TSC22D1 cDNA ORF clone, Bos taurus(cattle)

The following TSC22D1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC22D1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa24657 XM_024999842.1
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OBa24657 XM_005213608.4
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OBa42631 NM_001034377.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OBa07210 XM_005213605.3
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa07210 XM_005194095.3
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24656 XM_005213606.4
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47652 XM_010810694.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47652 XM_010820115.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47653 XM_010810695.3
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47653 XM_010820124.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24655 XM_005213604.4
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47649 XM_010810690.3
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47649 XM_010820101.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47650 XM_010820106.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47650 XM_010810691.3
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47651 XM_010810692.3
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47651 XM_024999840.1
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47651 XM_010820109.2
Latest version!
Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa47653 XM_010810695.2 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24657 XM_005213608.3 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OBa47649 XM_010810690.2 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47651 XM_010810692.2 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa24655 XM_005213604.3 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa42631 NM_001034377.1 Bos taurus TSC22 domain family member 1 (TSC22D1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OBa24656 XM_005213606.3 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47650 XM_010810691.2 Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa42631
    Clone ID Related Accession (Same CDS sequence) NM_001034377.2 , NM_001034377.1
    Accession Version NM_001034377.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-14
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105192.1. On or before Oct 17, 2005 this sequence version replaced XM_587491.2, XM_872160.1, XM_864139.1. ##Evidence-Data-START## Transcript exon combination :: BC105192.1, DV893957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145424 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATCCC AATGGTGTAG ACCAGTGGCG ATGGATCTAG GAGTTTACCA ACTGAGACAT 
    TTTTCAATTT CTTTCTTGTC ATCCTTGCTC GGGACCGAAA ACGCCTCTGT GAGACTTGAC
    AATAGCTCAT CTGGTGCAAG TGTGGTAGCT ATTGACAACA AAATCGAGCA AGCTATGGAT
    CTGGTGAAAA GCCATTTGAT GTATGCAGTT AGAGAGGAAG TGGAGGTCCT CAAGGAACAG
    ATCAAGGAAC TCATAGAGAA AAATTCCCAG CTGGAGCAGG AGAACAATCT GCTGAAGACA
    CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC CAGGCCCAGC TGCAGACTGG CTCGCCCCCT
    GCCACCACAC AGCCACAGGG GACCACACAG CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA
    GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001029549.1
    CDS239..673
    Translation

    Target ORF information:

    RefSeq Version NM_001034377.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001034377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATCCC AATGGTGTAG ACCAGTGGCG ATGGATCTAG GAGTTTACCA ACTGAGACAT 
    TTTTCAATTT CTTTCTTGTC ATCCTTGCTC GGGACCGAAA ACGCCTCTGT GAGACTTGAC
    AATAGCTCAT CTGGTGCAAG TGTGGTAGCT ATTGACAACA AAATCGAGCA AGCTATGGAT
    CTGGTGAAAA GCCATTTGAT GTATGCAGTT AGAGAGGAAG TGGAGGTCCT CAAGGAACAG
    ATCAAGGAAC TCATAGAGAA AAATTCCCAG CTGGAGCAGG AGAACAATCT GCTGAAGACA
    CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC CAGGCCCAGC TGCAGACTGG CTCGCCCCCT
    GCCACCACAC AGCCACAGGG GACCACACAG CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA
    GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24657
    Clone ID Related Accession (Same CDS sequence) XM_005213608.3 , XM_005213608.4 , XM_024999842.1
    Accession Version XM_005213608.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213608.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG AGGAAGTGGA GGTCCTCAAG 
    GAACAGATCA AGGAACTCAT AGAGAAAAAT TCCCAGCTGG AGCAGGAGAA CAATCTGCTG
    AAGACACTGG CCAGTCCCGA GCAGCTTGCC CAGTTCCAGG CCCAGCTGCA GACTGGCTCG
    CCCCCTGCCA CCACACAGCC ACAGGGGACC ACACAGCCCC CAGCCCAGCC AGCGTCCCAG
    GGCTCAGGAC CAACCGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213665.1
    CDS101..361
    Translation

    Target ORF information:

    RefSeq Version XM_005213608.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213608.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG AGGAAGTGGA GGTCCTCAAG 
    GAACAGATCA AGGAACTCAT AGAGAAAAAT TCCCAGCTGG AGCAGGAGAA CAATCTGCTG
    AAGACACTGG CCAGTCCCGA GCAGCTTGCC CAGTTCCAGG CCCAGCTGCA GACTGGCTCG
    CCCCCTGCCA CCACACAGCC ACAGGGGACC ACACAGCCCC CAGCCCAGCC AGCGTCCCAG
    GGCTCAGGAC CAACCGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24657
    Clone ID Related Accession (Same CDS sequence) XM_005213608.3 , XM_005213608.4 , XM_024999842.1
    Accession Version XM_005213608.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213608.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG AGGAAGTGGA GGTCCTCAAG 
    GAACAGATCA AGGAACTCAT AGAGAAAAAT TCCCAGCTGG AGCAGGAGAA CAATCTGCTG
    AAGACACTGG CCAGTCCCGA GCAGCTTGCC CAGTTCCAGG CCCAGCTGCA GACTGGCTCG
    CCCCCTGCCA CCACACAGCC ACAGGGGACC ACACAGCCCC CAGCCCAGCC AGCGTCCCAG
    GGCTCAGGAC CAACCGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213665.1
    CDS108..368
    Translation

    Target ORF information:

    RefSeq Version XM_005213608.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213608.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG AGGAAGTGGA GGTCCTCAAG 
    GAACAGATCA AGGAACTCAT AGAGAAAAAT TCCCAGCTGG AGCAGGAGAA CAATCTGCTG
    AAGACACTGG CCAGTCCCGA GCAGCTTGCC CAGTTCCAGG CCCAGCTGCA GACTGGCTCG
    CCCCCTGCCA CCACACAGCC ACAGGGGACC ACACAGCCCC CAGCCCAGCC AGCGTCCCAG
    GGCTCAGGAC CAACCGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24657
    Clone ID Related Accession (Same CDS sequence) XM_005213608.3 , XM_005213608.4 , XM_024999842.1
    Accession Version XM_024999842.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG AGGAAGTGGA GGTCCTCAAG 
    GAACAGATCA AGGAACTCAT AGAGAAAAAT TCCCAGCTGG AGCAGGAGAA CAATCTGCTG
    AAGACACTGG CCAGTCCCGA GCAGCTTGCC CAGTTCCAGG CCCAGCTGCA GACTGGCTCG
    CCCCCTGCCA CCACACAGCC ACAGGGGACC ACACAGCCCC CAGCCCAGCC AGCGTCCCAG
    GGCTCAGGAC CAACCGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855610.1
    CDS173..433
    Translation

    Target ORF information:

    RefSeq Version XM_024999842.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG AGGAAGTGGA GGTCCTCAAG 
    GAACAGATCA AGGAACTCAT AGAGAAAAAT TCCCAGCTGG AGCAGGAGAA CAATCTGCTG
    AAGACACTGG CCAGTCCCGA GCAGCTTGCC CAGTTCCAGG CCCAGCTGCA GACTGGCTCG
    CCCCCTGCCA CCACACAGCC ACAGGGGACC ACACAGCCCC CAGCCCAGCC AGCGTCCCAG
    GGCTCAGGAC CAACCGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa42631
    Clone ID Related Accession (Same CDS sequence) NM_001034377.2 , NM_001034377.1
    Accession Version NM_001034377.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC105192.1. On Aug 22, 2019 this sequence version replaced NM_001034377.1. ##Evidence-Data-START## Transcript exon combination :: BC105192.1, SRR5571265.7837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145424 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATCCC AATGGTGTAG ACCAGTGGCG ATGGATCTAG GAGTTTACCA ACTGAGACAT 
    TTTTCAATTT CTTTCTTGTC ATCCTTGCTC GGGACCGAAA ACGCCTCTGT GAGACTTGAC
    AATAGCTCAT CTGGTGCAAG TGTGGTAGCT ATTGACAACA AAATCGAGCA AGCTATGGAT
    CTGGTGAAAA GCCATTTGAT GTATGCAGTT AGAGAGGAAG TGGAGGTCCT CAAGGAACAG
    ATCAAGGAAC TCATAGAGAA AAATTCCCAG CTGGAGCAGG AGAACAATCT GCTGAAGACA
    CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC CAGGCCCAGC TGCAGACTGG CTCGCCCCCT
    GCCACCACAC AGCCACAGGG GACCACACAG CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA
    GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001029549.1
    CDS238..672
    Translation

    Target ORF information:

    RefSeq Version NM_001034377.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001034377.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATCCC AATGGTGTAG ACCAGTGGCG ATGGATCTAG GAGTTTACCA ACTGAGACAT 
    TTTTCAATTT CTTTCTTGTC ATCCTTGCTC GGGACCGAAA ACGCCTCTGT GAGACTTGAC
    AATAGCTCAT CTGGTGCAAG TGTGGTAGCT ATTGACAACA AAATCGAGCA AGCTATGGAT
    CTGGTGAAAA GCCATTTGAT GTATGCAGTT AGAGAGGAAG TGGAGGTCCT CAAGGAACAG
    ATCAAGGAAC TCATAGAGAA AAATTCCCAG CTGGAGCAGG AGAACAATCT GCTGAAGACA
    CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC CAGGCCCAGC TGCAGACTGG CTCGCCCCCT
    GCCACCACAC AGCCACAGGG GACCACACAG CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA
    GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07210
    Clone ID Related Accession (Same CDS sequence) XM_005213605.3 , XM_005194095.3
    Accession Version XM_005213605.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213605.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTGTT GGCATTTTTC CAAACTGGCC
    GGCAGAGCCA GGAGGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213662.1
    CDS358..3258
    Translation

    Target ORF information:

    RefSeq Version XM_005213605.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213605.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTGTT GGCATTTTTC CAAACTGGCC
    GGCAGAGCCA GGAGGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07210
    Clone ID Related Accession (Same CDS sequence) XM_005213605.3 , XM_005194095.3
    Accession Version XM_005194095.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTGTT GGCATTTTTC CAAACTGGCC
    GGCAGAGCCA GGAGGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194152.1
    CDS358..3258
    Translation

    Target ORF information:

    RefSeq Version XM_005194095.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194095.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTGTT GGCATTTTTC CAAACTGGCC
    GGCAGAGCCA GGAGGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24656
    Clone ID Related Accession (Same CDS sequence) XM_005213606.4 , XM_005213606.3
    Accession Version XM_005213606.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213606.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGCTCATCTG GTGCAAGTGT GGTAGCTATT
    GACAACAAAA TCGAGCAAGC TATGGATCTG GTGAAAAGCC ATTTGATGTA TGCAGTTAGA
    GAGGAAGTGG AGGTCCTCAA GGAACAGATC AAGGAACTCA TAGAGAAAAA TTCCCAGCTG
    GAGCAGGAGA ACAATCTGCT GAAGACACTG GCCAGTCCCG AGCAGCTTGC CCAGTTCCAG
    GCCCAGCTGC AGACTGGCTC GCCCCCTGCC ACCACACAGC CACAGGGGAC CACACAGCCC
    CCAGCCCAGC CAGCGTCCCA GGGCTCAGGA CCAACCGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213663.1
    CDS611..3412
    Translation

    Target ORF information:

    RefSeq Version XM_005213606.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213606.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGCTCATCTG GTGCAAGTGT GGTAGCTATT
    GACAACAAAA TCGAGCAAGC TATGGATCTG GTGAAAAGCC ATTTGATGTA TGCAGTTAGA
    GAGGAAGTGG AGGTCCTCAA GGAACAGATC AAGGAACTCA TAGAGAAAAA TTCCCAGCTG
    GAGCAGGAGA ACAATCTGCT GAAGACACTG GCCAGTCCCG AGCAGCTTGC CCAGTTCCAG
    GCCCAGCTGC AGACTGGCTC GCCCCCTGCC ACCACACAGC CACAGGGGAC CACACAGCCC
    CCAGCCCAGC CAGCGTCCCA GGGCTCAGGA CCAACCGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24656
    Clone ID Related Accession (Same CDS sequence) XM_005213606.4 , XM_005213606.3
    Accession Version XM_005213606.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGCTCATCTG GTGCAAGTGT GGTAGCTATT
    GACAACAAAA TCGAGCAAGC TATGGATCTG GTGAAAAGCC ATTTGATGTA TGCAGTTAGA
    GAGGAAGTGG AGGTCCTCAA GGAACAGATC AAGGAACTCA TAGAGAAAAA TTCCCAGCTG
    GAGCAGGAGA ACAATCTGCT GAAGACACTG GCCAGTCCCG AGCAGCTTGC CCAGTTCCAG
    GCCCAGCTGC AGACTGGCTC GCCCCCTGCC ACCACACAGC CACAGGGGAC CACACAGCCC
    CCAGCCCAGC CAGCGTCCCA GGGCTCAGGA CCAACCGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213663.1
    CDS346..3147
    Translation

    Target ORF information:

    RefSeq Version XM_005213606.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213606.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGCTCATCTG GTGCAAGTGT GGTAGCTATT
    GACAACAAAA TCGAGCAAGC TATGGATCTG GTGAAAAGCC ATTTGATGTA TGCAGTTAGA
    GAGGAAGTGG AGGTCCTCAA GGAACAGATC AAGGAACTCA TAGAGAAAAA TTCCCAGCTG
    GAGCAGGAGA ACAATCTGCT GAAGACACTG GCCAGTCCCG AGCAGCTTGC CCAGTTCCAG
    GCCCAGCTGC AGACTGGCTC GCCCCCTGCC ACCACACAGC CACAGGGGAC CACACAGCCC
    CCAGCCCAGC CAGCGTCCCA GGGCTCAGGA CCAACCGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47652
    Clone ID Related Accession (Same CDS sequence) XM_010820115.2 , XM_010810694.2
    Accession Version XM_010820115.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCTC TATTTCAGTG CTTCTCTCCT
    ACCAGAGGTG CAAGGAGTGA TCCTAGAACC ACAGATACAG CCAAGACCAC GGAGAGCTTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818417.1
    CDS358..3300
    Translation

    Target ORF information:

    RefSeq Version XM_010820115.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820115.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCTC TATTTCAGTG CTTCTCTCCT
    ACCAGAGGTG CAAGGAGTGA TCCTAGAACC ACAGATACAG CCAAGACCAC GGAGAGCTTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47652
    Clone ID Related Accession (Same CDS sequence) XM_010820115.2 , XM_010810694.2
    Accession Version XM_010810694.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCTC TATTTCAGTG CTTCTCTCCT
    ACCAGAGGTG CAAGGAGTGA TCCTAGAACC ACAGATACAG CCAAGACCAC GGAGAGCTTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808996.1
    CDS358..3300
    Translation

    Target ORF information:

    RefSeq Version XM_010810694.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810694.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCTC TATTTCAGTG CTTCTCTCCT
    ACCAGAGGTG CAAGGAGTGA TCCTAGAACC ACAGATACAG CCAAGACCAC GGAGAGCTTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47653
    Clone ID Related Accession (Same CDS sequence) XM_010810695.3 , XM_010810695.2 , XM_010820124.2
    Accession Version XM_010810695.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGTGCTTCTC TCCTACCAGA GGTGCAAGGA
    GTGATCCTAG AACCACAGAT ACAGCCAAGA CCACGGAGAG CTTTTGACGT CAGGGGTCCA
    CTTTCTCCAC TGAACCTTTG GAGGCAGAAT ATCCAGCTTC TGGAGAGAGT TGGGAAAGGA
    TTTCTTTCAA CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAGC AGAGCCTTCA
    AGGATGATAT CTGTCACACA TGCAGCAACT GCAGTTTGGA AGGCAGTGGT GAACGGAGCC
    ATGCACTATG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808997.1
    CDS635..3409
    Translation

    Target ORF information:

    RefSeq Version XM_010810695.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810695.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGTGCTTCTC TCCTACCAGA GGTGCAAGGA
    GTGATCCTAG AACCACAGAT ACAGCCAAGA CCACGGAGAG CTTTTGACGT CAGGGGTCCA
    CTTTCTCCAC TGAACCTTTG GAGGCAGAAT ATCCAGCTTC TGGAGAGAGT TGGGAAAGGA
    TTTCTTTCAA CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAGC AGAGCCTTCA
    AGGATGATAT CTGTCACACA TGCAGCAACT GCAGTTTGGA AGGCAGTGGT GAACGGAGCC
    ATGCACTATG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47653
    Clone ID Related Accession (Same CDS sequence) XM_010810695.3 , XM_010810695.2 , XM_010820124.2
    Accession Version XM_010810695.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGTGCTTCTC TCCTACCAGA GGTGCAAGGA
    GTGATCCTAG AACCACAGAT ACAGCCAAGA CCACGGAGAG CTTTTGACGT CAGGGGTCCA
    CTTTCTCCAC TGAACCTTTG GAGGCAGAAT ATCCAGCTTC TGGAGAGAGT TGGGAAAGGA
    TTTCTTTCAA CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAGC AGAGCCTTCA
    AGGATGATAT CTGTCACACA TGCAGCAACT GCAGTTTGGA AGGCAGTGGT GAACGGAGCC
    ATGCACTATG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808997.1
    CDS361..3135
    Translation

    Target ORF information:

    RefSeq Version XM_010810695.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGTGCTTCTC TCCTACCAGA GGTGCAAGGA
    GTGATCCTAG AACCACAGAT ACAGCCAAGA CCACGGAGAG CTTTTGACGT CAGGGGTCCA
    CTTTCTCCAC TGAACCTTTG GAGGCAGAAT ATCCAGCTTC TGGAGAGAGT TGGGAAAGGA
    TTTCTTTCAA CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAGC AGAGCCTTCA
    AGGATGATAT CTGTCACACA TGCAGCAACT GCAGTTTGGA AGGCAGTGGT GAACGGAGCC
    ATGCACTATG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47653
    Clone ID Related Accession (Same CDS sequence) XM_010810695.3 , XM_010810695.2 , XM_010820124.2
    Accession Version XM_010820124.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGTGCTTCTC TCCTACCAGA GGTGCAAGGA
    GTGATCCTAG AACCACAGAT ACAGCCAAGA CCACGGAGAG CTTTTGACGT CAGGGGTCCA
    CTTTCTCCAC TGAACCTTTG GAGGCAGAAT ATCCAGCTTC TGGAGAGAGT TGGGAAAGGA
    TTTCTTTCAA CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAGC AGAGCCTTCA
    AGGATGATAT CTGTCACACA TGCAGCAACT GCAGTTTGGA AGGCAGTGGT GAACGGAGCC
    ATGCACTATG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818426.1
    CDS361..3135
    Translation

    Target ORF information:

    RefSeq Version XM_010820124.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820124.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT GCTACCGCTG
    ACGACACCCC TGGTGGATGG CGAGGACGAG AGTGCTTCTC TCCTACCAGA GGTGCAAGGA
    GTGATCCTAG AACCACAGAT ACAGCCAAGA CCACGGAGAG CTTTTGACGT CAGGGGTCCA
    CTTTCTCCAC TGAACCTTTG GAGGCAGAAT ATCCAGCTTC TGGAGAGAGT TGGGAAAGGA
    TTTCTTTCAA CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAGC AGAGCCTTCA
    AGGATGATAT CTGTCACACA TGCAGCAACT GCAGTTTGGA AGGCAGTGGT GAACGGAGCC
    ATGCACTATG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24655
    Clone ID Related Accession (Same CDS sequence) XM_005213604.4 , XM_005213604.3
    Accession Version XM_005213604.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213604.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCAT CTGGTGCAAG TGTGGTAGCT
    ATTGACAACA AAATCGAGCA AGCTATGGAT CTGGTGAAAA GCCATTTGAT GTATGCAGTT
    AGAGAGGAAG TGGAGGTCCT CAAGGAACAG ATCAAGGAAC TCATAGAGAA AAATTCCCAG
    CTGGAGCAGG AGAACAATCT GCTGAAGACA CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC
    CAGGCCCAGC TGCAGACTGG CTCGCCCCCT GCCACCACAC AGCCACAGGG GACCACACAG
    CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213661.1
    CDS1160..4324
    Translation

    Target ORF information:

    RefSeq Version XM_005213604.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213604.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCAT CTGGTGCAAG TGTGGTAGCT
    ATTGACAACA AAATCGAGCA AGCTATGGAT CTGGTGAAAA GCCATTTGAT GTATGCAGTT
    AGAGAGGAAG TGGAGGTCCT CAAGGAACAG ATCAAGGAAC TCATAGAGAA AAATTCCCAG
    CTGGAGCAGG AGAACAATCT GCTGAAGACA CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC
    CAGGCCCAGC TGCAGACTGG CTCGCCCCCT GCCACCACAC AGCCACAGGG GACCACACAG
    CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24655
    Clone ID Related Accession (Same CDS sequence) XM_005213604.4 , XM_005213604.3
    Accession Version XM_005213604.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213604.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCAT CTGGTGCAAG TGTGGTAGCT
    ATTGACAACA AAATCGAGCA AGCTATGGAT CTGGTGAAAA GCCATTTGAT GTATGCAGTT
    AGAGAGGAAG TGGAGGTCCT CAAGGAACAG ATCAAGGAAC TCATAGAGAA AAATTCCCAG
    CTGGAGCAGG AGAACAATCT GCTGAAGACA CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC
    CAGGCCCAGC TGCAGACTGG CTCGCCCCCT GCCACCACAC AGCCACAGGG GACCACACAG
    CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213661.1
    CDS347..3511
    Translation

    Target ORF information:

    RefSeq Version XM_005213604.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213604.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGCTCAT CTGGTGCAAG TGTGGTAGCT
    ATTGACAACA AAATCGAGCA AGCTATGGAT CTGGTGAAAA GCCATTTGAT GTATGCAGTT
    AGAGAGGAAG TGGAGGTCCT CAAGGAACAG ATCAAGGAAC TCATAGAGAA AAATTCCCAG
    CTGGAGCAGG AGAACAATCT GCTGAAGACA CTGGCCAGTC CCGAGCAGCT TGCCCAGTTC
    CAGGCCCAGC TGCAGACTGG CTCGCCCCCT GCCACCACAC AGCCACAGGG GACCACACAG
    CCCCCAGCCC AGCCAGCGTC CCAGGGCTCA GGACCAACCG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47649
    Clone ID Related Accession (Same CDS sequence) XM_010820101.2 , XM_010810690.3 , XM_010810690.2
    Accession Version XM_010820101.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGATTTCTTT CAACTGGTAA ACATCCTCCT TCCGCCAAAG GAACTCCTCT AGCAGAGCCT
    TCAAGGATGA TATCTGTCAC ACATGCAGCA ACTGCAGTTT GGAAGGCAGT GGTGAACGGA
    GCCATGCACT ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818403.1
    CDS359..3496
    Translation

    Target ORF information:

    RefSeq Version XM_010820101.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820101.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGATTTCTTT CAACTGGTAA ACATCCTCCT TCCGCCAAAG GAACTCCTCT AGCAGAGCCT
    TCAAGGATGA TATCTGTCAC ACATGCAGCA ACTGCAGTTT GGAAGGCAGT GGTGAACGGA
    GCCATGCACT ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47649
    Clone ID Related Accession (Same CDS sequence) XM_010820101.2 , XM_010810690.3 , XM_010810690.2
    Accession Version XM_010810690.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGATTTCTTT CAACTGGTAA ACATCCTCCT TCCGCCAAAG GAACTCCTCT AGCAGAGCCT
    TCAAGGATGA TATCTGTCAC ACATGCAGCA ACTGCAGTTT GGAAGGCAGT GGTGAACGGA
    GCCATGCACT ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808992.1
    CDS633..3770
    Translation

    Target ORF information:

    RefSeq Version XM_010810690.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810690.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGATTTCTTT CAACTGGTAA ACATCCTCCT TCCGCCAAAG GAACTCCTCT AGCAGAGCCT
    TCAAGGATGA TATCTGTCAC ACATGCAGCA ACTGCAGTTT GGAAGGCAGT GGTGAACGGA
    GCCATGCACT ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47649
    Clone ID Related Accession (Same CDS sequence) XM_010820101.2 , XM_010810690.3 , XM_010810690.2
    Accession Version XM_010810690.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGATTTCTTT CAACTGGTAA ACATCCTCCT TCCGCCAAAG GAACTCCTCT AGCAGAGCCT
    TCAAGGATGA TATCTGTCAC ACATGCAGCA ACTGCAGTTT GGAAGGCAGT GGTGAACGGA
    GCCATGCACT ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808992.1
    CDS359..3496
    Translation

    Target ORF information:

    RefSeq Version XM_010810690.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGATTTCTTT CAACTGGTAA ACATCCTCCT TCCGCCAAAG GAACTCCTCT AGCAGAGCCT
    TCAAGGATGA TATCTGTCAC ACATGCAGCA ACTGCAGTTT GGAAGGCAGT GGTGAACGGA
    GCCATGCACT ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47650
    Clone ID Related Accession (Same CDS sequence) XM_010810691.2 , XM_010820106.2 , XM_010810691.3
    Accession Version XM_010810691.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTATCTTG ATAATGGGAG ACCGGTTTAT TTGTTCTCTA ATTGCCCAGA TTTCTTTCAA
    CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808993.1
    CDS359..3457
    Translation

    Target ORF information:

    RefSeq Version XM_010810691.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTATCTTG ATAATGGGAG ACCGGTTTAT TTGTTCTCTA ATTGCCCAGA TTTCTTTCAA
    CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47650
    Clone ID Related Accession (Same CDS sequence) XM_010810691.2 , XM_010820106.2 , XM_010810691.3
    Accession Version XM_010820106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTATCTTG ATAATGGGAG ACCGGTTTAT TTGTTCTCTA ATTGCCCAGA TTTCTTTCAA
    CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818408.1
    CDS359..3457
    Translation

    Target ORF information:

    RefSeq Version XM_010820106.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTATCTTG ATAATGGGAG ACCGGTTTAT TTGTTCTCTA ATTGCCCAGA TTTCTTTCAA
    CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47650
    Clone ID Related Accession (Same CDS sequence) XM_010810691.2 , XM_010820106.2 , XM_010810691.3
    Accession Version XM_010810691.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTATCTTG ATAATGGGAG ACCGGTTTAT TTGTTCTCTA ATTGCCCAGA TTTCTTTCAA
    CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808993.1
    CDS633..3731
    Translation

