CD14 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD14
Entrez Gene ID	281048
Full Name	CD14 molecule
General protein information	Preferred Names CD14 molecule Names monocyte differentiation antigen CD14 CD14 antigen myeloid cell-specific leucine-rich glycoprotein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005209429.4	XP_005209486.1	monocyte differentiation antigen CD14 isoform X1
NM_174008.1	NP_776433.1	monocyte differentiation antigen CD14 precursor
XM_005209429.3	XP_005209486.1	monocyte differentiation antigen CD14 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CD14	XP_848746.2
	CD14	NP_001123905.1
Pan troglodytes (chimpanzee)	CD14	NP_001123938.1
Homo sapiens (human)	CD14	NP_000582.1
Bos taurus (cattle)	CD14	NP_776433.1
Mus musculus (house mouse)	Cd14	NP_033971.1
Rattus norvegicus (Norway rat)	Cd14	NP_068512.1

Gene Ontology
Bibliography

Related articles in PubMed

SNPs of CD14 change the mastitis morbidity of Chinese Holstein.
Li J, Wang Q, Chen F, Wang H, Chen J, Wang Z, Huo J, Cai Y
Molecular medicine reports16(6)9102-9110(2017 Dec)

Changes in endometrial transcription of TLR2, TLR4, and CD14 during the first-week postpartum in dairy cows with retained placenta.
Martins TM, Muniz CS, Andrade VB, Paix?o TA, Santos RL, Borges ?M
Theriogenology85(7)1282-8(2016 Apr)

Time course of defense mechanisms in bovine endometrium in response to lipopolysaccharide.
Swangchan-Uthai T, Lavender CR, Cheng Z, Fouladi-Nashta AA, Wathes DC
Biology of reproduction87(6)135(2012 Jun)

Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland.
Sladek Z, Rysanek D
Research in veterinary science90(2)226-34(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transcription levels of TLR2, TLR4, and CD14 in Holstein cows with retained placenta significantly decreased between the first and the seventh day postpartum.
Title: Changes in endometrial transcription of TLR2, TLR4, and CD14 during the first-week postpartum in dairy cows with retained placenta.

Data from an in vitro co-culture model suggest that an early response of endometrium in uterine infection is up-regulation of expression of CD14 and TLR4 (toll-like receptor 4).
Title: Time course of defense mechanisms in bovine endometrium in response to lipopolysaccharide.

This study study confirmed that expression of CD14 in blood polymorpnuclear cells (PMN), resident PMN and inflammatory PMN from heifer mammary glands was accompanied by apoptosis and necrosis.
Title: Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland.

SNP and association analyses have provided baseline information that may be used at defining the role of CD14 in mediating bacterial infections
Title: Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils.

First report of a functionally active animal receptor protein made in plants and the prophylactic use of a plant-derived protein to reduce the severity of bacterial infections in animals.
Title: Bovine CD14 receptor produced in plants reduces severity of intramammary bacterial infection.

The following CD14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41822	NM_174008.1 Latest version!	Bos taurus CD14 molecule (CD14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa21527	XM_005209429.4 Latest version!	Bos taurus CD14 molecule (CD14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OBa21527	XM_005209429.3	Bos taurus CD14 molecule (CD14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD14 ( NM_174008.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776433.1 Bos taurus CD14 molecule (CD14), mRNA.

