PTI cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTI
Entrez Gene ID	404172
Full Name	pancreatic trypsin inhibitor
Synonyms	BPI,BPTI
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001554.3	NP_001001554.2	pancreatic trypsin inhibitor precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC404103	NP_991355.1
Bos taurus (cattle)	PTI	NP_001001554.2
Bos taurus (cattle)	LOC616039	XP_005215099.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structures of highly simplified BPTIs provide insights into hydration-driven increase of unfolding enthalpy.
Islam MM, Yohda M, Kidokoro SI, Kuroda Y
Scientific reports741205(2017 Mar)

An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis.
Kayode O, Wang R, Pendlebury DF, Cohen I, Henin RD, Hockla A, Soares AS, Papo N, Caulfield TR, Radisky ES
The Journal of biological chemistry291(51)26304-26319(2016 Dec)

Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability?
Moln?r T, V?r?s J, Szeder B, Tak?ts K, Kardos J, Katona G, Gr?f L
The FEBS journal280(22)5750-63(2013 Nov)

X-ray and neutron protein crystallographic analysis of the trypsin-BPTI complex.
Kawamura K, Yamada T, Kurihara K, Tamada T, Kuroki R, Tanaka I, Takahashi H, Niimura N
Acta crystallographica. Section D, Biological crystallography67(Pt 2)140-8(2011 Feb)

Thermodynamic and structural analysis of highly stabilized BPTIs by single and double mutations.
Islam MM, Sohya S, Noguchi K, Kidokoro S, Yohda M, Kuroda Y
Proteins77(4)962-70(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the importance of substrate dynamics in the cleavage of Kunitz-bovine pancreatic trypsin inhibitor protease inhibitors by mesotrypsin, finding that the varied conformational dynamics of structurally similar substrates can profoundly impact the rate of catalysis.
Title: An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis.

analyzed the mechanisms behind a remarkable 13 degrees stabilization produced by a single A14G and a double A14GA38V mutation
Title: Thermodynamic and structural analysis of highly stabilized BPTIs by single and double mutations.

variation of the radius of gyration and the modification of the shape of BPTI between ambient temperature and 368 K
Title: Temperature dependence on structure and dynamics of Bovine Pancreatic Trypsin Inhibitor (BPTI): a neutron scattering study.

insights into the mechanism by which inhibitors like BPTI normally resist hydrolysis when bound to their target proteases.
Title: Structure of a serine protease poised to resynthesize a peptide bond.

high-resolution crystal structures of an extensively simplified variant of bovine pancreatic trypsin inhibitor containing 20 alanines.
Title: Crystal structure of an extensively simplified variant of bovine pancreatic trypsin inhibitor in which over one-third of the residues are alanines.

The following PTI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41247	NM_001001554.3 Latest version!	Bos taurus pancreatic trypsin inhibitor (PTI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTI ( NM_001001554.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001001554.2 Bos taurus pancreatic trypsin inhibitor (PTI), mRNA.

Price & Availability↑

CloneID	OBa41247
Clone ID Related Accession (Same CDS sequence)	NM_001001554.3
Accession Version	NM_001001554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Bos taurus(cattle)
Product	pancreatic trypsin inhibitor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149368.1. On Aug 31, 2012 this sequence version replaced NM_001001554.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC149368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAAGATGA GCCGGCTCTG CCTCTCAGTA GCCCTTCTGG TCCTCCTGGG CACCCTGGCG 
GCCAGCACTC CAGGGTGTGA CACCAGCAAC CAGGCCAAAG CCCAGCGGCC TGACTTCTGC 
CTAGAGCCTC CATATACGGG TCCCTGCAAG GCCAGAATGA TCAGATACTT CTACAACGCC 
AAGGCTGGGC TCTGCCAGCC CTTTGTATAT GGCGGCTGCA GAGCTAAAAG AAACAATTTC 
AAGAGCTCAG AGGACTGCAT GAGGACCTGT GGTGGTGCTA TTGGGCCCTG GGAGAACCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001554.2
CDS	22..324
Translation	MKMSRLCLSVALLVLLGTLAASTPGCDTSNQAKAQRPDFCLEPPYTGPCKARMIRYFYNAKAGLCQPFVYGGCRAKRNNFKSSEDCMRTCGGAIGPWENL

Target ORF information:

RefSeq Version	NM_001001554.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pancreatic trypsin inhibitor (PTI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001554.3

ORF Insert Sequence:

ATGAAGATGA GCCGGCTCTG CCTCTCAGTA GCCCTTCTGG TCCTCCTGGG CACCCTGGCG 
GCCAGCACTC CAGGGTGTGA CACCAGCAAC CAGGCCAAAG CCCAGCGGCC TGACTTCTGC 
CTAGAGCCTC CATATACGGG TCCCTGCAAG GCCAGAATGA TCAGATACTT CTACAACGCC 
AAGGCTGGGC TCTGCCAGCC CTTTGTATAT GGCGGCTGCA GAGCTAAAAG AAACAATTTC 
AAGAGCTCAG AGGACTGCAT GAGGACCTGT GGTGGTGCTA TTGGGCCCTG GGAGAACCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structures of highly simplified BPTIs provide insights into hydration-driven increase of unfolding enthalpy.
Scientific reports741205(2017 Mar)
Islam MM,Yohda M,Kidokoro SI,Kuroda Y

An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis.
The Journal of biological chemistry291(51)26304-26319(2016 Dec)
Kayode O,Wang R,Pendlebury DF,Cohen I,Henin RD,Hockla A,Soares AS,Papo N,Caulfield TR,Radisky ES

Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability?
The FEBS journal280(22)5750-63(2013 Nov)
Moln?r T,V?r?s J,Szeder B,Tak?ts K,Kardos J,Katona G,Gr?f L

X-ray and neutron protein crystallographic analysis of the trypsin-BPTI complex.
Acta crystallographica. Section D, Biological crystallography67(Pt 2)140-8(2011 Feb)
Kawamura K,Yamada T,Kurihara K,Tamada T,Kuroki R,Tanaka I,Takahashi H,Niimura N

Thermodynamic and structural analysis of highly stabilized BPTIs by single and double mutations.
Proteins77(4)962-70(2009 Dec)
Islam MM,Sohya S,Noguchi K,Kidokoro S,Yohda M,Kuroda Y

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PTI cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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