SSPO cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SSPO
Entrez Gene ID	282659
Full Name	SCO-spondin
General protein information	Preferred Names SCO-spondin Names SCO-spondin SCO-spondin homolog (Bos taurus)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174706.2	NP_777131.1	SCO-spondin precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SSPO	NP_777131.1
Rattus norvegicus (Norway rat)	Sspo	NP_001007017.1
Homo sapiens (human)	SSPO	NP_940857.2
Gallus gallus (chicken)	SSPO	NP_001006351.2
Canis lupus familiaris (dog)	SSPO	NP_001075179.1
Danio rerio (zebrafish)	scospondin	XP_001920334.4
Xenopus tropicalis (tropical clawed frog)	LOC100497129	XP_002942623.2
Pan troglodytes (chimpanzee)	SSPO	XP_003951224.1
	LOC712419	XP_002808415.1
Mus musculus (house mouse)	Sspo	NP_775604.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The lengthening of a giant protein: when, how, and why?
Meiniel O, Meiniel R, Lallou? F, Didier R, Jauberteau MO, Meiniel A, Petit D
Journal of molecular evolution66(1)1-10(2008 Jan)

The complex multidomain organization of SCO-spondin protein is highly conserved in mammals.
Meiniel O, Meiniel A
Brain research reviews53(2)321-7(2007 Feb)

Transcription of SCO-spondin in the subcommissural organ: evidence for down-regulation mediated by serotonin.
Richter HG, Tom? MM, Yulis CR, V?o KJ, Jim?nez AJ, P?rez-F?gares JM, Rodr?guez EM
Brain research. Molecular brain research129(1-2)151-62(2004 Oct)

Bovine floor plate explants secrete SCO-spondin.
Gui?az? MF, Richter HG, Rodr?guez EM
Cell and tissue research308(2)177-91(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that 5HT down-regulates SCO-spondin biosynthesis but apparently not its release, and suggest that 5HT may exert the effect on the SCO via the cerebrospinal fluid.
Title: Transcription of SCO-spondin in the subcommissural organ: evidence for down-regulation mediated by serotonin.

expression of SCO-spondin is not restricted to the subcommissural organ but it also occurs in the floor plate, a key structure participating in neuronal differentiation and patterning of the neural tube
Title: Bovine floor plate explants secrete SCO-spondin.

role in CNS development
Title: Subcommissural organ/Reissner's fiber complex: characterization of SCO-spondin, a glycoprotein with potent activity on neurite outgrowth.

The following SSPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41491	NM_174706.2 Latest version!	Bos taurus SCO-spondin (SSPO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSPO ( NM_174706.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777131.1 Bos taurus SCO-spondin (SSPO), mRNA.

