Hcfc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcfc1
Entrez Gene ID	15161
Full Name	host cell factor C1
Synonyms	AW060991,HCF,HCF1
General protein information	Preferred Names host cell factor C1 Names host cell factor 1 VP16 accessory protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A7.3\|X 37.52 cM
Summary	This gene encodes a transcription cofactor that regulates the progression of cell cycle and maintain the ability of embryonic stem cells to self-renew. The encoded protein is a large precursor that undergoes site-specific proteolytic cleavage to yield N- and C-terminal chains that form a non-covalent heterodimer. The encoded protein has been implicated in the regulation of expression of immediate early genes after herpes simplex virus infection and glucose-stimulated secretion of insulin by pancreatic beta cells. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527835.1	XP_006527898.1	host cell factor 1 isoform X1
XM_006527836.1	XP_006527899.1	host cell factor 1 isoform X2
XM_006527835.2	XP_006527898.1	host cell factor 1 isoform X1
XM_017318387.2	XP_017173876.1	host cell factor 1 isoform X3
NM_008224.4	NP_032250.2	host cell factor 1 precursor
XM_017318387.1	XP_017173876.1	host cell factor 1 isoform X3
XM_006527836.2	XP_006527899.1	host cell factor 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HCFC1	XP_521330.3
Rattus norvegicus (Norway rat)	Hcfc1	NP_001132979.1
Mus musculus (house mouse)	Hcfc1	NP_032250.2
Danio rerio (zebrafish)	hcfc1b	NP_001122009.1
Bos taurus (cattle)	HCFC1	XP_002707954.1
Homo sapiens (human)	HCFC1	NP_005325.2
Canis lupus familiaris (dog)	HCFC1	XP_005642062.1
Caenorhabditis elegans (roundworm)	hcf-1	NP_501279.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification.
Minocha S, Bessonnard S, Sung TL, Moret C, Constam DB, Herr W
Developmental biology418(1)75-88(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

male epiblast-specific loss of Hcfc1 leads to a developmental arrest at E6.5 with a rapid progressive cell-cycle exit and an associated failure of anterior visceral endoderm migration and primitive streak formation.
Title: Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification.

Beyond its well-known role in adding beta-O-GlcNAc to serine and threonine residues of nuclear and cytoplasmic proteins, OGT also acts as a protease in the maturation of the cell cycle regulator, HCF-1, and serves as an integral member of several protein complexes, many of them linked to gene expression. (Review)
Title: The Biochemistry of O-GlcNAc Transferase: Which Functions Make It Essential in Mammalian Cells?

survival of the heterozygous Hcfc1(epiKO/+) female embryos llustrates the developmental plasticity of the post-implantation mouse embryo - in this instance, permitting survival of females heterozygous for an X-linked embryonic lethal allele
Title: Compensatory embryonic response to allele-specific inactivation of the murine X-linked gene Hcfc1.

our work identifies plausible cellular consequences of missense HCFC1 variants and identifies likely and relevant disease mechanisms that converge on embryonic stages of brain development.
Title: HCFC1 loss-of-function mutations disrupt neuronal and neural progenitor cells of the developing brain.

These results indicate that HCF-1 represents a novel transcriptional regulator required for maintaining pancreatic b-cell function.
Title: The transcriptional co-regulator HCF-1 is required for INS-1 ?-cell glucose-stimulated insulin secretion.

The following Hcfc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcfc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu56157	XM_006527835.2 Latest version!	Mus musculus host cell factor C1 (Hcfc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu56158	XM_006527836.2 Latest version!	Mus musculus host cell factor C1 (Hcfc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu83077	XM_017318387.2 Latest version!	Mus musculus host cell factor C1 (Hcfc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu16665	NM_008224.4 Latest version!	Mus musculus host cell factor C1 (Hcfc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1504.30 $2149.00
View Old Accession Versions >>
OMu56157	XM_006527835.1	Mus musculus host cell factor C1 (Hcfc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu83077	XM_017318387.1	Mus musculus host cell factor C1 (Hcfc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu56158	XM_006527836.1	Mus musculus host cell factor C1 (Hcfc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcfc1 ( XM_006527835.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527898.1 Mus musculus host cell factor C1 (Hcfc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56157
Clone ID Related Accession (Same CDS sequence)	XM_006527835.1 , XM_006527835.2
Accession Version	XM_006527835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCCAT GGATACATCT GAAGCAGCAG CAGCAGTGAC ACAAGCAGAA 
CTGGGTCACC TTTCAGCTGA AGGCCAAGAG GGTCAGGCTA CCACCATACC CATTGTGTTG 
ACACAGCAGG AGCTTGCAGC CCTGGTGCAG CAGCAGCAGC AGCTCCAGGA GGCTCAAGCT 
CAAGCCCAGC AACAGCACCA TCTTCCCACT GAGGCTCTGG CCCCAGCTGA CAGTCTCAAT 
GACCCATCCA TCGAGAGCAA CTGCCTCAAC GAGTTAGCTA GTGCTGTCCC AAGCACCGTG 
GCTTTGCTAC CCTCAACAGC TACCGAGAGC CTGGCTCCAT CTAACACATT TGTGGCTCCC 
CAGCCTGTTG TAGCTAGTCC AGCAAAGATG CAGGCTGCAG CTACCCTTAC TGAAGTGGCC 
AATGGCATTG AGTCCCTGGG TGTGAAACCG GACTTGCCAC CCCCACCCAG CAAAGCCCCT 
GTGAAAAAGG AGAACCAGTG GTTTGATGTG GGGGTCATTA AGGGTACCAG TGTAATGGTG 
ACACACTATT TTCTGCCACC AGATGATGCT GTTCAGTCAG ATGATGACTC AGGCACGGTC 
CCAGACTATA ACCAGCTAAA GAAGCAGGAG CTACAGCCAG GCACGGCTTA CAAATTTCGA 
GTTGCTGGAA TCAATGCTTG TGGCCGGGGA CCCTTCAGTG AGATCTCAGC CTTTAAGACT 
TGTCTGCCTG GGTTCCCAGG GGCTCCTTGT GCTATTAAAA TCAGCAAGAG CCCAGATGGT 
GCTCACCTCA CCTGGGAGCC ACCGTCTGTG ACCTCCGGCA AGATCATCGA GTACTCTGTG 
TACCTGGCCA TCCAGAGCTC ACAGGCCAGT GGTGAGCCAA AGAGCTCCAC CCCAGCCCAG 
CTGGCCTTCA TGCGAGTGTA CTGTGGGCCT AGCCCTTCCT GCCTAGTGCA GTCCTCCAGC 
CTCTCCAACG CCCACATTGA CTATACTACA AAGCCTGCCA TCATCTTCCG CATTGCTGCC 
CGCAATGAAA AGGGCTACGG CCCTGCCACA CAAGTGAGGT GGTTGCAAGA AACTAGTAAA 
GACAGCTCTG GCACCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCT 
GCTCCAAAGA AGTCTAAGGC TGATGGTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527898.1
CDS	993..7265
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPNISSLTETTPGALNSEVPIPATITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006527835.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527835.2

