AHCTF1 cDNA ORF clone, Bos taurus(cattle)

The following AHCTF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AHCTF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223471 NM_001192036.2
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223470 XM_024976087.1
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223470 XM_024976091.1
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223470 XM_024976088.1
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223471 XM_005216798.4
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223472 XM_024976089.1
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223473 XM_024976090.1
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa27067 NM_001192036.1
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27067 XM_005216798.3
Latest version!
Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223471
    Clone ID Related Accession (Same CDS sequence) NM_001192036.2 , XM_005216798.4
    Accession Version NM_001192036.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product protein ELYS
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On Dec 20, 2018 this sequence version replaced NM_001192036.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GGVB01105535.1, SRR6764281.20600.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CAGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC AGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGCAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178965.2
    CDS1..6831
    Translation

    Target ORF information:

    RefSeq Version NM_001192036.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192036.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CAGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC AGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGCAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223470
    Clone ID Related Accession (Same CDS sequence) XM_024976091.1 , XM_024976087.1 , XM_024976088.1
    Accession Version XM_024976091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGGTAACAAT
    AAATCCACGC CCGAAGTCCC TGGTGATAGT GGAAGATCTG GGCCTGTTAC CTCTGAAAGC
    CCTGTAGTCT CTGAACATAG GCTTGACCAG GAAGTAGAGT TGAACTTAAA AGAAGATCAT
    GAAATAGAAG TTGATGTACT AAAAGCAAGT GTTGACTTAC CAGAAGAAAA GACTCCAATT
    TCTGATGATC CTCCTGATAC TCAAGAAATT CATGTAATTG AACATGAAAA GCTTGAAGGT
    CAAGATTCAG GACAAGAGGC TAGGAATCTT TCATTTAATG AGTTGTATCC CTCAGGAACT
    CTTAAGCTTC AATACAATTT TGATACCATT GAGCAACAGT TTTGTGACTT ACCTGATAAT
    AAAGACTCTG CTGAGTGTGA CATTGCTGAG GTGGATGGAG AACATTTTGT GGCACAGAGC
    AACTTTACCT TGATTTTGGA AGGTGAAGAA GGAGAAGTAG AGACAGATGA TTCTGCAGCA
    TCTGATGTGT TAGCTAAAGC AGCTAATGCA GCCACTGAGG AAAAACATGT ATGCAGTGAG
    GAAAGTGAGA ATCACAGGCA TGTTGCAGAT TTGCCATCTG TCATAATGAG TGATCAGAAG
    TCCCAGAGGG TAGAGACATT ACCATATGTG CCTGAACCAA TTAAAGTAGC AATTGCAGAA
    AATTTATTAG ATGTAATTAA AGACACAAGA AGTAAAGAAA TTACTTCAGA TACAATGGAA
    CAGTCCATTC AAGAAAACAT ACCTTTAATA AGCCAAAAGG TAAAGTGTTC CACCAAGTTA
    GCCAACTTTA CAACTAAGAC TGTTCAGGAA ACAAGTACAG AGACTATAAA TGTCAGCGAG
    GTTAATGACA TGATTTCCTC CAGAACTCGC AAGAGAGGAC GTGTCCAAAA CCTGAATGTC
    AAATCAGCAC AACAGGAAGA GCCAGCCGCT GTTGCTACTC CTGAGATGCT GGGGCTTTCA
    GTTACAAAGA AAACTAGGAA AACAAAAGAA ATTGCAGGGG CTCTGGAAAG TGCCAGTTCT
    GATGACAAGG GAGTATCTCA TAACCAGCAA ATACCTCAAA ATGCTATTAC TCCTAGGAGA
    GGAAGGAGAA AAAAAGATGT TAATCAAGAC ACATTAGAAA ATGCTAATTC TGTGGAACAA
    GAATTACAAG TTACTCCAGG TAGGGAATTA AGAAGGTTAA AATCATCTCA GCTGTTGGAA
    GCAGCAACTG AAGAAAGTTC TTTTAGAAAA GAAGTTAAGC TTTCATCTGC TACAAAAAGG
    ACTCCTAGAA GAACTAAATC TGTAGAAAAT CGGGAAAGTG TTGAAGTTAT AAATGATCTG
    AAGGTCAGCA AAGTAGCAGG TCCTAGCAGA AGTACCAGAA AATTGAGAAG TACTCATTTG
    GAAGCTTCTG AAAGTGTAGG ATATAAACAA GAGGGGGAGT CCGCTGAACA ACAGCTGCCT
    ATTCAACGTG GTAAAAGGGT TAAAAAGAAA GAAGTTAGTG CGTTGGATGT TACAGAAGAT
    TCCAAACTTG ATTCCTCCCA GTTGACTATT CAAGCAGATT TTGGTACACC TGCTACACCT
    AGGAAACGTG GTAGACCAAG AAAAATCAAT CCATCTGAAG ATGTAGAATC TAAGGCTGTT
    ACAGAAGCAG GAAGTCTCAA GAAGAGAGAA GGTCTCAGCA TCCAAAGGAG ATCCACAAGA
    AACACCCCAG CTAAAAGTGA AAATACTGAT GTTGGGAAAC CAACTTTAGA AGAGTCAGTT
    TTCATGCCAA ATGAGGAAGT TGCTGCAGTG ATGAGCTCTA AGAAGCAGCT TACAAAAAGG
    ACTGAAAACC AAAGCCAGAA ACGTTCATTA CGTTCAATAC CAGAAAAGTG CATAGATGAA
    GAAACGGCAC ACACAGAGCC AGATGCCCAA GAAGAAAGAT TGCTTGCTAC AGTTGCTTTG
    ACTAAATCTT CCCGTAGCAC ACGGACTAGA TCTACCAAGG CCATCTTGTT GCCGGACCTT
    TCTGAACCAA AAAATGAGTC TCTTTTATTT TCTCCTCCTG TGTCAAAGAT TCCAAGGAAA
    GAGAAAGCTA AAAAAATAGA GGCTCCTGCA CAGCTGCAAG AATTAGTTTC AGACTTACCT
    TCTCAATTTG TCTCCTCACC TCCTGCTTTA AGGACCAGGC GGAAAAACCC ATTGAATACA
    TCCAAACATA CAGATGGACT GGAAGATGAT GGTCAAACAA CTGAAACTGT GGGAAAGCAA
    CAAGTGAAAA GAATCAGGAC TGCAAAAACA AAACAAGCAA GCAAAAACAC AGAAAAAGAA
    AGTTGGTCAC CTCCCCCGGT AGAAATTAAG CTGATTTCTC CCTTGGCAAG CCCAGTTGAT
    GGAGTCAAGA GCAAACCAAG AAAGACTGCA GAAGTAACGG GAAAAACTGT TGGAAGGAGC
    AGGAAGAAGT TGTCTTCTTT TCCAAAGCAA GTTCTACGCA GAAAAATGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831859.1
    CDS396..7229
    Translation

    Target ORF information:

    RefSeq Version XM_024976091.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGGTAACAAT
    AAATCCACGC CCGAAGTCCC TGGTGATAGT GGAAGATCTG GGCCTGTTAC CTCTGAAAGC
    CCTGTAGTCT CTGAACATAG GCTTGACCAG GAAGTAGAGT TGAACTTAAA AGAAGATCAT
    GAAATAGAAG TTGATGTACT AAAAGCAAGT GTTGACTTAC CAGAAGAAAA GACTCCAATT
    TCTGATGATC CTCCTGATAC TCAAGAAATT CATGTAATTG AACATGAAAA GCTTGAAGGT
    CAAGATTCAG GACAAGAGGC TAGGAATCTT TCATTTAATG AGTTGTATCC CTCAGGAACT
    CTTAAGCTTC AATACAATTT TGATACCATT GAGCAACAGT TTTGTGACTT ACCTGATAAT
    AAAGACTCTG CTGAGTGTGA CATTGCTGAG GTGGATGGAG AACATTTTGT GGCACAGAGC
    AACTTTACCT TGATTTTGGA AGGTGAAGAA GGAGAAGTAG AGACAGATGA TTCTGCAGCA
    TCTGATGTGT TAGCTAAAGC AGCTAATGCA GCCACTGAGG AAAAACATGT ATGCAGTGAG
    GAAAGTGAGA ATCACAGGCA TGTTGCAGAT TTGCCATCTG TCATAATGAG TGATCAGAAG
    TCCCAGAGGG TAGAGACATT ACCATATGTG CCTGAACCAA TTAAAGTAGC AATTGCAGAA
    AATTTATTAG ATGTAATTAA AGACACAAGA AGTAAAGAAA TTACTTCAGA TACAATGGAA
    CAGTCCATTC AAGAAAACAT ACCTTTAATA AGCCAAAAGG TAAAGTGTTC CACCAAGTTA
    GCCAACTTTA CAACTAAGAC TGTTCAGGAA ACAAGTACAG AGACTATAAA TGTCAGCGAG
    GTTAATGACA TGATTTCCTC CAGAACTCGC AAGAGAGGAC GTGTCCAAAA CCTGAATGTC
    AAATCAGCAC AACAGGAAGA GCCAGCCGCT GTTGCTACTC CTGAGATGCT GGGGCTTTCA
    GTTACAAAGA AAACTAGGAA AACAAAAGAA ATTGCAGGGG CTCTGGAAAG TGCCAGTTCT
    GATGACAAGG GAGTATCTCA TAACCAGCAA ATACCTCAAA ATGCTATTAC TCCTAGGAGA
    GGAAGGAGAA AAAAAGATGT TAATCAAGAC ACATTAGAAA ATGCTAATTC TGTGGAACAA
    GAATTACAAG TTACTCCAGG TAGGGAATTA AGAAGGTTAA AATCATCTCA GCTGTTGGAA
    GCAGCAACTG AAGAAAGTTC TTTTAGAAAA GAAGTTAAGC TTTCATCTGC TACAAAAAGG
    ACTCCTAGAA GAACTAAATC TGTAGAAAAT CGGGAAAGTG TTGAAGTTAT AAATGATCTG
    AAGGTCAGCA AAGTAGCAGG TCCTAGCAGA AGTACCAGAA AATTGAGAAG TACTCATTTG
    GAAGCTTCTG AAAGTGTAGG ATATAAACAA GAGGGGGAGT CCGCTGAACA ACAGCTGCCT
    ATTCAACGTG GTAAAAGGGT TAAAAAGAAA GAAGTTAGTG CGTTGGATGT TACAGAAGAT
    TCCAAACTTG ATTCCTCCCA GTTGACTATT CAAGCAGATT TTGGTACACC TGCTACACCT
    AGGAAACGTG GTAGACCAAG AAAAATCAAT CCATCTGAAG ATGTAGAATC TAAGGCTGTT
    ACAGAAGCAG GAAGTCTCAA GAAGAGAGAA GGTCTCAGCA TCCAAAGGAG ATCCACAAGA
    AACACCCCAG CTAAAAGTGA AAATACTGAT GTTGGGAAAC CAACTTTAGA AGAGTCAGTT
    TTCATGCCAA ATGAGGAAGT TGCTGCAGTG ATGAGCTCTA AGAAGCAGCT TACAAAAAGG
    ACTGAAAACC AAAGCCAGAA ACGTTCATTA CGTTCAATAC CAGAAAAGTG CATAGATGAA
    GAAACGGCAC ACACAGAGCC AGATGCCCAA GAAGAAAGAT TGCTTGCTAC AGTTGCTTTG
    ACTAAATCTT CCCGTAGCAC ACGGACTAGA TCTACCAAGG CCATCTTGTT GCCGGACCTT
    TCTGAACCAA AAAATGAGTC TCTTTTATTT TCTCCTCCTG TGTCAAAGAT TCCAAGGAAA
    GAGAAAGCTA AAAAAATAGA GGCTCCTGCA CAGCTGCAAG AATTAGTTTC AGACTTACCT
    TCTCAATTTG TCTCCTCACC TCCTGCTTTA AGGACCAGGC GGAAAAACCC ATTGAATACA
    TCCAAACATA CAGATGGACT GGAAGATGAT GGTCAAACAA CTGAAACTGT GGGAAAGCAA
    CAAGTGAAAA GAATCAGGAC TGCAAAAACA AAACAAGCAA GCAAAAACAC AGAAAAAGAA
    AGTTGGTCAC CTCCCCCGGT AGAAATTAAG CTGATTTCTC CCTTGGCAAG CCCAGTTGAT
    GGAGTCAAGA GCAAACCAAG AAAGACTGCA GAAGTAACGG GAAAAACTGT TGGAAGGAGC
    AGGAAGAAGT TGTCTTCTTT TCCAAAGCAA GTTCTACGCA GAAAAATGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223470
    Clone ID Related Accession (Same CDS sequence) XM_024976091.1 , XM_024976087.1 , XM_024976088.1
    Accession Version XM_024976087.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGGTAACAAT
    AAATCCACGC CCGAAGTCCC TGGTGATAGT GGAAGATCTG GGCCTGTTAC CTCTGAAAGC
    CCTGTAGTCT CTGAACATAG GCTTGACCAG GAAGTAGAGT TGAACTTAAA AGAAGATCAT
    GAAATAGAAG TTGATGTACT AAAAGCAAGT GTTGACTTAC CAGAAGAAAA GACTCCAATT
    TCTGATGATC CTCCTGATAC TCAAGAAATT CATGTAATTG AACATGAAAA GCTTGAAGGT
    CAAGATTCAG GACAAGAGGC TAGGAATCTT TCATTTAATG AGTTGTATCC CTCAGGAACT
    CTTAAGCTTC AATACAATTT TGATACCATT GAGCAACAGT TTTGTGACTT ACCTGATAAT
    AAAGACTCTG CTGAGTGTGA CATTGCTGAG GTGGATGGAG AACATTTTGT GGCACAGAGC
    AACTTTACCT TGATTTTGGA AGGTGAAGAA GGAGAAGTAG AGACAGATGA TTCTGCAGCA
    TCTGATGTGT TAGCTAAAGC AGCTAATGCA GCCACTGAGG AAAAACATGT ATGCAGTGAG
    GAAAGTGAGA ATCACAGGCA TGTTGCAGAT TTGCCATCTG TCATAATGAG TGATCAGAAG
    TCCCAGAGGG TAGAGACATT ACCATATGTG CCTGAACCAA TTAAAGTAGC AATTGCAGAA
    AATTTATTAG ATGTAATTAA AGACACAAGA AGTAAAGAAA TTACTTCAGA TACAATGGAA
    CAGTCCATTC AAGAAAACAT ACCTTTAATA AGCCAAAAGG TAAAGTGTTC CACCAAGTTA
    GCCAACTTTA CAACTAAGAC TGTTCAGGAA ACAAGTACAG AGACTATAAA TGTCAGCGAG
    GTTAATGACA TGATTTCCTC CAGAACTCGC AAGAGAGGAC GTGTCCAAAA CCTGAATGTC
    AAATCAGCAC AACAGGAAGA GCCAGCCGCT GTTGCTACTC CTGAGATGCT GGGGCTTTCA
    GTTACAAAGA AAACTAGGAA AACAAAAGAA ATTGCAGGGG CTCTGGAAAG TGCCAGTTCT
    GATGACAAGG GAGTATCTCA TAACCAGCAA ATACCTCAAA ATGCTATTAC TCCTAGGAGA
    GGAAGGAGAA AAAAAGATGT TAATCAAGAC ACATTAGAAA ATGCTAATTC TGTGGAACAA
    GAATTACAAG TTACTCCAGG TAGGGAATTA AGAAGGTTAA AATCATCTCA GCTGTTGGAA
    GCAGCAACTG AAGAAAGTTC TTTTAGAAAA GAAGTTAAGC TTTCATCTGC TACAAAAAGG
    ACTCCTAGAA GAACTAAATC TGTAGAAAAT CGGGAAAGTG TTGAAGTTAT AAATGATCTG
    AAGGTCAGCA AAGTAGCAGG TCCTAGCAGA AGTACCAGAA AATTGAGAAG TACTCATTTG
    GAAGCTTCTG AAAGTGTAGG ATATAAACAA GAGGGGGAGT CCGCTGAACA ACAGCTGCCT
    ATTCAACGTG GTAAAAGGGT TAAAAAGAAA GAAGTTAGTG CGTTGGATGT TACAGAAGAT
    TCCAAACTTG ATTCCTCCCA GTTGACTATT CAAGCAGATT TTGGTACACC TGCTACACCT
    AGGAAACGTG GTAGACCAAG AAAAATCAAT CCATCTGAAG ATGTAGAATC TAAGGCTGTT
    ACAGAAGCAG GAAGTCTCAA GAAGAGAGAA GGTCTCAGCA TCCAAAGGAG ATCCACAAGA
    AACACCCCAG CTAAAAGTGA AAATACTGAT GTTGGGAAAC CAACTTTAGA AGAGTCAGTT
    TTCATGCCAA ATGAGGAAGT TGCTGCAGTG ATGAGCTCTA AGAAGCAGCT TACAAAAAGG
    ACTGAAAACC AAAGCCAGAA ACGTTCATTA CGTTCAATAC CAGAAAAGTG CATAGATGAA
    GAAACGGCAC ACACAGAGCC AGATGCCCAA GAAGAAAGAT TGCTTGCTAC AGTTGCTTTG
    ACTAAATCTT CCCGTAGCAC ACGGACTAGA TCTACCAAGG CCATCTTGTT GCCGGACCTT
    TCTGAACCAA AAAATGAGTC TCTTTTATTT TCTCCTCCTG TGTCAAAGAT TCCAAGGAAA
    GAGAAAGCTA AAAAAATAGA GGCTCCTGCA CAGCTGCAAG AATTAGTTTC AGACTTACCT
    TCTCAATTTG TCTCCTCACC TCCTGCTTTA AGGACCAGGC GGAAAAACCC ATTGAATACA
    TCCAAACATA CAGATGGACT GGAAGATGAT GGTCAAACAA CTGAAACTGT GGGAAAGCAA
    CAAGTGAAAA GAATCAGGAC TGCAAAAACA AAACAAGCAA GCAAAAACAC AGAAAAAGAA
    AGTTGGTCAC CTCCCCCGGT AGAAATTAAG CTGATTTCTC CCTTGGCAAG CCCAGTTGAT
    GGAGTCAAGA GCAAACCAAG AAAGACTGCA GAAGTAACGG GAAAAACTGT TGGAAGGAGC
    AGGAAGAAGT TGTCTTCTTT TCCAAAGCAA GTTCTACGCA GAAAAATGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831855.1
    CDS28..6861
    Translation

    Target ORF information:

    RefSeq Version XM_024976087.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976087.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGGTAACAAT
    AAATCCACGC CCGAAGTCCC TGGTGATAGT GGAAGATCTG GGCCTGTTAC CTCTGAAAGC
    CCTGTAGTCT CTGAACATAG GCTTGACCAG GAAGTAGAGT TGAACTTAAA AGAAGATCAT
    GAAATAGAAG TTGATGTACT AAAAGCAAGT GTTGACTTAC CAGAAGAAAA GACTCCAATT
    TCTGATGATC CTCCTGATAC TCAAGAAATT CATGTAATTG AACATGAAAA GCTTGAAGGT
    CAAGATTCAG GACAAGAGGC TAGGAATCTT TCATTTAATG AGTTGTATCC CTCAGGAACT
    CTTAAGCTTC AATACAATTT TGATACCATT GAGCAACAGT TTTGTGACTT ACCTGATAAT
    AAAGACTCTG CTGAGTGTGA CATTGCTGAG GTGGATGGAG AACATTTTGT GGCACAGAGC
    AACTTTACCT TGATTTTGGA AGGTGAAGAA GGAGAAGTAG AGACAGATGA TTCTGCAGCA
    TCTGATGTGT TAGCTAAAGC AGCTAATGCA GCCACTGAGG AAAAACATGT ATGCAGTGAG
    GAAAGTGAGA ATCACAGGCA TGTTGCAGAT TTGCCATCTG TCATAATGAG TGATCAGAAG
    TCCCAGAGGG TAGAGACATT ACCATATGTG CCTGAACCAA TTAAAGTAGC AATTGCAGAA
    AATTTATTAG ATGTAATTAA AGACACAAGA AGTAAAGAAA TTACTTCAGA TACAATGGAA
    CAGTCCATTC AAGAAAACAT ACCTTTAATA AGCCAAAAGG TAAAGTGTTC CACCAAGTTA
    GCCAACTTTA CAACTAAGAC TGTTCAGGAA ACAAGTACAG AGACTATAAA TGTCAGCGAG
    GTTAATGACA TGATTTCCTC CAGAACTCGC AAGAGAGGAC GTGTCCAAAA CCTGAATGTC
    AAATCAGCAC AACAGGAAGA GCCAGCCGCT GTTGCTACTC CTGAGATGCT GGGGCTTTCA
    GTTACAAAGA AAACTAGGAA AACAAAAGAA ATTGCAGGGG CTCTGGAAAG TGCCAGTTCT
    GATGACAAGG GAGTATCTCA TAACCAGCAA ATACCTCAAA ATGCTATTAC TCCTAGGAGA
    GGAAGGAGAA AAAAAGATGT TAATCAAGAC ACATTAGAAA ATGCTAATTC TGTGGAACAA
    GAATTACAAG TTACTCCAGG TAGGGAATTA AGAAGGTTAA AATCATCTCA GCTGTTGGAA
    GCAGCAACTG AAGAAAGTTC TTTTAGAAAA GAAGTTAAGC TTTCATCTGC TACAAAAAGG
    ACTCCTAGAA GAACTAAATC TGTAGAAAAT CGGGAAAGTG TTGAAGTTAT AAATGATCTG
    AAGGTCAGCA AAGTAGCAGG TCCTAGCAGA AGTACCAGAA AATTGAGAAG TACTCATTTG
    GAAGCTTCTG AAAGTGTAGG ATATAAACAA GAGGGGGAGT CCGCTGAACA ACAGCTGCCT
    ATTCAACGTG GTAAAAGGGT TAAAAAGAAA GAAGTTAGTG CGTTGGATGT TACAGAAGAT
    TCCAAACTTG ATTCCTCCCA GTTGACTATT CAAGCAGATT TTGGTACACC TGCTACACCT
    AGGAAACGTG GTAGACCAAG AAAAATCAAT CCATCTGAAG ATGTAGAATC TAAGGCTGTT
    ACAGAAGCAG GAAGTCTCAA GAAGAGAGAA GGTCTCAGCA TCCAAAGGAG ATCCACAAGA
    AACACCCCAG CTAAAAGTGA AAATACTGAT GTTGGGAAAC CAACTTTAGA AGAGTCAGTT
    TTCATGCCAA ATGAGGAAGT TGCTGCAGTG ATGAGCTCTA AGAAGCAGCT TACAAAAAGG
    ACTGAAAACC AAAGCCAGAA ACGTTCATTA CGTTCAATAC CAGAAAAGTG CATAGATGAA
    GAAACGGCAC ACACAGAGCC AGATGCCCAA GAAGAAAGAT TGCTTGCTAC AGTTGCTTTG
    ACTAAATCTT CCCGTAGCAC ACGGACTAGA TCTACCAAGG CCATCTTGTT GCCGGACCTT
    TCTGAACCAA AAAATGAGTC TCTTTTATTT TCTCCTCCTG TGTCAAAGAT TCCAAGGAAA
    GAGAAAGCTA AAAAAATAGA GGCTCCTGCA CAGCTGCAAG AATTAGTTTC AGACTTACCT
    TCTCAATTTG TCTCCTCACC TCCTGCTTTA AGGACCAGGC GGAAAAACCC ATTGAATACA
    TCCAAACATA CAGATGGACT GGAAGATGAT GGTCAAACAA CTGAAACTGT GGGAAAGCAA
    CAAGTGAAAA GAATCAGGAC TGCAAAAACA AAACAAGCAA GCAAAAACAC AGAAAAAGAA
    AGTTGGTCAC CTCCCCCGGT AGAAATTAAG CTGATTTCTC CCTTGGCAAG CCCAGTTGAT
    GGAGTCAAGA GCAAACCAAG AAAGACTGCA GAAGTAACGG GAAAAACTGT TGGAAGGAGC
    AGGAAGAAGT TGTCTTCTTT TCCAAAGCAA GTTCTACGCA GAAAAATGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223470
    Clone ID Related Accession (Same CDS sequence) XM_024976091.1 , XM_024976087.1 , XM_024976088.1
    Accession Version XM_024976088.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGGTAACAAT
    AAATCCACGC CCGAAGTCCC TGGTGATAGT GGAAGATCTG GGCCTGTTAC CTCTGAAAGC
    CCTGTAGTCT CTGAACATAG GCTTGACCAG GAAGTAGAGT TGAACTTAAA AGAAGATCAT
    GAAATAGAAG TTGATGTACT AAAAGCAAGT GTTGACTTAC CAGAAGAAAA GACTCCAATT
    TCTGATGATC CTCCTGATAC TCAAGAAATT CATGTAATTG AACATGAAAA GCTTGAAGGT
    CAAGATTCAG GACAAGAGGC TAGGAATCTT TCATTTAATG AGTTGTATCC CTCAGGAACT
    CTTAAGCTTC AATACAATTT TGATACCATT GAGCAACAGT TTTGTGACTT ACCTGATAAT
    AAAGACTCTG CTGAGTGTGA CATTGCTGAG GTGGATGGAG AACATTTTGT GGCACAGAGC
    AACTTTACCT TGATTTTGGA AGGTGAAGAA GGAGAAGTAG AGACAGATGA TTCTGCAGCA
    TCTGATGTGT TAGCTAAAGC AGCTAATGCA GCCACTGAGG AAAAACATGT ATGCAGTGAG
    GAAAGTGAGA ATCACAGGCA TGTTGCAGAT TTGCCATCTG TCATAATGAG TGATCAGAAG
    TCCCAGAGGG TAGAGACATT ACCATATGTG CCTGAACCAA TTAAAGTAGC AATTGCAGAA
    AATTTATTAG ATGTAATTAA AGACACAAGA AGTAAAGAAA TTACTTCAGA TACAATGGAA
    CAGTCCATTC AAGAAAACAT ACCTTTAATA AGCCAAAAGG TAAAGTGTTC CACCAAGTTA
    GCCAACTTTA CAACTAAGAC TGTTCAGGAA ACAAGTACAG AGACTATAAA TGTCAGCGAG
    GTTAATGACA TGATTTCCTC CAGAACTCGC AAGAGAGGAC GTGTCCAAAA CCTGAATGTC
    AAATCAGCAC AACAGGAAGA GCCAGCCGCT GTTGCTACTC CTGAGATGCT GGGGCTTTCA
    GTTACAAAGA AAACTAGGAA AACAAAAGAA ATTGCAGGGG CTCTGGAAAG TGCCAGTTCT
    GATGACAAGG GAGTATCTCA TAACCAGCAA ATACCTCAAA ATGCTATTAC TCCTAGGAGA
    GGAAGGAGAA AAAAAGATGT TAATCAAGAC ACATTAGAAA ATGCTAATTC TGTGGAACAA
    GAATTACAAG TTACTCCAGG TAGGGAATTA AGAAGGTTAA AATCATCTCA GCTGTTGGAA
    GCAGCAACTG AAGAAAGTTC TTTTAGAAAA GAAGTTAAGC TTTCATCTGC TACAAAAAGG
    ACTCCTAGAA GAACTAAATC TGTAGAAAAT CGGGAAAGTG TTGAAGTTAT AAATGATCTG
    AAGGTCAGCA AAGTAGCAGG TCCTAGCAGA AGTACCAGAA AATTGAGAAG TACTCATTTG
    GAAGCTTCTG AAAGTGTAGG ATATAAACAA GAGGGGGAGT CCGCTGAACA ACAGCTGCCT
    ATTCAACGTG GTAAAAGGGT TAAAAAGAAA GAAGTTAGTG CGTTGGATGT TACAGAAGAT
    TCCAAACTTG ATTCCTCCCA GTTGACTATT CAAGCAGATT TTGGTACACC TGCTACACCT
    AGGAAACGTG GTAGACCAAG AAAAATCAAT CCATCTGAAG ATGTAGAATC TAAGGCTGTT
    ACAGAAGCAG GAAGTCTCAA GAAGAGAGAA GGTCTCAGCA TCCAAAGGAG ATCCACAAGA
    AACACCCCAG CTAAAAGTGA AAATACTGAT GTTGGGAAAC CAACTTTAGA AGAGTCAGTT
    TTCATGCCAA ATGAGGAAGT TGCTGCAGTG ATGAGCTCTA AGAAGCAGCT TACAAAAAGG
    ACTGAAAACC AAAGCCAGAA ACGTTCATTA CGTTCAATAC CAGAAAAGTG CATAGATGAA
    GAAACGGCAC ACACAGAGCC AGATGCCCAA GAAGAAAGAT TGCTTGCTAC AGTTGCTTTG
    ACTAAATCTT CCCGTAGCAC ACGGACTAGA TCTACCAAGG CCATCTTGTT GCCGGACCTT
    TCTGAACCAA AAAATGAGTC TCTTTTATTT TCTCCTCCTG TGTCAAAGAT TCCAAGGAAA
    GAGAAAGCTA AAAAAATAGA GGCTCCTGCA CAGCTGCAAG AATTAGTTTC AGACTTACCT
    TCTCAATTTG TCTCCTCACC TCCTGCTTTA AGGACCAGGC GGAAAAACCC ATTGAATACA
    TCCAAACATA CAGATGGACT GGAAGATGAT GGTCAAACAA CTGAAACTGT GGGAAAGCAA
    CAAGTGAAAA GAATCAGGAC TGCAAAAACA AAACAAGCAA GCAAAAACAC AGAAAAAGAA
    AGTTGGTCAC CTCCCCCGGT AGAAATTAAG CTGATTTCTC CCTTGGCAAG CCCAGTTGAT
    GGAGTCAAGA GCAAACCAAG AAAGACTGCA GAAGTAACGG GAAAAACTGT TGGAAGGAGC
    AGGAAGAAGT TGTCTTCTTT TCCAAAGCAA GTTCTACGCA GAAAAATGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831856.1
    CDS140..6973
    Translation