Price & Availability↑

CloneID	OBa41491
Clone ID Related Accession (Same CDS sequence)	NM_174706.2
Accession Version	NM_174706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Bos taurus(cattle)
Product	SCO-spondin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ416457.1. On or before Mar 29, 2005 this sequence version replaced XM_606520.1, XM_617586.1, NM_174706.1. ##Evidence-Data-START## Transcript exon combination :: AJ416457.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTCC CTGCCCTCCT CTTCGGGGCG GCATGGGCAC TGGCTAATGG GCGGTGGTGT 
GAGCAGACAG AGACCGTCCT GGTGGAGGAG GAAGTCACCC CCCATCAGGA GGACCTGGTG 
CCCTGTGCCA GCCTCCAGCA TTACCAGCGC CGGGGCTGGC GGCTGGACCT GACCTGGAGT 
GGCCGTGCGG GGCTCTGTGC CATCTACAAA CCGCCAGAGA CCCGGCCTGC TGCCTGGAAC 
CGGACAGTGA GGGCCTGCTG CCCAGGCTGG GGGGGCACCC ACTGCACCCT AGCCCTTGCA 
GAGGCCAGCC CTGAGGGCCA CTGCTTTGCC ACCTGGCTGT GCAACCTGGG GGCAGGCTCA 
GTGAATGCGT CTGCAGGAAG TCTGGAGGAG TGCTGTGCCC AACCCTGGGG ACACAGCTGG 
CGGGATGGCC GTTCCCAGAC CTGCCACAGC TGCTCCAACC ACAGTCGACT GGGCAGCACA 
CCCTCCCCAG CCATCCTGCA GCCCCTGGCG GGGGCTGTGG CCCAGCTCTG GAGCCAGCGC 
CAGCGTCCCT CGGCCACCTG CGCCACCTGG TCCGGCTTCC ACTATCGCAC CTTCGATGGA 
CGCCACTTCC ACTTCCTGGG CCGCTGCACC TACCTGCTGG CGGGTGCGGC TGATGCCACC 
TGGGCCGTCC ACCTCCAGCC CATGGGGCAT TGTCCCCAGC CTGGGCACTG TCAGCTGGCC 
CGGGTAATGA TGGGACCAGA GGAGGTGCTG ATCCGGGGTG AAAACGTCAC TGTGAACGGG 
CGGCTGGTAC CCGAGGGGGC GTCTCAGCTG CTCCCTGGGC TCAGCCTGCA GTGGCAAGGG 
GACTGGCTGG TGCTGTCGGG AGGCCTGGGG GTCGTTGTGC GGCTGGACCG GTCCAGTTCC 
GTCTCCATTT CTGTGGACCA GGAGCTCCAG GGACAGACTC AAGGCCTCTG TGGGGTCTAC 
AACGGCCAGC CTGAGGATGA CTTCCTAGAG CCTGGCAGGG GACTGGCTGC GTTAGCTGCC 
ACCTTTGGGA ATTCCTGGAG ACTCCCAGAC TCAGAGCTTG GGTGTCTGGA TGCGGTGGAG 
GCAGCTCAAG GCTGTGAGGA CCCCCTGAGG GGCACAGAGA CGGGCACGGA GGCTGGCCAG 
CTTCGGGCTG AAGCCCAGGA TGTGTGCCAC CAGCTGCTGG AAGGCCCATT CAGAGAATGT 
CACACCCAGG TGCCCCCTGC AGAGTACCAC GAGGCCTGCC TCTTCGCCTA CTGTGCTGGG 
GCCCCAGCGG GCAGTGGGCG GGCAGAGCGG CTGGAGGCTG TGTGCGCCAC CCTGGCCAGC 
TACGCCCAGG ACTGTGCGGC TAGGCGCATC GCGGTGCGCT GGAGGAAGCC GGGCTTCTGC 
GAGCGCCTGT GTCCGGGGGG CCAGCTGTAC TCCGACTGCG CCTCGGCCTG CCCGCCCAGC 
TGCTCGGCAG TGGGGGAGGG AAGCGAATGG AGCTGCGGGG AAGAATGCGT GAGCGGCTGC 
GAGTGCCCGC CGGGTCTCTT CTGGGACGGC GCCCTGTGCG TGCCCGCCGC CCGCTGCCCC 
TGCTACCGCC GACGCCGGCG CTACGAGCCC GGGGACACCG TGCGCCAGCT CTGCAACCCG 
TGCGAGTGCA GGGACGGCCG CTGGCTCTGC GCGCAGGCGC CGTGCGCCGC CGAGTGCGCG 
GTAGGCGGGG ACGGGCATTA CGTCACCTTC GATGGGCGGA GCTTCTCCTT CCGCGGCCGC 
GCCGGTTGCC GCTTCATCCT GGTGCAGGAC TTTGCCAAGC GGCAGCTGCT GATTGTACTG 
GAACACGGGG ACTGTGACGC TGGGAGCTGC CTCCATGCCA TCTCTGTCTC CCTGGGGGAC 
ACCCTCGTCC AGCTCAGGGA CTCAGGAGTT GTGCTGGTCG ACGGTCAGGA TGTGGCCTTG 
CCCTGGAGTG CAGCTGGGGG CCTCAGTGTC TCCCGGGCCT CTTCCTCTTT CCTGCTGCTG 
CGCTGGCCTG GTGCCCGCAT CCTCTGGGGA GTGTCTGACC CTGCAGCCTA CATCACCCTG 
GACCCCCACC ACGCCCACCA GGTGCAGGGT CTGTGTGGCA CCTTCACCCG GAATCAGCAG 
GATGACTTCC TGACCCCTGC TGGCGATGTG GAGACTAGTA TCACCGCCTT TGCTAGCAAG 
TTCCAGGTGG CAGGCGGGGG AACGTGCTCC TTGGAGGCCT GCACTCCGCT TTCCCCCTGC 
AGCACCCACA CTGAGCGCCA GGTTTTCGCG GAGGTAGCCT GTGCCATCCT GCATGGCCCA 
ACCTTCCAGG AGTGCCACGG GCTGGTGGAC AGGGAGGCGT TCCACCTGCG CTGCTTGGCA 
GCTGTGTGTG GCTGCACCCC TGGCCGGGAC TGCCTGTGTC CCGTGCTTGC GGCCTATGCT 
CGGCGCTGTG CCCAGGAGGG TGCCCTGCCT TCCTGGAGAA ACCAGACCTT CTGCCCTGTC 
CTGTGTCCTG GTGGCCAGGA GTACCAGGAG TGCGCCCCAG CATGCGATCG CAACTGCGGG 
GAGCCTGAAG ACTGTGGGGA GCTGGACAAC TGTGTGGCCG GCTGTAATTG CCCCCTAGGG 
CTGCTGTGGG ACCCCGAGGG CCAGTGTGTG CCCCCCAACT TGTGCCCCTG CCAGCTTGGG 
GCCCATCGCT ATGCCCCTGG CAGTGCTACT ATGAAGGACT GCAACCACTG TGTCTGCCAG 
GAGAGGGGCC TCTGGAACTG CACTGCTCAC CACTGTGCTC CGCCCCGGAC CTTCTGCCCC 
CGTGAGCTTG TCTATGTCCC TGGAGCCTGC CTCCTCACCT GTGACAGCCT TGATGCCGAC 
CGCACCTGCC CCCCAGGCAG CCCGGGGGGC TGTGTCTGCC CCCCAGGCAC CGTGCTGCTG 
GAGGAACGCT GCGTGCCTCC TGAGCTCTGT CCCTGCCGTC ATGGCGGCCA GTGGTACCTG 
CCCAATGCTG CAATCCAGGA GGACTGCAAC CTGTGCGTGT GCCAGGGTCA GCAGTGGCAC 
TGCACGGGCC AGCGGTGCGA TGGACGGTGC CGGGCGTCAG GCGCCCCTCA CTATGTGACG 
TTCGACGGGC TGGCCCTCAC CTTCCCCGGG GCCTGCGAGT ACCTGCTGGT GCGAGAGGCC 
AGTGGCCAGT TCATGGTCTC CGCCCAAAAC CTGCCGTGCG GAGCCAGTGG CCTCACCTGC 
ACCAAGGCGC TGACTGTGCG GCTGCAGGGC ACCGTTGTGC ACATGCTCAG AGGCAGGGCA 
GTGATGGTGA ATGGGGTGAG TGTGACGCCC CCCAAGGTCT ACTCAGGCCC CGGGCTGAGC 
CTGCACACCG CCGGCCTCTT CCTGCTTCTC AGCACCCGCC TGGGCCTCAC CTTGCTCTGG 
GATGGAGGCA CCCGGGTCCC GGTGCAGCTG TCCCCCCAGC TCCGCGGCAG GGTGGCTGGC 
CTGTGTGGAG ACTTCGATGG AGATGCCAGT AACGACCTTC GGAGCCGCCA GGGGGTCCTG 
GAGCCCACAG CTGAACTGGC CGCCCACTCC TGGCGCCTCG GTCCTCTCTG CCCAGAGCCA 
GGGGACCTGC CACATCCCTG TGCAGTGAAT GCCCACCGGG CTGGCTGGGC CCGCGCCCGC 
TGCGGGGTGG TGCTGCAGCC GCTCTTTGCC CGGTGCCACG TGGAGGTGCC CCCGCAGCAG 
CACTACGAGC AGTGTGTGTA TGATGCCTGC GGCTGCGATT CGGGGGGCGA CTGTGAGTGT 
CTCTGCTCGG CCATTGCCAC CTATGCAGAT GAGTGTGCCC GGCACGGGAT CCACGTGCGC 
TGGCGCAGCC AGGAGCTCTG CCCTCTGCAG TGTGAGAGGG GCCAGGTGTA TGAGGCCTGT 
GGCCCCACGT GCCCCGCCAC CTGCCACGAC CACCGTCCTG AGCCTGGGTG GCCCTGCCGG 
GCCGTCGCCT GTGTGGAGGG CTGCTTCTGC CCTGAGGGGA CTCTGTTGCA TGGAGGTGTC 
TGCTTGGAGC CGGCTGCCTG CCCCTGCGAG TGGGGGGGCA GCTTCTTCCC ACCGGGCACG 
GTGCTGCAGA AGGACTGTGG CAACAACTGC ACGTGTCGGG AAAGTCAGTG GCTTTGTGGG 
GACGATGGCG GCCGCTGTGT GGAGCCCGGG CCTGGTTGTG CGGAGGGAGA GACCCCATGC 
CGCGAGAGCG GGCACTGTGT GCCCCACGGG TGGCTCTGTG ACAACCAGGA TGACTGTGGC 
GATGGCTCAG ACGAGGAGGG CTGTGCCACC CGGGTCTGTG GCGAGGGGCA GGTGTCTTGC 
TGCTCCGGCC GCTGCCTGCC CCTGGTCCTG CTCTGTGATG GGCAGGACGA CTGTGGAGAC 
GGGATGGATG AGCAGGGCTG CCCGTGCCCC CAGGACTCGC TGACCTGTGC CGACGGGCAC 
TGCCTGCCCC CGGCCCGGCT CTGTGACGGG CACCCCGACT GTCCGGATGG TGCTGACGAG 
GAGTCTTGCC TGGGGCAAGT GGACTGCGCC CCTGGGGAGG TGTCCTGCGT CGATGGTACC 
TGCCTGGGGG CCATCCAGCT GTGTGATGGA GTCTGGGACT GTCTGGATGG TGGCGATGAG 
GGGCCTGGGC ACTGCCCCCT CCCCTCTCTG CCCACTCCTC CTGCTGGTAC CTTGCCTGGC 
CCCTCTGCTG TCTCCTGGAA ACTGCACCTA CCCCCCTGGC CAGTGTCGGC CCTGCGCTTG 