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCCAT GGATACATCT GAAGCAGCAG CAGCAGTGAC ACAAGCAGAA 
CTGGGTCACC TTTCAGCTGA AGGCCAAGAG GGTCAGGCTA CCACCATACC CATTGTGTTG 
ACACAGCAGG AGCTTGCAGC CCTGGTGCAG CAGCAGCAGC AGCTCCAGGA GGCTCAAGCT 
CAAGCCCAGC AACAGCACCA TCTTCCCACT GAGGCTCTGG CCCCAGCTGA CAGTCTCAAT 
GACCCATCCA TCGAGAGCAA CTGCCTCAAC GAGTTAGCTA GTGCTGTCCC AAGCACCGTG 
GCTTTGCTAC CCTCAACAGC TACCGAGAGC CTGGCTCCAT CTAACACATT TGTGGCTCCC 
CAGCCTGTTG TAGCTAGTCC AGCAAAGATG CAGGCTGCAG CTACCCTTAC TGAAGTGGCC 
AATGGCATTG AGTCCCTGGG TGTGAAACCG GACTTGCCAC CCCCACCCAG CAAAGCCCCT 
GTGAAAAAGG AGAACCAGTG GTTTGATGTG GGGGTCATTA AGGGTACCAG TGTAATGGTG 
ACACACTATT TTCTGCCACC AGATGATGCT GTTCAGTCAG ATGATGACTC AGGCACGGTC 
CCAGACTATA ACCAGCTAAA GAAGCAGGAG CTACAGCCAG GCACGGCTTA CAAATTTCGA 
GTTGCTGGAA TCAATGCTTG TGGCCGGGGA CCCTTCAGTG AGATCTCAGC CTTTAAGACT 
TGTCTGCCTG GGTTCCCAGG GGCTCCTTGT GCTATTAAAA TCAGCAAGAG CCCAGATGGT 
GCTCACCTCA CCTGGGAGCC ACCGTCTGTG ACCTCCGGCA AGATCATCGA GTACTCTGTG 
TACCTGGCCA TCCAGAGCTC ACAGGCCAGT GGTGAGCCAA AGAGCTCCAC CCCAGCCCAG 
CTGGCCTTCA TGCGAGTGTA CTGTGGGCCT AGCCCTTCCT GCCTAGTGCA GTCCTCCAGC 
CTCTCCAACG CCCACATTGA CTATACTACA AAGCCTGCCA TCATCTTCCG CATTGCTGCC 
CGCAATGAAA AGGGCTACGG CCCTGCCACA CAAGTGAGGT GGTTGCAAGA AACTAGTAAA 
GACAGCTCTG GCACCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCT 
GCTCCAAAGA AGTCTAAGGC TGATGGTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcfc1 ( XM_006527836.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527899.1 Mus musculus host cell factor C1 (Hcfc1), transcript variant X2, mRNA.

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CloneID	OMu56158
Clone ID Related Accession (Same CDS sequence)	XM_006527836.1 , XM_006527836.2
Accession Version	XM_006527836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCCATGGA TACATCTGAA GCAGCAGCAG CAGTGACACA AGCAGAACTG 
GGTCACCTTT CAGCTGAAGG CCAAGAGGGT CAGGCTACCA CCATACCCAT TGTGTTGACA 
CAGCAGGAGC TTGCAGCCCT GGTGCAGCAG CAGCAGCAGC TCCAGGAGGC TCAAGCTCAA 
GCCCAGCAAC AGCACCATCT TCCCACTGAG GCTCTGGCCC CAGCTGACAG TCTCAATGAC 
CCATCCATCG AGAGCAACTG CCTCAACGAG TTAGCTAGTG CTGTCCCAAG CACCGTGGCT 
TTGCTACCCT CAACAGCTAC CGAGAGCCTG GCTCCATCTA ACACATTTGT GGCTCCCCAG 
CCTGTTGTAG CTAGTCCAGC AAAGATGCAG GCTGCAGCTA CCCTTACTGA AGTGGCCAAT 
GGCATTGAGT CCCTGGGTGT GAAACCGGAC TTGCCACCCC CACCCAGCAA AGCCCCTGTG 
AAAAAGGAGA ACCAGTGGTT TGATGTGGGG GTCATTAAGG GTACCAGTGT AATGGTGACA 
CACTATTTTC TGCCACCAGA TGATGCTGTT CAGTCAGATG ATGACTCAGG CACGGTCCCA 
GACTATAACC AGCTAAAGAA GCAGGAGCTA CAGCCAGGCA CGGCTTACAA ATTTCGAGTT 
GCTGGAATCA ATGCTTGTGG CCGGGGACCC TTCAGTGAGA TCTCAGCCTT TAAGACTTGT 
CTGCCTGGGT TCCCAGGGGC TCCTTGTGCT ATTAAAATCA GCAAGAGCCC AGATGGTGCT 
CACCTCACCT GGGAGCCACC GTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCTGTGTAC 
CTGGCCATCC AGAGCTCACA GGCCAGTGGT GAGCCAAAGA GCTCCACCCC AGCCCAGCTG 
GCCTTCATGC GAGTGTACTG TGGGCCTAGC CCTTCCTGCC TAGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATTGACTA TACTACAAAG CCTGCCATCA TCTTCCGCAT TGCTGCCCGC 
AATGAAAAGG GCTACGGCCC TGCCACACAA GTGAGGTGGT TGCAAGAAAC TAGTAAAGAC 
AGCTCTGGCA CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCTGCT 
CCAAAGAAGT CTAAGGCTGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527899.1
CDS	993..7262
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPNISSLTETTPGALNSEVPIPATITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006527836.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527836.2

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCCATGGA TACATCTGAA GCAGCAGCAG CAGTGACACA AGCAGAACTG 
GGTCACCTTT CAGCTGAAGG CCAAGAGGGT CAGGCTACCA CCATACCCAT TGTGTTGACA 
CAGCAGGAGC TTGCAGCCCT GGTGCAGCAG CAGCAGCAGC TCCAGGAGGC TCAAGCTCAA 
GCCCAGCAAC AGCACCATCT TCCCACTGAG GCTCTGGCCC CAGCTGACAG TCTCAATGAC 
CCATCCATCG AGAGCAACTG CCTCAACGAG TTAGCTAGTG CTGTCCCAAG CACCGTGGCT 
TTGCTACCCT CAACAGCTAC CGAGAGCCTG GCTCCATCTA ACACATTTGT GGCTCCCCAG 
CCTGTTGTAG CTAGTCCAGC AAAGATGCAG GCTGCAGCTA CCCTTACTGA AGTGGCCAAT 
GGCATTGAGT CCCTGGGTGT GAAACCGGAC TTGCCACCCC CACCCAGCAA AGCCCCTGTG 
AAAAAGGAGA ACCAGTGGTT TGATGTGGGG GTCATTAAGG GTACCAGTGT AATGGTGACA 
CACTATTTTC TGCCACCAGA TGATGCTGTT CAGTCAGATG ATGACTCAGG CACGGTCCCA 
GACTATAACC AGCTAAAGAA GCAGGAGCTA CAGCCAGGCA CGGCTTACAA ATTTCGAGTT 
GCTGGAATCA ATGCTTGTGG CCGGGGACCC TTCAGTGAGA TCTCAGCCTT TAAGACTTGT 
CTGCCTGGGT TCCCAGGGGC TCCTTGTGCT ATTAAAATCA GCAAGAGCCC AGATGGTGCT 
CACCTCACCT GGGAGCCACC GTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCTGTGTAC 
CTGGCCATCC AGAGCTCACA GGCCAGTGGT GAGCCAAAGA GCTCCACCCC AGCCCAGCTG 
GCCTTCATGC GAGTGTACTG TGGGCCTAGC CCTTCCTGCC TAGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATTGACTA TACTACAAAG CCTGCCATCA TCTTCCGCAT TGCTGCCCGC 
AATGAAAAGG GCTACGGCCC TGCCACACAA GTGAGGTGGT TGCAAGAAAC TAGTAAAGAC 
AGCTCTGGCA CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCTGCT 
CCAAAGAAGT CTAAGGCTGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcfc1 ( XM_017318387.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173876.1 Mus musculus host cell factor C1 (Hcfc1), transcript variant X3, mRNA.

