PNP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNP
Entrez Gene ID	493724
Full Name	purine nucleoside phosphorylase
Synonyms	NP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007818.1	NP_001007819.1	purine nucleoside phosphorylase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pnp2	NP_001116843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005945	XP_001688760.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005944	XP_315978.4
Saccharomyces cerevisiae (baker's yeast)	PNP1	NP_013310.1
Kluyveromyces lactis	KLLA0C16621g	XP_452943.1
Homo sapiens (human)	PNP	NP_000261.2
Canis lupus familiaris (dog)	PNP	XP_532617.2
Pan troglodytes (chimpanzee)	PNP	XP_001140576.1
	LOC710245	XP_001104622.1
Mus musculus (house mouse)	Pnp	NP_038660.1
Caenorhabditis elegans (roundworm)	K02D7.1	NP_499900.1
Rattus norvegicus (Norway rat)	Pnp	NP_001099501.1
Gallus gallus (chicken)	PNP	XP_003641950.2
Drosophila melanogaster (fruit fly)	CG16758	NP_001261330.1
Schizosaccharomyces pombe (fission yeast)	SPAC1805.16c	NP_593927.1
Bos taurus (cattle)	PNP	NP_001007819.1
Bos taurus (cattle)	LOC790312	XP_002690791.3
Eremothecium gossypii	AGOS_ACR198W	NP_983600.1
Xenopus tropicalis (tropical clawed frog)	pnp	NP_001006720.1

Gene Ontology
Bibliography

Related articles in PubMed

Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme.
Wielgus-Kutrowska B, Modrak-W?jcik A, Dyzma A, Breer K, Zolkiewski M, Bzowska A
Archives of biochemistry and biophysics54940-8(2014 May)

Second-sphere amino acids contribute to transition-state structure in bovine purine nucleoside phosphorylase.
Li L, Luo M, Ghanem M, Taylor EA, Schramm VL
Biochemistry47(8)2577-83(2008 Feb)

Fluorescence studies of calf spleen purine nucleoside phosphorylase (PNP) complexes with guanine and 9-deazaguanine.
Stepniak K, Zini? B, Wierzchowski J, Bzowska A
Nucleosides, nucleotides & nucleic acids26(6-7)841-7(2007)

Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase.
Stepniak K, Girstun A, Wielgus-Kutrowska B, Staro? K, Bzowska A
Nucleosides, nucleotides & nucleic acids26(6-7)855-9(2007)

Oligomeric structure of mammalian purine nucleoside phosphorylase in solution determined by analytical ultracentrifugation.
Behlke J, Koellner G, Bzowska A
Zeitschrift fur Naturforschung. C, Journal of biosciences60(11-12)927-31(2005 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the biological role of packing of the PNP monomers into a trimeric structure
Title: Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme.

The transition-state structure of triple mutant PNP is altered as a consequence of the amino acids in the second sphere from the catalytic site.
Title: Second-sphere amino acids contribute to transition-state structure in bovine purine nucleoside phosphorylase.

This result questions the involvement of the anionic forms in the reaction catalyzed by trimeric PNPs, and contradicts the hypothesis of a strong hydrogen bond formation between the enzyme Asn 243 residue and the purine N7 position.
Title: Fluorescence studies of calf spleen purine nucleoside phosphorylase (PNP) complexes with guanine and 9-deazaguanine.

PCR amplification of the calf phosphorylase from the calf spleen library, cloning, overexpression of the recombinant PNP, its enzymatic activity and interactions with typical ligands of mammalian wild type PNP are described.
Title: Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase.

alf spleen enzyme is a homotrimer and previous suggestions for dissociation of this enzyme into more active monomers, upon dilution of the enzyme or addition of phosphate, are incorrect.
Title: Oligomeric structure of mammalian purine nucleoside phosphorylase in solution determined by analytical ultracentrifugation.

The following PNP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41619	NM_001007818.1 Latest version!	Bos taurus purine nucleoside phosphorylase (PNP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNP ( NM_001007818.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001007819.1 Bos taurus purine nucleoside phosphorylase (PNP), mRNA.