    Target ORF information:

    RefSeq Version XM_024976088.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976088.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGGTAACAAT
    AAATCCACGC CCGAAGTCCC TGGTGATAGT GGAAGATCTG GGCCTGTTAC CTCTGAAAGC
    CCTGTAGTCT CTGAACATAG GCTTGACCAG GAAGTAGAGT TGAACTTAAA AGAAGATCAT
    GAAATAGAAG TTGATGTACT AAAAGCAAGT GTTGACTTAC CAGAAGAAAA GACTCCAATT
    TCTGATGATC CTCCTGATAC TCAAGAAATT CATGTAATTG AACATGAAAA GCTTGAAGGT
    CAAGATTCAG GACAAGAGGC TAGGAATCTT TCATTTAATG AGTTGTATCC CTCAGGAACT
    CTTAAGCTTC AATACAATTT TGATACCATT GAGCAACAGT TTTGTGACTT ACCTGATAAT
    AAAGACTCTG CTGAGTGTGA CATTGCTGAG GTGGATGGAG AACATTTTGT GGCACAGAGC
    AACTTTACCT TGATTTTGGA AGGTGAAGAA GGAGAAGTAG AGACAGATGA TTCTGCAGCA
    TCTGATGTGT TAGCTAAAGC AGCTAATGCA GCCACTGAGG AAAAACATGT ATGCAGTGAG
    GAAAGTGAGA ATCACAGGCA TGTTGCAGAT TTGCCATCTG TCATAATGAG TGATCAGAAG
    TCCCAGAGGG TAGAGACATT ACCATATGTG CCTGAACCAA TTAAAGTAGC AATTGCAGAA
    AATTTATTAG ATGTAATTAA AGACACAAGA AGTAAAGAAA TTACTTCAGA TACAATGGAA
    CAGTCCATTC AAGAAAACAT ACCTTTAATA AGCCAAAAGG TAAAGTGTTC CACCAAGTTA
    GCCAACTTTA CAACTAAGAC TGTTCAGGAA ACAAGTACAG AGACTATAAA TGTCAGCGAG
    GTTAATGACA TGATTTCCTC CAGAACTCGC AAGAGAGGAC GTGTCCAAAA CCTGAATGTC
    AAATCAGCAC AACAGGAAGA GCCAGCCGCT GTTGCTACTC CTGAGATGCT GGGGCTTTCA
    GTTACAAAGA AAACTAGGAA AACAAAAGAA ATTGCAGGGG CTCTGGAAAG TGCCAGTTCT
    GATGACAAGG GAGTATCTCA TAACCAGCAA ATACCTCAAA ATGCTATTAC TCCTAGGAGA
    GGAAGGAGAA AAAAAGATGT TAATCAAGAC ACATTAGAAA ATGCTAATTC TGTGGAACAA
    GAATTACAAG TTACTCCAGG TAGGGAATTA AGAAGGTTAA AATCATCTCA GCTGTTGGAA
    GCAGCAACTG AAGAAAGTTC TTTTAGAAAA GAAGTTAAGC TTTCATCTGC TACAAAAAGG
    ACTCCTAGAA GAACTAAATC TGTAGAAAAT CGGGAAAGTG TTGAAGTTAT AAATGATCTG
    AAGGTCAGCA AAGTAGCAGG TCCTAGCAGA AGTACCAGAA AATTGAGAAG TACTCATTTG
    GAAGCTTCTG AAAGTGTAGG ATATAAACAA GAGGGGGAGT CCGCTGAACA ACAGCTGCCT
    ATTCAACGTG GTAAAAGGGT TAAAAAGAAA GAAGTTAGTG CGTTGGATGT TACAGAAGAT
    TCCAAACTTG ATTCCTCCCA GTTGACTATT CAAGCAGATT TTGGTACACC TGCTACACCT
    AGGAAACGTG GTAGACCAAG AAAAATCAAT CCATCTGAAG ATGTAGAATC TAAGGCTGTT
    ACAGAAGCAG GAAGTCTCAA GAAGAGAGAA GGTCTCAGCA TCCAAAGGAG ATCCACAAGA
    AACACCCCAG CTAAAAGTGA AAATACTGAT GTTGGGAAAC CAACTTTAGA AGAGTCAGTT
    TTCATGCCAA ATGAGGAAGT TGCTGCAGTG ATGAGCTCTA AGAAGCAGCT TACAAAAAGG
    ACTGAAAACC AAAGCCAGAA ACGTTCATTA CGTTCAATAC CAGAAAAGTG CATAGATGAA
    GAAACGGCAC ACACAGAGCC AGATGCCCAA GAAGAAAGAT TGCTTGCTAC AGTTGCTTTG
    ACTAAATCTT CCCGTAGCAC ACGGACTAGA TCTACCAAGG CCATCTTGTT GCCGGACCTT
    TCTGAACCAA AAAATGAGTC TCTTTTATTT TCTCCTCCTG TGTCAAAGAT TCCAAGGAAA
    GAGAAAGCTA AAAAAATAGA GGCTCCTGCA CAGCTGCAAG AATTAGTTTC AGACTTACCT
    TCTCAATTTG TCTCCTCACC TCCTGCTTTA AGGACCAGGC GGAAAAACCC ATTGAATACA
    TCCAAACATA CAGATGGACT GGAAGATGAT GGTCAAACAA CTGAAACTGT GGGAAAGCAA
    CAAGTGAAAA GAATCAGGAC TGCAAAAACA AAACAAGCAA GCAAAAACAC AGAAAAAGAA
    AGTTGGTCAC CTCCCCCGGT AGAAATTAAG CTGATTTCTC CCTTGGCAAG CCCAGTTGAT
    GGAGTCAAGA GCAAACCAAG AAAGACTGCA GAAGTAACGG GAAAAACTGT TGGAAGGAGC
    AGGAAGAAGT TGTCTTCTTT TCCAAAGCAA GTTCTACGCA GAAAAATGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223471
    Clone ID Related Accession (Same CDS sequence) NM_001192036.2 , XM_005216798.4
    Accession Version XM_005216798.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216798.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CAGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC AGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGCAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216855.2
    CDS426..7256
    Translation

    Target ORF information:

    RefSeq Version XM_005216798.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216798.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CAGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC AGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGCAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223472
    Clone ID Related Accession (Same CDS sequence) XM_024976089.1
    Accession Version XM_024976089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGCCACGCCC
    GAAGTCCCTG GTGATAGTGG AAGATCTGGG CCTGTTACCT CTGAAAGCCC TGTAGTCTCT
    GAACATAGGC TTGACCAGGA AGTAGAGTTG AACTTAAAAG AAGATCATGA AATAGAAGTT
    GATGTACTAA AAGCAAGTGT TGACTTACCA GAAGAAAAGA CTCCAATTTC TGATGATCCT
    CCTGATACTC AAGAAATTCA TGTAATTGAA CATGAAAAGC TTGAAGGTCA AGATTCAGGA
    CAAGAGGCTA GGAATCTTTC ATTTAATGAG TTGTATCCCT CAGGAACTCT TAAGCTTCAA
    TACAATTTTG ATACCATTGA GCAACAGTTT TGTGACTTAC CTGATAATAA AGACTCTGCT
    GAGTGTGACA TTGCTGAGGT GGATGGAGAA CATTTTGTGG CACAGAGCAA CTTTACCTTG
    ATTTTGGAAG GTGAAGAAGG AGAAGTAGAG ACAGATGATT CTGCAGCATC TGATGTGTTA
    GCTAAAGCAG CTAATGCAGC CACTGAGGAA AAACATGTAT GCAGTGAGGA AAGTGAGAAT
    CACAGGCATG TTGCAGATTT GCCATCTGTC ATAATGAGTG ATCAGAAGTC CCAGAGGGTA
    GAGACATTAC CATATGTGCC TGAACCAATT AAAGTAGCAA TTGCAGAAAA TTTATTAGAT
    GTAATTAAAG ACACAAGAAG TAAAGAAATT ACTTCAGATA CAATGGAACA GTCCATTCAA
    GAAAACATAC CTTTAATAAG CCAAAAGGTA AAGTGTTCCA CCAAGTTAGC CAACTTTACA
    ACTAAGACTG TTCAGGAAAC AAGTACAGAG ACTATAAATG TCAGCGAGGT TAATGACATG
    ATTTCCTCCA GAACTCGCAA GAGAGGACGT GTCCAAAACC TGAATGTCAA ATCAGCACAA
    CAGGAAGAGC CAGCCGCTGT TGCTACTCCT GAGATGCTGG GGCTTTCAGT TACAAAGAAA
    ACTAGGAAAA CAAAAGAAAT TGCAGGGGCT CTGGAAAGTG CCAGTTCTGA TGACAAGGGA
    GTATCTCATA ACCAGCAAAT ACCTCAAAAT GCTATTACTC CTAGGAGAGG AAGGAGAAAA
    AAAGATGTTA ATCAAGACAC ATTAGAAAAT GCTAATTCTG TGGAACAAGA ATTACAAGTT
    ACTCCAGGTA GGGAATTAAG AAGGTTAAAA TCATCTCAGC TGTTGGAAGC AGCAACTGAA
    GAAAGTTCTT TTAGAAAAGA AGTTAAGCTT TCATCTGCTA CAAAAAGGAC TCCTAGAAGA
    ACTAAATCTG TAGAAAATCG GGAAAGTGTT GAAGTTATAA ATGATCTGAA GGTCAGCAAA
    GTAGCAGGTC CTAGCAGAAG TACCAGAAAA TTGAGAAGTA CTCATTTGGA AGCTTCTGAA
    AGTGTAGGAT ATAAACAAGA GGGGGAGTCC GCTGAACAAC AGCTGCCTAT TCAACGTGGT
    AAAAGGGTTA AAAAGAAAGA AGTTAGTGCG TTGGATGTTA CAGAAGATTC CAAACTTGAT
    TCCTCCCAGT TGACTATTCA AGCAGATTTT GGTACACCTG CTACACCTAG GAAACGTGGT
    AGACCAAGAA AAATCAATCC ATCTGAAGAT GTAGAATCTA AGGCTGTTAC AGAAGCAGGA
    AGTCTCAAGA AGAGAGAAGG TCTCAGCATC CAAAGGAGAT CCACAAGAAA CACCCCAGCT
    AAAAGTGAAA ATACTGATGT TGGGAAACCA ACTTTAGAAG AGTCAGTTTT CATGCCAAAT
    GAGGAAGTTG CTGCAGTGAT GAGCTCTAAG AAGCAGCTTA CAAAAAGGAC TGAAAACCAA
    AGCCAGAAAC GTTCATTACG TTCAATACCA GAAAAGTGCA TAGATGAAGA AACGGCACAC
    ACAGAGCCAG ATGCCCAAGA AGAAAGATTG CTTGCTACAG TTGCTTTGAC TAAATCTTCC
    CGTAGCACAC GGACTAGATC TACCAAGGCC ATCTTGTTGC CGGACCTTTC TGAACCAAAA
    AATGAGTCTC TTTTATTTTC TCCTCCTGTG TCAAAGATTC CAAGGAAAGA GAAAGCTAAA
    AAAATAGAGG CTCCTGCACA GCTGCAAGAA TTAGTTTCAG ACTTACCTTC TCAATTTGTC
    TCCTCACCTC CTGCTTTAAG GACCAGGCGG AAAAACCCAT TGAATACATC CAAACATACA
    GATGGACTGG AAGATGATGG TCAAACAACT GAAACTGTGG GAAAGCAACA AGTGAAAAGA
    ATCAGGACTG CAAAAACAAA ACAAGCAAGC AAAAACACAG AAAAAGAAAG TTGGTCACCT
    CCCCCGGTAG AAATTAAGCT GATTTCTCCC TTGGCAAGCC CAGTTGATGG AGTCAAGAGC
    AAACCAAGAA AGACTGCAGA AGTAACGGGA AAAACTGTTG GAAGGAGCAG GAAGAAGTTG
    TCTTCTTTTC CAAAGCAAGT TCTACGCAGA AAAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831857.1
    CDS142..6963
    Translation

    Target ORF information:

    RefSeq Version XM_024976089.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAAAGGGAA CTCCTCAGCC TGAAGTCCAC AGTACCTGTT
    TTAGACGAAG GACTGGTCTC TGTTGAAGCC TATACCTCTG CAGTTACAGC AGCCACGCCC
    GAAGTCCCTG GTGATAGTGG AAGATCTGGG CCTGTTACCT CTGAAAGCCC TGTAGTCTCT
    GAACATAGGC TTGACCAGGA AGTAGAGTTG AACTTAAAAG AAGATCATGA AATAGAAGTT
    GATGTACTAA AAGCAAGTGT TGACTTACCA GAAGAAAAGA CTCCAATTTC TGATGATCCT
    CCTGATACTC AAGAAATTCA TGTAATTGAA CATGAAAAGC TTGAAGGTCA AGATTCAGGA
    CAAGAGGCTA GGAATCTTTC ATTTAATGAG TTGTATCCCT CAGGAACTCT TAAGCTTCAA
    TACAATTTTG ATACCATTGA GCAACAGTTT TGTGACTTAC CTGATAATAA AGACTCTGCT
    GAGTGTGACA TTGCTGAGGT GGATGGAGAA CATTTTGTGG CACAGAGCAA CTTTACCTTG
    ATTTTGGAAG GTGAAGAAGG AGAAGTAGAG ACAGATGATT CTGCAGCATC TGATGTGTTA
    GCTAAAGCAG CTAATGCAGC CACTGAGGAA AAACATGTAT GCAGTGAGGA AAGTGAGAAT
    CACAGGCATG TTGCAGATTT GCCATCTGTC ATAATGAGTG ATCAGAAGTC CCAGAGGGTA
    GAGACATTAC CATATGTGCC TGAACCAATT AAAGTAGCAA TTGCAGAAAA TTTATTAGAT
    GTAATTAAAG ACACAAGAAG TAAAGAAATT ACTTCAGATA CAATGGAACA GTCCATTCAA
    GAAAACATAC CTTTAATAAG CCAAAAGGTA AAGTGTTCCA CCAAGTTAGC CAACTTTACA
    ACTAAGACTG TTCAGGAAAC AAGTACAGAG ACTATAAATG TCAGCGAGGT TAATGACATG
    ATTTCCTCCA GAACTCGCAA GAGAGGACGT GTCCAAAACC TGAATGTCAA ATCAGCACAA
    CAGGAAGAGC CAGCCGCTGT TGCTACTCCT GAGATGCTGG GGCTTTCAGT TACAAAGAAA
    ACTAGGAAAA CAAAAGAAAT TGCAGGGGCT CTGGAAAGTG CCAGTTCTGA TGACAAGGGA
    GTATCTCATA ACCAGCAAAT ACCTCAAAAT GCTATTACTC CTAGGAGAGG AAGGAGAAAA
    AAAGATGTTA ATCAAGACAC ATTAGAAAAT GCTAATTCTG TGGAACAAGA ATTACAAGTT
    ACTCCAGGTA GGGAATTAAG AAGGTTAAAA TCATCTCAGC TGTTGGAAGC AGCAACTGAA
    GAAAGTTCTT TTAGAAAAGA AGTTAAGCTT TCATCTGCTA CAAAAAGGAC TCCTAGAAGA
    ACTAAATCTG TAGAAAATCG GGAAAGTGTT GAAGTTATAA ATGATCTGAA GGTCAGCAAA
    GTAGCAGGTC CTAGCAGAAG TACCAGAAAA TTGAGAAGTA CTCATTTGGA AGCTTCTGAA
    AGTGTAGGAT ATAAACAAGA GGGGGAGTCC GCTGAACAAC AGCTGCCTAT TCAACGTGGT
    AAAAGGGTTA AAAAGAAAGA AGTTAGTGCG TTGGATGTTA CAGAAGATTC CAAACTTGAT
    TCCTCCCAGT TGACTATTCA AGCAGATTTT GGTACACCTG CTACACCTAG GAAACGTGGT
    AGACCAAGAA AAATCAATCC ATCTGAAGAT GTAGAATCTA AGGCTGTTAC AGAAGCAGGA
    AGTCTCAAGA AGAGAGAAGG TCTCAGCATC CAAAGGAGAT CCACAAGAAA CACCCCAGCT
    AAAAGTGAAA ATACTGATGT TGGGAAACCA ACTTTAGAAG AGTCAGTTTT CATGCCAAAT
    GAGGAAGTTG CTGCAGTGAT GAGCTCTAAG AAGCAGCTTA CAAAAAGGAC TGAAAACCAA
    AGCCAGAAAC GTTCATTACG TTCAATACCA GAAAAGTGCA TAGATGAAGA AACGGCACAC
    ACAGAGCCAG ATGCCCAAGA AGAAAGATTG CTTGCTACAG TTGCTTTGAC TAAATCTTCC
    CGTAGCACAC GGACTAGATC TACCAAGGCC ATCTTGTTGC CGGACCTTTC TGAACCAAAA
    AATGAGTCTC TTTTATTTTC TCCTCCTGTG TCAAAGATTC CAAGGAAAGA GAAAGCTAAA
    AAAATAGAGG CTCCTGCACA GCTGCAAGAA TTAGTTTCAG ACTTACCTTC TCAATTTGTC
    TCCTCACCTC CTGCTTTAAG GACCAGGCGG AAAAACCCAT TGAATACATC CAAACATACA
    GATGGACTGG AAGATGATGG TCAAACAACT GAAACTGTGG GAAAGCAACA AGTGAAAAGA
    ATCAGGACTG CAAAAACAAA ACAAGCAAGC AAAAACACAG AAAAAGAAAG TTGGTCACCT
    CCCCCGGTAG AAATTAAGCT GATTTCTCCC TTGGCAAGCC CAGTTGATGG AGTCAAGAGC
    AAACCAAGAA AGACTGCAGA AGTAACGGGA AAAACTGTTG GAAGGAGCAG GAAGAAGTTG
    TCTTCTTTTC CAAAGCAAGT TCTACGCAGA AAAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223473
    Clone ID Related Accession (Same CDS sequence) XM_024976090.1
    Accession Version XM_024976090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGC CACGCCCGAA
    GTCCCTGGTG ATAGTGGAAG ATCTGGGCCT GTTACCTCTG AAAGCCCTGT AGTCTCTGAA
    CATAGGCTTG ACCAGGAAGT AGAGTTGAAC TTAAAAGAAG ATCATGAAAT AGAAGTTGAT
    GTACTAAAAG CAAGTGTTGA CTTACCAGAA GAAAAGACTC CAATTTCTGA TGATCCTCCT
    GATACTCAAG AAATTCATGT AATTGAACAT GAAAAGCTTG AAGGTCAAGA TTCAGGACAA
    GAGGCTAGGA ATCTTTCATT TAATGAGTTG TATCCCTCAG GAACTCTTAA GCTTCAATAC
    AATTTTGATA CCATTGAGCA ACAGTTTTGT GACTTACCTG ATAATAAAGA CTCTGCTGAG
    TGTGACATTG CTGAGGTGGA TGGAGAACAT TTTGTGGCAC AGAGCAACTT TACCTTGATT
    TTGGAAGGTG AAGAAGGAGA AGTAGAGACA GATGATTCTG CAGCATCTGA TGTGTTAGCT
    AAAGCAGCTA ATGCAGCCAC TGAGGAAAAA CATGTATGCA GTGAGGAAAG TGAGAATCAC
    AGGCATGTTG CAGATTTGCC ATCTGTCATA ATGAGTGATC AGAAGTCCCA GAGGGTAGAG
    ACATTACCAT ATGTGCCTGA ACCAATTAAA GTAGCAATTG CAGAAAATTT ATTAGATGTA
    ATTAAAGACA CAAGAAGTAA AGAAATTACT TCAGATACAA TGGAACAGTC CATTCAAGAA
    AACATACCTT TAATAAGCCA AAAGGTAAAG TGTTCCACCA AGTTAGCCAA CTTTACAACT
    AAGACTGTTC AGGAAACAAG TACAGAGACT ATAAATGTCA GCGAGGTTAA TGACATGATT
    TCCTCCAGAA CTCGCAAGAG AGGACGTGTC CAAAACCTGA ATGTCAAATC AGCACAACAG
    GAAGAGCCAG CCGCTGTTGC TACTCCTGAG ATGCTGGGGC TTTCAGTTAC AAAGAAAACT
    AGGAAAACAA AAGAAATTGC AGGGGCTCTG GAAAGTGCCA GTTCTGATGA CAAGGGAGTA
    TCTCATAACC AGCAAATACC TCAAAATGCT ATTACTCCTA GGAGAGGAAG GAGAAAAAAA
    GATGTTAATC AAGACACATT AGAAAATGCT AATTCTGTGG AACAAGAATT ACAAGTTACT
    CCAGGTAGGG AATTAAGAAG GTTAAAATCA TCTCAGCTGT TGGAAGCAGC AACTGAAGAA
    AGTTCTTTTA GAAAAGAAGT TAAGCTTTCA TCTGCTACAA AAAGGACTCC TAGAAGAACT
    AAATCTGTAG AAAATCGGGA AAGTGTTGAA GTTATAAATG ATCTGAAGGT CAGCAAAGTA
    GCAGGTCCTA GCAGAAGTAC CAGAAAATTG AGAAGTACTC ATTTGGAAGC TTCTGAAAGT
    GTAGGATATA AACAAGAGGG GGAGTCCGCT GAACAACAGC TGCCTATTCA ACGTGGTAAA
    AGGGTTAAAA AGAAAGAAGT TAGTGCGTTG GATGTTACAG AAGATTCCAA ACTTGATTCC
