCCCCCTCCC ACAGGACCCT GGAGTCCAGC
    CTGGAGGTGG CAGTGCCACC CGCAGTCGCC CCTCAGGCCG GTGCTTCGTT GCTCACTCCT
    TTCCCGACGC AAAGGGTGTG CTCCAACCCA CCCTGTGAGA CGCATGAGAC AGGCACTACG
    CACACGGCCA CCACTGTCAC CTCCAACATG AGTTCCAACC AAGACCCCCC ACCGCCTGCC
    GGCGACCAGG GAGAGGTGGA GAGCACCCAG GGCGACAGTG TGAATATCGC CAGTTCCAGT
    CCCATCACGA CAACAGTGTC CTCCACGCTG ACGCGGGCCG TGACCACCGT GACACAGTCC
    ACGCCAGTCC CAGGCCCTTC GGTGCCGAAG ATCTCATCTG TGACTGAGAC TGCCCCAGGG
    GCTCTGACCA CCGAAGTCCC CATCCCGGCC ACGATTACAG TGACCATAGC CAACACAGAA
    ACTTCTGACA TGCCCTTCTC TGCTGTTGAC ATCCTGCAGC CCCCAGAGGA GCTCCAGGCC
    TCTCCAGGGC CACGCCAGCA GCTTCCACCA CGGCAGCTCC TGCAGCCTGC CTCCGCGCCC
    TTGGTGGGGG ACTCCGCCGA GGTCCTGTCA GCCTCCCAGA GCCCTGAGCT CCAGGCCGCC
    GTGGATCTGA GCAGTACAGG GGACCCGTCT TCAGGCCAGG AGCCTGCCAG CTCGGCTGTG
    GTGGCCACCG TGGTGGTCCA GCCACCGCCG CCCACCCAGT CCGAAGTAGA CCAGTTGTCC
    CTCCCCCAAG AGCTGATGGC CGAAGCCCAG GCGGGCACCA CCACCCTCAT GGTGACAGGG
    CTCACCCCAG AGGAGCTGGC AGTGACTGCT GCTGCTGAAG CGGCCGCGCA GGCTGCAGCC
    ACAGAGGAGG CCCAGGCCCT GGCCATACAG GCCGTGCTCC AGGCCGCACA GCAGGCTGTC
    ATGGCAGGCA CCGGGGAGCC CATGGATACT TCAGAGGCGG CCGCAGCGGT GACACAGGCA
    GAGTTGAGCC ACCTGTCGGC CGAGGGCCAG GAAGGCCAGG CGACCACTAT CCCCATCGTG
    CTGACACAGC AGGAGCTGGC TGCCCTGGTC CAGCAGCAGC AGCTCCAGGA AGCACAGGCC
    CAGCAGCAGC AGCACCACCT GCCCACGGAA GCACTGGCCC CGGCTGACAG CCTCAATGAC
    CCGACCATCG AGAGCAACTG CCTCAATGAG CTGGCCGGGG CTGTGCCCAG CACCGTGAGC
    CTGCTGCCCC CCACGGCCAC TGAGAGCCTG GCCCCGTCCA ACACATTTGT GGCCCCCCAG
    CCGGTCGTTG TGGCCAGCCC CGCGAAGCTG CAGGCCGCAG CCACCCTGAC GGAAGTGGCC
    AATGGCATTG AGTCCCTGGG TGTGAAGCCA GACCTGCCAC CGCCACCTAG CAAAGCCCCT
    GTAAAGAAGG AGAACCAGTG GTTTGACGTG GGGGTCATTA AGGGTACCAA TGTGATGGTG
    ACACACTATT TCCTGCCACC CGAAGATGCT GTCCCGACTG ATGATGACTC GGGCACCGTG
    CCCGACTACA ACCAGCTAAA GAAGCAGGAG CTGCAGCCAG GCACTGCCTA TAAGTTCCGC
    GTCGCCGGGA TCAATGCCTG CGGCCGGGGG CCCTTCAGTG AGATCTCAGC CTTTAAAACG
    TGTCTGCCTG GCTTCCCAGG GGCCCCGTGT GCCATTAAAA TCAGCAAAAG TCCAGACGGT
    GCTCACCTCA CCTGGGAGCC ACCCTCTGTG ACCTCCGGCA AGATCATCGA GTACTCAGTG
    TACCTGGCCA TCCAGAGCGC GCAGGCCGGT GGTGAGACCA AGAGCTCGAC CCCGGCGCAG
    CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC AGCCCCTCCT GCCTCGTGCA GTCCTCCAGC
    CTCTCCAACG CCCACATCGA CTACACCACC AAGCCCGCCA TCATCTTCCG CATTGCTGCC
    CGCAATGAGA AGGGCTACGG CCCAGCCACC CAAGTCAGGT GGTTGCAAGA AACCAGTAAA
    GACAGCTCTG GCGCCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCC
    GCGCCAAAGA AATCGAAGGC AGACGGTCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89281
    Clone ID Related Accession (Same CDS sequence) XM_015461525.1 , XM_015470290.1
    Accession Version XM_015461525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTTGGG AGACCATCCT GATGGATACG CTGGAAGACA ATATTCCCCG GGCCCGTGCT 
    GGCCACTGTG CGGTAGCCAT CAATACCCGC CTTTACATTT GGAGTGGGCG TGACGGCTAC
    CGAAAGGCCT GGAATAACCA GGTCTGCTGC AAGGACCTCT GGTACCTGGA AACGGAGAAG
    CCGCCACCTC CAGCCCGGGT ACAGCTGGTG CGAGCCAACA CTAACTCCCT GGAGGTGAGC
    TGGGGGGCCG TGGCAACAGC CGACAGTTAC CTTCTGCAGC TCCAGAAATA TGACATTCCT
    GCCACGGCTG CCACTGCCAC CTCCCCCACA CCCAATCCAG TCCCGTCTGT GCCTGCCAAT
    CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCCG TGCAGCCGCT GACCCAAGTA
    GGCATCACGC TCCTGCCCCA GGCTGCTGCC GCGCCCCCGA CCACCACCAC CATCCAGGTC
    TTGCCGACGG TGCCTGGCAG CTCAATCTCC GTGCCCGCCG CAGCCAGGAC TCAAGGTGTC
    CCGGCTGTGC TGAAAGTGAC TGGTCCTCAG GCTACCACGG GAACCCCGTT GGTCACCATG
    CGACCTGCCA GCCAGGCTGG GAAAGCCCCC GTGACTGTGA CCTCCCTTCC CGCAGGCGTG
    CGGATGGTTG TGCCTACACA GAGCGCCCAG GGGACAGTCA TTGGCAGCAG CCCGCAGATG
