NPHP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHP1
Entrez Gene ID	505421
Full Name	nephrocystin 1
General protein information	Preferred Names nephrocystin 1 Names nephrocystin-1 nephronophthisis 1 (juvenile)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005212297.4	XP_005212354.1	nephrocystin-1 isoform X2
XM_005212296.3	XP_005212353.1	nephrocystin-1 isoform X1
XM_005212298.3	XP_005212355.1	nephrocystin-1 isoform X3
XM_005212298.4	XP_005212355.1	nephrocystin-1 isoform X3
XM_005212297.3	XP_005212354.1	nephrocystin-1 isoform X2
XM_005212296.4	XP_005212353.1	nephrocystin-1 isoform X1
NM_001105332.1	NP_001098802.1	nephrocystin-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPHP1	XP_003309231.1
	NPHP1	XP_002799426.1
Danio rerio (zebrafish)	nphp1	NP_001070638.1
Bos taurus (cattle)	NPHP1	NP_001098802.1
Rattus norvegicus (Norway rat)	Nphp1	NP_001099976.1
Gallus gallus (chicken)	NPHP1	NP_001186358.1
Canis lupus familiaris (dog)	NPHP1	NP_001182770.1
Xenopus tropicalis (tropical clawed frog)	nphp1	XP_002940386.2
Homo sapiens (human)	NPHP1	NP_001121650.1
Mus musculus (house mouse)	Nphp1	NP_058598.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NPHP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa23678	XM_005212298.3 Latest version!	Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa39117	NM_001105332.1 Latest version!	Bos taurus nephrocystin 1 (NPHP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa23676	XM_005212296.3 Latest version!	Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa23677	XM_005212297.3 Latest version!	Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa220182	XM_005212296.4 Latest version!	Bos taurus nephrocystin 1 (NPHP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220183	XM_005212297.4 Latest version!	Bos taurus nephrocystin 1 (NPHP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220184	XM_005212298.4 Latest version!	Bos taurus nephrocystin 1 (NPHP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHP1 ( XM_005212298.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212355.1 Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa23678
Clone ID Related Accession (Same CDS sequence)	XM_005212298.3
Accession Version	XM_005212298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAAA GATGTATCCA GTTAAAACAG GCAATAGATG AAAATAAAAA TGCTCTTCAA 
AAATTAAAAA AAGCTGATGA ACCTGCACCT GTGGGCAATT ATTATCAGAA GAAAGAAGAG 
GAGCACAATC TTTTGGATAA GCTTACCCAC CAACTGCAGG AGCTTGCTGT GTCCATAAGC 
AGAGAAAATG TCCCTGAAAC TGGGGCACTT GCTGAAAGAG AGGAAGAGGA GAACTCTGAT 
GAAGAAGATG AGGAAGAAGA CGACGACGAA GAAGAAAGTG AAGAAAGTGG GGGTGAGGAC 
GAAGAAACAG AAGAGGAAGA GGAAGAGAAA CAGGAAGGTG AATCTCAGCC AGAGGCCACG 
AGTGGAGAAT ACATTGCTCT TGGAGATTTT ACGGCTCAGC AAGTGGGAGA CCTGACATTT 
AAAAAAGGGG AAATTCTCTG TATAATCAAA AAAAACTCTG ATGGTTGGTG GATGGCTAAG 
AACGCCAAAG GAAACGAAGG CCTTATTCCT AAAACCTACT TGGAGCCCTA TAATAAAGAA 
GGAGGTCAAG AATCCAGTGA AGGAGGCAGT GAAGAAGATA CAGAGGTAGG GGATGGAACA 
GTGGATGGAG CCGAAGTCAA GCAAAGAACT GATTCTCACT GGAGTGCTGT CCGGAAGGCC 
ATCTCAGAGC AGATAAACAC CGTCGACGTG CTGACCGCGA TGGGAGCGAT TCCTGCCGGG 
TTCAGGCCTT CCACGCTCTT CCAGCTCCTG GAGGAAGGGA ATCAATTCCG AGCAAGTTAC 
TTCTTACAGC CAGAGCTCAC ACCTTCCCAA CTGGCCTTCA GAGATCTGAT GTGGGATGCA 
AAAACCGGCT CTATCAGGTC AAGACCGAGC CGTGTCTCCT TGATTCTGAC TCTCTGGAGC 
TGCAAGATGA TTCCTCTTCC AGGGGTGAGC ATCCAAGTTC TGAGCAGACA CGTGCGCCTC 
TGTCTGTTTG ACGGCAGTGA GGTTCTGAGC AACATTCACA CAGTCAGGGC CACGTGGCAA 
CCTAAGAAGC CCAAAACATG GACCTTTTCT CCCCAGGTTA CTGGCATCTT GCCCTGCCTG 