CCCTGCGGCC CCTTGGACTT CGCGTGCGGC AGCGGCGAGT GCGCGCCCCG GGGCTGGCGC 
TGCGACGGCG AGGAGGACTG CGCCGACGGC AGCGACGAGA GCGGCTGCGA CCGGCCCTGC 
GCGCCGCACC ACGCGCCCTG CGCGCGCGGC TCGCACTGCG TGGCCGCGGA GCAGCTGTGC 
GACGGCGTCC CGCATTGCCC GGACGGCTCA GACGAGGACC CGGGCGCCTG CGAGAGGCTG 
CAGGCCCCAG GAGGCCCCAA CCGGACAGGG CTTCCCTGCC CAGAATACTC CTGCCCGGAT 
GGCCTCTGCA TCGGTTTCCA GCAGGTGTGC GATGGGCAGC CCGACTGTGA GCTGGCGGGG 
ACAGCAGGGC CCTCCCCAGA GGAGCAGGGC TGTGGGGCCT GGGGGCCCTG GAGCCCCTGG 
GAGCTGTGCA GCCGGACATG TGGGCCCGGG GTGCAGGGCT GGAGTCGCCG CTGCTCGCCC 
CCGAGCCTCC CGGTGCTGTG GCACTGCCCC GGCCCAGAGC GCCAGACTCG GGCCTGCTTC 
GCGGCCGCCT GCCCAGAGGA CGGTGTATGG ACCTCCTGGT CGCGCTGGTC TCCATGCTCT 
GAGCCATGCG GGGGCGTCAC GGCTCGCCAC CGGGAGTGCC ATCCGCCCCA GAATGGTGGC 
CGGACCTGTG CCACGCTGCC TGGGGGCCCT CCCAGCACCC GGGAGACCCG GCCCTGCCCT 
CAGGATGGCT GCCCCAACGT CACCTGCTCT GGGGAGCTGG TGTTCCATGC CTGTGTTCCT 
TGCCCTTTGA CCTGTGATGA CATCTCTGGT CAGGCCACGT GCCCCCCCGA CCGGCCCTGT 
GGTGGCCCAG GCTGCTGGTG CCCTGCGGGG CAGGTGCTGG GTGCCCAGGG GCGGTGCGTG 
TGGCCACGGC AATGCCCCTG CCTGGTGGAC GGCAGCCGCT ACTGGCCCGG GCAGCGTGTC 
AAGACTGATT GCCAGCTCTG CGTCTGCCAG GACGGGCGGC CCCGGCGCTG CCAGCCCAGC 
CTAGACTGTG CAGTGAACTG CGGCTGGTCA GCGTGGTCCC CCTGGGCTGA GTGCCTGGGC 
CCCTGTGGCA GCCGGAGCGT CCAGTGGTCC TTCCGGAGCC CCAACAACCC CCGCCCGGCC 
GGTCGAGGGC ACCAGTGCCG GGGCCTCCAC CGCAAGGCCC GCAGGTGCCA GACAGAGCCC 
TGTGAAGGCT GCGAGCAGGA CGGCCGGGTC CACCGCGTGG GGGAGCGCTG GCGAGCGGGC 
CCCTGCAGGG TCTGCCAGTG TCTGCACGAC GGCTCGGCAC GCTGCTCCCC CTACTGCCCA 
CTGGGCAGCT GCCCCCAGGA CTGGGTTCTG GTGGAGGGAG TGGGGGAGTC GTGTTGCCAC 
TGCGTGCCGC CTGGAGAGAA CCAGACGGTC CACCCCATGG CCACTCCCGT GCCCGCTCCG 
ACCCCCAGCC CTCAGATCGG AGCCCCTCTG ATCACCTACC TTCTGCCTCC CCCCGGAGAT 
CCTTGCTATT CACCCCTGGG CCTGGCCCGA CTCCCCGAAG GGAGCCTGCC TGCTTCGTCC 
CAGCAGCTGG AGCACCCCGC CTGGGCTGCC ATCCTGAGGC CTGCCCCGGG GGCGCCAGGC 
TGGAGCCCCG TGGAGCACGC TGATACTCAG GGGCACACCC CGCCTCCCTA CCTGCAGCTC 
GACCTGCTCC AGCCCAGGAA CCTCACTGGC ATCATAGTGC AGGGGGCTGG GTCCTCAGAC 
TGGCTCCAGG TCAGCAGTGA TGGTCTACAC TGGCACAGCT ATCGTGACAT CCAGCATGGC 
ACTCAGCCTG CACCCCAGCT TTTCCCCAAG AACTGGAATG GCCCCTCCAC AGTGTGGATG 
TTTGCCCGGA TGGTGCAGGC GCGGCACGTC CGGGTGTGGC CTTCTGATGG GCACCACCAG 
GCTGCCCCCA GCAGTGATGC CAACCTGGAC GGCCCCCTGC GGGTGGAGCT GCTGGGCTGT 
GAGCCAGCGC CCCTGTGCCT AGGGGTTGGG CACCGTTGTG TCAGTGGCGA GTGTGCCCCC 
AGGGGGGCCC CGTGTGACGG TGTGGAGGAC TGCAAGGATG GCTCCGATGA GGAGGGCTGC 
GTAACGCCAC CCGCAGGCGC TGGCAGAATC GAGTCCACAG CCTGGAGCTC CGCGCCCTCC 
TCCGCCCAGC CTGGACAGCT GCCCCCTCAG CCCAGCGAGG GTCTGGCAGA AGCAGAAGCT 
GACCACTGGC ATCCTGGGCG GGGGTCACCC GTGCCCCCCA CAGGCAAGGG GCCAGCGAGT 
CTTGGGTCCG AGCCTCACCC AAGTCCTGGG GGATCTGTGC AGACTGTGAC CCCCACCTCC 
CAACCGGAGG CACAGGCCCT GAGACCGGAA ATGGCAGCTG TGACGGTGCT CCCCCCACAC 
CCCATGGTGA CGCCCGAGGT TCCTGCTGGA CGGAGCACCA CGCCGGGGCC CTTCCCGCAT 
GTGCAGTGCA GCCCAGGCCA GGTGCCCTGC GAGGTCCTGG GCTGCGTGGA GCTGGAGCAG 
CTGTGTGACG GGAGAGAGGA CTGTCTGGAC GGCTCTGATG AGAGGCCCTG CGCATGGGCA 
GCGGGCACCG TGCCCTTCAC CGTGCCCACC ACCACCCTGC CGGGGTTGCC GGCCTCCAGG 
GACCTCTGCT CCCCGAGCCA GCTGACCTGC GGCAGTGGGG AGTGTCTGCC TGTCGAGCGG 
CGCTGCGACC TGCAGCTGGA CTGCCAGGAC GGCTCGGACG AGAATGGCTG TGTGGACTGT 
GGCCTGGCGC CCTGGTCTGG CTGGAGCAGC TGCAGCCGTA GCTGTGGCCT GGGCCTCGCC 
TTCCAGCGCC GGGAGCTACT GCGGCCGCCC CTGCCCGGGG GCAGCTGCCC ACCGGACCGG 
CTCCGCAGCC AGCCCTGCTT CGTGCAGGCC TGCCCAGTGG CTGGGGCGTG GGCTGAGTGG 
GAGGCCTGGG GGCCCTGCAG CGTCTCGTGT GGGGGCGGCC ATCGGAGTCG TCGGAGAAGC 
TGTATGGACC CCCCGCCCAA GAATGGTGGT GCCCCCTGCC CCGGGCCCCC CCAAGAGAGG 
GCACCCTGTG GCCTGCAACC CTGTGCAGGT GGCACAGACT GCGGGCAGGG CCGAGTGCAT 
GTGAGTGCTG AGCTCTGCCG GAAGGGGCTG GTGCCACCGT GCCCACCCTC CTGCCTGGAT 
CCTGAGGCCA ACAGGAGCTG CAGCGGACTC TGTCTGGAGG GGTGTCGCTG CCCCCCCGGG 
CTCCTTCTCC AGGACGCAGG CTGCCTGCCC CTCTCCGAGT GCCCCTGCCT TGTTGGCGAA 
GAGCTGCAGC AGCCAGGCGT GCCCTTCCTC CTGGATAACT GCAGCCGATG CGTGTGTGAG 
AAGGGGGCCC TGTTGTGTGA ACCAGGGGGC TGCCCCGTGC CCTGCGGCTG GTCAGCCTGG 
TCGTCCTGGG GTCCCTGCGA CCGCTCCTGT GGCTCTGGCC TGAGGGCCCG GTTCAGGTCC 
CCCTCCAACC CTCCGGCTGC TTCGGGGGGC GCCCCGTGTG AGGGCCAGAG GCAGGAGCTG 
CAGGCCTGCT ACTCGGCGTG TGGGGCAGAG GTGCCCGGCT GGACACCCTG GGCTCCCTGG 
TCTGCCTGCT CCCAGAGCTG CCTCGTCCCT GGAGGGGGCC CAGCCCTGCG CAGCCGTTCC 
CGGCTCTGCC CCGGCCCCGG GGACACGTCC TGCATAGGAG AGGCCACCGA GGAGGAGCCC 
TGCAGCCCCC CTGTGTGCCT AGGGCTGGGT GTCTGGGGTC AGTGGGCCGC CTGGTCCGCC 
TGCTCTGCCC CATGTAATGG GGGCGTCCAG ACCCGAGGGC GCAGGTGCTC TGCCTCGGCT 
CCTGGGGACC CCGGGTGCCA GGGACCCCAC AGCCAGACCA GGGACTGCAA CACGCAGCCC 
TGCACAGCCC AGTGCCCGGG CGACATGGTG TTCCGCTCAG CAGAGCAGTG CCGCTGGGAG 
GGGGGCCCGT GCCCCGGGCT GTGCCTGGCG CGGGGCCCTG GGGTGGAGTG CACTGGCGTC 
TGCACCGCTG GCTGCGCCTG CCCTACAGGC CTCTTCCTGC ACAACAGCAG CTGCCTGCCT 
CCGAGTCAGT GCCCCTGCCA GCTGCGCGGG CAGCTCTATG CCCCCGGAGC AGTGGCCCGG 
CTGGACTCCT GCAGCAACTG CACCTGTATC TCTGGTGAGA TGGTGTGTGC CTCGGAGCCC 
TGCCCAGTGG CCTGTGGCTG GAGCCCCTGG ACCCCGTGGA GTCTCTGCAG CCGCAGCTGT 
AACGTGGGCG TTCGGCGGCG CTTCCGGGCG GGCACAGCTC CCCCTGCTGC CTTCGGGGGG 
GCTGCGTGCC AGGGCCCCAA CATGGAGGCT GAATTCTGCA GCCTGCGGCC ATGCGGAGGT 
CCTGCTGGGG AGTGGGGCCC CTGGTCTCCA TGCTCTGTGC CCTGTGGGGG CGGCTACCGG 
AATCGCACCC GAGGGAGCAG CGGGCCCAGC CCCGTGGACT TCTCCACCTG TGGTCTGCAG 
CCCTGTGCGG GGCCAGCGCC CGGTGTGTGT CCCCCGGGCA AGCGGTGGCT GGACTGTGCC 
CAGGGGCCTG CGTCCTGTGC AGAGCTCAGC GCCCCAAGAG GGGCTGACCA GCCCTGCCAC 
CCTGGCTGCT ACTGCCCTTC CGGGATGCTC CTGCTGAACA ACGCGTGCGT GCCCACCCAG 
GACTGCCCCT GCACCCATGG GGGACGCCTC CACCCCCCAG GCAGTGCTGT GCTTCGTCCA 
TGTGAGAATT GCTCCTGTGT CTCTGGGCTC ATCACTAACT GCACCTCCTG GCCCTGTAAG 
GAGGGTCAGC CCACGTGGTC ACCCTGGACC CCATGGAGCG AGTGTTCGGC CTCTTGTGGC 
CCAGCCCGGC GCCATAAACA CCGCTTCTGC ACCAGGCCCC CCGGTGGGGC ACCGTCCAGC 
ATGGCCCCGC CACTCCTGCT GTCCTCGGTG CCCCCTCTTT GCCCAGGCCC CGAGGCAGAG 
GAGGAGCCCT GCCTCCTGCC AGAGTGTGAC CGAGCTGGAG GCTGGGGCCC TTGGGGACCC 
TGGTCCAGCT GTAGCCGGAG CTGTGGAGGG GGTCTCCGGA GCCGGAGCCG AGCCTGTGAT 
CAGCCCCCAC CCCAGGGCCT GGGGGATTAC TGCGAGGGGC CTCGGGCCCA GGGGGCGGCC 
