NLRP8 cDNA ORF clone, Bos taurus(cattle)

The following NLRP8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225884 XM_024979487.1
Latest version!
Bos taurus NLR family pyrin domain containing 8 (NLRP8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa46473 XM_010815596.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46473 XM_010828108.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46474 XM_010815592.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46474 XM_010828099.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46474 XM_010828098.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46474 XM_010815593.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46475 XM_010815594.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46475 XM_010828102.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46476 XM_010815595.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46476 XM_010828103.2
Latest version!
Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225884
    Clone ID Related Accession (Same CDS sequence) XM_024979487.1
    Accession Version XM_024979487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAGC ATGTTTTCTC GAGCTTAATA GCTAATGATG AGCTATTTTT TCTATTTTTT
    TTTTTTTTAC TCCCACACAG ACCAGAAGGC AGTGACTTGC GTTCTCTCTG GTGGCAAGAC
    TTCTGCTCTG TGTTTAAGAC ACACGAGAGT TTGGAAGTCC TGACTGTGAG AGACAGTGTG
    ATGGACACAG AGGCCGTGGA GACTCTTGCC GCAGCGCTGA GGCATCCCCA CTGTAACCTC
    CGGAAACTGA TCTTTAAGCG TGTGGGCTCC CTCGTGGTGA GTGAAGGCAT CATCAGAGTT
    TTGGTGGAAA ACCAGTACCT GAGACACTTG GAAATACAAG ACACAGAAGT GGGATGCCAA
    GTGATAGATG CTCTCTGCAA CACCCTGAAA CACCCATGGT GTTTTCTGCA GTTTCTCAGC
    CCCTACTCTG TCCGCTGGTT CTTCATCCTG AAGGTGGTTT GGCGGATAAT TCAGATGCTG
    AATGGATGCG GGTTCACCCA GGGAGGATGG AAGCCCCCTC CCGCGGCTCT CGGGGAGGGA
    CGAGGGCCGA GGAGGATGCG AGGTCGGGTG GCTGGGGGCA CCACACCCCC GGGAGCTCAG
    GGCTGCTGTC GCTGTGACTC CCGCAGGCTG GAAGGTTGCC CGTTTGACCC CAGCAACGGG
    GCTGACCTCA CCAGGAGTCT CAAGAGGAAC ATCCACCTGA AGACCCTGAT GCTGAGACGT
    GGCTCCCTGG AGAGGGGCGA GGAGTGTCCC CCGGTGCTGG CGCCCCAGCT GGAGAGGCTG
    TCGCTGGAGA ATTGTGACCT CACGCCGCTT TCCTGCGAAA GTCTTGCCTT TTCTCTCGTG
    AGTAACCAGA GTCTGACCCA CCTGAGCCTG GCAGAAAACG CCTTGCAGGA TGACGGGGTG
    AAGCAGCTCT GGAACATCTT GCAACACTTT CCGTGTCCTC TGCAGAGGCT GGTGCTGAGG
    AACTGCGCCC TGACCTCCGA GTGCTGTCAA GATATAGCAT CTGCTCTTGA TAAAAATAAA
    ACCCTGAGAA GTCTGGACCT CGGCGCTAAC AGGCTGAGGG ACAGCGGCGT GGTCCTGCTG
    TGTCAGCCCC TGTTGAACCC TGACTCTGGC CTCCAGGTTC TCGAGCTGGA GGAGTGCCAG
    TTCACCTCCA TCTGCTGCCC AGCCCTGACC TCCGTGCTCC TCCACAACCG GACCCTGCGG
    TACCTGGACC TCAGCGGAAA CGGCGTCGGG CTCCAGGGCG CCAAGCTCTT GCAGGACGCC
    GCCCTGAAGA GGGTGCTCCG CCCCGAGGTC GTCCTGTACG TCCCGCCGCT TCGGGTGCGG
    CAGCCACAGG CCTGGGAGGG AAGGCGCCTC GTCGGGAGGG CGACCCTGGG CCCGAGCTGT
    TCCTTCCTGA GTTCCCTCGG GGCTCACCAG CTCACCTTTG GCTGTACCTG CAACCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835255.1
    CDS1..3480
    Translation

    Target ORF information:

    RefSeq Version XM_024979487.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 8 (NLRP8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAGC ATGTTTTCTC GAGCTTAATA GCTAATGATG AGCTATTTTT TCTATTTTTT
    TTTTTTTTAC TCCCACACAG ACCAGAAGGC AGTGACTTGC GTTCTCTCTG GTGGCAAGAC
    TTCTGCTCTG TGTTTAAGAC ACACGAGAGT TTGGAAGTCC TGACTGTGAG AGACAGTGTG
    ATGGACACAG AGGCCGTGGA GACTCTTGCC GCAGCGCTGA GGCATCCCCA CTGTAACCTC
    CGGAAACTGA TCTTTAAGCG TGTGGGCTCC CTCGTGGTGA GTGAAGGCAT CATCAGAGTT
    TTGGTGGAAA ACCAGTACCT GAGACACTTG GAAATACAAG ACACAGAAGT GGGATGCCAA
    GTGATAGATG CTCTCTGCAA CACCCTGAAA CACCCATGGT GTTTTCTGCA GTTTCTCAGC
    CCCTACTCTG TCCGCTGGTT CTTCATCCTG AAGGTGGTTT GGCGGATAAT TCAGATGCTG
    AATGGATGCG GGTTCACCCA GGGAGGATGG AAGCCCCCTC CCGCGGCTCT CGGGGAGGGA
    CGAGGGCCGA GGAGGATGCG AGGTCGGGTG GCTGGGGGCA CCACACCCCC GGGAGCTCAG
    GGCTGCTGTC GCTGTGACTC CCGCAGGCTG GAAGGTTGCC CGTTTGACCC CAGCAACGGG
    GCTGACCTCA CCAGGAGTCT CAAGAGGAAC ATCCACCTGA AGACCCTGAT GCTGAGACGT
    GGCTCCCTGG AGAGGGGCGA GGAGTGTCCC CCGGTGCTGG CGCCCCAGCT GGAGAGGCTG
    TCGCTGGAGA ATTGTGACCT CACGCCGCTT TCCTGCGAAA GTCTTGCCTT TTCTCTCGTG
    AGTAACCAGA GTCTGACCCA CCTGAGCCTG GCAGAAAACG CCTTGCAGGA TGACGGGGTG
    AAGCAGCTCT GGAACATCTT GCAACACTTT CCGTGTCCTC TGCAGAGGCT GGTGCTGAGG
    AACTGCGCCC TGACCTCCGA GTGCTGTCAA GATATAGCAT CTGCTCTTGA TAAAAATAAA
    ACCCTGAGAA GTCTGGACCT CGGCGCTAAC AGGCTGAGGG ACAGCGGCGT GGTCCTGCTG
    TGTCAGCCCC TGTTGAACCC TGACTCTGGC CTCCAGGTTC TCGAGCTGGA GGAGTGCCAG
    TTCACCTCCA TCTGCTGCCC AGCCCTGACC TCCGTGCTCC TCCACAACCG GACCCTGCGG
    TACCTGGACC TCAGCGGAAA CGGCGTCGGG CTCCAGGGCG CCAAGCTCTT GCAGGACGCC
    GCCCTGAAGA GGGTGCTCCG CCCCGAGGTC GTCCTGTACG TCCCGCCGCT TCGGGTGCGG
    CAGCCACAGG CCTGGGAGGG AAGGCGCCTC GTCGGGAGGG CGACCCTGGG CCCGAGCTGT
    TCCTTCCTGA GTTCCCTCGG GGCTCACCAG CTCACCTTTG GCTGTACCTG CAACCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46473
    Clone ID Related Accession (Same CDS sequence) XM_010828108.2 , XM_010815596.2
    Accession Version XM_010828108.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010828108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGAAGA AGAAACAAAG CAATGGAGTG GACATGATGA GAAGACTGGA GGGCCCAGTG
    GTGAACAAGG ACATCCTGAA GATGGTCCAG GACTGGAAAG ATGAGTTCTG TGATCAGGCC
    GCAGGGATGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826410.1
    CDS158..3172
    Translation

    Target ORF information:

    RefSeq Version XM_010828108.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010828108.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGAAGA AGAAACAAAG CAATGGAGTG GACATGATGA GAAGACTGGA GGGCCCAGTG
    GTGAACAAGG ACATCCTGAA GATGGTCCAG GACTGGAAAG ATGAGTTCTG TGATCAGGCC
    GCAGGGATGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46473
    Clone ID Related Accession (Same CDS sequence) XM_010828108.2 , XM_010815596.2
    Accession Version XM_010815596.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104482.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGAAGA AGAAACAAAG CAATGGAGTG GACATGATGA GAAGACTGGA GGGCCCAGTG
    GTGAACAAGG ACATCCTGAA GATGGTCCAG GACTGGAAAG ATGAGTTCTG TGATCAGGCC
    GCAGGGATGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813898.1
    CDS158..3172
    Translation

