CYB561 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYB561
Entrez Gene ID	317663
Full Name	cytochrome b561
General protein information	Preferred Names cytochrome b561 Names cytochrome b561 cytochrome b-561
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010816315.2	XP_010814617.1	cytochrome b561 isoform X1
XM_005220821.4	XP_005220878.1	cytochrome b561 isoform X1
XM_005220821.3	XP_005220878.1	cytochrome b561 isoform X1
XM_005220819.3	XP_005220876.1	cytochrome b561 isoform X1
XM_005220819.2	XP_005220876.1	cytochrome b561 isoform X1
XM_010816315.3	XP_010814617.1	cytochrome b561 isoform X1
NM_174837.2	NP_777262.1	cytochrome b561

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	CELE_F55H2.5	NP_499095.1
Canis lupus familiaris (dog)	CYB561	XP_005624297.1
Danio rerio (zebrafish)	cyb561	NP_001122216.2
Arabidopsis thaliana (thale cress)	ACYB-2	NP_567723.1
Homo sapiens (human)	CYB561	NP_001017916.1
Pan troglodytes (chimpanzee)	CYB561	XP_511596.3
Bos taurus (cattle)	CYB561	NP_777262.1
Oryza sativa (rice)	Os04g0533500	NP_001053410.1
Xenopus tropicalis (tropical clawed frog)	cyb561	NP_001005633.1
Mus musculus (house mouse)	Cyb561	NP_031831.2
Rattus norvegicus (Norway rat)	Cyb561	NP_001100526.1
Gallus gallus (chicken)	CYB561	XP_418073.3
Oryza sativa (rice)	Os10g0504200	NP_001065002.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional and structural roles of residues in the third extramembrane segment of adrenal cytochrome b561.
Liu W, da Silva GF, Wu G, Palmer G, Tsai AL, Kulmacz RJ
Biochemistry50(15)3149-60(2011 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

His92 and His110 selectively affect different heme centers of adrenal cytochrome b(561).
Liu W, Rogge CE, da Silva GF, Shinkarev VP, Tsai AL, Kamensky Y, Palmer G, Kulmacz RJ
Biochimica et biophysica acta1777(9)1218-28(2008 Sep)

Axial ligation and stoichiometry of heme centers in adrenal cytochrome b561.
Kamensky Y, Liu W, Tsai AL, Kulmacz RJ, Palmer G
Biochemistry46(29)8647-58(2007 Jul)

Selective perturbation of the intravesicular heme center of cytochrome b561 by cysteinyl modification with 4,4'-dithiodipyridine.
Takeuchi F, Hori H, Tsubaki M
Journal of biochemistry138(6)751-62(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the functional and structural roles of the EM3 residues and several adjacent residues (residues 70-85) in the bovine cytochrome
Title: Functional and structural roles of residues in the third extramembrane segment of adrenal cytochrome b561.

These results indicate that His92 can be considered part of the b(H) heme center, and His110 part of the b(L) heme center, in adrenal cyt b(561).
Title: His92 and His110 selectively affect different heme centers of adrenal cytochrome b(561).

a detailed characterization of cyt b561 using electron paramagnetic resonance (EPR) and optical spectroscopy on the wild-type and mutant forms of the cytochrome expressed in insect cells
Title: Axial ligation and stoichiometry of heme centers in adrenal cytochrome b561.

importance of the two well-conserved Cys residues near the intravesicular heme center
Title: Selective perturbation of the intravesicular heme center of cytochrome b561 by cysteinyl modification with 4,4'-dithiodipyridine.

Raman data indicate that the two heme b centers of CYB561 adopt a six-coordinated low-spin state, in both reduced and oxidized forms.
Title: Properties of two distinct heme centers of cytochrome b561 from bovine chromaffin vesicles studied by EPR, resonance Raman, and ascorbate reduction assay.