TGCCAGGCTC TGCCATGCCC AGTGACCAAC TGCACCGCCA TCGAAGGGGC GGAGTACAGC 
GCCTGTGGCC CTCCCTGCCC TCGCTCCTGC GATGACCTCG TGCACTGCGT TTGGCACTGC 
CAGCCCGGCT GTTACTGCCC CCCAGGCCAG GTGCTGAGTG CCGACGGCAC CGTCCACGTG 
CAGCCCGGTC ACTGCAGCTG CCTGGACCTG CTGACTGGGG AGCGGCACCG TCCAGGGGCC 
CAGCTGGCCA AGCCTGACGG CTGCAACTAC TGCACCTGCT CCGAGGGCCA GCTCACTTGC 
ACAGACCTGC CTTGCCCAGT GCCTGGCGCC TGGTGCCCGT GGTCAGAGTG GACAGCGTGC 
TCCCAGCCCT GCCAGGGACA GACGAGGACC CGCTCCAGGG CCTGCAGCTG CCCGGCTCCA 
CAGCATGGCG GGGCCCCATG CCCCGGGGAG GCAGGGGAGG CGGGGGCCCA GCATCAGAGG 
GAGACCTGTG CCAGCACCCC CGAGTGCCCA GTGGATGGAG CCTGGAGCCC GTGGGGACCG 
TGGTCTCCCT GTGAAGTGTG CCTGGGGCGG TCCCATCGCA GCCGAGAGTG TAGCTGGCCC 
CCCACCTCTG AGGGAGGCCG GCCCTGCCCT GGGGGCCACA GGCAGAGTCG CCCCTGCCAG 
GGCAATTCCA CTCAGTGCAC GGACTGTGCA GGTGGCCAGG ATCTTCTCCC CTGTGGGCAG 
CCCTGCCCCC GCTCCTGCGA GGACCTGTCC CCTGGGGTCG AGTGCCAGCC AGACTCGATG 
GGCTGCCAGC AGCCCCGCTG TGGGTGTCCC CCTGGCCAGC TGTCCCAGGA CGGCCTTTGC 
GTGACCCCCT CCCAGTGCCG CTGCCAGTAC CAGCCTGGAG CCATGGGGAT CCCTGAGAAC 
CAGAGCCGCT CTGCGGGGTC TGGGCTCAGC TCCTGGGAGA GCCTGGAGCC CGGTGAGGTG 
GTGACGGGGC CGTGTGACAA CTGCACCTGT GTGGCAGGCA TCCTTCAGTG CCAGGAGGTG 
CCCGCCTGCT CAGGGCTGGG GCTGTGGGGC TCCTGGGGCC CCTGGGAGGA CTGCAGTGTC 
TCCTGTGGTG GAGGGGAGCA GCTGCGCTTC CGCCGTTGCC CGCGCCCCCC CTGCCCCGGG 
CCTGCCCGCC AGAGCCGCAC CTGCCGCACC CAGGTCTGCA GAGAGGCAGG CTGCCCGGCT 
GGGCGTCTGT ACCGTGAATG TCAGCCCAGC GAAGGGTGCC CCTTCTCCTG CGCCCACGTC 
ACGGGGCAGG TGGGCTGCTT CTCCGCTGGC TGCGAGGAGG GCTGTCACTG CCCCGAGGGC 
ACCTTCCTGC ACCGTTCGGC CTGTGTCCAG GAGTGCCCCT GTGTGCTCAC CGCCTTGTGG 
CTGCAGGGGC TAGGAGCTGC CGGCGCTGAC CCGGGGGCTC ACCTGTCTGT TCTGGGAGAA 
AACGGTCAGC CTCTTGGGCC TGGCGATGAG TTGGGCTCGG GTCAGAGCCT CCGTACAGGC 
TGTCACAACT GCTCCTGTGC GCACGGGAAG CTGTCCTGCT CCGTGGAGGC CTGCTCCAAG 
GCTGCTGGTG GCTTCAGCCC CTGGGGTCCG TGGGGCCCCT GCTCCCGCTC CTGCGGCGGG 
CTGGGCACCC GCACCCGCAG CCGCCAGTGT GTGCGCCCCA TGCCCGCTCC CGGTGGCCAG 
GGCTGCCACG GGCCCCACTG GGACCTCGAG TACTGCCCCA GCCCGGAGTG CCCAGGGGCT 
GCAGGGTCCA CGGCGGAGCC AGCGACAGGC CTTCCAGGCG GCTGGGGCCT GTGGTCCCCG 
TGGTCCCCCT GCTCCGGTAC CTGCACAGAC CCGGCTCACC CCGCTTGGCG CAGCCGCAGC 
CGCCTCTGCC TGGCGAACTG CACTGGGGGT GCCGCTTCCC AGGAACGCCC CTGCAACCTG 
CCCTCCTGCA CAGAGTTGCC CCTGTGCCCG GGCCCTGGCT GCGAGGCCGG GAACTGTTCC 
TGGACTGCCT GGGCCCCGTG GGAGCCGTGC TCCCGCAGCT GTGGGGTGGG CCAGCAGCGC 
CGCCTGCGGG CCTACCACCC CCCAGGGCCC GGTGGACACT GGTGCCCTGA CGTGCTTACA 
GCCTACCAGG AACGCCGCTT CTGCAACCTG CGAGCTTGCC CAGTGCCTGG AGGCTGGTCA 
CGCTGGAGCC CCTGGTCCTG GTGCGACCGG AGCTGCGGAG GGGGCCGGTC CCTGAGGAGC 
CGCAGCTGCT CGAGCCCCCC ACCCAAGAAC GGCGGCGCCC CCTGTGTTGG GGAGAGGCAT 
CACGCACGGC TCTGCAATCC CACGCCCTGT GAGGAGGGCT GCCCTGCAGG CATGGAGGTG 
GTCAGCTGTG CCAACCGCTG CCCCCGCCGC TGCTCAGACC TCCAGGAGGG GATCGTGTGT 
CAGGAGGACC AGGCCTGCCA GCAGGGCTGC CGCTGTCCCG AGGGGTCCCT GGAGCAGGAC 
GGCGGCTGCG TGCCCCTTGG GCACTGCGAG TGCACGGATG CCCAGGGCCA CAGCTGGGCC 
CCGGGGAGCC AGCACCAGGA GGCCTGCAAC AACTGCACGT GCCGGGCGGG GCAGCTCTCC 
TGCACCGCCC AGCCCTGCCC GCCTCCTGCC CACTGCGCCT GGAGCCGCTG GTCAGCCTGG 
AGTCCCTGCA GCCGCTCGTG CGGGCCCGCA GGGCAGCAGA GCCGCTTCCG GTCTTCCACG 
TCGGGCTCGT GGGCCCCGGA GTGTCGGGAG GAGCAGTCCC AGAGCCAGCC CTGCCCTCAG 
TCCCCGTGCC CACCCCTGTG CCTGCAGGGC ACTCGCCCCC GCTCCCTGGG GGACAGCTGG 
CTGCAGGATG GATGCCAGCA GTGCTCCTGC ACCCCGGAGG GCATCATATG TGAAGACGCC 
GAGTGTGCAG GGCTCGGGGC CTGGACGCCG TGGTCCCCCT GGTCTGACTG CCCTGTCTCC 
TGTGGAGGTG GAAACCAGGT CCGCACCCGG GTCTGTGTGG CCTCGGCCCC TCCCCGAGGG 
GGCTCCCCCT GCCTGGGCCC CGATGTCCAG AGCCAGCGCT GCGGGCTGTG GCCTTGCCCG 
GCGCTGCCAG ACACCTGCTC CTGGGGCCCC TGGGGGCCCT GCTCCCGCAG CTGCGGTCCG 
GGCCTGGCCT CTCGCTCTGC GTCCTGCCCC TGCCTGCTAG CTGAGGCTGA GCCCGCCTGC 
AACAGTACCT CCCCGCGCCT GGACACCCAG GCCTGCTATG CGGGGCCCTG CCTGGAGGAG 
TGTGTGTGGA GCAGCTGGAG CAGCTGGACG CGGTGCTCAT GCGAGGTGCT GGTGCAGCAG 
CGCTACCGAC ACCAGCGGCC CGCGCCCGGG GGCGCCGGGG CGGGCCCCCC CTGCACTCGA 
CTGGACGGCC ACTTCCGGCC TTGCCTCACG GGAAACTGCT CCGAGGACAG CTGTGCGCCT 
CCCTTCGAGT TCCAGGCCTG TGGCTCCCCC TGCACTGGAC TCTGTGCCAC ATACCTGAGC 
CCTTGGCTCT GCCAGGACCT GCCGCCCTGC CAGCCTGGCT GCTACTGCCC TGAGGGGCTA 
CTGGAGCAGG CCGGAGGCTG TGTACCCCCA GAACAGTGTA ACTGCCAGCA CGTCTCAGGA 
GAAGGAGCTG GGGTGACCCT GGCCCCTGGG GACCGCCTGC AGCTGGGCTG CAAAGAGTGT 
GAATGCCAGC GCGGGGAGCT ACAGTGCACC AGCCAAGGCT GTCAAGGTCT TCTGCCTCTG 
AGCGGCTGGT CTGAGTGGTC ACCCTGTGGG CCCTGCCTGC CCCTCGGCCT GCTGGCCCCT 
GCCTCCAGGG CTGCCCTAGA GGAGCGCTGG CCCCAGGACA CAGCTGGCCT GTCCCCCACC 
TCGGCCCCCA CGCTGGCTTC GGAGCAGCAC CGCCACCGCC TCTGTTTGGA CCCTGAGACA 
GGGAGGCCGT GGGCCGGGGA CCCAGACCTC TGCACTGTGC CCCTCAGCCA ACAACGCCTC 
TGTCCAGACC CTGGAGCCTG CCAAGACTTA TGTCAGTGGG GTCCCTGGGG GGCCTGGAGC 
CCCTGCCAGG TGCCCTGCAG TGGGGGATTC CGGCTGCGCT GGAGAGAGGC CGGGATCCCC 
CCTGGAGGAG GCTGCCGGGG GCCATGGGCT CAGACTGAGA GCTGTAACAT GGGGCCTTGC 
CCAGGAGAGA GCTGCGAGGC CCAGGACACC GTGCCCACCC CTGACTGTGC CAACCAGTGC 
CCAAGAAGCT GTGTTGACCT CTGGGACCGC GTGGAGTGTC TGCAGGGACC ATGCCGCCCA 
GGCTGCCGCT GTCCCCCTGG CCAGCTGGTA CAGGATGGGC ACTGTGTGCC TGTGTCTTCT 
TGCCGTTGCG GCCTCCCCAG CCCCAACGCT TCCTGGGCGC TGGCCCCGGC TGAGGTGGTG 
CGGCTGGACT GCAGAAACTG CACCTGTGTC AACGGGTCCC TGGCGTGCTC CTCCCATGAG 
TGTCCAACCC TCGGGCCTTG GTCAGCCTGG AGCAACTGCT CTGCTCCCTG TGGTGGGGGC 
ACCACCAAAA GACATCGGAG CTGCAAGGAG GGCCCCGGGG TGACACCGTG CCAGGCCCAG 
GACATGGAGC AACAGCAGGA CTGTAACCTG CAGCCCTGCC CCGAGTGCCC ACCCGGCCAG 
GTGCTCAGTG CCTGCGCTGT CTCGTGCCCG CGCCTCTGTT CGCATCTGCA GCCTGGCACC 
CCCTGCATGC AGGAACCCTG CCAGCTTGGC TGTGACTGCC CCAGAGGGCA GCTACTGCAC 
AACGGCACGT GTGTGCCCCC TGCCGAGTGC CCCTGCACCC AGCTGTCTCT GCCCTGGGGC 
CTCACCTTGA CTCTTGAAGA GCAGCATCGA GAGCTGCCCC CAGGGACTCT GCTCACCCAG 
AACTGCACCC ACTGCATCTG CCAAGGTGGA GCCTTCAGCT GCTCCCTCAC TGACTGTCAG 
GAGTGCCCCC CCGGAGAAAC GTGGCAGCAG GTGGCCCCCG GGGAGCTGGG GCCCTGCGAG 
CAGACGTGCC GGGAACCCAA CGCCACAGAG ACCCAGGGCA ACTGCAGTGG GAGGCAGGCC 
CCGGGCTGTG TGTGCCAGCG CGGGCACTTC CGCAGCCAGG AGGGCCCCTG CGTGCCCGTG 
GATCTCTGCG AGTGCTGGCA CCATGGGCGC CCCCACCCGC CGGGATCCGA GTGGCAGAAG 
