LOC508459 cDNA ORF clone, Bos taurus(cattle)

The following LOC508459 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC508459 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236495 XM_024994938.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa236482 XM_010806933.3
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236483 XM_024994929.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236484 XM_024994930.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236485 XM_010806936.3
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236486 XM_024994931.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236487 XM_024994932.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236488 XM_024994933.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236489 XM_024994934.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236490 XM_010806939.3
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236491 XM_010806937.3
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236492 XM_024994935.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236493 XM_024994936.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa236494 XM_024994937.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa236479 XM_024994928.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236480 XM_015472057.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236481 XM_015472058.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa47159 XM_010798267.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47159 XM_010806935.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47162 XM_010806938.3
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47162 XM_010798270.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47166 XM_010798265.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47166 XM_010806933.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47168 XM_010806936.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47168 XM_010798268.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47169 XM_010798269.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47169 XM_010806937.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47170 XM_010806939.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa47170 XM_010798271.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa47171 XM_010806940.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa47171 XM_010798272.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa47172 XM_010806941.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa47172 XM_010798273.2
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa90255 XM_015472057.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90255 XM_015464106.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90256 XM_015472058.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90256 XM_015464107.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90257 XM_015464108.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90257 XM_015472059.1
Latest version!
Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa47162 XM_010806938.2 Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236495
    Clone ID Related Accession (Same CDS sequence) XM_024994938.1
    Accession Version XM_024994938.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAGAATTTCC AGGGACCAGG AGAGACATGT
    GAAGATGTGG ACGAATGTTC CAGGAACTCA ACTCTTTGTG GTCCCAGTTC TGTCTGCACC
    AACATCCCAG GAAAATACAG CTGCTCCTGC TTGCCGGGGT TCTTTTCACC TGGTGTGTGG
    AGCCCTGAAA AACCTGAGGC TTTCAAGTGT GCAGATATTG ACGAGTGCCT TCAGGATCCC
    TCACGATGCG GCCCAGATTC TGTCTGCACC AACACCCTGG GCTCCTACAG CTGTGGCTGC
    GTTGTAGGCT TTCATCCCAA TCCAGAAGGC TCCTGGAAAC ATGGCAACTT CAGCTGCCAA
    AGGATTCCCT TCAAGTGTAA AGATGATGTG ATACCCAGCA ACATGCAGGT CCAGTTATGC
    CATGCGGGAG CAGCGGTGGA GCCTAAATAT GTTTCCTTTT GTGCGCTGAT GAATGCCACC
    CTCAGCGTTC TGGACAATGT CTGTGAAAAC AAAACCACTG TCGTCTCTCT GAAGAGTACG
    GCTAAGAGAC TTGACTCTGT GATTGAAAAA ACATCCAAGT GGTCCAACTT CACCAAAGAA
    GAGACCTCGA CGCTGGCCAC AGTCTTGCTG GAGAGCGTGG AAAGCACCAC GTTGGCAGCT
    TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG ACTATTCAGA CGAAACACTT AGATATCGAG
    AGCAAAGTGA TTGACAAAGA ATGCACTAAA GAGAATGAGA CATTTAAGTT GAAAGCAAAA
    GGGGATGAAA TGAAGATTTG GTGTTCCACA ATCAAAGAAT CTGAATCCAC AGGGATCAAC
    GGGGTGGCTT TCGTTTCCTT TTCGGGTATG GAGTCTATTT TGGATGAGCG CTTCTTGAAA
    GATCTTCAGA TCCCCTGGGC CAGCTCCAAG AAGAAGCTGA AGATGAATTC TCGAGTTGTT
    GGGGGCATCA TAACAGGAGG GAAGAAAGAC GGGTTCTCTA AACCAGTAAT CTATACTCTG
    GAAAACATTA AGCCAAAGCA GGATTTTGAG AGTGCTATCT GCGTTTCCTG GAAACCGGAC
    GTGGAGGGAG GCAGATGGAC ACCATCAGGC TGTGTGCTCC TTGAAGCTTC CAAGACACAT
    ACTGTCTGCG GCTGTAACCA AATGGTGAAC TTGGCTGTCA TCATGGCTTC TGGGGAGGTC
    ACGATGGAGT TCACCCTGTA CCTCATTAGT CACGTGGGCA CGATTATCTC ACTGGTGTGT
    CTTGCCATGG CCATCATCAC CTTCCTTCTG TGCCGCACCC TCCGAAACCA GAACACCTAC
    ATCCACCTGC ATCTCTGCAT ATGCCTCTTC TTGGCCAAGA TGCTGTTTCT CACTGGTGTG
    GACAAGACCG ACAACCAGAT GGGCTGCGCC CTCATCGCAG GCTTTCTGCA CTACCTTTTC
    CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG GCTGTGATGC TCTTCCTCAT GGTCAGGAAC
    CTGAAAGTGG TGAATTACTT CAGTTCTCGA AACATCAAGA TGCTGTATCT CTGCGCCTTT
    GGCTATGGGC TCCCGGGGCT GGTGGTGGCT GTTTCTGCTG GCTTACAACC ACAGGGTTAT
    GGGATGTATA ATCGCTGCTG GCTGAATACA GAAACGGGGT TCATCTGGAG CTTCCTGGGG
    CCAGTGTGCA CAATTATAGT GGTCAATTCC ATCCTCCTGA CTTGGACACT ATGTATCCTG
    AGGCAGAAGC TTTCTAGTGT CAATGCAGAA GTCTCCACAC TCAAAGATAC CAGGTTGCTG
    ACCTTCAAAG CCTTTGCCCA GATCTTCATC TTGGGGTGCT CCTGGGTGTT AGGCATTTTC
    CAAATTGGAC CCATGGCCAA TATCATGGCT TATCTGTTCA CCATCATCAA CAGCTTGCAG
    GGGGCCTTCA TCTTCCTCAT TCATTGTGTG CTCAGCCGCC AGGTACGGGA AGAATACAGA
    AGATGCACGA CCAGGAAGAC CAAACCCAGC TCTGAGACCC AGACCTCAGG GATCTTACTG
    TCATCTGTTC CCTCCACTTC CAAAACGGGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850706.1
    CDS106..2838
    Translation

    Target ORF information:

    RefSeq Version XM_024994938.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAGAATTTCC AGGGACCAGG AGAGACATGT
    GAAGATGTGG ACGAATGTTC CAGGAACTCA ACTCTTTGTG GTCCCAGTTC TGTCTGCACC
    AACATCCCAG GAAAATACAG CTGCTCCTGC TTGCCGGGGT TCTTTTCACC TGGTGTGTGG
    AGCCCTGAAA AACCTGAGGC TTTCAAGTGT GCAGATATTG ACGAGTGCCT TCAGGATCCC
    TCACGATGCG GCCCAGATTC TGTCTGCACC AACACCCTGG GCTCCTACAG CTGTGGCTGC
    GTTGTAGGCT TTCATCCCAA TCCAGAAGGC TCCTGGAAAC ATGGCAACTT CAGCTGCCAA
    AGGATTCCCT TCAAGTGTAA AGATGATGTG ATACCCAGCA ACATGCAGGT CCAGTTATGC
    CATGCGGGAG CAGCGGTGGA GCCTAAATAT GTTTCCTTTT GTGCGCTGAT GAATGCCACC
    CTCAGCGTTC TGGACAATGT CTGTGAAAAC AAAACCACTG TCGTCTCTCT GAAGAGTACG
    GCTAAGAGAC TTGACTCTGT GATTGAAAAA ACATCCAAGT GGTCCAACTT CACCAAAGAA
    GAGACCTCGA CGCTGGCCAC AGTCTTGCTG GAGAGCGTGG AAAGCACCAC GTTGGCAGCT
    TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG ACTATTCAGA CGAAACACTT AGATATCGAG
    AGCAAAGTGA TTGACAAAGA ATGCACTAAA GAGAATGAGA CATTTAAGTT GAAAGCAAAA
    GGGGATGAAA TGAAGATTTG GTGTTCCACA ATCAAAGAAT CTGAATCCAC AGGGATCAAC
    GGGGTGGCTT TCGTTTCCTT TTCGGGTATG GAGTCTATTT TGGATGAGCG CTTCTTGAAA
    GATCTTCAGA TCCCCTGGGC CAGCTCCAAG AAGAAGCTGA AGATGAATTC TCGAGTTGTT
    GGGGGCATCA TAACAGGAGG GAAGAAAGAC GGGTTCTCTA AACCAGTAAT CTATACTCTG
    GAAAACATTA AGCCAAAGCA GGATTTTGAG AGTGCTATCT GCGTTTCCTG GAAACCGGAC
    GTGGAGGGAG GCAGATGGAC ACCATCAGGC TGTGTGCTCC TTGAAGCTTC CAAGACACAT
    ACTGTCTGCG GCTGTAACCA AATGGTGAAC TTGGCTGTCA TCATGGCTTC TGGGGAGGTC
    ACGATGGAGT TCACCCTGTA CCTCATTAGT CACGTGGGCA CGATTATCTC ACTGGTGTGT
    CTTGCCATGG CCATCATCAC CTTCCTTCTG TGCCGCACCC TCCGAAACCA GAACACCTAC
    ATCCACCTGC ATCTCTGCAT ATGCCTCTTC TTGGCCAAGA TGCTGTTTCT CACTGGTGTG
    GACAAGACCG ACAACCAGAT GGGCTGCGCC CTCATCGCAG GCTTTCTGCA CTACCTTTTC
    CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG GCTGTGATGC TCTTCCTCAT GGTCAGGAAC
    CTGAAAGTGG TGAATTACTT CAGTTCTCGA AACATCAAGA TGCTGTATCT CTGCGCCTTT
    GGCTATGGGC TCCCGGGGCT GGTGGTGGCT GTTTCTGCTG GCTTACAACC ACAGGGTTAT
    GGGATGTATA ATCGCTGCTG GCTGAATACA GAAACGGGGT TCATCTGGAG CTTCCTGGGG
    CCAGTGTGCA CAATTATAGT GGTCAATTCC ATCCTCCTGA CTTGGACACT ATGTATCCTG
    AGGCAGAAGC TTTCTAGTGT CAATGCAGAA GTCTCCACAC TCAAAGATAC CAGGTTGCTG
    ACCTTCAAAG CCTTTGCCCA GATCTTCATC TTGGGGTGCT CCTGGGTGTT AGGCATTTTC
    CAAATTGGAC CCATGGCCAA TATCATGGCT TATCTGTTCA CCATCATCAA CAGCTTGCAG
    GGGGCCTTCA TCTTCCTCAT TCATTGTGTG CTCAGCCGCC AGGTACGGGA AGAATACAGA
    AGATGCACGA CCAGGAAGAC CAAACCCAGC TCTGAGACCC AGACCTCAGG GATCTTACTG
    TCATCTGTTC CCTCCACTTC CAAAACGGGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236482
    Clone ID Related Accession (Same CDS sequence) XM_010806933.3
    Accession Version XM_010806933.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGACC ATTCGACTTG CCACAACAGT CTTGGAAACT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGGGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGACTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GATGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGACGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGAGTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GACGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    ATAGATGAAT GTTCTCAAAC ACCCCCAACT TGTGGTCCTA ACTCAAGCTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCTT CTCCCACTGG AAATAATTGG
    ATCCCTGGAA AGTCAGGTCA TTTTACCTGC ACAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCCAGAAG TGGAAAGAAG AATTTCCAGG GACCAGGAGA GACATGTGAA
    GATGTGGACG AATGTTCCAG GAACTCAACT CTTTGTGGTC CCAGTTCTGT CTGCACCAAC
    ATCCCAGGAA AATACAGCTG CTCCTGCTTG CCGGGGTTCT TTTCACCTGG TGTGTGGAGC
    CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA GATATTGACG AGTGCCTTCA GGATCCCTCA
    CGATGCGGCC CAGATTCTGT CTGCACCAAC ACCCTGGGCT CCTACAGCTG TGGCTGCGTT
    GTAGGCTTTC ATCCCAATCC AGAAGGCTCC TGGAAACATG GCAACTTCAG CTGCCAAAGG
    ATTCCCTTCA AGTGTAAAGA TGATGTGATA CCCAGCAACA TGCAGGTCCA GTTATGCCAT
    GCGGGAGCAG CGGTGGAGCC TAAATATGTT TCCTTTTGTG CGCTGATGAA TGCCACCCTC
    AGCGTTCTGG ACAATGTCTG TGAAAACAAA ACCACTGTCG TCTCTCTGAA GAGTACGGCT
    AAGAGACTTG ACTCTGTGAT TGAAAAAACA TCCAAGTGGT CCAACTTCAC CAAAGAAGAG
    ACCTCGACGC TGGCCACAGT CTTGCTGGAG AGCGTGGAAA GCACCACGTT GGCAGCTTTT
    CTGAAACCCT CAGCGAATGT CAGTCAGACT ATTCAGACGA AACACTTAGA TATCGAGAGC
    AAAGTGATTG ACAAAGAATG CACTAAAGAG AATGAGACAT TTAAGTTGAA AGCAAAAGGG
    GATGAAATGA AGATTTGGTG TTCCACAATC AAAGAATCTG AATCCACAGG GATCAACGGG
    GTGGCTTTCG TTTCCTTTTC GGGTATGGAG TCTATTTTGG ATGAGCGCTT CTTGAAAGAT
    CTTCAGATCC CCTGGGCCAG CTCCAAGAAG AAGCTGAAGA TGAATTCTCG AGTTGTTGGG
    GGCATCATAA CAGGAGGGAA GAAAGACGGG TTCTCTAAAC CAGTAATCTA TACTCTGGAA
    AACATTAAGC CAAAGCAGGA TTTTGAGAGT GCTATCTGCG TTTCCTGGAA ACCGGACGTG
    GAGGGAGGCA GATGGACACC ATCAGGCTGT GTGCTCCTTG AAGCTTCCAA GACACATACT
    GTCTGCGGCT GTAACCAAAT GGTGAACTTG GCTGTCATCA TGGCTTCTGG GGAGGTCACG
    ATGGAGTTCA CCCTGTACCT CATTAGTCAC GTGGGCACGA TTATCTCACT GGTGTGTCTT
    GCCATGGCCA TCATCACCTT CCTTCTGTGC CGCACCCTCC GAAACCAGAA CACCTACATC
    CACCTGCATC TCTGCATATG CCTCTTCTTG GCCAAGATGC TGTTTCTCAC TGGTGTGGAC
    AAGACCGACA ACCAGATGGG CTGCGCCCTC ATCGCAGGCT TTCTGCACTA CCTTTTCCTT
    GCGTGCTTCT TCTGGATGCT GGTGGAGGCT GTGATGCTCT TCCTCATGGT CAGGAACCTG
    AAAGTGGTGA ATTACTTCAG TTCTCGAAAC ATCAAGATGC TGTATCTCTG CGCCTTTGGC
    TATGGGCTCC CGGGGCTGGT GGTGGCTGTT TCTGCTGGCT TACAACCACA GGGTTATGGG
    ATGTATAATC GCTGCTGGCT GAATACAGAA ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA
    GTGTGCACAA TTATAGTGGT CAATTCCATC CTCCTGACTT GGACACTATG TATCCTGAGG
    CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC TCCACACTCA AAGATACCAG GTTGCTGACC
    TTCAAAGCCT TTGCCCAGAT CTTCATCTTG GGGTGCTCCT GGGTGTTAGG CATTTTCCAA
    ATTGGACCCA TGGCCAATAT CATGGCTTAT CTGTTCACCA TCATCAACAG CTTGCAGGGG
    GCCTTCATCT TCCTCATTCA TTGTGTGCTC AGCCGCCAGG TACGGGAAGA ATACAGAAGA
    TGCACGACCA GGAAGACCAA ACCCAGCTCT GAGACCCAGA CCTCAGGGAT CTTACTGTCA
    TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805235.2
    CDS102..4571
    Translation

    Target ORF information:

    RefSeq Version XM_010806933.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806933.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGACC ATTCGACTTG CCACAACAGT CTTGGAAACT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGGGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGACTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GATGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGACGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGAGTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GACGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    ATAGATGAAT GTTCTCAAAC ACCCCCAACT TGTGGTCCTA ACTCAAGCTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCTT CTCCCACTGG AAATAATTGG
    ATCCCTGGAA AGTCAGGTCA TTTTACCTGC ACAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCCAGAAG TGGAAAGAAG AATTTCCAGG GACCAGGAGA GACATGTGAA
    GATGTGGACG AATGTTCCAG GAACTCAACT CTTTGTGGTC CCAGTTCTGT CTGCACCAAC
    ATCCCAGGAA AATACAGCTG CTCCTGCTTG CCGGGGTTCT TTTCACCTGG TGTGTGGAGC
    CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA GATATTGACG AGTGCCTTCA GGATCCCTCA
    CGATGCGGCC CAGATTCTGT CTGCACCAAC ACCCTGGGCT CCTACAGCTG TGGCTGCGTT
    GTAGGCTTTC ATCCCAATCC AGAAGGCTCC TGGAAACATG GCAACTTCAG CTGCCAAAGG
    ATTCCCTTCA AGTGTAAAGA TGATGTGATA CCCAGCAACA TGCAGGTCCA GTTATGCCAT
    GCGGGAGCAG CGGTGGAGCC TAAATATGTT TCCTTTTGTG CGCTGATGAA TGCCACCCTC
    AGCGTTCTGG ACAATGTCTG TGAAAACAAA ACCACTGTCG TCTCTCTGAA GAGTACGGCT
    AAGAGACTTG ACTCTGTGAT TGAAAAAACA TCCAAGTGGT CCAACTTCAC CAAAGAAGAG
    ACCTCGACGC TGGCCACAGT CTTGCTGGAG AGCGTGGAAA GCACCACGTT GGCAGCTTTT
    CTGAAACCCT CAGCGAATGT CAGTCAGACT ATTCAGACGA AACACTTAGA TATCGAGAGC
    AAAGTGATTG ACAAAGAATG CACTAAAGAG AATGAGACAT TTAAGTTGAA AGCAAAAGGG
    GATGAAATGA AGATTTGGTG TTCCACAATC AAAGAATCTG AATCCACAGG GATCAACGGG
    GTGGCTTTCG TTTCCTTTTC GGGTATGGAG TCTATTTTGG ATGAGCGCTT CTTGAAAGAT
    CTTCAGATCC CCTGGGCCAG CTCCAAGAAG AAGCTGAAGA TGAATTCTCG AGTTGTTGGG
    GGCATCATAA CAGGAGGGAA GAAAGACGGG TTCTCTAAAC CAGTAATCTA TACTCTGGAA
    AACATTAAGC CAAAGCAGGA TTTTGAGAGT GCTATCTGCG TTTCCTGGAA ACCGGACGTG
    GAGGGAGGCA GATGGACACC ATCAGGCTGT GTGCTCCTTG AAGCTTCCAA GACACATACT
    GTCTGCGGCT GTAACCAAAT GGTGAACTTG GCTGTCATCA TGGCTTCTGG GGAGGTCACG
    ATGGAGTTCA CCCTGTACCT CATTAGTCAC GTGGGCACGA TTATCTCACT GGTGTGTCTT
    GCCATGGCCA TCATCACCTT CCTTCTGTGC CGCACCCTCC GAAACCAGAA CACCTACATC
    CACCTGCATC TCTGCATATG CCTCTTCTTG GCCAAGATGC TGTTTCTCAC TGGTGTGGAC
    AAGACCGACA ACCAGATGGG CTGCGCCCTC ATCGCAGGCT TTCTGCACTA CCTTTTCCTT
    GCGTGCTTCT TCTGGATGCT GGTGGAGGCT GTGATGCTCT TCCTCATGGT CAGGAACCTG
    AAAGTGGTGA ATTACTTCAG TTCTCGAAAC ATCAAGATGC TGTATCTCTG CGCCTTTGGC
    TATGGGCTCC CGGGGCTGGT GGTGGCTGTT TCTGCTGGCT TACAACCACA GGGTTATGGG
    ATGTATAATC GCTGCTGGCT GAATACAGAA ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA
    GTGTGCACAA TTATAGTGGT CAATTCCATC CTCCTGACTT GGACACTATG TATCCTGAGG
    CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC TCCACACTCA AAGATACCAG GTTGCTGACC
    TTCAAAGCCT TTGCCCAGAT CTTCATCTTG GGGTGCTCCT GGGTGTTAGG CATTTTCCAA
    ATTGGACCCA TGGCCAATAT CATGGCTTAT CTGTTCACCA TCATCAACAG CTTGCAGGGG
    GCCTTCATCT TCCTCATTCA TTGTGTGCTC AGCCGCCAGG TACGGGAAGA ATACAGAAGA
    TGCACGACCA GGAAGACCAA ACCCAGCTCT GAGACCCAGA CCTCAGGGAT CTTACTGTCA
    TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236483
    Clone ID Related Accession (Same CDS sequence) XM_024994929.1
    Accession Version XM_024994929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGACCATTC GACTTGCCAC AACAGTCTTG GAAACTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCGAG AAGTGGAAGG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGACC ATTCGACTTG CCACAACAGT CTTGGAAACT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGGGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGACTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GATGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGACGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGAGTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GACGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    ATAGATGAAT GTTCTCAAAC ACCCCCAACT TGTGGTCCTA ACTCAAGCTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCTT CTCCCACTGG AAATAATTGG
    ATCCCTGGAA AGTCAGGTCA TTTTACCTGC ACAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCCAGAAG TGGAAAGAAG AATTTCCAGG GACCAGGAGA GACATGTGAA
    GATGTGGACG AATGTTCCAG GAACTCAACT CTTTGTGGTC CCAGTTCTGT CTGCACCAAC
    ATCCCAGGAA AATACAGCTG CTCCTGCTTG CCGGGGTTCT TTTCACCTGG TGTGTGGAGC
    CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA GATATTGACG AGTGCCTTCA GGATCCCTCA
    CGATGCGGCC CAGATTCTGT CTGCACCAAC ACCCTGGGCT CCTACAGCTG TGGCTGCGTT
    GTAGGCTTTC ATCCCAATCC AGAAGGCTCC TGGAAACATG GCAACTTCAG CTGCCAAAGG
    ATTCCCTTCA AGTGTAAAGA TGATGTGATA CCCAGCAACA TGCAGGTCCA GTTATGCCAT
    GCGGGAGCAG CGGTGGAGCC TAAATATGTT TCCTTTTGTG CGCTGATGAA TGCCACCCTC
    AGCGTTCTGG ACAATGTCTG TGAAAACAAA ACCACTGTCG TCTCTCTGAA GAGTACGGCT
    AAGAGACTTG ACTCTGTGAT TGAAAAAACA TCCAAGTGGT CCAACTTCAC CAAAGAAGAG
    ACCTCGACGC TGGCCACAGT CTTGCTGGAG AGCGTGGAAA GCACCACGTT GGCAGCTTTT
    CTGAAACCCT CAGCGAATGT CAGTCAGACT ATTCAGACGA AACACTTAGA TATCGAGAGC
    AAAGTGATTG ACAAAGAATG CACTAAAGAG AATGAGACAT TTAAGTTGAA AGCAAAAGGG
    GATGAAATGA AGATTTGGTG TTCCACAATC AAAGAATCTG AATCCACAGG GATCAACGGG
    GTGGCTTTCG TTTCCTTTTC GGGTATGGAG TCTATTTTGG ATGAGCGCTT CTTGAAAGAT
    CTTCAGATCC CCTGGGCCAG CTCCAAGAAG AAGCTGAAGA TGAATTCTCG AGTTGTTGGG
    GGCATCATAA CAGGAGGGAA GAAAGACGGG TTCTCTAAAC CAGTAATCTA TACTCTGGAA
    AACATTAAGC CAAAGCAGGA TTTTGAGAGT GCTATCTGCG TTTCCTGGAA ACCGGACGTG
    GAGGGAGGCA GATGGACACC ATCAGGCTGT GTGCTCCTTG AAGCTTCCAA GACACATACT
    GTCTGCGGCT GTAACCAAAT GGTGAACTTG GCTGTCATCA TGGCTTCTGG GGAGGTCACG
    ATGGAGTTCA CCCTGTACCT CATTAGTCAC GTGGGCACGA TTATCTCACT GGTGTGTCTT
    GCCATGGCCA TCATCACCTT CCTTCTGTGC CGCACCCTCC GAAACCAGAA CACCTACATC
    CACCTGCATC TCTGCATATG CCTCTTCTTG GCCAAGATGC TGTTTCTCAC TGGTGTGGAC
    AAGACCGACA ACCAGATGGG CTGCGCCCTC ATCGCAGGCT TTCTGCACTA CCTTTTCCTT
    GCGTGCTTCT TCTGGATGCT GGTGGAGGCT GTGATGCTCT TCCTCATGGT CAGGAACCTG
    AAAGTGGTGA ATTACTTCAG TTCTCGAAAC ATCAAGATGC TGTATCTCTG CGCCTTTGGC
    TATGGGCTCC CGGGGCTGGT GGTGGCTGTT TCTGCTGGCT TACAACCACA GGGTTATGGG
    ATGTATAATC GCTGCTGGCT GAATACAGAA ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA
    GTGTGCACAA TTATAGTGGT CAATTCCATC CTCCTGACTT GGACACTATG TATCCTGAGG
    CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC TCCACACTCA AAGATACCAG GTTGCTGACC
    TTCAAAGCCT TTGCCCAGAT CTTCATCTTG GGGTGCTCCT GGGTGTTAGG CATTTTCCAA
    ATTGGACCCA TGGCCAATAT CATGGCTTAT CTGTTCACCA TCATCAACAG CTTGCAGGGG
    GCCTTCATCT TCCTCATTCA TTGTGTGCTC AGCCGCCAGG TACGGGAAGA ATACAGAAGA
    TGCACGACCA GGAAGACCAA ACCCAGCTCT GAGACCCAGA CCTCAGGGAT CTTACTGTCA
    TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850697.1
    CDS102..4571
    Translation

    Target ORF information:

    RefSeq Version XM_024994929.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGACCATTC GACTTGCCAC AACAGTCTTG GAAACTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCGAG AAGTGGAAGG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGACC ATTCGACTTG CCACAACAGT CTTGGAAACT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGGGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGACTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GATGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGACGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGAGTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GACGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    ATAGATGAAT GTTCTCAAAC ACCCCCAACT TGTGGTCCTA ACTCAAGCTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCTT CTCCCACTGG AAATAATTGG
    ATCCCTGGAA AGTCAGGTCA TTTTACCTGC ACAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCCAGAAG TGGAAAGAAG AATTTCCAGG GACCAGGAGA GACATGTGAA
    GATGTGGACG AATGTTCCAG GAACTCAACT CTTTGTGGTC CCAGTTCTGT CTGCACCAAC
    ATCCCAGGAA AATACAGCTG CTCCTGCTTG CCGGGGTTCT TTTCACCTGG TGTGTGGAGC
    CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA GATATTGACG AGTGCCTTCA GGATCCCTCA
    CGATGCGGCC CAGATTCTGT CTGCACCAAC ACCCTGGGCT CCTACAGCTG TGGCTGCGTT
    GTAGGCTTTC ATCCCAATCC AGAAGGCTCC TGGAAACATG GCAACTTCAG CTGCCAAAGG
    ATTCCCTTCA AGTGTAAAGA TGATGTGATA CCCAGCAACA TGCAGGTCCA GTTATGCCAT
    GCGGGAGCAG CGGTGGAGCC TAAATATGTT TCCTTTTGTG CGCTGATGAA TGCCACCCTC
    AGCGTTCTGG ACAATGTCTG TGAAAACAAA ACCACTGTCG TCTCTCTGAA GAGTACGGCT
    AAGAGACTTG ACTCTGTGAT TGAAAAAACA TCCAAGTGGT CCAACTTCAC CAAAGAAGAG
    ACCTCGACGC TGGCCACAGT CTTGCTGGAG AGCGTGGAAA GCACCACGTT GGCAGCTTTT
    CTGAAACCCT CAGCGAATGT CAGTCAGACT ATTCAGACGA AACACTTAGA TATCGAGAGC
    AAAGTGATTG ACAAAGAATG CACTAAAGAG AATGAGACAT TTAAGTTGAA AGCAAAAGGG
    GATGAAATGA AGATTTGGTG TTCCACAATC AAAGAATCTG AATCCACAGG GATCAACGGG
    GTGGCTTTCG TTTCCTTTTC GGGTATGGAG TCTATTTTGG ATGAGCGCTT CTTGAAAGAT
    CTTCAGATCC CCTGGGCCAG CTCCAAGAAG AAGCTGAAGA TGAATTCTCG AGTTGTTGGG
    GGCATCATAA CAGGAGGGAA GAAAGACGGG TTCTCTAAAC CAGTAATCTA TACTCTGGAA
    AACATTAAGC CAAAGCAGGA TTTTGAGAGT GCTATCTGCG TTTCCTGGAA ACCGGACGTG
    GAGGGAGGCA GATGGACACC ATCAGGCTGT GTGCTCCTTG AAGCTTCCAA GACACATACT
    GTCTGCGGCT GTAACCAAAT GGTGAACTTG GCTGTCATCA TGGCTTCTGG GGAGGTCACG
    ATGGAGTTCA CCCTGTACCT CATTAGTCAC GTGGGCACGA TTATCTCACT GGTGTGTCTT
    GCCATGGCCA TCATCACCTT CCTTCTGTGC CGCACCCTCC GAAACCAGAA CACCTACATC
    CACCTGCATC TCTGCATATG CCTCTTCTTG GCCAAGATGC TGTTTCTCAC TGGTGTGGAC
    AAGACCGACA ACCAGATGGG CTGCGCCCTC ATCGCAGGCT TTCTGCACTA CCTTTTCCTT
    GCGTGCTTCT TCTGGATGCT GGTGGAGGCT GTGATGCTCT TCCTCATGGT CAGGAACCTG
    AAAGTGGTGA ATTACTTCAG TTCTCGAAAC ATCAAGATGC TGTATCTCTG CGCCTTTGGC
    TATGGGCTCC CGGGGCTGGT GGTGGCTGTT TCTGCTGGCT TACAACCACA GGGTTATGGG
    ATGTATAATC GCTGCTGGCT GAATACAGAA ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA
    GTGTGCACAA TTATAGTGGT CAATTCCATC CTCCTGACTT GGACACTATG TATCCTGAGG
    CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC TCCACACTCA AAGATACCAG GTTGCTGACC
    TTCAAAGCCT TTGCCCAGAT CTTCATCTTG GGGTGCTCCT GGGTGTTAGG CATTTTCCAA
    ATTGGACCCA TGGCCAATAT CATGGCTTAT CTGTTCACCA TCATCAACAG CTTGCAGGGG
    GCCTTCATCT TCCTCATTCA TTGTGTGCTC AGCCGCCAGG TACGGGAAGA ATACAGAAGA
    TGCACGACCA GGAAGACCAA ACCCAGCTCT GAGACCCAGA CCTCAGGGAT CTTACTGTCA
    TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236484
    Clone ID Related Accession (Same CDS sequence) XM_024994930.1
    Accession Version XM_024994930.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGACC ATTCGACTTG CCACAACAGT CTTGGAAACT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGGGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGACTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GATGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGACGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGAGTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GACGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    ATAGATGAAT GTTCTCAAAC ACCCCCAACT TGTGGTCCTA ACTCAAGCTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCTT CTCCCACTGG AAATAATTGG
    ATCCCTGGAA AGTCAGGTCA TTTTACCTGC ACAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCCAGAAG TGGAAAGAAG AATTTCCAGG GACCAGGAGA GACATGTGAA
    GATGTGGACG AATGTTCCAG GAACTCAACT CTTTGTGGTC CCAGTTCTGT CTGCACCAAC
    ATCCCAGGAA AATACAGCTG CTCCTGCTTG CCGGGGTTCT TTTCACCTGG TGTGTGGAGC
    CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA GATATTGACG AGTGCCTTCA GGATCCCTCA
    CGATGCGGCC CAGATTCTGT CTGCACCAAC ACCCTGGGCT CCTACAGCTG TGGCTGCGTT
    GTAGGCTTTC ATCCCAATCC AGAAGGCTCC TGGAAACATG GCAACTTCAG CTGCCAAAGG
    ATTCCCTTCA AGTGTAAAGA TGATGTGATA CCCAGCAACA TGCAGGTCCA GTTATGCCAT
    GCGGGAGCAG CGGTGGAGCC TAAATATGTT TCCTTTTGTG CGCTGATGAA TGCCACCCTC
    AGCGTTCTGG ACAATGTCTG TGAAAACAAA ACCACTGTCG TCTCTCTGAA GAGTACGGCT
    AAGAGACTTG ACTCTGTGAT TGAAAAAACA TCCAAGTGGT CCAACTTCAC CAAAGAAGAG
    ACCTCGACGC TGGCCACAGT CTTGCTGGAG AGCGTGGAAA GCACCACGTT GGCAGCTTTT
    CTGAAACCCT CAGCGAATGT CAGTCAGACT ATTCAGACGA AACACTTAGA TATCGAGAGC
    AAAGTGATTG ACAAAGAATG CACTAAAGAG AATGAGACAT TTAAGTTGAA AGCAAAAGGG
    GATGAAATGA AGATTTGGTG TTCCACAATC AAAGAATCTG AATCCACAGG GATCAACGGG
    GTGGCTTTCG TTTCCTTTTC GGGTATGGAG TCTATTTTGG ATGAGCGCTT CTTGAAAGAT
    CTTCAGATCC CCTGGGCCAG CTCCAAGAAG AAGCTGAAGA TGAATTCTCG AGTTGTTGGG
    GGCATCATAA CAGGAGGGAA GAAAGACGGG TTCTCTAAAC CAGTAATCTA TACTCTGGAA
    AACATTAAGC CAAAGCAGGA TTTTGAGAGT GCTATCTGCG TTTCCTGGAA ACCGGACGTG
    GAGGGAGGCA GATGGACACC ATCAGGCTGT GTGCTCCTTG AAGCTTCCAA GACACATACT
    GTCTGCGGCT GTAACCAAAT GGTGAACTTG GCTGTCATCA TGGCTTCTGG GGAGGTCACG
    ATGGAGTTCA CCCTGTACCT CATTAGTCAC GTGGGCACGA TTATCTCACT GGTGTGTCTT
    GCCATGGCCA TCATCACCTT CCTTCTGTGC CGCACCCTCC GAAACCAGAA CACCTACATC
    CACCTGCATC TCTGCATATG CCTCTTCTTG GCCAAGATGC TGTTTCTCAC TGGTGTGGAC
    AAGACCGACA ACCAGATGGG CTGCGCCCTC ATCGCAGGCT TTCTGCACTA CCTTTTCCTT
    GCGTGCTTCT TCTGGATGCT GGTGGAGGCT GTGATGCTCT TCCTCATGGT CAGGAACCTG
    AAAGTGGTGA ATTACTTCAG TTCTCGAAAC ATCAAGATGC TGTATCTCTG CGCCTTTGGC
    TATGGGCTCC CGGGGCTGGT GGTGGCTGTT TCTGCTGGCT TACAACCACA GGGTTATGGG
    ATGTATAATC GCTGCTGGCT GAATACAGAA ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA
    GTGTGCACAA TTATAGTGGT CAATTCCATC CTCCTGACTT GGACACTATG TATCCTGAGG
    CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC TCCACACTCA AAGATACCAG GTTGCTGACC
    TTCAAAGCCT TTGCCCAGAT CTTCATCTTG GGGTGCTCCT GGGTGTTAGG CATTTTCCAA
    ATTGGACCCA TGGCCAATAT CATGGCTTAT CTGTTCACCA TCATCAACAG CTTGCAGGGG
    GCCTTCATCT TCCTCATTCA TTGTGTGCTC AGCCGCCAGG TACGGGAAGA ATACAGAAGA
    TGCACGACCA GGAAGACCAA ACCCAGCTCT GAGACCCAGA CCTCAGGGAT CTTACTGTCA
    TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850698.1
    CDS102..4571
    Translation

    Target ORF information:

    RefSeq Version XM_024994930.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994930.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGACC ATTCGACTTG CCACAACAGT CTTGGAAACT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGGGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGACTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GATGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGACGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGAGTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GACGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    ATAGATGAAT GTTCTCAAAC ACCCCCAACT TGTGGTCCTA ACTCAAGCTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCTT CTCCCACTGG AAATAATTGG
    ATCCCTGGAA AGTCAGGTCA TTTTACCTGC ACAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCCAGAAG TGGAAAGAAG AATTTCCAGG GACCAGGAGA GACATGTGAA
    GATGTGGACG AATGTTCCAG GAACTCAACT CTTTGTGGTC CCAGTTCTGT CTGCACCAAC
    ATCCCAGGAA AATACAGCTG CTCCTGCTTG CCGGGGTTCT TTTCACCTGG TGTGTGGAGC
    CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA GATATTGACG AGTGCCTTCA GGATCCCTCA
    CGATGCGGCC CAGATTCTGT CTGCACCAAC ACCCTGGGCT CCTACAGCTG TGGCTGCGTT
    GTAGGCTTTC ATCCCAATCC AGAAGGCTCC TGGAAACATG GCAACTTCAG CTGCCAAAGG
    ATTCCCTTCA AGTGTAAAGA TGATGTGATA CCCAGCAACA TGCAGGTCCA GTTATGCCAT
    GCGGGAGCAG CGGTGGAGCC TAAATATGTT TCCTTTTGTG CGCTGATGAA TGCCACCCTC
    AGCGTTCTGG ACAATGTCTG TGAAAACAAA ACCACTGTCG TCTCTCTGAA GAGTACGGCT
    AAGAGACTTG ACTCTGTGAT TGAAAAAACA TCCAAGTGGT CCAACTTCAC CAAAGAAGAG
    ACCTCGACGC TGGCCACAGT CTTGCTGGAG AGCGTGGAAA GCACCACGTT GGCAGCTTTT
    CTGAAACCCT CAGCGAATGT CAGTCAGACT ATTCAGACGA AACACTTAGA TATCGAGAGC
    AAAGTGATTG ACAAAGAATG CACTAAAGAG AATGAGACAT TTAAGTTGAA AGCAAAAGGG
    GATGAAATGA AGATTTGGTG TTCCACAATC AAAGAATCTG AATCCACAGG GATCAACGGG
    GTGGCTTTCG TTTCCTTTTC GGGTATGGAG TCTATTTTGG ATGAGCGCTT CTTGAAAGAT
    CTTCAGATCC CCTGGGCCAG CTCCAAGAAG AAGCTGAAGA TGAATTCTCG AGTTGTTGGG
    GGCATCATAA CAGGAGGGAA GAAAGACGGG TTCTCTAAAC CAGTAATCTA TACTCTGGAA
    AACATTAAGC CAAAGCAGGA TTTTGAGAGT GCTATCTGCG TTTCCTGGAA ACCGGACGTG
    GAGGGAGGCA GATGGACACC ATCAGGCTGT GTGCTCCTTG AAGCTTCCAA GACACATACT
    GTCTGCGGCT GTAACCAAAT GGTGAACTTG GCTGTCATCA TGGCTTCTGG GGAGGTCACG
    ATGGAGTTCA CCCTGTACCT CATTAGTCAC GTGGGCACGA TTATCTCACT GGTGTGTCTT
    GCCATGGCCA TCATCACCTT CCTTCTGTGC CGCACCCTCC GAAACCAGAA CACCTACATC
    CACCTGCATC TCTGCATATG CCTCTTCTTG GCCAAGATGC TGTTTCTCAC TGGTGTGGAC
    AAGACCGACA ACCAGATGGG CTGCGCCCTC ATCGCAGGCT TTCTGCACTA CCTTTTCCTT
    GCGTGCTTCT TCTGGATGCT GGTGGAGGCT GTGATGCTCT TCCTCATGGT CAGGAACCTG
    AAAGTGGTGA ATTACTTCAG TTCTCGAAAC ATCAAGATGC TGTATCTCTG CGCCTTTGGC
    TATGGGCTCC CGGGGCTGGT GGTGGCTGTT TCTGCTGGCT TACAACCACA GGGTTATGGG
    ATGTATAATC GCTGCTGGCT GAATACAGAA ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA
    GTGTGCACAA TTATAGTGGT CAATTCCATC CTCCTGACTT GGACACTATG TATCCTGAGG
    CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC TCCACACTCA AAGATACCAG GTTGCTGACC
    TTCAAAGCCT TTGCCCAGAT CTTCATCTTG GGGTGCTCCT GGGTGTTAGG CATTTTCCAA
    ATTGGACCCA TGGCCAATAT CATGGCTTAT CTGTTCACCA TCATCAACAG CTTGCAGGGG
    GCCTTCATCT TCCTCATTCA TTGTGTGCTC AGCCGCCAGG TACGGGAAGA ATACAGAAGA
    TGCACGACCA GGAAGACCAA ACCCAGCTCT GAGACCCAGA CCTCAGGGAT CTTACTGTCA
    TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236485
    Clone ID Related Accession (Same CDS sequence) XM_010806936.3
    Accession Version XM_010806936.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAC GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805238.2
    CDS102..4562
    Translation

    Target ORF information:

    RefSeq Version XM_010806936.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806936.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAC GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236486
    Clone ID Related Accession (Same CDS sequence) XM_024994931.1
    Accession Version XM_024994931.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATGTGGAT
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCCACTT GTCACAACAG TCTTGGAAGC
    TACTCTTGTG TCTGCAACCC GGGTTTTCAA TCGAGAAGTG GAAGGAAAAG TTTCCAGGGG
    CCAGGAGAGA TGTGTCAAGA TGTGGACGAA TGTGCCAATC CCAGAAGTTG CCCAGAGCAT
    TCAACTTGCC ACAACAGTGT TGGAAGCTAC TCTTGTGCCT GCAACTCAGG ATATATATCC
    AGAAGTGGAA AGAAGAGTTT CCAGGGACCA GGGGAGACAT GTCAAGATAT AGACGAATGT
    TCTCAAAAGC CCCCACCTTG TGGTCCTAAC TCAAGGTGCA GAAACCTGCC AGGAAGGTAT
    GAGTGCAGCT GTTTGACTGG TTTCTCCTCT CCCACGGGAA ATAACTGGAT CTCTGGAAAG
    CCAGGTCATT TCACCTGTAC AGACATTAAT GAATGCCTCT CCCATGGGGT CTGCCCTGAG
    CATTCTGAGT GCACCAACTC TTTGGGAAGC TACCGTTGCA GCTGCAAAGT TGGATTCACC
    TCTGGAAACT CCACCTGTGA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCCACTT GTCACAACAG TCTTGGAAGC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCCAGAAGTG GAAAGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TATAGATGAA
    TGTTCTCAAA CACCCCCAAC TTGTGGTCCT AACTCAAGCT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCT TCTCCCACTG GAAATAATTG GATCCCTGGA
    AAGTCAGGTC ATTTTACCTG CACAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850699.1
    CDS101..4441
    Translation

    Target ORF information:

    RefSeq Version XM_024994931.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATGTGGAT
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCCACTT GTCACAACAG TCTTGGAAGC
    TACTCTTGTG TCTGCAACCC GGGTTTTCAA TCGAGAAGTG GAAGGAAAAG TTTCCAGGGG
    CCAGGAGAGA TGTGTCAAGA TGTGGACGAA TGTGCCAATC CCAGAAGTTG CCCAGAGCAT
    TCAACTTGCC ACAACAGTGT TGGAAGCTAC TCTTGTGCCT GCAACTCAGG ATATATATCC
    AGAAGTGGAA AGAAGAGTTT CCAGGGACCA GGGGAGACAT GTCAAGATAT AGACGAATGT
    TCTCAAAAGC CCCCACCTTG TGGTCCTAAC TCAAGGTGCA GAAACCTGCC AGGAAGGTAT
    GAGTGCAGCT GTTTGACTGG TTTCTCCTCT CCCACGGGAA ATAACTGGAT CTCTGGAAAG
    CCAGGTCATT TCACCTGTAC AGACATTAAT GAATGCCTCT CCCATGGGGT CTGCCCTGAG
    CATTCTGAGT GCACCAACTC TTTGGGAAGC TACCGTTGCA GCTGCAAAGT TGGATTCACC
    TCTGGAAACT CCACCTGTGA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCCACTT GTCACAACAG TCTTGGAAGC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCCAGAAGTG GAAAGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TATAGATGAA
    TGTTCTCAAA CACCCCCAAC TTGTGGTCCT AACTCAAGCT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCT TCTCCCACTG GAAATAATTG GATCCCTGGA
    AAGTCAGGTC ATTTTACCTG CACAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236487
    Clone ID Related Accession (Same CDS sequence) XM_024994932.1
    Accession Version XM_024994932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCCACTT GTCACAACAG TCTTGGAAGC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCCAGAAGTG GAAAGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TATAGATGAA
    TGTTCTCAAA CACCCCCAAC TTGTGGTCCT AACTCAAGCT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCT TCTCCCACTG GAAATAATTG GATCCCTGGA
    AAGTCAGGTC ATTTTACCTG CACAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850700.1
    CDS102..4442
    Translation

    Target ORF information:

    RefSeq Version XM_024994932.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCCACTT GTCACAACAG TCTTGGAAGC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCCAGAAGTG GAAAGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TATAGATGAA
    TGTTCTCAAA CACCCCCAAC TTGTGGTCCT AACTCAAGCT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCT TCTCCCACTG GAAATAATTG GATCCCTGGA
    AAGTCAGGTC ATTTTACCTG CACAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236488
    Clone ID Related Accession (Same CDS sequence) XM_024994933.1
    Accession Version XM_024994933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGACCA TTCGACTTGC
    CACAACAGTC TTGGAAACTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC GAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC AACTTGCCAC AACAGTGTTG GAAGCTACTC TTGTGCCTGC
    AACTCAGGAT ATATATCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGGGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850701.1
    CDS102..4424
    Translation

    Target ORF information:

    RefSeq Version XM_024994933.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGACCA TTCGACTTGC
    CACAACAGTC TTGGAAACTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC GAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC AACTTGCCAC AACAGTGTTG GAAGCTACTC TTGTGCCTGC
    AACTCAGGAT ATATATCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGGGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236489
    Clone ID Related Accession (Same CDS sequence) XM_024994934.1
    Accession Version XM_024994934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC AACTTGCCAC AACAGTGTTG GAAGCTACTC TTGTGCCTGC
    AACTCAGGAT ATATATCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGGGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850702.1
    CDS102..4424
    Translation

    Target ORF information:

    RefSeq Version XM_024994934.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC AACTTGCCAC AACAGTGTTG GAAGCTACTC TTGTGCCTGC
    AACTCAGGAT ATATATCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGGGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236490
    Clone ID Related Accession (Same CDS sequence) XM_010806939.3
    Accession Version XM_010806939.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCCA CTTGTCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATATAGAT GAATGTTCTC AAACACCCCC AACTTGTGGT
    CCTAACTCAA GCTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCTTCTCCCA CTGGAAATAA TTGGATCCCT GGAAAGTCAG GTCATTTTAC CTGCACAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805241.2
    CDS102..4295
    Translation

    Target ORF information:

    RefSeq Version XM_010806939.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806939.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCCA CTTGTCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATATAGAT GAATGTTCTC AAACACCCCC AACTTGTGGT
    CCTAACTCAA GCTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCTTCTCCCA CTGGAAATAA TTGGATCCCT GGAAAGTCAG GTCATTTTAC CTGCACAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236491
    Clone ID Related Accession (Same CDS sequence) XM_010806937.3
    Accession Version XM_010806937.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCCA CTTGTCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCGAGAA GTGGAAGGAA AAGTTTCCAG GGGCCAGGAG AGATGTGTCA AGATGTGGAC
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCAACTT GCCACAACAG TGTTGGAAGC
    TACTCTTGTG CCTGCAACTC AGGATATATA TCCAGAAGTG GAAAGAAGAG TTTCCAGGGA
    CCAGGGGAGA CATGTCAAGA TATAGACGAA TGTTCTCAAA AGCCCCCACC TTGTGGTCCT
    AACTCAAGGT GCAGAAACCT GCCAGGAAGG TATGAGTGCA GCTGTTTGAC TGGTTTCTCC
    TCTCCCACGG GAAATAACTG GATCTCTGGA AAGCCAGGTC ATTTCACCTG TACAGACATT
    AATGAATGCC TCTCCCATGG GGTCTGCCCT GAGCATTCTG AGTGCACCAA CTCTTTGGGA
    AGCTACCGTT GCAGCTGCAA AGTTGGATTC ACCTCTGGAA ACTCCACCTG TGAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCCA CTTGTCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATATAGAT GAATGTTCTC AAACACCCCC AACTTGTGGT
    CCTAACTCAA GCTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCTTCTCCCA CTGGAAATAA TTGGATCCCT GGAAAGTCAG GTCATTTTAC CTGCACAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805239.2
    CDS102..4295
    Translation

    Target ORF information:

    RefSeq Version XM_010806937.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCCA CTTGTCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCGAGAA GTGGAAGGAA AAGTTTCCAG GGGCCAGGAG AGATGTGTCA AGATGTGGAC
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCAACTT GCCACAACAG TGTTGGAAGC
    TACTCTTGTG CCTGCAACTC AGGATATATA TCCAGAAGTG GAAAGAAGAG TTTCCAGGGA
    CCAGGGGAGA CATGTCAAGA TATAGACGAA TGTTCTCAAA AGCCCCCACC TTGTGGTCCT
    AACTCAAGGT GCAGAAACCT GCCAGGAAGG TATGAGTGCA GCTGTTTGAC TGGTTTCTCC
    TCTCCCACGG GAAATAACTG GATCTCTGGA AAGCCAGGTC ATTTCACCTG TACAGACATT
    AATGAATGCC TCTCCCATGG GGTCTGCCCT GAGCATTCTG AGTGCACCAA CTCTTTGGGA
    AGCTACCGTT GCAGCTGCAA AGTTGGATTC ACCTCTGGAA ACTCCACCTG TGAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCCA CTTGTCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATATAGAT GAATGTTCTC AAACACCCCC AACTTGTGGT
    CCTAACTCAA GCTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCTTCTCCCA CTGGAAATAA TTGGATCCCT GGAAAGTCAG GTCATTTTAC CTGCACAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236492
    Clone ID Related Accession (Same CDS sequence) XM_024994935.1
    Accession Version XM_024994935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850703.1
    CDS102..4148
    Translation

    Target ORF information:

    RefSeq Version XM_024994935.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236493
    Clone ID Related Accession (Same CDS sequence) XM_024994936.1
    Accession Version XM_024994936.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAC GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATATA GATGAATGTT CTCAAACACC CCCAACTTGT
    GGTCCTAACT CAAGCTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCTTCTC CCACTGGAAA TAATTGGATC CCTGGAAAGT CAGGTCATTT TACCTGCACA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT
    TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT GTGGACGAAT GTTCCAGGAA CTCAACTCTT
    TGTGGTCCCA GTTCTGTCTG CACCAACATC CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG
    GGGTTCTTTT CACCTGGTGT GTGGAGCCCT GAAAAACCTG AGGCTTTCAA GTGTGCAGAT
    ATTGACGAGT GCCTTCAGGA TCCCTCACGA TGCGGCCCAG ATTCTGTCTG CACCAACACC
    CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA GGCTTTCATC CCAATCCAGA AGGCTCCTGG
    AAACATGGCA ACTTCAGCTG CCAAAGGATT CCCTTCAAGT GTAAAGATGA TGTGATACCC
    AGCAACATGC AGGTCCAGTT ATGCCATGCG GGAGCAGCGG TGGAGCCTAA ATATGTTTCC
    TTTTGTGCGC TGATGAATGC CACCCTCAGC GTTCTGGACA ATGTCTGTGA AAACAAAACC
    ACTGTCGTCT CTCTGAAGAT GGAGTTCACC CTGTACCTCA TTAGTCACGT GGGCACGATT
    ATCTCACTGG TGTGTCTTGC CATGGCCATC ATCACCTTCC TTCTGTGCCG CACCCTCCGA
    AACCAGAACA CCTACATCCA CCTGCATCTC TGCATATGCC TCTTCTTGGC CAAGATGCTG
    TTTCTCACTG GTGTGGACAA GACCGACAAC CAGATGGGCT GCGCCCTCAT CGCAGGCTTT
    CTGCACTACC TTTTCCTTGC GTGCTTCTTC TGGATGCTGG TGGAGGCTGT GATGCTCTTC
    CTCATGGTCA GGAACCTGAA AGTGGTGAAT TACTTCAGTT CTCGAAACAT CAAGATGCTG
    TATCTCTGCG CCTTTGGCTA TGGGCTCCCG GGGCTGGTGG TGGCTGTTTC TGCTGGCTTA
    CAACCACAGG GTTATGGGAT GTATAATCGC TGCTGGCTGA ATACAGAAAC GGGGTTCATC
    TGGAGCTTCC TGGGGCCAGT GTGCACAATT ATAGTGGTCA ATTCCATCCT CCTGACTTGG
    ACACTATGTA TCCTGAGGCA GAAGCTTTCT AGTGTCAATG CAGAAGTCTC CACACTCAAA
    GATACCAGGT TGCTGACCTT CAAAGCCTTT GCCCAGATCT TCATCTTGGG GTGCTCCTGG
    GTGTTAGGCA TTTTCCAAAT TGGACCCATG GCCAATATCA TGGCTTATCT GTTCACCATC
    ATCAACAGCT TGCAGGGGGC CTTCATCTTC CTCATTCATT GTGTGCTCAG CCGCCAGGTA
    CGGGAAGAAT ACAGAAGATG CACGACCAGG AAGACCAAAC CCAGCTCTGA GACCCAGACC
    TCAGGGATCT TACTGTCATC TGTTCCCTCC ACTTCCAAAA CGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850704.1
    CDS101..4048
    Translation

