CCN2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCN2
Entrez Gene ID	281103
Full Name	connective tissue growth factor
General protein information	Preferred Names connective tissue growth factor Names connective tissue growth factor CCN family member 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174030.2	NP_776455.1	CCN family member 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CTGF	XP_518744.2
Canis lupus familiaris (dog)	LOC476202	XP_003638817.1
Mus musculus (house mouse)	Ctgf	NP_034347.2
Gallus gallus (chicken)	CTGF	NP_989605.1
Xenopus tropicalis (tropical clawed frog)	ctgf	NP_001015042.1
Rattus norvegicus (Norway rat)	Ctgf	NP_071602.1
Homo sapiens (human)	CTGF	NP_001892.1
Bos taurus (cattle)	CTGF	NP_776455.1
Danio rerio (zebrafish)	ctgfa	NP_001015041.1

Gene Ontology
Bibliography

Related articles in PubMed

Actions of activin A, connective tissue growth factor, hepatocyte growth factor and teratocarcinoma-derived growth factor 1 on the development of the bovine preimplantation embryo.
Kannampuzha-Francis J, Tribulo P, Hansen PJ
Reproduction, fertility, and development29(7)1329-1339(2017 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells. Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics.
Chowdhury I, Chaqour B
European journal of biochemistry271(22)4436-50(2004 Nov)

Differential regulation of biglycan and decorin synthesis by connective tissue growth factor in cultured vascular endothelial cells.
Kaji T, Yamamoto C, Oh-i M, Nishida T, Takigawa M
Biochemical and biophysical research communications322(1)22-8(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Actin cytoskeleton-dependent regulation of CTGF transcription and mRNA stability
Title: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells. Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics.

The results indicate that CTGF suppresses the synthesis of biglycan but newly induced that of decorin in the cells when the cell density is low.
Title: Differential regulation of biglycan and decorin synthesis by connective tissue growth factor in cultured vascular endothelial cells.

The following CCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41803	NM_174030.2 Latest version!	Bos taurus cellular communication network factor 2 (CCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCN2 ( NM_174030.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776455.1 Bos taurus cellular communication network factor 2 (CCN2), mRNA.

Price & Availability↑

CloneID	OBa41803
Clone ID Related Accession (Same CDS sequence)	NM_174030.2
Accession Version	NM_174030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	CCN family member 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF000137.1. On Jun 3, 2003 this sequence version replaced NM_174030.1. ##Evidence-Data-START## Transcript exon combination :: AF000137.1, DV890626.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGCCA CCGGCCTGGG CCCGGTCCGC TGCGCCTTCG TGCTCCTGCT CGCCCTCTGC 
AGCCGGCCCG CCTCCAGCCA GGACTGCTGC AGCGCCCCGT GCCAGTGCCC TGCCGGCCCG 
GCGCCGCGCT GCCCCGCCGG CGTCAGCTTG GTGCTGGACG GCTGCGGCTG CTGCGTGTGC 
GCCAAGCAGC TGAGCGAGTT GTGTACCGAG CGCGACCCCT GCGATCCGCA CAAGGGCCTC 
TTCTGCGACT TCGGCTCCCC AACCAACCGC AAGATCGGCG TGTGCACCGC TAAAGATGGT 
GCCCCCTACA TTTTCGGAGG AACTGTGTAC CAGAGCGGAG AGTCTTTCCA GAGCAGCTGC 
AAATACCAGT GCACGTGTCT GGACGGGTCG GTGGGCTGCG TGCCCCTATG CAGCGTGGAC 
GTCCGCCTGC CCAGCCCCGA CTGCCCCTTC CCACGGAGGG TCAAACTGCC CGGGAAATGC 
TGCGAGGAAT GGGTGTCCCG TGATGAGAAG GAGCACACCG TGGTCGGCCC TGCGCTGGCA 
GCTTACCGGC TGGAAGACAC GTTTGGCCCA GACCCAACCA TGATCCGAGC CAACTGCCAG 
GTCCAGACCA CAGAGTGGAG TGCCTATTCC AAGACCTGCG GAATGGGCAT CTCCACCCGG 
GTTACCAATG ACAACGCATT CTGCAGGCTG GAGAAGCAGA GCCGCCTCTG CATGGTCAGG 
CCTTGCGAAG CTGACCTGGA GGAGAACATT AAGAAAGGCA AAAAGTGCAT CCGGACTCCC 
AAAATCTCCA AGCCTATCAA GTTTCAGCTT TCTGGCTGCA CCAGCATGAA GACATACCGA 
GCTAAATTCT TCGGAGTGTG CACAGACGGG CGGTGCTGCA CCCCCCACAG AACCACCACC 
CTTCCCGTGG AGTTCAAGTG TCCTGATGGG GAGGTCATGA AGAAGAGCAT GATGTTCATC 
AAGACCTGTG CCTGCCATTA CAACTGCCCC GGAGACAATG ACATCTTCGA GTCACTGTAC 
TACAGGAAGA TGTATGGAGA CATGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776455.1
CDS	206..1255
Translation	MSATGLGPVRCAFVLLLALCSRPASSQDCCSAPCQCPAGPAPRCPAGVSLVLDGCGCCVCAKQLSELCTERDPCDPHKGLFCDFGSPTNRKIGVCTAKDGAPYIFGGTVYQSGESFQSSCKYQCTCLDGSVGCVPLCSVDVRLPSPDCPFPRRVKLPGKCCEEWVSRDEKEHTVVGPALAAYRLEDTFGPDPTMIRANCQVQTTEWSAYSKTCGMGISTRVTNDNAFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKISKPIKFQLSGCTSMKTYRAKFFGVCTDGRCCTPHRTTTLPVEFKCPDGEVMKKSMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