    Target ORF information:

    RefSeq Version XM_010815596.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815596.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGAAGA AGAAACAAAG CAATGGAGTG GACATGATGA GAAGACTGGA GGGCCCAGTG
    GTGAACAAGG ACATCCTGAA GATGGTCCAG GACTGGAAAG ATGAGTTCTG TGATCAGGCC
    GCAGGGATGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46474
    Clone ID Related Accession (Same CDS sequence) XM_010815593.2 , XM_010815592.2 , XM_010828098.2 , XM_010828099.2
    Accession Version XM_010815593.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104482.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813895.1
    CDS134..3319
    Translation

    Target ORF information:

    RefSeq Version XM_010815593.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815593.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46474
    Clone ID Related Accession (Same CDS sequence) XM_010815593.2 , XM_010815592.2 , XM_010828098.2 , XM_010828099.2
    Accession Version XM_010815592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104482.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813894.1
    CDS158..3343
    Translation

    Target ORF information:

    RefSeq Version XM_010815592.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46474
    Clone ID Related Accession (Same CDS sequence) XM_010815593.2 , XM_010815592.2 , XM_010828098.2 , XM_010828099.2
    Accession Version XM_010828098.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010828098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826400.2
    CDS158..3343
    Translation

    Target ORF information:

    RefSeq Version XM_010828098.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010828098.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46474
    Clone ID Related Accession (Same CDS sequence) XM_010815593.2 , XM_010815592.2 , XM_010828098.2 , XM_010828099.2
    Accession Version XM_010828099.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010828099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826401.2
    CDS134..3319
    Translation

    Target ORF information:

    RefSeq Version XM_010828099.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010828099.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGT CGTCCTGAAG
    AAGAAACAAA GCAATGGAGT GGACATGATG AGAAGACTGG AGGGCCCAGT GGTGAACAAG
    GACATCCTGA AGATGGTCCA GGACTGGAAA GATGAGTTCT GTGATCAGGC CGCAGGGATG
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46475
    Clone ID Related Accession (Same CDS sequence) XM_010828102.2 , XM_010815594.2
    Accession Version XM_010828102.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010828102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGA AGAAGAAACA
    AAGCAATGGA GTGGACATGA TGAGAAGACT GGAGGGCCCA GTGGTGAACA AGGACATCCT
    GAAGATGGTC CAGGACTGGA AAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826404.2
    CDS158..3304
    Translation

    Target ORF information:

    RefSeq Version XM_010828102.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010828102.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGA AGAAGAAACA
    AAGCAATGGA GTGGACATGA TGAGAAGACT GGAGGGCCCA GTGGTGAACA AGGACATCCT
    GAAGATGGTC CAGGACTGGA AAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46475
    Clone ID Related Accession (Same CDS sequence) XM_010828102.2 , XM_010815594.2
    Accession Version XM_010815594.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104482.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGA AGAAGAAACA
    AAGCAATGGA GTGGACATGA TGAGAAGACT GGAGGGCCCA GTGGTGAACA AGGACATCCT
    GAAGATGGTC CAGGACTGGA AAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813896.1
    CDS158..3304
    Translation

    Target ORF information:

    RefSeq Version XM_010815594.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815594.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAAGGTTGC
    CCGTTTGACC CCAGCAACGG GGCTGACCTC ACCAGGAGTC TCAAGAGGAA CATCCACCTG
    AAGACCCTGA TGCTGAGACG TGGCTCCCTG GAGAGGGGCG AGGAGTGTCC CCCGGTGCTG
    GCGCCCCAGC TGGAGAGGCT GTCGCTGGAG AATTGTGACC TCACGCCGCT TTCCTGCGAA
    AGTCTTGCCT TTTCTCTCGT GAGTAACCAG AGTCTGACCC ACCTGAGCCT GGCAGAAAAC
    GCCTTGCAGG ATGACGGGGT GAAGCAGCTC TGGAACATCT TGCAACACTT TCCGTGTCCT
    CTGCAGAGGC TGGTGCTGAG GAACTGCGCC CTGACCTCCG AGTGCTGTCA AGATATAGCA
    TCTGCTCTTG ATAAAAATAA AACCCTGAGA AGTCTGGACC TCGGCGCTAA CAGGCTGAGG
    GACAGCGGCG TGGTCCTGCT GTGTCAGCCC CTGTTGAACC CTGACTCTGG CCTCCAGGTT
    CTCGAGCTGG AGGAGTGCCA GTTCACCTCC ATCTGCTGCC CAGCCCTGAC CTCCGTGCTC
    CTCCACAACC GGACCCTGCG GTACCTGGAC CTCAGCGGAA ACGGCGTCGG GCTCCAGGGC
    GCCAAGCTCT TGCAGGACGC CGCCCTGAAG AGGGTGCTCC GCCCCGAGGA AGAAGAAACA
    AAGCAATGGA GTGGACATGA TGAGAAGACT GGAGGGCCCA GTGGTGAACA AGGACATCCT
    GAAGATGGTC CAGGACTGGA AAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46476
    Clone ID Related Accession (Same CDS sequence) XM_010828103.2 , XM_010815595.2
    Accession Version XM_010828103.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010828103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAGAATTGT
    GACCTCACGC CGCTTTCCTG CGAAAGTCTT GCCTTTTCTC TCGTGAGTAA CCAGAGTCTG
    ACCCACCTGA GCCTGGCAGA AAACGCCTTG CAGGATGACG GGGTGAAGCA GCTCTGGAAC
    ATCTTGCAAC ACTTTCCGTG TCCTCTGCAG AGGCTGGTGC TGAGGAACTG CGCCCTGACC
    TCCGAGTGCT GTCAAGATAT AGCATCTGCT CTTGATAAAA ATAAAACCCT GAGAAGTCTG
    GACCTCGGCG CTAACAGGCT GAGGGACAGC GGCGTGGTCC TGCTGTGTCA GCCCCTGTTG
    AACCCTGACT CTGGCCTCCA GGTTCTCGAG CTGGAGGAGT GCCAGTTCAC CTCCATCTGC
    TGCCCAGCCC TGACCTCCGT GCTCCTCCAC AACCGGACCC TGCGGTACCT GGACCTCAGC
    GGAAACGGCG TCGGGCTCCA GGGCGCCAAG CTCTTGCAGG ACGCCGCCCT GAAGAGGGTG
    CTCCGCCCCG AGGTCGTCCT GAAGAAGAAA CAAAGCAATG GAGTGGACAT GATGAGAAGA
    CTGGAGGGCC CAGTGGTGAA CAAGGACATC CTGAAGATGG TCCAGGACTG GAAAGATGAG
    TTCTGTGATC AGGCCGCAGG GATGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826405.2
    CDS158..3187
    Translation

    Target ORF information:

    RefSeq Version XM_010828103.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010828103.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAGAATTGT
    GACCTCACGC CGCTTTCCTG CGAAAGTCTT GCCTTTTCTC TCGTGAGTAA CCAGAGTCTG
    ACCCACCTGA GCCTGGCAGA AAACGCCTTG CAGGATGACG GGGTGAAGCA GCTCTGGAAC
    ATCTTGCAAC ACTTTCCGTG TCCTCTGCAG AGGCTGGTGC TGAGGAACTG CGCCCTGACC
    TCCGAGTGCT GTCAAGATAT AGCATCTGCT CTTGATAAAA ATAAAACCCT GAGAAGTCTG
    GACCTCGGCG CTAACAGGCT GAGGGACAGC GGCGTGGTCC TGCTGTGTCA GCCCCTGTTG
    AACCCTGACT CTGGCCTCCA GGTTCTCGAG CTGGAGGAGT GCCAGTTCAC CTCCATCTGC
    TGCCCAGCCC TGACCTCCGT GCTCCTCCAC AACCGGACCC TGCGGTACCT GGACCTCAGC
    GGAAACGGCG TCGGGCTCCA GGGCGCCAAG CTCTTGCAGG ACGCCGCCCT GAAGAGGGTG
    CTCCGCCCCG AGGTCGTCCT GAAGAAGAAA CAAAGCAATG GAGTGGACAT GATGAGAAGA
    CTGGAGGGCC CAGTGGTGAA CAAGGACATC CTGAAGATGG TCCAGGACTG GAAAGATGAG
    TTCTGTGATC AGGCCGCAGG GATGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46476
    Clone ID Related Accession (Same CDS sequence) XM_010828103.2 , XM_010815595.2
    Accession Version XM_010815595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104482.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAGAATTGT
    GACCTCACGC CGCTTTCCTG CGAAAGTCTT GCCTTTTCTC TCGTGAGTAA CCAGAGTCTG
    ACCCACCTGA GCCTGGCAGA AAACGCCTTG CAGGATGACG GGGTGAAGCA GCTCTGGAAC
    ATCTTGCAAC ACTTTCCGTG TCCTCTGCAG AGGCTGGTGC TGAGGAACTG CGCCCTGACC
    TCCGAGTGCT GTCAAGATAT AGCATCTGCT CTTGATAAAA ATAAAACCCT GAGAAGTCTG
    GACCTCGGCG CTAACAGGCT GAGGGACAGC GGCGTGGTCC TGCTGTGTCA GCCCCTGTTG
    AACCCTGACT CTGGCCTCCA GGTTCTCGAG CTGGAGGAGT GCCAGTTCAC CTCCATCTGC
    TGCCCAGCCC TGACCTCCGT GCTCCTCCAC AACCGGACCC TGCGGTACCT GGACCTCAGC
    GGAAACGGCG TCGGGCTCCA GGGCGCCAAG CTCTTGCAGG ACGCCGCCCT GAAGAGGGTG
    CTCCGCCCCG AGGTCGTCCT GAAGAAGAAA CAAAGCAATG GAGTGGACAT GATGAGAAGA
    CTGGAGGGCC CAGTGGTGAA CAAGGACATC CTGAAGATGG TCCAGGACTG GAAAGATGAG
    TTCTGTGATC AGGCCGCAGG GATGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813897.1
    CDS158..3187
    Translation