    Target ORF information:

    RefSeq Version XM_024994936.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994936.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAC GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATATA GATGAATGTT CTCAAACACC CCCAACTTGT
    GGTCCTAACT CAAGCTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCTTCTC CCACTGGAAA TAATTGGATC CCTGGAAAGT CAGGTCATTT TACCTGCACA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT
    TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT GTGGACGAAT GTTCCAGGAA CTCAACTCTT
    TGTGGTCCCA GTTCTGTCTG CACCAACATC CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG
    GGGTTCTTTT CACCTGGTGT GTGGAGCCCT GAAAAACCTG AGGCTTTCAA GTGTGCAGAT
    ATTGACGAGT GCCTTCAGGA TCCCTCACGA TGCGGCCCAG ATTCTGTCTG CACCAACACC
    CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA GGCTTTCATC CCAATCCAGA AGGCTCCTGG
    AAACATGGCA ACTTCAGCTG CCAAAGGATT CCCTTCAAGT GTAAAGATGA TGTGATACCC
    AGCAACATGC AGGTCCAGTT ATGCCATGCG GGAGCAGCGG TGGAGCCTAA ATATGTTTCC
    TTTTGTGCGC TGATGAATGC CACCCTCAGC GTTCTGGACA ATGTCTGTGA AAACAAAACC
    ACTGTCGTCT CTCTGAAGAT GGAGTTCACC CTGTACCTCA TTAGTCACGT GGGCACGATT
    ATCTCACTGG TGTGTCTTGC CATGGCCATC ATCACCTTCC TTCTGTGCCG CACCCTCCGA
    AACCAGAACA CCTACATCCA CCTGCATCTC TGCATATGCC TCTTCTTGGC CAAGATGCTG
    TTTCTCACTG GTGTGGACAA GACCGACAAC CAGATGGGCT GCGCCCTCAT CGCAGGCTTT
    CTGCACTACC TTTTCCTTGC GTGCTTCTTC TGGATGCTGG TGGAGGCTGT GATGCTCTTC
    CTCATGGTCA GGAACCTGAA AGTGGTGAAT TACTTCAGTT CTCGAAACAT CAAGATGCTG
    TATCTCTGCG CCTTTGGCTA TGGGCTCCCG GGGCTGGTGG TGGCTGTTTC TGCTGGCTTA
    CAACCACAGG GTTATGGGAT GTATAATCGC TGCTGGCTGA ATACAGAAAC GGGGTTCATC
    TGGAGCTTCC TGGGGCCAGT GTGCACAATT ATAGTGGTCA ATTCCATCCT CCTGACTTGG
    ACACTATGTA TCCTGAGGCA GAAGCTTTCT AGTGTCAATG CAGAAGTCTC CACACTCAAA
    GATACCAGGT TGCTGACCTT CAAAGCCTTT GCCCAGATCT TCATCTTGGG GTGCTCCTGG
    GTGTTAGGCA TTTTCCAAAT TGGACCCATG GCCAATATCA TGGCTTATCT GTTCACCATC
    ATCAACAGCT TGCAGGGGGC CTTCATCTTC CTCATTCATT GTGTGCTCAG CCGCCAGGTA
    CGGGAAGAAT ACAGAAGATG CACGACCAGG AAGACCAAAC CCAGCTCTGA GACCCAGACC
    TCAGGGATCT TACTGTCATC TGTTCCCTCC ACTTCCAAAA CGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236494
    Clone ID Related Accession (Same CDS sequence) XM_024994937.1
    Accession Version XM_024994937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCGA CTTGCCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA GAATTTCCAG
    GGACCAGGAG AGACATGTGA AGATGTGGAC GAATGTTCCA GGAACTCAAC TCTTTGTGGT
    CCCAGTTCTG TCTGCACCAA CATCCCAGGA AAATACAGCT GCTCCTGCTT GCCGGGGTTC
    TTTTCACCTG GTGTGTGGAG CCCTGAAAAA CCTGAGGCTT TCAAGTGTGC AGATATTGAC
    GAGTGCCTTC AGGATCCCTC ACGATGCGGC CCAGATTCTG TCTGCACCAA CACCCTGGGC
    TCCTACAGCT GTGGCTGCGT TGTAGGCTTT CATCCCAATC CAGAAGGCTC CTGGAAACAT
    GGCAACTTCA GCTGCCAAAG GATTCCCTTC AAGTGTAAAG ATGATGTGAT ACCCAGCAAC
    ATGCAGGTCC AGTTATGCCA TGCGGGAGCA GCGGTGGAGC CTAAATATGT TTCCTTTTGT
    GCGCTGATGA ATGCCACCCT CAGCGTTCTG GACAATGTCT GTGAAAACAA AACCACTGTC
    GTCTCTCTGA AGAGTACGGC TAAGAGACTT GACTCTGTGA TTGAAAAAAC ATCCAAGTGG
    TCCAACTTCA CCAAAGAAGA GACCTCGACG CTGGCCACAG TCTTGCTGGA GAGCGTGGAA
    AGCACCACGT TGGCAGCTTT TCTGAAACCC TCAGCGAATG TCAGTCAGAC TATTCAGACG
    AAACACTTAG ATATCGAGAG CAAAGTGATT GACAAAGAAT GCACTAAAGA GAATGAGACA
    TTTAAGTTGA AAGCAAAAGG GGATGAAATG AAGATTTGGT GTTCCACAAT CAAAGAATCT
    GAATCCACAG GGATCAACGG GGTGGCTTTC GTTTCCTTTT CGGGTATGGA GTCTATTTTG
    GATGAGCGCT TCTTGAAAGA TCTTCAGATC CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG
    ATGAATTCTC GAGTTGTTGG GGGCATCATA ACAGGAGGGA AGAAAGACGG GTTCTCTAAA
    CCAGTAATCT ATACTCTGGA AAACATTAAG CCAAAGCAGG ATTTTGAGAG TGCTATCTGC
    GTTTCCTGGA AACCGGACGT GGAGGGAGGC AGATGGACAC CATCAGGCTG TGTGCTCCTT
    GAAGCTTCCA AGACACATAC TGTCTGCGGC TGTAACCAAA TGGTGAACTT GGCTGTCATC
    ATGGCTTCTG GGGAGGTCAC GATGGAGTTC ACCCTGTACC TCATTAGTCA CGTGGGCACG
    ATTATCTCAC TGGTGTGTCT TGCCATGGCC ATCATCACCT TCCTTCTGTG CCGCACCCTC
    CGAAACCAGA ACACCTACAT CCACCTGCAT CTCTGCATAT GCCTCTTCTT GGCCAAGATG
    CTGTTTCTCA CTGGTGTGGA CAAGACCGAC AACCAGATGG GCTGCGCCCT CATCGCAGGC
    TTTCTGCACT ACCTTTTCCT TGCGTGCTTC TTCTGGATGC TGGTGGAGGC TGTGATGCTC
    TTCCTCATGG TCAGGAACCT GAAAGTGGTG AATTACTTCA GTTCTCGAAA CATCAAGATG
    CTGTATCTCT GCGCCTTTGG CTATGGGCTC CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC
    TTACAACCAC AGGGTTATGG GATGTATAAT CGCTGCTGGC TGAATACAGA AACGGGGTTC
    ATCTGGAGCT TCCTGGGGCC AGTGTGCACA ATTATAGTGG TCAATTCCAT CCTCCTGACT
    TGGACACTAT GTATCCTGAG GCAGAAGCTT TCTAGTGTCA ATGCAGAAGT CTCCACACTC
    AAAGATACCA GGTTGCTGAC CTTCAAAGCC TTTGCCCAGA TCTTCATCTT GGGGTGCTCC
    TGGGTGTTAG GCATTTTCCA AATTGGACCC ATGGCCAATA TCATGGCTTA TCTGTTCACC
    ATCATCAACA GCTTGCAGGG GGCCTTCATC TTCCTCATTC ATTGTGTGCT CAGCCGCCAG
    GTACGGGAAG AATACAGAAG ATGCACGACC AGGAAGACCA AACCCAGCTC TGAGACCCAG
    ACCTCAGGGA TCTTACTGTC ATCTGTTCCC TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850705.1
    CDS102..3992
    Translation

    Target ORF information:

    RefSeq Version XM_024994937.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCGA CTTGCCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA GAATTTCCAG
    GGACCAGGAG AGACATGTGA AGATGTGGAC GAATGTTCCA GGAACTCAAC TCTTTGTGGT
    CCCAGTTCTG TCTGCACCAA CATCCCAGGA AAATACAGCT GCTCCTGCTT GCCGGGGTTC
    TTTTCACCTG GTGTGTGGAG CCCTGAAAAA CCTGAGGCTT TCAAGTGTGC AGATATTGAC
    GAGTGCCTTC AGGATCCCTC ACGATGCGGC CCAGATTCTG TCTGCACCAA CACCCTGGGC
    TCCTACAGCT GTGGCTGCGT TGTAGGCTTT CATCCCAATC CAGAAGGCTC CTGGAAACAT
    GGCAACTTCA GCTGCCAAAG GATTCCCTTC AAGTGTAAAG ATGATGTGAT ACCCAGCAAC
    ATGCAGGTCC AGTTATGCCA TGCGGGAGCA GCGGTGGAGC CTAAATATGT TTCCTTTTGT
    GCGCTGATGA ATGCCACCCT CAGCGTTCTG GACAATGTCT GTGAAAACAA AACCACTGTC
    GTCTCTCTGA AGAGTACGGC TAAGAGACTT GACTCTGTGA TTGAAAAAAC ATCCAAGTGG
    TCCAACTTCA CCAAAGAAGA GACCTCGACG CTGGCCACAG TCTTGCTGGA GAGCGTGGAA
    AGCACCACGT TGGCAGCTTT TCTGAAACCC TCAGCGAATG TCAGTCAGAC TATTCAGACG
    AAACACTTAG ATATCGAGAG CAAAGTGATT GACAAAGAAT GCACTAAAGA GAATGAGACA
    TTTAAGTTGA AAGCAAAAGG GGATGAAATG AAGATTTGGT GTTCCACAAT CAAAGAATCT
    GAATCCACAG GGATCAACGG GGTGGCTTTC GTTTCCTTTT CGGGTATGGA GTCTATTTTG
    GATGAGCGCT TCTTGAAAGA TCTTCAGATC CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG
    ATGAATTCTC GAGTTGTTGG GGGCATCATA ACAGGAGGGA AGAAAGACGG GTTCTCTAAA
    CCAGTAATCT ATACTCTGGA AAACATTAAG CCAAAGCAGG ATTTTGAGAG TGCTATCTGC
    GTTTCCTGGA AACCGGACGT GGAGGGAGGC AGATGGACAC CATCAGGCTG TGTGCTCCTT
    GAAGCTTCCA AGACACATAC TGTCTGCGGC TGTAACCAAA TGGTGAACTT GGCTGTCATC
    ATGGCTTCTG GGGAGGTCAC GATGGAGTTC ACCCTGTACC TCATTAGTCA CGTGGGCACG
    ATTATCTCAC TGGTGTGTCT TGCCATGGCC ATCATCACCT TCCTTCTGTG CCGCACCCTC
    CGAAACCAGA ACACCTACAT CCACCTGCAT CTCTGCATAT GCCTCTTCTT GGCCAAGATG
    CTGTTTCTCA CTGGTGTGGA CAAGACCGAC AACCAGATGG GCTGCGCCCT CATCGCAGGC
    TTTCTGCACT ACCTTTTCCT TGCGTGCTTC TTCTGGATGC TGGTGGAGGC TGTGATGCTC
    TTCCTCATGG TCAGGAACCT GAAAGTGGTG AATTACTTCA GTTCTCGAAA CATCAAGATG
    CTGTATCTCT GCGCCTTTGG CTATGGGCTC CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC
    TTACAACCAC AGGGTTATGG GATGTATAAT CGCTGCTGGC TGAATACAGA AACGGGGTTC
    ATCTGGAGCT TCCTGGGGCC AGTGTGCACA ATTATAGTGG TCAATTCCAT CCTCCTGACT
    TGGACACTAT GTATCCTGAG GCAGAAGCTT TCTAGTGTCA ATGCAGAAGT CTCCACACTC
    AAAGATACCA GGTTGCTGAC CTTCAAAGCC TTTGCCCAGA TCTTCATCTT GGGGTGCTCC
    TGGGTGTTAG GCATTTTCCA AATTGGACCC ATGGCCAATA TCATGGCTTA TCTGTTCACC
    ATCATCAACA GCTTGCAGGG GGCCTTCATC TTCCTCATTC ATTGTGTGCT CAGCCGCCAG
    GTACGGGAAG AATACAGAAG ATGCACGACC AGGAAGACCA AACCCAGCTC TGAGACCCAG
    ACCTCAGGGA TCTTACTGTC ATCTGTTCCC TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236479
    Clone ID Related Accession (Same CDS sequence) XM_024994928.1
    Accession Version XM_024994928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAC GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATATA GATGAATGTT CTCAAACACC CCCAACTTGT
    GGTCCTAACT CAAGCTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCTTCTC CCACTGGAAA TAATTGGATC CCTGGAAAGT CAGGTCATTT TACCTGCACA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT
    TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT GTGGACGAAT GTTCCAGGAA CTCAACTCTT
    TGTGGTCCCA GTTCTGTCTG CACCAACATC CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG
    GGGTTCTTTT CACCTGGTGT GTGGAGCCCT GAAAAACCTG AGGCTTTCAA GTGTGCAGAT
    ATTGACGAGT GCCTTCAGGA TCCCTCACGA TGCGGCCCAG ATTCTGTCTG CACCAACACC
    CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA GGCTTTCATC CCAATCCAGA AGGCTCCTGG
    AAACATGGCA ACTTCAGCTG CCAAAGGATT CCCTTCAAGT GTAAAGATGA TGTGATACCC
    AGCAACATGC AGGTCCAGTT ATGCCATGCG GGAGCAGCGG TGGAGCCTAA ATATGTTTCC
    TTTTGTGCGC TGATGAATGC CACCCTCAGC GTTCTGGACA ATGTCTGTGA AAACAAAACC
    ACTGTCGTCT CTCTGAAGAG TACGGCTAAG AGACTTGACT CTGTGATTGA AAAAACATCC
    AAGTGGTCCA ACTTCACCAA AGAAGAGACC TCGACGCTGG CCACAGTCTT GCTGGAGAGC
    GTGGAAAGCA CCACGTTGGC AGCTTTTCTG AAACCCTCAG CGAATGTCAG TCAGACTATT
    CAGACGAAAC ACTTAGATAT CGAGAGCAAA GTGATTGACA AAGAATGCAC TAAAGAGAAT
    GAGACATTTA AGTTGAAAGC AAAAGGGGAT GAAATGAAGA TTTGGTGTTC CACAATCAAA
    GAATCTGAAT CCACAGGGAT CAACGGGGTG GCTTTCGTTT CCTTTTCGGG TATGGAGTCT
    ATTTTGGATG AGCGCTTCTT GAAAGATCTT CAGATCCCCT GGGCCAGCTC CAAGAAGAAG
    CTGAAGATGA ATTCTCGAGT TGTTGGGGGC ATCATAACAG GAGGGAAGAA AGACGGGTTC
    TCTAAACCAG TAATCTATAC TCTGGAAAAC ATTAAGCCAA AGCAGGATTT TGAGAGTGCT
    ATCTGCGTTT CCTGGAAACC GGACGTGGAG GGAGGCAGAT GGACACCATC AGGCTGTGTG
    CTCCTTGAAG CTTCCAAGAC ACATACTGTC TGCGGCTGTA ACCAAATGGT GAACTTGGCT
    GTCATCATGG CTTCTGGGGA GGTCACGATG GAGTTCACCC TGTACCTCAT TAGTCACGTG
    GGCACGATTA TCTCACTGGT GTGTCTTGCC ATGGCCATCA TCACCTTCCT TCTGTGCCGC
    ACCCTCCGAA ACCAGAACAC CTACATCCAC CTGCATCTCT GCATATGCCT CTTCTTGGCC
    AAGATGCTGT TTCTCACTGG TGTGGACAAG ACCGACAACC AGATGGGCTG CGCCCTCATC
    GCAGGCTTTC TGCACTACCT TTTCCTTGCG TGCTTCTTCT GGATGCTGGT GGAGGCTGTG
    ATGCTCTTCC TCATGGTCAG GAACCTGAAA GTGGTGAATT ACTTCAGTTC TCGAAACATC
    AAGATGCTGT ATCTCTGCGC CTTTGGCTAT GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT
    GCTGGCTTAC AACCACAGGG TTATGGGATG TATAATCGCT GCTGGCTGAA TACAGAAACG
    GGGTTCATCT GGAGCTTCCT GGGGCCAGTG TGCACAATTA TAGTGGTCAA TTCCATCCTC
    CTGACTTGGA CACTATGTAT CCTGAGGCAG AAGCTTTCTA GTGTCAATGC AGAAGTCTCC
    ACACTCAAAG ATACCAGGTT GCTGACCTTC AAAGCCTTTG CCCAGATCTT CATCTTGGGG
    TGCTCCTGGG TGTTAGGCAT TTTCCAAATT GGACCCATGG CCAATATCAT GGCTTATCTG
    TTCACCATCA TCAACAGCTT GCAGGGGGCC TTCATCTTCC TCATTCATTG TGTGCTCAGC
    CGCCAGGTAC GGGAAGAATA CAGAAGATGC ACGACCAGGA AGACCAAACC CAGCTCTGAG
    ACCCAGACCT CAGGGATCTT ACTGTCATCT GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850696.1
    CDS101..4717
    Translation

    Target ORF information:

    RefSeq Version XM_024994928.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGACCATT CGACTTGCCA CAACAGTCTT
    GGAAACTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGG GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GACTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAT
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GACGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GAGTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAC GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATATA GATGAATGTT CTCAAACACC CCCAACTTGT
    GGTCCTAACT CAAGCTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCTTCTC CCACTGGAAA TAATTGGATC CCTGGAAAGT CAGGTCATTT TACCTGCACA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT
    TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT GTGGACGAAT GTTCCAGGAA CTCAACTCTT
    TGTGGTCCCA GTTCTGTCTG CACCAACATC CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG
    GGGTTCTTTT CACCTGGTGT GTGGAGCCCT GAAAAACCTG AGGCTTTCAA GTGTGCAGAT
    ATTGACGAGT GCCTTCAGGA TCCCTCACGA TGCGGCCCAG ATTCTGTCTG CACCAACACC
    CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA GGCTTTCATC CCAATCCAGA AGGCTCCTGG
    AAACATGGCA ACTTCAGCTG CCAAAGGATT CCCTTCAAGT GTAAAGATGA TGTGATACCC
    AGCAACATGC AGGTCCAGTT ATGCCATGCG GGAGCAGCGG TGGAGCCTAA ATATGTTTCC
    TTTTGTGCGC TGATGAATGC CACCCTCAGC GTTCTGGACA ATGTCTGTGA AAACAAAACC
    ACTGTCGTCT CTCTGAAGAG TACGGCTAAG AGACTTGACT CTGTGATTGA AAAAACATCC
    AAGTGGTCCA ACTTCACCAA AGAAGAGACC TCGACGCTGG CCACAGTCTT GCTGGAGAGC
    GTGGAAAGCA CCACGTTGGC AGCTTTTCTG AAACCCTCAG CGAATGTCAG TCAGACTATT
    CAGACGAAAC ACTTAGATAT CGAGAGCAAA GTGATTGACA AAGAATGCAC TAAAGAGAAT
    GAGACATTTA AGTTGAAAGC AAAAGGGGAT GAAATGAAGA TTTGGTGTTC CACAATCAAA
    GAATCTGAAT CCACAGGGAT CAACGGGGTG GCTTTCGTTT CCTTTTCGGG TATGGAGTCT
    ATTTTGGATG AGCGCTTCTT GAAAGATCTT CAGATCCCCT GGGCCAGCTC CAAGAAGAAG
    CTGAAGATGA ATTCTCGAGT TGTTGGGGGC ATCATAACAG GAGGGAAGAA AGACGGGTTC
    TCTAAACCAG TAATCTATAC TCTGGAAAAC ATTAAGCCAA AGCAGGATTT TGAGAGTGCT
    ATCTGCGTTT CCTGGAAACC GGACGTGGAG GGAGGCAGAT GGACACCATC AGGCTGTGTG
    CTCCTTGAAG CTTCCAAGAC ACATACTGTC TGCGGCTGTA ACCAAATGGT GAACTTGGCT
    GTCATCATGG CTTCTGGGGA GGTCACGATG GAGTTCACCC TGTACCTCAT TAGTCACGTG
    GGCACGATTA TCTCACTGGT GTGTCTTGCC ATGGCCATCA TCACCTTCCT TCTGTGCCGC
    ACCCTCCGAA ACCAGAACAC CTACATCCAC CTGCATCTCT GCATATGCCT CTTCTTGGCC
    AAGATGCTGT TTCTCACTGG TGTGGACAAG ACCGACAACC AGATGGGCTG CGCCCTCATC
    GCAGGCTTTC TGCACTACCT TTTCCTTGCG TGCTTCTTCT GGATGCTGGT GGAGGCTGTG
    ATGCTCTTCC TCATGGTCAG GAACCTGAAA GTGGTGAATT ACTTCAGTTC TCGAAACATC
    AAGATGCTGT ATCTCTGCGC CTTTGGCTAT GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT
    GCTGGCTTAC AACCACAGGG TTATGGGATG TATAATCGCT GCTGGCTGAA TACAGAAACG
    GGGTTCATCT GGAGCTTCCT GGGGCCAGTG TGCACAATTA TAGTGGTCAA TTCCATCCTC
    CTGACTTGGA CACTATGTAT CCTGAGGCAG AAGCTTTCTA GTGTCAATGC AGAAGTCTCC
    ACACTCAAAG ATACCAGGTT GCTGACCTTC AAAGCCTTTG CCCAGATCTT CATCTTGGGG
    TGCTCCTGGG TGTTAGGCAT TTTCCAAATT GGACCCATGG CCAATATCAT GGCTTATCTG
    TTCACCATCA TCAACAGCTT GCAGGGGGCC TTCATCTTCC TCATTCATTG TGTGCTCAGC
    CGCCAGGTAC GGGAAGAATA CAGAAGATGC ACGACCAGGA AGACCAAACC CAGCTCTGAG
    ACCCAGACCT CAGGGATCTT ACTGTCATCT GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236480
    Clone ID Related Accession (Same CDS sequence) XM_015472057.2
    Accession Version XM_015472057.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGAACAGC CACGGTGGTC ATCGTCGAGT 
    CTTGATGGGA ATGGGTGTAC AGATACCACC ATGTGTCCAG CGTATGCAAC CTGCACCAAC
    ACTTCAAGAA GTTACTACTG CTCTTGCAAA AGAGGCTTTC TGTCCAGCAA TGGATTGAAG
    ACATTTAAGG GCCCAGGAGT GGAATGCAAA GATATAGATG AATGCTCTCA AAGTCCCACA
    CCATGTGGCC CTAACTCAAT CTGCAGAAAT CTGCCTGGGA GATATAGGTG CAGCTGTATG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAC TGGATCTCAG GAAAGCAAGG TCATTTCACC
    TGTAGAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGCCAC
    AACAGTCCTG GAAGTTACTC TTGTGTCTGC AACCCTGGTT TTCAATCCAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCTAAA AGAGATGTGT GAAGATGTGG ATGAATGTGC CAGTCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGCCTGCAAC
    CCAGGATATA ACTCCAGAAG TGGAAAGAAG ACTTTCCAGG GACCAGGGGA GACATGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGACC ATTCGACTTG CCACAACAGT
    CTTGGAAACT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGACTTTC CAGGGACCAG GGGAGACATG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GACCATTCGA CTTGCCACAA CAGTCTTGGA
    AACTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCGGGAA GTGGAAGGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCAACTT GCCACAACAG TGTTGGAAGC TACTCTTGTG CCTGCAACTC AGGATATATA
    TCCAGAAGTG GAAAGAAGAC TTTCCAGGGA CCAGGGGAGA CATGTCAAGA TATAGATGAA
    TGTTCTCAAA AGCCCCCACC TTGTGGTCCT AACTCAAGGT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCC TCTCCCACGG GAAATAACTG GATCTCTGGA
    AAGCCAGGTC ATTTCACCTG TACAGACATT AATGAATGCC TCTCCCATGG GGTCTGCCCT
    GAGCATTCTG AGTGCACCAA CTCTTTGGGA AGCTACCGTT GCAGCTGCAA AGTTGGATTC
    ACCTCTGGAA ACTCCACCTG TGAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCCA CTTGTCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCGAGAA GTGGAAGGAA AAGTTTCCAG GGGCCAGGAG AGATGTGTCA AGATGTGGAC
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCAACTT GCCACAACAG TGTTGGAAGC
    TACTCTTGTG CCTGCAACTC AGGATATATA TCCAGAAGTG GAAAGAAGAG TTTCCAGGGA
    CCAGGGGAGA CATGTCAAGA TATAGACGAA TGTTCTCAAA AGCCCCCACC TTGTGGTCCT
    AACTCAAGGT GCAGAAACCT GCCAGGAAGG TATGAGTGCA GCTGTTTGAC TGGTTTCTCC
    TCTCCCACGG GAAATAACTG GATCTCTGGA AAGCCAGGTC ATTTCACCTG TACAGACATT
    AATGAATGCC TCTCCCATGG GGTCTGCCCT GAGCATTCTG AGTGCACCAA CTCTTTGGGA
    AGCTACCGTT GCAGCTGCAA AGTTGGATTC ACCTCTGGAA ACTCCACCTG TGAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCCA CTTGTCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATATAGAT GAATGTTCTC AAACACCCCC AACTTGTGGT
    CCTAACTCAA GCTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCTTCTCCCA CTGGAAATAA TTGGATCCCT GGAAAGTCAG GTCATTTTAC CTGCACAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327543.2
    CDS16..4569
    Translation