GCCTGTGAGA GCTGCCGCTG CGTCAGCGGG GAGAGTATCT GCACCCAGCA CTGCCCCCCG 
CTCACCTGTG CCCAGGGTGA GACGGCCGTG CAGGAGCCTG GAGGCTGCTG TCCCACTTGC 
CGCCAGGAGG CTCCGGAAGA GCAGCCCGTC TCCTGCAGGC ACCTCACGGA GCTTCGCAAC 
CTCACCAAGG GCGCCTGCTA CCTAGAGCAG GTGGAAGTGA ACTACTGCAG CGGGCACTGC 
CCGTCCAGCA CCAACGTCCT GCCTGAGGAG CCGTACCTGC AGAGTCAGTG TGACTGCTGT 
AGCTACCGCC TAGACCCTGA GAACCCGGTG CGCATCCTGA ACCTGCGCTG CCCTGGCGGC 
CGCACAGAGC TGGTGGTGCT GCCGGTCATT CACAGCTGCC AGTGCAGCGC CTGCCAGGGA 
GGTGATTTCT CAGAGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777131.1
CDS	154..15594
Translation	MLLPALLFGAAWALANGRWCEQTETVLVEEEVTPHQEDLVPCASLQHYQRRGWRLDLTWSGRAGLCAIYKPPETRPAAWNRTVRACCPGWGGTHCTLALAEASPEGHCFATWLCNLGAGSVNASAGSLEECCAQPWGHSWRDGRSQTCHSCSNHSRLGSTPSPAILQPLAGAVAQLWSQRQRPSATCATWSGFHYRTFDGRHFHFLGRCTYLLAGAADATWAVHLQPMGHCPQPGHCQLARVMMGPEEVLIRGENVTVNGRLVPEGASQLLPGLSLQWQGDWLVLSGGLGVVVRLDRSSSVSISVDQELQGQTQGLCGVYNGQPEDDFLEPGRGLAALAATFGNSWRLPDSELGCLDAVEAAQGCEDPLRGTETGTEAGQLRAEAQDVCHQLLEGPFRECHTQVPPAEYHEACLFAYCAGAPAGSGRAERLEAVCATLASYAQDCAARRIAVRWRKPGFCERLCPGGQLYSDCASACPPSCSAVGEGSEWSCGEECVSGCECPPGLFWDGALCVPAARCPCYRRRRRYEPGDTVRQLCNPCECRDGRWLCAQAPCAAECAVGGDGHYVTFDGRSFSFRGRAGCRFILVQDFAKRQLLIVLEHGDCDAGSCLHAISVSLGDTLVQLRDSGVVLVDGQDVALPWSAAGGLSVSRASSSFLLLRWPGARILWGVSDPAAYITLDPHHAHQVQGLCGTFTRNQQDDFLTPAGDVETSITAFASKFQVAGGGTCSLEACTPLSPCSTHTERQVFAEVACAILHGPTFQECHGLVDREAFHLRCLAAVCGCTPGRDCLCPVLAAYARRCAQEGALPSWRNQTFCPVLCPGGQEYQECAPACDRNCGEPEDCGELDNCVAGCNCPLGLLWDPEGQCVPPNLCPCQLGAHRYAPGSATMKDCNHCVCQERGLWNCTAHHCAPPRTFCPRELVYVPGACLLTCDSLDADRTCPPGSPGGCVCPPGTVLLEERCVPPELCPCRHGGQWYLPNAAIQEDCNLCVCQGQQWHCTGQRCDGRCRASGAPHYVTFDGLALTFPGACEYLLVREASGQFMVSAQNLPCGASGLTCTKALTVRLQGTVVHMLRGRAVMVNGVSVTPPKVYSGPGLSLHTAGLFLLLSTRLGLTLLWDGGTRVPVQLSPQLRGRVAGLCGDFDGDASNDLRSRQGVLEPTAELAAHSWRLGPLCPEPGDLPHPCAVNAHRAGWARARCGVVLQPLFARCHVEVPPQQHYEQCVYDACGCDSGGDCECLCSAIATYADECARHGIHVRWRSQELCPLQCERGQVYEACGPTCPATCHDHRPEPGWPCRAVACVEGCFCPEGTLLHGGVCLEPAACPCEWGGSFFPPGTVLQKDCGNNCTCRESQWLCGDDGGRCVEPGPGCAEGETPCRESGHCVPHGWLCDNQDDCGDGSDEEGCATRVCGEGQVSCCSGRCLPLVLLCDGQDDCGDGMDEQGCPCPQDSLTCADGHCLPPARLCDGHPDCPDGADEESCLGQVDCAPGEVSCVDGTCLGAIQLCDGVWDCLDGGDEGPGHCPLPSLPTPPAGTLPGPSAVSWKLHLPPWPVSALRLPCGPLDFACGSGECAPRGWRCDGEEDCADGSDESGCDRPCAPHHAPCARGSHCVAAEQLCDGVPHCPDGSDEDPGACERLQAPGGPNRTGLPCPEYSCPDGLCIGFQQVCDGQPDCELAGTAGPSPEEQGCGAWGPWSPWELCSRTCGPGVQGWSRRCSPPSLPVLWHCPGPERQTRACFAAACPEDGVWTSWSRWSPCSEPCGGVTARHRECHPPQNGGRTCATLPGGPPSTRETRPCPQDGCPNVTCSGELVFHACVPCPLTCDDISGQATCPPDRPCGGPGCWCPAGQVLGAQGRCVWPRQCPCLVDGSRYWPGQRVKTDCQLCVCQDGRPRRCQPSLDCAVNCGWSAWSPWAECLGPCGSRSVQWSFRSPNNPRPAGRGHQCRGLHRKARRCQTEPCEGCEQDGRVHRVGERWRAGPCRVCQCLHDGSARCSPYCPLGSCPQDWVLVEGVGESCCHCVPPGENQTVHPMATPVPAPTPSPQIGAPLITYLLPPPGDPCYSPLGLARLPEGSLPASSQQLEHPAWAAILRPAPGAPGWSPVEHADTQGHTPPPYLQLDLLQPRNLTGIIVQGAGSSDWLQVSSDGLHWHSYRDIQHGTQPAPQLFPKNWNGPSTVWMFARMVQARHVRVWPSDGHHQAAPSSDANLDGPLRVELLGCEPAPLCLGVGHRCVSGECAPRGAPCDGVEDCKDGSDEEGCVTPPAGAGRIESTAWSSAPSSAQPGQLPPQPSEGLAEAEADHWHPGRGSPVPPTGKGPASLGSEPHPSPGGSVQTVTPTSQPEAQALRPEMAAVTVLPPHPMVTPEVPAGRSTTPGPFPHVQCSPGQVPCEVLGCVELEQLCDGREDCLDGSDERPCAWAAGTVPFTVPTTTLPGLPASRDLCSPSQLTCGSGECLPVERRCDLQLDCQDGSDENGCVDCGLAPWSGWSSCSRSCGLGLAFQRRELLRPPLPGGSCPPDRLRSQPCFVQACPVAGAWAEWEAWGPCSVSCGGGHRSRRRSCMDPPPKNGGAPCPGPPQERAPCGLQPCAGGTDCGQGRVHVSAELCRKGLVPPCPPSCLDPEANRSCSGLCLEGCRCPPGLLLQDAGCLPLSECPCLVGEELQQPGVPFLLDNCSRCVCEKGALLCEPGGCPVPCGWSAWSSWGPCDRSCGSGLRARFRSPSNPPAASGGAPCEGQRQELQACYSACGAEVPGWTPWAPWSACSQSCLVPGGGPALRSRSRLCPGPGDTSCIGEATEEEPCSPPVCLGLGVWGQWAAWSACSAPCNGGVQTRGRRCSASAPGDPGCQGPHSQTRDCNTQPCTAQCPGDMVFRSAEQCRWEGGPCPGLCLARGPGVECTGVCTAGCACPTGLFLHNSSCLPPSQCPCQLRGQLYAPGAVARLDSCSNCTCISGEMVCASEPCPVACGWSPWTPWSLCSRSCNVGVRRRFRAGTAPPAAFGGAACQGPNMEAEFCSLRPCGGPAGEWGPWSPCSVPCGGGYRNRTRGSSGPSPVDFSTCGLQPCAGPAPGVCPPGKRWLDCAQGPASCAELSAPRGADQPCHPGCYCPSGMLLLNNACVPTQDCPCTHGGRLHPPGSAVLRPCENCSCVSGLITNCTSWPCKEGQPTWSPWTPWSECSASCGPARRHKHRFCTRPPGGAPSSMAPPLLLSSVPPLCPGPEAEEEPCLLPECDRAGGWGPWGPWSSCSRSCGGGLRSRSRACDQPPPQGLGDYCEGPRAQGAACQALPCPVTNCTAIEGAEYSACGPPCPRSCDDLVHCVWHCQPGCYCPPGQVLSADGTVHVQPGHCSCLDLLTGERHRPGAQLAKPDGCNYCTCSEGQLTCTDLPCPVPGAWCPWSEWTACSQPCQGQTRTRSRACSCPAPQHGGAPCPGEAGEAGAQHQRETCASTPECPVDGAWSPWGPWSPCEVCLGRSHRSRECSWPPTSEGGRPCPGGHRQSRPCQGNSTQCTDCAGGQDLLPCGQPCPRSCEDLSPGVECQPDSMGCQQPRCGCPPGQLSQDGLCVTPSQCRCQYQPGAMGIPENQSRSAGSGLSSWESLEPGEVVTGPCDNCTCVAGILQCQEVPACSGLGLWGSWGPWEDCSVSCGGGEQLRFRRCPRPPCPGPARQSRTCRTQVCREAGCPAGRLYRECQPSEGCPFSCAHVTGQVGCFSAGCEEGCHCPEGTFLHRSACVQECPCVLTALWLQGLGAAGADPGAHLSVLGENGQPLGPGDELGSGQSLRTGCHNCSCAHGKLSCSVEACSKAAGGFSPWGPWGPCSRSCGGLGTRTRSRQCVRPMPAPGGQGCHGPHWDLEYCPSPECPGAAGSTAEPATGLPGGWGLWSPWSPCSGTCTDPAHPAWRSRSRLCLANCTGGAASQERPCNLPSCTELPLCPGPGCEAGNCSWTAWAPWEPCSRSCGVGQQRRLRAYHPPGPGGHWCPDVLTAYQERRFCNLRACPVPGGWSRWSPWSWCDRSCGGGRSLRSRSCSSPPPKNGGAPCVGERHHARLCNPTPCEEGCPAGMEVVSCANRCPRRCSDLQEGIVCQEDQACQQGCRCPEGSLEQDGGCVPLGHCECTDAQGHSWAPGSQHQEACNNCTCRAGQLSCTAQPCPPPAHCAWSRWSAWSPCSRSCGPAGQQSRFRSSTSGSWAPECREEQSQSQPCPQSPCPPLCLQGTRPRSLGDSWLQDGCQQCSCTPEGIICEDAECAGLGAWTPWSPWSDCPVSCGGGNQVRTRVCVASAPPRGGSPCLGPDVQSQRCGLWPCPALPDTCSWGPWGPCSRSCGPGLASRSASCPCLLAEAEPACNSTSPRLDTQACYAGPCLEECVWSSWSSWTRCSCEVLVQQRYRHQRPAPGGAGAGPPCTRLDGHFRPCLTGNCSEDSCAPPFEFQACGSPCTGLCATYLSPWLCQDLPPCQPGCYCPEGLLEQAGGCVPPEQCNCQHVSGEGAGVTLAPGDRLQLGCKECECQRGELQCTSQGCQGLLPLSGWSEWSPCGPCLPLGLLAPASRAALEERWPQDTAGLSPTSAPTLASEQHRHRLCLDPETGRPWAGDPDLCTVPLSQQRLCPDPGACQDLCQWGPWGAWSPCQVPCSGGFRLRWREAGIPPGGGCRGPWAQTESCNMGPCPGESCEAQDTVPTPDCANQCPRSCVDLWDRVECLQGPCRPGCRCPPGQLVQDGHCVPVSSCRCGLPSPNASWALAPAEVVRLDCRNCTCVNGSLACSSHECPTLGPWSAWSNCSAPCGGGTTKRHRSCKEGPGVTPCQAQDMEQQQDCNLQPCPECPPGQVLSACAVSCPRLCSHLQPGTPCMQEPCQLGCDCPRGQLLHNGTCVPPAECPCTQLSLPWGLTLTLEEQHRELPPGTLLTQNCTHCICQGGAFSCSLTDCQECPPGETWQQVAPGELGPCEQTCREPNATETQGNCSGRQAPGCVCQRGHFRSQEGPCVPVDLCECWHHGRPHPPGSEWQKACESCRCVSGESICTQHCPPLTCAQGETAVQEPGGCCPTCRQEAPEEQPVSCRHLTELRNLTKGACYLEQVEVNYCSGHCPSSTNVLPEEPYLQSQCDCCSYRLDPENPVRILNLRCPGGRTELVVLPVIHSCQCSACQGGDFSER

