FGF2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FGF2
Entrez Gene ID	281161
Full Name	fibroblast growth factor 2
Synonyms	BFGF,FGF-2,HBGF-2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174056.4	NP_776481.2	fibroblast growth factor 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FGF2	NP_001103711.1
Canis lupus familiaris (dog)	FGF2	XP_003432529.2
Homo sapiens (human)	FGF2	NP_001997.5
Rattus norvegicus (Norway rat)	Fgf2	NP_062178.1
Xenopus tropicalis (tropical clawed frog)	fgf2	NP_001017333.1
Gallus gallus (chicken)	FGF2	NP_990764.1
Danio rerio (zebrafish)	fgf2	NP_997988.1
	FGF2	XP_001099284.1
Bos taurus (cattle)	FGF2	NP_776481.1
Mus musculus (house mouse)	Fgf2	NP_032032.1

Gene Ontology
Bibliography

Related articles in PubMed

Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy.
Lim W, Bae H, Bazer FW, Song G
Biology of reproduction96(1)185-198(2017 01)

Staphylococcus aureus induces TGF-?1 and bFGF expression through the activation of AP-1 and NF-?B transcription factors in bovine mammary gland fibroblasts.
Wu J, Ding Y, Bi Y, Wang Y, Zhi Y, Wang J, Wang F
Microbial pathogenesis957-14(2016 Jun)

Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.
Farberov S, Meidan R
Biology of reproduction91(3)58(2014 Sep)

Fibroblast growth factor 2 is a key determinant of vascular sprouting during bovine luteal angiogenesis.
Woad KJ, Hunter MG, Mann GE, Laird M, Hammond AJ, Robinson RS
Reproduction (Cambridge, England)143(1)35-43(2012 Jan)

Fibroblast growth factors activate mitogen-activated protein kinase pathways to promote migration in ovine trophoblast cells.
Yang QE, Giassetti MI, Ealy AD
Reproduction (Cambridge, England)141(5)707-14(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

activation of FGFR1 and FGFR2 by uterine- and endometrial-derived FGF2 stimulates PI3K/AKT and mitogen-activated protein kinase pathways for development of the porcine uterus and improvement of litter size
Title: Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy.

Taken together, Staphylococcus aureus induces TGF-beta1 and bFGF expression through the activation of AP-1 and NF-kappaB in bovine mammary gland fibroblasts.
Title: Staphylococcus aureus induces TGF-?1 and bFGF expression through the activation of AP-1 and NF-?B transcription factors in bovine mammary gland fibroblasts.

Data suggest THBS1 (thrombospondin-1) expression predominates in luteal endothelial cells; THBS2 expression predominates in luteinized granulosa cells. Luteinizing signals down-regulate expression of THBS1/THBS2 but up-regulate expression of FGF2.
Title: Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.

Mixed populations of luteal cells were treated with SU1498 (VEGF receptor 2 inhibitor) or SU5402 (FGF receptor 1 inhibitor) or control on days 0-3, 3-6 or 6-9 to determine the role of FGF2 and VEGFA during these specific windows.
Title: Fibroblast growth factor 2 is a key determinant of vascular sprouting during bovine luteal angiogenesis.

FGF2 and FGF10 regulate migratory activity of ovine trophoblast cells through MAPK-dependent pathways.
Title: Fibroblast growth factors activate mitogen-activated protein kinase pathways to promote migration in ovine trophoblast cells.

The following FGF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa95419	NM_174056.4 Latest version!	Bos taurus fibroblast growth factor 2 (FGF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FGF2 ( NM_174056.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776481.2 Bos taurus fibroblast growth factor 2 (FGF2), mRNA.

Price & Availability↑

CloneID	OBa95419
Clone ID Related Accession (Same CDS sequence)	NM_174056.4
Accession Version	NM_174056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	fibroblast growth factor 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02044761.1. On Oct 2, 2015 this sequence version replaced NM_174056.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M13440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG GGAGCATCAC CACGCTGCCA TCCCTGCCGG AGGACGGCGG CAGCGGCGCT 
TTCCCGCCGG GCCACTTCAA GGACCCCAAG CGGCTGTACT GCAAGAACGG GGGCTTCTTC 
CTGCGCATCC ACCCCGACGG CCGAGTGGAC GGGGTCCGCG AGAAGAGCGA CCCACACATC 
AAACTACAAC TTCAAGCAGA AGAGAGAGGG GTTGTGTCTA TCAAAGGAGT GTGTGCAAAC 
CGTTACCTTG CTATGAAAGA AGATGGAAGA TTACTAGCTT CTAAATGTGT TACAGACGAG 
TGTTTCTTTT TTGAACGATT GGAGTCTAAT AACTACAATA CTTACCGGTC AAGGAAATAC 
TCCAGTTGGT ATGTGGCACT GAAACGAACT GGGCAGTATA AACTTGGACC CAAAACAGGA 
CCTGGGCAGA AAGCTATACT TTTTCTTCCA ATGTCTGCTA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776481.2
CDS	104..571
Translation	MAAGSITTLPSLPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGPKTGPGQKAILFLPMSAKS

Target ORF information:

RefSeq Version	NM_174056.4
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast growth factor 2 (FGF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174056.4

ORF Insert Sequence:

ATGGCCGCCG GGAGCATCAC CACGCTGCCA TCCCTGCCGG AGGACGGCGG CAGCGGCGCT 
TTCCCGCCGG GCCACTTCAA GGACCCCAAG CGGCTGTACT GCAAGAACGG GGGCTTCTTC 
CTGCGCATCC ACCCCGACGG CCGAGTGGAC GGGGTCCGCG AGAAGAGCGA CCCACACATC 
AAACTACAAC TTCAAGCAGA AGAGAGAGGG GTTGTGTCTA TCAAAGGAGT GTGTGCAAAC 
CGTTACCTTG CTATGAAAGA AGATGGAAGA TTACTAGCTT CTAAATGTGT TACAGACGAG 
TGTTTCTTTT TTGAACGATT GGAGTCTAAT AACTACAATA CTTACCGGTC AAGGAAATAC 
TCCAGTTGGT ATGTGGCACT GAAACGAACT GGGCAGTATA AACTTGGACC CAAAACAGGA 
CCTGGGCAGA AAGCTATACT TTTTCTTCCA ATGTCTGCTA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy.
Biology of reproduction96(1)185-198(2017 01)
Lim W,Bae H,Bazer FW,Song G

Staphylococcus aureus induces TGF-?1 and bFGF expression through the activation of AP-1 and NF-?B transcription factors in bovine mammary gland fibroblasts.
Microbial pathogenesis957-14(2016 Jun)
Wu J,Ding Y,Bi Y,Wang Y,Zhi Y,Wang J,Wang F

Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.
Biology of reproduction91(3)58(2014 Sep)
Farberov S,Meidan R

Fibroblast growth factor 2 is a key determinant of vascular sprouting during bovine luteal angiogenesis.
Reproduction (Cambridge, England)143(1)35-43(2012 Jan)
Woad KJ,Hunter MG,Mann GE,Laird M,Hammond AJ,Robinson RS

Fibroblast growth factors activate mitogen-activated protein kinase pathways to promote migration in ovine trophoblast cells.
Reproduction (Cambridge, England)141(5)707-14(2011 May)
Yang QE,Giassetti MI,Ealy AD

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FGF2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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