    Target ORF information:

    RefSeq Version XM_015472057.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGAACAGC CACGGTGGTC ATCGTCGAGT 
    CTTGATGGGA ATGGGTGTAC AGATACCACC ATGTGTCCAG CGTATGCAAC CTGCACCAAC
    ACTTCAAGAA GTTACTACTG CTCTTGCAAA AGAGGCTTTC TGTCCAGCAA TGGATTGAAG
    ACATTTAAGG GCCCAGGAGT GGAATGCAAA GATATAGATG AATGCTCTCA AAGTCCCACA
    CCATGTGGCC CTAACTCAAT CTGCAGAAAT CTGCCTGGGA GATATAGGTG CAGCTGTATG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAC TGGATCTCAG GAAAGCAAGG TCATTTCACC
    TGTAGAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGCCAC
    AACAGTCCTG GAAGTTACTC TTGTGTCTGC AACCCTGGTT TTCAATCCAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCTAAA AGAGATGTGT GAAGATGTGG ATGAATGTGC CAGTCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGCCTGCAAC
    CCAGGATATA ACTCCAGAAG TGGAAAGAAG ACTTTCCAGG GACCAGGGGA GACATGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGACC ATTCGACTTG CCACAACAGT
    CTTGGAAACT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGACTTTC CAGGGACCAG GGGAGACATG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GACCATTCGA CTTGCCACAA CAGTCTTGGA
    AACTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCGGGAA GTGGAAGGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCAACTT GCCACAACAG TGTTGGAAGC TACTCTTGTG CCTGCAACTC AGGATATATA
    TCCAGAAGTG GAAAGAAGAC TTTCCAGGGA CCAGGGGAGA CATGTCAAGA TATAGATGAA
    TGTTCTCAAA AGCCCCCACC TTGTGGTCCT AACTCAAGGT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCC TCTCCCACGG GAAATAACTG GATCTCTGGA
    AAGCCAGGTC ATTTCACCTG TACAGACATT AATGAATGCC TCTCCCATGG GGTCTGCCCT
    GAGCATTCTG AGTGCACCAA CTCTTTGGGA AGCTACCGTT GCAGCTGCAA AGTTGGATTC
    ACCTCTGGAA ACTCCACCTG TGAAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCCA CTTGTCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCGAGAA GTGGAAGGAA AAGTTTCCAG GGGCCAGGAG AGATGTGTCA AGATGTGGAC
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCAACTT GCCACAACAG TGTTGGAAGC
    TACTCTTGTG CCTGCAACTC AGGATATATA TCCAGAAGTG GAAAGAAGAG TTTCCAGGGA
    CCAGGGGAGA CATGTCAAGA TATAGACGAA TGTTCTCAAA AGCCCCCACC TTGTGGTCCT
    AACTCAAGGT GCAGAAACCT GCCAGGAAGG TATGAGTGCA GCTGTTTGAC TGGTTTCTCC
    TCTCCCACGG GAAATAACTG GATCTCTGGA AAGCCAGGTC ATTTCACCTG TACAGACATT
    AATGAATGCC TCTCCCATGG GGTCTGCCCT GAGCATTCTG AGTGCACCAA CTCTTTGGGA
    AGCTACCGTT GCAGCTGCAA AGTTGGATTC ACCTCTGGAA ACTCCACCTG TGAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCCA CTTGTCACAA CAGTCTTGGA
    AGCTACTCTT GTGTCTGCAA CCCGGGTTTT CAATCCAGAA GTGGAAAGAA AAGTTTCCAG
    GGGCCAGGAG AGATGTGTCA AGATATAGAT GAATGTTCTC AAACACCCCC AACTTGTGGT
    CCTAACTCAA GCTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCTTCTCCCA CTGGAAATAA TTGGATCCCT GGAAAGTCAG GTCATTTTAC CTGCACAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236481
    Clone ID Related Accession (Same CDS sequence) XM_015472058.2
    Accession Version XM_015472058.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGGGAATG GGTGTACAGA TACCACCATG 
    TGTCCAGCGT ATGCAACCTG CACCAACACT TCAAGAAGTT ACTACTGCTC TTGCAAAAGA
    GGCTTTCTGT CCAGCAATGG ATTGAAGACA TTTAAGGGCC CAGGAGTGGA ATGCAAAGAT
    ATAGATGAAT GCTCTCAAAG TCCCACACCA TGTGGCCCTA ACTCAATCTG CAGAAATCTG
    CCTGGGAGAT ATAGGTGCAG CTGTATGACT GGTTTCTCTT CTCCCACTGG AAATAACTGG
    ATCTCAGGAA AGCAAGGTCA TTTCACCTGT AGAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGCCACAAC AGTCCTGGAA GTTACTCTTG TGTCTGCAAC
    CCTGGTTTTC AATCCAGAAG TGGAAGGAAA AGTTTCCAGG GGCTAAAAGA GATGTGTGAA
    GATGTGGATG AATGTGCCAG TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGC CTGCAACCCA GGATATAACT CCAGAAGTGG AAAGAAGACT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGACCATT CGACTTGCCA CAACAGTCTT GGAAACTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GACTTTCCAG
    GGACCAGGGG AGACATGTCA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAC
    CATTCGACTT GCCACAACAG TCTTGGAAAC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCGGGAAGTG GAAGGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TGTGGATGAA
    TGTGCCAATC CCAGAAGTTG CCCAGAGCAT TCAACTTGCC ACAACAGTGT TGGAAGCTAC
    TCTTGTGCCT GCAACTCAGG ATATATATCC AGAAGTGGAA AGAAGACTTT CCAGGGACCA
    GGGGAGACAT GTCAAGATAT AGATGAATGT TCTCAAAAGC CCCCACCTTG TGGTCCTAAC
    TCAAGGTGCA GAAACCTGCC AGGAAGGTAT GAGTGCAGCT GTTTGACTGG TTTCTCCTCT
    CCCACGGGAA ATAACTGGAT CTCTGGAAAG CCAGGTCATT TCACCTGTAC AGACATTAAT
    GAATGCCTCT CCCATGGGGT CTGCCCTGAG CATTCTGAGT GCACCAACTC TTTGGGAAGC
    TACCGTTGCA GCTGCAAAGT TGGATTCACC TCTGGAAACT CCACCTGTGA AGATGTGGAT
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCCACTT GTCACAACAG TCTTGGAAGC
    TACTCTTGTG TCTGCAACCC GGGTTTTCAA TCGAGAAGTG GAAGGAAAAG TTTCCAGGGG
    CCAGGAGAGA TGTGTCAAGA TGTGGACGAA TGTGCCAATC CCAGAAGTTG CCCAGAGCAT
    TCAACTTGCC ACAACAGTGT TGGAAGCTAC TCTTGTGCCT GCAACTCAGG ATATATATCC
    AGAAGTGGAA AGAAGAGTTT CCAGGGACCA GGGGAGACAT GTCAAGATAT AGACGAATGT
    TCTCAAAAGC CCCCACCTTG TGGTCCTAAC TCAAGGTGCA GAAACCTGCC AGGAAGGTAT
    GAGTGCAGCT GTTTGACTGG TTTCTCCTCT CCCACGGGAA ATAACTGGAT CTCTGGAAAG
    CCAGGTCATT TCACCTGTAC AGACATTAAT GAATGCCTCT CCCATGGGGT CTGCCCTGAG
    CATTCTGAGT GCACCAACTC TTTGGGAAGC TACCGTTGCA GCTGCAAAGT TGGATTCACC
    TCTGGAAACT CCACCTGTGA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCCACTT GTCACAACAG TCTTGGAAGC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCCAGAAGTG GAAAGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TATAGATGAA
    TGTTCTCAAA CACCCCCAAC TTGTGGTCCT AACTCAAGCT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCT TCTCCCACTG GAAATAATTG GATCCCTGGA
    AAGTCAGGTC ATTTTACCTG CACAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327544.2
    CDS80..4600
    Translation

    Target ORF information:

    RefSeq Version XM_015472058.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472058.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGGGAATG GGTGTACAGA TACCACCATG 
    TGTCCAGCGT ATGCAACCTG CACCAACACT TCAAGAAGTT ACTACTGCTC TTGCAAAAGA
    GGCTTTCTGT CCAGCAATGG ATTGAAGACA TTTAAGGGCC CAGGAGTGGA ATGCAAAGAT
    ATAGATGAAT GCTCTCAAAG TCCCACACCA TGTGGCCCTA ACTCAATCTG CAGAAATCTG
    CCTGGGAGAT ATAGGTGCAG CTGTATGACT GGTTTCTCTT CTCCCACTGG AAATAACTGG
    ATCTCAGGAA AGCAAGGTCA TTTCACCTGT AGAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGCCACAAC AGTCCTGGAA GTTACTCTTG TGTCTGCAAC
    CCTGGTTTTC AATCCAGAAG TGGAAGGAAA AGTTTCCAGG GGCTAAAAGA GATGTGTGAA
    GATGTGGATG AATGTGCCAG TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGC CTGCAACCCA GGATATAACT CCAGAAGTGG AAAGAAGACT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGACCATT CGACTTGCCA CAACAGTCTT GGAAACTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GACTTTCCAG
    GGACCAGGGG AGACATGTCA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAC
    CATTCGACTT GCCACAACAG TCTTGGAAAC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCGGGAAGTG GAAGGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TGTGGATGAA
    TGTGCCAATC CCAGAAGTTG CCCAGAGCAT TCAACTTGCC ACAACAGTGT TGGAAGCTAC
    TCTTGTGCCT GCAACTCAGG ATATATATCC AGAAGTGGAA AGAAGACTTT CCAGGGACCA
    GGGGAGACAT GTCAAGATAT AGATGAATGT TCTCAAAAGC CCCCACCTTG TGGTCCTAAC
    TCAAGGTGCA GAAACCTGCC AGGAAGGTAT GAGTGCAGCT GTTTGACTGG TTTCTCCTCT
    CCCACGGGAA ATAACTGGAT CTCTGGAAAG CCAGGTCATT TCACCTGTAC AGACATTAAT
    GAATGCCTCT CCCATGGGGT CTGCCCTGAG CATTCTGAGT GCACCAACTC TTTGGGAAGC
    TACCGTTGCA GCTGCAAAGT TGGATTCACC TCTGGAAACT CCACCTGTGA AGATGTGGAT
    GAATGTGCCA ATCCCAGAAG TTGCCCAGAG CATTCCACTT GTCACAACAG TCTTGGAAGC
    TACTCTTGTG TCTGCAACCC GGGTTTTCAA TCGAGAAGTG GAAGGAAAAG TTTCCAGGGG
    CCAGGAGAGA TGTGTCAAGA TGTGGACGAA TGTGCCAATC CCAGAAGTTG CCCAGAGCAT
    TCAACTTGCC ACAACAGTGT TGGAAGCTAC TCTTGTGCCT GCAACTCAGG ATATATATCC
    AGAAGTGGAA AGAAGAGTTT CCAGGGACCA GGGGAGACAT GTCAAGATAT AGACGAATGT
    TCTCAAAAGC CCCCACCTTG TGGTCCTAAC TCAAGGTGCA GAAACCTGCC AGGAAGGTAT
    GAGTGCAGCT GTTTGACTGG TTTCTCCTCT CCCACGGGAA ATAACTGGAT CTCTGGAAAG
    CCAGGTCATT TCACCTGTAC AGACATTAAT GAATGCCTCT CCCATGGGGT CTGCCCTGAG
    CATTCTGAGT GCACCAACTC TTTGGGAAGC TACCGTTGCA GCTGCAAAGT TGGATTCACC
    TCTGGAAACT CCACCTGTGA AGATGTGGAT GAATGTGCCA ATCCCAGAAG TTGCCCAGAG
    CATTCCACTT GTCACAACAG TCTTGGAAGC TACTCTTGTG TCTGCAACCC GGGTTTTCAA
    TCCAGAAGTG GAAAGAAAAG TTTCCAGGGG CCAGGAGAGA TGTGTCAAGA TATAGATGAA
    TGTTCTCAAA CACCCCCAAC TTGTGGTCCT AACTCAAGCT GCAGAAACCT GCCAGGAAGG
    TATGAGTGCA GCTGTTTGAC TGGTTTCTCT TCTCCCACTG GAAATAATTG GATCCCTGGA
    AAGTCAGGTC ATTTTACCTG CACAGATGTG GATGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCGA CTTGCCACAA CAGTCTTGGA AGCTACTCTT GTGTCTGCAA CCCGGGTTTT
    CAATCCAGAA GTGGAAAGAA GAATTTCCAG GGACCAGGAG AGACATGTGA AGATGTGGAC
    GAATGTTCCA GGAACTCAAC TCTTTGTGGT CCCAGTTCTG TCTGCACCAA CATCCCAGGA
    AAATACAGCT GCTCCTGCTT GCCGGGGTTC TTTTCACCTG GTGTGTGGAG CCCTGAAAAA
    CCTGAGGCTT TCAAGTGTGC AGATATTGAC GAGTGCCTTC AGGATCCCTC ACGATGCGGC
    CCAGATTCTG TCTGCACCAA CACCCTGGGC TCCTACAGCT GTGGCTGCGT TGTAGGCTTT
    CATCCCAATC CAGAAGGCTC CTGGAAACAT GGCAACTTCA GCTGCCAAAG GATTCCCTTC
    AAGTGTAAAG ATGATGTGAT ACCCAGCAAC ATGCAGGTCC AGTTATGCCA TGCGGGAGCA
    GCGGTGGAGC CTAAATATGT TTCCTTTTGT GCGCTGATGA ATGCCACCCT CAGCGTTCTG
    GACAATGTCT GTGAAAACAA AACCACTGTC GTCTCTCTGA AGAGTACGGC TAAGAGACTT
    GACTCTGTGA TTGAAAAAAC ATCCAAGTGG TCCAACTTCA CCAAAGAAGA GACCTCGACG
    CTGGCCACAG TCTTGCTGGA GAGCGTGGAA AGCACCACGT TGGCAGCTTT TCTGAAACCC
    TCAGCGAATG TCAGTCAGAC TATTCAGACG AAACACTTAG ATATCGAGAG CAAAGTGATT
    GACAAAGAAT GCACTAAAGA GAATGAGACA TTTAAGTTGA AAGCAAAAGG GGATGAAATG
    AAGATTTGGT GTTCCACAAT CAAAGAATCT GAATCCACAG GGATCAACGG GGTGGCTTTC
    GTTTCCTTTT CGGGTATGGA GTCTATTTTG GATGAGCGCT TCTTGAAAGA TCTTCAGATC
    CCCTGGGCCA GCTCCAAGAA GAAGCTGAAG ATGAATTCTC GAGTTGTTGG GGGCATCATA
    ACAGGAGGGA AGAAAGACGG GTTCTCTAAA CCAGTAATCT ATACTCTGGA AAACATTAAG
    CCAAAGCAGG ATTTTGAGAG TGCTATCTGC GTTTCCTGGA AACCGGACGT GGAGGGAGGC
    AGATGGACAC CATCAGGCTG TGTGCTCCTT GAAGCTTCCA AGACACATAC TGTCTGCGGC
    TGTAACCAAA TGGTGAACTT GGCTGTCATC ATGGCTTCTG GGGAGGTCAC GATGGAGTTC
    ACCCTGTACC TCATTAGTCA CGTGGGCACG ATTATCTCAC TGGTGTGTCT TGCCATGGCC
    ATCATCACCT TCCTTCTGTG CCGCACCCTC CGAAACCAGA ACACCTACAT CCACCTGCAT
    CTCTGCATAT GCCTCTTCTT GGCCAAGATG CTGTTTCTCA CTGGTGTGGA CAAGACCGAC
    AACCAGATGG GCTGCGCCCT CATCGCAGGC TTTCTGCACT ACCTTTTCCT TGCGTGCTTC
    TTCTGGATGC TGGTGGAGGC TGTGATGCTC TTCCTCATGG TCAGGAACCT GAAAGTGGTG
    AATTACTTCA GTTCTCGAAA CATCAAGATG CTGTATCTCT GCGCCTTTGG CTATGGGCTC
    CCGGGGCTGG TGGTGGCTGT TTCTGCTGGC TTACAACCAC AGGGTTATGG GATGTATAAT
    CGCTGCTGGC TGAATACAGA AACGGGGTTC ATCTGGAGCT TCCTGGGGCC AGTGTGCACA
    ATTATAGTGG TCAATTCCAT CCTCCTGACT TGGACACTAT GTATCCTGAG GCAGAAGCTT
    TCTAGTGTCA ATGCAGAAGT CTCCACACTC AAAGATACCA GGTTGCTGAC CTTCAAAGCC
    TTTGCCCAGA TCTTCATCTT GGGGTGCTCC TGGGTGTTAG GCATTTTCCA AATTGGACCC
    ATGGCCAATA TCATGGCTTA TCTGTTCACC ATCATCAACA GCTTGCAGGG GGCCTTCATC
    TTCCTCATTC ATTGTGTGCT CAGCCGCCAG GTACGGGAAG AATACAGAAG ATGCACGACC
    AGGAAGACCA AACCCAGCTC TGAGACCCAG ACCTCAGGGA TCTTACTGTC ATCTGTTCCC
    TCCACTTCCA AAACGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47159
    Clone ID Related Accession (Same CDS sequence) XM_010798267.2 , XM_010806935.2
    Accession Version XM_010798267.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796569.1
    CDS75..4250
    Translation

    Target ORF information:

    RefSeq Version XM_010798267.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798267.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47159
    Clone ID Related Accession (Same CDS sequence) XM_010798267.2 , XM_010806935.2
    Accession Version XM_010806935.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805237.1
    CDS75..4250
    Translation

    Target ORF information:

    RefSeq Version XM_010806935.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806935.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCAAC TTGCCACAAC
    AGTGTTGGAA GCTACTCTTG TGCCTGCAAC TCAGGATATA TATCCAGAAG TGGAAAGAAG
    ACTTTCCAGG GACCAGGGGA GACATGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47162
    Clone ID Related Accession (Same CDS sequence) XM_010798270.2 , XM_010806938.2 , XM_010806938.3
    Accession Version XM_010798270.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATATAG ATGAATGTTC TCAAAAGCCC CCACCTTGTG GTCCTAACTC AAGGTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCCTCTCC CACGGGAAAT
    AACTGGATCT CTGGAAAGCC AGGTCATTTC ACCTGTACAG ACATTAATGA ATGCCTCTCC
    CATGGGGTCT GCCCTGAGCA TTCTGAGTGC ACCAACTCTT TGGGAAGCTA CCGTTGCAGC
    TGCAAAGTTG GATTCACCTC TGGAAACTCC ACCTGTGAAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCCACTTGT CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC GAGAAGTGGA AGGAAAAGTT TCCAGGGGCC AGGAGAGATG
    TGTCAAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC AACTTGCCAC
    AACAGTGTTG GAAGCTACTC TTGTGCCTGC AACTCAGGAT ATATATCCAG AAGTGGAAAG
    AAGAGTTTCC AGGGACCAGG GGAGACATGT CAAGATATAG ACGAATGTTC TCAAAAGCCC
    CCACCTTGTG GTCCTAACTC AAGGTGCAGA AACCTGCCAG GAAGGTATGA GTGCAGCTGT
    TTGACTGGTT TCTCCTCTCC CACGGGAAAT AACTGGATCT CTGGAAAGCC AGGTCATTTC
    ACCTGTACAG ACATTAATGA ATGCCTCTCC CATGGGGTCT GCCCTGAGCA TTCTGAGTGC
    ACCAACTCTT TGGGAAGCTA CCGTTGCAGC TGCAAAGTTG GATTCACCTC TGGAAACTCC
    ACCTGTGAAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGT
    CACAACAGTC TTGGAAGCTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC CAGAAGTGGA
    AAGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATA TAGATGAATG TTCTCAAACA
    CCCCCAACTT GTGGTCCTAA CTCAAGCTGC AGAAACCTGC CAGGAAGGTA TGAGTGCAGC
    TGTTTGACTG GTTTCTCTTC TCCCACTGGA AATAATTGGA TCCCTGGAAA GTCAGGTCAT
    TTTACCTGCA CAGATGTGGA TGAATGTGCC AATCCCAGAA GTTGCCCAGA GCATTCGACT
    TGCCACAACA GTCTTGGAAG CTACTCTTGT GTCTGCAACC CGGGTTTTCA ATCCAGAAGT
    GGAAAGAAGA ATTTCCAGGG ACCAGGAGAG ACATGTGAAG ATGTGGACGA ATGTTCCAGG
    AACTCAACTC TTTGTGGTCC CAGTTCTGTC TGCACCAACA TCCCAGGAAA ATACAGCTGC
    TCCTGCTTGC CGGGGTTCTT TTCACCTGGT GTGTGGAGCC CTGAAAAACC TGAGGCTTTC
    AAGTGTGCAG ATATTGACGA GTGCCTTCAG GATCCCTCAC GATGCGGCCC AGATTCTGTC
    TGCACCAACA CCCTGGGCTC CTACAGCTGT GGCTGCGTTG TAGGCTTTCA TCCCAATCCA
    GAAGGCTCCT GGAAACATGG CAACTTCAGC TGCCAAAGGA TTCCCTTCAA GTGTAAAGAT
    GATGTGATAC CCAGCAACAT GCAGGTCCAG TTATGCCATG CGGGAGCAGC GGTGGAGCCT
    AAATATGTTT CCTTTTGTGC GCTGATGAAT GCCACCCTCA GCGTTCTGGA CAATGTCTGT
    GAAAACAAAA CCACTGTCGT CTCTCTGAAG AGTACGGCTA AGAGACTTGA CTCTGTGATT
    GAAAAAACAT CCAAGTGGTC CAACTTCACC AAAGAAGAGA CCTCGACGCT GGCCACAGTC
    TTGCTGGAGA GCGTGGAAAG CACCACGTTG GCAGCTTTTC TGAAACCCTC AGCGAATGTC
    AGTCAGACTA TTCAGACGAA ACACTTAGAT ATCGAGAGCA AAGTGATTGA CAAAGAATGC
    ACTAAAGAGA ATGAGACATT TAAGTTGAAA GCAAAAGGGG ATGAAATGAA GATTTGGTGT
    TCCACAATCA AAGAATCTGA ATCCACAGGG ATCAACGGGG TGGCTTTCGT TTCCTTTTCG
    GGTATGGAGT CTATTTTGGA TGAGCGCTTC TTGAAAGATC TTCAGATCCC CTGGGCCAGC
    TCCAAGAAGA AGCTGAAGAT GAATTCTCGA GTTGTTGGGG GCATCATAAC AGGAGGGAAG
    AAAGACGGGT TCTCTAAACC AGTAATCTAT ACTCTGGAAA ACATTAAGCC AAAGCAGGAT
    TTTGAGAGTG CTATCTGCGT TTCCTGGAAA CCGGACGTGG AGGGAGGCAG ATGGACACCA
    TCAGGCTGTG TGCTCCTTGA AGCTTCCAAG ACACATACTG TCTGCGGCTG TAACCAAATG
    GTGAACTTGG CTGTCATCAT GGCTTCTGGG GAGGTCACGA TGGAGTTCAC CCTGTACCTC
    ATTAGTCACG TGGGCACGAT TATCTCACTG GTGTGTCTTG CCATGGCCAT CATCACCTTC
    CTTCTGTGCC GCACCCTCCG AAACCAGAAC ACCTACATCC ACCTGCATCT CTGCATATGC
    CTCTTCTTGG CCAAGATGCT GTTTCTCACT GGTGTGGACA AGACCGACAA CCAGATGGGC
    TGCGCCCTCA TCGCAGGCTT TCTGCACTAC CTTTTCCTTG CGTGCTTCTT CTGGATGCTG
    GTGGAGGCTG TGATGCTCTT CCTCATGGTC AGGAACCTGA AAGTGGTGAA TTACTTCAGT
    TCTCGAAACA TCAAGATGCT GTATCTCTGC GCCTTTGGCT ATGGGCTCCC GGGGCTGGTG
    GTGGCTGTTT CTGCTGGCTT ACAACCACAG GGTTATGGGA TGTATAATCG CTGCTGGCTG
    AATACAGAAA CGGGGTTCAT CTGGAGCTTC CTGGGGCCAG TGTGCACAAT TATAGTGGTC
    AATTCCATCC TCCTGACTTG GACACTATGT ATCCTGAGGC AGAAGCTTTC TAGTGTCAAT
    GCAGAAGTCT CCACACTCAA AGATACCAGG TTGCTGACCT TCAAAGCCTT TGCCCAGATC
    TTCATCTTGG GGTGCTCCTG GGTGTTAGGC ATTTTCCAAA TTGGACCCAT GGCCAATATC
    ATGGCTTATC TGTTCACCAT CATCAACAGC TTGCAGGGGG CCTTCATCTT CCTCATTCAT
    TGTGTGCTCA GCCGCCAGGT ACGGGAAGAA TACAGAAGAT GCACGACCAG GAAGACCAAA
    CCCAGCTCTG AGACCCAGAC CTCAGGGATC TTACTGTCAT CTGTTCCCTC CACTTCCAAA
    ACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796572.1
    CDS75..4103
    Translation