Target ORF information:

RefSeq Version	NM_174706.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SCO-spondin (SSPO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174706.2

ORF Insert Sequence:

ATGCTGCTCC CTGCCCTCCT CTTCGGGGCG GCATGGGCAC TGGCTAATGG GCGGTGGTGT 
GAGCAGACAG AGACCGTCCT GGTGGAGGAG GAAGTCACCC CCCATCAGGA GGACCTGGTG 
CCCTGTGCCA GCCTCCAGCA TTACCAGCGC CGGGGCTGGC GGCTGGACCT GACCTGGAGT 
GGCCGTGCGG GGCTCTGTGC CATCTACAAA CCGCCAGAGA CCCGGCCTGC TGCCTGGAAC 
CGGACAGTGA GGGCCTGCTG CCCAGGCTGG GGGGGCACCC ACTGCACCCT AGCCCTTGCA 
GAGGCCAGCC CTGAGGGCCA CTGCTTTGCC ACCTGGCTGT GCAACCTGGG GGCAGGCTCA 
GTGAATGCGT CTGCAGGAAG TCTGGAGGAG TGCTGTGCCC AACCCTGGGG ACACAGCTGG 
CGGGATGGCC GTTCCCAGAC CTGCCACAGC TGCTCCAACC ACAGTCGACT GGGCAGCACA 
CCCTCCCCAG CCATCCTGCA GCCCCTGGCG GGGGCTGTGG CCCAGCTCTG GAGCCAGCGC 
CAGCGTCCCT CGGCCACCTG CGCCACCTGG TCCGGCTTCC ACTATCGCAC CTTCGATGGA 
CGCCACTTCC ACTTCCTGGG CCGCTGCACC TACCTGCTGG CGGGTGCGGC TGATGCCACC 
TGGGCCGTCC ACCTCCAGCC CATGGGGCAT TGTCCCCAGC CTGGGCACTG TCAGCTGGCC 
CGGGTAATGA TGGGACCAGA GGAGGTGCTG ATCCGGGGTG AAAACGTCAC TGTGAACGGG 
CGGCTGGTAC CCGAGGGGGC GTCTCAGCTG CTCCCTGGGC TCAGCCTGCA GTGGCAAGGG 
GACTGGCTGG TGCTGTCGGG AGGCCTGGGG GTCGTTGTGC GGCTGGACCG GTCCAGTTCC 
GTCTCCATTT CTGTGGACCA GGAGCTCCAG GGACAGACTC AAGGCCTCTG TGGGGTCTAC 
AACGGCCAGC CTGAGGATGA CTTCCTAGAG CCTGGCAGGG GACTGGCTGC GTTAGCTGCC 
ACCTTTGGGA ATTCCTGGAG ACTCCCAGAC TCAGAGCTTG GGTGTCTGGA TGCGGTGGAG 
GCAGCTCAAG GCTGTGAGGA CCCCCTGAGG GGCACAGAGA CGGGCACGGA GGCTGGCCAG 
CTTCGGGCTG AAGCCCAGGA TGTGTGCCAC CAGCTGCTGG AAGGCCCATT CAGAGAATGT 
CACACCCAGG TGCCCCCTGC AGAGTACCAC GAGGCCTGCC TCTTCGCCTA CTGTGCTGGG 
GCCCCAGCGG GCAGTGGGCG GGCAGAGCGG CTGGAGGCTG TGTGCGCCAC CCTGGCCAGC 
TACGCCCAGG ACTGTGCGGC TAGGCGCATC GCGGTGCGCT GGAGGAAGCC GGGCTTCTGC 
GAGCGCCTGT GTCCGGGGGG CCAGCTGTAC TCCGACTGCG CCTCGGCCTG CCCGCCCAGC 
TGCTCGGCAG TGGGGGAGGG AAGCGAATGG AGCTGCGGGG AAGAATGCGT GAGCGGCTGC 
GAGTGCCCGC CGGGTCTCTT CTGGGACGGC GCCCTGTGCG TGCCCGCCGC CCGCTGCCCC 
TGCTACCGCC GACGCCGGCG CTACGAGCCC GGGGACACCG TGCGCCAGCT CTGCAACCCG 
TGCGAGTGCA GGGACGGCCG CTGGCTCTGC GCGCAGGCGC CGTGCGCCGC CGAGTGCGCG 
GTAGGCGGGG ACGGGCATTA CGTCACCTTC GATGGGCGGA GCTTCTCCTT CCGCGGCCGC 
GCCGGTTGCC GCTTCATCCT GGTGCAGGAC TTTGCCAAGC GGCAGCTGCT GATTGTACTG 
GAACACGGGG ACTGTGACGC TGGGAGCTGC CTCCATGCCA TCTCTGTCTC CCTGGGGGAC 
ACCCTCGTCC AGCTCAGGGA CTCAGGAGTT GTGCTGGTCG ACGGTCAGGA TGTGGCCTTG 
CCCTGGAGTG CAGCTGGGGG CCTCAGTGTC TCCCGGGCCT CTTCCTCTTT CCTGCTGCTG 
CGCTGGCCTG GTGCCCGCAT CCTCTGGGGA GTGTCTGACC CTGCAGCCTA CATCACCCTG 
GACCCCCACC ACGCCCACCA GGTGCAGGGT CTGTGTGGCA CCTTCACCCG GAATCAGCAG 
GATGACTTCC TGACCCCTGC TGGCGATGTG GAGACTAGTA TCACCGCCTT TGCTAGCAAG 
TTCCAGGTGG CAGGCGGGGG AACGTGCTCC TTGGAGGCCT GCACTCCGCT TTCCCCCTGC 
AGCACCCACA CTGAGCGCCA GGTTTTCGCG GAGGTAGCCT GTGCCATCCT GCATGGCCCA 
ACCTTCCAGG AGTGCCACGG GCTGGTGGAC AGGGAGGCGT TCCACCTGCG CTGCTTGGCA 
GCTGTGTGTG GCTGCACCCC TGGCCGGGAC TGCCTGTGTC CCGTGCTTGC GGCCTATGCT 
CGGCGCTGTG CCCAGGAGGG TGCCCTGCCT TCCTGGAGAA ACCAGACCTT CTGCCCTGTC 
CTGTGTCCTG GTGGCCAGGA GTACCAGGAG TGCGCCCCAG CATGCGATCG CAACTGCGGG 
GAGCCTGAAG ACTGTGGGGA GCTGGACAAC TGTGTGGCCG GCTGTAATTG CCCCCTAGGG 
CTGCTGTGGG ACCCCGAGGG CCAGTGTGTG CCCCCCAACT TGTGCCCCTG CCAGCTTGGG 
GCCCATCGCT ATGCCCCTGG CAGTGCTACT ATGAAGGACT GCAACCACTG TGTCTGCCAG 
GAGAGGGGCC TCTGGAACTG CACTGCTCAC CACTGTGCTC CGCCCCGGAC CTTCTGCCCC 
CGTGAGCTTG TCTATGTCCC TGGAGCCTGC CTCCTCACCT GTGACAGCCT TGATGCCGAC 
CGCACCTGCC CCCCAGGCAG CCCGGGGGGC TGTGTCTGCC CCCCAGGCAC CGTGCTGCTG 
GAGGAACGCT GCGTGCCTCC TGAGCTCTGT CCCTGCCGTC ATGGCGGCCA GTGGTACCTG 
CCCAATGCTG CAATCCAGGA GGACTGCAAC CTGTGCGTGT GCCAGGGTCA GCAGTGGCAC 
TGCACGGGCC AGCGGTGCGA TGGACGGTGC CGGGCGTCAG GCGCCCCTCA CTATGTGACG 
TTCGACGGGC TGGCCCTCAC CTTCCCCGGG GCCTGCGAGT ACCTGCTGGT GCGAGAGGCC 
AGTGGCCAGT TCATGGTCTC CGCCCAAAAC CTGCCGTGCG GAGCCAGTGG CCTCACCTGC 
ACCAAGGCGC TGACTGTGCG GCTGCAGGGC ACCGTTGTGC ACATGCTCAG AGGCAGGGCA 
GTGATGGTGA ATGGGGTGAG TGTGACGCCC CCCAAGGTCT ACTCAGGCCC CGGGCTGAGC 
CTGCACACCG CCGGCCTCTT CCTGCTTCTC AGCACCCGCC TGGGCCTCAC CTTGCTCTGG 
GATGGAGGCA CCCGGGTCCC GGTGCAGCTG TCCCCCCAGC TCCGCGGCAG GGTGGCTGGC 
CTGTGTGGAG ACTTCGATGG AGATGCCAGT AACGACCTTC GGAGCCGCCA GGGGGTCCTG 
GAGCCCACAG CTGAACTGGC CGCCCACTCC TGGCGCCTCG GTCCTCTCTG CCCAGAGCCA 
GGGGACCTGC CACATCCCTG TGCAGTGAAT GCCCACCGGG CTGGCTGGGC CCGCGCCCGC 
TGCGGGGTGG TGCTGCAGCC GCTCTTTGCC CGGTGCCACG TGGAGGTGCC CCCGCAGCAG 
CACTACGAGC AGTGTGTGTA TGATGCCTGC GGCTGCGATT CGGGGGGCGA CTGTGAGTGT 
CTCTGCTCGG CCATTGCCAC CTATGCAGAT GAGTGTGCCC GGCACGGGAT CCACGTGCGC 
TGGCGCAGCC AGGAGCTCTG CCCTCTGCAG TGTGAGAGGG GCCAGGTGTA TGAGGCCTGT 
GGCCCCACGT GCCCCGCCAC CTGCCACGAC CACCGTCCTG AGCCTGGGTG GCCCTGCCGG 
GCCGTCGCCT GTGTGGAGGG CTGCTTCTGC CCTGAGGGGA CTCTGTTGCA TGGAGGTGTC 
TGCTTGGAGC CGGCTGCCTG CCCCTGCGAG TGGGGGGGCA GCTTCTTCCC ACCGGGCACG 
GTGCTGCAGA AGGACTGTGG CAACAACTGC ACGTGTCGGG AAAGTCAGTG GCTTTGTGGG 
GACGATGGCG GCCGCTGTGT GGAGCCCGGG CCTGGTTGTG CGGAGGGAGA GACCCCATGC 
CGCGAGAGCG GGCACTGTGT GCCCCACGGG TGGCTCTGTG ACAACCAGGA TGACTGTGGC 
GATGGCTCAG ACGAGGAGGG CTGTGCCACC CGGGTCTGTG GCGAGGGGCA GGTGTCTTGC 
TGCTCCGGCC GCTGCCTGCC CCTGGTCCTG CTCTGTGATG GGCAGGACGA CTGTGGAGAC 
GGGATGGATG AGCAGGGCTG CCCGTGCCCC CAGGACTCGC TGACCTGTGC CGACGGGCAC 
TGCCTGCCCC CGGCCCGGCT CTGTGACGGG CACCCCGACT GTCCGGATGG TGCTGACGAG 
GAGTCTTGCC TGGGGCAAGT GGACTGCGCC CCTGGGGAGG TGTCCTGCGT CGATGGTACC 
TGCCTGGGGG CCATCCAGCT GTGTGATGGA GTCTGGGACT GTCTGGATGG TGGCGATGAG 
GGGCCTGGGC ACTGCCCCCT CCCCTCTCTG CCCACTCCTC CTGCTGGTAC CTTGCCTGGC 
CCCTCTGCTG TCTCCTGGAA ACTGCACCTA CCCCCCTGGC CAGTGTCGGC CCTGCGCTTG 
CCCTGCGGCC CCTTGGACTT CGCGTGCGGC AGCGGCGAGT GCGCGCCCCG GGGCTGGCGC 
TGCGACGGCG AGGAGGACTG CGCCGACGGC AGCGACGAGA GCGGCTGCGA CCGGCCCTGC 
GCGCCGCACC ACGCGCCCTG CGCGCGCGGC TCGCACTGCG TGGCCGCGGA GCAGCTGTGC 
GACGGCGTCC CGCATTGCCC GGACGGCTCA GACGAGGACC CGGGCGCCTG CGAGAGGCTG 
CAGGCCCCAG GAGGCCCCAA CCGGACAGGG CTTCCCTGCC CAGAATACTC CTGCCCGGAT 
GGCCTCTGCA TCGGTTTCCA GCAGGTGTGC GATGGGCAGC CCGACTGTGA GCTGGCGGGG 
ACAGCAGGGC CCTCCCCAGA GGAGCAGGGC TGTGGGGCCT GGGGGCCCTG GAGCCCCTGG 
GAGCTGTGCA GCCGGACATG TGGGCCCGGG GTGCAGGGCT GGAGTCGCCG CTGCTCGCCC 
CCGAGCCTCC CGGTGCTGTG GCACTGCCCC GGCCCAGAGC GCCAGACTCG GGCCTGCTTC 
GCGGCCGCCT GCCCAGAGGA CGGTGTATGG ACCTCCTGGT CGCGCTGGTC TCCATGCTCT 