The following CYB561 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYB561 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41372	NM_174837.2 Latest version!	Bos taurus cytochrome b561 (CYB561), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa30098	XM_005220821.4 Latest version!	Bos taurus cytochrome b561 (CYB561), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa30098	XM_010816315.3 Latest version!	Bos taurus cytochrome b561 (CYB561), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa30098	XM_005220819.3 Latest version!	Bos taurus cytochrome b561 (CYB561), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa30098	XM_005220821.3	Bos taurus cytochrome b-561 (CYB561), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa30098	XM_005220819.2	Bos taurus cytochrome b-561 (CYB561), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa30098	XM_010816315.2	Bos taurus cytochrome b-561 (CYB561), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYB561 ( NM_174837.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777262.1 Bos taurus cytochrome b561 (CYB561), mRNA.

Price & Availability↑

CloneID	OBa41372
Clone ID Related Accession (Same CDS sequence)	NM_174837.2
Accession Version	NM_174837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	cytochrome b561
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X12783.1. On Jun 3, 2003 this sequence version replaced NM_174837.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X12783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTGGAGGA CCTTGACTTG CACCCTCACT GCAGGTGGAA GTGCAGCCAG TAGCCGCCTC 
AGCATGGAGG GCCCTGCCAG CCCAGCACGT GCCCCCGGGG CGCTGCCCTA CTACGTGGCC 
TTCTCCCAGC TGCTGGGCCT GATCGTGGTG GCCATGACTG GCGCCTGGCT TGGTATGTAC 
CGAGGCGGCA TTGCCTGGGA GAGTGCCCTG CAGTTCAACG TGCACCCCCT CTGCATGATC 
ATAGGCCTGG TCTTCCTGCA GGGAGATGCC CTGCTGGTTT ACCGCGTCTT CAGGAATGAG 
GCCAAACGCA CCACCAAAGT CCTGCACGGG CTGCTGCACG TCTTCGCCTT CGTCATCGCC 
TTGGTGGGCC TGGTGGCGGT GTTCGAGCAC CACAGGAAGA AGGGCTACGC CGACCTGTAC 
AGCCTGCACA GCTGGTGCGG CATCCTGGTC TTCGCGCTCT TCTTTGCGCA GTGGCTCGTG 
GGCTTTAGCT TCTTCCTGTT CCCGGGCGCC TCGTTTTCTC TGCGGAGCCG CTACCGCCCG 
CAGCACGTCT TCTTCGGCGC CGCCATCTTC CTGCTCTCGG TGGCCACCGC CCTGCTGGGC 
CTGAAGGAGG CGCTGCTGTT CGAGCTCGGG ACCAAGTACA GCATGTTCGA GCCCGAGGGC 
GTCCTGGCCA ACGTGCTGGG CCTGCTGCTG GCCACCTTTG CCACGGTCAT ACTCTACATC 
CTGACCCGCG CCGACTGGAA GCGGCCCCTC CAGGCGGAAG AGCAAGCGCT CTCCATGGAC 
TTCAAGACGC TGACGGAGGG CGACAGCCCC AGCTCCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777262.1
CDS	91..912
Translation	MWRTLTCTLTAGGSAASSRLSMEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	NM_174837.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b561 (CYB561), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174837.2

ORF Insert Sequence:

ATGTGGAGGA CCTTGACTTG CACCCTCACT GCAGGTGGAA GTGCAGCCAG TAGCCGCCTC 
AGCATGGAGG GCCCTGCCAG CCCAGCACGT GCCCCCGGGG CGCTGCCCTA CTACGTGGCC 
TTCTCCCAGC TGCTGGGCCT GATCGTGGTG GCCATGACTG GCGCCTGGCT TGGTATGTAC 
CGAGGCGGCA TTGCCTGGGA GAGTGCCCTG CAGTTCAACG TGCACCCCCT CTGCATGATC 
ATAGGCCTGG TCTTCCTGCA GGGAGATGCC CTGCTGGTTT ACCGCGTCTT CAGGAATGAG 
GCCAAACGCA CCACCAAAGT CCTGCACGGG CTGCTGCACG TCTTCGCCTT CGTCATCGCC 
TTGGTGGGCC TGGTGGCGGT GTTCGAGCAC CACAGGAAGA AGGGCTACGC CGACCTGTAC 
AGCCTGCACA GCTGGTGCGG CATCCTGGTC TTCGCGCTCT TCTTTGCGCA GTGGCTCGTG 
GGCTTTAGCT TCTTCCTGTT CCCGGGCGCC TCGTTTTCTC TGCGGAGCCG CTACCGCCCG 
CAGCACGTCT TCTTCGGCGC CGCCATCTTC CTGCTCTCGG TGGCCACCGC CCTGCTGGGC 
CTGAAGGAGG CGCTGCTGTT CGAGCTCGGG ACCAAGTACA GCATGTTCGA GCCCGAGGGC 
GTCCTGGCCA ACGTGCTGGG CCTGCTGCTG GCCACCTTTG CCACGGTCAT ACTCTACATC 
CTGACCCGCG CCGACTGGAA GCGGCCCCTC CAGGCGGAAG AGCAAGCGCT CTCCATGGAC 
TTCAAGACGC TGACGGAGGG CGACAGCCCC AGCTCCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB561 ( XM_010816315.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814617.1 Bos taurus cytochrome b-561 (CYB561), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30098
Clone ID Related Accession (Same CDS sequence)	XM_010816315.2 , XM_005220821.4 , XM_005220821.3 , XM_005220819.3 , XM_005220819.2 , XM_010816315.3
Accession Version	XM_010816315.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytochrome b561 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814617.1
CDS	297..1055
Translation	MEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	XM_010816315.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b-561 (CYB561), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816315.2

ORF Insert Sequence:

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB561 ( XM_005220821.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220878.1 Bos taurus cytochrome b561 (CYB561), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30098
Clone ID Related Accession (Same CDS sequence)	XM_010816315.2 , XM_005220821.4 , XM_005220821.3 , XM_005220819.3 , XM_005220819.2 , XM_010816315.3
Accession Version	XM_005220821.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytochrome b561 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220821.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220878.1
CDS	91..849
Translation	MEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	XM_005220821.4
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b561 (CYB561), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220821.4

ORF Insert Sequence:

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB561 ( XM_005220821.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220878.1 Bos taurus cytochrome b-561 (CYB561), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30098
Clone ID Related Accession (Same CDS sequence)	XM_010816315.2 , XM_005220821.4 , XM_005220821.3 , XM_005220819.3 , XM_005220819.2 , XM_010816315.3
Accession Version	XM_005220821.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytochrome b561 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220878.1
CDS	91..849
Translation	MEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	XM_005220821.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b-561 (CYB561), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220821.3

ORF Insert Sequence:

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB561 ( XM_005220819.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220876.1 Bos taurus cytochrome b561 (CYB561), transcript variant X1, mRNA.

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CloneID	OBa30098
Clone ID Related Accession (Same CDS sequence)	XM_010816315.2 , XM_005220821.4 , XM_005220821.3 , XM_005220819.3 , XM_005220819.2 , XM_010816315.3
Accession Version	XM_005220819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytochrome b561 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220876.1
CDS	169..927
Translation	MEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	XM_005220819.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b561 (CYB561), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220819.3

ORF Insert Sequence:

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB561 ( XM_005220819.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220876.1 Bos taurus cytochrome b-561 (CYB561), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30098
Clone ID Related Accession (Same CDS sequence)	XM_010816315.2 , XM_005220821.4 , XM_005220821.3 , XM_005220819.3 , XM_005220819.2 , XM_010816315.3
Accession Version	XM_005220819.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytochrome b561 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220876.1
CDS	174..932
Translation	MEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	XM_005220819.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b-561 (CYB561), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220819.2