Price & Availability↑

CloneID	OBa41619
Clone ID Related Accession (Same CDS sequence)	NM_001007818.1
Accession Version	NM_001007818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	purine nucleoside phosphorylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF529136.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF529136.1, BT021545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145483, SAMN05861786 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAATG GATATACATA TGAAGATTAT CAAGACACTG CAAAATGGCT TTTGTCTCAT 
ACCGAACAGC GACCTCAAGT GGCAGTGATC TGTGGTTCTG GGTTAGGAGG TCTGGTTAAC 
AAATTGACTC AAGCCCAGAC CTTTGACTAC AGTGAAATAC CAAACTTTCC AGAAAGTACA 
GTGCCAGGTC ATGCTGGTCG ACTGGTGTTT GGGATCTTGA ATGGCAGAGC CTGTGTGATG 
ATGCAGGGCA GGTTCCACAT GTATGAGGGC TATCCGTTTT GGAAGGTGAC ATTCCCAGTG 
AGGGTTTTCC GGCTTCTGGG TGTGGAGACC CTAGTGGTCA CCAACGCAGC TGGAGGGCTC 
AACCCCAACT TTGAGGTTGG CGATATCATG CTGATCCGTG ATCACATCAA CCTACCTGGT 
TTCAGTGGTG AGAACCCTCT CAGAGGGCCC AATGAGGAAA GGTTTGGAGT TCGTTTCCCT 
GCCATGTCTG ATGCCTACGA CCGGGATATG AGGCAGAAAG CTCACAGTAC CTGGAAACAA 
ATGGGGGAGC AAAGAGAGTT ACAGGAAGGC ACCTACGTGA TGTTGGGGGG TCCCAATTTT 
GAGACTGTGG CAGAGTGTCG CCTGCTGCGG AATCTAGGGG CAGATGCTGA AGGCATGAGC 
ACAGTACCAG AAGTTATAGT CGCAAGACAC TGTGGACTTC GAGTCTTTGG CTTCTCACTC 
ATCACAAACA AGGTCATCAT GGATTATGAA AGCCAGGGGA AGGCCAATCA TGAGGAAGTA 
CTAGAGGCTG GGAAGCAAGC TGCACAGAAA TTAGAGCAGT TTGTCTCCCT TCTTATGGCT 
AGCATTCCAG TGTCAGGGCA CACCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007819.1
CDS	93..962
Translation	MANGYTYEDYQDTAKWLLSHTEQRPQVAVICGSGLGGLVNKLTQAQTFDYSEIPNFPESTVPGHAGRLVFGILNGRACVMMQGRFHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAEGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASIPVSGHTG

Target ORF information:

RefSeq Version	NM_001007818.1
Organism	Bos taurus(cattle)
Definition	Bos taurus purine nucleoside phosphorylase (PNP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007818.1

ORF Insert Sequence:

ATGGCGAATG GATATACATA TGAAGATTAT CAAGACACTG CAAAATGGCT TTTGTCTCAT 
ACCGAACAGC GACCTCAAGT GGCAGTGATC TGTGGTTCTG GGTTAGGAGG TCTGGTTAAC 
AAATTGACTC AAGCCCAGAC CTTTGACTAC AGTGAAATAC CAAACTTTCC AGAAAGTACA 
GTGCCAGGTC ATGCTGGTCG ACTGGTGTTT GGGATCTTGA ATGGCAGAGC CTGTGTGATG 
ATGCAGGGCA GGTTCCACAT GTATGAGGGC TATCCGTTTT GGAAGGTGAC ATTCCCAGTG 
AGGGTTTTCC GGCTTCTGGG TGTGGAGACC CTAGTGGTCA CCAACGCAGC TGGAGGGCTC 
AACCCCAACT TTGAGGTTGG CGATATCATG CTGATCCGTG ATCACATCAA CCTACCTGGT 
TTCAGTGGTG AGAACCCTCT CAGAGGGCCC AATGAGGAAA GGTTTGGAGT TCGTTTCCCT 
GCCATGTCTG ATGCCTACGA CCGGGATATG AGGCAGAAAG CTCACAGTAC CTGGAAACAA 
ATGGGGGAGC AAAGAGAGTT ACAGGAAGGC ACCTACGTGA TGTTGGGGGG TCCCAATTTT 
GAGACTGTGG CAGAGTGTCG CCTGCTGCGG AATCTAGGGG CAGATGCTGA AGGCATGAGC 
ACAGTACCAG AAGTTATAGT CGCAAGACAC TGTGGACTTC GAGTCTTTGG CTTCTCACTC 
ATCACAAACA AGGTCATCAT GGATTATGAA AGCCAGGGGA AGGCCAATCA TGAGGAAGTA 
CTAGAGGCTG GGAAGCAAGC TGCACAGAAA TTAGAGCAGT TTGTCTCCCT TCTTATGGCT 
AGCATTCCAG TGTCAGGGCA CACCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme.
Archives of biochemistry and biophysics54940-8(2014 May)
Wielgus-Kutrowska B,Modrak-W?jcik A,Dyzma A,Breer K,Zolkiewski M,Bzowska A

Second-sphere amino acids contribute to transition-state structure in bovine purine nucleoside phosphorylase.
Biochemistry47(8)2577-83(2008 Feb)
Li L,Luo M,Ghanem M,Taylor EA,Schramm VL

Fluorescence studies of calf spleen purine nucleoside phosphorylase (PNP) complexes with guanine and 9-deazaguanine.
Nucleosides, nucleotides & nucleic acids26(6-7)841-7(2007)
Stepniak K,Zini? B,Wierzchowski J,Bzowska A

Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase.
Nucleosides, nucleotides & nucleic acids26(6-7)855-9(2007)
Stepniak K,Girstun A,Wielgus-Kutrowska B,Staro? K,Bzowska A

Oligomeric structure of mammalian purine nucleoside phosphorylase in solution determined by analytical ultracentrifugation.
Zeitschrift fur Naturforschung. C, Journal of biosciences60(11-12)927-31(2005 Nov-Dec)
Behlke J,Koellner G,Bzowska A

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PNP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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