    AGTGGCATGG CTGCATTGGC AGCCGCGGCT GCTGCCACCC AGAAGATCCC TCCTTCCTCG
    GCACCCACGG TCCTGAGTGT CCCGGCTGGC ACCACTATCG TCAAAACCGT GGCCGTGACG
    CCGGGCACCA CCACGCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC
    AACCCAGCCA CTCGCATGCT GAAGACTGCA GCTGCCCAGG TGGGGACTTC CGTCTCTTCT
    GCCGCCAACA CATCTACCCG TCCCATCATC ACCGTACACA AGTCCGGAAC CGTGACAGTG
    GCCCAGCAAG CCCAGGTGGT GACCACAGTG GTGGGTGGGG TTACCAAGAC CATCACCCTG
    GTGAAGAGCC CCATCTCGGT CCCAGGAGGC AGTGCTCTGA TTTCCAATCT GGGCAAAGTA
    ATGTCAGTGG TCCAGACCAA ACCGGTTCAG ACTTCGGCAG TCACAGGCCA GGCATCTACA
    GGCCCGGTGA CTCAGATCAT CCAGACCAAA GGACCCTTGC CAGCCGGGAC CATCCTGAAG
    CTGGTGACGT CTGCAGATGG CAAGCCCACT ACCATCATCA CAACCACACA GGCCAGCGGG
    GCAGGGACTA AGCCTACCAT CCTGGGCATC AGCAGTGTGT CCCCCAGCAC CACCAAGCCC
    GGCACCACCA CTATCATTAA GACCATCCCC ATGTCGGCCA TCATCACACA GGCGGGCGCC
    ACGGGTAGGG GCCTTCCCCG GTGTGCTGGG GAGAAAGCAG GCGTGACTAG CAGTGCCGGC
    ATCAAGTCAC CCATCACCAT TATCACCACC AAGGTGATGA CTTCCGGAAC TGGAGCCCCC
    GCCAAAATCA TCACGGCTGT TCCCAAAATC GCTACGGGTC ACGGGCAGCA AGGAGTGACC
    CAGGTGGTGC TGAAGGGCGC ACCTGGCCAG CCAGGCACCA TCCTCCGCAC CGTGCCTATG
    GGGGGCGTCC GCCTGGTCAC CCCTGTTACC GTCTCTGCTG TCAAGCCTGC TGTCACCACA
    CTGGTTGTCA AGGGCACCAC AGGCGTCACG ACCCTGGGCA CGGTGACAGG CACCGTCTCC
    ACCAGCCTGG CCGGAGCCGG GGGCCATAGC ACCAGCGCCT CACTGGCCAC ACCCATCACC
    ACGTTGGGCA CCATCGCCAC CCTCTCAAGC CAGGTGATCA ACCCCACTGC CATCACTGTG
    TCTGCGGCGC AGACGACGCT GACAGCGGCC GCTGGGCTTA CCACACCCAC CATAACCATG
    CAGCCTGTCT CCCAGCCTAC CCAGGTGACT CTGATAACGG CCCCCAGCGG GGTGGAGGCC
    CAGCCTGTGC ACGACCTCCC AGTGTCCATT CTGGCCTCCC CTACTACAGA ACAGCCCACG
    GCCACGGTCA CCATCGCTGA TTCGGGCCAG GGTGATGTGC AGCCCGGCAC CGTGACCCTG
    GTGTGCTCCA ACCCGCCGTG CGAAACCCAC GAGACGGGCA CCACCAATAC AGCCACCACC
    ACCGTCGTCG CTAATCTGGG GGGGCACCCC CAGCCCACCC AAGTGCAGTT TGTCTGCGAC
    AGACAGGAGG CCACTGCTGC TCTCGTGACC TCCACGGTGG GCCAGCCGAA TGGCAGTGTA
    GTTCGTGTCT GCTCCAACCC GCCGTGTGAA ACCCACGAGA CCGGCACCAC CAGTACAGCT
    ACCACCGCCA TGTCTGGCAT CGGAGGCGGG CCGCGGCGAG ACATCCGGCT CGCCTGCGCG
    GCCACCACCA TTCCCACCGT GGTCCGGGTC AGCGTGACTG CCGGGGCGTC AGAGGGAGCT
    CAGGTTTCCA TCAAGCCCGC ATGCCAAACC CGTCAGACCA GTGCAACCAG CACCACCATG
    ACTGTGATGG CCACCGGGGC CCCGTGCTCG GCTGGCCCAC TCCTCAGACC AAGCGTGGCC
    CTCGAGGCCG CTGGCCGCGG TGCCACTCTC TTGCAGCTGG GGCCACTGAG TGCCCAAGTC
    AGGCCCGGTG GCGAGGAAAG GTCCCTTGCC GGCCTGGGCC CGCTGGTGTC TGTGGGGCGC
    CAGCTGGAAG TGCATCACAC GCACACGACC AACACGCCCA CTGTGGCCCG CTCCGCCATG
    GGTGCCGGGG AGCCCCACGA GCTGCTGGGG GCCCCCACAC TTGTGTACGA GAGCTCAGCC
    AGCGCCTCTG TGACTGCCGC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CGCCGTGACC
    CAGGTCTGCT CCAACCCCCC GTGTGAGACC CACGAGACGG GCACCACCCA CACACCCACC
    ACGGCCACAT CCGGAGGGGC TGCAGGCCAG CCAGAGGGCG GGCAGCAGCC TCCTGCCAGC
    CGGCCCTGTG AGACGCACCA GACCGCTTCC ACTGGCACGA CCATGTCCGT CAGCTTGGGC
    GCCCTGCTCC CGGATGCCGC CCCCTCCCAC AGGACCCTGG AGTCCAGCCT GGAGGTGGCA
    GTGCCACCCG CAGTCGCCCC TCAGGCCGGT GCTTCGTTGC TCACTCCTTT CCCGACGCAA
    AGGGTGTGCT CCAACCCACC CTGTGAGACG CATGAGACAG GCACTACGCA CACGGCCACC
    ACTGTCACCT CCAACATGAG TTCCAACCAA GACCCCCCAC CGCCTGCCGG CGACCAGGGA
    GAGGTGGAGA GCACCCAGGG CGACAGTGTG AATATCGCCA GTTCCAGTCC CATCACGACA
    ACAGTGTCCT CCACGCTGAC GCGGGCCGTG ACCACCGTGA CACAGTCCAC GCCAGTCCCA
    GGCCCTTCGG TGCCGAAGAT CTCATCTGTG ACTGAGACTG CCCCAGGGGC TCTGACCACC
    GAAGTCCCCA TCCCGGCCAC GATTACAGTG ACCATAGCCA ACACAGAAAC TTCTGACATG
    CCCTTCTCTG CTGTTGACAT CCTGCAGCCC CCAGAGGAGC TCCAGGCCTC TCCAGGGCCA
    CGCCAGCAGC TTCCACCACG GCAGCTCCTG CAGCCTGCCT CCGCGCCCTT GGTGGGGGAC
    TCCGCCGAGG TCCTGTCAGC CTCCCAGAGC CCTGAGCTCC AGGCCGCCGT GGATCTGAGC