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CloneID	OMu83077
Clone ID Related Accession (Same CDS sequence)	XM_017318387.2 , XM_017318387.1
Accession Version	XM_017318387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGCCCCCA GAGGAACTCC AGGTCTCACC AGGGCCTCGC 
CAGCAGCTGC CTCCACGGCA ACTCCTGCAG TCTGCCTCCA CACCCCTGAT GGGGGAGTCT 
ACTGAGGTCC TGTCAGCCTC CCAGACCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGC 
ACTGGGGACC CATCTTCAGG CCAGGAGCCT ACCACCTCTG CTGTCGTGGC CACTGTGGTG 
GTCCAACCAC CCCCACCCAC ACAGTCTGAA GTAGACCAGT TATCACTTCC CCAAGAGCTG 
ATGGCTGAAG CCCAGGCGGG CACCACAACC CTTATGGTAA CAGGGCTCAC TCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCT GCTCAAGCTG CAGCCACTGA AGAAGCCCAA 
GCCTTGGCCA TCCAGGCTGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCCATGG ATACATCTGA AGCAGCAGCA GCAGTGACAC AAGCAGAACT GGGTCACCTT 
TCAGCTGAAG GCCAAGAGGG TCAGGCTACC ACCATACCCA TTGTGTTGAC ACAGCAGGAG 
CTTGCAGCCC TGGTGCAGCA GCAGCAGCAG CTCCAGGAGG CTCAAGCTCA AGCCCAGCAA 
CAGCACCATC TTCCCACTGA GGCTCTGGCC CCAGCTGACA GTCTCAATGA CCCATCCATC 
GAGAGCAACT GCCTCAACGA GTTAGCTAGT GCTGTCCCAA GCACCGTGGC TTTGCTACCC 
TCAACAGCTA CCGAGAGCCT GGCTCCATCT AACACATTTG TGGCTCCCCA GCCTGTTGTA 
GCTAGTCCAG CAAAGATGCA GGCTGCAGCT ACCCTTACTG AAGTGGCCAA TGGCATTGAG 
TCCCTGGGTG TGAAACCGGA CTTGCCACCC CCACCCAGCA AAGCCCCTGT GAAAAAGGAG 
AACCAGTGGT TTGATGTGGG GGTCATTAAG GGTACCAGTG TAATGGTGAC ACACTATTTT 
CTGCCACCAG ATGATGCTGT TCAGTCAGAT GATGACTCAG GCACGGTCCC AGACTATAAC 
CAGCTAAAGA AGCAGGAGCT ACAGCCAGGC ACGGCTTACA AATTTCGAGT TGCTGGAATC 
AATGCTTGTG GCCGGGGACC CTTCAGTGAG ATCTCAGCCT TTAAGACTTG TCTGCCTGGG 
TTCCCAGGGG CTCCTTGTGC TATTAAAATC AGCAAGAGCC CAGATGGTGC TCACCTCACC 
TGGGAGCCAC CGTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCTGTGTA CCTGGCCATC 
CAGAGCTCAC AGGCCAGTGG TGAGCCAAAG AGCTCCACCC CAGCCCAGCT GGCCTTCATG 
CGAGTGTACT GTGGGCCTAG CCCTTCCTGC CTAGTGCAGT CCTCCAGCCT CTCCAACGCC 
CACATTGACT ATACTACAAA GCCTGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAAAAG 
GGCTACGGCC CTGCCACACA AGTGAGGTGG TTGCAAGAAA CTAGTAAAGA CAGCTCTGGC 
ACCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCTGC TCCAAAGAAG 
TCTAAGGCTG ATGGTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173876.1
CDS	993..7133
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_017318387.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318387.2

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGCCCCCA GAGGAACTCC AGGTCTCACC AGGGCCTCGC 
CAGCAGCTGC CTCCACGGCA ACTCCTGCAG TCTGCCTCCA CACCCCTGAT GGGGGAGTCT 
ACTGAGGTCC TGTCAGCCTC CCAGACCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGC 
ACTGGGGACC CATCTTCAGG CCAGGAGCCT ACCACCTCTG CTGTCGTGGC CACTGTGGTG 
GTCCAACCAC CCCCACCCAC ACAGTCTGAA GTAGACCAGT TATCACTTCC CCAAGAGCTG 
ATGGCTGAAG CCCAGGCGGG CACCACAACC CTTATGGTAA CAGGGCTCAC TCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCT GCTCAAGCTG CAGCCACTGA AGAAGCCCAA 
GCCTTGGCCA TCCAGGCTGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCCATGG ATACATCTGA AGCAGCAGCA GCAGTGACAC AAGCAGAACT GGGTCACCTT 
TCAGCTGAAG GCCAAGAGGG TCAGGCTACC ACCATACCCA TTGTGTTGAC ACAGCAGGAG 
CTTGCAGCCC TGGTGCAGCA GCAGCAGCAG CTCCAGGAGG CTCAAGCTCA AGCCCAGCAA 
CAGCACCATC TTCCCACTGA GGCTCTGGCC CCAGCTGACA GTCTCAATGA CCCATCCATC 
GAGAGCAACT GCCTCAACGA GTTAGCTAGT GCTGTCCCAA GCACCGTGGC TTTGCTACCC 
TCAACAGCTA CCGAGAGCCT GGCTCCATCT AACACATTTG TGGCTCCCCA GCCTGTTGTA 
GCTAGTCCAG CAAAGATGCA GGCTGCAGCT ACCCTTACTG AAGTGGCCAA TGGCATTGAG 
TCCCTGGGTG TGAAACCGGA CTTGCCACCC CCACCCAGCA AAGCCCCTGT GAAAAAGGAG 
AACCAGTGGT TTGATGTGGG GGTCATTAAG GGTACCAGTG TAATGGTGAC ACACTATTTT 
CTGCCACCAG ATGATGCTGT TCAGTCAGAT GATGACTCAG GCACGGTCCC AGACTATAAC 
CAGCTAAAGA AGCAGGAGCT ACAGCCAGGC ACGGCTTACA AATTTCGAGT TGCTGGAATC 
AATGCTTGTG GCCGGGGACC CTTCAGTGAG ATCTCAGCCT TTAAGACTTG TCTGCCTGGG 
TTCCCAGGGG CTCCTTGTGC TATTAAAATC AGCAAGAGCC CAGATGGTGC TCACCTCACC 
TGGGAGCCAC CGTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCTGTGTA CCTGGCCATC 
CAGAGCTCAC AGGCCAGTGG TGAGCCAAAG AGCTCCACCC CAGCCCAGCT GGCCTTCATG 
CGAGTGTACT GTGGGCCTAG CCCTTCCTGC CTAGTGCAGT CCTCCAGCCT CTCCAACGCC 
CACATTGACT ATACTACAAA GCCTGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAAAAG 
GGCTACGGCC CTGCCACACA AGTGAGGTGG TTGCAAGAAA CTAGTAAAGA CAGCTCTGGC 
ACCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCTGC TCCAAAGAAG 
TCTAAGGCTG ATGGTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcfc1 ( NM_008224.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032250.2 Mus musculus host cell factor C1 (Hcfc1), mRNA.

