CD4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD4
Entrez Gene ID	407098
Full Name	CD4 molecule
General protein information	Preferred Names CD4 molecule Names T-cell surface glycoprotein CD4 CD4 antigen
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024991416.1	XP_024847184.1	T-cell surface glycoprotein CD4 isoform X1
XM_024991417.1	XP_024847185.1	T-cell surface glycoprotein CD4 isoform X1
NM_001103225.1	NP_001096695.1	T-cell surface glycoprotein CD4 precursor
XM_024991418.1	XP_024847186.1	T-cell surface glycoprotein CD4 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cd4	NP_036837.1
Bos taurus (cattle)	CD4	NP_001096695.1
Pan troglodytes (chimpanzee)	CD4	NP_001009043.1
	CD4	NP_001036127.1
Canis lupus familiaris (dog)	CD4	NP_001003252.1
Homo sapiens (human)	CD4	NP_000607.1
Mus musculus (house mouse)	Cd4	NP_038516.1
Gallus gallus (chicken)	CD4	NP_989980.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of CD4 SNPs with fat percentage of Holstein cattle.
Usman T, Yu Y, Zhai L, Liu C, Wang X, Wang Y
Genetics and molecular research : GMR15(3)(2016 Sep)

Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows.
Wang XS, Zhang Y, He YH, Ma PP, Fan LJ, Wang YC, Zhang YI, Sun DX, Zhang SL, Wang CD, Song JZ, Yu Y
Genetics and molecular research : GMR12(4)6228-39(2013 Dec)

Association of bovine CD4 and STAT5b single nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins.
He Y, Chu Q, Ma P, Wang Y, Zhang Q, Sun D, Zhang Y, Yu Y, Zhang Y
The Journal of dairy research78(2)242-9(2011 May)

Subpopulations of bovine WC1(+) gammadelta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo.
Hoek A, Rutten VP, Kool J, Arkesteijn GJ, Bouwstra RJ, Van Rhijn I, Koets AP
Veterinary research40(1)6(2009 Jan-Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that CD4 plays a role in production traits as well as in immune function. The identified SNPs could be used as genetic markers for selection of dairy cows with improved fat percentage. We propose further studies of these SNPs in a larger population as well as further investigations of the function of this gene.
Title: Association of CD4 SNPs with fat percentage of Holstein cattle.

The DNA methylation level of the CD4 gene was strongly influenced by mastitis in Chinese Holstein cattle.
Title: Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows.

These results suggested that the SNPs in CD4 and STAT5b may be potential genetic markers for SCS and milk/protein yields selecting and warrant further functional research.
Title: Association of bovine CD4 and STAT5b single nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins.

These findings revealed that despite the existence of a distinct bovine CD4(+)CD25(high) T cell population, which showed Foxp3 transcription/expression, natural regulatory activity did not reside in this cell population
Title: Subpopulations of bovine WC1(+) gammadelta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo.

The following CD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01507	NM_001103225.1 Latest version!	Bos taurus CD4 molecule (CD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa01507	XM_024991418.1 Latest version!	Bos taurus CD4 molecule (CD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa234199	XM_024991417.1 Latest version!	Bos taurus CD4 molecule (CD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa234199	XM_024991416.1 Latest version!	Bos taurus CD4 molecule (CD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD4 ( NM_001103225.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096695.1 Bos taurus CD4 molecule (CD4), mRNA.