    Target ORF information:

    RefSeq Version XM_010810691.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810691.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTATCTTG ATAATGGGAG ACCGGTTTAT TTGTTCTCTA ATTGCCCAGA TTTCTTTCAA
    CTGGTAAACA TCCTCCTTCC GCCAAAGGAA CTCCTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47651
    Clone ID Related Accession (Same CDS sequence) XM_010810692.2 , XM_010820109.2 , XM_010810692.3 , XM_024999840.1
    Accession Version XM_010810692.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808994.1
    CDS358..3363
    Translation

    Target ORF information:

    RefSeq Version XM_010810692.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47651
    Clone ID Related Accession (Same CDS sequence) XM_010810692.2 , XM_010820109.2 , XM_010810692.3 , XM_024999840.1
    Accession Version XM_010820109.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818411.1
    CDS358..3363
    Translation

    Target ORF information:

    RefSeq Version XM_010820109.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820109.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47651
    Clone ID Related Accession (Same CDS sequence) XM_010810692.2 , XM_010820109.2 , XM_010810692.3 , XM_024999840.1
    Accession Version XM_010810692.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808994.1
    CDS632..3637
    Translation

    Target ORF information:

    RefSeq Version XM_010810692.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810692.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47651
    Clone ID Related Accession (Same CDS sequence) XM_010810692.2 , XM_010820109.2 , XM_010810692.3 , XM_024999840.1
    Accession Version XM_024999840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TSC22 domain family protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855608.1
    CDS632..3637
    Translation

    Target ORF information:

    RefSeq Version XM_024999840.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TSC22 domain family member 1 (TSC22D1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCACCAGC CGCCCGAGTC CACGGCGGCC GCGGCCGCTG CAGACATGAG TGCTAGGAAG 
    ATGGCGCACC CGGCAATGTT CCCTCGAAGG GGCAGCGGCG GGGGCAGCGC CTCAGCTCTC
    GGTGCAGCAG GTACCGGCGT CGGTAGTAGT GCCCCATCTG CCGAGGATTT TCCGCCTCCG
    TCGCTGCTCC AGCCGCCGCC TCCTGCAGCA TCTTCTCTGT CGGGACCACA GCCTCCGCCT
    CCACAAAGCC TGAACCTCCT TTCGCAGGCT CAGCTGCAGG CACAGCCTCT TGCGCCAGGC
    GGAACGCAGA TGAAAAAGAA AAGTGGCTTC CAGATAACGA GCGTGACCCC GGCTCAGATC
    TCTGCTAGCA TCAGCTCGAA CAACAGCATC GCAGAGGACA CGGAGAGCTA CGACGACTTG
    GATGAGTCTC ACACGGAAGA TCTGTCGTCT TCCGAGATCC TTGATGTGTC ACTTTCCAGG
    GCTACGGACT TAGGGGAGCC TGAACGCAGC TCCTCGGAAG AGACTCTGAA TAACTTCCAG
    GAAGCCGAGA CACCTGGGGC GGTCTCTCCC AACCAGCCCC ACCTTCCTCA GCCTCATTTG
    CCTCACCTTC CACAACAGAA CGTTGTGATC AATGGGAATG CTCATCCACA CCCCCTCCAT
    CACCACCATC CCATTCATGG CCACCACCTG CACCACGGGC ACCACCATCC ATCCCATGCC
    GGGGTGGCCA GTACATCCAT CCCTGGAGGG CCGCCCTCAA GCCCAGTGTC CAGAAAACTC
    TCGGCCGCGG GAAGCTCTGA CGGTGTTATG CCAGTTGCAC CAACTTCTGC TGTATCATTG
    AGTGGCTCGC CGGCATCTGT CATGACTAGT GTCCGTGCTC CGAGTACTAC CGGCAGCCTA
    GGTATAAATT CTGTTACGGG CACCAATACG ATGAATAACG TTAACGTCAC TGCTGTGGGT
    AGTTTTAATC CTCCTGTGAC CAGCAGCATG CTTGGTAACG CTAATATAAG TGTGAGCAGT
    ATCCCCAGTG CTGCTAGTGT GAGTGTCGGG CCTGCAGTGA GCAGCGGGGT TAATGTGAAT
    ATCTTGAGTG GCATGGGCAA TGGTACGATT GCTTCCTCCG CGGCCTTTAA CAGCGCTGCC
    AATGCAGCAG CGGGCATGAC AGTGGGGTCC GTTTCGAGTC AGCAGCAACA GCCAACCGTT
    AACACGTCCA GGTTCAGAGT CGTGAAGTTG GATTCTACTT CTGAGCCTTT CAAAAAAGGT
    CGATGGACTT GCACTGAGTT CTATGAAAAA GAAAACGCCG CCGTACCCGC CACCGAAGGG
    GTGGTCGTAA ATAAGGTGGT GGAAAGTGTA AGACAAAACC CGACCGAAGC GACTTCTGAG
    AGGGAGAGCA CGAGTGGGAG CTCTGTGAGC AGCAGCGTCA GCACACTGAG TCACTACACG
    GAGAGTGTGG GCAGCGGAGA GATGGGCACC CTGGCAGCCC CGCCGGTGCC CCCGACCCTT
    CCAGGGGTGG CCCTTCCGCA GATGGACTTC AGTAGCGCCG CTCCACAGGG CATTTCAGCA
    GTTAGCATGC CTCAGAGTAT TTCTCAGTCG CAGATCTCGC AGGTACAGTT ACCGTCTCAA
    GAACTGGGCT ATCAGCCGAA GCCAGGTCTT CAACCAGTAC CTCTGCAAGC CGGTATTCAG
    CCGTCACCTG TTGGCGTGGT GGGCGTCACT TCGGCTTTAG GTCAGCAGCC TTCCGTCGCC
    AGCCTGGCTC AACCCCAACT GCCGTATTCC CAGGCGGCTC CCCCAGTGCA AGCTCCCCTG
    CCAGGGGCGC CACCCCAACA GGTACAATAT AGCCAGCCGG CGCCAGCTGT GGGCCCTCCG
    ATGGCCCCAA GCCATGGTAC ATCAGTGGCT CCGAACCCAG CCTCCGAGTA TGTTCAGCCC
    TCACCGCTTC TCCAAACAGC GGTATCCTCT GGACAGCCCA CTTCTGCAGG GGTGGCTGTT
    GGAGCCACGG TGATTCCTAT GGCTCAGCCA CAGAGCATCC AGCTCCCAGT GCAGCCCGCG
    GCAGTGCAAG CACAACCTGC GGGGGCAGCT GGCCAACCTG TTGGCAAGGC TCACACGACA
    GTAGCTGCTG TACCTCCTGG CAGTCAAATC GCAACTATTG GTCAACAGAC AAGCCTACCA
    TCGGCACTGC AGCAGCCCTC CACCCAAGTC ACACCTTCAG TTATCCAGCA AGGTGCTCCT
    CCGTCTTCAC AGATAGTGCC ACCTGCTCCA GCTGCGATCC TTCATCAGGG AGTTCAGCCC
    AGCGCTTCAG GCCTTCCTCA ACAACTGGTC ATTGCACCTC AGAGTACCCT ATTACCTGTG
    CCTCCCCAGC CACAGGGGGT CGAGTCGGTA GCTCCAGGAG TGGTTTCGAA GCAGTTGCCT
    GCAGTTAGTC CTTTGCCCTC TGCTAGTAGT ATTTCTGTTA CAAATCAGGT TAGTTCAGCT
    GGTCCTTCTG GACTGCCTTC TGCCCCGACA AACTTGGTCC CGTCACAGAA TATAGCACAA
    GCCCCTGTCA CTCAGAATGG TAATTTGGTT CAAAGTGTCA GTCAGCCTCC CTTGCTAGCA
    TCTAATATAA ATTTGCCTTT GGCACAACAG CTACCGCTCA GTTCTGTTCA GTTCTCCGCA
    CAATCATTAG CTCAGGCAAT TGGAAGCCAA ATCGAAGATG CCAGGCGCCC AGCGGAACCC
    TCCTTAGTTG GCTTACCTCA GACCATCAGT GGTGACAGTG GGGGAGTGTC AGCAGTTTCA
    GATGGCAGTA GCAGCAGCCT TGCAGCCTCT GCTTCTCTTT TCCCGTTGAA GGTGCTACCG
    CTGACGACAC CCCTGGTGGA TGGCGAGGAC GAGAGTGCTT CTCTCCTACC AGAGGTGCAA
    GGAGTGATCC TAGAACCACA GATACAGCCA AGACCACGGA GAGCTTTTGA CGTCAGGGGT
    CCACTTTCTC CACTGAACCT TTGGAGGCAG AATATCCAGC TTCTGGAGAG AGTTGGGAAA
    GGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.