GAGCCATGCG GGGGCGTCAC GGCTCGCCAC CGGGAGTGCC ATCCGCCCCA GAATGGTGGC 
CGGACCTGTG CCACGCTGCC TGGGGGCCCT CCCAGCACCC GGGAGACCCG GCCCTGCCCT 
CAGGATGGCT GCCCCAACGT CACCTGCTCT GGGGAGCTGG TGTTCCATGC CTGTGTTCCT 
TGCCCTTTGA CCTGTGATGA CATCTCTGGT CAGGCCACGT GCCCCCCCGA CCGGCCCTGT 
GGTGGCCCAG GCTGCTGGTG CCCTGCGGGG CAGGTGCTGG GTGCCCAGGG GCGGTGCGTG 
TGGCCACGGC AATGCCCCTG CCTGGTGGAC GGCAGCCGCT ACTGGCCCGG GCAGCGTGTC 
AAGACTGATT GCCAGCTCTG CGTCTGCCAG GACGGGCGGC CCCGGCGCTG CCAGCCCAGC 
CTAGACTGTG CAGTGAACTG CGGCTGGTCA GCGTGGTCCC CCTGGGCTGA GTGCCTGGGC 
CCCTGTGGCA GCCGGAGCGT CCAGTGGTCC TTCCGGAGCC CCAACAACCC CCGCCCGGCC 
GGTCGAGGGC ACCAGTGCCG GGGCCTCCAC CGCAAGGCCC GCAGGTGCCA GACAGAGCCC 
TGTGAAGGCT GCGAGCAGGA CGGCCGGGTC CACCGCGTGG GGGAGCGCTG GCGAGCGGGC 
CCCTGCAGGG TCTGCCAGTG TCTGCACGAC GGCTCGGCAC GCTGCTCCCC CTACTGCCCA 
CTGGGCAGCT GCCCCCAGGA CTGGGTTCTG GTGGAGGGAG TGGGGGAGTC GTGTTGCCAC 
TGCGTGCCGC CTGGAGAGAA CCAGACGGTC CACCCCATGG CCACTCCCGT GCCCGCTCCG 
ACCCCCAGCC CTCAGATCGG AGCCCCTCTG ATCACCTACC TTCTGCCTCC CCCCGGAGAT 
CCTTGCTATT CACCCCTGGG CCTGGCCCGA CTCCCCGAAG GGAGCCTGCC TGCTTCGTCC 
CAGCAGCTGG AGCACCCCGC CTGGGCTGCC ATCCTGAGGC CTGCCCCGGG GGCGCCAGGC 
TGGAGCCCCG TGGAGCACGC TGATACTCAG GGGCACACCC CGCCTCCCTA CCTGCAGCTC 
GACCTGCTCC AGCCCAGGAA CCTCACTGGC ATCATAGTGC AGGGGGCTGG GTCCTCAGAC 
TGGCTCCAGG TCAGCAGTGA TGGTCTACAC TGGCACAGCT ATCGTGACAT CCAGCATGGC 
ACTCAGCCTG CACCCCAGCT TTTCCCCAAG AACTGGAATG GCCCCTCCAC AGTGTGGATG 
TTTGCCCGGA TGGTGCAGGC GCGGCACGTC CGGGTGTGGC CTTCTGATGG GCACCACCAG 
GCTGCCCCCA GCAGTGATGC CAACCTGGAC GGCCCCCTGC GGGTGGAGCT GCTGGGCTGT 
GAGCCAGCGC CCCTGTGCCT AGGGGTTGGG CACCGTTGTG TCAGTGGCGA GTGTGCCCCC 
AGGGGGGCCC CGTGTGACGG TGTGGAGGAC TGCAAGGATG GCTCCGATGA GGAGGGCTGC 
GTAACGCCAC CCGCAGGCGC TGGCAGAATC GAGTCCACAG CCTGGAGCTC CGCGCCCTCC 
TCCGCCCAGC CTGGACAGCT GCCCCCTCAG CCCAGCGAGG GTCTGGCAGA AGCAGAAGCT 
GACCACTGGC ATCCTGGGCG GGGGTCACCC GTGCCCCCCA CAGGCAAGGG GCCAGCGAGT 
CTTGGGTCCG AGCCTCACCC AAGTCCTGGG GGATCTGTGC AGACTGTGAC CCCCACCTCC 
CAACCGGAGG CACAGGCCCT GAGACCGGAA ATGGCAGCTG TGACGGTGCT CCCCCCACAC 
CCCATGGTGA CGCCCGAGGT TCCTGCTGGA CGGAGCACCA CGCCGGGGCC CTTCCCGCAT 
GTGCAGTGCA GCCCAGGCCA GGTGCCCTGC GAGGTCCTGG GCTGCGTGGA GCTGGAGCAG 
CTGTGTGACG GGAGAGAGGA CTGTCTGGAC GGCTCTGATG AGAGGCCCTG CGCATGGGCA 
GCGGGCACCG TGCCCTTCAC CGTGCCCACC ACCACCCTGC CGGGGTTGCC GGCCTCCAGG 
GACCTCTGCT CCCCGAGCCA GCTGACCTGC GGCAGTGGGG AGTGTCTGCC TGTCGAGCGG 
CGCTGCGACC TGCAGCTGGA CTGCCAGGAC GGCTCGGACG AGAATGGCTG TGTGGACTGT 
GGCCTGGCGC CCTGGTCTGG CTGGAGCAGC TGCAGCCGTA GCTGTGGCCT GGGCCTCGCC 
TTCCAGCGCC GGGAGCTACT GCGGCCGCCC CTGCCCGGGG GCAGCTGCCC ACCGGACCGG 
CTCCGCAGCC AGCCCTGCTT CGTGCAGGCC TGCCCAGTGG CTGGGGCGTG GGCTGAGTGG 
GAGGCCTGGG GGCCCTGCAG CGTCTCGTGT GGGGGCGGCC ATCGGAGTCG TCGGAGAAGC 
TGTATGGACC CCCCGCCCAA GAATGGTGGT GCCCCCTGCC CCGGGCCCCC CCAAGAGAGG 
GCACCCTGTG GCCTGCAACC CTGTGCAGGT GGCACAGACT GCGGGCAGGG CCGAGTGCAT 
GTGAGTGCTG AGCTCTGCCG GAAGGGGCTG GTGCCACCGT GCCCACCCTC CTGCCTGGAT 
CCTGAGGCCA ACAGGAGCTG CAGCGGACTC TGTCTGGAGG GGTGTCGCTG CCCCCCCGGG 
CTCCTTCTCC AGGACGCAGG CTGCCTGCCC CTCTCCGAGT GCCCCTGCCT TGTTGGCGAA 
GAGCTGCAGC AGCCAGGCGT GCCCTTCCTC CTGGATAACT GCAGCCGATG CGTGTGTGAG 
AAGGGGGCCC TGTTGTGTGA ACCAGGGGGC TGCCCCGTGC CCTGCGGCTG GTCAGCCTGG 
TCGTCCTGGG GTCCCTGCGA CCGCTCCTGT GGCTCTGGCC TGAGGGCCCG GTTCAGGTCC 
CCCTCCAACC CTCCGGCTGC TTCGGGGGGC GCCCCGTGTG AGGGCCAGAG GCAGGAGCTG 
CAGGCCTGCT ACTCGGCGTG TGGGGCAGAG GTGCCCGGCT GGACACCCTG GGCTCCCTGG 
TCTGCCTGCT CCCAGAGCTG CCTCGTCCCT GGAGGGGGCC CAGCCCTGCG CAGCCGTTCC 
CGGCTCTGCC CCGGCCCCGG GGACACGTCC TGCATAGGAG AGGCCACCGA GGAGGAGCCC 
TGCAGCCCCC CTGTGTGCCT AGGGCTGGGT GTCTGGGGTC AGTGGGCCGC CTGGTCCGCC 
TGCTCTGCCC CATGTAATGG GGGCGTCCAG ACCCGAGGGC GCAGGTGCTC TGCCTCGGCT 
CCTGGGGACC CCGGGTGCCA GGGACCCCAC AGCCAGACCA GGGACTGCAA CACGCAGCCC 
TGCACAGCCC AGTGCCCGGG CGACATGGTG TTCCGCTCAG CAGAGCAGTG CCGCTGGGAG 
GGGGGCCCGT GCCCCGGGCT GTGCCTGGCG CGGGGCCCTG GGGTGGAGTG CACTGGCGTC 
TGCACCGCTG GCTGCGCCTG CCCTACAGGC CTCTTCCTGC ACAACAGCAG CTGCCTGCCT 
CCGAGTCAGT GCCCCTGCCA GCTGCGCGGG CAGCTCTATG CCCCCGGAGC AGTGGCCCGG 
CTGGACTCCT GCAGCAACTG CACCTGTATC TCTGGTGAGA TGGTGTGTGC CTCGGAGCCC 
TGCCCAGTGG CCTGTGGCTG GAGCCCCTGG ACCCCGTGGA GTCTCTGCAG CCGCAGCTGT 
AACGTGGGCG TTCGGCGGCG CTTCCGGGCG GGCACAGCTC CCCCTGCTGC CTTCGGGGGG 
GCTGCGTGCC AGGGCCCCAA CATGGAGGCT GAATTCTGCA GCCTGCGGCC ATGCGGAGGT 
CCTGCTGGGG AGTGGGGCCC CTGGTCTCCA TGCTCTGTGC CCTGTGGGGG CGGCTACCGG 
AATCGCACCC GAGGGAGCAG CGGGCCCAGC CCCGTGGACT TCTCCACCTG TGGTCTGCAG 
CCCTGTGCGG GGCCAGCGCC CGGTGTGTGT CCCCCGGGCA AGCGGTGGCT GGACTGTGCC 
CAGGGGCCTG CGTCCTGTGC AGAGCTCAGC GCCCCAAGAG GGGCTGACCA GCCCTGCCAC 
CCTGGCTGCT ACTGCCCTTC CGGGATGCTC CTGCTGAACA ACGCGTGCGT GCCCACCCAG 
GACTGCCCCT GCACCCATGG GGGACGCCTC CACCCCCCAG GCAGTGCTGT GCTTCGTCCA 
TGTGAGAATT GCTCCTGTGT CTCTGGGCTC ATCACTAACT GCACCTCCTG GCCCTGTAAG 
GAGGGTCAGC CCACGTGGTC ACCCTGGACC CCATGGAGCG AGTGTTCGGC CTCTTGTGGC 
CCAGCCCGGC GCCATAAACA CCGCTTCTGC ACCAGGCCCC CCGGTGGGGC ACCGTCCAGC 
ATGGCCCCGC CACTCCTGCT GTCCTCGGTG CCCCCTCTTT GCCCAGGCCC CGAGGCAGAG 
GAGGAGCCCT GCCTCCTGCC AGAGTGTGAC CGAGCTGGAG GCTGGGGCCC TTGGGGACCC 
TGGTCCAGCT GTAGCCGGAG CTGTGGAGGG GGTCTCCGGA GCCGGAGCCG AGCCTGTGAT 
CAGCCCCCAC CCCAGGGCCT GGGGGATTAC TGCGAGGGGC CTCGGGCCCA GGGGGCGGCC 
TGCCAGGCTC TGCCATGCCC AGTGACCAAC TGCACCGCCA TCGAAGGGGC GGAGTACAGC 
GCCTGTGGCC CTCCCTGCCC TCGCTCCTGC GATGACCTCG TGCACTGCGT TTGGCACTGC 
CAGCCCGGCT GTTACTGCCC CCCAGGCCAG GTGCTGAGTG CCGACGGCAC CGTCCACGTG 
CAGCCCGGTC ACTGCAGCTG CCTGGACCTG CTGACTGGGG AGCGGCACCG TCCAGGGGCC 
CAGCTGGCCA AGCCTGACGG CTGCAACTAC TGCACCTGCT CCGAGGGCCA GCTCACTTGC 
ACAGACCTGC CTTGCCCAGT GCCTGGCGCC TGGTGCCCGT GGTCAGAGTG GACAGCGTGC 
TCCCAGCCCT GCCAGGGACA GACGAGGACC CGCTCCAGGG CCTGCAGCTG CCCGGCTCCA 
CAGCATGGCG GGGCCCCATG CCCCGGGGAG GCAGGGGAGG CGGGGGCCCA GCATCAGAGG 
GAGACCTGTG CCAGCACCCC CGAGTGCCCA GTGGATGGAG CCTGGAGCCC GTGGGGACCG 
TGGTCTCCCT GTGAAGTGTG CCTGGGGCGG TCCCATCGCA GCCGAGAGTG TAGCTGGCCC 
CCCACCTCTG AGGGAGGCCG GCCCTGCCCT GGGGGCCACA GGCAGAGTCG CCCCTGCCAG 
GGCAATTCCA CTCAGTGCAC GGACTGTGCA GGTGGCCAGG ATCTTCTCCC CTGTGGGCAG 
CCCTGCCCCC GCTCCTGCGA GGACCTGTCC CCTGGGGTCG AGTGCCAGCC AGACTCGATG 
GGCTGCCAGC AGCCCCGCTG TGGGTGTCCC CCTGGCCAGC TGTCCCAGGA CGGCCTTTGC 
GTGACCCCCT CCCAGTGCCG CTGCCAGTAC CAGCCTGGAG CCATGGGGAT CCCTGAGAAC 
CAGAGCCGCT CTGCGGGGTC TGGGCTCAGC TCCTGGGAGA GCCTGGAGCC CGGTGAGGTG 