CTTGATGGCG ACTGTTTCAT CAGATCCAAC TCTTCCTCTC CAGATCTTGG AATATTATTT 
GAACTTGGAA TTTCTTATAT TCGCAATTCC ACTGGTGAAA GAGGAGAGTT GAGCTGTGGC 
TGGGTGTTTC TGAAACTTTT TGATGCCACT GGAATTCCTA TTCCAGCAAA GACTTACAAG 
CTCTTCTTGA ACGGAGGCAC CCCTTATGAA AAAGGTGTTG AAGTGGACCC TTCAGTATCC 
AGAAGAGCAC ATGGTAGTGT TTTCCATCAG TTGATGACAA TGAGGAGGCA GCCTCAACTT 
CTAGTCAAAC TGAGATCTTT GAACAGAAGA TCCAGGAGCA CACTAAGTCT ACTACCAGAA 
ACATTAATCG GAAGCATGTG CTGCGTTCAT TTGCTGATAT TCTATCGCCA GATTCTTGGA 
GATGTTCTCC TGAAAGACAG GATGAGCCTG AACAGTGCAG ACTTAATTAG TAATCCAGTG 
CTGGCCACCT TCCCCAAGCT CTTGGAGGAG TCGGACATAA TGGATGCACT CAGGAGTTCC 
TGGGCTGAGA AAGAGAGTAC ATTAAAAAGA TCAGAGAAGG GAGACAGGGA GCTCCTGAAG 
GCGGTGTTCC TGCTGGTTTA CCACGACTGC GCGCTCCCCC TGCTGCACTC TGCGCTCCTG 
CCCCCGTCTA GATGGGCAGA GGAGGAGACT GAGGCTGCCC GGTGGAAGGG CATCGCCGAC 
TTCCTCAAGC AAAACCAGGA AAATGAGGGC GCCCTCCAGG TTCTGCTGTC GCCAGAGGGG 
GTCCACGAAC CTTTCGACAT TTCCGAGCAG ACCTATGACT TCTTGGGTGA AAGAAGAAAG 
AACGTAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212355.1
CDS	187..2118
Translation	MYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	XM_005212298.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212298.3

ORF Insert Sequence:

ATGTACCAAA GATGTATCCA GTTAAAACAG GCAATAGATG AAAATAAAAA TGCTCTTCAA 
AAATTAAAAA AAGCTGATGA ACCTGCACCT GTGGGCAATT ATTATCAGAA GAAAGAAGAG 
GAGCACAATC TTTTGGATAA GCTTACCCAC CAACTGCAGG AGCTTGCTGT GTCCATAAGC 
AGAGAAAATG TCCCTGAAAC TGGGGCACTT GCTGAAAGAG AGGAAGAGGA GAACTCTGAT 
GAAGAAGATG AGGAAGAAGA CGACGACGAA GAAGAAAGTG AAGAAAGTGG GGGTGAGGAC 
GAAGAAACAG AAGAGGAAGA GGAAGAGAAA CAGGAAGGTG AATCTCAGCC AGAGGCCACG 
AGTGGAGAAT ACATTGCTCT TGGAGATTTT ACGGCTCAGC AAGTGGGAGA CCTGACATTT 
AAAAAAGGGG AAATTCTCTG TATAATCAAA AAAAACTCTG ATGGTTGGTG GATGGCTAAG 
AACGCCAAAG GAAACGAAGG CCTTATTCCT AAAACCTACT TGGAGCCCTA TAATAAAGAA 
GGAGGTCAAG AATCCAGTGA AGGAGGCAGT GAAGAAGATA CAGAGGTAGG GGATGGAACA 
GTGGATGGAG CCGAAGTCAA GCAAAGAACT GATTCTCACT GGAGTGCTGT CCGGAAGGCC 
ATCTCAGAGC AGATAAACAC CGTCGACGTG CTGACCGCGA TGGGAGCGAT TCCTGCCGGG 
TTCAGGCCTT CCACGCTCTT CCAGCTCCTG GAGGAAGGGA ATCAATTCCG AGCAAGTTAC 
TTCTTACAGC CAGAGCTCAC ACCTTCCCAA CTGGCCTTCA GAGATCTGAT GTGGGATGCA 
AAAACCGGCT CTATCAGGTC AAGACCGAGC CGTGTCTCCT TGATTCTGAC TCTCTGGAGC 
TGCAAGATGA TTCCTCTTCC AGGGGTGAGC ATCCAAGTTC TGAGCAGACA CGTGCGCCTC 
TGTCTGTTTG ACGGCAGTGA GGTTCTGAGC AACATTCACA CAGTCAGGGC CACGTGGCAA 
CCTAAGAAGC CCAAAACATG GACCTTTTCT CCCCAGGTTA CTGGCATCTT GCCCTGCCTG 
CTTGATGGCG ACTGTTTCAT CAGATCCAAC TCTTCCTCTC CAGATCTTGG AATATTATTT 
GAACTTGGAA TTTCTTATAT TCGCAATTCC ACTGGTGAAA GAGGAGAGTT GAGCTGTGGC 
TGGGTGTTTC TGAAACTTTT TGATGCCACT GGAATTCCTA TTCCAGCAAA GACTTACAAG 
CTCTTCTTGA ACGGAGGCAC CCCTTATGAA AAAGGTGTTG AAGTGGACCC TTCAGTATCC 
AGAAGAGCAC ATGGTAGTGT TTTCCATCAG TTGATGACAA TGAGGAGGCA GCCTCAACTT 
CTAGTCAAAC TGAGATCTTT GAACAGAAGA TCCAGGAGCA CACTAAGTCT ACTACCAGAA 
ACATTAATCG GAAGCATGTG CTGCGTTCAT TTGCTGATAT TCTATCGCCA GATTCTTGGA 
GATGTTCTCC TGAAAGACAG GATGAGCCTG AACAGTGCAG ACTTAATTAG TAATCCAGTG 
CTGGCCACCT TCCCCAAGCT CTTGGAGGAG TCGGACATAA TGGATGCACT CAGGAGTTCC 
TGGGCTGAGA AAGAGAGTAC ATTAAAAAGA TCAGAGAAGG GAGACAGGGA GCTCCTGAAG 
GCGGTGTTCC TGCTGGTTTA CCACGACTGC GCGCTCCCCC TGCTGCACTC TGCGCTCCTG 
CCCCCGTCTA GATGGGCAGA GGAGGAGACT GAGGCTGCCC GGTGGAAGGG CATCGCCGAC 
TTCCTCAAGC AAAACCAGGA AAATGAGGGC GCCCTCCAGG TTCTGCTGTC GCCAGAGGGG 
GTCCACGAAC CTTTCGACAT TTCCGAGCAG ACCTATGACT TCTTGGGTGA AAGAAGAAAG 
AACGTAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( NM_001105332.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001098802.1 Bos taurus nephrocystin 1 (NPHP1), mRNA.