Price & Availability↑

CloneID	OBa01507
Clone ID Related Accession (Same CDS sequence)	NM_001103225.1 , XM_024991418.1
Accession Version	NM_001103225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	T-cell surface glycoprotein CD4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151321.1. On Sep 13, 2007 this sequence version replaced XM_588150.3. ##Evidence-Data-START## Transcript exon combination :: BC151321.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGCGC CGGGCAGAAC GGATGTCTCA AATCAAGAGG CTCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096695.1
CDS	4..1191
Translation	MGPGTSLRHLFLVLQLAMLPAGTQGKTVVLGEAGDKAELPCQASQKKNMVFSWKDSSQSNILGKRGLFFYKGTTELSHRVESKKNLWDQGSFPLIIKNLQVTDSGTYTCEVDKKTLEVELQVFRLTASSDTHVLLGQSLTLTLESPSGSNPSVQWKGPGDNNKRDVKSLSLAQVGLQDSGTWTCTISQSQQTLEIKIPIVVLAFQKAPETVYVKEGEQAEFSFPLTFEYENLSGELTWQLANGDSSSQSWVTFTVKNREVKVNKIHNDPKLLVGEKLPLRLTLPRTLPQHAGSGTLTLDLTKGKLQQKVKLVVMKVTKSPNSLTCEVLGPSPPKLTLNLKMGNQSMKGSNQPKLVTQPEPQAGMWQCLLSDNGKVLLEAKIEAPGRTDVSNQEAP

Target ORF information:

RefSeq Version	NM_001103225.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD4 molecule (CD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103225.1

ORF Insert Sequence:

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGCGC CGGGCAGAAC GGATGTCTCA AATCAAGAGG CTCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD4 ( XM_024991418.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847186.1 Bos taurus CD4 molecule (CD4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa01507
Clone ID Related Accession (Same CDS sequence)	NM_001103225.1 , XM_024991418.1
Accession Version	XM_024991418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	T-cell surface glycoprotein CD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGCGC CGGGCAGAAC GGATGTCTCA AATCAAGAGG CTCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847186.1
CDS	217..1404
Translation	MGPGTSLRHLFLVLQLAMLPAGTQGKTVVLGEAGDKAELPCQASQKKNMVFSWKDSSQSNILGKRGLFFYKGTTELSHRVESKKNLWDQGSFPLIIKNLQVTDSGTYTCEVDKKTLEVELQVFRLTASSDTHVLLGQSLTLTLESPSGSNPSVQWKGPGDNNKRDVKSLSLAQVGLQDSGTWTCTISQSQQTLEIKIPIVVLAFQKAPETVYVKEGEQAEFSFPLTFEYENLSGELTWQLANGDSSSQSWVTFTVKNREVKVNKIHNDPKLLVGEKLPLRLTLPRTLPQHAGSGTLTLDLTKGKLQQKVKLVVMKVTKSPNSLTCEVLGPSPPKLTLNLKMGNQSMKGSNQPKLVTQPEPQAGMWQCLLSDNGKVLLEAKIEAPGRTDVSNQEAP

Target ORF information:

RefSeq Version	XM_024991418.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD4 molecule (CD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991418.1

ORF Insert Sequence:

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGCGC CGGGCAGAAC GGATGTCTCA AATCAAGAGG CTCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD4 ( XM_024991416.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847184.1 Bos taurus CD4 molecule (CD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa234199
Clone ID Related Accession (Same CDS sequence)	XM_024991416.1 , XM_024991417.1
Accession Version	XM_024991416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	T-cell surface glycoprotein CD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGTCT TGCCTTCAGA GTTCACCCAG GCCTGGCCAA AGCTCCTGCC CATGGTGTTG 
GGGGGAATCG CAGGTCTAGT GCTTTTCACT GTTTGCTGCG CCTTCTGTGT TAAATGCTGG 
CACCGCAGGC GCCGGGCAGA ACGGATGTCT CAAATCAAGA GGCTCCTTAG TGAGAAGAAG 
ACCTGCCAGT GCCCCCACCG GCTCCAGAAG ACCTATAGTC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847184.1
CDS	217..1584
Translation	MGPGTSLRHLFLVLQLAMLPAGTQGKTVVLGEAGDKAELPCQASQKKNMVFSWKDSSQSNILGKRGLFFYKGTTELSHRVESKKNLWDQGSFPLIIKNLQVTDSGTYTCEVDKKTLEVELQVFRLTASSDTHVLLGQSLTLTLESPSGSNPSVQWKGPGDNNKRDVKSLSLAQVGLQDSGTWTCTISQSQQTLEIKIPIVVLAFQKAPETVYVKEGEQAEFSFPLTFEYENLSGELTWQLANGDSSSQSWVTFTVKNREVKVNKIHNDPKLLVGEKLPLRLTLPRTLPQHAGSGTLTLDLTKGKLQQKVKLVVMKVTKSPNSLTCEVLGPSPPKLTLNLKMGNQSMKGSNQPKLVTQPEPQAGMWQCLLSDNGKVLLEAKIEVLPSEFTQAWPKLLPMVLGGIAGLVLFTVCCAFCVKCWHRRRRAERMSQIKRLLSEKKTCQCPHRLQKTYSLT