    AGTACAGGGG ACCCGTCTTC AGGCCAGGAG CCTGCCAGCT CGGCTGTGGT GGCCACCGTG
    GTGGTCCAGC CACCGCCGCC CACCCAGTCC GAAGTAGACC AGTTGTCCCT CCCCCAAGAG
    CTGATGGCCG AAGCCCAGGC GGGCACCACC ACCCTCATGG TGACAGGGCT CACCCCAGAG
    GAGCTGGCAG TGACTGCTGC TGCTGAAGCG GCCGCGCAGG CTGCAGCCAC AGAGGAGGCC
    CAGGCCCTGG CCATACAGGC CGTGCTCCAG GCCGCACAGC AGGCTGTCAT GGCAGGCACC
    GGGGAGCCCA TGGATACTTC AGAGGCGGCC GCAGCGGTGA CACAGGCAGA GTTGAGCCAC
    CTGTCGGCCG AGGGCCAGGA AGGCCAGGCG ACCACTATCC CCATCGTGCT GACACAGCAG
    GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG CTCCAGGAAG CACAGGCCCA GCAGCAGCAG
    CACCACCTGC CCACGGAAGC ACTGGCCCCG GCTGACAGCC TCAATGACCC GACCATCGAG
    AGCAACTGCC TCAATGAGCT GGCCGGGGCT GTGCCCAGCA CCGTGAGCCT GCTGCCCCCC
    ACGGCCACTG AGAGCCTGGC CCCGTCCAAC ACATTTGTGG CCCCCCAGCC GGTCGTTGTG
    GCCAGCCCCG CGAAGCTGCA GGCCGCAGCC ACCCTGACGG AAGTGGCCAA TGGCATTGAG
    TCCCTGGGTG TGAAGCCAGA CCTGCCACCG CCACCTAGCA AAGCCCCTGT AAAGAAGGAG
    AACCAGTGGT TTGACGTGGG GGTCATTAAG GGTACCAATG TGATGGTGAC ACACTATTTC
    CTGCCACCCG AAGATGCTGT CCCGACTGAT GATGACTCGG GCACCGTGCC CGACTACAAC
    CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC ACTGCCTATA AGTTCCGCGT CGCCGGGATC
    AATGCCTGCG GCCGGGGGCC CTTCAGTGAG ATCTCAGCCT TTAAAACGTG TCTGCCTGGC
    TTCCCAGGGG CCCCGTGTGC CATTAAAATC AGCAAAAGTC CAGACGGTGC TCACCTCACC
    TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCAGTGTA CCTGGCCATC
    CAGAGCGCGC AGGCCGGTGG TGAGACCAAG AGCTCGACCC CGGCGCAGCT GGCCTTCATG
    CGGGTGTACT GCGGGCCCAG CCCCTCCTGC CTCGTGCAGT CCTCCAGCCT CTCCAACGCC
    CACATCGACT ACACCACCAA GCCCGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAGAAG
    GGCTACGGCC CAGCCACCCA AGTCAGGTGG TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC
    GCCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCCGC GCCAAAGAAA
    TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317011.1
    CDS78..5318
    Translation

    Target ORF information:

    RefSeq Version XM_015461525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTTGGG AGACCATCCT GATGGATACG CTGGAAGACA ATATTCCCCG GGCCCGTGCT 
    GGCCACTGTG CGGTAGCCAT CAATACCCGC CTTTACATTT GGAGTGGGCG TGACGGCTAC
    CGAAAGGCCT GGAATAACCA GGTCTGCTGC AAGGACCTCT GGTACCTGGA AACGGAGAAG
    CCGCCACCTC CAGCCCGGGT ACAGCTGGTG CGAGCCAACA CTAACTCCCT GGAGGTGAGC
    TGGGGGGCCG TGGCAACAGC CGACAGTTAC CTTCTGCAGC TCCAGAAATA TGACATTCCT
    GCCACGGCTG CCACTGCCAC CTCCCCCACA CCCAATCCAG TCCCGTCTGT GCCTGCCAAT
    CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCCG TGCAGCCGCT GACCCAAGTA
    GGCATCACGC TCCTGCCCCA GGCTGCTGCC GCGCCCCCGA CCACCACCAC CATCCAGGTC
    TTGCCGACGG TGCCTGGCAG CTCAATCTCC GTGCCCGCCG CAGCCAGGAC TCAAGGTGTC
    CCGGCTGTGC TGAAAGTGAC TGGTCCTCAG GCTACCACGG GAACCCCGTT GGTCACCATG
    CGACCTGCCA GCCAGGCTGG GAAAGCCCCC GTGACTGTGA CCTCCCTTCC CGCAGGCGTG
    CGGATGGTTG TGCCTACACA GAGCGCCCAG GGGACAGTCA TTGGCAGCAG CCCGCAGATG
    AGTGGCATGG CTGCATTGGC AGCCGCGGCT GCTGCCACCC AGAAGATCCC TCCTTCCTCG
    GCACCCACGG TCCTGAGTGT CCCGGCTGGC ACCACTATCG TCAAAACCGT GGCCGTGACG
    CCGGGCACCA CCACGCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC
    AACCCAGCCA CTCGCATGCT GAAGACTGCA GCTGCCCAGG TGGGGACTTC CGTCTCTTCT
    GCCGCCAACA CATCTACCCG TCCCATCATC ACCGTACACA AGTCCGGAAC CGTGACAGTG
    GCCCAGCAAG CCCAGGTGGT GACCACAGTG GTGGGTGGGG TTACCAAGAC CATCACCCTG
    GTGAAGAGCC CCATCTCGGT CCCAGGAGGC AGTGCTCTGA TTTCCAATCT GGGCAAAGTA
    ATGTCAGTGG TCCAGACCAA ACCGGTTCAG ACTTCGGCAG TCACAGGCCA GGCATCTACA
    GGCCCGGTGA CTCAGATCAT CCAGACCAAA GGACCCTTGC CAGCCGGGAC CATCCTGAAG
    CTGGTGACGT CTGCAGATGG CAAGCCCACT ACCATCATCA CAACCACACA GGCCAGCGGG