    TCCCAGTTGA CTATTCAAGC AGATTTTGGT ACACCTGCTA CACCTAGGAA ACGTGGTAGA
    CCAAGAAAAA TCAATCCATC TGAAGATGTA GAATCTAAGG CTGTTACAGA AGCAGGAAGT
    CTCAAGAAGA GAGAAGGTCT CAGCATCCAA AGGAGATCCA CAAGAAACAC CCCAGCTAAA
    AGTGAAAATA CTGATGTTGG GAAACCAACT TTAGAAGAGT CAGTTTTCAT GCCAAATGAG
    GAAGTTGCTG CAGTGATGAG CTCTAAGAAG CAGCTTACAA AAAGGACTGA AAACCAAAGC
    CAGAAACGTT CATTACGTTC AATACCAGAA AAGTGCATAG ATGAAGAAAC GGCACACACA
    GAGCCAGATG CCCAAGAAGA AAGATTGCTT GCTACAGTTG CTTTGACTAA ATCTTCCCGT
    AGCACACGGA CTAGATCTAC CAAGGCCATC TTGTTGCCGG ACCTTTCTGA ACCAAAAAAT
    GAGTCTCTTT TATTTTCTCC TCCTGTGTCA AAGATTCCAA GGAAAGAGAA AGCTAAAAAA
    ATAGAGGCTC CTGCACAGCT GCAAGAATTA GTTTCAGACT TACCTTCTCA ATTTGTCTCC
    TCACCTCCTG CTTTAAGGAC CAGGCGGAAA AACCCATTGA ATACATCCAA ACATACAGAT
    GGACTGGAAG ATGATGGTCA AACAACTGAA ACTGTGGGAA AGCAACAAGT GAAAAGAATC
    AGGACTGCAA AAACAAAACA AGCAAGCAAA AACACAGAAA AAGAAAGTTG GTCACCTCCC
    CCGGTAGAAA TTAAGCTGAT TTCTCCCTTG GCAAGCCCAG TTGATGGAGT CAAGAGCAAA
    CCAAGAAAGA CTGCAGAAGT AACGGGAAAA ACTGTTGGAA GGAGCAGGAA GAAGTTGTCT
    TCTTTTCCAA AGCAAGTTCT ACGCAGAAAA ATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831858.1
    CDS142..6960
    Translation

    Target ORF information:

    RefSeq Version XM_024976090.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTTCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGC CACGCCCGAA
    GTCCCTGGTG ATAGTGGAAG ATCTGGGCCT GTTACCTCTG AAAGCCCTGT AGTCTCTGAA
    CATAGGCTTG ACCAGGAAGT AGAGTTGAAC TTAAAAGAAG ATCATGAAAT AGAAGTTGAT
    GTACTAAAAG CAAGTGTTGA CTTACCAGAA GAAAAGACTC CAATTTCTGA TGATCCTCCT
    GATACTCAAG AAATTCATGT AATTGAACAT GAAAAGCTTG AAGGTCAAGA TTCAGGACAA
    GAGGCTAGGA ATCTTTCATT TAATGAGTTG TATCCCTCAG GAACTCTTAA GCTTCAATAC
    AATTTTGATA CCATTGAGCA ACAGTTTTGT GACTTACCTG ATAATAAAGA CTCTGCTGAG
    TGTGACATTG CTGAGGTGGA TGGAGAACAT TTTGTGGCAC AGAGCAACTT TACCTTGATT
    TTGGAAGGTG AAGAAGGAGA AGTAGAGACA GATGATTCTG CAGCATCTGA TGTGTTAGCT
    AAAGCAGCTA ATGCAGCCAC TGAGGAAAAA CATGTATGCA GTGAGGAAAG TGAGAATCAC
    AGGCATGTTG CAGATTTGCC ATCTGTCATA ATGAGTGATC AGAAGTCCCA GAGGGTAGAG
    ACATTACCAT ATGTGCCTGA ACCAATTAAA GTAGCAATTG CAGAAAATTT ATTAGATGTA
    ATTAAAGACA CAAGAAGTAA AGAAATTACT TCAGATACAA TGGAACAGTC CATTCAAGAA
    AACATACCTT TAATAAGCCA AAAGGTAAAG TGTTCCACCA AGTTAGCCAA CTTTACAACT
    AAGACTGTTC AGGAAACAAG TACAGAGACT ATAAATGTCA GCGAGGTTAA TGACATGATT
    TCCTCCAGAA CTCGCAAGAG AGGACGTGTC CAAAACCTGA ATGTCAAATC AGCACAACAG
    GAAGAGCCAG CCGCTGTTGC TACTCCTGAG ATGCTGGGGC TTTCAGTTAC AAAGAAAACT
    AGGAAAACAA AAGAAATTGC AGGGGCTCTG GAAAGTGCCA GTTCTGATGA CAAGGGAGTA
    TCTCATAACC AGCAAATACC TCAAAATGCT ATTACTCCTA GGAGAGGAAG GAGAAAAAAA
    GATGTTAATC AAGACACATT AGAAAATGCT AATTCTGTGG AACAAGAATT ACAAGTTACT
    CCAGGTAGGG AATTAAGAAG GTTAAAATCA TCTCAGCTGT TGGAAGCAGC AACTGAAGAA
    AGTTCTTTTA GAAAAGAAGT TAAGCTTTCA TCTGCTACAA AAAGGACTCC TAGAAGAACT
    AAATCTGTAG AAAATCGGGA AAGTGTTGAA GTTATAAATG ATCTGAAGGT CAGCAAAGTA
    GCAGGTCCTA GCAGAAGTAC CAGAAAATTG AGAAGTACTC ATTTGGAAGC TTCTGAAAGT
    GTAGGATATA AACAAGAGGG GGAGTCCGCT GAACAACAGC TGCCTATTCA ACGTGGTAAA
    AGGGTTAAAA AGAAAGAAGT TAGTGCGTTG GATGTTACAG AAGATTCCAA ACTTGATTCC
    TCCCAGTTGA CTATTCAAGC AGATTTTGGT ACACCTGCTA CACCTAGGAA ACGTGGTAGA
    CCAAGAAAAA TCAATCCATC TGAAGATGTA GAATCTAAGG CTGTTACAGA AGCAGGAAGT
    CTCAAGAAGA GAGAAGGTCT CAGCATCCAA AGGAGATCCA CAAGAAACAC CCCAGCTAAA
    AGTGAAAATA CTGATGTTGG GAAACCAACT TTAGAAGAGT CAGTTTTCAT GCCAAATGAG
    GAAGTTGCTG CAGTGATGAG CTCTAAGAAG CAGCTTACAA AAAGGACTGA AAACCAAAGC
    CAGAAACGTT CATTACGTTC AATACCAGAA AAGTGCATAG ATGAAGAAAC GGCACACACA
    GAGCCAGATG CCCAAGAAGA AAGATTGCTT GCTACAGTTG CTTTGACTAA ATCTTCCCGT
    AGCACACGGA CTAGATCTAC CAAGGCCATC TTGTTGCCGG ACCTTTCTGA ACCAAAAAAT
    GAGTCTCTTT TATTTTCTCC TCCTGTGTCA AAGATTCCAA GGAAAGAGAA AGCTAAAAAA
    ATAGAGGCTC CTGCACAGCT GCAAGAATTA GTTTCAGACT TACCTTCTCA ATTTGTCTCC
    TCACCTCCTG CTTTAAGGAC CAGGCGGAAA AACCCATTGA ATACATCCAA ACATACAGAT
    GGACTGGAAG ATGATGGTCA AACAACTGAA ACTGTGGGAA AGCAACAAGT GAAAAGAATC
    AGGACTGCAA AAACAAAACA AGCAAGCAAA AACACAGAAA AAGAAAGTTG GTCACCTCCC
    CCGGTAGAAA TTAAGCTGAT TTCTCCCTTG GCAAGCCCAG TTGATGGAGT CAAGAGCAAA
    CCAAGAAAGA CTGCAGAAGT AACGGGAAAA ACTGTTGGAA GGAGCAGGAA GAAGTTGTCT
    TCTTTTCCAA AGCAAGTTCT ACGCAGAAAA ATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27067
    Clone ID Related Accession (Same CDS sequence) XM_005216798.3 , NM_001192036.1
    Accession Version NM_001192036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product protein ELYS
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02042685.1 and DAAA02042686.1. On Jul 17, 2010 this sequence version replaced XM_580880.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403639, SAMN02403640 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTCCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CGGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC GGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGAAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178965.1
    CDS1..6831
    Translation