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CloneID	OMu16665
Clone ID Related Accession (Same CDS sequence)	NM_008224.4
Accession Version	NM_008224.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	host cell factor 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL672002.14. On Mar 13, 2014 this sequence version replaced NM_008224.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGCCCCCA GAGGAACTCC AGGTCTCACC AGGGCCTCGC 
CAGCAGCTGC CTCCACGGCA ACTCCTGCAG TCTGCCTCCA CACCCCTGAT GGGGGAGTCT 
ACTGAGGTCC TGTCAGCCTC CCAGACCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGC 
ACTGGGGACC CATCTTCAGG CCAGGAGCCT ACCACCTCTG CTGTCGTGGC CACTGTGGTG 
GTCCAACCAC CCCCACCCAC ACAGTCTGAA GTAGACCAGT TATCACTTCC CCAAGAGCTG 
ATGGCTGAAG CCCAGGCGGG CACCACAACC CTTATGGTAA CAGGGCTCAC TCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCT GCTCAAGCTG CAGCCACTGA AGAAGCCCAA 
GCCTTGGCCA TCCAGGCTGT GCTCCAGGCC GCACAGCAGG CTGTCATGGG CACTGGGGAG 
CCCATGGATA CATCTGAAGC AGCAGCAGCA GTGACACAAG CAGAACTGGG TCACCTTTCA 
GCTGAAGGCC AAGAGGGTCA GGCTACCACC ATACCCATTG TGTTGACACA GCAGGAGCTT 
GCAGCCCTGG TGCAGCAGCA GCAGCAGCTC CAGGAGGCTC AAGCTCAAGC CCAGCAACAG 
CACCATCTTC CCACTGAGGC TCTGGCCCCA GCTGACAGTC TCAATGACCC ATCCATCGAG 
AGCAACTGCC TCAACGAGTT AGCTAGTGCT GTCCCAAGCA CCGTGGCTTT GCTACCCTCA 
ACAGCTACCG AGAGCCTGGC TCCATCTAAC ACATTTGTGG CTCCCCAGCC TGTTGTAGCT 
AGTCCAGCAA AGATGCAGGC TGCAGCTACC CTTACTGAAG TGGCCAATGG CATTGAGTCC 
CTGGGTGTGA AACCGGACTT GCCACCCCCA CCCAGCAAAG CCCCTGTGAA AAAGGAGAAC 
CAGTGGTTTG ATGTGGGGGT CATTAAGGGT ACCAGTGTAA TGGTGACACA CTATTTTCTG 
CCACCAGATG ATGCTGTTCA GTCAGATGAT GACTCAGGCA CGGTCCCAGA CTATAACCAG 
CTAAAGAAGC AGGAGCTACA GCCAGGCACG GCTTACAAAT TTCGAGTTGC TGGAATCAAT 
GCTTGTGGCC GGGGACCCTT CAGTGAGATC TCAGCCTTTA AGACTTGTCT GCCTGGGTTC 
CCAGGGGCTC CTTGTGCTAT TAAAATCAGC AAGAGCCCAG ATGGTGCTCA CCTCACCTGG 
GAGCCACCGT CTGTGACCTC CGGCAAGATC ATCGAGTACT CTGTGTACCT GGCCATCCAG 
AGCTCACAGG CCAGTGGTGA GCCAAAGAGC TCCACCCCAG CCCAGCTGGC CTTCATGCGA 
GTGTACTGTG GGCCTAGCCC TTCCTGCCTA GTGCAGTCCT CCAGCCTCTC CAACGCCCAC 
ATTGACTATA CTACAAAGCC TGCCATCATC TTCCGCATTG CTGCCCGCAA TGAAAAGGGC 
TACGGCCCTG CCACACAAGT GAGGTGGTTG CAAGAAACTA GTAAAGACAG CTCTGGCACC 
AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCTGCTCC AAAGAAGTCT 
AAGGCTGATG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032250.2
CDS	355..6492
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	NM_008224.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008224.4

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGCCCCCA GAGGAACTCC AGGTCTCACC AGGGCCTCGC 
CAGCAGCTGC CTCCACGGCA ACTCCTGCAG TCTGCCTCCA CACCCCTGAT GGGGGAGTCT 
ACTGAGGTCC TGTCAGCCTC CCAGACCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGC 
ACTGGGGACC CATCTTCAGG CCAGGAGCCT ACCACCTCTG CTGTCGTGGC CACTGTGGTG 
GTCCAACCAC CCCCACCCAC ACAGTCTGAA GTAGACCAGT TATCACTTCC CCAAGAGCTG 
ATGGCTGAAG CCCAGGCGGG CACCACAACC CTTATGGTAA CAGGGCTCAC TCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCT GCTCAAGCTG CAGCCACTGA AGAAGCCCAA 
GCCTTGGCCA TCCAGGCTGT GCTCCAGGCC GCACAGCAGG CTGTCATGGG CACTGGGGAG 
CCCATGGATA CATCTGAAGC AGCAGCAGCA GTGACACAAG CAGAACTGGG TCACCTTTCA 
GCTGAAGGCC AAGAGGGTCA GGCTACCACC ATACCCATTG TGTTGACACA GCAGGAGCTT 
GCAGCCCTGG TGCAGCAGCA GCAGCAGCTC CAGGAGGCTC AAGCTCAAGC CCAGCAACAG 
CACCATCTTC CCACTGAGGC TCTGGCCCCA GCTGACAGTC TCAATGACCC ATCCATCGAG 
AGCAACTGCC TCAACGAGTT AGCTAGTGCT GTCCCAAGCA CCGTGGCTTT GCTACCCTCA 
ACAGCTACCG AGAGCCTGGC TCCATCTAAC ACATTTGTGG CTCCCCAGCC TGTTGTAGCT 
AGTCCAGCAA AGATGCAGGC TGCAGCTACC CTTACTGAAG TGGCCAATGG CATTGAGTCC 
CTGGGTGTGA AACCGGACTT GCCACCCCCA CCCAGCAAAG CCCCTGTGAA AAAGGAGAAC 
CAGTGGTTTG ATGTGGGGGT CATTAAGGGT ACCAGTGTAA TGGTGACACA CTATTTTCTG 
CCACCAGATG ATGCTGTTCA GTCAGATGAT GACTCAGGCA CGGTCCCAGA CTATAACCAG 
CTAAAGAAGC AGGAGCTACA GCCAGGCACG GCTTACAAAT TTCGAGTTGC TGGAATCAAT 
GCTTGTGGCC GGGGACCCTT CAGTGAGATC TCAGCCTTTA AGACTTGTCT GCCTGGGTTC 
CCAGGGGCTC CTTGTGCTAT TAAAATCAGC AAGAGCCCAG ATGGTGCTCA CCTCACCTGG 
GAGCCACCGT CTGTGACCTC CGGCAAGATC ATCGAGTACT CTGTGTACCT GGCCATCCAG 
AGCTCACAGG CCAGTGGTGA GCCAAAGAGC TCCACCCCAG CCCAGCTGGC CTTCATGCGA 
GTGTACTGTG GGCCTAGCCC TTCCTGCCTA GTGCAGTCCT CCAGCCTCTC CAACGCCCAC 
ATTGACTATA CTACAAAGCC TGCCATCATC TTCCGCATTG CTGCCCGCAA TGAAAAGGGC 
TACGGCCCTG CCACACAAGT GAGGTGGTTG CAAGAAACTA GTAAAGACAG CTCTGGCACC 
AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCTGCTCC AAAGAAGTCT 
AAGGCTGATG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcfc1 ( XM_006527835.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527898.1 Mus musculus host cell factor C1 (Hcfc1), transcript variant X1, mRNA.