    Target ORF information:

    RefSeq Version XM_010798270.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798270.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATATAG ATGAATGTTC TCAAAAGCCC CCACCTTGTG GTCCTAACTC AAGGTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCCTCTCC CACGGGAAAT
    AACTGGATCT CTGGAAAGCC AGGTCATTTC ACCTGTACAG ACATTAATGA ATGCCTCTCC
    CATGGGGTCT GCCCTGAGCA TTCTGAGTGC ACCAACTCTT TGGGAAGCTA CCGTTGCAGC
    TGCAAAGTTG GATTCACCTC TGGAAACTCC ACCTGTGAAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCCACTTGT CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC GAGAAGTGGA AGGAAAAGTT TCCAGGGGCC AGGAGAGATG
    TGTCAAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC AACTTGCCAC
    AACAGTGTTG GAAGCTACTC TTGTGCCTGC AACTCAGGAT ATATATCCAG AAGTGGAAAG
    AAGAGTTTCC AGGGACCAGG GGAGACATGT CAAGATATAG ACGAATGTTC TCAAAAGCCC
    CCACCTTGTG GTCCTAACTC AAGGTGCAGA AACCTGCCAG GAAGGTATGA GTGCAGCTGT
    TTGACTGGTT TCTCCTCTCC CACGGGAAAT AACTGGATCT CTGGAAAGCC AGGTCATTTC
    ACCTGTACAG ACATTAATGA ATGCCTCTCC CATGGGGTCT GCCCTGAGCA TTCTGAGTGC
    ACCAACTCTT TGGGAAGCTA CCGTTGCAGC TGCAAAGTTG GATTCACCTC TGGAAACTCC
    ACCTGTGAAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGT
    CACAACAGTC TTGGAAGCTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC CAGAAGTGGA
    AAGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATA TAGATGAATG TTCTCAAACA
    CCCCCAACTT GTGGTCCTAA CTCAAGCTGC AGAAACCTGC CAGGAAGGTA TGAGTGCAGC
    TGTTTGACTG GTTTCTCTTC TCCCACTGGA AATAATTGGA TCCCTGGAAA GTCAGGTCAT
    TTTACCTGCA CAGATGTGGA TGAATGTGCC AATCCCAGAA GTTGCCCAGA GCATTCGACT
    TGCCACAACA GTCTTGGAAG CTACTCTTGT GTCTGCAACC CGGGTTTTCA ATCCAGAAGT
    GGAAAGAAGA ATTTCCAGGG ACCAGGAGAG ACATGTGAAG ATGTGGACGA ATGTTCCAGG
    AACTCAACTC TTTGTGGTCC CAGTTCTGTC TGCACCAACA TCCCAGGAAA ATACAGCTGC
    TCCTGCTTGC CGGGGTTCTT TTCACCTGGT GTGTGGAGCC CTGAAAAACC TGAGGCTTTC
    AAGTGTGCAG ATATTGACGA GTGCCTTCAG GATCCCTCAC GATGCGGCCC AGATTCTGTC
    TGCACCAACA CCCTGGGCTC CTACAGCTGT GGCTGCGTTG TAGGCTTTCA TCCCAATCCA
    GAAGGCTCCT GGAAACATGG CAACTTCAGC TGCCAAAGGA TTCCCTTCAA GTGTAAAGAT
    GATGTGATAC CCAGCAACAT GCAGGTCCAG TTATGCCATG CGGGAGCAGC GGTGGAGCCT
    AAATATGTTT CCTTTTGTGC GCTGATGAAT GCCACCCTCA GCGTTCTGGA CAATGTCTGT
    GAAAACAAAA CCACTGTCGT CTCTCTGAAG AGTACGGCTA AGAGACTTGA CTCTGTGATT
    GAAAAAACAT CCAAGTGGTC CAACTTCACC AAAGAAGAGA CCTCGACGCT GGCCACAGTC
    TTGCTGGAGA GCGTGGAAAG CACCACGTTG GCAGCTTTTC TGAAACCCTC AGCGAATGTC
    AGTCAGACTA TTCAGACGAA ACACTTAGAT ATCGAGAGCA AAGTGATTGA CAAAGAATGC
    ACTAAAGAGA ATGAGACATT TAAGTTGAAA GCAAAAGGGG ATGAAATGAA GATTTGGTGT
    TCCACAATCA AAGAATCTGA ATCCACAGGG ATCAACGGGG TGGCTTTCGT TTCCTTTTCG
    GGTATGGAGT CTATTTTGGA TGAGCGCTTC TTGAAAGATC TTCAGATCCC CTGGGCCAGC
    TCCAAGAAGA AGCTGAAGAT GAATTCTCGA GTTGTTGGGG GCATCATAAC AGGAGGGAAG
    AAAGACGGGT TCTCTAAACC AGTAATCTAT ACTCTGGAAA ACATTAAGCC AAAGCAGGAT
    TTTGAGAGTG CTATCTGCGT TTCCTGGAAA CCGGACGTGG AGGGAGGCAG ATGGACACCA
    TCAGGCTGTG TGCTCCTTGA AGCTTCCAAG ACACATACTG TCTGCGGCTG TAACCAAATG
    GTGAACTTGG CTGTCATCAT GGCTTCTGGG GAGGTCACGA TGGAGTTCAC CCTGTACCTC
    ATTAGTCACG TGGGCACGAT TATCTCACTG GTGTGTCTTG CCATGGCCAT CATCACCTTC
    CTTCTGTGCC GCACCCTCCG AAACCAGAAC ACCTACATCC ACCTGCATCT CTGCATATGC
    CTCTTCTTGG CCAAGATGCT GTTTCTCACT GGTGTGGACA AGACCGACAA CCAGATGGGC
    TGCGCCCTCA TCGCAGGCTT TCTGCACTAC CTTTTCCTTG CGTGCTTCTT CTGGATGCTG
    GTGGAGGCTG TGATGCTCTT CCTCATGGTC AGGAACCTGA AAGTGGTGAA TTACTTCAGT
    TCTCGAAACA TCAAGATGCT GTATCTCTGC GCCTTTGGCT ATGGGCTCCC GGGGCTGGTG
    GTGGCTGTTT CTGCTGGCTT ACAACCACAG GGTTATGGGA TGTATAATCG CTGCTGGCTG
    AATACAGAAA CGGGGTTCAT CTGGAGCTTC CTGGGGCCAG TGTGCACAAT TATAGTGGTC
    AATTCCATCC TCCTGACTTG GACACTATGT ATCCTGAGGC AGAAGCTTTC TAGTGTCAAT
    GCAGAAGTCT CCACACTCAA AGATACCAGG TTGCTGACCT TCAAAGCCTT TGCCCAGATC
    TTCATCTTGG GGTGCTCCTG GGTGTTAGGC ATTTTCCAAA TTGGACCCAT GGCCAATATC
    ATGGCTTATC TGTTCACCAT CATCAACAGC TTGCAGGGGG CCTTCATCTT CCTCATTCAT
    TGTGTGCTCA GCCGCCAGGT ACGGGAAGAA TACAGAAGAT GCACGACCAG GAAGACCAAA
    CCCAGCTCTG AGACCCAGAC CTCAGGGATC TTACTGTCAT CTGTTCCCTC CACTTCCAAA
    ACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47162
    Clone ID Related Accession (Same CDS sequence) XM_010798270.2 , XM_010806938.2 , XM_010806938.3
    Accession Version XM_010806938.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATATAG ATGAATGTTC TCAAAAGCCC CCACCTTGTG GTCCTAACTC AAGGTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCCTCTCC CACGGGAAAT
    AACTGGATCT CTGGAAAGCC AGGTCATTTC ACCTGTACAG ACATTAATGA ATGCCTCTCC
    CATGGGGTCT GCCCTGAGCA TTCTGAGTGC ACCAACTCTT TGGGAAGCTA CCGTTGCAGC
    TGCAAAGTTG GATTCACCTC TGGAAACTCC ACCTGTGAAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCCACTTGT CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC GAGAAGTGGA AGGAAAAGTT TCCAGGGGCC AGGAGAGATG
    TGTCAAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC AACTTGCCAC
    AACAGTGTTG GAAGCTACTC TTGTGCCTGC AACTCAGGAT ATATATCCAG AAGTGGAAAG
    AAGAGTTTCC AGGGACCAGG GGAGACATGT CAAGATATAG ACGAATGTTC TCAAAAGCCC
    CCACCTTGTG GTCCTAACTC AAGGTGCAGA AACCTGCCAG GAAGGTATGA GTGCAGCTGT
    TTGACTGGTT TCTCCTCTCC CACGGGAAAT AACTGGATCT CTGGAAAGCC AGGTCATTTC
    ACCTGTACAG ACATTAATGA ATGCCTCTCC CATGGGGTCT GCCCTGAGCA TTCTGAGTGC
    ACCAACTCTT TGGGAAGCTA CCGTTGCAGC TGCAAAGTTG GATTCACCTC TGGAAACTCC
    ACCTGTGAAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGT
    CACAACAGTC TTGGAAGCTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC CAGAAGTGGA
    AAGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATA TAGATGAATG TTCTCAAACA
    CCCCCAACTT GTGGTCCTAA CTCAAGCTGC AGAAACCTGC CAGGAAGGTA TGAGTGCAGC
    TGTTTGACTG GTTTCTCTTC TCCCACTGGA AATAATTGGA TCCCTGGAAA GTCAGGTCAT
    TTTACCTGCA CAGATGTGGA TGAATGTGCC AATCCCAGAA GTTGCCCAGA GCATTCGACT
    TGCCACAACA GTCTTGGAAG CTACTCTTGT GTCTGCAACC CGGGTTTTCA ATCCAGAAGT
    GGAAAGAAGA ATTTCCAGGG ACCAGGAGAG ACATGTGAAG ATGTGGACGA ATGTTCCAGG
    AACTCAACTC TTTGTGGTCC CAGTTCTGTC TGCACCAACA TCCCAGGAAA ATACAGCTGC
    TCCTGCTTGC CGGGGTTCTT TTCACCTGGT GTGTGGAGCC CTGAAAAACC TGAGGCTTTC
    AAGTGTGCAG ATATTGACGA GTGCCTTCAG GATCCCTCAC GATGCGGCCC AGATTCTGTC
    TGCACCAACA CCCTGGGCTC CTACAGCTGT GGCTGCGTTG TAGGCTTTCA TCCCAATCCA
    GAAGGCTCCT GGAAACATGG CAACTTCAGC TGCCAAAGGA TTCCCTTCAA GTGTAAAGAT
    GATGTGATAC CCAGCAACAT GCAGGTCCAG TTATGCCATG CGGGAGCAGC GGTGGAGCCT
    AAATATGTTT CCTTTTGTGC GCTGATGAAT GCCACCCTCA GCGTTCTGGA CAATGTCTGT
    GAAAACAAAA CCACTGTCGT CTCTCTGAAG AGTACGGCTA AGAGACTTGA CTCTGTGATT
    GAAAAAACAT CCAAGTGGTC CAACTTCACC AAAGAAGAGA CCTCGACGCT GGCCACAGTC
    TTGCTGGAGA GCGTGGAAAG CACCACGTTG GCAGCTTTTC TGAAACCCTC AGCGAATGTC
    AGTCAGACTA TTCAGACGAA ACACTTAGAT ATCGAGAGCA AAGTGATTGA CAAAGAATGC
    ACTAAAGAGA ATGAGACATT TAAGTTGAAA GCAAAAGGGG ATGAAATGAA GATTTGGTGT
    TCCACAATCA AAGAATCTGA ATCCACAGGG ATCAACGGGG TGGCTTTCGT TTCCTTTTCG
    GGTATGGAGT CTATTTTGGA TGAGCGCTTC TTGAAAGATC TTCAGATCCC CTGGGCCAGC
    TCCAAGAAGA AGCTGAAGAT GAATTCTCGA GTTGTTGGGG GCATCATAAC AGGAGGGAAG
    AAAGACGGGT TCTCTAAACC AGTAATCTAT ACTCTGGAAA ACATTAAGCC AAAGCAGGAT
    TTTGAGAGTG CTATCTGCGT TTCCTGGAAA CCGGACGTGG AGGGAGGCAG ATGGACACCA
    TCAGGCTGTG TGCTCCTTGA AGCTTCCAAG ACACATACTG TCTGCGGCTG TAACCAAATG
    GTGAACTTGG CTGTCATCAT GGCTTCTGGG GAGGTCACGA TGGAGTTCAC CCTGTACCTC
    ATTAGTCACG TGGGCACGAT TATCTCACTG GTGTGTCTTG CCATGGCCAT CATCACCTTC
    CTTCTGTGCC GCACCCTCCG AAACCAGAAC ACCTACATCC ACCTGCATCT CTGCATATGC
    CTCTTCTTGG CCAAGATGCT GTTTCTCACT GGTGTGGACA AGACCGACAA CCAGATGGGC
    TGCGCCCTCA TCGCAGGCTT TCTGCACTAC CTTTTCCTTG CGTGCTTCTT CTGGATGCTG
    GTGGAGGCTG TGATGCTCTT CCTCATGGTC AGGAACCTGA AAGTGGTGAA TTACTTCAGT
    TCTCGAAACA TCAAGATGCT GTATCTCTGC GCCTTTGGCT ATGGGCTCCC GGGGCTGGTG
    GTGGCTGTTT CTGCTGGCTT ACAACCACAG GGTTATGGGA TGTATAATCG CTGCTGGCTG
    AATACAGAAA CGGGGTTCAT CTGGAGCTTC CTGGGGCCAG TGTGCACAAT TATAGTGGTC
    AATTCCATCC TCCTGACTTG GACACTATGT ATCCTGAGGC AGAAGCTTTC TAGTGTCAAT
    GCAGAAGTCT CCACACTCAA AGATACCAGG TTGCTGACCT TCAAAGCCTT TGCCCAGATC
    TTCATCTTGG GGTGCTCCTG GGTGTTAGGC ATTTTCCAAA TTGGACCCAT GGCCAATATC
    ATGGCTTATC TGTTCACCAT CATCAACAGC TTGCAGGGGG CCTTCATCTT CCTCATTCAT
    TGTGTGCTCA GCCGCCAGGT ACGGGAAGAA TACAGAAGAT GCACGACCAG GAAGACCAAA
    CCCAGCTCTG AGACCCAGAC CTCAGGGATC TTACTGTCAT CTGTTCCCTC CACTTCCAAA
    ACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805240.1
    CDS75..4103
    Translation

    Target ORF information:

    RefSeq Version XM_010806938.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATATAG ATGAATGTTC TCAAAAGCCC CCACCTTGTG GTCCTAACTC AAGGTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCCTCTCC CACGGGAAAT
    AACTGGATCT CTGGAAAGCC AGGTCATTTC ACCTGTACAG ACATTAATGA ATGCCTCTCC
    CATGGGGTCT GCCCTGAGCA TTCTGAGTGC ACCAACTCTT TGGGAAGCTA CCGTTGCAGC
    TGCAAAGTTG GATTCACCTC TGGAAACTCC ACCTGTGAAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCCACTTGT CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC GAGAAGTGGA AGGAAAAGTT TCCAGGGGCC AGGAGAGATG
    TGTCAAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC AACTTGCCAC
    AACAGTGTTG GAAGCTACTC TTGTGCCTGC AACTCAGGAT ATATATCCAG AAGTGGAAAG
    AAGAGTTTCC AGGGACCAGG GGAGACATGT CAAGATATAG ACGAATGTTC TCAAAAGCCC
    CCACCTTGTG GTCCTAACTC AAGGTGCAGA AACCTGCCAG GAAGGTATGA GTGCAGCTGT
    TTGACTGGTT TCTCCTCTCC CACGGGAAAT AACTGGATCT CTGGAAAGCC AGGTCATTTC
    ACCTGTACAG ACATTAATGA ATGCCTCTCC CATGGGGTCT GCCCTGAGCA TTCTGAGTGC
    ACCAACTCTT TGGGAAGCTA CCGTTGCAGC TGCAAAGTTG GATTCACCTC TGGAAACTCC
    ACCTGTGAAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGT
    CACAACAGTC TTGGAAGCTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC CAGAAGTGGA
    AAGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATA TAGATGAATG TTCTCAAACA
    CCCCCAACTT GTGGTCCTAA CTCAAGCTGC AGAAACCTGC CAGGAAGGTA TGAGTGCAGC
    TGTTTGACTG GTTTCTCTTC TCCCACTGGA AATAATTGGA TCCCTGGAAA GTCAGGTCAT
    TTTACCTGCA CAGATGTGGA TGAATGTGCC AATCCCAGAA GTTGCCCAGA GCATTCGACT
    TGCCACAACA GTCTTGGAAG CTACTCTTGT GTCTGCAACC CGGGTTTTCA ATCCAGAAGT
    GGAAAGAAGA ATTTCCAGGG ACCAGGAGAG ACATGTGAAG ATGTGGACGA ATGTTCCAGG
    AACTCAACTC TTTGTGGTCC CAGTTCTGTC TGCACCAACA TCCCAGGAAA ATACAGCTGC
    TCCTGCTTGC CGGGGTTCTT TTCACCTGGT GTGTGGAGCC CTGAAAAACC TGAGGCTTTC
    AAGTGTGCAG ATATTGACGA GTGCCTTCAG GATCCCTCAC GATGCGGCCC AGATTCTGTC
    TGCACCAACA CCCTGGGCTC CTACAGCTGT GGCTGCGTTG TAGGCTTTCA TCCCAATCCA
    GAAGGCTCCT GGAAACATGG CAACTTCAGC TGCCAAAGGA TTCCCTTCAA GTGTAAAGAT
    GATGTGATAC CCAGCAACAT GCAGGTCCAG TTATGCCATG CGGGAGCAGC GGTGGAGCCT
    AAATATGTTT CCTTTTGTGC GCTGATGAAT GCCACCCTCA GCGTTCTGGA CAATGTCTGT
    GAAAACAAAA CCACTGTCGT CTCTCTGAAG AGTACGGCTA AGAGACTTGA CTCTGTGATT
    GAAAAAACAT CCAAGTGGTC CAACTTCACC AAAGAAGAGA CCTCGACGCT GGCCACAGTC
    TTGCTGGAGA GCGTGGAAAG CACCACGTTG GCAGCTTTTC TGAAACCCTC AGCGAATGTC
    AGTCAGACTA TTCAGACGAA ACACTTAGAT ATCGAGAGCA AAGTGATTGA CAAAGAATGC
    ACTAAAGAGA ATGAGACATT TAAGTTGAAA GCAAAAGGGG ATGAAATGAA GATTTGGTGT
    TCCACAATCA AAGAATCTGA ATCCACAGGG ATCAACGGGG TGGCTTTCGT TTCCTTTTCG
    GGTATGGAGT CTATTTTGGA TGAGCGCTTC TTGAAAGATC TTCAGATCCC CTGGGCCAGC
    TCCAAGAAGA AGCTGAAGAT GAATTCTCGA GTTGTTGGGG GCATCATAAC AGGAGGGAAG
    AAAGACGGGT TCTCTAAACC AGTAATCTAT ACTCTGGAAA ACATTAAGCC AAAGCAGGAT
    TTTGAGAGTG CTATCTGCGT TTCCTGGAAA CCGGACGTGG AGGGAGGCAG ATGGACACCA
    TCAGGCTGTG TGCTCCTTGA AGCTTCCAAG ACACATACTG TCTGCGGCTG TAACCAAATG
    GTGAACTTGG CTGTCATCAT GGCTTCTGGG GAGGTCACGA TGGAGTTCAC CCTGTACCTC
    ATTAGTCACG TGGGCACGAT TATCTCACTG GTGTGTCTTG CCATGGCCAT CATCACCTTC
    CTTCTGTGCC GCACCCTCCG AAACCAGAAC ACCTACATCC ACCTGCATCT CTGCATATGC
    CTCTTCTTGG CCAAGATGCT GTTTCTCACT GGTGTGGACA AGACCGACAA CCAGATGGGC
    TGCGCCCTCA TCGCAGGCTT TCTGCACTAC CTTTTCCTTG CGTGCTTCTT CTGGATGCTG
    GTGGAGGCTG TGATGCTCTT CCTCATGGTC AGGAACCTGA AAGTGGTGAA TTACTTCAGT
    TCTCGAAACA TCAAGATGCT GTATCTCTGC GCCTTTGGCT ATGGGCTCCC GGGGCTGGTG
    GTGGCTGTTT CTGCTGGCTT ACAACCACAG GGTTATGGGA TGTATAATCG CTGCTGGCTG
    AATACAGAAA CGGGGTTCAT CTGGAGCTTC CTGGGGCCAG TGTGCACAAT TATAGTGGTC
    AATTCCATCC TCCTGACTTG GACACTATGT ATCCTGAGGC AGAAGCTTTC TAGTGTCAAT
    GCAGAAGTCT CCACACTCAA AGATACCAGG TTGCTGACCT TCAAAGCCTT TGCCCAGATC
    TTCATCTTGG GGTGCTCCTG GGTGTTAGGC ATTTTCCAAA TTGGACCCAT GGCCAATATC
    ATGGCTTATC TGTTCACCAT CATCAACAGC TTGCAGGGGG CCTTCATCTT CCTCATTCAT
    TGTGTGCTCA GCCGCCAGGT ACGGGAAGAA TACAGAAGAT GCACGACCAG GAAGACCAAA
    CCCAGCTCTG AGACCCAGAC CTCAGGGATC TTACTGTCAT CTGTTCCCTC CACTTCCAAA
    ACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47162
    Clone ID Related Accession (Same CDS sequence) XM_010798270.2 , XM_010806938.2 , XM_010806938.3
    Accession Version XM_010806938.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATATAG ATGAATGTTC TCAAAAGCCC CCACCTTGTG GTCCTAACTC AAGGTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCCTCTCC CACGGGAAAT
    AACTGGATCT CTGGAAAGCC AGGTCATTTC ACCTGTACAG ACATTAATGA ATGCCTCTCC
    CATGGGGTCT GCCCTGAGCA TTCTGAGTGC ACCAACTCTT TGGGAAGCTA CCGTTGCAGC
    TGCAAAGTTG GATTCACCTC TGGAAACTCC ACCTGTGAAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCCACTTGT CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC GAGAAGTGGA AGGAAAAGTT TCCAGGGGCC AGGAGAGATG
    TGTCAAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC AACTTGCCAC
    AACAGTGTTG GAAGCTACTC TTGTGCCTGC AACTCAGGAT ATATATCCAG AAGTGGAAAG
    AAGAGTTTCC AGGGACCAGG GGAGACATGT CAAGATATAG ACGAATGTTC TCAAAAGCCC
    CCACCTTGTG GTCCTAACTC AAGGTGCAGA AACCTGCCAG GAAGGTATGA GTGCAGCTGT
    TTGACTGGTT TCTCCTCTCC CACGGGAAAT AACTGGATCT CTGGAAAGCC AGGTCATTTC
    ACCTGTACAG ACATTAATGA ATGCCTCTCC CATGGGGTCT GCCCTGAGCA TTCTGAGTGC
    ACCAACTCTT TGGGAAGCTA CCGTTGCAGC TGCAAAGTTG GATTCACCTC TGGAAACTCC
    ACCTGTGAAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGT
    CACAACAGTC TTGGAAGCTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC CAGAAGTGGA
    AAGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATA TAGATGAATG TTCTCAAACA
    CCCCCAACTT GTGGTCCTAA CTCAAGCTGC AGAAACCTGC CAGGAAGGTA TGAGTGCAGC
    TGTTTGACTG GTTTCTCTTC TCCCACTGGA AATAATTGGA TCCCTGGAAA GTCAGGTCAT
    TTTACCTGCA CAGATGTGGA TGAATGTGCC AATCCCAGAA GTTGCCCAGA GCATTCGACT
    TGCCACAACA GTCTTGGAAG CTACTCTTGT GTCTGCAACC CGGGTTTTCA ATCCAGAAGT
    GGAAAGAAGA ATTTCCAGGG ACCAGGAGAG ACATGTGAAG ATGTGGACGA ATGTTCCAGG
    AACTCAACTC TTTGTGGTCC CAGTTCTGTC TGCACCAACA TCCCAGGAAA ATACAGCTGC
    TCCTGCTTGC CGGGGTTCTT TTCACCTGGT GTGTGGAGCC CTGAAAAACC TGAGGCTTTC
    AAGTGTGCAG ATATTGACGA GTGCCTTCAG GATCCCTCAC GATGCGGCCC AGATTCTGTC
    TGCACCAACA CCCTGGGCTC CTACAGCTGT GGCTGCGTTG TAGGCTTTCA TCCCAATCCA
    GAAGGCTCCT GGAAACATGG CAACTTCAGC TGCCAAAGGA TTCCCTTCAA GTGTAAAGAT
    GATGTGATAC CCAGCAACAT GCAGGTCCAG TTATGCCATG CGGGAGCAGC GGTGGAGCCT
    AAATATGTTT CCTTTTGTGC GCTGATGAAT GCCACCCTCA GCGTTCTGGA CAATGTCTGT
    GAAAACAAAA CCACTGTCGT CTCTCTGAAG AGTACGGCTA AGAGACTTGA CTCTGTGATT
    GAAAAAACAT CCAAGTGGTC CAACTTCACC AAAGAAGAGA CCTCGACGCT GGCCACAGTC
    TTGCTGGAGA GCGTGGAAAG CACCACGTTG GCAGCTTTTC TGAAACCCTC AGCGAATGTC
    AGTCAGACTA TTCAGACGAA ACACTTAGAT ATCGAGAGCA AAGTGATTGA CAAAGAATGC
    ACTAAAGAGA ATGAGACATT TAAGTTGAAA GCAAAAGGGG ATGAAATGAA GATTTGGTGT
    TCCACAATCA AAGAATCTGA ATCCACAGGG ATCAACGGGG TGGCTTTCGT TTCCTTTTCG
    GGTATGGAGT CTATTTTGGA TGAGCGCTTC TTGAAAGATC TTCAGATCCC CTGGGCCAGC
    TCCAAGAAGA AGCTGAAGAT GAATTCTCGA GTTGTTGGGG GCATCATAAC AGGAGGGAAG
    AAAGACGGGT TCTCTAAACC AGTAATCTAT ACTCTGGAAA ACATTAAGCC AAAGCAGGAT
    TTTGAGAGTG CTATCTGCGT TTCCTGGAAA CCGGACGTGG AGGGAGGCAG ATGGACACCA
    TCAGGCTGTG TGCTCCTTGA AGCTTCCAAG ACACATACTG TCTGCGGCTG TAACCAAATG
    GTGAACTTGG CTGTCATCAT GGCTTCTGGG GAGGTCACGA TGGAGTTCAC CCTGTACCTC
    ATTAGTCACG TGGGCACGAT TATCTCACTG GTGTGTCTTG CCATGGCCAT CATCACCTTC
    CTTCTGTGCC GCACCCTCCG AAACCAGAAC ACCTACATCC ACCTGCATCT CTGCATATGC
    CTCTTCTTGG CCAAGATGCT GTTTCTCACT GGTGTGGACA AGACCGACAA CCAGATGGGC
    TGCGCCCTCA TCGCAGGCTT TCTGCACTAC CTTTTCCTTG CGTGCTTCTT CTGGATGCTG
    GTGGAGGCTG TGATGCTCTT CCTCATGGTC AGGAACCTGA AAGTGGTGAA TTACTTCAGT
    TCTCGAAACA TCAAGATGCT GTATCTCTGC GCCTTTGGCT ATGGGCTCCC GGGGCTGGTG
    GTGGCTGTTT CTGCTGGCTT ACAACCACAG GGTTATGGGA TGTATAATCG CTGCTGGCTG
    AATACAGAAA CGGGGTTCAT CTGGAGCTTC CTGGGGCCAG TGTGCACAAT TATAGTGGTC
    AATTCCATCC TCCTGACTTG GACACTATGT ATCCTGAGGC AGAAGCTTTC TAGTGTCAAT
    GCAGAAGTCT CCACACTCAA AGATACCAGG TTGCTGACCT TCAAAGCCTT TGCCCAGATC
    TTCATCTTGG GGTGCTCCTG GGTGTTAGGC ATTTTCCAAA TTGGACCCAT GGCCAATATC
    ATGGCTTATC TGTTCACCAT CATCAACAGC TTGCAGGGGG CCTTCATCTT CCTCATTCAT
    TGTGTGCTCA GCCGCCAGGT ACGGGAAGAA TACAGAAGAT GCACGACCAG GAAGACCAAA
    CCCAGCTCTG AGACCCAGAC CTCAGGGATC TTACTGTCAT CTGTTCCCTC CACTTCCAAA
    ACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805240.1
    CDS103..4131
    Translation