Price & Availability↑

CloneID	OBa39117
Clone ID Related Accession (Same CDS sequence)	NM_001105332.1
Accession Version	NM_001105332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	nephrocystin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134536.1. On Sep 25, 2007 this sequence version replaced XM_581708.3. ##Evidence-Data-START## Transcript exon combination :: BC134536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GATAAACACC GTCGACGTGC TGACCGCGAT GGGAGCGATT 
CCTGCCGGGT TCAGGCCTTC CACGCTCTTC CAGCTCCTGG AGGAAGGGAA TCAATTCCGA 
GCAAGTTACT TCTTACAGCC AGAGCTCACA CCTTCCCAAC TGGCCTTCAG AGATCTGATG 
TGGGATGCAA AAACCGGCTC TATCAGGTCA AGACCGAGCC GTGTCTCCTT GATTCTGACT 
CTCTGGAGCT GCAAGATGAT TCCTCTTCCA GGGGTGAGCA TCCAAGTTCT GAGCAGACAC 
GTGCGCCTCT GTCTGTTTGA CGGCAGTGAG GTTCTGAGCA ACATTCACAC AGTCAGGGCC 
ACGTGGCAAC CTAAGAAGCC CAAAACATGG ACCTTTTCTC CCCAGGTTAC TGGCATCTTG 
CCCTGCCTGC TTGATGGCGA CTGTTTCATC AGATCCAACT CTTCCTCTCC AGATCTTGGA 
ATATTATTTG AACTTGGAAT TTCTTATATT CGCAATTCCA CTGGTGAAAG AGGAGAGTTG 
AGCTGTGGCT GGGTGTTTCT GAAACTTTTT GATGCCACTG GAATTCCTAT TCCAGCAAAG 
ACTTACAAGC TCTTCTTGAA CGGAGGCACC CCTTATGAAA AAGGTGTTGA AGTGGACCCT 
TCAGTATCCA GAAGAGCACA TGGTAGTGTT TTCCATCAGT TGATGACAAT GAGGAGGCAG 
CCTCAACTTC TAGTCAAACT GAGATCTTTG AACAGAAGAT CCAGGAGCAC ACTAAGTCTA 
CTACCAGAAA CATTAATCGG AAGCATGTGC TGCGTTCATT TGCTGATATT CTATCGCCAG 
ATTCTTGGAG ATGTTCTCCT GAAAGACAGG ATGAGCCTGA ACAGTGCAGA CTTAATTAGT 
AATCCAGTGC TGGCCACCTT CCCCAAGCTC TTGGAGGAGT CGGACATAAT GGATGCACTC 
AGGAGTTCCT GGGCTGAGAA AGAGAGTACA TTAAAAAGAT CAGAGAAGGG AGACAGGGAG 
CTCCTGAAGG CGGTGTTCCT GCTGGTTTAC CACGACTGCG CGCTCCCCCT GCTGCACTCT 
GCGCTCCTGC CCCCGTCTAG ATGGGCAGAG GAGGAGACTG AGGCTGCCCG GTGGAAGGGC 
ATCGCCGACT TCCTTAAGCA AAACCAGGAA AATGAGGGCG CCCTCCAGGT TCTGCTGTCG 
CCAGAGGGGG TCCACGAACC TTTCGACATT TCCGAGCAGA CCTATGACTT CTTGGGTGAA 
AGAAGAAAGA ACGTAACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098802.1
CDS	27..2087
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	NM_001105332.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 1 (NPHP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105332.1

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GATAAACACC GTCGACGTGC TGACCGCGAT GGGAGCGATT 
CCTGCCGGGT TCAGGCCTTC CACGCTCTTC CAGCTCCTGG AGGAAGGGAA TCAATTCCGA 
GCAAGTTACT TCTTACAGCC AGAGCTCACA CCTTCCCAAC TGGCCTTCAG AGATCTGATG 
TGGGATGCAA AAACCGGCTC TATCAGGTCA AGACCGAGCC GTGTCTCCTT GATTCTGACT 
CTCTGGAGCT GCAAGATGAT TCCTCTTCCA GGGGTGAGCA TCCAAGTTCT GAGCAGACAC 
GTGCGCCTCT GTCTGTTTGA CGGCAGTGAG GTTCTGAGCA ACATTCACAC AGTCAGGGCC 
ACGTGGCAAC CTAAGAAGCC CAAAACATGG ACCTTTTCTC CCCAGGTTAC TGGCATCTTG 
CCCTGCCTGC TTGATGGCGA CTGTTTCATC AGATCCAACT CTTCCTCTCC AGATCTTGGA 
ATATTATTTG AACTTGGAAT TTCTTATATT CGCAATTCCA CTGGTGAAAG AGGAGAGTTG 
AGCTGTGGCT GGGTGTTTCT GAAACTTTTT GATGCCACTG GAATTCCTAT TCCAGCAAAG 
ACTTACAAGC TCTTCTTGAA CGGAGGCACC CCTTATGAAA AAGGTGTTGA AGTGGACCCT 
TCAGTATCCA GAAGAGCACA TGGTAGTGTT TTCCATCAGT TGATGACAAT GAGGAGGCAG 
CCTCAACTTC TAGTCAAACT GAGATCTTTG AACAGAAGAT CCAGGAGCAC ACTAAGTCTA 
CTACCAGAAA CATTAATCGG AAGCATGTGC TGCGTTCATT TGCTGATATT CTATCGCCAG 
ATTCTTGGAG ATGTTCTCCT GAAAGACAGG ATGAGCCTGA ACAGTGCAGA CTTAATTAGT 
AATCCAGTGC TGGCCACCTT CCCCAAGCTC TTGGAGGAGT CGGACATAAT GGATGCACTC 
AGGAGTTCCT GGGCTGAGAA AGAGAGTACA TTAAAAAGAT CAGAGAAGGG AGACAGGGAG 
CTCCTGAAGG CGGTGTTCCT GCTGGTTTAC CACGACTGCG CGCTCCCCCT GCTGCACTCT 
GCGCTCCTGC CCCCGTCTAG ATGGGCAGAG GAGGAGACTG AGGCTGCCCG GTGGAAGGGC 
ATCGCCGACT TCCTTAAGCA AAACCAGGAA AATGAGGGCG CCCTCCAGGT TCTGCTGTCG 
CCAGAGGGGG TCCACGAACC TTTCGACATT TCCGAGCAGA CCTATGACTT CTTGGGTGAA 