ORF Insert Sequence:

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYB561 ( XM_010816315.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814617.1 Bos taurus cytochrome b561 (CYB561), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30098
Clone ID Related Accession (Same CDS sequence)	XM_010816315.2 , XM_005220821.4 , XM_005220821.3 , XM_005220819.3 , XM_005220819.2 , XM_010816315.3
Accession Version	XM_010816315.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytochrome b561 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814617.1
CDS	291..1049
Translation	MEGPASPARAPGALPYYVAFSQLLGLIVVAMTGAWLGMYRGGIAWESALQFNVHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFELGTKYSMFEPEGVLANVLGLLLATFATVILYILTRADWKRPLQAEEQALSMDFKTLTEGDSPSSQ

Target ORF information:

RefSeq Version	XM_010816315.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cytochrome b561 (CYB561), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816315.3

ORF Insert Sequence:

ATGGAGGGCC CTGCCAGCCC AGCACGTGCC CCCGGGGCGC TGCCCTACTA CGTGGCCTTC 
TCCCAGCTGC TGGGCCTGAT CGTGGTGGCC ATGACTGGCG CCTGGCTTGG TATGTACCGA 
GGCGGCATTG CCTGGGAGAG TGCCCTGCAG TTCAACGTGC ACCCCCTCTG CATGATCATA 
GGCCTGGTCT TCCTGCAGGG AGATGCCCTG CTGGTTTACC GCGTCTTCAG GAATGAGGCC 
AAACGCACCA CCAAAGTCCT GCACGGGCTG CTGCACGTCT TCGCCTTCGT CATCGCCTTG 
GTGGGCCTGG TGGCGGTGTT CGAGCACCAC AGGAAGAAGG GCTACGCCGA CCTGTACAGC 
CTGCACAGCT GGTGCGGCAT CCTGGTCTTC GCGCTCTTCT TTGCGCAGTG GCTCGTGGGC 
TTTAGCTTCT TCCTGTTCCC GGGCGCCTCG TTTTCTCTGC GGAGCCGCTA CCGCCCGCAG 
CACGTCTTCT TCGGCGCCGC CATCTTCCTG CTCTCGGTGG CCACCGCCCT GCTGGGCCTG 
AAGGAGGCGC TGCTGTTCGA GCTCGGGACC AAGTACAGCA TGTTCGAGCC CGAGGGCGTC 
CTGGCCAACG TGCTGGGCCT GCTGCTGGCC ACCTTTGCCA CGGTCATACT CTACATCCTG 
ACCCGCGCCG ACTGGAAGCG GCCCCTCCAG GCGGAAGAGC AAGCGCTCTC CATGGACTTC 
AAGACGCTGA CGGAGGGCGA CAGCCCCAGC TCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional and structural roles of residues in the third extramembrane segment of adrenal cytochrome b561.
Biochemistry50(15)3149-60(2011 Apr)
Liu W,da Silva GF,Wu G,Palmer G,Tsai AL,Kulmacz RJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

His92 and His110 selectively affect different heme centers of adrenal cytochrome b(561).
Biochimica et biophysica acta1777(9)1218-28(2008 Sep)
Liu W,Rogge CE,da Silva GF,Shinkarev VP,Tsai AL,Kamensky Y,Palmer G,Kulmacz RJ

Axial ligation and stoichiometry of heme centers in adrenal cytochrome b561.
Biochemistry46(29)8647-58(2007 Jul)
Kamensky Y,Liu W,Tsai AL,Kulmacz RJ,Palmer G

Selective perturbation of the intravesicular heme center of cytochrome b561 by cysteinyl modification with 4,4'-dithiodipyridine.
Journal of biochemistry138(6)751-62(2005 Dec)
Takeuchi F,Hori H,Tsubaki M

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CYB561 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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