    Target ORF information:

    RefSeq Version NM_001192036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTCCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CGGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC GGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGAAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27067
    Clone ID Related Accession (Same CDS sequence) XM_005216798.3 , NM_001192036.1
    Accession Version XM_005216798.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ELYS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104436.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216798.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTCCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CGGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC GGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGAAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216855.1
    CDS392..7222
    Translation

    Target ORF information:

    RefSeq Version XM_005216798.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AT-hook containing transcription factor 1 (AHCTF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216798.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCGAGACC TAACGGCGCA GGTGACAAGT GGTCTTCTGC AGTTCCCAGA AGTGACTATC 
    CAAGCCCTTG GAGAAGACGA AATAACATTA GAATCTGTGC TTCGTGGAAA GTTTGCTGCA
    GGGAAAAATG GACTTGCTTG CTTGGCCTGT GGTCCACAAC TTGAGGTAGT GAATTCTCTA
    ACAGGAGAGC GGTTGTCTGC ATATAGATTC AGTGGAGTAA ATGAACAGCC TCCTATAGTC
    CTCGCTGTGA AAGAATTCTC TTGGCAGAAG AGAACTGGAT TGTTAATTGG ATTGGAAGAA
    GTAGAAGGGA GTGTTCTCTG TCTTTATGAC CTTGGTTTAT CAAGAGTGGT TAAAGCAGTT
    GTTCTTCCTG GACGGGTAAC AGCTATTGAA CCTATAATTA ATCATGGAGG AGCCAGCGCA
    AGCACTCAGC ATTTACACCC AAGTCTGCGA TGGCTTTTTG GTGTGGCAGC TGTGGTAACT
    GATGTTGGAC AGATCCTTCT TATTGATCTA TGTTTGGATG ACTTGTCATG CAGTCAGAAT
    GAAGTAGAAG CATCAGACCT TGAAGTTATA ACTGGCATCC CAGCTGAAGT ACCGCGCATC
    AGAGAAAATG TGATGCGAGA AGGACGCCAT CTGTGTTTTC AGTTAGTCAG CCCATCAGGA
    ACAGCCGTTT CAACCCTTAG TTACATAAAC CGGACAAATC AGCTTGCTGT CGGTTTTTCT
    GATGGCTATC TAGCACTTTG GAACATGAAA AGCATGAAAA AAGAATACTA CACACAGTTG
    GAAGGTGGAA GAGTTCCTGT GTATGCTGTC ACTTTTCAAG AACCTGAAAA TGATCCTCGT
    AATTGCTGTT ACCTCTGGGC TGTTCAGTCT ACACAAGATA GTGAAGGGGA TGTTTTGAGT
    TTACATCTGC TTCAGCTGGC CTTTGGTGAC AGAAAGTGTT TGGCGTCAGG ACAAATCTTA
    TATGAGGGGT TGGAGTACTG TGAAGAAAGA TACACACTGG ACCTCACAGG TGGCATGTTT
    CCTTTGAGGG GACAGACTGG TAATACCAAA CTTTTGGGAT GCCAGAGTAT AGAGAAATTT
    CGATCTCACG GTGACAGGGA GGAAAGCATG AATGAAGCTC TGTCTCCTGA CACCAGTGTT
    TCAGTCTTTA CCTGGCAGGT GAATATATAT GGACAGGGAA AGCCTTCTAT ATATTTGGGG
    CTTTTCGACA TAAATCGTTG GTACCATGCA CAAATGCCAG ATTCGTTAAG ATCAGGAGAA
    TATCTGCATA ACTGCTCTTA TTTTGCGTTG TGGTCACTGG ATGCTGTTGT AAGTAGGACG
    TCTCCACATT ACATCTTGGA TATATTAGTA CATGAGAGAA GTTTAAGTCG AGGAGTTCCT
    CCCTCATATC CACCTCCTGA GCAGTTTTTT AACCCAAGTA CTTACAATTT TGATGCCACT
    TGTTTGTTAA ACTCAGGAGT TGTTCATATA ACTTGTACTG GCTTTCAAAA GGAGACTTTG
    ACTTTTTTAA AGAAATCAGG ACCATCTCTC AATGAAGTCA TTCCTGATGG TTATAATCGA
    TGTCTTGTGG CTGGCCTTCT CTCACCAAGA CTTATTGATA TTCAACCTTC CAGTTTAAGT
    CAGGAAGAAC AGTTAGAAGC TATATTGTCA GCAGCAATCC AGACAAGTTC TCTGGGACTT
    TTGACAGGGT GTATCAAAAG ATGGATAACA GAAGAACAAC CAAATTCTGC TGCTAATTTA
    CGCTTTGTTC TTGAATGGTC ATGGAATAAA GTGGTTTTCA CAAAAGAGGA ATTTGACAGA
    CTATGTACAC CGTTATTTGA TGGTTCATGT CGTTTCATCG ACCCACAAAC TATACAGTCT
    CTCCAGCAGT GCCATTTACT TCTTAGCAAT CTTAGTACAG TCTTAAGCTG TTTTGCAGCT
    GAGGCCCACG ACATCACTGA GAGAGGTCTT ACAGATTTAA GCAATAAATA TATGGTTACT
    CAGCTCATCT GTCAGTATGC ACAAGTGGTT CTTTGGTTCT CTCATTCTGG CCTTTTACCA
    GAGGGCTTAG ATGATGCTGT GCAGTTGTCA AGGTTATGCT ACAACTACCC CGTAATTCAG
    AATTACTACA CCAGTCGTCG ACAGAAGTGT GAGCGTTCAT CAAGAGGGAA GTGGAACCCT
    GACTGCTTGA TGATTGATGG AATGGTTTCT CAGTTAGGAG ATCGAGTAGA GAAGTTGTGG
    AAACGGGACG AAGGAGGCAC AGGAAAATAT CCTCCTACGA GTCTACATGC GTTACTTGAC
    ATATACCTAC TAGACAACAT TACTGAAGCA AGCAAGCATT CTGTTACTAT TTATTTGCTA
    CTTGATATTA TGTATTCCTT TCCAAACAAA ACGGATACTT CCATTGAATC ATTTCCAACT
    GCCTTTGCCA TTTCTTGGGG CCAGGTTAAA CTTATTCAAG GATTTTGGCT GATAGATCAT
    AATGACTATG AGAGTGGTTT GGATCTTCTG TTTCACCCGG CTACTGCAAA ACCTGTGTCA
    TGGCAACATT CAAAGATTAT TGAAGCCTTC ATGAGTCAGG GTGAGCACAG ACAGGCCCTC
    AGATATATTC AGACAATGAA GCCAACAGTG TCCAGTGGTA GAGATGTTAT CCTTCACCTC
    ACTGTTTTGC TTTTTAATAG GTGCATGGTG GAGGCCTGGA CTTTACTGCG ACAACATTCT
    AATAGGTTGA ATATAGAGGA GCTGCTCAAG CATACATATG AAGTCTGTCA GGAGATGGGC
    TTAATGGAGG ATTTACTGAA GCTACCATTC ACAGACACTG AGCAGGAGTG TTTGGTGAGA
    TTTTTACAGT CCAGTGCGAG TGTTCAGAAT CATGAATTCC TTTTAGTTCA CCATTTGCAG
    CGTGCCAATT ATATTCCTGC CTTGAAGCTG AACCAGACTC TGAAGATTAA TCTTATGAAT
    GATCGTGATC CTCGTTTGCG CGAGAGATCA GTGACTCGAA ATTCTATAAT AGACCAATAT
    GGGAAAATTC TTCCTAGAGT CCAGCGAAAA TTAGCCATTG AGCGAGCAAA GCCTTACCAT
    CTGTCAGCAT CATCAGTTTT TCGAGAAGTT TCTAGACCAA AACCATTATC AGCAGTTCCA
    AAGCAGGCTG TAACTGGAAC TGTGTTAACA AGATCTACTT TCATCAATAA TGTGTTATCT
    AAAATTGGAG AGGTCTGGGC AAGTAATGAA CCCGAAAATA GTATCTCTGT CTACAATAGT
    CCTAAAGTAG AAGAACCCTC TCCTGTAGCA TATTCTCTCC CAGATCCAGA GCTGCCTGAG
    GCATTTGTTG GAACACCAAT TACAAAGGCA TCACAAAGAA TTTCTAGACT GCTGGATTTG
    GTTGTTCATC CTGTCCCCCA GCCCTCTCAG TGTTTGGAGT TGATTCAGCA AAGCCCCACA
    AGGTCTCCTT GGTCCCTGGC ATCTAGTTCA TTGCCAGTAA GTTCACAATT AAAAGGATCA
    CGTCAGAATG CCTCCAGGGC TTCAGAATTA CATTTACTTG AAACTCCTCT TGTTGTTAAG
    AAAGCTAAGA CTTTGGCTAT GTCAGTTACT TCCTCTGGAT TTGCCGAGTT CACTCCTCAA
    TCCATCCTAA GGTCTGGTCT TCGAACAACA CCTTTAGCAT CTCCCTCTCT GTCACCTGGA
    AGATCTCTTA GTCCTCCTTT ACGACTCAAA GAAACTAGAA TTTCGTTCAT GGAAGAAGGT
    GTAACCACGA AATGGACTGC TGCTACTGTA GATGACAGCA AAGCAAAAAC ATTTATTACT
    GCATCTTTAC ATAAATGTGG GGCTCCAGCA GAAACTGAAT GGCTGAAAAA CAAAGATAAG
    ACAACATCTT TCCCCCTGGA TAGCCCTGAA GAGGAACATC AAGAAATGGA TGTCAGATCA
    CAGGATACAA CTTCACAAAG TTTGGAGAAA CTGGATGTGA GCGTAGAAAA TAGCAATACT
    TCCACCAGAT CAGACCAGAC TACCTTAGAG TATCAGGATG CTCCATTACC AGAAGACTTT
    GAAGATATTT TGATAGCCTC TAAGTCAGTG AGCTCTTCCA CTGAACTGGT TGCTAATTTA
    ACTGAACAGA CTGAGAAGAA CAGTGATAAA GATACATTTG AACCAGGAGT GACTCCTCCA
    GAGCCAGAGA AACAAATGGG CACTTTAGAA GATGCAGAAG CAAAGGATGG CTTAGTTGTG
    GAGGGTGTGC TTTCAGAATT AAATCACTTA AGCCCCATTC AAGGAGTTGA AGCTTCTCTT
    TGTGTACCAT CAGTCCGGGA AGAGGAACTC CTCAGCCTGA AGTCCACAGT ACCTGTTTTA
    GACGAAGGAC TGGTCTCTGT TGAAGCCTAT ACCTCTGCAG TTACAGCAGG TAACAATAAA
    TCCACGCCCG AAGTCCCTGG TGATAGTGGA AGATCTGGGC CTGTTACCTC TGAAAGCCCT
    GTAGTCTCTG AACATAGGCT TGACCAGGAA GTAGAGTTGA ACTTAAAAGA AGATCATGAA
    ATAGAAGTTG ATGTACTAAA AGCAAGTGTT GACTTACCAG AAGAAAAGAC TCCAATTTCT
    GATGATCCTC CTGATACTCA AGAAATTCAT GTAATTGAAC ATGAAAAGCT TGAAGGTCAA
    GATTCAGGAC AAGAGGCTAG GAATCTTTCA TTTAATGAGT TGTATCCCTC AGGAACTCTT
    AAGCTTCAAT ACAATTTTGA TACCATTGAG CAACAGTTTT GTGACTTACC TGATAATAAA
    GACTCTGCTG AGTGTGACAT TGCTGAGGTG GATGGAGAAC ATTTTGTGGC ACAGAGCAAC
    TTTACCTTGA TTTTGGAAGG TGAAGAAGGA GAAGTAGAGA CAGATGATTC TGCAGCATCT
    GATGTGTTAG CTAAAGCAGC TAATGCAGCC ACTGAGGAAA AACATGTATG CAGTGAGGAA
    AGTGAGAATC ACAGGCATGT TGCAGATTTG CCATCTGTCA TAATGAGTGA TCAGAAGTCC
    CAGAGGGTAG AGACATTACC ATATGTGCCT GAACCAATTA AAGTAGCAAT TGCAGAAAAT
    TTATTAGATG TAATTAAAGA CACAAGAAGT AAAGAAATTA CTTCAGATAC AATGGAACAG
    TCCATTCAAG AAAACATACC TTTAATAAGC CAAAAGGTAA AGTGTTCCAC CAAGTTAGCC
    AACTTTACAA CTAAGACTGT TCAGGAAACA AGTACAGAGA CTATAAATGT CGGCGAGGTT
    AATGACATGA TTTCCTCCAG AACTCGCAAG AGAGGACGTG TCCAAAACCT GAATGTCAAA
    TCAGCACAAC AGGAAGAGCC AGCCGCTGTT GCTACTCCTG AGATGCTGGG GCTTTCAGTT
    ACAAAGAAAA CTAGGAAAAC AAAAGAAATT GCAGGGGCTC TGGAAAGTGC CAGTTCTGAT
    GACAAGGGAG TATCTCATAA CCAGCAAATA CCTCAAAATG CTATTACTCC TAGGAGAGGA
    AGGAGAAAAA AAGATGTTAA TCAAGACACA TTAGAAAATG CTAATTCTGT GGAACAAGAA
    TTACAAGTTA CTCCAGGTAG GGAATTAAGA AGGTTAAAAT CATCTCAGCT GTTGGAAGCA
    GCAACTGAAG AAAGTTCTTT TAGAAAAGAA GTTAAGCTTT CATCTGCTAC AAAAAGGACT
    CCTAGAAGAA CTAAATCTGT AGAAAATCGG GAAAGTGTTG AAGTTATAAA TGATCTGAAG
    GTCAGCAAAG TAGCAGGTCC TAGCAGAAGT ACCAGAAAAT TGAGAAGTAC TCATTTGGAA
    GCTTCTGAAA GTGTAGGATA TAAACAAGAG GGGGAGTCCG CTGAACAACA GCTGCCTATT
    CAACGTGGTA AAAGGGTTAA AAAGAAAGAA GTTAGTGCGT TGGATGTTAC GGAAGATTCC
    AAACTTGATT CCTCCCAGTT GACTATTCAA GCAGATTTTG GTACACCTGC TACACCTAGG
    AAACGTGGTA GACCAAGAAA AATCAATCCA TCTGAAGATG TAGAATCTAA GGCTGTTACA
    GAAGCAGGAA GTCTCAAGAA GAGAGAAGGT CTCAGCATCC AAAGGAGATC CACAAGAAAC
    ACCCCAGCTA AAAGTGAAAA TACTGATGTT GGGAAACCAA CTTTAGAAGA GTCAGTTTTC
    ATGCCAAATG AGGAAGTTGC TGCAGTGATG AGCTCTAAGA AGAAGCTTAC AAAAAGGACT
    GAAAACCAAA GCCAGAAACG TTCATTACGT TCAATACCAG AAAAGTGCAT AGATGAAGAA
    ACGGCACACA CAGAGCCAGA TGCCCAAGAA GAAAGATTGC TTGCTACAGT TGCTTTGACT
    AAATCTTCCC GTAGCACACG GACTAGATCT ACCAAGGCCA TCTTGTTGCC GGACCTTTCT
    GAACCAAAAA ATGAGTCTCT TTTATTTTCT CCTCCTGTGT CAAAGATTCC AAGGAAAGAG
    AAAGCTAAAA AAATAGAGGC TCCTGCACAG CTGCAAGAAT TAGTTTCAGA CTTACCTTCT
    CAATTTGTCT CCTCACCTCC TGCTTTAAGG ACCAGGCGGA AAAACCCATT GAATACATCC
    AAACATACAG ATGGACTGGA AGATGATGGT CAAACAACTG AAACTGTGGG AAAGCAACAA
    GTGAAAAGAA TCAGGACTGC AAAAACAAAA CAAGCAAGCA AAAACACAGA AAAAGAAAGT
    TGGTCACCTC CCCCGGTAGA AATTAAGCTG ATTTCTCCCT TGGCAAGCCC AGTTGATGGA
    GTCAAGAGCA AACCAAGAAA GACTGCAGAA GTAACGGGAA AAACTGTTGG AAGGAGCAGG
    AAGAAGTTGT CTTCTTTTCC AAAGCAAGTT CTACGCAGAA AAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.