    GCAGGGACTA AGCCTACCAT CCTGGGCATC AGCAGTGTGT CCCCCAGCAC CACCAAGCCC
    GGCACCACCA CTATCATTAA GACCATCCCC ATGTCGGCCA TCATCACACA GGCGGGCGCC
    ACGGGTAGGG GCCTTCCCCG GTGTGCTGGG GAGAAAGCAG GCGTGACTAG CAGTGCCGGC
    ATCAAGTCAC CCATCACCAT TATCACCACC AAGGTGATGA CTTCCGGAAC TGGAGCCCCC
    GCCAAAATCA TCACGGCTGT TCCCAAAATC GCTACGGGTC ACGGGCAGCA AGGAGTGACC
    CAGGTGGTGC TGAAGGGCGC ACCTGGCCAG CCAGGCACCA TCCTCCGCAC CGTGCCTATG
    GGGGGCGTCC GCCTGGTCAC CCCTGTTACC GTCTCTGCTG TCAAGCCTGC TGTCACCACA
    CTGGTTGTCA AGGGCACCAC AGGCGTCACG ACCCTGGGCA CGGTGACAGG CACCGTCTCC
    ACCAGCCTGG CCGGAGCCGG GGGCCATAGC ACCAGCGCCT CACTGGCCAC ACCCATCACC
    ACGTTGGGCA CCATCGCCAC CCTCTCAAGC CAGGTGATCA ACCCCACTGC CATCACTGTG
    TCTGCGGCGC AGACGACGCT GACAGCGGCC GCTGGGCTTA CCACACCCAC CATAACCATG
    CAGCCTGTCT CCCAGCCTAC CCAGGTGACT CTGATAACGG CCCCCAGCGG GGTGGAGGCC
    CAGCCTGTGC ACGACCTCCC AGTGTCCATT CTGGCCTCCC CTACTACAGA ACAGCCCACG
    GCCACGGTCA CCATCGCTGA TTCGGGCCAG GGTGATGTGC AGCCCGGCAC CGTGACCCTG
    GTGTGCTCCA ACCCGCCGTG CGAAACCCAC GAGACGGGCA CCACCAATAC AGCCACCACC
    ACCGTCGTCG CTAATCTGGG GGGGCACCCC CAGCCCACCC AAGTGCAGTT TGTCTGCGAC
    AGACAGGAGG CCACTGCTGC TCTCGTGACC TCCACGGTGG GCCAGCCGAA TGGCAGTGTA
    GTTCGTGTCT GCTCCAACCC GCCGTGTGAA ACCCACGAGA CCGGCACCAC CAGTACAGCT
    ACCACCGCCA TGTCTGGCAT CGGAGGCGGG CCGCGGCGAG ACATCCGGCT CGCCTGCGCG
    GCCACCACCA TTCCCACCGT GGTCCGGGTC AGCGTGACTG CCGGGGCGTC AGAGGGAGCT
    CAGGTTTCCA TCAAGCCCGC ATGCCAAACC CGTCAGACCA GTGCAACCAG CACCACCATG
    ACTGTGATGG CCACCGGGGC CCCGTGCTCG GCTGGCCCAC TCCTCAGACC AAGCGTGGCC
    CTCGAGGCCG CTGGCCGCGG TGCCACTCTC TTGCAGCTGG GGCCACTGAG TGCCCAAGTC
    AGGCCCGGTG GCGAGGAAAG GTCCCTTGCC GGCCTGGGCC CGCTGGTGTC TGTGGGGCGC
    CAGCTGGAAG TGCATCACAC GCACACGACC AACACGCCCA CTGTGGCCCG CTCCGCCATG
    GGTGCCGGGG AGCCCCACGA GCTGCTGGGG GCCCCCACAC TTGTGTACGA GAGCTCAGCC
    AGCGCCTCTG TGACTGCCGC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CGCCGTGACC
    CAGGTCTGCT CCAACCCCCC GTGTGAGACC CACGAGACGG GCACCACCCA CACACCCACC
    ACGGCCACAT CCGGAGGGGC TGCAGGCCAG CCAGAGGGCG GGCAGCAGCC TCCTGCCAGC
    CGGCCCTGTG AGACGCACCA GACCGCTTCC ACTGGCACGA CCATGTCCGT CAGCTTGGGC
    GCCCTGCTCC CGGATGCCGC CCCCTCCCAC AGGACCCTGG AGTCCAGCCT GGAGGTGGCA
    GTGCCACCCG CAGTCGCCCC TCAGGCCGGT GCTTCGTTGC TCACTCCTTT CCCGACGCAA
    AGGGTGTGCT CCAACCCACC CTGTGAGACG CATGAGACAG GCACTACGCA CACGGCCACC
    ACTGTCACCT CCAACATGAG TTCCAACCAA GACCCCCCAC CGCCTGCCGG CGACCAGGGA
    GAGGTGGAGA GCACCCAGGG CGACAGTGTG AATATCGCCA GTTCCAGTCC CATCACGACA
    ACAGTGTCCT CCACGCTGAC GCGGGCCGTG ACCACCGTGA CACAGTCCAC GCCAGTCCCA
    GGCCCTTCGG TGCCGAAGAT CTCATCTGTG ACTGAGACTG CCCCAGGGGC TCTGACCACC
    GAAGTCCCCA TCCCGGCCAC GATTACAGTG ACCATAGCCA ACACAGAAAC TTCTGACATG
    CCCTTCTCTG CTGTTGACAT CCTGCAGCCC CCAGAGGAGC TCCAGGCCTC TCCAGGGCCA
    CGCCAGCAGC TTCCACCACG GCAGCTCCTG CAGCCTGCCT CCGCGCCCTT GGTGGGGGAC
    TCCGCCGAGG TCCTGTCAGC CTCCCAGAGC CCTGAGCTCC AGGCCGCCGT GGATCTGAGC
    AGTACAGGGG ACCCGTCTTC AGGCCAGGAG CCTGCCAGCT CGGCTGTGGT GGCCACCGTG
    GTGGTCCAGC CACCGCCGCC CACCCAGTCC GAAGTAGACC AGTTGTCCCT CCCCCAAGAG
    CTGATGGCCG AAGCCCAGGC GGGCACCACC ACCCTCATGG TGACAGGGCT CACCCCAGAG
    GAGCTGGCAG TGACTGCTGC TGCTGAAGCG GCCGCGCAGG CTGCAGCCAC AGAGGAGGCC
    CAGGCCCTGG CCATACAGGC CGTGCTCCAG GCCGCACAGC AGGCTGTCAT GGCAGGCACC
    GGGGAGCCCA TGGATACTTC AGAGGCGGCC GCAGCGGTGA CACAGGCAGA GTTGAGCCAC
    CTGTCGGCCG AGGGCCAGGA AGGCCAGGCG ACCACTATCC CCATCGTGCT GACACAGCAG
    GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG CTCCAGGAAG CACAGGCCCA GCAGCAGCAG
    CACCACCTGC CCACGGAAGC ACTGGCCCCG GCTGACAGCC TCAATGACCC GACCATCGAG