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CloneID	OMu56157
Clone ID Related Accession (Same CDS sequence)	XM_006527835.1 , XM_006527835.2
Accession Version	XM_006527835.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCCAT GGATACATCT GAAGCAGCAG CAGCAGTGAC ACAAGCAGAA 
CTGGGTCACC TTTCAGCTGA AGGCCAAGAG GGTCAGGCTA CCACCATACC CATTGTGTTG 
ACACAGCAGG AGCTTGCAGC CCTGGTGCAG CAGCAGCAGC AGCTCCAGGA GGCTCAAGCT 
CAAGCCCAGC AACAGCACCA TCTTCCCACT GAGGCTCTGG CCCCAGCTGA CAGTCTCAAT 
GACCCATCCA TCGAGAGCAA CTGCCTCAAC GAGTTAGCTA GTGCTGTCCC AAGCACCGTG 
GCTTTGCTAC CCTCAACAGC TACCGAGAGC CTGGCTCCAT CTAACACATT TGTGGCTCCC 
CAGCCTGTTG TAGCTAGTCC AGCAAAGATG CAGGCTGCAG CTACCCTTAC TGAAGTGGCC 
AATGGCATTG AGTCCCTGGG TGTGAAACCG GACTTGCCAC CCCCACCCAG CAAAGCCCCT 
GTGAAAAAGG AGAACCAGTG GTTTGATGTG GGGGTCATTA AGGGTACCAG TGTAATGGTG 
ACACACTATT TTCTGCCACC AGATGATGCT GTTCAGTCAG ATGATGACTC AGGCACGGTC 
CCAGACTATA ACCAGCTAAA GAAGCAGGAG CTACAGCCAG GCACGGCTTA CAAATTTCGA 
GTTGCTGGAA TCAATGCTTG TGGCCGGGGA CCCTTCAGTG AGATCTCAGC CTTTAAGACT 
TGTCTGCCTG GGTTCCCAGG GGCTCCTTGT GCTATTAAAA TCAGCAAGAG CCCAGATGGT 
GCTCACCTCA CCTGGGAGCC ACCGTCTGTG ACCTCCGGCA AGATCATCGA GTACTCTGTG 
TACCTGGCCA TCCAGAGCTC ACAGGCCAGT GGTGAGCCAA AGAGCTCCAC CCCAGCCCAG 
CTGGCCTTCA TGCGAGTGTA CTGTGGGCCT AGCCCTTCCT GCCTAGTGCA GTCCTCCAGC 
CTCTCCAACG CCCACATTGA CTATACTACA AAGCCTGCCA TCATCTTCCG CATTGCTGCC 
CGCAATGAAA AGGGCTACGG CCCTGCCACA CAAGTGAGGT GGTTGCAAGA AACTAGTAAA 
GACAGCTCTG GCACCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCT 
GCTCCAAAGA AGTCTAAGGC TGATGGTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527898.1
CDS	1133..7405
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPNISSLTETTPGALNSEVPIPATITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006527835.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527835.1

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GCAGGCACTG GGGAGCCCAT GGATACATCT GAAGCAGCAG CAGCAGTGAC ACAAGCAGAA 
CTGGGTCACC TTTCAGCTGA AGGCCAAGAG GGTCAGGCTA CCACCATACC CATTGTGTTG 
ACACAGCAGG AGCTTGCAGC CCTGGTGCAG CAGCAGCAGC AGCTCCAGGA GGCTCAAGCT 
CAAGCCCAGC AACAGCACCA TCTTCCCACT GAGGCTCTGG CCCCAGCTGA CAGTCTCAAT 
GACCCATCCA TCGAGAGCAA CTGCCTCAAC GAGTTAGCTA GTGCTGTCCC AAGCACCGTG 
GCTTTGCTAC CCTCAACAGC TACCGAGAGC CTGGCTCCAT CTAACACATT TGTGGCTCCC 
CAGCCTGTTG TAGCTAGTCC AGCAAAGATG CAGGCTGCAG CTACCCTTAC TGAAGTGGCC 
AATGGCATTG AGTCCCTGGG TGTGAAACCG GACTTGCCAC CCCCACCCAG CAAAGCCCCT 
GTGAAAAAGG AGAACCAGTG GTTTGATGTG GGGGTCATTA AGGGTACCAG TGTAATGGTG 
ACACACTATT TTCTGCCACC AGATGATGCT GTTCAGTCAG ATGATGACTC AGGCACGGTC 
CCAGACTATA ACCAGCTAAA GAAGCAGGAG CTACAGCCAG GCACGGCTTA CAAATTTCGA 
GTTGCTGGAA TCAATGCTTG TGGCCGGGGA CCCTTCAGTG AGATCTCAGC CTTTAAGACT 
TGTCTGCCTG GGTTCCCAGG GGCTCCTTGT GCTATTAAAA TCAGCAAGAG CCCAGATGGT 
GCTCACCTCA CCTGGGAGCC ACCGTCTGTG ACCTCCGGCA AGATCATCGA GTACTCTGTG 
TACCTGGCCA TCCAGAGCTC ACAGGCCAGT GGTGAGCCAA AGAGCTCCAC CCCAGCCCAG 
CTGGCCTTCA TGCGAGTGTA CTGTGGGCCT AGCCCTTCCT GCCTAGTGCA GTCCTCCAGC 
CTCTCCAACG CCCACATTGA CTATACTACA AAGCCTGCCA TCATCTTCCG CATTGCTGCC 
CGCAATGAAA AGGGCTACGG CCCTGCCACA CAAGTGAGGT GGTTGCAAGA AACTAGTAAA 
GACAGCTCTG GCACCAAGCC GGCCAGCAAG CGGCCCATGT CGTCTCCAGA AATGAAATCT 
GCTCCAAAGA AGTCTAAGGC TGATGGTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcfc1 ( XM_006527836.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527899.1 Mus musculus host cell factor C1 (Hcfc1), transcript variant X2, mRNA.

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CloneID	OMu56158
Clone ID Related Accession (Same CDS sequence)	XM_006527836.1 , XM_006527836.2
Accession Version	XM_006527836.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCCATGGA TACATCTGAA GCAGCAGCAG CAGTGACACA AGCAGAACTG 
GGTCACCTTT CAGCTGAAGG CCAAGAGGGT CAGGCTACCA CCATACCCAT TGTGTTGACA 
CAGCAGGAGC TTGCAGCCCT GGTGCAGCAG CAGCAGCAGC TCCAGGAGGC TCAAGCTCAA 
GCCCAGCAAC AGCACCATCT TCCCACTGAG GCTCTGGCCC CAGCTGACAG TCTCAATGAC 
CCATCCATCG AGAGCAACTG CCTCAACGAG TTAGCTAGTG CTGTCCCAAG CACCGTGGCT 
TTGCTACCCT CAACAGCTAC CGAGAGCCTG GCTCCATCTA ACACATTTGT GGCTCCCCAG 
CCTGTTGTAG CTAGTCCAGC AAAGATGCAG GCTGCAGCTA CCCTTACTGA AGTGGCCAAT 
GGCATTGAGT CCCTGGGTGT GAAACCGGAC TTGCCACCCC CACCCAGCAA AGCCCCTGTG 
AAAAAGGAGA ACCAGTGGTT TGATGTGGGG GTCATTAAGG GTACCAGTGT AATGGTGACA 
CACTATTTTC TGCCACCAGA TGATGCTGTT CAGTCAGATG ATGACTCAGG CACGGTCCCA 
GACTATAACC AGCTAAAGAA GCAGGAGCTA CAGCCAGGCA CGGCTTACAA ATTTCGAGTT 
GCTGGAATCA ATGCTTGTGG CCGGGGACCC TTCAGTGAGA TCTCAGCCTT TAAGACTTGT 
CTGCCTGGGT TCCCAGGGGC TCCTTGTGCT ATTAAAATCA GCAAGAGCCC AGATGGTGCT 
CACCTCACCT GGGAGCCACC GTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCTGTGTAC 
CTGGCCATCC AGAGCTCACA GGCCAGTGGT GAGCCAAAGA GCTCCACCCC AGCCCAGCTG 
GCCTTCATGC GAGTGTACTG TGGGCCTAGC CCTTCCTGCC TAGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATTGACTA TACTACAAAG CCTGCCATCA TCTTCCGCAT TGCTGCCCGC 
AATGAAAAGG GCTACGGCCC TGCCACACAA GTGAGGTGGT TGCAAGAAAC TAGTAAAGAC 
AGCTCTGGCA CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCTGCT 
CCAAAGAAGT CTAAGGCTGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527899.1
CDS	1133..7402
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPNISSLTETTPGALNSEVPIPATITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006527836.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527836.1