Target ORF information:

RefSeq Version	NM_174030.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cellular communication network factor 2 (CCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174030.2

ORF Insert Sequence:

ATGTCAGCCA CCGGCCTGGG CCCGGTCCGC TGCGCCTTCG TGCTCCTGCT CGCCCTCTGC 
AGCCGGCCCG CCTCCAGCCA GGACTGCTGC AGCGCCCCGT GCCAGTGCCC TGCCGGCCCG 
GCGCCGCGCT GCCCCGCCGG CGTCAGCTTG GTGCTGGACG GCTGCGGCTG CTGCGTGTGC 
GCCAAGCAGC TGAGCGAGTT GTGTACCGAG CGCGACCCCT GCGATCCGCA CAAGGGCCTC 
TTCTGCGACT TCGGCTCCCC AACCAACCGC AAGATCGGCG TGTGCACCGC TAAAGATGGT 
GCCCCCTACA TTTTCGGAGG AACTGTGTAC CAGAGCGGAG AGTCTTTCCA GAGCAGCTGC 
AAATACCAGT GCACGTGTCT GGACGGGTCG GTGGGCTGCG TGCCCCTATG CAGCGTGGAC 
GTCCGCCTGC CCAGCCCCGA CTGCCCCTTC CCACGGAGGG TCAAACTGCC CGGGAAATGC 
TGCGAGGAAT GGGTGTCCCG TGATGAGAAG GAGCACACCG TGGTCGGCCC TGCGCTGGCA 
GCTTACCGGC TGGAAGACAC GTTTGGCCCA GACCCAACCA TGATCCGAGC CAACTGCCAG 
GTCCAGACCA CAGAGTGGAG TGCCTATTCC AAGACCTGCG GAATGGGCAT CTCCACCCGG 
GTTACCAATG ACAACGCATT CTGCAGGCTG GAGAAGCAGA GCCGCCTCTG CATGGTCAGG 
CCTTGCGAAG CTGACCTGGA GGAGAACATT AAGAAAGGCA AAAAGTGCAT CCGGACTCCC 
AAAATCTCCA AGCCTATCAA GTTTCAGCTT TCTGGCTGCA CCAGCATGAA GACATACCGA 
GCTAAATTCT TCGGAGTGTG CACAGACGGG CGGTGCTGCA CCCCCCACAG AACCACCACC 
CTTCCCGTGG AGTTCAAGTG TCCTGATGGG GAGGTCATGA AGAAGAGCAT GATGTTCATC 
AAGACCTGTG CCTGCCATTA CAACTGCCCC GGAGACAATG ACATCTTCGA GTCACTGTAC 
TACAGGAAGA TGTATGGAGA CATGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Actions of activin A, connective tissue growth factor, hepatocyte growth factor and teratocarcinoma-derived growth factor 1 on the development of the bovine preimplantation embryo.
Reproduction, fertility, and development29(7)1329-1339(2017 Jul)
Kannampuzha-Francis J,Tribulo P,Hansen PJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells. Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics.
European journal of biochemistry271(22)4436-50(2004 Nov)
Chowdhury I,Chaqour B

Differential regulation of biglycan and decorin synthesis by connective tissue growth factor in cultured vascular endothelial cells.
Biochemical and biophysical research communications322(1)22-8(2004 Sep)
Kaji T,Yamamoto C,Oh-i M,Nishida T,Takigawa M

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CCN2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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