    Target ORF information:

    RefSeq Version XM_010815595.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 8 (NLRP8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCGACA TGAACTTGAG CTCTGACCCC TCCTCCTGCC CGTCCTCCCC TCTGTCCCCC 
    TCTGGTGTCT CCCCTCCCTC GCTGGAGTCC TCCTCCTCCT CCACCCTCCC CTTCAGAAAC
    GGAGTCATGC CTTTCATGCT TTCTGTAAGC GCGGAGCATC TACAAAGGTT CAAGCAGCTT
    TTGGTGGAGG AGAACCCCAG ACCCGGCTGC AGCCCGCTCA CCTGGGACCA GCTGAAGTCA
    GCCCGCTGCG GGGAGCTGGT TCATCTGCTC ACTGAGTACT TCCCGGGACA ACGCGCCTGG
    GAGATGGCTC GTGACATCTT CGCCAAGATG AACCAGACAG AGCTGTGTCT TCAGACGCAG
    AGGGAGCTGA ACGAAATTTT ACCTAACTTG GAACCAGAGG CCTTGAGCCT GAGAAAGAGG
    GAGCTGACTT TGGAGGAAGA TGAGTCTGAT AAAATTCGGG AGTACAAGGC ACGTGTGATG
    AGTGAGCACT CAACCCTGTG GGACAGGACA TCTTGGCCTG GGAACAACGT GGACTTCTTT
    TACCAAGAGC CCTGCAGAGA AGACACACTC CTGCAGTGTC TTCTTCTCCC CAGAAAGCCC
    CAAGGAAGAC AGCCCAAGAC CGTGGTCCTC CAGGGAGACG CCGGCGTCGG GAAAACCACC
    GTGGCCAAAA AAGTGATGCA GCAGTGGGCA GAGAACAAGT TCTACGCCCA CAAGGGCTGG
    CTCGCTTTCT ACCTCCACTG CCAGGAGATG GACCGGCCGG AGGAGCAGAG CTTCTCCGAG
    CTGATCGCCT GCAAGTTGTC CGGGTCTCCA GCTCTCGCGT CTAGGATCCT CTCCCACCCA
    GAACACCTCC TCCTCCTGCT GGATGGCTTC GAGGAACTAA CCTTGACCCT CATAGACAGA
    CGGGAGGACC TGAGTGAAGA CTGGAGCCAG AAGATGCCAG GCTCCGTCCT CCTGACCAGT
    CTGTTGAGCA AAAGGATGCT CCCTGATGCC ACGATCATCG TCTTCCTGAG GTTCAGCTTG
    TGGAAGACCG TGGGCCCCTT TCTGAACAGC CCCTCTCTGA TCACCCTGAT GGGCTTCAGT
    GCGCCTGAGA GATCCAGGTA CTTCAGGTCG TACTTCAAAA ACAGGCGCGA TGCCGACGCA
    GCCTTGCGTT TTGCCATGGG CAACGCGGTC CTCTTCTCCA TGTGCCGAGT CCCCGTCATC
    TGCTGGCTGG TCTGCTCTTG TCTGAAGCAA CAGATGGAGA GAGGAAGCAG CCTGCCGCGG
    GCATTCCCCA ATGCCACGGC CGTGTTCATC CACTACCTCT CCAGCCTGTT GCCAACAAGG
    GTGAGGCATG TCGTCGGCGA GACCCCCCAG GAGCAGTTGA AACGCCTGTG CTCCTTGGCT
    GTGGAGGGCA TGTGGAAGAA CCAGTGGGTG TTCAGTGAGA TGGACCTCAA GCTGGCCAGA