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGAACATC TCATCATTGA CTGAGACTAC CCCAGGGGCT 
CTGAATTCCG AAGTCCCCAT CCCAGCCACG ATAACAGTGA CCATAGCCAA CACAGAAACT 
TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAACT CCAGGTCTCA 
CCAGGGCCTC GCCAGCAGCT GCCTCCACGG CAACTCCTGC AGTCTGCCTC CACACCCCTG 
ATGGGGGAGT CTACTGAGGT CCTGTCAGCC TCCCAGACCC CTGAGCTCCA GGCCGCCGTG 
GATCTGAGCA GCACTGGGGA CCCATCTTCA GGCCAGGAGC CTACCACCTC TGCTGTCGTG 
GCCACTGTGG TGGTCCAACC ACCCCCACCC ACACAGTCTG AAGTAGACCA GTTATCACTT 
CCCCAAGAGC TGATGGCTGA AGCCCAGGCG GGCACCACAA CCCTTATGGT AACAGGGCTC 
ACTCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCAG CTGCTCAAGC TGCAGCCACT 
GAAGAAGCCC AAGCCTTGGC CATCCAGGCT GTGCTCCAGG CCGCACAGCA GGCTGTCATG 
GGCACTGGGG AGCCCATGGA TACATCTGAA GCAGCAGCAG CAGTGACACA AGCAGAACTG 
GGTCACCTTT CAGCTGAAGG CCAAGAGGGT CAGGCTACCA CCATACCCAT TGTGTTGACA 
CAGCAGGAGC TTGCAGCCCT GGTGCAGCAG CAGCAGCAGC TCCAGGAGGC TCAAGCTCAA 
GCCCAGCAAC AGCACCATCT TCCCACTGAG GCTCTGGCCC CAGCTGACAG TCTCAATGAC 
CCATCCATCG AGAGCAACTG CCTCAACGAG TTAGCTAGTG CTGTCCCAAG CACCGTGGCT 
TTGCTACCCT CAACAGCTAC CGAGAGCCTG GCTCCATCTA ACACATTTGT GGCTCCCCAG 
CCTGTTGTAG CTAGTCCAGC AAAGATGCAG GCTGCAGCTA CCCTTACTGA AGTGGCCAAT 
GGCATTGAGT CCCTGGGTGT GAAACCGGAC TTGCCACCCC CACCCAGCAA AGCCCCTGTG 
AAAAAGGAGA ACCAGTGGTT TGATGTGGGG GTCATTAAGG GTACCAGTGT AATGGTGACA 
CACTATTTTC TGCCACCAGA TGATGCTGTT CAGTCAGATG ATGACTCAGG CACGGTCCCA 
GACTATAACC AGCTAAAGAA GCAGGAGCTA CAGCCAGGCA CGGCTTACAA ATTTCGAGTT 
GCTGGAATCA ATGCTTGTGG CCGGGGACCC TTCAGTGAGA TCTCAGCCTT TAAGACTTGT 
CTGCCTGGGT TCCCAGGGGC TCCTTGTGCT ATTAAAATCA GCAAGAGCCC AGATGGTGCT 
CACCTCACCT GGGAGCCACC GTCTGTGACC TCCGGCAAGA TCATCGAGTA CTCTGTGTAC 
CTGGCCATCC AGAGCTCACA GGCCAGTGGT GAGCCAAAGA GCTCCACCCC AGCCCAGCTG 
GCCTTCATGC GAGTGTACTG TGGGCCTAGC CCTTCCTGCC TAGTGCAGTC CTCCAGCCTC 
TCCAACGCCC ACATTGACTA TACTACAAAG CCTGCCATCA TCTTCCGCAT TGCTGCCCGC 
AATGAAAAGG GCTACGGCCC TGCCACACAA GTGAGGTGGT TGCAAGAAAC TAGTAAAGAC 
AGCTCTGGCA CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCTGCT 
CCAAAGAAGT CTAAGGCTGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcfc1 ( XM_017318387.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173876.1 Mus musculus host cell factor C1 (Hcfc1), transcript variant X3, mRNA.

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CloneID	OMu83077
Clone ID Related Accession (Same CDS sequence)	XM_017318387.2 , XM_017318387.1
Accession Version	XM_017318387.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGCCCCCA GAGGAACTCC AGGTCTCACC AGGGCCTCGC 
CAGCAGCTGC CTCCACGGCA ACTCCTGCAG TCTGCCTCCA CACCCCTGAT GGGGGAGTCT 
ACTGAGGTCC TGTCAGCCTC CCAGACCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGC 
ACTGGGGACC CATCTTCAGG CCAGGAGCCT ACCACCTCTG CTGTCGTGGC CACTGTGGTG 
GTCCAACCAC CCCCACCCAC ACAGTCTGAA GTAGACCAGT TATCACTTCC CCAAGAGCTG 
ATGGCTGAAG CCCAGGCGGG CACCACAACC CTTATGGTAA CAGGGCTCAC TCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCT GCTCAAGCTG CAGCCACTGA AGAAGCCCAA 
GCCTTGGCCA TCCAGGCTGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCCATGG ATACATCTGA AGCAGCAGCA GCAGTGACAC AAGCAGAACT GGGTCACCTT 
TCAGCTGAAG GCCAAGAGGG TCAGGCTACC ACCATACCCA TTGTGTTGAC ACAGCAGGAG 
CTTGCAGCCC TGGTGCAGCA GCAGCAGCAG CTCCAGGAGG CTCAAGCTCA AGCCCAGCAA 
CAGCACCATC TTCCCACTGA GGCTCTGGCC CCAGCTGACA GTCTCAATGA CCCATCCATC 
GAGAGCAACT GCCTCAACGA GTTAGCTAGT GCTGTCCCAA GCACCGTGGC TTTGCTACCC 
TCAACAGCTA CCGAGAGCCT GGCTCCATCT AACACATTTG TGGCTCCCCA GCCTGTTGTA 
GCTAGTCCAG CAAAGATGCA GGCTGCAGCT ACCCTTACTG AAGTGGCCAA TGGCATTGAG 
TCCCTGGGTG TGAAACCGGA CTTGCCACCC CCACCCAGCA AAGCCCCTGT GAAAAAGGAG 
AACCAGTGGT TTGATGTGGG GGTCATTAAG GGTACCAGTG TAATGGTGAC ACACTATTTT 
CTGCCACCAG ATGATGCTGT TCAGTCAGAT GATGACTCAG GCACGGTCCC AGACTATAAC 
CAGCTAAAGA AGCAGGAGCT ACAGCCAGGC ACGGCTTACA AATTTCGAGT TGCTGGAATC 
AATGCTTGTG GCCGGGGACC CTTCAGTGAG ATCTCAGCCT TTAAGACTTG TCTGCCTGGG 
TTCCCAGGGG CTCCTTGTGC TATTAAAATC AGCAAGAGCC CAGATGGTGC TCACCTCACC 
TGGGAGCCAC CGTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCTGTGTA CCTGGCCATC 
CAGAGCTCAC AGGCCAGTGG TGAGCCAAAG AGCTCCACCC CAGCCCAGCT GGCCTTCATG 
CGAGTGTACT GTGGGCCTAG CCCTTCCTGC CTAGTGCAGT CCTCCAGCCT CTCCAACGCC 
CACATTGACT ATACTACAAA GCCTGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAAAAG 
GGCTACGGCC CTGCCACACA AGTGAGGTGG TTGCAAGAAA CTAGTAAAGA CAGCTCTGGC 
ACCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCTGC TCCAAAGAAG 
TCTAAGGCTG ATGGTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173876.1
CDS	1133..7273
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGPLLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAARVVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGLEVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQGDSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTPLMGESTEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASAVPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_017318387.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus host cell factor C1 (Hcfc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318387.1

