PPARG cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPARG
Entrez Gene ID	281993
Full Name	peroxisome proliferator activated receptor gamma
General protein information	Preferred Names peroxisome proliferator activated receptor gamma Names peroxisome proliferator-activated receptor gamma PPAR-gamma nuclear receptor subfamily 1 group C member 3 peroxisome proliferative activated receptor, gamma peroxisome proliferator activated receptor gamma 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 22 Map Location: 22q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_181024.2	NP_851367.1	peroxisome proliferator-activated receptor gamma

Pathways from BioSystems

Homologies

	PPARG	NP_001028032.1
Rattus norvegicus (Norway rat)	Pparg	NP_037256.1
Pan troglodytes (chimpanzee)	PPARG	XP_001153669.1
Danio rerio (zebrafish)	pparg	NP_571542.1
Homo sapiens (human)	PPARG	NP_056953.2
Canis lupus familiaris (dog)	PPARG	NP_001019803.1
Bos taurus (cattle)	PPARG	NP_851367.1
Mus musculus (house mouse)	Pparg	NP_035276.2
Gallus gallus (chicken)	PPARG	NP_001001460.1

Gene Ontology
Bibliography

Related articles in PubMed

Two Novel SNPs of PPAR? Significantly Affect Weaning Growth Traits of Nanyang Cattle.
Huang J, Chen N, Li X, An S, Zhao M, Sun T, Hao R, Ma Y
Animal biotechnology29(1)68-74(2018 Jan)

Docosahexaenoic acid attenuates LPS-stimulated inflammatory response by regulating the PPAR?/NF-?B pathways in primary bovine mammary epithelial cells.
He X, Liu W, Shi M, Yang Z, Zhang X, Gong P
Research in veterinary science1127-12(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicated that docosahexaenoic acid may attenuate lipopolysaccharide-stimulated inflammatory response in bovine mammary epithelial cells by suppressing NF-kappaB activation through a mechanism partly dependent on PPARgamma activation.
Title: Docosahexaenoic acid attenuates LPS-stimulated inflammatory response by regulating the PPAR?/NF-?B pathways in primary bovine mammary epithelial cells.

PPARgamma is a positive regulator of milk fat synthesis in dairy cow mammary epithelial cells.
Title: Regulation of peroxisome proliferator-activated receptor gamma on milk fat synthesis in dairy cow mammary epithelial cells.

upregulation of PPARgamma was observed in the backfat tissue of Lilu cattle with increasing age
Title: Gene expression profiling of SIRT1, FoxO1, and PPAR? in backfat tissues and subcutaneous adipocytes of Lilu bulls.

An Asp7Gly substitution in PPARG is associated with adiposity.
Title: An Asp7Gly substitution in PPARG is associated with decreased transcriptional activation activity.

A potential association of an single nucleotide polymorphism (72472 GT in exon7) of the bovine PPAR-gamma gene with carcass and meat quality traits, was evaluated.
Title: A missense mutant of the PPAR-? gene associated with carcass and meat quality traits in Chinese cattle breeds.

The following PPARG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPARG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43369	NM_181024.2 Latest version!	Bos taurus peroxisome proliferator activated receptor gamma (PPARG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPARG ( NM_181024.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_851367.1 Bos taurus peroxisome proliferator activated receptor gamma (PPARG), mRNA.

Price & Availability↑

CloneID	OBa43369
Clone ID Related Accession (Same CDS sequence)	NM_181024.2
Accession Version	NM_181024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	peroxisome proliferator-activated receptor gamma
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y12420.1. On Jun 3, 2003 this sequence version replaced NM_181024.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y12420.1, AY179866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGAAA CTCTGGGAGA TGCTCTTATT GACCCAGAGA GTGAGCCCTT CGCTGTCACA 
GTGTCTGCAA GGACCTCACA AGAAATTACC ATGGTTGACA CAGAGATGCC GTTTTGGCCC 
ACCAACTTTG GGATCAGCTC CGTGGACCTT TCTATGATGG ATGACCACTC CCATGCCTTT 
GACATCAAGC CCTTCACCAC CGTTGACTTC TCCAGCATTT CCACTCCGCA CTATGAGGAC 
ATTCCGTTCC CAAGAGCTGA CCCGATGGTT GCAGATTATA AGTATGACCT GAAGCTCCAA 
GAGTACCAAA GTGCAATCAA AGTGGAGCCT GTATCCCCAC CTTATTATTC TGAAAAGACT 
CAGCTGTACA GTAAGCCTCA TGAAGAGCCT TCCAACTCCC TCATGGCCAT TGAATGCCGG 
GTCTGTGGGG ATAAAGCGTC AGGGTTCCAC TATGGAGTTC ATGCTTGTGA AGGATGCAAG 
GGTTTCTTCC GGAGGACAAT CAGATTGAAA CTTATTTACG ATAGGTGTGA TCTTAACTGT 
CGGATCCACA AGAAAAGTAG AAATAAATGT CAGTACTGTC GGTTTCAGAA GTGCCTTGCT 
GTGGGGATGT CTCATAATGC CATCAGGTTT GGGCGCATGC CACAGGCCGA GAAGGAGAAG 
CTGTTAGCGG AGATCTCCAG CGATATCGAC CAACTGAACC CAGAGTCTGC TGACCTCCGG 
GCCCTGGCAA AGCATTTGTA TGACTCGTAC ATAAAGTCCT TCCCGCTGAC CAAAGCAAAG 
GCGAGGGCGA TCTTGACGGG AAAGACGACA GACAAATCAC CGTTTGTTAT CTATGACATG 
AACTCCTTAA TGATGGGAGA AGATAAAATC AAGTTCAAGC ACATCAGTCC CCTGCAGGAG 
CCCAGCAAAG AGGTGGCCAT CCGCATCTTC CAGGGGTGTC AGTTTCGCTC CGTGGAAGCC 
GTGCAGGAGA TCACAGAGTA CGCCAAGAAT ATCCCCGGCT TTGTGAACCT TGACTTGAAC 
GACCAAGTAA CTCTCCTAAA ATACGGCGTG CACGAGATCA TTTACACGAT GCTGGCCTCC 
TTGATGAATA AGGATGGGGT CCTCATATCC GAGGGCCAAG GATTCATGAC AAGGGAGTTT 
CTAAAGAGCC TGCGAAAGCC CTTTGGTGAC TTTATGGAGC CCAAGTTCGA GTTTGCTGTG 
AAGTTCAACG CACTGGAATT AGATGACAGC GACTTAGCAA TATTTATAGC TGTCATTATT 
CTCAGTGGAG ACCGCCCAGG TTTGCTGAAC GTGAAGCCCA TTGAGGACAT ACAAGACAAT 
CTGCTGCAAG CCTTGGAGCT GCAGCTCAAG CTGAACCACC CCGAGTCCTC CCAGCTCTTT 
GCCAAGCTGC TCCAGAAAAT GACAGACCTC AGACAGATTG TGACAGAACA CGTGCAGCTG 
TTGCAAGTAA TAAAGAAAAC AGAGACGGAC ATGAGTCTCC ACCCACTCCT ACAGGAAATC 
TACAAGGACT TGTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851367.1
CDS	180..1697
Translation	MGETLGDALIDPESEPFAVTVSARTSQEITMVDTEMPFWPTNFGISSVDLSMMDDHSHAFDIKPFTTVDFSSISTPHYEDIPFPRADPMVADYKYDLKLQEYQSAIKVEPVSPPYYSEKTQLYSKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHISPLQEPSKEVAIRIFQGCQFRSVEAVQEITEYAKNIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY

Target ORF information:

RefSeq Version	NM_181024.2
Organism	Bos taurus(cattle)
Definition	Bos taurus peroxisome proliferator activated receptor gamma (PPARG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181024.2

ORF Insert Sequence:

ATGGGTGAAA CTCTGGGAGA TGCTCTTATT GACCCAGAGA GTGAGCCCTT CGCTGTCACA 
GTGTCTGCAA GGACCTCACA AGAAATTACC ATGGTTGACA CAGAGATGCC GTTTTGGCCC 
ACCAACTTTG GGATCAGCTC CGTGGACCTT TCTATGATGG ATGACCACTC CCATGCCTTT 
GACATCAAGC CCTTCACCAC CGTTGACTTC TCCAGCATTT CCACTCCGCA CTATGAGGAC 
ATTCCGTTCC CAAGAGCTGA CCCGATGGTT GCAGATTATA AGTATGACCT GAAGCTCCAA 
GAGTACCAAA GTGCAATCAA AGTGGAGCCT GTATCCCCAC CTTATTATTC TGAAAAGACT 
CAGCTGTACA GTAAGCCTCA TGAAGAGCCT TCCAACTCCC TCATGGCCAT TGAATGCCGG 
GTCTGTGGGG ATAAAGCGTC AGGGTTCCAC TATGGAGTTC ATGCTTGTGA AGGATGCAAG 
GGTTTCTTCC GGAGGACAAT CAGATTGAAA CTTATTTACG ATAGGTGTGA TCTTAACTGT 
CGGATCCACA AGAAAAGTAG AAATAAATGT CAGTACTGTC GGTTTCAGAA GTGCCTTGCT 
GTGGGGATGT CTCATAATGC CATCAGGTTT GGGCGCATGC CACAGGCCGA GAAGGAGAAG 
CTGTTAGCGG AGATCTCCAG CGATATCGAC CAACTGAACC CAGAGTCTGC TGACCTCCGG 
GCCCTGGCAA AGCATTTGTA TGACTCGTAC ATAAAGTCCT TCCCGCTGAC CAAAGCAAAG 
GCGAGGGCGA TCTTGACGGG AAAGACGACA GACAAATCAC CGTTTGTTAT CTATGACATG 
AACTCCTTAA TGATGGGAGA AGATAAAATC AAGTTCAAGC ACATCAGTCC CCTGCAGGAG 
CCCAGCAAAG AGGTGGCCAT CCGCATCTTC CAGGGGTGTC AGTTTCGCTC CGTGGAAGCC 
GTGCAGGAGA TCACAGAGTA CGCCAAGAAT ATCCCCGGCT TTGTGAACCT TGACTTGAAC 
GACCAAGTAA CTCTCCTAAA ATACGGCGTG CACGAGATCA TTTACACGAT GCTGGCCTCC 
TTGATGAATA AGGATGGGGT CCTCATATCC GAGGGCCAAG GATTCATGAC AAGGGAGTTT 
CTAAAGAGCC TGCGAAAGCC CTTTGGTGAC TTTATGGAGC CCAAGTTCGA GTTTGCTGTG 
AAGTTCAACG CACTGGAATT AGATGACAGC GACTTAGCAA TATTTATAGC TGTCATTATT 
CTCAGTGGAG ACCGCCCAGG TTTGCTGAAC GTGAAGCCCA TTGAGGACAT ACAAGACAAT 
CTGCTGCAAG CCTTGGAGCT GCAGCTCAAG CTGAACCACC CCGAGTCCTC CCAGCTCTTT 
GCCAAGCTGC TCCAGAAAAT GACAGACCTC AGACAGATTG TGACAGAACA CGTGCAGCTG 
TTGCAAGTAA TAAAGAAAAC AGAGACGGAC ATGAGTCTCC ACCCACTCCT ACAGGAAATC 
TACAAGGACT TGTATTAG