    AGCAACTGCC TCAATGAGCT GGCCGGGGCT GTGCCCAGCA CCGTGAGCCT GCTGCCCCCC
    ACGGCCACTG AGAGCCTGGC CCCGTCCAAC ACATTTGTGG CCCCCCAGCC GGTCGTTGTG
    GCCAGCCCCG CGAAGCTGCA GGCCGCAGCC ACCCTGACGG AAGTGGCCAA TGGCATTGAG
    TCCCTGGGTG TGAAGCCAGA CCTGCCACCG CCACCTAGCA AAGCCCCTGT AAAGAAGGAG
    AACCAGTGGT TTGACGTGGG GGTCATTAAG GGTACCAATG TGATGGTGAC ACACTATTTC
    CTGCCACCCG AAGATGCTGT CCCGACTGAT GATGACTCGG GCACCGTGCC CGACTACAAC
    CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC ACTGCCTATA AGTTCCGCGT CGCCGGGATC
    AATGCCTGCG GCCGGGGGCC CTTCAGTGAG ATCTCAGCCT TTAAAACGTG TCTGCCTGGC
    TTCCCAGGGG CCCCGTGTGC CATTAAAATC AGCAAAAGTC CAGACGGTGC TCACCTCACC
    TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCAGTGTA CCTGGCCATC
    CAGAGCGCGC AGGCCGGTGG TGAGACCAAG AGCTCGACCC CGGCGCAGCT GGCCTTCATG
    CGGGTGTACT GCGGGCCCAG CCCCTCCTGC CTCGTGCAGT CCTCCAGCCT CTCCAACGCC
    CACATCGACT ACACCACCAA GCCCGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAGAAG
    GGCTACGGCC CAGCCACCCA AGTCAGGTGG TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC
    GCCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCCGC GCCAAAGAAA
    TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89281
    Clone ID Related Accession (Same CDS sequence) XM_015461525.1 , XM_015470290.1
    Accession Version XM_015470290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product host cell factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647006.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTTGGG AGACCATCCT GATGGATACG CTGGAAGACA ATATTCCCCG GGCCCGTGCT 
    GGCCACTGTG CGGTAGCCAT CAATACCCGC CTTTACATTT GGAGTGGGCG TGACGGCTAC
    CGAAAGGCCT GGAATAACCA GGTCTGCTGC AAGGACCTCT GGTACCTGGA AACGGAGAAG
    CCGCCACCTC CAGCCCGGGT ACAGCTGGTG CGAGCCAACA CTAACTCCCT GGAGGTGAGC
    TGGGGGGCCG TGGCAACAGC CGACAGTTAC CTTCTGCAGC TCCAGAAATA TGACATTCCT
    GCCACGGCTG CCACTGCCAC CTCCCCCACA CCCAATCCAG TCCCGTCTGT GCCTGCCAAT
    CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCCG TGCAGCCGCT GACCCAAGTA
    GGCATCACGC TCCTGCCCCA GGCTGCTGCC GCGCCCCCGA CCACCACCAC CATCCAGGTC
    TTGCCGACGG TGCCTGGCAG CTCAATCTCC GTGCCCGCCG CAGCCAGGAC TCAAGGTGTC
    CCGGCTGTGC TGAAAGTGAC TGGTCCTCAG GCTACCACGG GAACCCCGTT GGTCACCATG
    CGACCTGCCA GCCAGGCTGG GAAAGCCCCC GTGACTGTGA CCTCCCTTCC CGCAGGCGTG
    CGGATGGTTG TGCCTACACA GAGCGCCCAG GGGACAGTCA TTGGCAGCAG CCCGCAGATG
    AGTGGCATGG CTGCATTGGC AGCCGCGGCT GCTGCCACCC AGAAGATCCC TCCTTCCTCG
    GCACCCACGG TCCTGAGTGT CCCGGCTGGC ACCACTATCG TCAAAACCGT GGCCGTGACG
    CCGGGCACCA CCACGCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC
    AACCCAGCCA CTCGCATGCT GAAGACTGCA GCTGCCCAGG TGGGGACTTC CGTCTCTTCT
    GCCGCCAACA CATCTACCCG TCCCATCATC ACCGTACACA AGTCCGGAAC CGTGACAGTG
    GCCCAGCAAG CCCAGGTGGT GACCACAGTG GTGGGTGGGG TTACCAAGAC CATCACCCTG
    GTGAAGAGCC CCATCTCGGT CCCAGGAGGC AGTGCTCTGA TTTCCAATCT GGGCAAAGTA
    ATGTCAGTGG TCCAGACCAA ACCGGTTCAG ACTTCGGCAG TCACAGGCCA GGCATCTACA
    GGCCCGGTGA CTCAGATCAT CCAGACCAAA GGACCCTTGC CAGCCGGGAC CATCCTGAAG
    CTGGTGACGT CTGCAGATGG CAAGCCCACT ACCATCATCA CAACCACACA GGCCAGCGGG
    GCAGGGACTA AGCCTACCAT CCTGGGCATC AGCAGTGTGT CCCCCAGCAC CACCAAGCCC
    GGCACCACCA CTATCATTAA GACCATCCCC ATGTCGGCCA TCATCACACA GGCGGGCGCC
    ACGGGTAGGG GCCTTCCCCG GTGTGCTGGG GAGAAAGCAG GCGTGACTAG CAGTGCCGGC
    ATCAAGTCAC CCATCACCAT TATCACCACC AAGGTGATGA CTTCCGGAAC TGGAGCCCCC
    GCCAAAATCA TCACGGCTGT TCCCAAAATC GCTACGGGTC ACGGGCAGCA AGGAGTGACC
    CAGGTGGTGC TGAAGGGCGC ACCTGGCCAG CCAGGCACCA TCCTCCGCAC CGTGCCTATG
    GGGGGCGTCC GCCTGGTCAC CCCTGTTACC GTCTCTGCTG TCAAGCCTGC TGTCACCACA
    CTGGTTGTCA AGGGCACCAC AGGCGTCACG ACCCTGGGCA CGGTGACAGG CACCGTCTCC