GTGACGGGGC CGTGTGACAA CTGCACCTGT GTGGCAGGCA TCCTTCAGTG CCAGGAGGTG 
CCCGCCTGCT CAGGGCTGGG GCTGTGGGGC TCCTGGGGCC CCTGGGAGGA CTGCAGTGTC 
TCCTGTGGTG GAGGGGAGCA GCTGCGCTTC CGCCGTTGCC CGCGCCCCCC CTGCCCCGGG 
CCTGCCCGCC AGAGCCGCAC CTGCCGCACC CAGGTCTGCA GAGAGGCAGG CTGCCCGGCT 
GGGCGTCTGT ACCGTGAATG TCAGCCCAGC GAAGGGTGCC CCTTCTCCTG CGCCCACGTC 
ACGGGGCAGG TGGGCTGCTT CTCCGCTGGC TGCGAGGAGG GCTGTCACTG CCCCGAGGGC 
ACCTTCCTGC ACCGTTCGGC CTGTGTCCAG GAGTGCCCCT GTGTGCTCAC CGCCTTGTGG 
CTGCAGGGGC TAGGAGCTGC CGGCGCTGAC CCGGGGGCTC ACCTGTCTGT TCTGGGAGAA 
AACGGTCAGC CTCTTGGGCC TGGCGATGAG TTGGGCTCGG GTCAGAGCCT CCGTACAGGC 
TGTCACAACT GCTCCTGTGC GCACGGGAAG CTGTCCTGCT CCGTGGAGGC CTGCTCCAAG 
GCTGCTGGTG GCTTCAGCCC CTGGGGTCCG TGGGGCCCCT GCTCCCGCTC CTGCGGCGGG 
CTGGGCACCC GCACCCGCAG CCGCCAGTGT GTGCGCCCCA TGCCCGCTCC CGGTGGCCAG 
GGCTGCCACG GGCCCCACTG GGACCTCGAG TACTGCCCCA GCCCGGAGTG CCCAGGGGCT 
GCAGGGTCCA CGGCGGAGCC AGCGACAGGC CTTCCAGGCG GCTGGGGCCT GTGGTCCCCG 
TGGTCCCCCT GCTCCGGTAC CTGCACAGAC CCGGCTCACC CCGCTTGGCG CAGCCGCAGC 
CGCCTCTGCC TGGCGAACTG CACTGGGGGT GCCGCTTCCC AGGAACGCCC CTGCAACCTG 
CCCTCCTGCA CAGAGTTGCC CCTGTGCCCG GGCCCTGGCT GCGAGGCCGG GAACTGTTCC 
TGGACTGCCT GGGCCCCGTG GGAGCCGTGC TCCCGCAGCT GTGGGGTGGG CCAGCAGCGC 
CGCCTGCGGG CCTACCACCC CCCAGGGCCC GGTGGACACT GGTGCCCTGA CGTGCTTACA 
GCCTACCAGG AACGCCGCTT CTGCAACCTG CGAGCTTGCC CAGTGCCTGG AGGCTGGTCA 
CGCTGGAGCC CCTGGTCCTG GTGCGACCGG AGCTGCGGAG GGGGCCGGTC CCTGAGGAGC 
CGCAGCTGCT CGAGCCCCCC ACCCAAGAAC GGCGGCGCCC CCTGTGTTGG GGAGAGGCAT 
CACGCACGGC TCTGCAATCC CACGCCCTGT GAGGAGGGCT GCCCTGCAGG CATGGAGGTG 
GTCAGCTGTG CCAACCGCTG CCCCCGCCGC TGCTCAGACC TCCAGGAGGG GATCGTGTGT 
CAGGAGGACC AGGCCTGCCA GCAGGGCTGC CGCTGTCCCG AGGGGTCCCT GGAGCAGGAC 
GGCGGCTGCG TGCCCCTTGG GCACTGCGAG TGCACGGATG CCCAGGGCCA CAGCTGGGCC 
CCGGGGAGCC AGCACCAGGA GGCCTGCAAC AACTGCACGT GCCGGGCGGG GCAGCTCTCC 
TGCACCGCCC AGCCCTGCCC GCCTCCTGCC CACTGCGCCT GGAGCCGCTG GTCAGCCTGG 
AGTCCCTGCA GCCGCTCGTG CGGGCCCGCA GGGCAGCAGA GCCGCTTCCG GTCTTCCACG 
TCGGGCTCGT GGGCCCCGGA GTGTCGGGAG GAGCAGTCCC AGAGCCAGCC CTGCCCTCAG 
TCCCCGTGCC CACCCCTGTG CCTGCAGGGC ACTCGCCCCC GCTCCCTGGG GGACAGCTGG 
CTGCAGGATG GATGCCAGCA GTGCTCCTGC ACCCCGGAGG GCATCATATG TGAAGACGCC 
GAGTGTGCAG GGCTCGGGGC CTGGACGCCG TGGTCCCCCT GGTCTGACTG CCCTGTCTCC 
TGTGGAGGTG GAAACCAGGT CCGCACCCGG GTCTGTGTGG CCTCGGCCCC TCCCCGAGGG 
GGCTCCCCCT GCCTGGGCCC CGATGTCCAG AGCCAGCGCT GCGGGCTGTG GCCTTGCCCG 
GCGCTGCCAG ACACCTGCTC CTGGGGCCCC TGGGGGCCCT GCTCCCGCAG CTGCGGTCCG 
GGCCTGGCCT CTCGCTCTGC GTCCTGCCCC TGCCTGCTAG CTGAGGCTGA GCCCGCCTGC 
AACAGTACCT CCCCGCGCCT GGACACCCAG GCCTGCTATG CGGGGCCCTG CCTGGAGGAG 
TGTGTGTGGA GCAGCTGGAG CAGCTGGACG CGGTGCTCAT GCGAGGTGCT GGTGCAGCAG 
CGCTACCGAC ACCAGCGGCC CGCGCCCGGG GGCGCCGGGG CGGGCCCCCC CTGCACTCGA 
CTGGACGGCC ACTTCCGGCC TTGCCTCACG GGAAACTGCT CCGAGGACAG CTGTGCGCCT 
CCCTTCGAGT TCCAGGCCTG TGGCTCCCCC TGCACTGGAC TCTGTGCCAC ATACCTGAGC 
CCTTGGCTCT GCCAGGACCT GCCGCCCTGC CAGCCTGGCT GCTACTGCCC TGAGGGGCTA 
CTGGAGCAGG CCGGAGGCTG TGTACCCCCA GAACAGTGTA ACTGCCAGCA CGTCTCAGGA 
GAAGGAGCTG GGGTGACCCT GGCCCCTGGG GACCGCCTGC AGCTGGGCTG CAAAGAGTGT 
GAATGCCAGC GCGGGGAGCT ACAGTGCACC AGCCAAGGCT GTCAAGGTCT TCTGCCTCTG 
AGCGGCTGGT CTGAGTGGTC ACCCTGTGGG CCCTGCCTGC CCCTCGGCCT GCTGGCCCCT 
GCCTCCAGGG CTGCCCTAGA GGAGCGCTGG CCCCAGGACA CAGCTGGCCT GTCCCCCACC 
TCGGCCCCCA CGCTGGCTTC GGAGCAGCAC CGCCACCGCC TCTGTTTGGA CCCTGAGACA 
GGGAGGCCGT GGGCCGGGGA CCCAGACCTC TGCACTGTGC CCCTCAGCCA ACAACGCCTC 
TGTCCAGACC CTGGAGCCTG CCAAGACTTA TGTCAGTGGG GTCCCTGGGG GGCCTGGAGC 
CCCTGCCAGG TGCCCTGCAG TGGGGGATTC CGGCTGCGCT GGAGAGAGGC CGGGATCCCC 
CCTGGAGGAG GCTGCCGGGG GCCATGGGCT CAGACTGAGA GCTGTAACAT GGGGCCTTGC 
CCAGGAGAGA GCTGCGAGGC CCAGGACACC GTGCCCACCC CTGACTGTGC CAACCAGTGC 
CCAAGAAGCT GTGTTGACCT CTGGGACCGC GTGGAGTGTC TGCAGGGACC ATGCCGCCCA 
GGCTGCCGCT GTCCCCCTGG CCAGCTGGTA CAGGATGGGC ACTGTGTGCC TGTGTCTTCT 
TGCCGTTGCG GCCTCCCCAG CCCCAACGCT TCCTGGGCGC TGGCCCCGGC TGAGGTGGTG 
CGGCTGGACT GCAGAAACTG CACCTGTGTC AACGGGTCCC TGGCGTGCTC CTCCCATGAG 
TGTCCAACCC TCGGGCCTTG GTCAGCCTGG AGCAACTGCT CTGCTCCCTG TGGTGGGGGC 
ACCACCAAAA GACATCGGAG CTGCAAGGAG GGCCCCGGGG TGACACCGTG CCAGGCCCAG 
GACATGGAGC AACAGCAGGA CTGTAACCTG CAGCCCTGCC CCGAGTGCCC ACCCGGCCAG 
GTGCTCAGTG CCTGCGCTGT CTCGTGCCCG CGCCTCTGTT CGCATCTGCA GCCTGGCACC 
CCCTGCATGC AGGAACCCTG CCAGCTTGGC TGTGACTGCC CCAGAGGGCA GCTACTGCAC 
AACGGCACGT GTGTGCCCCC TGCCGAGTGC CCCTGCACCC AGCTGTCTCT GCCCTGGGGC 
CTCACCTTGA CTCTTGAAGA GCAGCATCGA GAGCTGCCCC CAGGGACTCT GCTCACCCAG 
AACTGCACCC ACTGCATCTG CCAAGGTGGA GCCTTCAGCT GCTCCCTCAC TGACTGTCAG 
GAGTGCCCCC CCGGAGAAAC GTGGCAGCAG GTGGCCCCCG GGGAGCTGGG GCCCTGCGAG 
CAGACGTGCC GGGAACCCAA CGCCACAGAG ACCCAGGGCA ACTGCAGTGG GAGGCAGGCC 
CCGGGCTGTG TGTGCCAGCG CGGGCACTTC CGCAGCCAGG AGGGCCCCTG CGTGCCCGTG 
GATCTCTGCG AGTGCTGGCA CCATGGGCGC CCCCACCCGC CGGGATCCGA GTGGCAGAAG 
GCCTGTGAGA GCTGCCGCTG CGTCAGCGGG GAGAGTATCT GCACCCAGCA CTGCCCCCCG 
CTCACCTGTG CCCAGGGTGA GACGGCCGTG CAGGAGCCTG GAGGCTGCTG TCCCACTTGC 
CGCCAGGAGG CTCCGGAAGA GCAGCCCGTC TCCTGCAGGC ACCTCACGGA GCTTCGCAAC 
CTCACCAAGG GCGCCTGCTA CCTAGAGCAG GTGGAAGTGA ACTACTGCAG CGGGCACTGC 
CCGTCCAGCA CCAACGTCCT GCCTGAGGAG CCGTACCTGC AGAGTCAGTG TGACTGCTGT 
AGCTACCGCC TAGACCCTGA GAACCCGGTG CGCATCCTGA ACCTGCGCTG CCCTGGCGGC 
CGCACAGAGC TGGTGGTGCT GCCGGTCATT CACAGCTGCC AGTGCAGCGC CTGCCAGGGA 
GGTGATTTCT CAGAGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The lengthening of a giant protein: when, how, and why?
Journal of molecular evolution66(1)1-10(2008 Jan)
Meiniel O,Meiniel R,Lallou? F,Didier R,Jauberteau MO,Meiniel A,Petit D

The complex multidomain organization of SCO-spondin protein is highly conserved in mammals.
Brain research reviews53(2)321-7(2007 Feb)
Meiniel O,Meiniel A

Transcription of SCO-spondin in the subcommissural organ: evidence for down-regulation mediated by serotonin.
Brain research. Molecular brain research129(1-2)151-62(2004 Oct)
Richter HG,Tom? MM,Yulis CR,V?o KJ,Jim?nez AJ,P?rez-F?gares JM,Rodr?guez EM

Bovine floor plate explants secrete SCO-spondin.
Cell and tissue research308(2)177-91(2002 May)
Gui?az? MF,Richter HG,Rodr?guez EM

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SSPO cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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