gRNAs
The following NPHP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OCe00282 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001195841.1 | |
| Accession Version | NM_001195841.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-12-14 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nephrocystin-1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAEX03010899.1. On Sep 30, 2010 this sequence version replaced XM_532954.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001182770.1 |
| CDS | 1..2085 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001195841.1 |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe18573 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630063.2 , XM_005630063.3 | |
| Accession Version | XM_005630063.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-16 | |
| Organism | Canis lupus familiaris(Dog) | |
| Product | nephrocystin-1 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630120.1 |
| CDS | 58..2139 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630063.2 |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe18573 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005630063.2 , XM_005630063.3 | |
| Accession Version | XM_005630063.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nephrocystin-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005630120.1 |
| CDS | 256..2337 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005630063.3 |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OCe294143 | |
| Clone ID Related Accession (Same CDS sequence) | XM_022404318.1 | |
| Accession Version | XM_022404318.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-04 | |
| Organism | Canis lupus familiaris(dog) | |
| Product | nephrocystin-1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_022260026.1 |
| CDS | 257..2239 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_022404318.1 |
1 | ATGCTGGCTA GGCGGCAGAG CAATCGTCTG CAGGCCCTGC AGCGCCGCAG TCAGGAATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Crk-associated substrate p130(Cas) interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells. |