    CTGGACGACA GGGACGTCGA GGTGTTTCTC GGGGTGAGGG TCCTCCGGAG GGCGGTGGCT
    GGCGGGGACC TCATCGCCTT CGCCCACCCG AGCTTCCAGG AGTTCTTTGC CGCCCTGCTG
    TTCGTCCTCT GCTTCCCGCA GAGGCTCAGG AATTTCCAAG TGTTGGATCG CTTCCGCATC
    TTTCAGCTGC TCGCTCATCC CGGGAGAAGG AAAAACCACC TGGCTGGGAT GGCGCTGTTC
    CTGTTTGGGC TGTTGAATGA CACATGCGCC CTTGCTGTGG AGCAGCTGTT TGGGTGCAAG
    GTGTCCCTGG GGAACAAGAG GAAGCTGCTG AAGGTCGCCA CCATGCCGCC CGACGGTGAC
    CCCCTGACCC CGCAGCACGG GCTCCCCCAG CTGTTTTACT GTCTGCACGA GATCCGGGAG
    GAGGTCTTCG TGGGTCAGAT CCTCCACAAC CACCGTAAGG CTCTGCTGGT CATCAGCAAG
    ATCAGAGACC TGCAGGTCTC CGCTTTCTGC CTCAAGTTCT GCCGACAGCT GCGGGAGTTA
    GAAGTGACCA TCTCCTGGGC CGTCGCCAAA GCCACCAGCC TCTCACCAGG CCCTCTTCCT
    TCCCCCCAAC CAGAAGGCAG TGACTTGCGT TCTCTCTGGT GGCAAGACTT CTGCTCTGTG
    TTTAAGACAC ACGAGAGTTT GGAAGTCCTG ACTGTGAGAG ACAGTGTGAT GGACACAGAG
    GCCGTGGAGA CTCTTGCCGC AGCGCTGAGG CATCCCCACT GTAACCTCCG GAAACTGATC
    TTTAAGCGTG TGGGCTCCCT CGTGGTGAGT GAAGGCATCA TCAGAGTTTT GGTGGAAAAC
    CAGTACCTGA GACACTTGCA AATACAAGAC ACAGAAGTGG GATGCCAAGT GATAGATGCT
    CTCTGCAACA CCCTGAAACA CCCATGGTGT TTTCTGCAGT TTCTCAGGCT GGAGAATTGT
    GACCTCACGC CGCTTTCCTG CGAAAGTCTT GCCTTTTCTC TCGTGAGTAA CCAGAGTCTG
    ACCCACCTGA GCCTGGCAGA AAACGCCTTG CAGGATGACG GGGTGAAGCA GCTCTGGAAC
    ATCTTGCAAC ACTTTCCGTG TCCTCTGCAG AGGCTGGTGC TGAGGAACTG CGCCCTGACC
    TCCGAGTGCT GTCAAGATAT AGCATCTGCT CTTGATAAAA ATAAAACCCT GAGAAGTCTG
    GACCTCGGCG CTAACAGGCT GAGGGACAGC GGCGTGGTCC TGCTGTGTCA GCCCCTGTTG
    AACCCTGACT CTGGCCTCCA GGTTCTCGAG CTGGAGGAGT GCCAGTTCAC CTCCATCTGC
    TGCCCAGCCC TGACCTCCGT GCTCCTCCAC AACCGGACCC TGCGGTACCT GGACCTCAGC
    GGAAACGGCG TCGGGCTCCA GGGCGCCAAG CTCTTGCAGG ACGCCGCCCT GAAGAGGGTG
    CTCCGCCCCG AGGTCGTCCT GAAGAAGAAA CAAAGCAATG GAGTGGACAT GATGAGAAGA
    CTGGAGGGCC CAGTGGTGAA CAAGGACATC CTGAAGATGG TCCAGGACTG GAAAGATGAG
    TTCTGTGATC AGGCCGCAGG GATGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.