    Target ORF information:

    RefSeq Version XM_010806938.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806938.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATATAG ATGAATGTTC TCAAAAGCCC CCACCTTGTG GTCCTAACTC AAGGTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCCTCTCC CACGGGAAAT
    AACTGGATCT CTGGAAAGCC AGGTCATTTC ACCTGTACAG ACATTAATGA ATGCCTCTCC
    CATGGGGTCT GCCCTGAGCA TTCTGAGTGC ACCAACTCTT TGGGAAGCTA CCGTTGCAGC
    TGCAAAGTTG GATTCACCTC TGGAAACTCC ACCTGTGAAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCCACTTGT CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC GAGAAGTGGA AGGAAAAGTT TCCAGGGGCC AGGAGAGATG
    TGTCAAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC AACTTGCCAC
    AACAGTGTTG GAAGCTACTC TTGTGCCTGC AACTCAGGAT ATATATCCAG AAGTGGAAAG
    AAGAGTTTCC AGGGACCAGG GGAGACATGT CAAGATATAG ACGAATGTTC TCAAAAGCCC
    CCACCTTGTG GTCCTAACTC AAGGTGCAGA AACCTGCCAG GAAGGTATGA GTGCAGCTGT
    TTGACTGGTT TCTCCTCTCC CACGGGAAAT AACTGGATCT CTGGAAAGCC AGGTCATTTC
    ACCTGTACAG ACATTAATGA ATGCCTCTCC CATGGGGTCT GCCCTGAGCA TTCTGAGTGC
    ACCAACTCTT TGGGAAGCTA CCGTTGCAGC TGCAAAGTTG GATTCACCTC TGGAAACTCC
    ACCTGTGAAG ATGTGGATGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGT
    CACAACAGTC TTGGAAGCTA CTCTTGTGTC TGCAACCCGG GTTTTCAATC CAGAAGTGGA
    AAGAAAAGTT TCCAGGGGCC AGGAGAGATG TGTCAAGATA TAGATGAATG TTCTCAAACA
    CCCCCAACTT GTGGTCCTAA CTCAAGCTGC AGAAACCTGC CAGGAAGGTA TGAGTGCAGC
    TGTTTGACTG GTTTCTCTTC TCCCACTGGA AATAATTGGA TCCCTGGAAA GTCAGGTCAT
    TTTACCTGCA CAGATGTGGA TGAATGTGCC AATCCCAGAA GTTGCCCAGA GCATTCGACT
    TGCCACAACA GTCTTGGAAG CTACTCTTGT GTCTGCAACC CGGGTTTTCA ATCCAGAAGT
    GGAAAGAAGA ATTTCCAGGG ACCAGGAGAG ACATGTGAAG ATGTGGACGA ATGTTCCAGG
    AACTCAACTC TTTGTGGTCC CAGTTCTGTC TGCACCAACA TCCCAGGAAA ATACAGCTGC
    TCCTGCTTGC CGGGGTTCTT TTCACCTGGT GTGTGGAGCC CTGAAAAACC TGAGGCTTTC
    AAGTGTGCAG ATATTGACGA GTGCCTTCAG GATCCCTCAC GATGCGGCCC AGATTCTGTC
    TGCACCAACA CCCTGGGCTC CTACAGCTGT GGCTGCGTTG TAGGCTTTCA TCCCAATCCA
    GAAGGCTCCT GGAAACATGG CAACTTCAGC TGCCAAAGGA TTCCCTTCAA GTGTAAAGAT
    GATGTGATAC CCAGCAACAT GCAGGTCCAG TTATGCCATG CGGGAGCAGC GGTGGAGCCT
    AAATATGTTT CCTTTTGTGC GCTGATGAAT GCCACCCTCA GCGTTCTGGA CAATGTCTGT
    GAAAACAAAA CCACTGTCGT CTCTCTGAAG AGTACGGCTA AGAGACTTGA CTCTGTGATT
    GAAAAAACAT CCAAGTGGTC CAACTTCACC AAAGAAGAGA CCTCGACGCT GGCCACAGTC
    TTGCTGGAGA GCGTGGAAAG CACCACGTTG GCAGCTTTTC TGAAACCCTC AGCGAATGTC
    AGTCAGACTA TTCAGACGAA ACACTTAGAT ATCGAGAGCA AAGTGATTGA CAAAGAATGC
    ACTAAAGAGA ATGAGACATT TAAGTTGAAA GCAAAAGGGG ATGAAATGAA GATTTGGTGT
    TCCACAATCA AAGAATCTGA ATCCACAGGG ATCAACGGGG TGGCTTTCGT TTCCTTTTCG
    GGTATGGAGT CTATTTTGGA TGAGCGCTTC TTGAAAGATC TTCAGATCCC CTGGGCCAGC
    TCCAAGAAGA AGCTGAAGAT GAATTCTCGA GTTGTTGGGG GCATCATAAC AGGAGGGAAG
    AAAGACGGGT TCTCTAAACC AGTAATCTAT ACTCTGGAAA ACATTAAGCC AAAGCAGGAT
    TTTGAGAGTG CTATCTGCGT TTCCTGGAAA CCGGACGTGG AGGGAGGCAG ATGGACACCA
    TCAGGCTGTG TGCTCCTTGA AGCTTCCAAG ACACATACTG TCTGCGGCTG TAACCAAATG
    GTGAACTTGG CTGTCATCAT GGCTTCTGGG GAGGTCACGA TGGAGTTCAC CCTGTACCTC
    ATTAGTCACG TGGGCACGAT TATCTCACTG GTGTGTCTTG CCATGGCCAT CATCACCTTC
    CTTCTGTGCC GCACCCTCCG AAACCAGAAC ACCTACATCC ACCTGCATCT CTGCATATGC
    CTCTTCTTGG CCAAGATGCT GTTTCTCACT GGTGTGGACA AGACCGACAA CCAGATGGGC
    TGCGCCCTCA TCGCAGGCTT TCTGCACTAC CTTTTCCTTG CGTGCTTCTT CTGGATGCTG
    GTGGAGGCTG TGATGCTCTT CCTCATGGTC AGGAACCTGA AAGTGGTGAA TTACTTCAGT
    TCTCGAAACA TCAAGATGCT GTATCTCTGC GCCTTTGGCT ATGGGCTCCC GGGGCTGGTG
    GTGGCTGTTT CTGCTGGCTT ACAACCACAG GGTTATGGGA TGTATAATCG CTGCTGGCTG
    AATACAGAAA CGGGGTTCAT CTGGAGCTTC CTGGGGCCAG TGTGCACAAT TATAGTGGTC
    AATTCCATCC TCCTGACTTG GACACTATGT ATCCTGAGGC AGAAGCTTTC TAGTGTCAAT
    GCAGAAGTCT CCACACTCAA AGATACCAGG TTGCTGACCT TCAAAGCCTT TGCCCAGATC
    TTCATCTTGG GGTGCTCCTG GGTGTTAGGC ATTTTCCAAA TTGGACCCAT GGCCAATATC
    ATGGCTTATC TGTTCACCAT CATCAACAGC TTGCAGGGGG CCTTCATCTT CCTCATTCAT
    TGTGTGCTCA GCCGCCAGGT ACGGGAAGAA TACAGAAGAT GCACGACCAG GAAGACCAAA
    CCCAGCTCTG AGACCCAGAC CTCAGGGATC TTACTGTCAT CTGTTCCCTC CACTTCCAAA
    ACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47166
    Clone ID Related Accession (Same CDS sequence) XM_010806933.2 , XM_010798265.2
    Accession Version XM_010806933.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805235.1
    CDS75..4397
    Translation

    Target ORF information:

    RefSeq Version XM_010806933.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47166
    Clone ID Related Accession (Same CDS sequence) XM_010806933.2 , XM_010798265.2
    Accession Version XM_010798265.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796567.2
    CDS75..4397
    Translation

    Target ORF information:

    RefSeq Version XM_010798265.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798265.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AACACCCCCA
    ACTTGTGGTC CTAACTCAAG CTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAT TGGATCCCTG GAAAGTCAGG TCATTTTACC
    TGCACAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC GACTTGCCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCCAG AAGTGGAAAG
    AAGAATTTCC AGGGACCAGG AGAGACATGT GAAGATGTGG ACGAATGTTC CAGGAACTCA
    ACTCTTTGTG GTCCCAGTTC TGTCTGCACC AACATCCCAG GAAAATACAG CTGCTCCTGC
    TTGCCGGGGT TCTTTTCACC TGGTGTGTGG AGCCCTGAAA AACCTGAGGC TTTCAAGTGT
    GCAGATATTG ACGAGTGCCT TCAGGATCCC TCACGATGCG GCCCAGATTC TGTCTGCACC
    AACACCCTGG GCTCCTACAG CTGTGGCTGC GTTGTAGGCT TTCATCCCAA TCCAGAAGGC
    TCCTGGAAAC ATGGCAACTT CAGCTGCCAA AGGATTCCCT TCAAGTGTAA AGATGATGTG
    ATACCCAGCA ACATGCAGGT CCAGTTATGC CATGCGGGAG CAGCGGTGGA GCCTAAATAT
    GTTTCCTTTT GTGCGCTGAT GAATGCCACC CTCAGCGTTC TGGACAATGT CTGTGAAAAC
    AAAACCACTG TCGTCTCTCT GAAGAGTACG GCTAAGAGAC TTGACTCTGT GATTGAAAAA
    ACATCCAAGT GGTCCAACTT CACCAAAGAA GAGACCTCGA CGCTGGCCAC AGTCTTGCTG
    GAGAGCGTGG AAAGCACCAC GTTGGCAGCT TTTCTGAAAC CCTCAGCGAA TGTCAGTCAG
    ACTATTCAGA CGAAACACTT AGATATCGAG AGCAAAGTGA TTGACAAAGA ATGCACTAAA
    GAGAATGAGA CATTTAAGTT GAAAGCAAAA GGGGATGAAA TGAAGATTTG GTGTTCCACA
    ATCAAAGAAT CTGAATCCAC AGGGATCAAC GGGGTGGCTT TCGTTTCCTT TTCGGGTATG
    GAGTCTATTT TGGATGAGCG CTTCTTGAAA GATCTTCAGA TCCCCTGGGC CAGCTCCAAG
    AAGAAGCTGA AGATGAATTC TCGAGTTGTT GGGGGCATCA TAACAGGAGG GAAGAAAGAC
    GGGTTCTCTA AACCAGTAAT CTATACTCTG GAAAACATTA AGCCAAAGCA GGATTTTGAG
    AGTGCTATCT GCGTTTCCTG GAAACCGGAC GTGGAGGGAG GCAGATGGAC ACCATCAGGC
    TGTGTGCTCC TTGAAGCTTC CAAGACACAT ACTGTCTGCG GCTGTAACCA AATGGTGAAC
    TTGGCTGTCA TCATGGCTTC TGGGGAGGTC ACGATGGAGT TCACCCTGTA CCTCATTAGT
    CACGTGGGCA CGATTATCTC ACTGGTGTGT CTTGCCATGG CCATCATCAC CTTCCTTCTG
    TGCCGCACCC TCCGAAACCA GAACACCTAC ATCCACCTGC ATCTCTGCAT ATGCCTCTTC
    TTGGCCAAGA TGCTGTTTCT CACTGGTGTG GACAAGACCG ACAACCAGAT GGGCTGCGCC
    CTCATCGCAG GCTTTCTGCA CTACCTTTTC CTTGCGTGCT TCTTCTGGAT GCTGGTGGAG
    GCTGTGATGC TCTTCCTCAT GGTCAGGAAC CTGAAAGTGG TGAATTACTT CAGTTCTCGA
    AACATCAAGA TGCTGTATCT CTGCGCCTTT GGCTATGGGC TCCCGGGGCT GGTGGTGGCT
    GTTTCTGCTG GCTTACAACC ACAGGGTTAT GGGATGTATA ATCGCTGCTG GCTGAATACA
    GAAACGGGGT TCATCTGGAG CTTCCTGGGG CCAGTGTGCA CAATTATAGT GGTCAATTCC
    ATCCTCCTGA CTTGGACACT ATGTATCCTG AGGCAGAAGC TTTCTAGTGT CAATGCAGAA
    GTCTCCACAC TCAAAGATAC CAGGTTGCTG ACCTTCAAAG CCTTTGCCCA GATCTTCATC
    TTGGGGTGCT CCTGGGTGTT AGGCATTTTC CAAATTGGAC CCATGGCCAA TATCATGGCT
    TATCTGTTCA CCATCATCAA CAGCTTGCAG GGGGCCTTCA TCTTCCTCAT TCATTGTGTG
    CTCAGCCGCC AGGTACGGGA AGAATACAGA AGATGCACGA CCAGGAAGAC CAAACCCAGC
    TCTGAGACCC AGACCTCAGG GATCTTACTG TCATCTGTTC CCTCCACTTC CAAAACGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47168
    Clone ID Related Accession (Same CDS sequence) XM_010798268.2 , XM_010806936.2
    Accession Version XM_010798268.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796570.2
    CDS75..4241
    Translation

    Target ORF information:

    RefSeq Version XM_010798268.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798268.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47168
    Clone ID Related Accession (Same CDS sequence) XM_010798268.2 , XM_010806936.2
    Accession Version XM_010806936.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805238.1
    CDS75..4241
    Translation

    Target ORF information:

    RefSeq Version XM_010806936.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806936.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT ATAGACGAAT GTTCTCAAAA GCCCCCACCT
    TGTGGTCCTA ACTCAAGGTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCCT CTCCCACGGG AAATAACTGG ATCTCTGGAA AGCCAGGTCA TTTCACCTGT
    ACAGACATTA ATGAATGCCT CTCCCATGGG GTCTGCCCTG AGCATTCTGA GTGCACCAAC
    TCTTTGGGAA GCTACCGTTG CAGCTGCAAA GTTGGATTCA CCTCTGGAAA CTCCACCTGT
    GAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCCAC TTGTCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47169
    Clone ID Related Accession (Same CDS sequence) XM_010798269.2 , XM_010806937.2
    Accession Version XM_010798269.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796571.2
    CDS75..4121
    Translation

    Target ORF information:

    RefSeq Version XM_010798269.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47169
    Clone ID Related Accession (Same CDS sequence) XM_010798269.2 , XM_010806937.2
    Accession Version XM_010806937.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805239.1
    CDS75..4121
    Translation

    Target ORF information:

    RefSeq Version XM_010806937.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47170
    Clone ID Related Accession (Same CDS sequence) XM_010798271.2 , XM_010806939.2
    Accession Version XM_010798271.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796573.2
    CDS75..3974
    Translation

    Target ORF information:

    RefSeq Version XM_010798271.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798271.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47170
    Clone ID Related Accession (Same CDS sequence) XM_010798271.2 , XM_010806939.2
    Accession Version XM_010806939.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805241.1
    CDS75..3974
    Translation

    Target ORF information:

    RefSeq Version XM_010806939.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47171
    Clone ID Related Accession (Same CDS sequence) XM_010798272.2 , XM_010806940.2
    Accession Version XM_010798272.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CGACTTGCCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAGAATTTC CAGGGACCAG GAGAGACATG TGAAGATGTG
    GACGAATGTT CCAGGAACTC AACTCTTTGT GGTCCCAGTT CTGTCTGCAC CAACATCCCA
    GGAAAATACA GCTGCTCCTG CTTGCCGGGG TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA
    AAACCTGAGG CTTTCAAGTG TGCAGATATT GACGAGTGCC TTCAGGATCC CTCACGATGC
    GGCCCAGATT CTGTCTGCAC CAACACCCTG GGCTCCTACA GCTGTGGCTG CGTTGTAGGC
    TTTCATCCCA ATCCAGAAGG CTCCTGGAAA CATGGCAACT TCAGCTGCCA AAGGATTCCC
    TTCAAGTGTA AAGATGATGT GATACCCAGC AACATGCAGG TCCAGTTATG CCATGCGGGA
    GCAGCGGTGG AGCCTAAATA TGTTTCCTTT TGTGCGCTGA TGAATGCCAC CCTCAGCGTT
    CTGGACAATG TCTGTGAAAA CAAAACCACT GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA
    CTTGACTCTG TGATTGAAAA AACATCCAAG TGGTCCAACT TCACCAAAGA AGAGACCTCG
    ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG GAAAGCACCA CGTTGGCAGC TTTTCTGAAA
    CCCTCAGCGA ATGTCAGTCA GACTATTCAG ACGAAACACT TAGATATCGA GAGCAAAGTG
    ATTGACAAAG AATGCACTAA AGAGAATGAG ACATTTAAGT TGAAAGCAAA AGGGGATGAA
    ATGAAGATTT GGTGTTCCAC AATCAAAGAA TCTGAATCCA CAGGGATCAA CGGGGTGGCT
    TTCGTTTCCT TTTCGGGTAT GGAGTCTATT TTGGATGAGC GCTTCTTGAA AGATCTTCAG
    ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG AAGATGAATT CTCGAGTTGT TGGGGGCATC
    ATAACAGGAG GGAAGAAAGA CGGGTTCTCT AAACCAGTAA TCTATACTCT GGAAAACATT
    AAGCCAAAGC AGGATTTTGA GAGTGCTATC TGCGTTTCCT GGAAACCGGA CGTGGAGGGA
    GGCAGATGGA CACCATCAGG CTGTGTGCTC CTTGAAGCTT CCAAGACACA TACTGTCTGC
    GGCTGTAACC AAATGGTGAA CTTGGCTGTC ATCATGGCTT CTGGGGAGGT CACGATGGAG
    TTCACCCTGT ACCTCATTAG TCACGTGGGC ACGATTATCT CACTGGTGTG TCTTGCCATG
    GCCATCATCA CCTTCCTTCT GTGCCGCACC CTCCGAAACC AGAACACCTA CATCCACCTG
    CATCTCTGCA TATGCCTCTT CTTGGCCAAG ATGCTGTTTC TCACTGGTGT GGACAAGACC
    GACAACCAGA TGGGCTGCGC CCTCATCGCA GGCTTTCTGC ACTACCTTTT CCTTGCGTGC
    TTCTTCTGGA TGCTGGTGGA GGCTGTGATG CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG
    GTGAATTACT TCAGTTCTCG AAACATCAAG ATGCTGTATC TCTGCGCCTT TGGCTATGGG
    CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT GGCTTACAAC CACAGGGTTA TGGGATGTAT
    AATCGCTGCT GGCTGAATAC AGAAACGGGG TTCATCTGGA GCTTCCTGGG GCCAGTGTGC
    ACAATTATAG TGGTCAATTC CATCCTCCTG ACTTGGACAC TATGTATCCT GAGGCAGAAG
    CTTTCTAGTG TCAATGCAGA AGTCTCCACA CTCAAAGATA CCAGGTTGCT GACCTTCAAA
    GCCTTTGCCC AGATCTTCAT CTTGGGGTGC TCCTGGGTGT TAGGCATTTT CCAAATTGGA
    CCCATGGCCA ATATCATGGC TTATCTGTTC ACCATCATCA ACAGCTTGCA GGGGGCCTTC
    ATCTTCCTCA TTCATTGTGT GCTCAGCCGC CAGGTACGGG AAGAATACAG AAGATGCACG
    ACCAGGAAGA CCAAACCCAG CTCTGAGACC CAGACCTCAG GGATCTTACT GTCATCTGTT
    CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796574.2
    CDS75..3818
    Translation

    Target ORF information:

    RefSeq Version XM_010798272.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798272.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CGACTTGCCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAGAATTTC CAGGGACCAG GAGAGACATG TGAAGATGTG
    GACGAATGTT CCAGGAACTC AACTCTTTGT GGTCCCAGTT CTGTCTGCAC CAACATCCCA
    GGAAAATACA GCTGCTCCTG CTTGCCGGGG TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA
    AAACCTGAGG CTTTCAAGTG TGCAGATATT GACGAGTGCC TTCAGGATCC CTCACGATGC
    GGCCCAGATT CTGTCTGCAC CAACACCCTG GGCTCCTACA GCTGTGGCTG CGTTGTAGGC
    TTTCATCCCA ATCCAGAAGG CTCCTGGAAA CATGGCAACT TCAGCTGCCA AAGGATTCCC
    TTCAAGTGTA AAGATGATGT GATACCCAGC AACATGCAGG TCCAGTTATG CCATGCGGGA
    GCAGCGGTGG AGCCTAAATA TGTTTCCTTT TGTGCGCTGA TGAATGCCAC CCTCAGCGTT
    CTGGACAATG TCTGTGAAAA CAAAACCACT GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA
    CTTGACTCTG TGATTGAAAA AACATCCAAG TGGTCCAACT TCACCAAAGA AGAGACCTCG
    ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG GAAAGCACCA CGTTGGCAGC TTTTCTGAAA
    CCCTCAGCGA ATGTCAGTCA GACTATTCAG ACGAAACACT TAGATATCGA GAGCAAAGTG
    ATTGACAAAG AATGCACTAA AGAGAATGAG ACATTTAAGT TGAAAGCAAA AGGGGATGAA
    ATGAAGATTT GGTGTTCCAC AATCAAAGAA TCTGAATCCA CAGGGATCAA CGGGGTGGCT
    TTCGTTTCCT TTTCGGGTAT GGAGTCTATT TTGGATGAGC GCTTCTTGAA AGATCTTCAG
    ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG AAGATGAATT CTCGAGTTGT TGGGGGCATC
    ATAACAGGAG GGAAGAAAGA CGGGTTCTCT AAACCAGTAA TCTATACTCT GGAAAACATT
    AAGCCAAAGC AGGATTTTGA GAGTGCTATC TGCGTTTCCT GGAAACCGGA CGTGGAGGGA
    GGCAGATGGA CACCATCAGG CTGTGTGCTC CTTGAAGCTT CCAAGACACA TACTGTCTGC
    GGCTGTAACC AAATGGTGAA CTTGGCTGTC ATCATGGCTT CTGGGGAGGT CACGATGGAG
    TTCACCCTGT ACCTCATTAG TCACGTGGGC ACGATTATCT CACTGGTGTG TCTTGCCATG
    GCCATCATCA CCTTCCTTCT GTGCCGCACC CTCCGAAACC AGAACACCTA CATCCACCTG
    CATCTCTGCA TATGCCTCTT CTTGGCCAAG ATGCTGTTTC TCACTGGTGT GGACAAGACC
    GACAACCAGA TGGGCTGCGC CCTCATCGCA GGCTTTCTGC ACTACCTTTT CCTTGCGTGC
    TTCTTCTGGA TGCTGGTGGA GGCTGTGATG CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG
    GTGAATTACT TCAGTTCTCG AAACATCAAG ATGCTGTATC TCTGCGCCTT TGGCTATGGG
    CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT GGCTTACAAC CACAGGGTTA TGGGATGTAT
    AATCGCTGCT GGCTGAATAC AGAAACGGGG TTCATCTGGA GCTTCCTGGG GCCAGTGTGC
    ACAATTATAG TGGTCAATTC CATCCTCCTG ACTTGGACAC TATGTATCCT GAGGCAGAAG
    CTTTCTAGTG TCAATGCAGA AGTCTCCACA CTCAAAGATA CCAGGTTGCT GACCTTCAAA
    GCCTTTGCCC AGATCTTCAT CTTGGGGTGC TCCTGGGTGT TAGGCATTTT CCAAATTGGA
    CCCATGGCCA ATATCATGGC TTATCTGTTC ACCATCATCA ACAGCTTGCA GGGGGCCTTC
    ATCTTCCTCA TTCATTGTGT GCTCAGCCGC CAGGTACGGG AAGAATACAG AAGATGCACG
    ACCAGGAAGA CCAAACCCAG CTCTGAGACC CAGACCTCAG GGATCTTACT GTCATCTGTT
    CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47171
    Clone ID Related Accession (Same CDS sequence) XM_010798272.2 , XM_010806940.2
    Accession Version XM_010806940.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CGACTTGCCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAGAATTTC CAGGGACCAG GAGAGACATG TGAAGATGTG
    GACGAATGTT CCAGGAACTC AACTCTTTGT GGTCCCAGTT CTGTCTGCAC CAACATCCCA
    GGAAAATACA GCTGCTCCTG CTTGCCGGGG TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA
    AAACCTGAGG CTTTCAAGTG TGCAGATATT GACGAGTGCC TTCAGGATCC CTCACGATGC
    GGCCCAGATT CTGTCTGCAC CAACACCCTG GGCTCCTACA GCTGTGGCTG CGTTGTAGGC
    TTTCATCCCA ATCCAGAAGG CTCCTGGAAA CATGGCAACT TCAGCTGCCA AAGGATTCCC
    TTCAAGTGTA AAGATGATGT GATACCCAGC AACATGCAGG TCCAGTTATG CCATGCGGGA
    GCAGCGGTGG AGCCTAAATA TGTTTCCTTT TGTGCGCTGA TGAATGCCAC CCTCAGCGTT
    CTGGACAATG TCTGTGAAAA CAAAACCACT GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA
    CTTGACTCTG TGATTGAAAA AACATCCAAG TGGTCCAACT TCACCAAAGA AGAGACCTCG
    ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG GAAAGCACCA CGTTGGCAGC TTTTCTGAAA
    CCCTCAGCGA ATGTCAGTCA GACTATTCAG ACGAAACACT TAGATATCGA GAGCAAAGTG
    ATTGACAAAG AATGCACTAA AGAGAATGAG ACATTTAAGT TGAAAGCAAA AGGGGATGAA
    ATGAAGATTT GGTGTTCCAC AATCAAAGAA TCTGAATCCA CAGGGATCAA CGGGGTGGCT
    TTCGTTTCCT TTTCGGGTAT GGAGTCTATT TTGGATGAGC GCTTCTTGAA AGATCTTCAG
    ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG AAGATGAATT CTCGAGTTGT TGGGGGCATC
    ATAACAGGAG GGAAGAAAGA CGGGTTCTCT AAACCAGTAA TCTATACTCT GGAAAACATT
    AAGCCAAAGC AGGATTTTGA GAGTGCTATC TGCGTTTCCT GGAAACCGGA CGTGGAGGGA
    GGCAGATGGA CACCATCAGG CTGTGTGCTC CTTGAAGCTT CCAAGACACA TACTGTCTGC
    GGCTGTAACC AAATGGTGAA CTTGGCTGTC ATCATGGCTT CTGGGGAGGT CACGATGGAG
    TTCACCCTGT ACCTCATTAG TCACGTGGGC ACGATTATCT CACTGGTGTG TCTTGCCATG
    GCCATCATCA CCTTCCTTCT GTGCCGCACC CTCCGAAACC AGAACACCTA CATCCACCTG
    CATCTCTGCA TATGCCTCTT CTTGGCCAAG ATGCTGTTTC TCACTGGTGT GGACAAGACC
    GACAACCAGA TGGGCTGCGC CCTCATCGCA GGCTTTCTGC ACTACCTTTT CCTTGCGTGC
    TTCTTCTGGA TGCTGGTGGA GGCTGTGATG CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG
    GTGAATTACT TCAGTTCTCG AAACATCAAG ATGCTGTATC TCTGCGCCTT TGGCTATGGG
    CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT GGCTTACAAC CACAGGGTTA TGGGATGTAT
    AATCGCTGCT GGCTGAATAC AGAAACGGGG TTCATCTGGA GCTTCCTGGG GCCAGTGTGC
    ACAATTATAG TGGTCAATTC CATCCTCCTG ACTTGGACAC TATGTATCCT GAGGCAGAAG
    CTTTCTAGTG TCAATGCAGA AGTCTCCACA CTCAAAGATA CCAGGTTGCT GACCTTCAAA
    GCCTTTGCCC AGATCTTCAT CTTGGGGTGC TCCTGGGTGT TAGGCATTTT CCAAATTGGA
    CCCATGGCCA ATATCATGGC TTATCTGTTC ACCATCATCA ACAGCTTGCA GGGGGCCTTC
    ATCTTCCTCA TTCATTGTGT GCTCAGCCGC CAGGTACGGG AAGAATACAG AAGATGCACG
    ACCAGGAAGA CCAAACCCAG CTCTGAGACC CAGACCTCAG GGATCTTACT GTCATCTGTT
    CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805242.1
    CDS75..3818
    Translation

    Target ORF information:

    RefSeq Version XM_010806940.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806940.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    ATAGATGAAT GTTCTCAAAA GCCCCCACCT TGTGGTCCTA ACTCAAGGTG CAGAAACCTG
    CCAGGAAGGT ATGAGTGCAG CTGTTTGACT GGTTTCTCCT CTCCCACGGG AAATAACTGG
    ATCTCTGGAA AGCCAGGTCA TTTCACCTGT ACAGACATTA ATGAATGCCT CTCCCATGGG
    GTCTGCCCTG AGCATTCTGA GTGCACCAAC TCTTTGGGAA GCTACCGTTG CAGCTGCAAA
    GTTGGATTCA CCTCTGGAAA CTCCACCTGT GAAGATGTGG ATGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGTCACAAC AGTCTTGGAA GCTACTCTTG TGTCTGCAAC
    CCGGGTTTTC AATCGAGAAG TGGAAGGAAA AGTTTCCAGG GGCCAGGAGA GATGTGTCAA
    GATGTGGACG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCAACTTG CCACAACAGT
    GTTGGAAGCT ACTCTTGTGC CTGCAACTCA GGATATATAT CCAGAAGTGG AAAGAAGAGT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CGACTTGCCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAGAATTTC CAGGGACCAG GAGAGACATG TGAAGATGTG
    GACGAATGTT CCAGGAACTC AACTCTTTGT GGTCCCAGTT CTGTCTGCAC CAACATCCCA
    GGAAAATACA GCTGCTCCTG CTTGCCGGGG TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA
    AAACCTGAGG CTTTCAAGTG TGCAGATATT GACGAGTGCC TTCAGGATCC CTCACGATGC
    GGCCCAGATT CTGTCTGCAC CAACACCCTG GGCTCCTACA GCTGTGGCTG CGTTGTAGGC
    TTTCATCCCA ATCCAGAAGG CTCCTGGAAA CATGGCAACT TCAGCTGCCA AAGGATTCCC
    TTCAAGTGTA AAGATGATGT GATACCCAGC AACATGCAGG TCCAGTTATG CCATGCGGGA
    GCAGCGGTGG AGCCTAAATA TGTTTCCTTT TGTGCGCTGA TGAATGCCAC CCTCAGCGTT
    CTGGACAATG TCTGTGAAAA CAAAACCACT GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA
    CTTGACTCTG TGATTGAAAA AACATCCAAG TGGTCCAACT TCACCAAAGA AGAGACCTCG
    ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG GAAAGCACCA CGTTGGCAGC TTTTCTGAAA
    CCCTCAGCGA ATGTCAGTCA GACTATTCAG ACGAAACACT TAGATATCGA GAGCAAAGTG
    ATTGACAAAG AATGCACTAA AGAGAATGAG ACATTTAAGT TGAAAGCAAA AGGGGATGAA
    ATGAAGATTT GGTGTTCCAC AATCAAAGAA TCTGAATCCA CAGGGATCAA CGGGGTGGCT
    TTCGTTTCCT TTTCGGGTAT GGAGTCTATT TTGGATGAGC GCTTCTTGAA AGATCTTCAG
    ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG AAGATGAATT CTCGAGTTGT TGGGGGCATC
    ATAACAGGAG GGAAGAAAGA CGGGTTCTCT AAACCAGTAA TCTATACTCT GGAAAACATT
    AAGCCAAAGC AGGATTTTGA GAGTGCTATC TGCGTTTCCT GGAAACCGGA CGTGGAGGGA
    GGCAGATGGA CACCATCAGG CTGTGTGCTC CTTGAAGCTT CCAAGACACA TACTGTCTGC
    GGCTGTAACC AAATGGTGAA CTTGGCTGTC ATCATGGCTT CTGGGGAGGT CACGATGGAG
    TTCACCCTGT ACCTCATTAG TCACGTGGGC ACGATTATCT CACTGGTGTG TCTTGCCATG
    GCCATCATCA CCTTCCTTCT GTGCCGCACC CTCCGAAACC AGAACACCTA CATCCACCTG
    CATCTCTGCA TATGCCTCTT CTTGGCCAAG ATGCTGTTTC TCACTGGTGT GGACAAGACC
    GACAACCAGA TGGGCTGCGC CCTCATCGCA GGCTTTCTGC ACTACCTTTT CCTTGCGTGC
    TTCTTCTGGA TGCTGGTGGA GGCTGTGATG CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG
    GTGAATTACT TCAGTTCTCG AAACATCAAG ATGCTGTATC TCTGCGCCTT TGGCTATGGG
    CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT GGCTTACAAC CACAGGGTTA TGGGATGTAT
    AATCGCTGCT GGCTGAATAC AGAAACGGGG TTCATCTGGA GCTTCCTGGG GCCAGTGTGC
    ACAATTATAG TGGTCAATTC CATCCTCCTG ACTTGGACAC TATGTATCCT GAGGCAGAAG
    CTTTCTAGTG TCAATGCAGA AGTCTCCACA CTCAAAGATA CCAGGTTGCT GACCTTCAAA
    GCCTTTGCCC AGATCTTCAT CTTGGGGTGC TCCTGGGTGT TAGGCATTTT CCAAATTGGA
    CCCATGGCCA ATATCATGGC TTATCTGTTC ACCATCATCA ACAGCTTGCA GGGGGCCTTC
    ATCTTCCTCA TTCATTGTGT GCTCAGCCGC CAGGTACGGG AAGAATACAG AAGATGCACG
    ACCAGGAAGA CCAAACCCAG CTCTGAGACC CAGACCTCAG GGATCTTACT GTCATCTGTT
    CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47172
    Clone ID Related Accession (Same CDS sequence) XM_010806941.2 , XM_010798273.2
    Accession Version XM_010806941.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805243.1
    CDS75..3248
    Translation

    Target ORF information:

    RefSeq Version XM_010806941.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806941.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47172
    Clone ID Related Accession (Same CDS sequence) XM_010806941.2 , XM_010798273.2
    Accession Version XM_010798273.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796575.2
    CDS75..3248
    Translation

    Target ORF information:

    RefSeq Version XM_010798273.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798273.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCAACTTG CCACAACAGT GTTGGAAGCT ACTCTTGTGC CTGCAACTCA
    GGATATATAT CCAGAAGTGG AAAGAAGACT TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CGACTTGCCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCCA GAAGTGGAAA GAAGAATTTC
    CAGGGACCAG GAGAGACATG TGAAGATGTG GACGAATGTT CCAGGAACTC AACTCTTTGT
    GGTCCCAGTT CTGTCTGCAC CAACATCCCA GGAAAATACA GCTGCTCCTG CTTGCCGGGG
    TTCTTTTCAC CTGGTGTGTG GAGCCCTGAA AAACCTGAGG CTTTCAAGTG TGCAGATATT
    GACGAGTGCC TTCAGGATCC CTCACGATGC GGCCCAGATT CTGTCTGCAC CAACACCCTG
    GGCTCCTACA GCTGTGGCTG CGTTGTAGGC TTTCATCCCA ATCCAGAAGG CTCCTGGAAA
    CATGGCAACT TCAGCTGCCA AAGGATTCCC TTCAAGTGTA AAGATGATGT GATACCCAGC
    AACATGCAGG TCCAGTTATG CCATGCGGGA GCAGCGGTGG AGCCTAAATA TGTTTCCTTT
    TGTGCGCTGA TGAATGCCAC CCTCAGCGTT CTGGACAATG TCTGTGAAAA CAAAACCACT
    GTCGTCTCTC TGAAGAGTAC GGCTAAGAGA CTTGACTCTG TGATTGAAAA AACATCCAAG
    TGGTCCAACT TCACCAAAGA AGAGACCTCG ACGCTGGCCA CAGTCTTGCT GGAGAGCGTG
    GAAAGCACCA CGTTGGCAGC TTTTCTGAAA CCCTCAGCGA ATGTCAGTCA GACTATTCAG
    ACGAAACACT TAGATATCGA GAGCAAAGTG ATTGACAAAG AATGCACTAA AGAGAATGAG
    ACATTTAAGT TGAAAGCAAA AGGGGATGAA ATGAAGATTT GGTGTTCCAC AATCAAAGAA
    TCTGAATCCA CAGGGATCAA CGGGGTGGCT TTCGTTTCCT TTTCGGGTAT GGAGTCTATT
    TTGGATGAGC GCTTCTTGAA AGATCTTCAG ATCCCCTGGG CCAGCTCCAA GAAGAAGCTG
    AAGATGAATT CTCGAGTTGT TGGGGGCATC ATAACAGGAG GGAAGAAAGA CGGGTTCTCT
    AAACCAGTAA TCTATACTCT GGAAAACATT AAGCCAAAGC AGGATTTTGA GAGTGCTATC
    TGCGTTTCCT GGAAACCGGA CGTGGAGGGA GGCAGATGGA CACCATCAGG CTGTGTGCTC
    CTTGAAGCTT CCAAGACACA TACTGTCTGC GGCTGTAACC AAATGGTGAA CTTGGCTGTC
    ATCATGGCTT CTGGGGAGGT CACGATGGAG TTCACCCTGT ACCTCATTAG TCACGTGGGC
    ACGATTATCT CACTGGTGTG TCTTGCCATG GCCATCATCA CCTTCCTTCT GTGCCGCACC
    CTCCGAAACC AGAACACCTA CATCCACCTG CATCTCTGCA TATGCCTCTT CTTGGCCAAG
    ATGCTGTTTC TCACTGGTGT GGACAAGACC GACAACCAGA TGGGCTGCGC CCTCATCGCA
    GGCTTTCTGC ACTACCTTTT CCTTGCGTGC TTCTTCTGGA TGCTGGTGGA GGCTGTGATG
    CTCTTCCTCA TGGTCAGGAA CCTGAAAGTG GTGAATTACT TCAGTTCTCG AAACATCAAG
    ATGCTGTATC TCTGCGCCTT TGGCTATGGG CTCCCGGGGC TGGTGGTGGC TGTTTCTGCT
    GGCTTACAAC CACAGGGTTA TGGGATGTAT AATCGCTGCT GGCTGAATAC AGAAACGGGG
    TTCATCTGGA GCTTCCTGGG GCCAGTGTGC ACAATTATAG TGGTCAATTC CATCCTCCTG
    ACTTGGACAC TATGTATCCT GAGGCAGAAG CTTTCTAGTG TCAATGCAGA AGTCTCCACA
    CTCAAAGATA CCAGGTTGCT GACCTTCAAA GCCTTTGCCC AGATCTTCAT CTTGGGGTGC
    TCCTGGGTGT TAGGCATTTT CCAAATTGGA CCCATGGCCA ATATCATGGC TTATCTGTTC
    ACCATCATCA ACAGCTTGCA GGGGGCCTTC ATCTTCCTCA TTCATTGTGT GCTCAGCCGC
    CAGGTACGGG AAGAATACAG AAGATGCACG ACCAGGAAGA CCAAACCCAG CTCTGAGACC
    CAGACCTCAG GGATCTTACT GTCATCTGTT CCCTCCACTT CCAAAACGGG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90255
    Clone ID Related Accession (Same CDS sequence) XM_015464106.1 , XM_015472057.1
    Accession Version XM_015464106.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGAACAGC CACGGTGGTC ATCGTCGAGT 
    CTTGATGGGA ATGGGTGTAC AGATACCACC ATGTGTCCAG CGTATGCAAC CTGCACCAAC
    ACTTCAAGAA GTTACTACTG CTCTTGCAAA AGAGGCTTTC TGTCCAGCAA TGGATTGAAG
    ACATTTAAGG GCCCAGGAGT GGAATGCAAA GATATAGATG AATGCTCTCA AAGTCCCACA
    CCATGTGGCC CTAACTCAAT CTGCAGAAAT CTGCCTGGGA GATATAGGTG CAGCTGTATG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAC TGGATCTCAG GAAAGCAAGG TCATTTCACC
    TGTAGAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGCCAC
    AACAGTCCTG GAAGTTACTC TTGTGTCTGC AACCCTGGTT TTCAATCCAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCTAAA AGAGATGTGT GAAGATGTGG ATGAATGTGC CAGTCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGCCTGCAAC
    CCAGGATATA ACTCCAGAAG TGGAAAGAAG ACTTTCCAGG GACCAGGGGA GACATGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGACC ATTCGACTTG CCACAACAGT
    CTTGGAAACT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGACTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GATGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGACGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGAGTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GACGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319592.1
    CDS16..4275
    Translation

    Target ORF information:

    RefSeq Version XM_015464106.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGAACAGC CACGGTGGTC ATCGTCGAGT 
    CTTGATGGGA ATGGGTGTAC AGATACCACC ATGTGTCCAG CGTATGCAAC CTGCACCAAC
    ACTTCAAGAA GTTACTACTG CTCTTGCAAA AGAGGCTTTC TGTCCAGCAA TGGATTGAAG
    ACATTTAAGG GCCCAGGAGT GGAATGCAAA GATATAGATG AATGCTCTCA AAGTCCCACA
    CCATGTGGCC CTAACTCAAT CTGCAGAAAT CTGCCTGGGA GATATAGGTG CAGCTGTATG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAC TGGATCTCAG GAAAGCAAGG TCATTTCACC
    TGTAGAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGCCAC
    AACAGTCCTG GAAGTTACTC TTGTGTCTGC AACCCTGGTT TTCAATCCAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCTAAA AGAGATGTGT GAAGATGTGG ATGAATGTGC CAGTCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGCCTGCAAC
    CCAGGATATA ACTCCAGAAG TGGAAAGAAG ACTTTCCAGG GACCAGGGGA GACATGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGACC ATTCGACTTG CCACAACAGT
    CTTGGAAACT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGACTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GATGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGACGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGAGTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GACGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90255
    Clone ID Related Accession (Same CDS sequence) XM_015464106.1 , XM_015472057.1
    Accession Version XM_015472057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGAACAGC CACGGTGGTC ATCGTCGAGT 
    CTTGATGGGA ATGGGTGTAC AGATACCACC ATGTGTCCAG CGTATGCAAC CTGCACCAAC
    ACTTCAAGAA GTTACTACTG CTCTTGCAAA AGAGGCTTTC TGTCCAGCAA TGGATTGAAG
    ACATTTAAGG GCCCAGGAGT GGAATGCAAA GATATAGATG AATGCTCTCA AAGTCCCACA
    CCATGTGGCC CTAACTCAAT CTGCAGAAAT CTGCCTGGGA GATATAGGTG CAGCTGTATG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAC TGGATCTCAG GAAAGCAAGG TCATTTCACC
    TGTAGAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGCCAC
    AACAGTCCTG GAAGTTACTC TTGTGTCTGC AACCCTGGTT TTCAATCCAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCTAAA AGAGATGTGT GAAGATGTGG ATGAATGTGC CAGTCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGCCTGCAAC
    CCAGGATATA ACTCCAGAAG TGGAAAGAAG ACTTTCCAGG GACCAGGGGA GACATGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGACC ATTCGACTTG CCACAACAGT
    CTTGGAAACT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGACTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GATGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGACGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGAGTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GACGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327543.1
    CDS16..4275
    Translation

    Target ORF information:

    RefSeq Version XM_015472057.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGAACAGC CACGGTGGTC ATCGTCGAGT 
    CTTGATGGGA ATGGGTGTAC AGATACCACC ATGTGTCCAG CGTATGCAAC CTGCACCAAC
    ACTTCAAGAA GTTACTACTG CTCTTGCAAA AGAGGCTTTC TGTCCAGCAA TGGATTGAAG
    ACATTTAAGG GCCCAGGAGT GGAATGCAAA GATATAGATG AATGCTCTCA AAGTCCCACA
    CCATGTGGCC CTAACTCAAT CTGCAGAAAT CTGCCTGGGA GATATAGGTG CAGCTGTATG
    ACTGGTTTCT CTTCTCCCAC TGGAAATAAC TGGATCTCAG GAAAGCAAGG TCATTTCACC
    TGTAGAGATG TGGACGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGCCAC
    AACAGTCCTG GAAGTTACTC TTGTGTCTGC AACCCTGGTT TTCAATCCAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCTAAA AGAGATGTGT GAAGATGTGG ATGAATGTGC CAGTCCCAGA
    AGTTGCCCAG AGCATTCGAC TTGCCACAAC AGTCTTGGAA GCTACTCTTG TGCCTGCAAC
    CCAGGATATA ACTCCAGAAG TGGAAAGAAG ACTTTCCAGG GACCAGGGGA GACATGTCAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGACC ATTCGACTTG CCACAACAGT
    CTTGGAAACT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CAACTTGCCA CAACAGTGTT GGAAGCTACT CTTGTGCCTG CAACTCAGGA
    TATATATCCA GAAGTGGAAA GAAGACTTTC CAGGGACCAG GGGAGACATG TCAAGATATA
    GATGAATGTT CTCAAAAGCC CCCACCTTGT GGTCCTAACT CAAGGTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCCTCTC CCACGGGAAA TAACTGGATC
    TCTGGAAAGC CAGGTCATTT CACCTGTACA GACATTAATG AATGCCTCTC CCATGGGGTC
    TGCCCTGAGC ATTCTGAGTG CACCAACTCT TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT
    GGATTCACCT CTGGAAACTC CACCTGTGAA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCCACTTG TCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CGAGAAGTGG AAGGAAAAGT TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT
    GTGGACGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CAACTTGCCA CAACAGTGTT
    GGAAGCTACT CTTGTGCCTG CAACTCAGGA TATATATCCA GAAGTGGAAA GAAGAGTTTC
    CAGGGACCAG GGGAGACATG TCAAGATATA GACGAATGTT CTCAAAAGCC CCCACCTTGT
    GGTCCTAACT CAAGGTGCAG AAACCTGCCA GGAAGGTATG AGTGCAGCTG TTTGACTGGT
    TTCTCCTCTC CCACGGGAAA TAACTGGATC TCTGGAAAGC CAGGTCATTT CACCTGTACA
    GACATTAATG AATGCCTCTC CCATGGGGTC TGCCCTGAGC ATTCTGAGTG CACCAACTCT
    TTGGGAAGCT ACCGTTGCAG CTGCAAAGTT GGATTCACCT CTGGAAACTC CACCTGTGAA
    GATGTGGATG AATGTGCCAA TCCCAGAAGT TGCCCAGAGC ATTCCACTTG TCACAACAGT
    CTTGGAAGCT ACTCTTGTGT CTGCAACCCG GGTTTTCAAT CCAGAAGTGG AAAGAAAAGT
    TTCCAGGGGC CAGGAGAGAT GTGTCAAGAT ATAGATGAAT GTTCTCAAAC ACCCCCAACT
    TGTGGTCCTA ACTCAAGCTG CAGAAACCTG CCAGGAAGGT ATGAGTGCAG CTGTTTGACT
    GGTTTCTCTT CTCCCACTGG AAATAATTGG ATCCCTGGAA AGTCAGGTCA TTTTACCTGC
    ACAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG AGCATTCGAC TTGCCACAAC
    AGTCTTGGAA GCTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCCAGAAG TGGAAAGAAG
    AATTTCCAGG GACCAGGAGA GACATGTGAA GATGTGGACG AATGTTCCAG GAACTCAACT
    CTTTGTGGTC CCAGTTCTGT CTGCACCAAC ATCCCAGGAA AATACAGCTG CTCCTGCTTG
    CCGGGGTTCT TTTCACCTGG TGTGTGGAGC CCTGAAAAAC CTGAGGCTTT CAAGTGTGCA
    GATATTGACG AGTGCCTTCA GGATCCCTCA CGATGCGGCC CAGATTCTGT CTGCACCAAC
    ACCCTGGGCT CCTACAGCTG TGGCTGCGTT GTAGGCTTTC ATCCCAATCC AGAAGGCTCC
    TGGAAACATG GCAACTTCAG CTGCCAAAGG ATTCCCTTCA AGTGTAAAGA TGATGTGATA
    CCCAGCAACA TGCAGGTCCA GTTATGCCAT GCGGGAGCAG CGGTGGAGCC TAAATATGTT
    TCCTTTTGTG CGCTGATGAA TGCCACCCTC AGCGTTCTGG ACAATGTCTG TGAAAACAAA
    ACCACTGTCG TCTCTCTGAA GAGTACGGCT AAGAGACTTG ACTCTGTGAT TGAAAAAACA
    TCCAAGTGGT CCAACTTCAC CAAAGAAGAG ACCTCGACGC TGGCCACAGT CTTGCTGGAG
    AGCGTGGAAA GCACCACGTT GGCAGCTTTT CTGAAACCCT CAGCGAATGT CAGTCAGACT
    ATTCAGACGA AACACTTAGA TATCGAGAGC AAAGTGATTG ACAAAGAATG CACTAAAGAG
    AATGAGACAT TTAAGTTGAA AGCAAAAGGG GATGAAATGA AGATTTGGTG TTCCACAATC
    AAAGAATCTG AATCCACAGG GATCAACGGG GTGGCTTTCG TTTCCTTTTC GGGTATGGAG
    TCTATTTTGG ATGAGCGCTT CTTGAAAGAT CTTCAGATCC CCTGGGCCAG CTCCAAGAAG
    AAGCTGAAGA TGAATTCTCG AGTTGTTGGG GGCATCATAA CAGGAGGGAA GAAAGACGGG
    TTCTCTAAAC CAGTAATCTA TACTCTGGAA AACATTAAGC CAAAGCAGGA TTTTGAGAGT
    GCTATCTGCG TTTCCTGGAA ACCGGACGTG GAGGGAGGCA GATGGACACC ATCAGGCTGT
    GTGCTCCTTG AAGCTTCCAA GACACATACT GTCTGCGGCT GTAACCAAAT GGTGAACTTG
    GCTGTCATCA TGGCTTCTGG GGAGGTCACG ATGGAGTTCA CCCTGTACCT CATTAGTCAC
    GTGGGCACGA TTATCTCACT GGTGTGTCTT GCCATGGCCA TCATCACCTT CCTTCTGTGC
    CGCACCCTCC GAAACCAGAA CACCTACATC CACCTGCATC TCTGCATATG CCTCTTCTTG
    GCCAAGATGC TGTTTCTCAC TGGTGTGGAC AAGACCGACA ACCAGATGGG CTGCGCCCTC
    ATCGCAGGCT TTCTGCACTA CCTTTTCCTT GCGTGCTTCT TCTGGATGCT GGTGGAGGCT
    GTGATGCTCT TCCTCATGGT CAGGAACCTG AAAGTGGTGA ATTACTTCAG TTCTCGAAAC
    ATCAAGATGC TGTATCTCTG CGCCTTTGGC TATGGGCTCC CGGGGCTGGT GGTGGCTGTT
    TCTGCTGGCT TACAACCACA GGGTTATGGG ATGTATAATC GCTGCTGGCT GAATACAGAA
    ACGGGGTTCA TCTGGAGCTT CCTGGGGCCA GTGTGCACAA TTATAGTGGT CAATTCCATC
    CTCCTGACTT GGACACTATG TATCCTGAGG CAGAAGCTTT CTAGTGTCAA TGCAGAAGTC
    TCCACACTCA AAGATACCAG GTTGCTGACC TTCAAAGCCT TTGCCCAGAT CTTCATCTTG
    GGGTGCTCCT GGGTGTTAGG CATTTTCCAA ATTGGACCCA TGGCCAATAT CATGGCTTAT
    CTGTTCACCA TCATCAACAG CTTGCAGGGG GCCTTCATCT TCCTCATTCA TTGTGTGCTC
    AGCCGCCAGG TACGGGAAGA ATACAGAAGA TGCACGACCA GGAAGACCAA ACCCAGCTCT
    GAGACCCAGA CCTCAGGGAT CTTACTGTCA TCTGTTCCCT CCACTTCCAA AACGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90256
    Clone ID Related Accession (Same CDS sequence) XM_015464107.1 , XM_015472058.1
    Accession Version XM_015464107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGGGAATG GGTGTACAGA TACCACCATG 
    TGTCCAGCGT ATGCAACCTG CACCAACACT TCAAGAAGTT ACTACTGCTC TTGCAAAAGA
    GGCTTTCTGT CCAGCAATGG ATTGAAGACA TTTAAGGGCC CAGGAGTGGA ATGCAAAGAT
    ATAGATGAAT GCTCTCAAAG TCCCACACCA TGTGGCCCTA ACTCAATCTG CAGAAATCTG
    CCTGGGAGAT ATAGGTGCAG CTGTATGACT GGTTTCTCTT CTCCCACTGG AAATAACTGG
    ATCTCAGGAA AGCAAGGTCA TTTCACCTGT AGAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGCCACAAC AGTCCTGGAA GTTACTCTTG TGTCTGCAAC
    CCTGGTTTTC AATCCAGAAG TGGAAGGAAA AGTTTCCAGG GGCTAAAAGA GATGTGTGAA
    GATGTGGATG AATGTGCCAG TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGC CTGCAACCCA GGATATAACT CCAGAAGTGG AAAGAAGACT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGACCATT CGACTTGCCA CAACAGTCTT GGAAACTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GACTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAT GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319593.1
    CDS36..4262
    Translation