ORF Insert Sequence:

ATGGCTTCGG CTGTGTCTCC CGCAAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAA 
CGGGTGGTGG GCTGGTCGGG TCCCGTGCCC CGACCCCGCC ACGGCCACCG TGCAGTGGCT 
ATCAAGGAGC TTATAGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAACTACAC 
GTGTACAACA CTGCAACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA TATCCCTCCA 
GGGTGTGCAG CCTATGGCTT TGTGTGTGAT GGTACTCGCC TATTGGTGTT TGGTGGAATG 
GTAGAGTATG GAAAATACAG CAACGACCTC TATGAACTCC AGGCAAGTCG TTGGGAATGG 
AAGAGACTGA AGGCAAAGAC ACCCAAAAAT GGGCCTCCTC CATGTCCTCG GCTTGGACAT 
AGCTTCTCCC TTGTGGGCAA CAAATGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGTGAG 
GACCCCAAGA ACAACATTCC GAGGTACCTG AATGACTTAT ATATTCTCGA ACTACGGCCA 
GGCTCTGGAG TGGTAGCTTG GGACATCCCC ATCACTTACG GTGTCCTGCC TCCACCCCGG 
GAGTCACATA CTGCTGTGGT CTACACTGAA AAAGATAACA AGAAATCCAA GCTGGTGATC 
TATGGAGGGA TGAGTGGCTG CAGGCTAGGG GACCTTTGGA CCCTGGACAT TGAGACACTG 
ACATGGAATA AGCCCAGCCT TAGTGGGGTG GCACCCCTTC CTCGCAGCCT CCACTCTGCA 
ACCACCATAG GAAACAAAAT GTATGTATTT GGTGGCTGGG TGCCCCTTGT CATGGACGAT 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACACTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA AACCATCCTG ATGGATACAT TGGAGGACAA CATTCCTCGA 
GCTCGAGCAG GCCACTGTGC TGTTGCCATC AATACTCGTC TGTATATTTG GAGTGGCCGT 
GATGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACTTGTG GTATTTGGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTAGTAC GAGCCAACAC CAACTCACTG 
GAGGTTAGCT GGGGTGCAGT GGCAACAGCT GACAGTTACC TTCTACAACT CCAGAAATAT 
GACATTCCTG CCACAGCTGC TACGGCTACC TCCCCCACTC CCAATCCAGT CCCGTCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCGCCA GCAGCAGCTG CACCTGCTGT ACAGCCACTG 
ACCCAAGTAG GCATCACACT TGTGCCCCAG GCTGCCACTG CACCCCCAAG CACAACCACC 
ATCCAGGTCT TGCCGACAGT GCCAGGCAGC TCCATTTCTG TGCCCACTGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACT GGTCCTCAAG CTACAACAGG AACACCACTG 
GTTACCATGA GACCTGCAAG CCAGGCTGGA AAAGCTCCTG TCACTGTGAC TTCCCTGCCT 
GCCAGTGTTC GAATGGTTGT ACCCACACAG AGTGCCCAGG GGACGGTGAT CGGCAGCAAC 
CCACAGATGA GTGGGATGGC CGCATTGGCT GCTGCTGCTG CTGCCACACA GAAAATCCCT 
CCATCCTCAG CACCCACGGT GCTGAGTGTC CCAGCAGGGA CCACCATCGT CAAGACAGTG 
GCTGTGACAC CTGGCACGAC CACTCTTCCA GCCACTGTGA AGGTGGCCTC CTCCCCTGTC 
ATGGTGAGCA ACCCAGCCAC TCGAATGCTA AAGACTGCAG CTGCCCAAGT GGGGACATCT 
GTGTCCTCTG CTGCCAACAC ATCTACTCGC CCTATCATCA CAGTACACAA ATCAGGAACT 
GTAACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACGGTGG TAGGTGGAGT CACCAAGACC 
ATCACCCTAG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGAAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CATCAGCAGT GACAGGCCAA 
GCATCTACAG GTCCTGTGAC TCAGATCATC CAGACCAAAG GACCCCTGCC AGCGGGGACT 
ATCCTGAAGC TGGTGACATC AGCAGATGGC AAGCCCACAA CCATCATTAC CACCACACAG 
GCTAGTGGGG CAGGGACCAA GCCCACTATC CTGGGCATCA GTAGTGTTTC TCCCAGCACC 
ACCAAACCTG GCACAACTAC CATTATTAAG ACCATTCCTA TGTCGGCCAT TATCACCCAG 
GCAGGTGCCA CAGGTGTTAC CAGCAGTCCT GGCATTAAGT CCCCAATTAC AATTATCACC 
ACCAAAGTGA TGACTTCAGG AACAGGAGCG CCTGCTAAAA TCATCACTGC TGTCCCCAAG 
ATTGCTACTG GCCATGGGCA ACAAGGAGTG ACCCAGGTGG TGCTAAAGGG GGCCCCTGGA 
CAACCAGGCA CCATCCTCCG TACTGTGCCT ATGGGCGGCG TTCGCCTGGT CACCCCTGTC 
ACCGTCTCTG CTGTCAAGCC AGCTGTCACC ACATTGGTTG TGAAGGGTAC CACAGGTGTT 
ACAACGCTAG GCACAGTGAC AGGCACTGTC TCCACCAGCC TGGCCGGAGC TGGGGCACAT 
AGCACCAGTG CTTCCCTGGC TACACCTATC ACTACCTTGG GCACTATTGC TACGCTCTCA 
AGCCAGGTGA TCAACCCTAC TGCTATCACA GTGTCAGCTG CACAGACTAC ACTAACAGCT 
GCTGGTGGGC TTACCACACC CACAATCACA ATGCAGCCTG TCTCCCAGCC TACCCAGGTC 
ACTCTGATTA CAGCACCCAG TGGGGTTGAA GCACAGCCTG TACATGACCT TCCTGTATCC 
ATTTTGGCCT CACCTACTAC AGAGCAGCCC ACAGCAACAG TCACCATCGC TGACTCAGGC 
CAGGGTGATG TGCAGCCCGG CACTGTGACA CTGGTGTGTT CCAACCCACC CTGTGAAACC 
CATGAAACAG GCACCACCAA CACAGCTACC ACCACTGTTG TGGCTAACCT TGGGGGACAT 
CCTCAACCTA CCCAGGTGCA GTTTGTTTGT GACAGACAGG AGACAGCTGC TTCACTTGTG 
ACCTCAGCTG TAGGACAACA GAATGGTAAT GTGGTCCGTG TCTGTTCAAA CCCCCCCTGT 