AGAAGAAAGA ACGTAACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_005212296.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212353.1 Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23676
Clone ID Related Accession (Same CDS sequence)	XM_005212296.3
Accession Version	XM_005212296.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTGA GCATCCAAGT TCTGAGCAGA 
CACGTGCGCC TCTGTCTGTT TGACGGCAGT GAGGTTCTGA GCAACATTCA CACAGTCAGG 
GCCACGTGGC AACCTAAGAA GCCCAAAACA TGGACCTTTT CTCCCCAGGT TACTGGCATC 
TTGCCCTGCC TGCTTGATGG CGACTGTTTC ATCAGATCCA ACTCTTCCTC TCCAGATCTT 
GGAATATTAT TTGAACTTGG AATTTCTTAT ATTCGCAATT CCACTGGTGA AAGAGGAGAG 
TTGAGCTGTG GCTGGGTGTT TCTGAAACTT TTTGATGCCA CTGGAATTCC TATTCCAGCA 
AAGACTTACA AGCTCTTCTT GAACGGAGGC ACCCCTTATG AAAAAGGTGT TGAAGTGGAC 
CCTTCAGTAT CCAGAAGAGC ACATGGTAGT GTTTTCCATC AGTTGATGAC AATGAGGAGG 
CAGCCTCAAC TTCTAGTCAA ACTGAGATCT TTGAACAGAA GATCCAGGAG CACACTAAGT 
CTACTACCAG AAACATTAAT CGGAAGCATG TGCTGCGTTC ATTTGCTGAT ATTCTATCGC 
CAGATTCTTG GAGATGTTCT CCTGAAAGAC AGGATGAGCC TGAACAGTGC AGACTTAATT 
AGTAATCCAG TGCTGGCCAC CTTCCCCAAG CTCTTGGAGG AGTCGGACAT AATGGATGCA 
CTCAGGAGTT CCTGGGCTGA GAAAGAGAGT ACATTAAAAA GATCAGAGAA GGGAGACAGG 
GAGCTCCTGA AGGCGGTGTT CCTGCTGGTT TACCACGACT GCGCGCTCCC CCTGCTGCAC 
TCTGCGCTCC TGCCCCCGTC TAGATGGGCA GAGGAGGAGA CTGAGGCTGC CCGGTGGAAG 
GGCATCGCCG ACTTCCTCAA GCAAAACCAG GAAAATGAGG GCGCCCTCCA GGTTCTGCTG 
TCGCCAGAGG GGGTCCACGA ACCTTTCGAC ATTTCCGAGC AGACCTATGA CTTCTTGGGT 
GAAAGAAGAA AGAACGTAAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212353.1
CDS	253..2316
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	XM_005212296.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212296.3

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTGA GCATCCAAGT TCTGAGCAGA 
CACGTGCGCC TCTGTCTGTT TGACGGCAGT GAGGTTCTGA GCAACATTCA CACAGTCAGG 
GCCACGTGGC AACCTAAGAA GCCCAAAACA TGGACCTTTT CTCCCCAGGT TACTGGCATC 
TTGCCCTGCC TGCTTGATGG CGACTGTTTC ATCAGATCCA ACTCTTCCTC TCCAGATCTT 
GGAATATTAT TTGAACTTGG AATTTCTTAT ATTCGCAATT CCACTGGTGA AAGAGGAGAG 
TTGAGCTGTG GCTGGGTGTT TCTGAAACTT TTTGATGCCA CTGGAATTCC TATTCCAGCA 
AAGACTTACA AGCTCTTCTT GAACGGAGGC ACCCCTTATG AAAAAGGTGT TGAAGTGGAC 
CCTTCAGTAT CCAGAAGAGC ACATGGTAGT GTTTTCCATC AGTTGATGAC AATGAGGAGG 
CAGCCTCAAC TTCTAGTCAA ACTGAGATCT TTGAACAGAA GATCCAGGAG CACACTAAGT 
CTACTACCAG AAACATTAAT CGGAAGCATG TGCTGCGTTC ATTTGCTGAT ATTCTATCGC 
CAGATTCTTG GAGATGTTCT CCTGAAAGAC AGGATGAGCC TGAACAGTGC AGACTTAATT 
AGTAATCCAG TGCTGGCCAC CTTCCCCAAG CTCTTGGAGG AGTCGGACAT AATGGATGCA 
CTCAGGAGTT CCTGGGCTGA GAAAGAGAGT ACATTAAAAA GATCAGAGAA GGGAGACAGG 
GAGCTCCTGA AGGCGGTGTT CCTGCTGGTT TACCACGACT GCGCGCTCCC CCTGCTGCAC 
TCTGCGCTCC TGCCCCCGTC TAGATGGGCA GAGGAGGAGA CTGAGGCTGC CCGGTGGAAG 
GGCATCGCCG ACTTCCTCAA GCAAAACCAG GAAAATGAGG GCGCCCTCCA GGTTCTGCTG 
TCGCCAGAGG GGGTCCACGA ACCTTTCGAC ATTTCCGAGC AGACCTATGA CTTCTTGGGT 
GAAAGAAGAA AGAACGTAAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_005212297.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212354.1 Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23677
Clone ID Related Accession (Same CDS sequence)	XM_005212297.3
Accession Version	XM_005212297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTTC TGAGCAACAT TCACACAGTC 
AGGGCCACGT GGCAACCTAA GAAGCCCAAA ACATGGACCT TTTCTCCCCA GGTTACTGGC 
ATCTTGCCCT GCCTGCTTGA TGGCGACTGT TTCATCAGAT CCAACTCTTC CTCTCCAGAT 
CTTGGAATAT TATTTGAACT TGGAATTTCT TATATTCGCA ATTCCACTGG TGAAAGAGGA 
GAGTTGAGCT GTGGCTGGGT GTTTCTGAAA CTTTTTGATG CCACTGGAAT TCCTATTCCA 
GCAAAGACTT ACAAGCTCTT CTTGAACGGA GGCACCCCTT ATGAAAAAGG TGTTGAAGTG 