    ACCAGCCTGG CCGGAGCCGG GGGCCATAGC ACCAGCGCCT CACTGGCCAC ACCCATCACC
    ACGTTGGGCA CCATCGCCAC CCTCTCAAGC CAGGTGATCA ACCCCACTGC CATCACTGTG
    TCTGCGGCGC AGACGACGCT GACAGCGGCC GCTGGGCTTA CCACACCCAC CATAACCATG
    CAGCCTGTCT CCCAGCCTAC CCAGGTGACT CTGATAACGG CCCCCAGCGG GGTGGAGGCC
    CAGCCTGTGC ACGACCTCCC AGTGTCCATT CTGGCCTCCC CTACTACAGA ACAGCCCACG
    GCCACGGTCA CCATCGCTGA TTCGGGCCAG GGTGATGTGC AGCCCGGCAC CGTGACCCTG
    GTGTGCTCCA ACCCGCCGTG CGAAACCCAC GAGACGGGCA CCACCAATAC AGCCACCACC
    ACCGTCGTCG CTAATCTGGG GGGGCACCCC CAGCCCACCC AAGTGCAGTT TGTCTGCGAC
    AGACAGGAGG CCACTGCTGC TCTCGTGACC TCCACGGTGG GCCAGCCGAA TGGCAGTGTA
    GTTCGTGTCT GCTCCAACCC GCCGTGTGAA ACCCACGAGA CCGGCACCAC CAGTACAGCT
    ACCACCGCCA TGTCTGGCAT CGGAGGCGGG CCGCGGCGAG ACATCCGGCT CGCCTGCGCG
    GCCACCACCA TTCCCACCGT GGTCCGGGTC AGCGTGACTG CCGGGGCGTC AGAGGGAGCT
    CAGGTTTCCA TCAAGCCCGC ATGCCAAACC CGTCAGACCA GTGCAACCAG CACCACCATG
    ACTGTGATGG CCACCGGGGC CCCGTGCTCG GCTGGCCCAC TCCTCAGACC AAGCGTGGCC
    CTCGAGGCCG CTGGCCGCGG TGCCACTCTC TTGCAGCTGG GGCCACTGAG TGCCCAAGTC
    AGGCCCGGTG GCGAGGAAAG GTCCCTTGCC GGCCTGGGCC CGCTGGTGTC TGTGGGGCGC
    CAGCTGGAAG TGCATCACAC GCACACGACC AACACGCCCA CTGTGGCCCG CTCCGCCATG
    GGTGCCGGGG AGCCCCACGA GCTGCTGGGG GCCCCCACAC TTGTGTACGA GAGCTCAGCC
    AGCGCCTCTG TGACTGCCGC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CGCCGTGACC
    CAGGTCTGCT CCAACCCCCC GTGTGAGACC CACGAGACGG GCACCACCCA CACACCCACC
    ACGGCCACAT CCGGAGGGGC TGCAGGCCAG CCAGAGGGCG GGCAGCAGCC TCCTGCCAGC
    CGGCCCTGTG AGACGCACCA GACCGCTTCC ACTGGCACGA CCATGTCCGT CAGCTTGGGC
    GCCCTGCTCC CGGATGCCGC CCCCTCCCAC AGGACCCTGG AGTCCAGCCT GGAGGTGGCA
    GTGCCACCCG CAGTCGCCCC TCAGGCCGGT GCTTCGTTGC TCACTCCTTT CCCGACGCAA
    AGGGTGTGCT CCAACCCACC CTGTGAGACG CATGAGACAG GCACTACGCA CACGGCCACC
    ACTGTCACCT CCAACATGAG TTCCAACCAA GACCCCCCAC CGCCTGCCGG CGACCAGGGA
    GAGGTGGAGA GCACCCAGGG CGACAGTGTG AATATCGCCA GTTCCAGTCC CATCACGACA
    ACAGTGTCCT CCACGCTGAC GCGGGCCGTG ACCACCGTGA CACAGTCCAC GCCAGTCCCA
    GGCCCTTCGG TGCCGAAGAT CTCATCTGTG ACTGAGACTG CCCCAGGGGC TCTGACCACC
    GAAGTCCCCA TCCCGGCCAC GATTACAGTG ACCATAGCCA ACACAGAAAC TTCTGACATG
    CCCTTCTCTG CTGTTGACAT CCTGCAGCCC CCAGAGGAGC TCCAGGCCTC TCCAGGGCCA
    CGCCAGCAGC TTCCACCACG GCAGCTCCTG CAGCCTGCCT CCGCGCCCTT GGTGGGGGAC
    TCCGCCGAGG TCCTGTCAGC CTCCCAGAGC CCTGAGCTCC AGGCCGCCGT GGATCTGAGC
    AGTACAGGGG ACCCGTCTTC AGGCCAGGAG CCTGCCAGCT CGGCTGTGGT GGCCACCGTG
    GTGGTCCAGC CACCGCCGCC CACCCAGTCC GAAGTAGACC AGTTGTCCCT CCCCCAAGAG
    CTGATGGCCG AAGCCCAGGC GGGCACCACC ACCCTCATGG TGACAGGGCT CACCCCAGAG
    GAGCTGGCAG TGACTGCTGC TGCTGAAGCG GCCGCGCAGG CTGCAGCCAC AGAGGAGGCC
    CAGGCCCTGG CCATACAGGC CGTGCTCCAG GCCGCACAGC AGGCTGTCAT GGCAGGCACC
    GGGGAGCCCA TGGATACTTC AGAGGCGGCC GCAGCGGTGA CACAGGCAGA GTTGAGCCAC
    CTGTCGGCCG AGGGCCAGGA AGGCCAGGCG ACCACTATCC CCATCGTGCT GACACAGCAG
    GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG CTCCAGGAAG CACAGGCCCA GCAGCAGCAG
    CACCACCTGC CCACGGAAGC ACTGGCCCCG GCTGACAGCC TCAATGACCC GACCATCGAG
    AGCAACTGCC TCAATGAGCT GGCCGGGGCT GTGCCCAGCA CCGTGAGCCT GCTGCCCCCC
    ACGGCCACTG AGAGCCTGGC CCCGTCCAAC ACATTTGTGG CCCCCCAGCC GGTCGTTGTG
    GCCAGCCCCG CGAAGCTGCA GGCCGCAGCC ACCCTGACGG AAGTGGCCAA TGGCATTGAG
    TCCCTGGGTG TGAAGCCAGA CCTGCCACCG CCACCTAGCA AAGCCCCTGT AAAGAAGGAG
    AACCAGTGGT TTGACGTGGG GGTCATTAAG GGTACCAATG TGATGGTGAC ACACTATTTC
    CTGCCACCCG AAGATGCTGT CCCGACTGAT GATGACTCGG GCACCGTGCC CGACTACAAC
    CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC ACTGCCTATA AGTTCCGCGT CGCCGGGATC
    AATGCCTGCG GCCGGGGGCC CTTCAGTGAG ATCTCAGCCT TTAAAACGTG TCTGCCTGGC
