MMP13 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MMP13
Entrez Gene ID	281914
Full Name	matrix metallopeptidase 13
General protein information	Preferred Names matrix metallopeptidase 13 Names collagenase 3 MMP-13 matrix metalloproteinase 13 (collagenase 3) matrix metalloproteinase-13
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174389.2	NP_776814.1	collagenase 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MMP13	XP_001154361.1
Danio rerio (zebrafish)	mmp13b	XP_001345507.1
Gallus gallus (chicken)	MMP13	XP_003640635.1
Xenopus tropicalis (tropical clawed frog)	LOC100490019	XP_002934971.1
Canis lupus familiaris (dog)	MMP13	XP_536598.3
Mus musculus (house mouse)	Mmp13	NP_032633.1
Homo sapiens (human)	MMP13	NP_002418.1
	MMP13	XP_001098996.1
Bos taurus (cattle)	MMP13	NP_776814.1
Rattus norvegicus (Norway rat)	Mmp13	NP_598214.1
Caenorhabditis elegans (roundworm)	CELE_C31B8.8	NP_503790.1

Gene Ontology
Bibliography

Related articles in PubMed

Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants.
Nakagawa K, Teramura T, Takehara T, Onodera Y, Hamanishi C, Akagi M, Fukuda K
Inflammation research : official journal of the European Histamine Research Society ... [et al.]61(10)1093-100(2012 Oct)

Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction.
Litherland GJ, Elias MS, Hui W, Macdonald CD, Catterall JB, Barter MJ, Farren MJ, Jefferson M, Rowan AD
The Journal of biological chemistry285(29)22414-25(2010 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

[Type II collagen fragment capacity to induce collagen cleavage and hypertrophy of articular chondrocyte].
Chetkina EV, Pul AR
Vestnik Rossiiskoi akademii meditsinskikh nauk(5)15-21(2008)

Chondrocyte hypertrophy can be induced by a cryptic sequence of type II collagen and is accompanied by the induction of MMP-13 and collagenase activity: implications for development and arthritis.
Tchetina EV, Kobayashi M, Yasuda T, Meijers T, Pidoux I, Poole AR
Matrix biology : journal of the International Society for Matrix Biology26(4)247-58(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

p38 phosphorylation and MMP13 expression are regulated by Rho/ROCK activation, and support the potential novel pathway that Rho/ROCK is in the upper part of the mechanical stress-induced matrix degeneration cascade in cartilage.
Title: Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants.

These data identify atypical PKC isozymes as STAT and ERK activators that mediate c-fos and collagenase expression.
Title: Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction.

At high but naturally occurring concentrations the collagen peptide CB12-II induced an increase in the expressions of MMP-13 and cleavage of type II collagen by collagenase in the mid zone and also in the superficial zone.
Title: [Type II collagen fragment capacity to induce collagen cleavage and hypertrophy of articular chondrocyte].

MMP-13 and collagenase have roles in chondrocyte hypertrophy induced by type II collagen
Title: Chondrocyte hypertrophy can be induced by a cryptic sequence of type II collagen and is accompanied by the induction of MMP-13 and collagenase activity: implications for development and arthritis.

involvement of p38 MAP kinase in the hyaluronan oligosaccharide induction of MMP-13
Title: Hyaluronan oligosaccharides induce matrix metalloproteinase 13 via transcriptional activation of NFkappaB and p38 MAP kinase in articular chondrocytes.

The following MMP13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41375	NM_174389.2 Latest version!	Bos taurus matrix metallopeptidase 13 (MMP13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MMP13 ( NM_174389.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776814.1 Bos taurus matrix metallopeptidase 13 (MMP13), mRNA.