GACCCTTCAG TATCCAGAAG AGCACATGGT AGTGTTTTCC ATCAGTTGAT GACAATGAGG 
AGGCAGCCTC AACTTCTAGT CAAACTGAGA TCTTTGAACA GAAGATCCAG GAGCACACTA 
AGTCTACTAC CAGAAACATT AATCGGAAGC ATGTGCTGCG TTCATTTGCT GATATTCTAT 
CGCCAGATTC TTGGAGATGT TCTCCTGAAA GACAGGATGA GCCTGAACAG TGCAGACTTA 
ATTAGTAATC CAGTGCTGGC CACCTTCCCC AAGCTCTTGG AGGAGTCGGA CATAATGGAT 
GCACTCAGGA GTTCCTGGGC TGAGAAAGAG AGTACATTAA AAAGATCAGA GAAGGGAGAC 
AGGGAGCTCC TGAAGGCGGT GTTCCTGCTG GTTTACCACG ACTGCGCGCT CCCCCTGCTG 
CACTCTGCGC TCCTGCCCCC GTCTAGATGG GCAGAGGAGG AGACTGAGGC TGCCCGGTGG 
AAGGGCATCG CCGACTTCCT CAAGCAAAAC CAGGAAAATG AGGGCGCCCT CCAGGTTCTG 
CTGTCGCCAG AGGGGGTCCA CGAACCTTTC GACATTTCCG AGCAGACCTA TGACTTCTTG 
GGTGAAAGAA GAAAGAACGT AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212354.1
CDS	257..2263
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	XM_005212297.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 1 (juvenile) (NPHP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212297.3

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTTC TGAGCAACAT TCACACAGTC 
AGGGCCACGT GGCAACCTAA GAAGCCCAAA ACATGGACCT TTTCTCCCCA GGTTACTGGC 
ATCTTGCCCT GCCTGCTTGA TGGCGACTGT TTCATCAGAT CCAACTCTTC CTCTCCAGAT 
CTTGGAATAT TATTTGAACT TGGAATTTCT TATATTCGCA ATTCCACTGG TGAAAGAGGA 
GAGTTGAGCT GTGGCTGGGT GTTTCTGAAA CTTTTTGATG CCACTGGAAT TCCTATTCCA 
GCAAAGACTT ACAAGCTCTT CTTGAACGGA GGCACCCCTT ATGAAAAAGG TGTTGAAGTG 
GACCCTTCAG TATCCAGAAG AGCACATGGT AGTGTTTTCC ATCAGTTGAT GACAATGAGG 
AGGCAGCCTC AACTTCTAGT CAAACTGAGA TCTTTGAACA GAAGATCCAG GAGCACACTA 
AGTCTACTAC CAGAAACATT AATCGGAAGC ATGTGCTGCG TTCATTTGCT GATATTCTAT 
CGCCAGATTC TTGGAGATGT TCTCCTGAAA GACAGGATGA GCCTGAACAG TGCAGACTTA 
ATTAGTAATC CAGTGCTGGC CACCTTCCCC AAGCTCTTGG AGGAGTCGGA CATAATGGAT 
GCACTCAGGA GTTCCTGGGC TGAGAAAGAG AGTACATTAA AAAGATCAGA GAAGGGAGAC 
AGGGAGCTCC TGAAGGCGGT GTTCCTGCTG GTTTACCACG ACTGCGCGCT CCCCCTGCTG 
CACTCTGCGC TCCTGCCCCC GTCTAGATGG GCAGAGGAGG AGACTGAGGC TGCCCGGTGG 
AAGGGCATCG CCGACTTCCT CAAGCAAAAC CAGGAAAATG AGGGCGCCCT CCAGGTTCTG 
CTGTCGCCAG AGGGGGTCCA CGAACCTTTC GACATTTCCG AGCAGACCTA TGACTTCTTG 
GGTGAAAGAA GAAAGAACGT AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_005212296.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212353.1 Bos taurus nephrocystin 1 (NPHP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220182
Clone ID Related Accession (Same CDS sequence)	XM_005212296.4
Accession Version	XM_005212296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTGA GCATCCAAGT TCTGAGCAGA 
CACGTGCGCC TCTGTCTGTT TGACGGCAGT GAGGTTCTGA GCAACATTCA CACAGTCAGG 
GCCACGTGGC AACCTAAGAA GCCCAAAACA TGGACCTTTT CTCCCCAGGT TACTGGCATC 
TTGCCCTGCC TGCTTGATGG CGACTGTTTC ATCAGATCCA ACTCTTCCTC TCCAGATCTT 
GGAATATTAT TTGAACTTGG AATTTCTTAT ATTCGCAATT CCACTGGTGA AAGAGGAGAG 
TTGAGCTGTG GCTGGGTGTT TCTGAAACTT TTTGATGCCA CTGGAATTCC TATTCCAGCA 
AAGACTTACA AGCTCTTCTT GAACGGAGGC ACCCCTTATG AAAAAGGTGT TGAAGTGGAC 
CCTTCAGTAT CCAGAAGAGC ACATGGTAGT GTTTTCCATC AGTTGATGAC AATGAGGAGG 
CAGCCTCAAC TTCTAGTCAA ACTGAGATCT TTGAACAGAA GATCCAGGAG CACACTAAGT 
CTACTACCAG AAACATTAAT CGGAAGCATG TGCTGCGTTC ATTTGCTGAT ATTCTATCGC 
CAGATTCTTG GAGATGTTCT CCTGAAAGAC AGGATGAGCC TGAACAGTGC AGACTTAATT 
AGTAATCCAG TGCTGGCCAC CTTCCCCAAG CTCTTGGAGG AGTCGGACAT AATGGATGCA 
CTCAGGAGTT CCTGGGCTGA GAAAGAGAGT ACATTAAAAA GATCAGAGAA GGGAGACAGG 
GAGCTCCTGA AGGCGGTGTT CCTGCTGGTT TACCACGACT GCGCGCTCCC CCTGCTGCAC 
TCTGCGCTCC TGCCCCCGTC TAGATGGGCA GAGGAGGAGA CTGAGGCTGC CCGGTGGAAG 
GGCATCGCCG ACTTCCTTAA GCAAAACCAG GAAAATGAGG GCGCCCTCCA GGTTCTGCTG 
TCGCCAGAGG GGGTCCACGA ACCTTTCGAC ATTTCCGAGC AGACCTATGA CTTCTTGGGT 
GAAAGAAGAA AGAACGTAAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212353.1
CDS	607..2670
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	XM_005212296.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 1 (NPHP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212296.4

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTGA GCATCCAAGT TCTGAGCAGA 
CACGTGCGCC TCTGTCTGTT TGACGGCAGT GAGGTTCTGA GCAACATTCA CACAGTCAGG 
GCCACGTGGC AACCTAAGAA GCCCAAAACA TGGACCTTTT CTCCCCAGGT TACTGGCATC 
TTGCCCTGCC TGCTTGATGG CGACTGTTTC ATCAGATCCA ACTCTTCCTC TCCAGATCTT 
GGAATATTAT TTGAACTTGG AATTTCTTAT ATTCGCAATT CCACTGGTGA AAGAGGAGAG 
TTGAGCTGTG GCTGGGTGTT TCTGAAACTT TTTGATGCCA CTGGAATTCC TATTCCAGCA 
AAGACTTACA AGCTCTTCTT GAACGGAGGC ACCCCTTATG AAAAAGGTGT TGAAGTGGAC 
CCTTCAGTAT CCAGAAGAGC ACATGGTAGT GTTTTCCATC AGTTGATGAC AATGAGGAGG 
CAGCCTCAAC TTCTAGTCAA ACTGAGATCT TTGAACAGAA GATCCAGGAG CACACTAAGT 
CTACTACCAG AAACATTAAT CGGAAGCATG TGCTGCGTTC ATTTGCTGAT ATTCTATCGC 
CAGATTCTTG GAGATGTTCT CCTGAAAGAC AGGATGAGCC TGAACAGTGC AGACTTAATT 
AGTAATCCAG TGCTGGCCAC CTTCCCCAAG CTCTTGGAGG AGTCGGACAT AATGGATGCA 
CTCAGGAGTT CCTGGGCTGA GAAAGAGAGT ACATTAAAAA GATCAGAGAA GGGAGACAGG 
GAGCTCCTGA AGGCGGTGTT CCTGCTGGTT TACCACGACT GCGCGCTCCC CCTGCTGCAC 
TCTGCGCTCC TGCCCCCGTC TAGATGGGCA GAGGAGGAGA CTGAGGCTGC CCGGTGGAAG 
GGCATCGCCG ACTTCCTTAA GCAAAACCAG GAAAATGAGG GCGCCCTCCA GGTTCTGCTG 
TCGCCAGAGG GGGTCCACGA ACCTTTCGAC ATTTCCGAGC AGACCTATGA CTTCTTGGGT 
GAAAGAAGAA AGAACGTAAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_005212297.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212354.1 Bos taurus nephrocystin 1 (NPHP1), transcript variant X2, mRNA.

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CloneID	OBa220183
Clone ID Related Accession (Same CDS sequence)	XM_005212297.4
Accession Version	XM_005212297.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212297.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTTC TGAGCAACAT TCACACAGTC 
AGGGCCACGT GGCAACCTAA GAAGCCCAAA ACATGGACCT TTTCTCCCCA GGTTACTGGC 
ATCTTGCCCT GCCTGCTTGA TGGCGACTGT TTCATCAGAT CCAACTCTTC CTCTCCAGAT 
CTTGGAATAT TATTTGAACT TGGAATTTCT TATATTCGCA ATTCCACTGG TGAAAGAGGA 
GAGTTGAGCT GTGGCTGGGT GTTTCTGAAA CTTTTTGATG CCACTGGAAT TCCTATTCCA 
GCAAAGACTT ACAAGCTCTT CTTGAACGGA GGCACCCCTT ATGAAAAAGG TGTTGAAGTG 
GACCCTTCAG TATCCAGAAG AGCACATGGT AGTGTTTTCC ATCAGTTGAT GACAATGAGG 
AGGCAGCCTC AACTTCTAGT CAAACTGAGA TCTTTGAACA GAAGATCCAG GAGCACACTA 
AGTCTACTAC CAGAAACATT AATCGGAAGC ATGTGCTGCG TTCATTTGCT GATATTCTAT 
CGCCAGATTC TTGGAGATGT TCTCCTGAAA GACAGGATGA GCCTGAACAG TGCAGACTTA 
ATTAGTAATC CAGTGCTGGC CACCTTCCCC AAGCTCTTGG AGGAGTCGGA CATAATGGAT 
GCACTCAGGA GTTCCTGGGC TGAGAAAGAG AGTACATTAA AAAGATCAGA GAAGGGAGAC 
AGGGAGCTCC TGAAGGCGGT GTTCCTGCTG GTTTACCACG ACTGCGCGCT CCCCCTGCTG 
CACTCTGCGC TCCTGCCCCC GTCTAGATGG GCAGAGGAGG AGACTGAGGC TGCCCGGTGG 
AAGGGCATCG CCGACTTCCT TAAGCAAAAC CAGGAAAATG AGGGCGCCCT CCAGGTTCTG 
CTGTCGCCAG AGGGGGTCCA CGAACCTTTC GACATTTCCG AGCAGACCTA TGACTTCTTG 
GGTGAAAGAA GAAAGAACGT AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212354.1
CDS	609..2615
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	XM_005212297.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 1 (NPHP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212297.4

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCCCTGAA ACTGGGGCAC TTGCTGAAAG AGAGGAAGAG 
GAGAACTCTG ATGAAGAAGA TGAGGAAGAA GACGACGACG AAGAAGAAAG TGAAGAAAGT 
GGGGGTGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAGGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTACGGCTCA GCAAGTGGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAACGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGGAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGATGG AGCCGAAGTC AAGCAAAGAA CTGATTCTCA CTGGAGTGCT 
GTCCGGAAGG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCCG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGTT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTTC TGAGCAACAT TCACACAGTC 
AGGGCCACGT GGCAACCTAA GAAGCCCAAA ACATGGACCT TTTCTCCCCA GGTTACTGGC 
ATCTTGCCCT GCCTGCTTGA TGGCGACTGT TTCATCAGAT CCAACTCTTC CTCTCCAGAT 
CTTGGAATAT TATTTGAACT TGGAATTTCT TATATTCGCA ATTCCACTGG TGAAAGAGGA 
GAGTTGAGCT GTGGCTGGGT GTTTCTGAAA CTTTTTGATG CCACTGGAAT TCCTATTCCA 
GCAAAGACTT ACAAGCTCTT CTTGAACGGA GGCACCCCTT ATGAAAAAGG TGTTGAAGTG 
GACCCTTCAG TATCCAGAAG AGCACATGGT AGTGTTTTCC ATCAGTTGAT GACAATGAGG 
AGGCAGCCTC AACTTCTAGT CAAACTGAGA TCTTTGAACA GAAGATCCAG GAGCACACTA 
AGTCTACTAC CAGAAACATT AATCGGAAGC ATGTGCTGCG TTCATTTGCT GATATTCTAT 
CGCCAGATTC TTGGAGATGT TCTCCTGAAA GACAGGATGA GCCTGAACAG TGCAGACTTA 
ATTAGTAATC CAGTGCTGGC CACCTTCCCC AAGCTCTTGG AGGAGTCGGA CATAATGGAT 
GCACTCAGGA GTTCCTGGGC TGAGAAAGAG AGTACATTAA AAAGATCAGA GAAGGGAGAC 
AGGGAGCTCC TGAAGGCGGT GTTCCTGCTG GTTTACCACG ACTGCGCGCT CCCCCTGCTG 
CACTCTGCGC TCCTGCCCCC GTCTAGATGG GCAGAGGAGG AGACTGAGGC TGCCCGGTGG 
AAGGGCATCG CCGACTTCCT TAAGCAAAAC CAGGAAAATG AGGGCGCCCT CCAGGTTCTG 
CTGTCGCCAG AGGGGGTCCA CGAACCTTTC GACATTTCCG AGCAGACCTA TGACTTCTTG 
GGTGAAAGAA GAAAGAACGT AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_005212298.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212355.1 Bos taurus nephrocystin 1 (NPHP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220184
Clone ID Related Accession (Same CDS sequence)	XM_005212298.4
Accession Version	XM_005212298.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212298.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAAA GATGTATCCA GTTAAAACAG GCAATAGATG AAAATAAAAA TGCTCTTCAA 
AAATTAAAAA AAGCTGATGA ACCTGCACCT GTGGGCAATT ATTATCAGAA GAAAGAAGAG 
GAGCACAATC TTTTGGATAA GCTTACCCAC CAACTGCAGG AGCTTGCTGT GTCCATAAGC 
AGAGAAAATG TCCCTGAAAC TGGGGCACTT GCTGAAAGAG AGGAAGAGGA GAACTCTGAT 
GAAGAAGATG AGGAAGAAGA CGACGACGAA GAAGAAAGTG AAGAAAGTGG GGGTGAGGAC 
GAAGAAACAG AAGAGGAAGA GGAAGAGAAA CAGGAAGGTG AATCTCAGCC AGAGGCCACG 
AGTGGAGAAT ACATTGCTCT TGGAGATTTT ACGGCTCAGC AAGTGGGAGA CCTGACATTT 
AAAAAAGGGG AAATTCTCTG TATAATCAAA AAAAACTCTG ATGGTTGGTG GATGGCTAAG 
AACGCCAAAG GAAACGAAGG CCTTATTCCT AAAACCTACT TGGAGCCCTA TAATAAAGAA 
GGAGGTCAAG AATCCAGTGA AGGAGGCAGT GAAGAAGATA CAGAGGTAGG GGATGGAACA 
GTGGATGGAG CCGAAGTCAA GCAAAGAACT GATTCTCACT GGAGTGCTGT CCGGAAGGCC 
ATCTCAGAGC AGATAAACAC CGTCGACGTG CTGACCGCGA TGGGAGCGAT TCCTGCCGGG 
TTCAGGCCTT CCACGCTCTT CCAGCTCCTG GAGGAAGGGA ATCAATTCCG AGCAAGTTAC 
TTCTTACAGC CAGAGCTCAC ACCTTCCCAA CTGGCCTTCA GAGATCTGAT GTGGGATGCA 
AAAACCGGCT CTATCAGGTC AAGACCGAGC CGTGTCTCCT TGATTCTGAC TCTCTGGAGC 
TGCAAGATGA TTCCTCTTCC AGGGGTGAGC ATCCAAGTTC TGAGCAGACA CGTGCGCCTC 
TGTCTGTTTG ACGGCAGTGA GGTTCTGAGC AACATTCACA CAGTCAGGGC CACGTGGCAA 
CCTAAGAAGC CCAAAACATG GACCTTTTCT CCCCAGGTTA CTGGCATCTT GCCCTGCCTG 
CTTGATGGCG ACTGTTTCAT CAGATCCAAC TCTTCCTCTC CAGATCTTGG AATATTATTT 
GAACTTGGAA TTTCTTATAT TCGCAATTCC ACTGGTGAAA GAGGAGAGTT GAGCTGTGGC 
TGGGTGTTTC TGAAACTTTT TGATGCCACT GGAATTCCTA TTCCAGCAAA GACTTACAAG 
CTCTTCTTGA ACGGAGGCAC CCCTTATGAA AAAGGTGTTG AAGTGGACCC TTCAGTATCC 
AGAAGAGCAC ATGGTAGTGT TTTCCATCAG TTGATGACAA TGAGGAGGCA GCCTCAACTT 
CTAGTCAAAC TGAGATCTTT GAACAGAAGA TCCAGGAGCA CACTAAGTCT ACTACCAGAA 