    TTCCCAGGGG CCCCGTGTGC CATTAAAATC AGCAAAAGTC CAGACGGTGC TCACCTCACC
    TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCAGTGTA CCTGGCCATC
    CAGAGCGCGC AGGCCGGTGG TGAGACCAAG AGCTCGACCC CGGCGCAGCT GGCCTTCATG
    CGGGTGTACT GCGGGCCCAG CCCCTCCTGC CTCGTGCAGT CCTCCAGCCT CTCCAACGCC
    CACATCGACT ACACCACCAA GCCCGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAGAAG
    GGCTACGGCC CAGCCACCCA AGTCAGGTGG TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC
    GCCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCCGC GCCAAAGAAA
    TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325776.1
    CDS78..5318
    Translation

    Target ORF information:

    RefSeq Version XM_015470290.1
    Organism Bos taurus(cattle)
    Definition Bos taurus host cell factor C1 (HCFC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTTGGG AGACCATCCT GATGGATACG CTGGAAGACA ATATTCCCCG GGCCCGTGCT 
    GGCCACTGTG CGGTAGCCAT CAATACCCGC CTTTACATTT GGAGTGGGCG TGACGGCTAC
    CGAAAGGCCT GGAATAACCA GGTCTGCTGC AAGGACCTCT GGTACCTGGA AACGGAGAAG
    CCGCCACCTC CAGCCCGGGT ACAGCTGGTG CGAGCCAACA CTAACTCCCT GGAGGTGAGC
    TGGGGGGCCG TGGCAACAGC CGACAGTTAC CTTCTGCAGC TCCAGAAATA TGACATTCCT
    GCCACGGCTG CCACTGCCAC CTCCCCCACA CCCAATCCAG TCCCGTCTGT GCCTGCCAAT
    CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCCG TGCAGCCGCT GACCCAAGTA
    GGCATCACGC TCCTGCCCCA GGCTGCTGCC GCGCCCCCGA CCACCACCAC CATCCAGGTC
    TTGCCGACGG TGCCTGGCAG CTCAATCTCC GTGCCCGCCG CAGCCAGGAC TCAAGGTGTC
    CCGGCTGTGC TGAAAGTGAC TGGTCCTCAG GCTACCACGG GAACCCCGTT GGTCACCATG
    CGACCTGCCA GCCAGGCTGG GAAAGCCCCC GTGACTGTGA CCTCCCTTCC CGCAGGCGTG
    CGGATGGTTG TGCCTACACA GAGCGCCCAG GGGACAGTCA TTGGCAGCAG CCCGCAGATG
    AGTGGCATGG CTGCATTGGC AGCCGCGGCT GCTGCCACCC AGAAGATCCC TCCTTCCTCG
    GCACCCACGG TCCTGAGTGT CCCGGCTGGC ACCACTATCG TCAAAACCGT GGCCGTGACG
    CCGGGCACCA CCACGCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC
    AACCCAGCCA CTCGCATGCT GAAGACTGCA GCTGCCCAGG TGGGGACTTC CGTCTCTTCT
    GCCGCCAACA CATCTACCCG TCCCATCATC ACCGTACACA AGTCCGGAAC CGTGACAGTG
    GCCCAGCAAG CCCAGGTGGT GACCACAGTG GTGGGTGGGG TTACCAAGAC CATCACCCTG
    GTGAAGAGCC CCATCTCGGT CCCAGGAGGC AGTGCTCTGA TTTCCAATCT GGGCAAAGTA
    ATGTCAGTGG TCCAGACCAA ACCGGTTCAG ACTTCGGCAG TCACAGGCCA GGCATCTACA
    GGCCCGGTGA CTCAGATCAT CCAGACCAAA GGACCCTTGC CAGCCGGGAC CATCCTGAAG
    CTGGTGACGT CTGCAGATGG CAAGCCCACT ACCATCATCA CAACCACACA GGCCAGCGGG
    GCAGGGACTA AGCCTACCAT CCTGGGCATC AGCAGTGTGT CCCCCAGCAC CACCAAGCCC
    GGCACCACCA CTATCATTAA GACCATCCCC ATGTCGGCCA TCATCACACA GGCGGGCGCC
    ACGGGTAGGG GCCTTCCCCG GTGTGCTGGG GAGAAAGCAG GCGTGACTAG CAGTGCCGGC
    ATCAAGTCAC CCATCACCAT TATCACCACC AAGGTGATGA CTTCCGGAAC TGGAGCCCCC
    GCCAAAATCA TCACGGCTGT TCCCAAAATC GCTACGGGTC ACGGGCAGCA AGGAGTGACC
    CAGGTGGTGC TGAAGGGCGC ACCTGGCCAG CCAGGCACCA TCCTCCGCAC CGTGCCTATG
    GGGGGCGTCC GCCTGGTCAC CCCTGTTACC GTCTCTGCTG TCAAGCCTGC TGTCACCACA
    CTGGTTGTCA AGGGCACCAC AGGCGTCACG ACCCTGGGCA CGGTGACAGG CACCGTCTCC
    ACCAGCCTGG CCGGAGCCGG GGGCCATAGC ACCAGCGCCT CACTGGCCAC ACCCATCACC
    ACGTTGGGCA CCATCGCCAC CCTCTCAAGC CAGGTGATCA ACCCCACTGC CATCACTGTG
    TCTGCGGCGC AGACGACGCT GACAGCGGCC GCTGGGCTTA CCACACCCAC CATAACCATG
    CAGCCTGTCT CCCAGCCTAC CCAGGTGACT CTGATAACGG CCCCCAGCGG GGTGGAGGCC
    CAGCCTGTGC ACGACCTCCC AGTGTCCATT CTGGCCTCCC CTACTACAGA ACAGCCCACG
    GCCACGGTCA CCATCGCTGA TTCGGGCCAG GGTGATGTGC AGCCCGGCAC CGTGACCCTG
    GTGTGCTCCA ACCCGCCGTG CGAAACCCAC GAGACGGGCA CCACCAATAC AGCCACCACC
    ACCGTCGTCG CTAATCTGGG GGGGCACCCC CAGCCCACCC AAGTGCAGTT TGTCTGCGAC
    AGACAGGAGG CCACTGCTGC TCTCGTGACC TCCACGGTGG GCCAGCCGAA TGGCAGTGTA
