Cd14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd14
Entrez Gene ID	60350
Full Name	CD14 molecule
General protein information	Preferred Names CD14 molecule Names monocyte differentiation antigen CD14 CD14 antigen myeloid cell-specific leucine-rich glycoprotein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p11
Summary	component of the lipopolysaccharide receptor complex; mediates LPS-induced neuroinflammation and inflammatory response [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006254603.3	XP_006254665.1	monocyte differentiation antigen CD14 isoform X1
NM_021744.1	NP_068512.1	monocyte differentiation antigen CD14 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CD14	XP_848746.2
	CD14	NP_001123905.1
Pan troglodytes (chimpanzee)	CD14	NP_001123938.1
Homo sapiens (human)	CD14	NP_000582.1
Bos taurus (cattle)	CD14	NP_776433.1
Mus musculus (house mouse)	Cd14	NP_033971.1
Rattus norvegicus (Norway rat)	Cd14	NP_068512.1

Gene Ontology
Bibliography

Related articles in PubMed

Soluble CD14 inhibits contractile function and insulin action in primary adult rat cardiomyocytes.
Overhagen S, Blumensatt M, Fahlbusch P, Herzfeld de Wiza D, M?ller H, Maxhera B, Akhyari P, Ouwens DM
Biochimica et biophysica acta1863(2)365-374(2017 Feb)

The kinetic expression of lipopolysaccharide-binding protein and CD14 gene in obstructive jaundice.
Tsai CH, Yeh CH, Sheen-Chen SM, Huang CY, Liu YW, Huang CC, Shen SC, Tang RP
Journal of investigative surgery : the official journal of the Academy of Surgical Research28(1)18-23(2015 Feb)

The attenuated inflammation of MPL is due to the lack of CD14-dependent tight dimerization of the TLR4/MD2 complex at the plasma membrane.
Tanimura N, Saitoh S, Ohto U, Akashi-Takamura S, Fujimoto Y, Fukase K, Shimizu T, Miyake K
International immunology26(6)307-14(2014 Jun)

Effect of biliary drainage on inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats.
Wang ZK, Xiao JG, Huang XF, Gong YC, Li W
World journal of gastroenterology19(15)2319-30(2013 Apr)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CD 14 mRNA expression increased significantly after five days of bile duct ligation in liver, lung, spleen, and ileum.
Title: The kinetic expression of lipopolysaccharide-binding protein and CD14 gene in obstructive jaundice.

biliary drainage affects expression of inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats
Title: Effect of biliary drainage on inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats.

Hepatic ischemia-reperfusion injury up-regulated CD14 and TLR4 gene expression in Kupffer cells, and subsequently activated NF-kappabeta to produce cytokines.
Title: The role of CD14 and Toll-like receptor 4 of Kupffer cells in hepatic ischemia-reperfusion injury in rats.

Show that pineal glands possess both CD14 and toll-like receptor 4 (TLR4), membrane proteins that bind LPS and trigger the NFKB pathway.
Title: TLR4 and CD14 receptors expressed in rat pineal gland trigger NFKB pathway.

Burn injury-induced mitochondrial damage in cardiomyocytes was prevented in CD14 knockout mice. Burn injury produces CD14-dependent mitochondrial damage via oxidative stress in myocardium.
Title: Burn serum causes a CD14-dependent mitochondrial damage in primary cardiomyocytes.

The following Cd14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13556	NM_021744.1 Latest version!	Rattus norvegicus CD14 molecule (Cd14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa38318	XM_006254603.3 Latest version!	Rattus norvegicus CD14 molecule (Cd14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd14 ( NM_021744.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068512.1 Rattus norvegicus CD14 molecule (Cd14), mRNA.

Price & Availability↑

CloneID	ORa13556
Clone ID Related Accession (Same CDS sequence)	NM_021744.1
Accession Version	NM_021744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	monocyte differentiation antigen CD14 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF087943.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF087943.1, FQ219480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTTA TGCTCGGCTT GTTGCTGTTG CCTTTGACAC TGGTGCATGC CTCTCCCGCC 
ACACCAGAAC CCTGTGAGCT GGACCAGGAC GAGGAAAGTG TCCGCTGCTA CTGCAACTTC 
TCAGATCCGC AGCCCAATTG GTCCAGCGCT TTTCTATGCG CGGGGGCGGA AGACGTGGAG 
TTCTACGGCG GCGGTCGCAG CCTGGAGTAC CTTCTAAAGC GTGTGGACAC GGAAGCAAAT 
CTGGGGCAGT ACACTGATAT TATCAGGTCT CTGCCCTTGA AGAGGCTTAC TGTGCGGTCC 
GCGCGGGTTC CTACTCAGAT TCTATTCGGA ACCCTGCGTG TGCTCGGATA TTCTGGCCTC 
CGGGAACTGA CTCTTGAAAA CCTCGAGGTA ACCGGCACCG CGCTTTCGCC GCTTCTGGAT 
GCCACCGGAC CCGATCTCAA CACCTTGAGC CTCCGCAACG TGTCGTGGGC AACAACGGAT 
ACCTGGCTCG CAGAACTGCA GCAGTGGCTA AAGCCTGGAC TCAAGGTACT AAGTATTGCC 
CAAGCACACT CACTCAACTT TTCTTGCAAA CAGGTCGGCG TCTTCCCTGC CCTTGCCACC 
TTAGATCTGT CTGACAACCC TGAGTTGGGC GAGAAAGGAC TGATCTCGGC CCTCTGTCCG 
CACAAATTCC CGACCCTCCA AGTTTTAGCG CTGCGCAACG CGGGGATGGA GACGACCAGC 
GGCGTGTGCT CTGCGCTGGC CGCAGCAAGA GTGCCACTGC AGGCACTGGA TCTCAGTCAC 
AATTCACTGC GGGATACTGC TGGCACTCCG AGCTGTGACT GGCCCAGTCA GCTAAACTCG 
CTCAATCTAT CTTTCACTGG GCTGGAGCAC GTACCTAAAG GGCTGCCAGC CAAGCTTAGC 
GTGCTTGATC TCAGTTACAA CAGGCTGGAT AGGAAACCTA GGCCAGAGGA GCTGCCTGAA 
GTGGGGAGCC TGTCACTTAC AGGAAATCCC TTTTTGCACT CTGAATCCCA GTCGGAGGCG 
TATAACTCTG GCGTAGTCAT AGCCACAGCT CTTTCACCCG GATCAGCGGG CTTGTCAGGA 
ACTTTGGCTT TGCTCCTAGG ACATCGCCTC TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068512.1
CDS	66..1184
Translation	MKLMLGLLLLPLTLVHASPATPEPCELDQDEESVRCYCNFSDPQPNWSSAFLCAGAEDVEFYGGGRSLEYLLKRVDTEANLGQYTDIIRSLPLKRLTVRSARVPTQILFGTLRVLGYSGLRELTLENLEVTGTALSPLLDATGPDLNTLSLRNVSWATTDTWLAELQQWLKPGLKVLSIAQAHSLNFSCKQVGVFPALATLDLSDNPELGEKGLISALCPHKFPTLQVLALRNAGMETTSGVCSALAAARVPLQALDLSHNSLRDTAGTPSCDWPSQLNSLNLSFTGLEHVPKGLPAKLSVLDLSYNRLDRKPRPEELPEVGSLSLTGNPFLHSESQSEAYNSGVVIATALSPGSAGLSGTLALLLGHRLFV

Target ORF information:

RefSeq Version	NM_021744.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD14 molecule (Cd14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021744.1

ORF Insert Sequence:

ATGAAGCTTA TGCTCGGCTT GTTGCTGTTG CCTTTGACAC TGGTGCATGC CTCTCCCGCC 
ACACCAGAAC CCTGTGAGCT GGACCAGGAC GAGGAAAGTG TCCGCTGCTA CTGCAACTTC 
TCAGATCCGC AGCCCAATTG GTCCAGCGCT TTTCTATGCG CGGGGGCGGA AGACGTGGAG 
TTCTACGGCG GCGGTCGCAG CCTGGAGTAC CTTCTAAAGC GTGTGGACAC GGAAGCAAAT 
CTGGGGCAGT ACACTGATAT TATCAGGTCT CTGCCCTTGA AGAGGCTTAC TGTGCGGTCC 
GCGCGGGTTC CTACTCAGAT TCTATTCGGA ACCCTGCGTG TGCTCGGATA TTCTGGCCTC 
CGGGAACTGA CTCTTGAAAA CCTCGAGGTA ACCGGCACCG CGCTTTCGCC GCTTCTGGAT 
GCCACCGGAC CCGATCTCAA CACCTTGAGC CTCCGCAACG TGTCGTGGGC AACAACGGAT 
ACCTGGCTCG CAGAACTGCA GCAGTGGCTA AAGCCTGGAC TCAAGGTACT AAGTATTGCC 
CAAGCACACT CACTCAACTT TTCTTGCAAA CAGGTCGGCG TCTTCCCTGC CCTTGCCACC 
TTAGATCTGT CTGACAACCC TGAGTTGGGC GAGAAAGGAC TGATCTCGGC CCTCTGTCCG 
CACAAATTCC CGACCCTCCA AGTTTTAGCG CTGCGCAACG CGGGGATGGA GACGACCAGC 
GGCGTGTGCT CTGCGCTGGC CGCAGCAAGA GTGCCACTGC AGGCACTGGA TCTCAGTCAC 
AATTCACTGC GGGATACTGC TGGCACTCCG AGCTGTGACT GGCCCAGTCA GCTAAACTCG 
CTCAATCTAT CTTTCACTGG GCTGGAGCAC GTACCTAAAG GGCTGCCAGC CAAGCTTAGC 
GTGCTTGATC TCAGTTACAA CAGGCTGGAT AGGAAACCTA GGCCAGAGGA GCTGCCTGAA 
GTGGGGAGCC TGTCACTTAC AGGAAATCCC TTTTTGCACT CTGAATCCCA GTCGGAGGCG 
TATAACTCTG GCGTAGTCAT AGCCACAGCT CTTTCACCCG GATCAGCGGG CTTGTCAGGA 
ACTTTGGCTT TGCTCCTAGG ACATCGCCTC TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd14 ( XM_006254603.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254665.1 Rattus norvegicus CD14 molecule (Cd14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38318
Clone ID Related Accession (Same CDS sequence)	XM_006254603.3
Accession Version	XM_006254603.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	monocyte differentiation antigen CD14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254603.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATGC TCGGCTTGTT GCTGTTGCCT TTGACACTGG TGCATGCCTC TCCCGCCACA 
CCAGAACCCT GTGAGCTGGA CCAGGACGAG GAAAGTGTCC GCTGCTACTG CAACTTCTCA 
GATCCGCAGC CCAATTGGTC CAGCGCTTTT CTATGCGCGG GGGCGGAAGA CGTGGAGTTC 
TACGGCGGCG GTCGCAGCCT GGAGTACCTT CTAAAGCGTG TGGACACGGA AGCAAATCTG 
GGGCAGTACA CTGATATTAT CAGGTCTCTG CCCTTGAAGA GGCTTACTGT GCGGTCCGCG 
CGGGTTCCTA CTCAGATTCT ATTCGGAACC CTGCGTGTGC TCGGATATTC TGGCCTCCGG 
GAACTGACTC TTGAAAACCT CGAGGTAACC GGCACCGCGC TTTCGCCGCT TCTGGATGCC 
ACCGGACCCG ATCTCAACAC CTTGAGCCTC CGCAACGTGT CGTGGGCAAC AACGGATACC 
TGGCTCGCAG AACTGCAGCA GTGGCTAAAG CCTGGACTCA AGGTACTAAG TATTGCCCAA 
GCACACTCAC TCAACTTTTC TTGCAAACAG GTCGGCGTCT TCCCTGCCCT TGCCACCTTA 
GATCTGTCTG ACAACCCTGA GTTGGGCGAG AAAGGACTGA TCTCGGCCCT CTGTCCGCAC 
AAATTCCCGA CCCTCCAAGT TTTAGCGCTG CGCAACGCGG GGATGGAGAC GACCAGCGGC 
GTGTGCTCTG CGCTGGCCGC AGCAAGAGTG CCACTGCAGG CACTGGATCT CAGTCACAAT 
TCACTGCGGG ATACTGCTGG CACTCCGAGC TGTGACTGGC CCAGTCAGCT AAACTCGCTC 
AATCTATCTT TCACTGGGCT GGAGCACGTA CCTAAAGGGC TGCCAGCCAA GCTTAGCGTG 
CTTGATCTCA GTTACAACAG GCTGGATAGG AAACCTAGGC CAGAGGAGCT GCCTGAAGTG 
GGGAGCCTGT CACTTACAGG AAATCCCTTT TTGCACTCTG AATCCCAGTC GGAGGCGTAT 
AACTCTGGCG TAGTCATAGC CACAGCTCTT TCACCCGGAT CAGCGGGCTT GTCAGGAACT 
TTGGCTTTGC TCCTAGGACA TCGCCTCTTT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254665.1
CDS	397..1512
Translation	MLMLGLLLLPLTLVHASPATPEPCELDQDEESVRCYCNFSDPQPNWSSAFLCAGAEDVEFYGGGRSLEYLLKRVDTEANLGQYTDIIRSLPLKRLTVRSARVPTQILFGTLRVLGYSGLRELTLENLEVTGTALSPLLDATGPDLNTLSLRNVSWATTDTWLAELQQWLKPGLKVLSIAQAHSLNFSCKQVGVFPALATLDLSDNPELGEKGLISALCPHKFPTLQVLALRNAGMETTSGVCSALAAARVPLQALDLSHNSLRDTAGTPSCDWPSQLNSLNLSFTGLEHVPKGLPAKLSVLDLSYNRLDRKPRPEELPEVGSLSLTGNPFLHSESQSEAYNSGVVIATALSPGSAGLSGTLALLLGHRLFV

Target ORF information:

RefSeq Version	XM_006254603.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD14 molecule (Cd14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254603.3

ORF Insert Sequence:

ATGCTTATGC TCGGCTTGTT GCTGTTGCCT TTGACACTGG TGCATGCCTC TCCCGCCACA 
CCAGAACCCT GTGAGCTGGA CCAGGACGAG GAAAGTGTCC GCTGCTACTG CAACTTCTCA 
GATCCGCAGC CCAATTGGTC CAGCGCTTTT CTATGCGCGG GGGCGGAAGA CGTGGAGTTC 
TACGGCGGCG GTCGCAGCCT GGAGTACCTT CTAAAGCGTG TGGACACGGA AGCAAATCTG 
GGGCAGTACA CTGATATTAT CAGGTCTCTG CCCTTGAAGA GGCTTACTGT GCGGTCCGCG 
CGGGTTCCTA CTCAGATTCT ATTCGGAACC CTGCGTGTGC TCGGATATTC TGGCCTCCGG 
GAACTGACTC TTGAAAACCT CGAGGTAACC GGCACCGCGC TTTCGCCGCT TCTGGATGCC 
ACCGGACCCG ATCTCAACAC CTTGAGCCTC CGCAACGTGT CGTGGGCAAC AACGGATACC 
TGGCTCGCAG AACTGCAGCA GTGGCTAAAG CCTGGACTCA AGGTACTAAG TATTGCCCAA 
GCACACTCAC TCAACTTTTC TTGCAAACAG GTCGGCGTCT TCCCTGCCCT TGCCACCTTA 
GATCTGTCTG ACAACCCTGA GTTGGGCGAG AAAGGACTGA TCTCGGCCCT CTGTCCGCAC 
AAATTCCCGA CCCTCCAAGT TTTAGCGCTG CGCAACGCGG GGATGGAGAC GACCAGCGGC 
GTGTGCTCTG CGCTGGCCGC AGCAAGAGTG CCACTGCAGG CACTGGATCT CAGTCACAAT 
TCACTGCGGG ATACTGCTGG CACTCCGAGC TGTGACTGGC CCAGTCAGCT AAACTCGCTC 
AATCTATCTT TCACTGGGCT GGAGCACGTA CCTAAAGGGC TGCCAGCCAA GCTTAGCGTG 
CTTGATCTCA GTTACAACAG GCTGGATAGG AAACCTAGGC CAGAGGAGCT GCCTGAAGTG 
GGGAGCCTGT CACTTACAGG AAATCCCTTT TTGCACTCTG AATCCCAGTC GGAGGCGTAT 
AACTCTGGCG TAGTCATAGC CACAGCTCTT TCACCCGGAT CAGCGGGCTT GTCAGGAACT 
TTGGCTTTGC TCCTAGGACA TCGCCTCTTT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Soluble CD14 inhibits contractile function and insulin action in primary adult rat cardiomyocytes.
Biochimica et biophysica acta1863(2)365-374(2017 Feb)
Overhagen S,Blumensatt M,Fahlbusch P,Herzfeld de Wiza D,M?ller H,Maxhera B,Akhyari P,Ouwens DM

The kinetic expression of lipopolysaccharide-binding protein and CD14 gene in obstructive jaundice.
Journal of investigative surgery : the official journal of the Academy of Surgical Research28(1)18-23(2015 Feb)
Tsai CH,Yeh CH,Sheen-Chen SM,Huang CY,Liu YW,Huang CC,Shen SC,Tang RP

The attenuated inflammation of MPL is due to the lack of CD14-dependent tight dimerization of the TLR4/MD2 complex at the plasma membrane.
International immunology26(6)307-14(2014 Jun)
Tanimura N,Saitoh S,Ohto U,Akashi-Takamura S,Fujimoto Y,Fukase K,Shimizu T,Miyake K

Effect of biliary drainage on inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats.
World journal of gastroenterology19(15)2319-30(2013 Apr)
Wang ZK,Xiao JG,Huang XF,Gong YC,Li W

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

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Cd14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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