GAGACCCATG AGACGGGCAC TACCAACACT GCCACAACAG CCACCTCCAA CATGGCTGGG 
CAGCATGGCT GCTCGAACCC CCCCTGTGAG ACTCATGAGA CAGGCACCAC CAGCACTGCC 
ACTACAGCAA TGTCCAGCAT GGGCACTGGG CAGCAGCGAG ACACTCGTCG TACCACTAAC 
ACCCCCACTG TAGTGCGGAT CACTGTGGCT CCTGGGGCAT TGGAGAGAGT CCAGGGTACC 
GTGAAGCCTC AGTGCCAAAC CCAGCAGACC AACATGACCA CCACCACCAT GACTGTGCAG 
GCCACTGGAG CTCCATGCTC AGCTGGCCCC CTGCTTAGGC CAAGTGTGGC ACTGGAGTCT 
GGGAGCCACA GCCCTGCCTT TGTGCAACTA GCCCTTCCAA GTGTCAGAGT TGGGCTAAGT 
GGCCCCAGCA GCAAGGACAT GCCCACAGGG CGCCAACCAG AGACATATCA TACTTACACA 
ACTAATACCC CAACCACAAC CCGCTCTATC ATGGTTGCTG GGGAGCTTGG TGCAGCTCGG 
GTGGTCCCCA CATCTACATA TGAGAGCCTC CAGGCAAGCT CTCCTAGCAG CACCATGACT 
ATGACAGCCC TAGAGGCACT GCTGTGCCCT TCGGCTACTG TCACCCAAGT CTGCTCCAAC 
CCGCCATGTG AGACCCATGA GACGGGTACC ACCAACACCG CCACTACCTC CAATGCGGGC 
AGTGCTCAGC GAGTATGCTC CAACCCGCCT TGTGAGACTC ATGAGACGGG CACCACACAC 
ACAGCTACCA CTGCCACATC AAATGGAGGT GCAGGCCAGC CTGAGGGTGG ACAACAGCCT 
GCCAGTGGCC ATCCCTGCGA GACACACCAG ACCACTTCCA CTGGCACCAC TATGTCAGTC 
AGTGTGGGTA CCCTGATTCC TGATGCTACT TCCTCTCATG GAACCCTGGA GTCGGGCTTA 
GAGGTGGTAG CAGTGCCCAC TGTCACCTCC CAGGCTGGTT CCACATTGCT GGCCTCTTTC 
CCAACACAGA GGGTATGCTC CAACCCTCCT TGCGAGACCC ACGAGACAGG TACCACGCAC 
ACAGCCACCA CTGTCACCTC TAACATGAGC TCAAACCAAG ACCCTCCACC AGCTGCCAGT 
GACCAAGGAG AGGTGGCAAG CACCCAAGGT GACAGCACAA ATATCACCAG TGCCAGTGCT 
ATCACTACAA GTGTGTCTTC TACATTGCCA CGAGCAGTGA CCACTGTGAC ACAGTCTACA 
CCAGTCCCTG GTCCCTCTGT GCCGCCCCCA GAGGAACTCC AGGTCTCACC AGGGCCTCGC 
CAGCAGCTGC CTCCACGGCA ACTCCTGCAG TCTGCCTCCA CACCCCTGAT GGGGGAGTCT 
ACTGAGGTCC TGTCAGCCTC CCAGACCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGC 
ACTGGGGACC CATCTTCAGG CCAGGAGCCT ACCACCTCTG CTGTCGTGGC CACTGTGGTG 
GTCCAACCAC CCCCACCCAC ACAGTCTGAA GTAGACCAGT TATCACTTCC CCAAGAGCTG 
ATGGCTGAAG CCCAGGCGGG CACCACAACC CTTATGGTAA CAGGGCTCAC TCCAGAGGAG 
CTGGCAGTGA CTGCTGCTGC TGAAGCAGCT GCTCAAGCTG CAGCCACTGA AGAAGCCCAA 
GCCTTGGCCA TCCAGGCTGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACTGGG 
GAGCCCATGG ATACATCTGA AGCAGCAGCA GCAGTGACAC AAGCAGAACT GGGTCACCTT 
TCAGCTGAAG GCCAAGAGGG TCAGGCTACC ACCATACCCA TTGTGTTGAC ACAGCAGGAG 
CTTGCAGCCC TGGTGCAGCA GCAGCAGCAG CTCCAGGAGG CTCAAGCTCA AGCCCAGCAA 
CAGCACCATC TTCCCACTGA GGCTCTGGCC CCAGCTGACA GTCTCAATGA CCCATCCATC 
GAGAGCAACT GCCTCAACGA GTTAGCTAGT GCTGTCCCAA GCACCGTGGC TTTGCTACCC 
TCAACAGCTA CCGAGAGCCT GGCTCCATCT AACACATTTG TGGCTCCCCA GCCTGTTGTA 
GCTAGTCCAG CAAAGATGCA GGCTGCAGCT ACCCTTACTG AAGTGGCCAA TGGCATTGAG 
TCCCTGGGTG TGAAACCGGA CTTGCCACCC CCACCCAGCA AAGCCCCTGT GAAAAAGGAG 
AACCAGTGGT TTGATGTGGG GGTCATTAAG GGTACCAGTG TAATGGTGAC ACACTATTTT 
CTGCCACCAG ATGATGCTGT TCAGTCAGAT GATGACTCAG GCACGGTCCC AGACTATAAC 
CAGCTAAAGA AGCAGGAGCT ACAGCCAGGC ACGGCTTACA AATTTCGAGT TGCTGGAATC 
AATGCTTGTG GCCGGGGACC CTTCAGTGAG ATCTCAGCCT TTAAGACTTG TCTGCCTGGG 
TTCCCAGGGG CTCCTTGTGC TATTAAAATC AGCAAGAGCC CAGATGGTGC TCACCTCACC 
TGGGAGCCAC CGTCTGTGAC CTCCGGCAAG ATCATCGAGT ACTCTGTGTA CCTGGCCATC 
CAGAGCTCAC AGGCCAGTGG TGAGCCAAAG AGCTCCACCC CAGCCCAGCT GGCCTTCATG 
CGAGTGTACT GTGGGCCTAG CCCTTCCTGC CTAGTGCAGT CCTCCAGCCT CTCCAACGCC 
CACATTGACT ATACTACAAA GCCTGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAAAAG 
GGCTACGGCC CTGCCACACA AGTGAGGTGG TTGCAAGAAA CTAGTAAAGA CAGCTCTGGC 
ACCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCTGC TCCAAAGAAG 
TCTAAGGCTG ATGGTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Scientific reports637396(2016 Nov)
Cui W,Dai X,Marcho C,Han Z,Zhang K,Tremblay KD,Mager J

Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification.
Developmental biology418(1)75-88(2016 10)
Minocha S,Bessonnard S,Sung TL,Moret C,Constam DB,Herr W

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Hcfc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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