ACATTAATCG GAAGCATGTG CTGCGTTCAT TTGCTGATAT TCTATCGCCA GATTCTTGGA 
GATGTTCTCC TGAAAGACAG GATGAGCCTG AACAGTGCAG ACTTAATTAG TAATCCAGTG 
CTGGCCACCT TCCCCAAGCT CTTGGAGGAG TCGGACATAA TGGATGCACT CAGGAGTTCC 
TGGGCTGAGA AAGAGAGTAC ATTAAAAAGA TCAGAGAAGG GAGACAGGGA GCTCCTGAAG 
GCGGTGTTCC TGCTGGTTTA CCACGACTGC GCGCTCCCCC TGCTGCACTC TGCGCTCCTG 
CCCCCGTCTA GATGGGCAGA GGAGGAGACT GAGGCTGCCC GGTGGAAGGG CATCGCCGAC 
TTCCTTAAGC AAAACCAGGA AAATGAGGGC GCCCTCCAGG TTCTGCTGTC GCCAGAGGGG 
GTCCACGAAC CTTTCGACAT TTCCGAGCAG ACCTATGACT TCTTGGGTGA AAGAAGAAAG 
AACGTAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212355.1
CDS	212..2143
Translation	MYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVPETGALAEREEEENSDEEDEEEDDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFTAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEGGSEEDTEVGDGTVDGAEVKQRTDSHWSAVRKAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDATGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSRRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVT

Target ORF information:

RefSeq Version	XM_005212298.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 1 (NPHP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212298.4

ORF Insert Sequence:

ATGTACCAAA GATGTATCCA GTTAAAACAG GCAATAGATG AAAATAAAAA TGCTCTTCAA 
AAATTAAAAA AAGCTGATGA ACCTGCACCT GTGGGCAATT ATTATCAGAA GAAAGAAGAG 
GAGCACAATC TTTTGGATAA GCTTACCCAC CAACTGCAGG AGCTTGCTGT GTCCATAAGC 
AGAGAAAATG TCCCTGAAAC TGGGGCACTT GCTGAAAGAG AGGAAGAGGA GAACTCTGAT 
GAAGAAGATG AGGAAGAAGA CGACGACGAA GAAGAAAGTG AAGAAAGTGG GGGTGAGGAC 
GAAGAAACAG AAGAGGAAGA GGAAGAGAAA CAGGAAGGTG AATCTCAGCC AGAGGCCACG 
AGTGGAGAAT ACATTGCTCT TGGAGATTTT ACGGCTCAGC AAGTGGGAGA CCTGACATTT 
AAAAAAGGGG AAATTCTCTG TATAATCAAA AAAAACTCTG ATGGTTGGTG GATGGCTAAG 
AACGCCAAAG GAAACGAAGG CCTTATTCCT AAAACCTACT TGGAGCCCTA TAATAAAGAA 
GGAGGTCAAG AATCCAGTGA AGGAGGCAGT GAAGAAGATA CAGAGGTAGG GGATGGAACA 
GTGGATGGAG CCGAAGTCAA GCAAAGAACT GATTCTCACT GGAGTGCTGT CCGGAAGGCC 
ATCTCAGAGC AGATAAACAC CGTCGACGTG CTGACCGCGA TGGGAGCGAT TCCTGCCGGG 
TTCAGGCCTT CCACGCTCTT CCAGCTCCTG GAGGAAGGGA ATCAATTCCG AGCAAGTTAC 
TTCTTACAGC CAGAGCTCAC ACCTTCCCAA CTGGCCTTCA GAGATCTGAT GTGGGATGCA 
AAAACCGGCT CTATCAGGTC AAGACCGAGC CGTGTCTCCT TGATTCTGAC TCTCTGGAGC 
TGCAAGATGA TTCCTCTTCC AGGGGTGAGC ATCCAAGTTC TGAGCAGACA CGTGCGCCTC 
TGTCTGTTTG ACGGCAGTGA GGTTCTGAGC AACATTCACA CAGTCAGGGC CACGTGGCAA 
CCTAAGAAGC CCAAAACATG GACCTTTTCT CCCCAGGTTA CTGGCATCTT GCCCTGCCTG 
CTTGATGGCG ACTGTTTCAT CAGATCCAAC TCTTCCTCTC CAGATCTTGG AATATTATTT 
GAACTTGGAA TTTCTTATAT TCGCAATTCC ACTGGTGAAA GAGGAGAGTT GAGCTGTGGC 
TGGGTGTTTC TGAAACTTTT TGATGCCACT GGAATTCCTA TTCCAGCAAA GACTTACAAG 
CTCTTCTTGA ACGGAGGCAC CCCTTATGAA AAAGGTGTTG AAGTGGACCC TTCAGTATCC 
AGAAGAGCAC ATGGTAGTGT TTTCCATCAG TTGATGACAA TGAGGAGGCA GCCTCAACTT 
CTAGTCAAAC TGAGATCTTT GAACAGAAGA TCCAGGAGCA CACTAAGTCT ACTACCAGAA 
ACATTAATCG GAAGCATGTG CTGCGTTCAT TTGCTGATAT TCTATCGCCA GATTCTTGGA 
GATGTTCTCC TGAAAGACAG GATGAGCCTG AACAGTGCAG ACTTAATTAG TAATCCAGTG 
CTGGCCACCT TCCCCAAGCT CTTGGAGGAG TCGGACATAA TGGATGCACT CAGGAGTTCC 
TGGGCTGAGA AAGAGAGTAC ATTAAAAAGA TCAGAGAAGG GAGACAGGGA GCTCCTGAAG 
GCGGTGTTCC TGCTGGTTTA CCACGACTGC GCGCTCCCCC TGCTGCACTC TGCGCTCCTG 
CCCCCGTCTA GATGGGCAGA GGAGGAGACT GAGGCTGCCC GGTGGAAGGG CATCGCCGAC 
TTCCTTAAGC AAAACCAGGA AAATGAGGGC GCCCTCCAGG TTCTGCTGTC GCCAGAGGGG 
GTCCACGAAC CTTTCGACAT TTCCGAGCAG ACCTATGACT TCTTGGGTGA AAGAAGAAAG 
AACGTAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NPHP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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