Price & Availability↑

CloneID	OBa41822
Clone ID Related Accession (Same CDS sequence)	NM_174008.1
Accession Version	NM_174008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	monocyte differentiation antigen CD14 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF141313.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF141313.1, EV624527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGTGTGCG TGCCCTACCT GCTGCTGCTG CTGCTGCCGT CACTGCTGCG TGTGTCTGCG 
GACACAACAG AACCCTGCGA GCTGGACGAC GACGATTTCC GTTGTGTCTG CAACTTCACG 
GATCCGAAGC CTGACTGGTC TAGCGCCGTT CAGTGTATGG TTGCCGTCGA GGTGGAGATC 
AGTGCCGGCG GCCGCAGCCT GGAACAGTTT CTCAAGGGAG CCGACACCAA CCCGAAGCAG 
TATGCTGACA CAATCAAGGC TCTGCGCGTT CGGCGACTCA AGCTGGGCGC TGCACAGGTT 
CCTGCTCAGC TTCTGGTCGC CGTTCTGCGC GCGCTCGGGT ACTCTCGTCT CAAGGAACTG 
ACGCTTGAGG ACCTGGAGGT AACCGGCCCA ACGCCCCCGA CGCCTCTGGA AGCCGCTGGG 
CCTGCGCTCA CCACCCTCAG TCTCCGTAAC GTATCGTGGA CAACAGGAGG TGCCTGGCTC 
GGCGAACTGC AGCAGTGGCT CAAGCCTGGG CTCAGGGTGC TGAACATTGC CCAAGCACAC 
TCGCTTGCCT TTCCGTGCGC AGGGCTCTCC ACCTTCGAGG CGCTCACCAC CCTAGACCTG 
TCTGACAATC CCAGTCTCGG CGACACGGGG CTGATGGCAG CTCTCTGTCC GAACAAGTTC 
CCGGCCCTCC AATATCTAGC GCTACGCAAC GCGGGGATGG AGACGCCGAG CGGCGTGTGC 
GCGGCGCTGG CGGCAGCGAG GGTGCAGCCC CAAAGCCTGG ACCTCAGCCA CAACTCGCTG 
CGCGTCACCG CCCCGGGTGC TACCCGATGT GTCTGGCCCA GTGCACTAAG GTCTCTCAAT 
TTGTCGTTCG CTGGGCTGGA GCAAGTGCCT AAGGGACTGC CCCCTAAGCT CAGCGTGCTT 
GATCTCAGCT GCAACAAGCT AAGCAGGGAG CCGCGGCGAG ACGAGCTGCC CGAGGTAAAT 
GACCTGACTC TGGACGGAAA TCCCTTTCTG GACCCTGGAG CCCTCCAGCA CCAAAATGAC 
CCGATGATCT CCGGCGTGGT CCCAGCCTGT GCGCGTTCTG CCTTGACCAT GGGGGTGTCA 
GGAGCCCTGG CGCTGCTTCA AGGAGCCCGA GGCTTCGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776433.1
CDS	83..1204
Translation	MVCVPYLLLLLLPSLLRVSADTTEPCELDDDDFRCVCNFTDPKPDWSSAVQCMVAVEVEISAGGRSLEQFLKGADTNPKQYADTIKALRVRRLKLGAAQVPAQLLVAVLRALGYSRLKELTLEDLEVTGPTPPTPLEAAGPALTTLSLRNVSWTTGGAWLGELQQWLKPGLRVLNIAQAHSLAFPCAGLSTFEALTTLDLSDNPSLGDTGLMAALCPNKFPALQYLALRNAGMETPSGVCAALAAARVQPQSLDLSHNSLRVTAPGATRCVWPSALRSLNLSFAGLEQVPKGLPPKLSVLDLSCNKLSREPRRDELPEVNDLTLDGNPFLDPGALQHQNDPMISGVVPACARSALTMGVSGALALLQGARGFA

Target ORF information:

RefSeq Version	NM_174008.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD14 molecule (CD14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174008.1

ORF Insert Sequence:

ATGGTGTGCG TGCCCTACCT GCTGCTGCTG CTGCTGCCGT CACTGCTGCG TGTGTCTGCG 
GACACAACAG AACCCTGCGA GCTGGACGAC GACGATTTCC GTTGTGTCTG CAACTTCACG 
GATCCGAAGC CTGACTGGTC TAGCGCCGTT CAGTGTATGG TTGCCGTCGA GGTGGAGATC 
AGTGCCGGCG GCCGCAGCCT GGAACAGTTT CTCAAGGGAG CCGACACCAA CCCGAAGCAG 
TATGCTGACA CAATCAAGGC TCTGCGCGTT CGGCGACTCA AGCTGGGCGC TGCACAGGTT 
CCTGCTCAGC TTCTGGTCGC CGTTCTGCGC GCGCTCGGGT ACTCTCGTCT CAAGGAACTG 
ACGCTTGAGG ACCTGGAGGT AACCGGCCCA ACGCCCCCGA CGCCTCTGGA AGCCGCTGGG 
CCTGCGCTCA CCACCCTCAG TCTCCGTAAC GTATCGTGGA CAACAGGAGG TGCCTGGCTC 
GGCGAACTGC AGCAGTGGCT CAAGCCTGGG CTCAGGGTGC TGAACATTGC CCAAGCACAC 
TCGCTTGCCT TTCCGTGCGC AGGGCTCTCC ACCTTCGAGG CGCTCACCAC CCTAGACCTG 
TCTGACAATC CCAGTCTCGG CGACACGGGG CTGATGGCAG CTCTCTGTCC GAACAAGTTC 
CCGGCCCTCC AATATCTAGC GCTACGCAAC GCGGGGATGG AGACGCCGAG CGGCGTGTGC 
GCGGCGCTGG CGGCAGCGAG GGTGCAGCCC CAAAGCCTGG ACCTCAGCCA CAACTCGCTG 
CGCGTCACCG CCCCGGGTGC TACCCGATGT GTCTGGCCCA GTGCACTAAG GTCTCTCAAT 
TTGTCGTTCG CTGGGCTGGA GCAAGTGCCT AAGGGACTGC CCCCTAAGCT CAGCGTGCTT 
GATCTCAGCT GCAACAAGCT AAGCAGGGAG CCGCGGCGAG ACGAGCTGCC CGAGGTAAAT 
GACCTGACTC TGGACGGAAA TCCCTTTCTG GACCCTGGAG CCCTCCAGCA CCAAAATGAC 
CCGATGATCT CCGGCGTGGT CCCAGCCTGT GCGCGTTCTG CCTTGACCAT GGGGGTGTCA 
GGAGCCCTGG CGCTGCTTCA AGGAGCCCGA GGCTTCGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD14 ( XM_005209429.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209486.1 Bos taurus CD14 molecule (CD14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21527
Clone ID Related Accession (Same CDS sequence)	XM_005209429.4 , XM_005209429.3
Accession Version	XM_005209429.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	monocyte differentiation antigen CD14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209429.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAG TGTGCGTGCC CTACCTGCTG CTGCTGCTGC TGCCGTCACT GCTGCGTGTG 
TCTGCGGACA CAACAGAACC CTGCGAGCTG GACGACGACG ATTTCCGTTG TGTCTGCAAC 
TTCACGGATC CGAAGCCTGA CTGGTCTAGC GCCGTTCAGT GTATGGTTGC CGTCGAGGTG 
GAGATCAGTG CCGGCGGCCG CAGCCTGGAA CAGTTTCTCA AGGGAGCCGA CACCAACCCG 
AAGCAGTATG CTGACACAAT CAAGGCTCTG CGCGTTCGGC GACTCAAGCT GGGCGCTGCA 
CAGGTTCCTG CTCAGCTTCT GGTCGCCGTT CTGCGCGCGC TCGGGTACTC TCGTCTCAAG 
GAACTGACGC TTGAGGACCT GGAGGTAACC GGCCCAACGC CCCCGACGCC TCTGGAAGCC 
GCTGGGCCTG CGCTCACCAC CCTCAGTCTC CGTAACGTAT CGTGGACAAC AGGAGGTGCC 
TGGCTCGGCG AACTGCAGCA GTGGCTCAAG CCTGGGCTCA GGGTGCTGAA CATTGCCCAA 
GCACACTCGC TTGCCTTTCC GTGCGCAGGG CTCTCCACCT TCGAGGCGCT CACCACCCTA 
GACCTGTCTG ACAATCCCAG TCTCGGCGAC AGCGGGCTGA TGGCAGCTCT CTGTCCGAAC 
AAGTTCCCGG CCCTCCAATA TCTAGCGCTA CGCAACGCGG GGATGGAGAC GCCGAGCGGC 
GTGTGCGCGG CGCTGGCGGC AGCGAGGGTG CAGCCCCAAA GCCTGGACCT CAGCCACAAC 
TCGCTGCGCG TCACCGCCCC GGGTGCTACC CGATGTGTCT GGCCCAGTGC ACTAAGGTCT 
CTCAATTTGT CGTTCGCTGG GCTGGAGCAA GTGCCTAAGG GACTGCCCCC TAAGCTCAGC 
GTGCTTGATC TCAGCTGCAA CAAGCTAAGC AGGGAGCCAC GGCGAGACGA GCTGCCCGAG 
GTAAATGACC TGACTCTGGA CGGAAATCCC TTTCTGGACC CTGGAGCCCT CCAGCACCAA 
AATGACCCGA TGATCTCCGG CGTGGTCCCA GCCTGTGCGC GTTCTGCCTT GACCATGGGG 
GTGTCAGGAG CCCTGGCGCT GCTTCAAGGA GCCCGAGGCT TCGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209486.1
CDS	205..1332
Translation	MVRVCVPYLLLLLLPSLLRVSADTTEPCELDDDDFRCVCNFTDPKPDWSSAVQCMVAVEVEISAGGRSLEQFLKGADTNPKQYADTIKALRVRRLKLGAAQVPAQLLVAVLRALGYSRLKELTLEDLEVTGPTPPTPLEAAGPALTTLSLRNVSWTTGGAWLGELQQWLKPGLRVLNIAQAHSLAFPCAGLSTFEALTTLDLSDNPSLGDSGLMAALCPNKFPALQYLALRNAGMETPSGVCAALAAARVQPQSLDLSHNSLRVTAPGATRCVWPSALRSLNLSFAGLEQVPKGLPPKLSVLDLSCNKLSREPRRDELPEVNDLTLDGNPFLDPGALQHQNDPMISGVVPACARSALTMGVSGALALLQGARGFA

Target ORF information:

RefSeq Version	XM_005209429.4
Organism	Bos taurus(cattle)
Definition	Bos taurus CD14 molecule (CD14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209429.4

ORF Insert Sequence:

ATGGTGAGAG TGTGCGTGCC CTACCTGCTG CTGCTGCTGC TGCCGTCACT GCTGCGTGTG 
TCTGCGGACA CAACAGAACC CTGCGAGCTG GACGACGACG ATTTCCGTTG TGTCTGCAAC 
TTCACGGATC CGAAGCCTGA CTGGTCTAGC GCCGTTCAGT GTATGGTTGC CGTCGAGGTG 
GAGATCAGTG CCGGCGGCCG CAGCCTGGAA CAGTTTCTCA AGGGAGCCGA CACCAACCCG 
AAGCAGTATG CTGACACAAT CAAGGCTCTG CGCGTTCGGC GACTCAAGCT GGGCGCTGCA 
CAGGTTCCTG CTCAGCTTCT GGTCGCCGTT CTGCGCGCGC TCGGGTACTC TCGTCTCAAG 
GAACTGACGC TTGAGGACCT GGAGGTAACC GGCCCAACGC CCCCGACGCC TCTGGAAGCC 
GCTGGGCCTG CGCTCACCAC CCTCAGTCTC CGTAACGTAT CGTGGACAAC AGGAGGTGCC 
TGGCTCGGCG AACTGCAGCA GTGGCTCAAG CCTGGGCTCA GGGTGCTGAA CATTGCCCAA 
GCACACTCGC TTGCCTTTCC GTGCGCAGGG CTCTCCACCT TCGAGGCGCT CACCACCCTA 
GACCTGTCTG ACAATCCCAG TCTCGGCGAC AGCGGGCTGA TGGCAGCTCT CTGTCCGAAC 
AAGTTCCCGG CCCTCCAATA TCTAGCGCTA CGCAACGCGG GGATGGAGAC GCCGAGCGGC 
GTGTGCGCGG CGCTGGCGGC AGCGAGGGTG CAGCCCCAAA GCCTGGACCT CAGCCACAAC 
TCGCTGCGCG TCACCGCCCC GGGTGCTACC CGATGTGTCT GGCCCAGTGC ACTAAGGTCT 
CTCAATTTGT CGTTCGCTGG GCTGGAGCAA GTGCCTAAGG GACTGCCCCC TAAGCTCAGC 
GTGCTTGATC TCAGCTGCAA CAAGCTAAGC AGGGAGCCAC GGCGAGACGA GCTGCCCGAG 
GTAAATGACC TGACTCTGGA CGGAAATCCC TTTCTGGACC CTGGAGCCCT CCAGCACCAA 
AATGACCCGA TGATCTCCGG CGTGGTCCCA GCCTGTGCGC GTTCTGCCTT GACCATGGGG 
GTGTCAGGAG CCCTGGCGCT GCTTCAAGGA GCCCGAGGCT TCGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD14 ( XM_005209429.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209486.1 Bos taurus CD14 molecule (CD14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21527
Clone ID Related Accession (Same CDS sequence)	XM_005209429.4 , XM_005209429.3
Accession Version	XM_005209429.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	monocyte differentiation antigen CD14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104035.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAG TGTGCGTGCC CTACCTGCTG CTGCTGCTGC TGCCGTCACT GCTGCGTGTG 
TCTGCGGACA CAACAGAACC CTGCGAGCTG GACGACGACG ATTTCCGTTG TGTCTGCAAC 
TTCACGGATC CGAAGCCTGA CTGGTCTAGC GCCGTTCAGT GTATGGTTGC CGTCGAGGTG 
GAGATCAGTG CCGGCGGCCG CAGCCTGGAA CAGTTTCTCA AGGGAGCCGA CACCAACCCG 
AAGCAGTATG CTGACACAAT CAAGGCTCTG CGCGTTCGGC GACTCAAGCT GGGCGCTGCA 
CAGGTTCCTG CTCAGCTTCT GGTCGCCGTT CTGCGCGCGC TCGGGTACTC TCGTCTCAAG 
GAACTGACGC TTGAGGACCT GGAGGTAACC GGCCCAACGC CCCCGACGCC TCTGGAAGCC 
GCTGGGCCTG CGCTCACCAC CCTCAGTCTC CGTAACGTAT CGTGGACAAC AGGAGGTGCC 
TGGCTCGGCG AACTGCAGCA GTGGCTCAAG CCTGGGCTCA GGGTGCTGAA CATTGCCCAA 
GCACACTCGC TTGCCTTTCC GTGCGCAGGG CTCTCCACCT TCGAGGCGCT CACCACCCTA 
GACCTGTCTG ACAATCCCAG TCTCGGCGAC AGCGGGCTGA TGGCAGCTCT CTGTCCGAAC 
AAGTTCCCGG CCCTCCAATA TCTAGCGCTA CGCAACGCGG GGATGGAGAC GCCGAGCGGC 
GTGTGCGCGG CGCTGGCGGC AGCGAGGGTG CAGCCCCAAA GCCTGGACCT CAGCCACAAC 
TCGCTGCGCG TCACCGCCCC GGGTGCTACC CGATGTGTCT GGCCCAGTGC ACTAAGGTCT 
CTCAATTTGT CGTTCGCTGG GCTGGAGCAA GTGCCTAAGG GACTGCCCCC TAAGCTCAGC 
GTGCTTGATC TCAGCTGCAA CAAGCTAAGC AGGGAGCCAC GGCGAGACGA GCTGCCCGAG 
GTAAATGACC TGACTCTGGA CGGAAATCCC TTTCTGGACC CTGGAGCCCT CCAGCACCAA 
AATGACCCGA TGATCTCCGG CGTGGTCCCA GCCTGTGCGC GTTCTGCCTT GACCATGGGG 
GTGTCAGGAG CCCTGGCGCT GCTTCAAGGA GCCCGAGGCT TCGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209486.1
CDS	212..1339
Translation	MVRVCVPYLLLLLLPSLLRVSADTTEPCELDDDDFRCVCNFTDPKPDWSSAVQCMVAVEVEISAGGRSLEQFLKGADTNPKQYADTIKALRVRRLKLGAAQVPAQLLVAVLRALGYSRLKELTLEDLEVTGPTPPTPLEAAGPALTTLSLRNVSWTTGGAWLGELQQWLKPGLRVLNIAQAHSLAFPCAGLSTFEALTTLDLSDNPSLGDSGLMAALCPNKFPALQYLALRNAGMETPSGVCAALAAARVQPQSLDLSHNSLRVTAPGATRCVWPSALRSLNLSFAGLEQVPKGLPPKLSVLDLSCNKLSREPRRDELPEVNDLTLDGNPFLDPGALQHQNDPMISGVVPACARSALTMGVSGALALLQGARGFA

Target ORF information:

RefSeq Version	XM_005209429.3
Organism	Bos taurus(cattle)
Definition	Bos taurus CD14 molecule (CD14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209429.3

ORF Insert Sequence:

ATGGTGAGAG TGTGCGTGCC CTACCTGCTG CTGCTGCTGC TGCCGTCACT GCTGCGTGTG 
TCTGCGGACA CAACAGAACC CTGCGAGCTG GACGACGACG ATTTCCGTTG TGTCTGCAAC 
TTCACGGATC CGAAGCCTGA CTGGTCTAGC GCCGTTCAGT GTATGGTTGC CGTCGAGGTG 
GAGATCAGTG CCGGCGGCCG CAGCCTGGAA CAGTTTCTCA AGGGAGCCGA CACCAACCCG 
AAGCAGTATG CTGACACAAT CAAGGCTCTG CGCGTTCGGC GACTCAAGCT GGGCGCTGCA 
CAGGTTCCTG CTCAGCTTCT GGTCGCCGTT CTGCGCGCGC TCGGGTACTC TCGTCTCAAG 
GAACTGACGC TTGAGGACCT GGAGGTAACC GGCCCAACGC CCCCGACGCC TCTGGAAGCC 
GCTGGGCCTG CGCTCACCAC CCTCAGTCTC CGTAACGTAT CGTGGACAAC AGGAGGTGCC 
TGGCTCGGCG AACTGCAGCA GTGGCTCAAG CCTGGGCTCA GGGTGCTGAA CATTGCCCAA 
GCACACTCGC TTGCCTTTCC GTGCGCAGGG CTCTCCACCT TCGAGGCGCT CACCACCCTA 
GACCTGTCTG ACAATCCCAG TCTCGGCGAC AGCGGGCTGA TGGCAGCTCT CTGTCCGAAC 
AAGTTCCCGG CCCTCCAATA TCTAGCGCTA CGCAACGCGG GGATGGAGAC GCCGAGCGGC 
GTGTGCGCGG CGCTGGCGGC AGCGAGGGTG CAGCCCCAAA GCCTGGACCT CAGCCACAAC 
TCGCTGCGCG TCACCGCCCC GGGTGCTACC CGATGTGTCT GGCCCAGTGC ACTAAGGTCT 
CTCAATTTGT CGTTCGCTGG GCTGGAGCAA GTGCCTAAGG GACTGCCCCC TAAGCTCAGC 
GTGCTTGATC TCAGCTGCAA CAAGCTAAGC AGGGAGCCAC GGCGAGACGA GCTGCCCGAG 
GTAAATGACC TGACTCTGGA CGGAAATCCC TTTCTGGACC CTGGAGCCCT CCAGCACCAA 
AATGACCCGA TGATCTCCGG CGTGGTCCCA GCCTGTGCGC GTTCTGCCTT GACCATGGGG 
GTGTCAGGAG CCCTGGCGCT GCTTCAAGGA GCCCGAGGCT TCGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SNPs of CD14 change the mastitis morbidity of Chinese Holstein.
Molecular medicine reports16(6)9102-9110(2017 Dec)
Li J,Wang Q,Chen F,Wang H,Chen J,Wang Z,Huo J,Cai Y

Changes in endometrial transcription of TLR2, TLR4, and CD14 during the first-week postpartum in dairy cows with retained placenta.
Theriogenology85(7)1282-8(2016 Apr)
Martins TM,Muniz CS,Andrade VB,Paix?o TA,Santos RL,Borges ?M

Time course of defense mechanisms in bovine endometrium in response to lipopolysaccharide.
Biology of reproduction87(6)135(2012 Jun)
Swangchan-Uthai T,Lavender CR,Cheng Z,Fouladi-Nashta AA,Wathes DC

Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland.
Research in veterinary science90(2)226-34(2011 Apr)
Sladek Z,Rysanek D

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CD14 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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