Price & Availability↑

CloneID	OBa41375
Clone ID Related Accession (Same CDS sequence)	NM_174389.2
Accession Version	NM_174389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	collagenase 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF072685.1. On Jun 3, 2003 this sequence version replaced NM_174389.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145467, SAMN03145477 [ECO:0000348] ##Evidence-Data-END##

ATGCACCCAA GGGTCCTGGC TGGCTTCCTC TTCTTCAGCT GGACGGCTTG TTGGTCTCTG 
CCCCTTCCCA GTGATGGAGA TTCTGAAGAC TTGTCCGAGG AAGACTTCCA GTTTGCAGAG 
AGCTACCTGA AATCATACTA CTATCCTCAG AATCCTGCTG GAATCCTGAA GAAAACTGCA 
GCAAGCTCTG TGATTGACAG GCTTAGAGAA ATGCAGTCAT TTTTTGGCTT AGAGGTGACT 
GGCAGACTTG ATGATAACAC CTTAGACATC ATGAAAAAAC CAAGATGTGG GGTCCCTGAT 
GTGGGTGAAT ACAATGTTTT TCCTCGAACT CTCAAGTGGT CCAAAATGAA CTTAACCTAC 
AGAATTGTGA ATTATACACC TGATCTGACT CATTCTGAAG TGGAAAAGGC CTTCAGAAAA 
GCCTTCAAGG TGTGGTCTGA TGTGACCCCT CTGAATTTTA CCAGAATTCA CAATGGCACT 
GCTGATATCA TGATCTCTTT TGGAACTAAA GAGCATGGTG ACTTCTACCC ATTTGATGGA 
CCCTCTGGTC TGTTGGCTCA CGCTTTCCCT CCTGGACCAA ATTATGGAGG AGATGCTCAT 
TTTGATGATG ATGAAACCTG GACAAGTAGT TCCAAAGGCT ACAACTTGTT TCTTGTTGCT 
GCCCATGAGT TTGGCCATTC CTTAGGATTG GACCACTCCA AGGACCCAGG AGCACTCATG 
TTTCCCATCT ACACCTACAC TGGCAAAAGC CACTTTATGC TTCCTGATGA TGATGTCCAA 
GGGATTCAGT CTCTCTATGG TCCAGGAGAT GAAGACCCCT ACTCTAAACA TCCCAAAACG 
CCAGACAAAT GTGACCCTTC CTTATCCCTT GATGCCATAA CCAGTCTCCG CGGAGAAACA 
CTGATCTTTA AAGACAGATT CTTCTGGCGG CTGCATCCTC AGCAGGTTGA AGCAGAGCTG 
TTTTTAACAA AATCGTTTGG GCCAGAACTT CCCAACCGTA TTGATGCCGC CTATGAGCAC 
CCTTCCCATG ACCTTATCTT CATCTTTAGA GGCAGAAAAT TTTGGGCACT TAGTGGTTAT 
GATATTCTGG AAGATTACCC AAAAAAAATA TCTGAACTGG GATTTCCAAA ACACGTTAAA 
AAGATCAGCG CAGCCCTTCA CTTTGAGGAT TCAGGGAAGA CGCTCTTCTT TTCAGAAAAC 
CAAGTCTGGA GCTATGATGA CACTAACCAT GTGATGGATA AAGACTACCC GAGACTCATT 
GAAGAGGTCT TCCCAGGAAT TGGTGATAAA GTAGATGCTG TCTACCAGAA AAATGGTTAT 
ATCTATTTTT TCAATGGACC CATACAGTTT GAATACAGCA TCTGGAGTAA CCGTATTGTT 
CGAGTCATGA CAACAAATTC CTTATTGTGG TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776814.1
CDS	1..1416
Translation	MHPRVLAGFLFFSWTACWSLPLPSDGDSEDLSEEDFQFAESYLKSYYYPQNPAGILKKTAASSVIDRLREMQSFFGLEVTGRLDDNTLDIMKKPRCGVPDVGEYNVFPRTLKWSKMNLTYRIVNYTPDLTHSEVEKAFRKAFKVWSDVTPLNFTRIHNGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPYSKHPKTPDKCDPSLSLDAITSLRGETLIFKDRFFWRLHPQQVEAELFLTKSFGPELPNRIDAAYEHPSHDLIFIFRGRKFWALSGYDILEDYPKKISELGFPKHVKKISAALHFEDSGKTLFFSENQVWSYDDTNHVMDKDYPRLIEEVFPGIGDKVDAVYQKNGYIYFFNGPIQFEYSIWSNRIVRVMTTNSLLWC

Target ORF information:

RefSeq Version	NM_174389.2
Organism	Bos taurus(cattle)
Definition	Bos taurus matrix metallopeptidase 13 (MMP13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174389.2

ORF Insert Sequence:

ATGCACCCAA GGGTCCTGGC TGGCTTCCTC TTCTTCAGCT GGACGGCTTG TTGGTCTCTG 
CCCCTTCCCA GTGATGGAGA TTCTGAAGAC TTGTCCGAGG AAGACTTCCA GTTTGCAGAG 
AGCTACCTGA AATCATACTA CTATCCTCAG AATCCTGCTG GAATCCTGAA GAAAACTGCA 
GCAAGCTCTG TGATTGACAG GCTTAGAGAA ATGCAGTCAT TTTTTGGCTT AGAGGTGACT 
GGCAGACTTG ATGATAACAC CTTAGACATC ATGAAAAAAC CAAGATGTGG GGTCCCTGAT 
GTGGGTGAAT ACAATGTTTT TCCTCGAACT CTCAAGTGGT CCAAAATGAA CTTAACCTAC 
AGAATTGTGA ATTATACACC TGATCTGACT CATTCTGAAG TGGAAAAGGC CTTCAGAAAA 
GCCTTCAAGG TGTGGTCTGA TGTGACCCCT CTGAATTTTA CCAGAATTCA CAATGGCACT 
GCTGATATCA TGATCTCTTT TGGAACTAAA GAGCATGGTG ACTTCTACCC ATTTGATGGA 
CCCTCTGGTC TGTTGGCTCA CGCTTTCCCT CCTGGACCAA ATTATGGAGG AGATGCTCAT 
TTTGATGATG ATGAAACCTG GACAAGTAGT TCCAAAGGCT ACAACTTGTT TCTTGTTGCT 
GCCCATGAGT TTGGCCATTC CTTAGGATTG GACCACTCCA AGGACCCAGG AGCACTCATG 
TTTCCCATCT ACACCTACAC TGGCAAAAGC CACTTTATGC TTCCTGATGA TGATGTCCAA 
GGGATTCAGT CTCTCTATGG TCCAGGAGAT GAAGACCCCT ACTCTAAACA TCCCAAAACG 
CCAGACAAAT GTGACCCTTC CTTATCCCTT GATGCCATAA CCAGTCTCCG CGGAGAAACA 
CTGATCTTTA AAGACAGATT CTTCTGGCGG CTGCATCCTC AGCAGGTTGA AGCAGAGCTG 
TTTTTAACAA AATCGTTTGG GCCAGAACTT CCCAACCGTA TTGATGCCGC CTATGAGCAC 
CCTTCCCATG ACCTTATCTT CATCTTTAGA GGCAGAAAAT TTTGGGCACT TAGTGGTTAT 
GATATTCTGG AAGATTACCC AAAAAAAATA TCTGAACTGG GATTTCCAAA ACACGTTAAA 
AAGATCAGCG CAGCCCTTCA CTTTGAGGAT TCAGGGAAGA CGCTCTTCTT TTCAGAAAAC 
CAAGTCTGGA GCTATGATGA CACTAACCAT GTGATGGATA AAGACTACCC GAGACTCATT 
GAAGAGGTCT TCCCAGGAAT TGGTGATAAA GTAGATGCTG TCTACCAGAA AAATGGTTAT 
ATCTATTTTT TCAATGGACC CATACAGTTT GAATACAGCA TCTGGAGTAA CCGTATTGTT 
CGAGTCATGA CAACAAATTC CTTATTGTGG TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]61(10)1093-100(2012 Oct)
Nakagawa K,Teramura T,Takehara T,Onodera Y,Hamanishi C,Akagi M,Fukuda K

Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction.
The Journal of biological chemistry285(29)22414-25(2010 Jul)
Litherland GJ,Elias MS,Hui W,Macdonald CD,Catterall JB,Barter MJ,Farren MJ,Jefferson M,Rowan AD

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

[Type II collagen fragment capacity to induce collagen cleavage and hypertrophy of articular chondrocyte].
Vestnik Rossiiskoi akademii meditsinskikh nauk(5)15-21(2008)
Chetkina EV,Pul AR

Chondrocyte hypertrophy can be induced by a cryptic sequence of type II collagen and is accompanied by the induction of MMP-13 and collagenase activity: implications for development and arthritis.
Matrix biology : journal of the International Society for Matrix Biology26(4)247-58(2007 May)
Tchetina EV,Kobayashi M,Yasuda T,Meijers T,Pidoux I,Poole AR