    Target ORF information:

    RefSeq Version XM_015464107.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGGGAATG GGTGTACAGA TACCACCATG 
    TGTCCAGCGT ATGCAACCTG CACCAACACT TCAAGAAGTT ACTACTGCTC TTGCAAAAGA
    GGCTTTCTGT CCAGCAATGG ATTGAAGACA TTTAAGGGCC CAGGAGTGGA ATGCAAAGAT
    ATAGATGAAT GCTCTCAAAG TCCCACACCA TGTGGCCCTA ACTCAATCTG CAGAAATCTG
    CCTGGGAGAT ATAGGTGCAG CTGTATGACT GGTTTCTCTT CTCCCACTGG AAATAACTGG
    ATCTCAGGAA AGCAAGGTCA TTTCACCTGT AGAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGCCACAAC AGTCCTGGAA GTTACTCTTG TGTCTGCAAC
    CCTGGTTTTC AATCCAGAAG TGGAAGGAAA AGTTTCCAGG GGCTAAAAGA GATGTGTGAA
    GATGTGGATG AATGTGCCAG TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGC CTGCAACCCA GGATATAACT CCAGAAGTGG AAAGAAGACT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGACCATT CGACTTGCCA CAACAGTCTT GGAAACTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GACTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAT GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90256
    Clone ID Related Accession (Same CDS sequence) XM_015464107.1 , XM_015472058.1
    Accession Version XM_015472058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGGGAATG GGTGTACAGA TACCACCATG 
    TGTCCAGCGT ATGCAACCTG CACCAACACT TCAAGAAGTT ACTACTGCTC TTGCAAAAGA
    GGCTTTCTGT CCAGCAATGG ATTGAAGACA TTTAAGGGCC CAGGAGTGGA ATGCAAAGAT
    ATAGATGAAT GCTCTCAAAG TCCCACACCA TGTGGCCCTA ACTCAATCTG CAGAAATCTG
    CCTGGGAGAT ATAGGTGCAG CTGTATGACT GGTTTCTCTT CTCCCACTGG AAATAACTGG
    ATCTCAGGAA AGCAAGGTCA TTTCACCTGT AGAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGCCACAAC AGTCCTGGAA GTTACTCTTG TGTCTGCAAC
    CCTGGTTTTC AATCCAGAAG TGGAAGGAAA AGTTTCCAGG GGCTAAAAGA GATGTGTGAA
    GATGTGGATG AATGTGCCAG TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGC CTGCAACCCA GGATATAACT CCAGAAGTGG AAAGAAGACT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGACCATT CGACTTGCCA CAACAGTCTT GGAAACTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GACTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAT GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327544.1
    CDS36..4262
    Translation

    Target ORF information:

    RefSeq Version XM_015472058.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GATGGGAATG GGTGTACAGA TACCACCATG 
    TGTCCAGCGT ATGCAACCTG CACCAACACT TCAAGAAGTT ACTACTGCTC TTGCAAAAGA
    GGCTTTCTGT CCAGCAATGG ATTGAAGACA TTTAAGGGCC CAGGAGTGGA ATGCAAAGAT
    ATAGATGAAT GCTCTCAAAG TCCCACACCA TGTGGCCCTA ACTCAATCTG CAGAAATCTG
    CCTGGGAGAT ATAGGTGCAG CTGTATGACT GGTTTCTCTT CTCCCACTGG AAATAACTGG
    ATCTCAGGAA AGCAAGGTCA TTTCACCTGT AGAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCCAC TTGCCACAAC AGTCCTGGAA GTTACTCTTG TGTCTGCAAC
    CCTGGTTTTC AATCCAGAAG TGGAAGGAAA AGTTTCCAGG GGCTAAAAGA GATGTGTGAA
    GATGTGGATG AATGTGCCAG TCCCAGAAGT TGCCCAGAGC ATTCGACTTG CCACAACAGT
    CTTGGAAGCT ACTCTTGTGC CTGCAACCCA GGATATAACT CCAGAAGTGG AAAGAAGACT
    TTCCAGGGAC CAGGGGAGAC ATGTCAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGACCATT CGACTTGCCA CAACAGTCTT GGAAACTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCGA GAAGTGGAAG GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATGTG
    GATGAATGTG CCAATCCCAG AAGTTGCCCA GAGCATTCAA CTTGCCACAA CAGTGTTGGA
    AGCTACTCTT GTGCCTGCAA CTCAGGATAT ATATCCAGAA GTGGAAAGAA GACTTTCCAG
    GGACCAGGGG AGACATGTCA AGATATAGAT GAATGTTCTC AAAAGCCCCC ACCTTGTGGT
    CCTAACTCAA GGTGCAGAAA CCTGCCAGGA AGGTATGAGT GCAGCTGTTT GACTGGTTTC
    TCCTCTCCCA CGGGAAATAA CTGGATCTCT GGAAAGCCAG GTCATTTCAC CTGTACAGAC
    ATTAATGAAT GCCTCTCCCA TGGGGTCTGC CCTGAGCATT CTGAGTGCAC CAACTCTTTG
    GGAAGCTACC GTTGCAGCTG CAAAGTTGGA TTCACCTCTG GAAACTCCAC CTGTGAAGAT
    GTGGATGAAT GTGCCAATCC CAGAAGTTGC CCAGAGCATT CCACTTGTCA CAACAGTCTT
    GGAAGCTACT CTTGTGTCTG CAACCCGGGT TTTCAATCGA GAAGTGGAAG GAAAAGTTTC
    CAGGGGCCAG GAGAGATGTG TCAAGATGTG GACGAATGTG CCAATCCCAG AAGTTGCCCA
    GAGCATTCAA CTTGCCACAA CAGTGTTGGA AGCTACTCTT GTGCCTGCAA CTCAGGATAT
    ATATCCAGAA GTGGAAAGAA GAGTTTCCAG GGACCAGGGG AGACATGTCA AGATATAGAC
    GAATGTTCTC AAAAGCCCCC ACCTTGTGGT CCTAACTCAA GGTGCAGAAA CCTGCCAGGA
    AGGTATGAGT GCAGCTGTTT GACTGGTTTC TCCTCTCCCA CGGGAAATAA CTGGATCTCT
    GGAAAGCCAG GTCATTTCAC CTGTACAGAC ATTAATGAAT GCCTCTCCCA TGGGGTCTGC
    CCTGAGCATT CTGAGTGCAC CAACTCTTTG GGAAGCTACC GTTGCAGCTG CAAAGTTGGA
    TTCACCTCTG GAAACTCCAC CTGTGAAGAT GTGGATGAAT GTGCCAATCC CAGAAGTTGC
    CCAGAGCATT CCACTTGTCA CAACAGTCTT GGAAGCTACT CTTGTGTCTG CAACCCGGGT
    TTTCAATCCA GAAGTGGAAA GAAAAGTTTC CAGGGGCCAG GAGAGATGTG TCAAGATATA
    GATGAATGTT CTCAAACACC CCCAACTTGT GGTCCTAACT CAAGCTGCAG AAACCTGCCA
    GGAAGGTATG AGTGCAGCTG TTTGACTGGT TTCTCTTCTC CCACTGGAAA TAATTGGATC
    CCTGGAAAGT CAGGTCATTT TACCTGCACA GATGTGGATG AATGTGCCAA TCCCAGAAGT
    TGCCCAGAGC ATTCGACTTG CCACAACAGT CTTGGAAGCT ACTCTTGTGT CTGCAACCCG
    GGTTTTCAAT CCAGAAGTGG AAAGAAGAAT TTCCAGGGAC CAGGAGAGAC ATGTGAAGAT
    GTGGACGAAT GTTCCAGGAA CTCAACTCTT TGTGGTCCCA GTTCTGTCTG CACCAACATC
    CCAGGAAAAT ACAGCTGCTC CTGCTTGCCG GGGTTCTTTT CACCTGGTGT GTGGAGCCCT
    GAAAAACCTG AGGCTTTCAA GTGTGCAGAT ATTGACGAGT GCCTTCAGGA TCCCTCACGA
    TGCGGCCCAG ATTCTGTCTG CACCAACACC CTGGGCTCCT ACAGCTGTGG CTGCGTTGTA
    GGCTTTCATC CCAATCCAGA AGGCTCCTGG AAACATGGCA ACTTCAGCTG CCAAAGGATT
    CCCTTCAAGT GTAAAGATGA TGTGATACCC AGCAACATGC AGGTCCAGTT ATGCCATGCG
    GGAGCAGCGG TGGAGCCTAA ATATGTTTCC TTTTGTGCGC TGATGAATGC CACCCTCAGC
    GTTCTGGACA ATGTCTGTGA AAACAAAACC ACTGTCGTCT CTCTGAAGAG TACGGCTAAG
    AGACTTGACT CTGTGATTGA AAAAACATCC AAGTGGTCCA ACTTCACCAA AGAAGAGACC
    TCGACGCTGG CCACAGTCTT GCTGGAGAGC GTGGAAAGCA CCACGTTGGC AGCTTTTCTG
    AAACCCTCAG CGAATGTCAG TCAGACTATT CAGACGAAAC ACTTAGATAT CGAGAGCAAA
    GTGATTGACA AAGAATGCAC TAAAGAGAAT GAGACATTTA AGTTGAAAGC AAAAGGGGAT
    GAAATGAAGA TTTGGTGTTC CACAATCAAA GAATCTGAAT CCACAGGGAT CAACGGGGTG
    GCTTTCGTTT CCTTTTCGGG TATGGAGTCT ATTTTGGATG AGCGCTTCTT GAAAGATCTT
    CAGATCCCCT GGGCCAGCTC CAAGAAGAAG CTGAAGATGA ATTCTCGAGT TGTTGGGGGC
    ATCATAACAG GAGGGAAGAA AGACGGGTTC TCTAAACCAG TAATCTATAC TCTGGAAAAC
    ATTAAGCCAA AGCAGGATTT TGAGAGTGCT ATCTGCGTTT CCTGGAAACC GGACGTGGAG
    GGAGGCAGAT GGACACCATC AGGCTGTGTG CTCCTTGAAG CTTCCAAGAC ACATACTGTC
    TGCGGCTGTA ACCAAATGGT GAACTTGGCT GTCATCATGG CTTCTGGGGA GGTCACGATG
    GAGTTCACCC TGTACCTCAT TAGTCACGTG GGCACGATTA TCTCACTGGT GTGTCTTGCC
    ATGGCCATCA TCACCTTCCT TCTGTGCCGC ACCCTCCGAA ACCAGAACAC CTACATCCAC
    CTGCATCTCT GCATATGCCT CTTCTTGGCC AAGATGCTGT TTCTCACTGG TGTGGACAAG
    ACCGACAACC AGATGGGCTG CGCCCTCATC GCAGGCTTTC TGCACTACCT TTTCCTTGCG
    TGCTTCTTCT GGATGCTGGT GGAGGCTGTG ATGCTCTTCC TCATGGTCAG GAACCTGAAA
    GTGGTGAATT ACTTCAGTTC TCGAAACATC AAGATGCTGT ATCTCTGCGC CTTTGGCTAT
    GGGCTCCCGG GGCTGGTGGT GGCTGTTTCT GCTGGCTTAC AACCACAGGG TTATGGGATG
    TATAATCGCT GCTGGCTGAA TACAGAAACG GGGTTCATCT GGAGCTTCCT GGGGCCAGTG
    TGCACAATTA TAGTGGTCAA TTCCATCCTC CTGACTTGGA CACTATGTAT CCTGAGGCAG
    AAGCTTTCTA GTGTCAATGC AGAAGTCTCC ACACTCAAAG ATACCAGGTT GCTGACCTTC
    AAAGCCTTTG CCCAGATCTT CATCTTGGGG TGCTCCTGGG TGTTAGGCAT TTTCCAAATT
    GGACCCATGG CCAATATCAT GGCTTATCTG TTCACCATCA TCAACAGCTT GCAGGGGGCC
    TTCATCTTCC TCATTCATTG TGTGCTCAGC CGCCAGGTAC GGGAAGAATA CAGAAGATGC
    ACGACCAGGA AGACCAAACC CAGCTCTGAG ACCCAGACCT CAGGGATCTT ACTGTCATCT
    GTTCCCTCCA CTTCCAAAAC GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90257
    Clone ID Related Accession (Same CDS sequence) XM_015464108.1 , XM_015472059.1
    Accession Version XM_015464108.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319594.1
    CDS75..4250
    Translation

    Target ORF information:

    RefSeq Version XM_015464108.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90257
    Clone ID Related Accession (Same CDS sequence) XM_015464108.1 , XM_015472059.1
    Accession Version XM_015472059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor E1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104016.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327545.1
    CDS75..4250
    Translation

    Target ORF information:

    RefSeq Version XM_015472059.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor E1 (LOC508459), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAGCT TCAGTTTTCT CCTCTTTTGG GGGTGTTGTG GTATATACAC CTGGGGAATG 
    CTCACATTAC CCACACTGGG ATCAATGACA AGGCATGAAC AGCCACGGTG GTCATCGTCG
    AGTCTTGATG GGAATGGGTG TACAGATACC ACCATGTGTC CAGCGTATGC AACCTGCACC
    AACACTTCAA GAAGTTACTA CTGCTCTTGC AAAAGAGGCT TTCTGTCCAG CAATGGATTG
    AAGACATTTA AGGGCCCAGG AGTGGAATGC AAAGATATAG ATGAATGCTC TCAAAGTCCC
    ACACCATGTG GCCCTAACTC AATCTGCAGA AATCTGCCTG GGAGATATAG GTGCAGCTGT
    ATGACTGGTT TCTCTTCTCC CACTGGAAAT AACTGGATCT CAGGAAAGCA AGGTCATTTC
    ACCTGTAGAG ATGTGGACGA ATGTGCCAAT CCCAGAAGTT GCCCAGAGCA TTCCACTTGC
    CACAACAGTC CTGGAAGTTA CTCTTGTGTC TGCAACCCTG GTTTTCAATC CAGAAGTGGA
    AGGAAAAGTT TCCAGGGGCT AAAAGAGATG TGTGAAGATG TGGATGAATG TGCCAGTCCC
    AGAAGTTGCC CAGAGCATTC GACTTGCCAC AACAGTCTTG GAAGCTACTC TTGTGCCTGC
    AACCCAGGAT ATAACTCCAG AAGTGGAAAG AAGACTTTCC AGGGACCAGG GGAGACATGT
    CAAGATGTGG ATGAATGTGC CAATCCCAGA AGTTGCCCAG ACCATTCGAC TTGCCACAAC
    AGTCTTGGAA ACTACTCTTG TGTCTGCAAC CCGGGTTTTC AATCGAGAAG TGGAAGGAAA
    AGTTTCCAGG GGCCAGGAGA GATGTGTCAA GATATAGATG AATGTTCTCA AAAGCCCCCA
    CCTTGTGGTC CTAACTCAAG GTGCAGAAAC CTGCCAGGAA GGTATGAGTG CAGCTGTTTG
    ACTGGTTTCT CCTCTCCCAC GGGAAATAAC TGGATCTCTG GAAAGCCAGG TCATTTCACC
    TGTACAGACA TTAATGAATG CCTCTCCCAT GGGGTCTGCC CTGAGCATTC TGAGTGCACC
    AACTCTTTGG GAAGCTACCG TTGCAGCTGC AAAGTTGGAT TCACCTCTGG AAACTCCACC
    TGTGAAGATG TGGATGAATG TGCCAATCCC AGAAGTTGCC CAGAGCATTC CACTTGTCAC
    AACAGTCTTG GAAGCTACTC TTGTGTCTGC AACCCGGGTT TTCAATCGAG AAGTGGAAGG
    AAAAGTTTCC AGGGGCCAGG AGAGATGTGT CAAGATGTGG ACGAATGTGC CAATCCCAGA
    AGTTGCCCAG AGCATTCAAC TTGCCACAAC AGTGTTGGAA GCTACTCTTG TGCCTGCAAC
    TCAGGATATA TATCCAGAAG TGGAAAGAAG AGTTTCCAGG GACCAGGGGA GACATGTCAA
    GATATAGACG AATGTTCTCA AAAGCCCCCA CCTTGTGGTC CTAACTCAAG GTGCAGAAAC
    CTGCCAGGAA GGTATGAGTG CAGCTGTTTG ACTGGTTTCT CCTCTCCCAC GGGAAATAAC
    TGGATCTCTG GAAAGCCAGG TCATTTCACC TGTACAGACA TTAATGAATG CCTCTCCCAT
    GGGGTCTGCC CTGAGCATTC TGAGTGCACC AACTCTTTGG GAAGCTACCG TTGCAGCTGC
    AAAGTTGGAT TCACCTCTGG AAACTCCACC TGTGAAGATG TGGATGAATG TGCCAATCCC
    AGAAGTTGCC CAGAGCATTC CACTTGTCAC AACAGTCTTG GAAGCTACTC TTGTGTCTGC
    AACCCGGGTT TTCAATCCAG AAGTGGAAAG AAAAGTTTCC AGGGGCCAGG AGAGATGTGT
    CAAGATATAG ATGAATGTTC TCAAACACCC CCAACTTGTG GTCCTAACTC AAGCTGCAGA
    AACCTGCCAG GAAGGTATGA GTGCAGCTGT TTGACTGGTT TCTCTTCTCC CACTGGAAAT
    AATTGGATCC CTGGAAAGTC AGGTCATTTT ACCTGCACAG ATGTGGATGA ATGTGCCAAT
    CCCAGAAGTT GCCCAGAGCA TTCGACTTGC CACAACAGTC TTGGAAGCTA CTCTTGTGTC
    TGCAACCCGG GTTTTCAATC CAGAAGTGGA AAGAAGAATT TCCAGGGACC AGGAGAGACA
    TGTGAAGATG TGGACGAATG TTCCAGGAAC TCAACTCTTT GTGGTCCCAG TTCTGTCTGC
    ACCAACATCC CAGGAAAATA CAGCTGCTCC TGCTTGCCGG GGTTCTTTTC ACCTGGTGTG
    TGGAGCCCTG AAAAACCTGA GGCTTTCAAG TGTGCAGATA TTGACGAGTG CCTTCAGGAT
    CCCTCACGAT GCGGCCCAGA TTCTGTCTGC ACCAACACCC TGGGCTCCTA CAGCTGTGGC
    TGCGTTGTAG GCTTTCATCC CAATCCAGAA GGCTCCTGGA AACATGGCAA CTTCAGCTGC
    CAAAGGATTC CCTTCAAGTG TAAAGATGAT GTGATACCCA GCAACATGCA GGTCCAGTTA
    TGCCATGCGG GAGCAGCGGT GGAGCCTAAA TATGTTTCCT TTTGTGCGCT GATGAATGCC
    ACCCTCAGCG TTCTGGACAA TGTCTGTGAA AACAAAACCA CTGTCGTCTC TCTGAAGAGT
    ACGGCTAAGA GACTTGACTC TGTGATTGAA AAAACATCCA AGTGGTCCAA CTTCACCAAA
    GAAGAGACCT CGACGCTGGC CACAGTCTTG CTGGAGAGCG TGGAAAGCAC CACGTTGGCA
    GCTTTTCTGA AACCCTCAGC GAATGTCAGT CAGACTATTC AGACGAAACA CTTAGATATC
    GAGAGCAAAG TGATTGACAA AGAATGCACT AAAGAGAATG AGACATTTAA GTTGAAAGCA
    AAAGGGGATG AAATGAAGAT TTGGTGTTCC ACAATCAAAG AATCTGAATC CACAGGGATC
    AACGGGGTGG CTTTCGTTTC CTTTTCGGGT ATGGAGTCTA TTTTGGATGA GCGCTTCTTG
    AAAGATCTTC AGATCCCCTG GGCCAGCTCC AAGAAGAAGC TGAAGATGAA TTCTCGAGTT
    GTTGGGGGCA TCATAACAGG AGGGAAGAAA GACGGGTTCT CTAAACCAGT AATCTATACT
    CTGGAAAACA TTAAGCCAAA GCAGGATTTT GAGAGTGCTA TCTGCGTTTC CTGGAAACCG
    GACGTGGAGG GAGGCAGATG GACACCATCA GGCTGTGTGC TCCTTGAAGC TTCCAAGACA
    CATACTGTCT GCGGCTGTAA CCAAATGGTG AACTTGGCTG TCATCATGGC TTCTGGGGAG
    GTCACGATGG AGTTCACCCT GTACCTCATT AGTCACGTGG GCACGATTAT CTCACTGGTG
    TGTCTTGCCA TGGCCATCAT CACCTTCCTT CTGTGCCGCA CCCTCCGAAA CCAGAACACC
    TACATCCACC TGCATCTCTG CATATGCCTC TTCTTGGCCA AGATGCTGTT TCTCACTGGT
    GTGGACAAGA CCGACAACCA GATGGGCTGC GCCCTCATCG CAGGCTTTCT GCACTACCTT
    TTCCTTGCGT GCTTCTTCTG GATGCTGGTG GAGGCTGTGA TGCTCTTCCT CATGGTCAGG
    AACCTGAAAG TGGTGAATTA CTTCAGTTCT CGAAACATCA AGATGCTGTA TCTCTGCGCC
    TTTGGCTATG GGCTCCCGGG GCTGGTGGTG GCTGTTTCTG CTGGCTTACA ACCACAGGGT
    TATGGGATGT ATAATCGCTG CTGGCTGAAT ACAGAAACGG GGTTCATCTG GAGCTTCCTG
    GGGCCAGTGT GCACAATTAT AGTGGTCAAT TCCATCCTCC TGACTTGGAC ACTATGTATC
    CTGAGGCAGA AGCTTTCTAG TGTCAATGCA GAAGTCTCCA CACTCAAAGA TACCAGGTTG
    CTGACCTTCA AAGCCTTTGC CCAGATCTTC ATCTTGGGGT GCTCCTGGGT GTTAGGCATT
    TTCCAAATTG GACCCATGGC CAATATCATG GCTTATCTGT TCACCATCAT CAACAGCTTG
    CAGGGGGCCT TCATCTTCCT CATTCATTGT GTGCTCAGCC GCCAGGTACG GGAAGAATAC
    AGAAGATGCA CGACCAGGAA GACCAAACCC AGCTCTGAGA CCCAGACCTC AGGGATCTTA
    CTGTCATCTG TTCCCTCCAC TTCCAAAACG GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.