    GTTCGTGTCT GCTCCAACCC GCCGTGTGAA ACCCACGAGA CCGGCACCAC CAGTACAGCT
    ACCACCGCCA TGTCTGGCAT CGGAGGCGGG CCGCGGCGAG ACATCCGGCT CGCCTGCGCG
    GCCACCACCA TTCCCACCGT GGTCCGGGTC AGCGTGACTG CCGGGGCGTC AGAGGGAGCT
    CAGGTTTCCA TCAAGCCCGC ATGCCAAACC CGTCAGACCA GTGCAACCAG CACCACCATG
    ACTGTGATGG CCACCGGGGC CCCGTGCTCG GCTGGCCCAC TCCTCAGACC AAGCGTGGCC
    CTCGAGGCCG CTGGCCGCGG TGCCACTCTC TTGCAGCTGG GGCCACTGAG TGCCCAAGTC
    AGGCCCGGTG GCGAGGAAAG GTCCCTTGCC GGCCTGGGCC CGCTGGTGTC TGTGGGGCGC
    CAGCTGGAAG TGCATCACAC GCACACGACC AACACGCCCA CTGTGGCCCG CTCCGCCATG
    GGTGCCGGGG AGCCCCACGA GCTGCTGGGG GCCCCCACAC TTGTGTACGA GAGCTCAGCC
    AGCGCCTCTG TGACTGCCGC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CGCCGTGACC
    CAGGTCTGCT CCAACCCCCC GTGTGAGACC CACGAGACGG GCACCACCCA CACACCCACC
    ACGGCCACAT CCGGAGGGGC TGCAGGCCAG CCAGAGGGCG GGCAGCAGCC TCCTGCCAGC
    CGGCCCTGTG AGACGCACCA GACCGCTTCC ACTGGCACGA CCATGTCCGT CAGCTTGGGC
    GCCCTGCTCC CGGATGCCGC CCCCTCCCAC AGGACCCTGG AGTCCAGCCT GGAGGTGGCA
    GTGCCACCCG CAGTCGCCCC TCAGGCCGGT GCTTCGTTGC TCACTCCTTT CCCGACGCAA
    AGGGTGTGCT CCAACCCACC CTGTGAGACG CATGAGACAG GCACTACGCA CACGGCCACC
    ACTGTCACCT CCAACATGAG TTCCAACCAA GACCCCCCAC CGCCTGCCGG CGACCAGGGA
    GAGGTGGAGA GCACCCAGGG CGACAGTGTG AATATCGCCA GTTCCAGTCC CATCACGACA
    ACAGTGTCCT CCACGCTGAC GCGGGCCGTG ACCACCGTGA CACAGTCCAC GCCAGTCCCA
    GGCCCTTCGG TGCCGAAGAT CTCATCTGTG ACTGAGACTG CCCCAGGGGC TCTGACCACC
    GAAGTCCCCA TCCCGGCCAC GATTACAGTG ACCATAGCCA ACACAGAAAC TTCTGACATG
    CCCTTCTCTG CTGTTGACAT CCTGCAGCCC CCAGAGGAGC TCCAGGCCTC TCCAGGGCCA
    CGCCAGCAGC TTCCACCACG GCAGCTCCTG CAGCCTGCCT CCGCGCCCTT GGTGGGGGAC
    TCCGCCGAGG TCCTGTCAGC CTCCCAGAGC CCTGAGCTCC AGGCCGCCGT GGATCTGAGC
    AGTACAGGGG ACCCGTCTTC AGGCCAGGAG CCTGCCAGCT CGGCTGTGGT GGCCACCGTG
    GTGGTCCAGC CACCGCCGCC CACCCAGTCC GAAGTAGACC AGTTGTCCCT CCCCCAAGAG
    CTGATGGCCG AAGCCCAGGC GGGCACCACC ACCCTCATGG TGACAGGGCT CACCCCAGAG
    GAGCTGGCAG TGACTGCTGC TGCTGAAGCG GCCGCGCAGG CTGCAGCCAC AGAGGAGGCC
    CAGGCCCTGG CCATACAGGC CGTGCTCCAG GCCGCACAGC AGGCTGTCAT GGCAGGCACC
    GGGGAGCCCA TGGATACTTC AGAGGCGGCC GCAGCGGTGA CACAGGCAGA GTTGAGCCAC
    CTGTCGGCCG AGGGCCAGGA AGGCCAGGCG ACCACTATCC CCATCGTGCT GACACAGCAG
    GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG CTCCAGGAAG CACAGGCCCA GCAGCAGCAG
    CACCACCTGC CCACGGAAGC ACTGGCCCCG GCTGACAGCC TCAATGACCC GACCATCGAG
    AGCAACTGCC TCAATGAGCT GGCCGGGGCT GTGCCCAGCA CCGTGAGCCT GCTGCCCCCC
    ACGGCCACTG AGAGCCTGGC CCCGTCCAAC ACATTTGTGG CCCCCCAGCC GGTCGTTGTG
    GCCAGCCCCG CGAAGCTGCA GGCCGCAGCC ACCCTGACGG AAGTGGCCAA TGGCATTGAG
    TCCCTGGGTG TGAAGCCAGA CCTGCCACCG CCACCTAGCA AAGCCCCTGT AAAGAAGGAG
    AACCAGTGGT TTGACGTGGG GGTCATTAAG GGTACCAATG TGATGGTGAC ACACTATTTC
    CTGCCACCCG AAGATGCTGT CCCGACTGAT GATGACTCGG GCACCGTGCC CGACTACAAC
    CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC ACTGCCTATA AGTTCCGCGT CGCCGGGATC
    AATGCCTGCG GCCGGGGGCC CTTCAGTGAG ATCTCAGCCT TTAAAACGTG TCTGCCTGGC
    TTCCCAGGGG CCCCGTGTGC CATTAAAATC AGCAAAAGTC CAGACGGTGC TCACCTCACC
    TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCAGTGTA CCTGGCCATC
    CAGAGCGCGC AGGCCGGTGG TGAGACCAAG AGCTCGACCC CGGCGCAGCT GGCCTTCATG
    CGGGTGTACT GCGGGCCCAG CCCCTCCTGC CTCGTGCAGT CCTCCAGCCT CTCCAACGCC
    CACATCGACT ACACCACCAA GCCCGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAGAAG
    GGCTACGGCC CAGCCACCCA AGTCAGGTGG TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC
    GCCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCCGC GCCAAAGAAA
    TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.