Target ORF information:

RefSeq Version	XM_024991416.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD4 molecule (CD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991416.1

ORF Insert Sequence:

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGTCT TGCCTTCAGA GTTCACCCAG GCCTGGCCAA AGCTCCTGCC CATGGTGTTG 
GGGGGAATCG CAGGTCTAGT GCTTTTCACT GTTTGCTGCG CCTTCTGTGT TAAATGCTGG 
CACCGCAGGC GCCGGGCAGA ACGGATGTCT CAAATCAAGA GGCTCCTTAG TGAGAAGAAG 
ACCTGCCAGT GCCCCCACCG GCTCCAGAAG ACCTATAGTC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD4 ( XM_024991417.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847185.1 Bos taurus CD4 molecule (CD4), transcript variant X2, mRNA.

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CloneID	OBa234199
Clone ID Related Accession (Same CDS sequence)	XM_024991416.1 , XM_024991417.1
Accession Version	XM_024991417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	T-cell surface glycoprotein CD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGTCT TGCCTTCAGA GTTCACCCAG GCCTGGCCAA AGCTCCTGCC CATGGTGTTG 
GGGGGAATCG CAGGTCTAGT GCTTTTCACT GTTTGCTGCG CCTTCTGTGT TAAATGCTGG 
CACCGCAGGC GCCGGGCAGA ACGGATGTCT CAAATCAAGA GGCTCCTTAG TGAGAAGAAG 
ACCTGCCAGT GCCCCCACCG GCTCCAGAAG ACCTATAGTC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847185.1
CDS	203..1570
Translation	MGPGTSLRHLFLVLQLAMLPAGTQGKTVVLGEAGDKAELPCQASQKKNMVFSWKDSSQSNILGKRGLFFYKGTTELSHRVESKKNLWDQGSFPLIIKNLQVTDSGTYTCEVDKKTLEVELQVFRLTASSDTHVLLGQSLTLTLESPSGSNPSVQWKGPGDNNKRDVKSLSLAQVGLQDSGTWTCTISQSQQTLEIKIPIVVLAFQKAPETVYVKEGEQAEFSFPLTFEYENLSGELTWQLANGDSSSQSWVTFTVKNREVKVNKIHNDPKLLVGEKLPLRLTLPRTLPQHAGSGTLTLDLTKGKLQQKVKLVVMKVTKSPNSLTCEVLGPSPPKLTLNLKMGNQSMKGSNQPKLVTQPEPQAGMWQCLLSDNGKVLLEAKIEVLPSEFTQAWPKLLPMVLGGIAGLVLFTVCCAFCVKCWHRRRRAERMSQIKRLLSEKKTCQCPHRLQKTYSLT

Target ORF information:

RefSeq Version	XM_024991417.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD4 molecule (CD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991417.1

ORF Insert Sequence:

ATGGGCCCGG GGACCTCTCT TAGGCACCTG TTCTTGGTGC TGCAACTGGC GATGCTCCCG 
GCTGGCACTC AGGGAAAAAC AGTGGTGCTG GGTGAGGCAG GAGACAAGGC AGAGCTGCCC 
TGCCAGGCTT CCCAGAAGAA GAACATGGTC TTCAGCTGGA AAGATTCTTC CCAGTCCAAT 
ATTCTGGGGA AGCGTGGCCT CTTCTTTTAC AAAGGTACCA CTGAACTGAG CCATCGAGTG 
GAATCCAAAA AAAACCTGTG GGACCAAGGA TCCTTTCCTC TGATCATCAA GAATCTCCAA 
GTAACTGACT CGGGGACTTA TACCTGTGAA GTGGACAAGA AGACGCTCGA GGTGGAACTG 
CAGGTGTTCA GACTGACTGC CAGCTCAGAC ACCCACGTGC TGCTGGGACA GAGCCTGACC 
TTGACTTTGG AGAGTCCCTC TGGGAGCAAC CCTTCCGTGC AATGGAAGGG TCCAGGGGAT 
AATAATAAGA GAGACGTCAA GAGCCTGTCA CTGGCCCAGG TGGGGCTGCA GGACAGTGGT 
ACCTGGACGT GCACTATCTC CCAGAGCCAG CAGACACTGG AGATCAAAAT ACCCATCGTG 
GTGCTGGCCT TCCAGAAGGC CCCCGAAACA GTCTATGTGA AGGAGGGGGA GCAGGCGGAG 
TTTTCCTTCC CACTCACCTT CGAGTATGAA AATCTGAGCG GGGAGCTGAC TTGGCAGCTG 
GCGAACGGGG ATTCTTCCTC CCAATCCTGG GTCACCTTCA CCGTGAAGAA CAGAGAGGTG 
AAGGTGAACA AGATTCACAA CGACCCCAAG CTCCTTGTTG GGGAGAAGCT ACCACTGCGT 
CTCACTCTGC CACGGACTTT GCCTCAGCAC GCGGGTTCTG GAACCCTGAC CCTGGATCTC 
ACCAAGGGGA AGTTGCAGCA GAAAGTGAAA CTCGTGGTGA TGAAAGTGAC TAAGTCCCCA 
AACAGTCTGA CCTGTGAGGT GCTGGGGCCC AGCCCGCCAA AGCTGACCCT GAACTTGAAG 
ATGGGGAATC AGAGTATGAA GGGCTCAAAT CAGCCGAAGT TGGTGACACA GCCGGAGCCC 
CAGGCTGGGA TGTGGCAGTG TCTGCTGAGT GACAATGGCA AAGTCCTGTT GGAAGCCAAG 
ATCGAGGTCT TGCCTTCAGA GTTCACCCAG GCCTGGCCAA AGCTCCTGCC CATGGTGTTG 
GGGGGAATCG CAGGTCTAGT GCTTTTCACT GTTTGCTGCG CCTTCTGTGT TAAATGCTGG 
CACCGCAGGC GCCGGGCAGA ACGGATGTCT CAAATCAAGA GGCTCCTTAG TGAGAAGAAG 
ACCTGCCAGT GCCCCCACCG GCTCCAGAAG ACCTATAGTC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of CD4 SNPs with fat percentage of Holstein cattle.
Genetics and molecular research : GMR15(3)(2016 Sep)
Usman T,Yu Y,Zhai L,Liu C,Wang X,Wang Y

Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows.
Genetics and molecular research : GMR12(4)6228-39(2013 Dec)
Wang XS,Zhang Y,He YH,Ma PP,Fan LJ,Wang YC,Zhang YI,Sun DX,Zhang SL,Wang CD,Song JZ,Yu Y

Association of bovine CD4 and STAT5b single nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins.
The Journal of dairy research78(2)242-9(2011 May)
He Y,Chu Q,Ma P,Wang Y,Zhang Q,Sun D,Zhang Y,Yu Y,Zhang Y

Subpopulations of bovine WC1(+) gammadelta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo.
Veterinary research40(1)6(2009 Jan-Feb)
Hoek A,Rutten VP,Kool J,Arkesteijn GJ,Bouwstra RJ,Van Rhijn I,Koets AP

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CD4 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム