LOC107131223 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107131223
Entrez Gene ID	107131223
Full Name	inactive ubiquitin carboxyl-terminal hydrolase 53
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003586178.4	XP_003586226.1	inactive ubiquitin carboxyl-terminal hydrolase 53
XM_015463686.1	XP_015319172.1	inactive ubiquitin carboxyl-terminal hydrolase 53
XM_003586178.5	XP_003586226.2	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_015463687.1	XP_015319173.1	inactive ubiquitin carboxyl-terminal hydrolase 53
XM_024993481.1	XP_024849249.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
XM_024993482.1	XP_024849250.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
XM_005207589.3	XP_005207646.1	inactive ubiquitin carboxyl-terminal hydrolase 53

Pathways from BioSystems
Gene Ontology

The following LOC107131223 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107131223 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa20142	XM_015463687.1 Latest version!	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa20142	XM_005207589.3 Latest version!	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa20142	XM_015463686.1 Latest version!	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa20142	XM_003586178.4 Latest version!	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa235473	XM_024993482.1 Latest version!	Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235471	XM_003586178.5 Latest version!	Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235472	XM_024993481.1 Latest version!	Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107131223 ( XM_003586178.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586226.1 Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20142
Clone ID Related Accession (Same CDS sequence)	XM_003586178.4 , XM_015463686.1 , XM_015463687.1 , XM_005207589.3
Accession Version	XM_003586178.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103959.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586178.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586226.1
CDS	773..1759
Translation	MAWVKFLRKPGGNLGKTYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSQEKALPSDNIRHALAESFRDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGMICYTSRHYCAFAFHTKSSKWVFFDDANVKEVSDT

Target ORF information:

RefSeq Version	XM_003586178.4
Organism	Bos taurus(cattle)
Definition	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586178.4

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131223 ( XM_015463686.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319172.1 Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20142
Clone ID Related Accession (Same CDS sequence)	XM_003586178.4 , XM_015463686.1 , XM_015463687.1 , XM_005207589.3
Accession Version	XM_015463686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319172.1
CDS	773..1759
Translation	MAWVKFLRKPGGNLGKTYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSQEKALPSDNIRHALAESFRDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGMICYTSRHYCAFAFHTKSSKWVFFDDANVKEVSDT

Target ORF information:

RefSeq Version	XM_015463686.1
Organism	Bos taurus(cattle)
Definition	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463686.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131223 ( XM_015463687.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319173.1 Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20142
Clone ID Related Accession (Same CDS sequence)	XM_003586178.4 , XM_015463686.1 , XM_015463687.1 , XM_005207589.3
Accession Version	XM_015463687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319173.1
CDS	959..1945
Translation	MAWVKFLRKPGGNLGKTYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSQEKALPSDNIRHALAESFRDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGMICYTSRHYCAFAFHTKSSKWVFFDDANVKEVSDT

Target ORF information:

RefSeq Version	XM_015463687.1
Organism	Bos taurus(cattle)
Definition	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463687.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131223 ( XM_005207589.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207646.1 Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20142
Clone ID Related Accession (Same CDS sequence)	XM_003586178.4 , XM_015463686.1 , XM_015463687.1 , XM_005207589.3
Accession Version	XM_005207589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103959.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207646.1
CDS	959..1945
Translation	MAWVKFLRKPGGNLGKTYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSQEKALPSDNIRHALAESFRDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGMICYTSRHYCAFAFHTKSSKWVFFDDANVKEVSDT

Target ORF information:

RefSeq Version	XM_005207589.3
Organism	Bos taurus(cattle)
Definition	Bos taurus inactive ubiquitin carboxyl-terminal hydrolase 53 (LOC107131223), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207589.3

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTAAGTGA CACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131223 ( XM_024993482.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849250.1 Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa235473
Clone ID Related Accession (Same CDS sequence)	XM_024993482.1
Accession Version	XM_024993482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACG CCGCAGAGTG CTTTGAAAAT ATATTGGAGA GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA AAAGTTTGCT 
ATGACTCTGT ATGAGCAGTG TGTGTGTCGT AGCTGTGGAG CATCATCAGA TCCTCTACCC 
TTTACAGAAT TTGTACGGTA CATTTCTACA ACAGCCCTGT GCAATGAAGT TGAAAGAATG 
ATGGAAAGAC ATGAGCGCTT TAAACCTGAT ATGTTTGCAG AATTGCTGCA AGCAGCAAAT 
ACAACTGATG ACTACAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGGCGT 
GTTTTAATGA ACTGCCCAGA GATTGTTACA ATCGGATTAG TCTGGGACTC TGAGCATTCT 
GACTTGACTG AAGATGTTGT TCGGAATCTG GCAACCCAGC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAGCTTC ACCTTGTTGG CATGATTTGC 
TACACCAGCC GACATTACTG TGCCTTTGCT TTTCATACCA AGAGTTCCAA ATGGGTATTT 
TTTGATGATG CAAATGTGAA AGAGGTTGGA AGTAGATGGA AAGATGTGGT CTCCAAGTGT 
ATCCGATGCC ATTTCCAGCC ATTGCTTTTG TTTTATGCGA ACCCAGATGG CACAGCAGTT 
TCCACTGAAG ATGCACTCAG GCAGGTCATC AACTGGTCAC ATCATAAATC TATTGCAGAA 
AATATAGGAT GTGAAAAGCC CTCGATTTCT AAGTCAGAAA ATTCAAAAGA AAATGGATTT 
GGTGATCAGA CAAAGCAGAG AGAAAATCAG AAATTTCAAG CAGATAGCAT TTCATCATTT 
AATCGGAGCC ACATTCAAAC AAGTGGCGGT AGAGGACCAG TTAAATCAGG TCACAATGAT 
CAAAGGGAAA AAATAAAAGA CATTTCCAGA GAATGTGCTC TAAAAGCCAT TGAACAGAAA 
AACTTACTTT CCTCACAAAG GAAAGATTTG GAGAGGGGAC AGAGAAAAGA TTTGGGTAGA 
CATAGAGATT TGGTTGATGA AGACCCTCCA CATTTCAAGA CTGGATCACC TCCTACCCTA 
AATGGCTTTA GACAGTATGG GAATTCACAT CTATATCACA GTCAAGGAAA AGGACCGTGT 
AAGCATGACC GAAATGCCCA TCAGAATCGA ACTTCTGCAC AAACCTTAAG TTCAAGTAAA 
TCCCAGATTC TTGCTTTGGG AGAGAAAATA ACTGGCAAAA TTAAGAGTGA TAGTGGTACT 
GGGTATGATA CAGACAGTAG CCAAGATTCT CGTGATAAAG GAAGCAGCTC TAACGGCAGC 
AATAAAAGCC GAAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT 
TTTAATGAAA GTGAAAAAAG GCAGCAGAGT CCAAGGCATA AATCAAATAT CAGTAACAAG 
TCTAAATGTA GCAAAGATCA GAGTTTTAAC GATTGGCCGA AAGAGAATCC AAAGCAAAAG 
TGTTTAATGA CTATATATGA AGATGAAATG AAGCAGGAAA CAGGAAGCAG GAGTTCCCTT 
GAATTTAATG GAAAAGTAGC AGAGAAAAAT AAAGCCCTCA AAGAAACTAA AGTTCATGGT 
GACAACTGGC AGATGCAAAG GACAGAGTCT GGATATGAAA GCAGTGATCA TATCAGTAAT 
GGCTCTGCTA ATTTGGACTC GCCTATTATT GATGGAAATG GTACACTGGT GGATATCAGT 
GGTGATAAAG AAACCACATC TTTCAGTGAT CAGATTAAGA CAAGCAACCT AAACACAGAA 
CGTGTGAACT ATACCTCCCA ACAGAGCAAA AATAACTTAG AAGGCTTTAA AAGAGAACTC 
AAGAACTTAG ATGTAGCCTC TAAAGCTCAT GAGTTCCACC CAGAATCACA TCTACAAATA 
AAAAATCCTT TGATAAAAAG GTCACATATA CATGAAACCA ATGGAAAGAC ACTCCCTTCA 
TCCAATCCAG AAATACTCAA GGACCACACA GTCAGAGAGC ATACCCACCA GTCAGATGAA 
CAGAAACTTG AAAAATCAAG TGAACCCAAG TTTTCTGAGT GGCTTAATAA AGAAAAGTCT 
GAGAAAACAG GTTTGCTTTT TCACACTGAT GATAACTCTG GTTCTGGAAA GAGAGTGAAC 
AGTAATGAAA CAGTCTCACC ATCTTCATCA TGGTCTTCAC ACATGAGAAC TGTGGGGTCA 
GAGCCAGAAA CTGCTCCCCT CATCCAGCAG CAAAACATGA TGGAACGATA TTACTCTGAG 
AACTCTCTAC CCAGGGAACA TCTGGTTCAG TCAACCTGTA GATCTGACGG CTGTCAGATG 
CTGAAACTTG TTTGCCAGAA TGCACCACCC CCTCTGCCAC CAAAGAAATA TGCTATGACC 
AGTGTGCCAC AGTCAGAGAA AAATGACTCT ACTCCTGATG TCAAAGTAAC AGAGACGTTT 
AAGACGAACT CTTCTAGTCT TCTGAAGCAC AGTCTAAGTA CAGCCTCAGA GCTAGGTGTA 
GACATGAGTC TGTATGTGAA CGATGAAAGA TTTAAGGATA CATTTCAAGC AAAAGAGTAT 
GTCAGCAACA CATCAAACAC TCCATCCAGT TCTGCAACTA AAGATTTTCA GGCAGACTCA 
GGTAGAGCTA TTGGTGCTTT CTGCCAGCCA GAAACAGACT CTAGTTCTAC ATGTCCAAAT 
GAGACAGTTT CATTAACTAC CTATTTTTCG GTTGATAACT GCATGACTGA TACATATAGA 
CTGAAATACC ATCAGAGGCC CAAGCTTTGC TTTCCAGAGA ACAGTGGCTT TTGTAATGAT 
AATTCACTGT CTCAGAATGA AAGAGAGCTA GGACCTGTGT TACATAGTAG TTTTGGTTCA 
AACTGCCCTT CTGAGCAAAA ACATAAACCT GGTATTTACT GTAATAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849250.1
CDS	3620..6610
Translation	MDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGMICYTSRHYCAFAFHTKSSKWVFFDDANVKEVGSRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVINWSHHKSIAENIGCEKPSISKSENSKENGFGDQTKQRENQKFQADSISSFNRSHIQTSGGRGPVKSGHNDQREKIKDISRECALKAIEQKNLLSSQRKDLERGQRKDLGRHRDLVDEDPPHFKTGSPPTLNGFRQYGNSHLYHSQGKGPCKHDRNAHQNRTSAQTLSSSKSQILALGEKITGKIKSDSGTGYDTDSSQDSRDKGSSSNGSNKSRNRGWKPMRETLNVDSIFNESEKRQQSPRHKSNISNKSKCSKDQSFNDWPKENPKQKCLMTIYEDEMKQETGSRSSLEFNGKVAEKNKALKETKVHGDNWQMQRTESGYESSDHISNGSANLDSPIIDGNGTLVDISGDKETTSFSDQIKTSNLNTERVNYTSQQSKNNLEGFKRELKNLDVASKAHEFHPESHLQIKNPLIKRSHIHETNGKTLPSSNPEILKDHTVREHTHQSDEQKLEKSSEPKFSEWLNKEKSEKTGLLFHTDDNSGSGKRVNSNETVSPSSSWSSHMRTVGSEPETAPLIQQQNMMERYYSENSLPREHLVQSTCRSDGCQMLKLVCQNAPPPLPPKKYAMTSVPQSEKNDSTPDVKVTETFKTNSSSLLKHSLSTASELGVDMSLYVNDERFKDTFQAKEYVSNTSNTPSSSATKDFQADSGRAIGAFCQPETDSSSTCPNETVSLTTYFSVDNCMTDTYRLKYHQRPKLCFPENSGFCNDNSLSQNERELGPVLHSSFGSNCPSEQKHKPGIYCNR

Target ORF information:

RefSeq Version	XM_024993482.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993482.1

ORF Insert Sequence:

ATGGACGACG CCGCAGAGTG CTTTGAAAAT ATATTGGAGA GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA AAAGTTTGCT 
ATGACTCTGT ATGAGCAGTG TGTGTGTCGT AGCTGTGGAG CATCATCAGA TCCTCTACCC 
TTTACAGAAT TTGTACGGTA CATTTCTACA ACAGCCCTGT GCAATGAAGT TGAAAGAATG 
ATGGAAAGAC ATGAGCGCTT TAAACCTGAT ATGTTTGCAG AATTGCTGCA AGCAGCAAAT 
ACAACTGATG ACTACAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGGCGT 
GTTTTAATGA ACTGCCCAGA GATTGTTACA ATCGGATTAG TCTGGGACTC TGAGCATTCT 
GACTTGACTG AAGATGTTGT TCGGAATCTG GCAACCCAGC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAGCTTC ACCTTGTTGG CATGATTTGC 
TACACCAGCC GACATTACTG TGCCTTTGCT TTTCATACCA AGAGTTCCAA ATGGGTATTT 
TTTGATGATG CAAATGTGAA AGAGGTTGGA AGTAGATGGA AAGATGTGGT CTCCAAGTGT 
ATCCGATGCC ATTTCCAGCC ATTGCTTTTG TTTTATGCGA ACCCAGATGG CACAGCAGTT 
TCCACTGAAG ATGCACTCAG GCAGGTCATC AACTGGTCAC ATCATAAATC TATTGCAGAA 
AATATAGGAT GTGAAAAGCC CTCGATTTCT AAGTCAGAAA ATTCAAAAGA AAATGGATTT 
GGTGATCAGA CAAAGCAGAG AGAAAATCAG AAATTTCAAG CAGATAGCAT TTCATCATTT 
AATCGGAGCC ACATTCAAAC AAGTGGCGGT AGAGGACCAG TTAAATCAGG TCACAATGAT 
CAAAGGGAAA AAATAAAAGA CATTTCCAGA GAATGTGCTC TAAAAGCCAT TGAACAGAAA 
AACTTACTTT CCTCACAAAG GAAAGATTTG GAGAGGGGAC AGAGAAAAGA TTTGGGTAGA 
CATAGAGATT TGGTTGATGA AGACCCTCCA CATTTCAAGA CTGGATCACC TCCTACCCTA 
AATGGCTTTA GACAGTATGG GAATTCACAT CTATATCACA GTCAAGGAAA AGGACCGTGT 
AAGCATGACC GAAATGCCCA TCAGAATCGA ACTTCTGCAC AAACCTTAAG TTCAAGTAAA 
TCCCAGATTC TTGCTTTGGG AGAGAAAATA ACTGGCAAAA TTAAGAGTGA TAGTGGTACT 
GGGTATGATA CAGACAGTAG CCAAGATTCT CGTGATAAAG GAAGCAGCTC TAACGGCAGC 
AATAAAAGCC GAAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT 
TTTAATGAAA GTGAAAAAAG GCAGCAGAGT CCAAGGCATA AATCAAATAT CAGTAACAAG 
TCTAAATGTA GCAAAGATCA GAGTTTTAAC GATTGGCCGA AAGAGAATCC AAAGCAAAAG 
TGTTTAATGA CTATATATGA AGATGAAATG AAGCAGGAAA CAGGAAGCAG GAGTTCCCTT 
GAATTTAATG GAAAAGTAGC AGAGAAAAAT AAAGCCCTCA AAGAAACTAA AGTTCATGGT 
GACAACTGGC AGATGCAAAG GACAGAGTCT GGATATGAAA GCAGTGATCA TATCAGTAAT 
GGCTCTGCTA ATTTGGACTC GCCTATTATT GATGGAAATG GTACACTGGT GGATATCAGT 
GGTGATAAAG AAACCACATC TTTCAGTGAT CAGATTAAGA CAAGCAACCT AAACACAGAA 
CGTGTGAACT ATACCTCCCA ACAGAGCAAA AATAACTTAG AAGGCTTTAA AAGAGAACTC 
AAGAACTTAG ATGTAGCCTC TAAAGCTCAT GAGTTCCACC CAGAATCACA TCTACAAATA 
AAAAATCCTT TGATAAAAAG GTCACATATA CATGAAACCA ATGGAAAGAC ACTCCCTTCA 
TCCAATCCAG AAATACTCAA GGACCACACA GTCAGAGAGC ATACCCACCA GTCAGATGAA 
CAGAAACTTG AAAAATCAAG TGAACCCAAG TTTTCTGAGT GGCTTAATAA AGAAAAGTCT 
GAGAAAACAG GTTTGCTTTT TCACACTGAT GATAACTCTG GTTCTGGAAA GAGAGTGAAC 
AGTAATGAAA CAGTCTCACC ATCTTCATCA TGGTCTTCAC ACATGAGAAC TGTGGGGTCA 
GAGCCAGAAA CTGCTCCCCT CATCCAGCAG CAAAACATGA TGGAACGATA TTACTCTGAG 
AACTCTCTAC CCAGGGAACA TCTGGTTCAG TCAACCTGTA GATCTGACGG CTGTCAGATG 
CTGAAACTTG TTTGCCAGAA TGCACCACCC CCTCTGCCAC CAAAGAAATA TGCTATGACC 
AGTGTGCCAC AGTCAGAGAA AAATGACTCT ACTCCTGATG TCAAAGTAAC AGAGACGTTT 
AAGACGAACT CTTCTAGTCT TCTGAAGCAC AGTCTAAGTA CAGCCTCAGA GCTAGGTGTA 
GACATGAGTC TGTATGTGAA CGATGAAAGA TTTAAGGATA CATTTCAAGC AAAAGAGTAT 
GTCAGCAACA CATCAAACAC TCCATCCAGT TCTGCAACTA AAGATTTTCA GGCAGACTCA 
GGTAGAGCTA TTGGTGCTTT CTGCCAGCCA GAAACAGACT CTAGTTCTAC ATGTCCAAAT 
GAGACAGTTT CATTAACTAC CTATTTTTCG GTTGATAACT GCATGACTGA TACATATAGA 
CTGAAATACC ATCAGAGGCC CAAGCTTTGC TTTCCAGAGA ACAGTGGCTT TTGTAATGAT 
AATTCACTGT CTCAGAATGA AAGAGAGCTA GGACCTGTGT TACATAGTAG TTTTGGTTCA 
AACTGCCCTT CTGAGCAAAA ACATAAACCT GGTATTTACT GTAATAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131223 ( XM_003586178.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586226.2 Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235471
Clone ID Related Accession (Same CDS sequence)	XM_003586178.5
Accession Version	XM_003586178.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586178.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTTGGAAG TAGATGGAAA GATGTGGTCT CCAAGTGTAT CCGATGCCAT 
TTCCAGCCAT TGCTTTTGTT TTATGCGAAC CCAGATGGCA CAGCAGTTTC CACTGAAGAT 
GCACTCAGGC AGGTCATCAA CTGGTCACAT CATAAATCTA TTGCAGAAAA TATAGGATGT 
GAAAAGCCCT CGATTTCTAA GTCAGAAAAT TCAAAAGAAA ATGGATTTGG TGATCAGACA 
AAGCAGAGAG AAAATCAGAA ATTTCAAGCA GATAGCATTT CATCATTTAA TCGGAGCCAC 
ATTCAAACAA GTGGCGGTAG AGGACCAGTT AAATCAGGTC ACAATGATCA AAGGGAAAAA 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTA AAAGCCATTG AACAGAAAAA CTTACTTTCC 
TCACAAAGGA AAGATTTGGA GAGGGGACAG AGAAAAGATT TGGGTAGACA TAGAGATTTG 
GTTGATGAAG ACCCTCCACA TTTCAAGACT GGATCACCTC CTACCCTAAA TGGCTTTAGA 
CAGTATGGGA ATTCACATCT ATATCACAGT CAAGGAAAAG GACCGTGTAA GCATGACCGA 
AATGCCCATC AGAATCGAAC TTCTGCACAA ACCTTAAGTT CAAGTAAATC CCAGATTCTT 
GCTTTGGGAG AGAAAATAAC TGGCAAAATT AAGAGTGATA GTGGTACTGG GTATGATACA 
GACAGTAGCC AAGATTCTCG TGATAAAGGA AGCAGCTCTA ACGGCAGCAA TAAAAGCCGA 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAATGAAAGT 
GAAAAAAGGC AGCAGAGTCC AAGGCATAAA TCAAATATCA GTAACAAGTC TAAATGTAGC 
AAAGATCAGA GTTTTAACGA TTGGCCGAAA GAGAATCCAA AGCAAAAGTG TTTAATGACT 
ATATATGAAG ATGAAATGAA GCAGGAAACA GGAAGCAGGA GTTCCCTTGA ATTTAATGGA 
AAAGTAGCAG AGAAAAATAA AGCCCTCAAA GAAACTAAAG TTCATGGTGA CAACTGGCAG 
ATGCAAAGGA CAGAGTCTGG ATATGAAAGC AGTGATCATA TCAGTAATGG CTCTGCTAAT 
TTGGACTCGC CTATTATTGA TGGAAATGGT ACACTGGTGG ATATCAGTGG TGATAAAGAA 
ACCACATCTT TCAGTGATCA GATTAAGACA AGCAACCTAA ACACAGAACG TGTGAACTAT 
ACCTCCCAAC AGAGCAAAAA TAACTTAGAA GGCTTTAAAA GAGAACTCAA GAACTTAGAT 
GTAGCCTCTA AAGCTCATGA GTTCCACCCA GAATCACATC TACAAATAAA AAATCCTTTG 
ATAAAAAGGT CACATATACA TGAAACCAAT GGAAAGACAC TCCCTTCATC CAATCCAGAA 
ATACTCAAGG ACCACACAGT CAGAGAGCAT ACCCACCAGT CAGATGAACA GAAACTTGAA 
AAATCAAGTG AACCCAAGTT TTCTGAGTGG CTTAATAAAG AAAAGTCTGA GAAAACAGGT 
TTGCTTTTTC ACACTGATGA TAACTCTGGT TCTGGAAAGA GAGTGAACAG TAATGAAACA 
GTCTCACCAT CTTCATCATG GTCTTCACAC ATGAGAACTG TGGGGTCAGA GCCAGAAACT 
GCTCCCCTCA TCCAGCAGCA AAACATGATG GAACGATATT ACTCTGAGAA CTCTCTACCC 
AGGGAACATC TGGTTCAGTC AACCTGTAGA TCTGACGGCT GTCAGATGCT GAAACTTGTT 
TGCCAGAATG CACCACCCCC TCTGCCACCA AAGAAATATG CTATGACCAG TGTGCCACAG 
TCAGAGAAAA ATGACTCTAC TCCTGATGTC AAAGTAACAG AGACGTTTAA GACGAACTCT 
TCTAGTCTTC TGAAGCACAG TCTAAGTACA GCCTCAGAGC TAGGTGTAGA CATGAGTCTG 
TATGTGAACG ATGAAAGATT TAAGGATACA TTTCAAGCAA AAGAGTATGT CAGCAACACA 
TCAAACACTC CATCCAGTTC TGCAACTAAA GATTTTCAGG CAGACTCAGG TAGAGCTATT 
GGTGCTTTCT GCCAGCCAGA AACAGACTCT AGTTCTACAT GTCCAAATGA GACAGTTTCA 
TTAACTACCT ATTTTTCGGT TGATAACTGC ATGACTGATA CATATAGACT GAAATACCAT 
CAGAGGCCCA AGCTTTGCTT TCCAGAGAAC AGTGGCTTTT GTAATGATAA TTCACTGTCT 
CAGAATGAAA GAGAGCTAGG ACCTGTGTTA CATAGTAGTT TTGGTTCAAA CTGCCCTTCT 
GAGCAAAAAC ATAAACCTGG TATTTACTGT AATAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586226.2
CDS	838..4176
Translation	MAWVKFLRKPGGNLGKTYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSQEKALPSDNIRHALAESFRDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGMICYTSRHYCAFAFHTKSSKWVFFDDANVKEVGSRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVINWSHHKSIAENIGCEKPSISKSENSKENGFGDQTKQRENQKFQADSISSFNRSHIQTSGGRGPVKSGHNDQREKIKDISRECALKAIEQKNLLSSQRKDLERGQRKDLGRHRDLVDEDPPHFKTGSPPTLNGFRQYGNSHLYHSQGKGPCKHDRNAHQNRTSAQTLSSSKSQILALGEKITGKIKSDSGTGYDTDSSQDSRDKGSSSNGSNKSRNRGWKPMRETLNVDSIFNESEKRQQSPRHKSNISNKSKCSKDQSFNDWPKENPKQKCLMTIYEDEMKQETGSRSSLEFNGKVAEKNKALKETKVHGDNWQMQRTESGYESSDHISNGSANLDSPIIDGNGTLVDISGDKETTSFSDQIKTSNLNTERVNYTSQQSKNNLEGFKRELKNLDVASKAHEFHPESHLQIKNPLIKRSHIHETNGKTLPSSNPEILKDHTVREHTHQSDEQKLEKSSEPKFSEWLNKEKSEKTGLLFHTDDNSGSGKRVNSNETVSPSSSWSSHMRTVGSEPETAPLIQQQNMMERYYSENSLPREHLVQSTCRSDGCQMLKLVCQNAPPPLPPKKYAMTSVPQSEKNDSTPDVKVTETFKTNSSSLLKHSLSTASELGVDMSLYVNDERFKDTFQAKEYVSNTSNTPSSSATKDFQADSGRAIGAFCQPETDSSSTCPNETVSLTTYFSVDNCMTDTYRLKYHQRPKLCFPENSGFCNDNSLSQNERELGPVLHSSFGSNCPSEQKHKPGIYCNR

Target ORF information:

RefSeq Version	XM_003586178.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586178.5

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAGCTTCAC CTTGTTGGCA TGATTTGCTA CACCAGCCGA 
CATTACTGTG CCTTTGCTTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGGTTGGAAG TAGATGGAAA GATGTGGTCT CCAAGTGTAT CCGATGCCAT 
TTCCAGCCAT TGCTTTTGTT TTATGCGAAC CCAGATGGCA CAGCAGTTTC CACTGAAGAT 
GCACTCAGGC AGGTCATCAA CTGGTCACAT CATAAATCTA TTGCAGAAAA TATAGGATGT 
GAAAAGCCCT CGATTTCTAA GTCAGAAAAT TCAAAAGAAA ATGGATTTGG TGATCAGACA 
AAGCAGAGAG AAAATCAGAA ATTTCAAGCA GATAGCATTT CATCATTTAA TCGGAGCCAC 
ATTCAAACAA GTGGCGGTAG AGGACCAGTT AAATCAGGTC ACAATGATCA AAGGGAAAAA 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTA AAAGCCATTG AACAGAAAAA CTTACTTTCC 
TCACAAAGGA AAGATTTGGA GAGGGGACAG AGAAAAGATT TGGGTAGACA TAGAGATTTG 
GTTGATGAAG ACCCTCCACA TTTCAAGACT GGATCACCTC CTACCCTAAA TGGCTTTAGA 
CAGTATGGGA ATTCACATCT ATATCACAGT CAAGGAAAAG GACCGTGTAA GCATGACCGA 
AATGCCCATC AGAATCGAAC TTCTGCACAA ACCTTAAGTT CAAGTAAATC CCAGATTCTT 
GCTTTGGGAG AGAAAATAAC TGGCAAAATT AAGAGTGATA GTGGTACTGG GTATGATACA 
GACAGTAGCC AAGATTCTCG TGATAAAGGA AGCAGCTCTA ACGGCAGCAA TAAAAGCCGA 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAATGAAAGT 
GAAAAAAGGC AGCAGAGTCC AAGGCATAAA TCAAATATCA GTAACAAGTC TAAATGTAGC 
AAAGATCAGA GTTTTAACGA TTGGCCGAAA GAGAATCCAA AGCAAAAGTG TTTAATGACT 
ATATATGAAG ATGAAATGAA GCAGGAAACA GGAAGCAGGA GTTCCCTTGA ATTTAATGGA 
AAAGTAGCAG AGAAAAATAA AGCCCTCAAA GAAACTAAAG TTCATGGTGA CAACTGGCAG 
ATGCAAAGGA CAGAGTCTGG ATATGAAAGC AGTGATCATA TCAGTAATGG CTCTGCTAAT 
TTGGACTCGC CTATTATTGA TGGAAATGGT ACACTGGTGG ATATCAGTGG TGATAAAGAA 
ACCACATCTT TCAGTGATCA GATTAAGACA AGCAACCTAA ACACAGAACG TGTGAACTAT 
ACCTCCCAAC AGAGCAAAAA TAACTTAGAA GGCTTTAAAA GAGAACTCAA GAACTTAGAT 
GTAGCCTCTA AAGCTCATGA GTTCCACCCA GAATCACATC TACAAATAAA AAATCCTTTG 
ATAAAAAGGT CACATATACA TGAAACCAAT GGAAAGACAC TCCCTTCATC CAATCCAGAA 
ATACTCAAGG ACCACACAGT CAGAGAGCAT ACCCACCAGT CAGATGAACA GAAACTTGAA 
AAATCAAGTG AACCCAAGTT TTCTGAGTGG CTTAATAAAG AAAAGTCTGA GAAAACAGGT 
TTGCTTTTTC ACACTGATGA TAACTCTGGT TCTGGAAAGA GAGTGAACAG TAATGAAACA 
GTCTCACCAT CTTCATCATG GTCTTCACAC ATGAGAACTG TGGGGTCAGA GCCAGAAACT 
GCTCCCCTCA TCCAGCAGCA AAACATGATG GAACGATATT ACTCTGAGAA CTCTCTACCC 
AGGGAACATC TGGTTCAGTC AACCTGTAGA TCTGACGGCT GTCAGATGCT GAAACTTGTT 
TGCCAGAATG CACCACCCCC TCTGCCACCA AAGAAATATG CTATGACCAG TGTGCCACAG 
TCAGAGAAAA ATGACTCTAC TCCTGATGTC AAAGTAACAG AGACGTTTAA GACGAACTCT 
TCTAGTCTTC TGAAGCACAG TCTAAGTACA GCCTCAGAGC TAGGTGTAGA CATGAGTCTG 
TATGTGAACG ATGAAAGATT TAAGGATACA TTTCAAGCAA AAGAGTATGT CAGCAACACA 
TCAAACACTC CATCCAGTTC TGCAACTAAA GATTTTCAGG CAGACTCAGG TAGAGCTATT 
GGTGCTTTCT GCCAGCCAGA AACAGACTCT AGTTCTACAT GTCCAAATGA GACAGTTTCA 
TTAACTACCT ATTTTTCGGT TGATAACTGC ATGACTGATA CATATAGACT GAAATACCAT 
CAGAGGCCCA AGCTTTGCTT TCCAGAGAAC AGTGGCTTTT GTAATGATAA TTCACTGTCT 
CAGAATGAAA GAGAGCTAGG ACCTGTGTTA CATAGTAGTT TTGGTTCAAA CTGCCCTTCT 
GAGCAAAAAC ATAAACCTGG TATTTACTGT AATAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131223 ( XM_024993481.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849249.1 Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

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CloneID	OBa235472
Clone ID Related Accession (Same CDS sequence)	XM_024993481.1
Accession Version	XM_024993481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGGTTGGAAG TAGATGGAAA 
GATGTGGTCT CCAAGTGTAT CCGATGCCAT TTCCAGCCAT TGCTTTTGTT TTATGCGAAC 
CCAGATGGCA CAGCAGTTTC CACTGAAGAT GCACTCAGGC AGGTCATCAA CTGGTCACAT 
CATAAATCTA TTGCAGAAAA TATAGGATGT GAAAAGCCCT CGATTTCTAA GTCAGAAAAT 
TCAAAAGAAA ATGGATTTGG TGATCAGACA AAGCAGAGAG AAAATCAGAA ATTTCAAGCA 
GATAGCATTT CATCATTTAA TCGGAGCCAC ATTCAAACAA GTGGCGGTAG AGGACCAGTT 
AAATCAGGTC ACAATGATCA AAGGGAAAAA ATAAAAGACA TTTCCAGAGA ATGTGCTCTA 
AAAGCCATTG AACAGAAAAA CTTACTTTCC TCACAAAGGA AAGATTTGGA GAGGGGACAG 
AGAAAAGATT TGGGTAGACA TAGAGATTTG GTTGATGAAG ACCCTCCACA TTTCAAGACT 
GGATCACCTC CTACCCTAAA TGGCTTTAGA CAGTATGGGA ATTCACATCT ATATCACAGT 
CAAGGAAAAG GACCGTGTAA GCATGACCGA AATGCCCATC AGAATCGAAC TTCTGCACAA 
ACCTTAAGTT CAAGTAAATC CCAGATTCTT GCTTTGGGAG AGAAAATAAC TGGCAAAATT 
AAGAGTGATA GTGGTACTGG GTATGATACA GACAGTAGCC AAGATTCTCG TGATAAAGGA 
AGCAGCTCTA ACGGCAGCAA TAAAAGCCGA AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ATAGTATTTT TAATGAAAGT GAAAAAAGGC AGCAGAGTCC AAGGCATAAA 
TCAAATATCA GTAACAAGTC TAAATGTAGC AAAGATCAGA GTTTTAACGA TTGGCCGAAA 
GAGAATCCAA AGCAAAAGTG TTTAATGACT ATATATGAAG ATGAAATGAA GCAGGAAACA 
GGAAGCAGGA GTTCCCTTGA ATTTAATGGA AAAGTAGCAG AGAAAAATAA AGCCCTCAAA 
GAAACTAAAG TTCATGGTGA CAACTGGCAG ATGCAAAGGA CAGAGTCTGG ATATGAAAGC 
AGTGATCATA TCAGTAATGG CTCTGCTAAT TTGGACTCGC CTATTATTGA TGGAAATGGT 
ACACTGGTGG ATATCAGTGG TGATAAAGAA ACCACATCTT TCAGTGATCA GATTAAGACA 
AGCAACCTAA ACACAGAACG TGTGAACTAT ACCTCCCAAC AGAGCAAAAA TAACTTAGAA 
GGCTTTAAAA GAGAACTCAA GAACTTAGAT GTAGCCTCTA AAGCTCATGA GTTCCACCCA 
GAATCACATC TACAAATAAA AAATCCTTTG ATAAAAAGGT CACATATACA TGAAACCAAT 
GGAAAGACAC TCCCTTCATC CAATCCAGAA ATACTCAAGG ACCACACAGT CAGAGAGCAT 
ACCCACCAGT CAGATGAACA GAAACTTGAA AAATCAAGTG AACCCAAGTT TTCTGAGTGG 
CTTAATAAAG AAAAGTCTGA GAAAACAGGT TTGCTTTTTC ACACTGATGA TAACTCTGGT 
TCTGGAAAGA GAGTGAACAG TAATGAAACA GTCTCACCAT CTTCATCATG GTCTTCACAC 
ATGAGAACTG TGGGGTCAGA GCCAGAAACT GCTCCCCTCA TCCAGCAGCA AAACATGATG 
GAACGATATT ACTCTGAGAA CTCTCTACCC AGGGAACATC TGGTTCAGTC AACCTGTAGA 
TCTGACGGCT GTCAGATGCT GAAACTTGTT TGCCAGAATG CACCACCCCC TCTGCCACCA 
AAGAAATATG CTATGACCAG TGTGCCACAG TCAGAGAAAA ATGACTCTAC TCCTGATGTC 
AAAGTAACAG AGACGTTTAA GACGAACTCT TCTAGTCTTC TGAAGCACAG TCTAAGTACA 
GCCTCAGAGC TAGGTGTAGA CATGAGTCTG TATGTGAACG ATGAAAGATT TAAGGATACA 
TTTCAAGCAA AAGAGTATGT CAGCAACACA TCAAACACTC CATCCAGTTC TGCAACTAAA 
GATTTTCAGG CAGACTCAGG TAGAGCTATT GGTGCTTTCT GCCAGCCAGA AACAGACTCT 
AGTTCTACAT GTCCAAATGA GACAGTTTCA TTAACTACCT ATTTTTCGGT TGATAACTGC 
ATGACTGATA CATATAGACT GAAATACCAT CAGAGGCCCA AGCTTTGCTT TCCAGAGAAC 
AGTGGCTTTT GTAATGATAA TTCACTGTCT CAGAATGAAA GAGAGCTAGG ACCTGTGTTA 
CATAGTAGTT TTGGTTCAAA CTGCCCTTCT GAGCAAAAAC ATAAACCTGG TATTTACTGT 
AATAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849249.1
CDS	777..3965
Translation	MAWVKFLRKPGGNLGKTYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSQEKALPSDNIRHALAESFRDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERFKPDMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVVRNLATQLYLPGVGSRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVINWSHHKSIAENIGCEKPSISKSENSKENGFGDQTKQRENQKFQADSISSFNRSHIQTSGGRGPVKSGHNDQREKIKDISRECALKAIEQKNLLSSQRKDLERGQRKDLGRHRDLVDEDPPHFKTGSPPTLNGFRQYGNSHLYHSQGKGPCKHDRNAHQNRTSAQTLSSSKSQILALGEKITGKIKSDSGTGYDTDSSQDSRDKGSSSNGSNKSRNRGWKPMRETLNVDSIFNESEKRQQSPRHKSNISNKSKCSKDQSFNDWPKENPKQKCLMTIYEDEMKQETGSRSSLEFNGKVAEKNKALKETKVHGDNWQMQRTESGYESSDHISNGSANLDSPIIDGNGTLVDISGDKETTSFSDQIKTSNLNTERVNYTSQQSKNNLEGFKRELKNLDVASKAHEFHPESHLQIKNPLIKRSHIHETNGKTLPSSNPEILKDHTVREHTHQSDEQKLEKSSEPKFSEWLNKEKSEKTGLLFHTDDNSGSGKRVNSNETVSPSSSWSSHMRTVGSEPETAPLIQQQNMMERYYSENSLPREHLVQSTCRSDGCQMLKLVCQNAPPPLPPKKYAMTSVPQSEKNDSTPDVKVTETFKTNSSSLLKHSLSTASELGVDMSLYVNDERFKDTFQAKEYVSNTSNTPSSSATKDFQADSGRAIGAFCQPETDSSSTCPNETVSLTTYFSVDNCMTDTYRLKYHQRPKLCFPENSGFCNDNSLSQNERELGPVLHSSFGSNCPSEQKHKPGIYCNR

Target ORF information:

RefSeq Version	XM_024993481.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993481.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TCGGAAAAAC TTATCAGCCT 
GGGAGTATGC TATCCCTGGC CCCTACCAAA GGCTTGTTGA ATGAACCAGG ACAAAACAGC 
TGCTTTCTCA ACAGTGCTGT ACAGGTTTTA TGGCAATTAG ATATATTCCG AAGAAGCTTG 
CGGGTGCTGA CTGGACATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTGAAGACA 
ATATTTGCAC AGTTTCAACA CAGTCAAGAG AAAGCCCTCC CCTCGGATAA CATAAGGCAT 
GCTCTTGCAG AGAGCTTCAG AGACGAGCAG CGGTTTCAGC TGGGCCTGAT GGACGACGCC 
GCAGAGTGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAAA AGTTTGCTAT GACTCTGTAT 
GAGCAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCCTT TACAGAATTT 
GTACGGTACA TTTCTACAAC AGCCCTGTGC AATGAAGTTG AAAGAATGAT GGAAAGACAT 
GAGCGCTTTA AACCTGATAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC 
TACAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGGCGTGT TTTAATGAAC 
TGCCCAGAGA TTGTTACAAT CGGATTAGTC TGGGACTCTG AGCATTCTGA CTTGACTGAA 
GATGTTGTTC GGAATCTGGC AACCCAGCTT TATCTTCCTG GGGTTGGAAG TAGATGGAAA 
GATGTGGTCT CCAAGTGTAT CCGATGCCAT TTCCAGCCAT TGCTTTTGTT TTATGCGAAC 
CCAGATGGCA CAGCAGTTTC CACTGAAGAT GCACTCAGGC AGGTCATCAA CTGGTCACAT 
CATAAATCTA TTGCAGAAAA TATAGGATGT GAAAAGCCCT CGATTTCTAA GTCAGAAAAT 
TCAAAAGAAA ATGGATTTGG TGATCAGACA AAGCAGAGAG AAAATCAGAA ATTTCAAGCA 
GATAGCATTT CATCATTTAA TCGGAGCCAC ATTCAAACAA GTGGCGGTAG AGGACCAGTT 
AAATCAGGTC ACAATGATCA AAGGGAAAAA ATAAAAGACA TTTCCAGAGA ATGTGCTCTA 
AAAGCCATTG AACAGAAAAA CTTACTTTCC TCACAAAGGA AAGATTTGGA GAGGGGACAG 
AGAAAAGATT TGGGTAGACA TAGAGATTTG GTTGATGAAG ACCCTCCACA TTTCAAGACT 
GGATCACCTC CTACCCTAAA TGGCTTTAGA CAGTATGGGA ATTCACATCT ATATCACAGT 
CAAGGAAAAG GACCGTGTAA GCATGACCGA AATGCCCATC AGAATCGAAC TTCTGCACAA 
ACCTTAAGTT CAAGTAAATC CCAGATTCTT GCTTTGGGAG AGAAAATAAC TGGCAAAATT 
AAGAGTGATA GTGGTACTGG GTATGATACA GACAGTAGCC AAGATTCTCG TGATAAAGGA 
AGCAGCTCTA ACGGCAGCAA TAAAAGCCGA AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ATAGTATTTT TAATGAAAGT GAAAAAAGGC AGCAGAGTCC AAGGCATAAA 
TCAAATATCA GTAACAAGTC TAAATGTAGC AAAGATCAGA GTTTTAACGA TTGGCCGAAA 
GAGAATCCAA AGCAAAAGTG TTTAATGACT ATATATGAAG ATGAAATGAA GCAGGAAACA 
GGAAGCAGGA GTTCCCTTGA ATTTAATGGA AAAGTAGCAG AGAAAAATAA AGCCCTCAAA 
GAAACTAAAG TTCATGGTGA CAACTGGCAG ATGCAAAGGA CAGAGTCTGG ATATGAAAGC 
AGTGATCATA TCAGTAATGG CTCTGCTAAT TTGGACTCGC CTATTATTGA TGGAAATGGT 
ACACTGGTGG ATATCAGTGG TGATAAAGAA ACCACATCTT TCAGTGATCA GATTAAGACA 
AGCAACCTAA ACACAGAACG TGTGAACTAT ACCTCCCAAC AGAGCAAAAA TAACTTAGAA 
GGCTTTAAAA GAGAACTCAA GAACTTAGAT GTAGCCTCTA AAGCTCATGA GTTCCACCCA 
GAATCACATC TACAAATAAA AAATCCTTTG ATAAAAAGGT CACATATACA TGAAACCAAT 
GGAAAGACAC TCCCTTCATC CAATCCAGAA ATACTCAAGG ACCACACAGT CAGAGAGCAT 
ACCCACCAGT CAGATGAACA GAAACTTGAA AAATCAAGTG AACCCAAGTT TTCTGAGTGG 
CTTAATAAAG AAAAGTCTGA GAAAACAGGT TTGCTTTTTC ACACTGATGA TAACTCTGGT 
TCTGGAAAGA GAGTGAACAG TAATGAAACA GTCTCACCAT CTTCATCATG GTCTTCACAC 
ATGAGAACTG TGGGGTCAGA GCCAGAAACT GCTCCCCTCA TCCAGCAGCA AAACATGATG 
GAACGATATT ACTCTGAGAA CTCTCTACCC AGGGAACATC TGGTTCAGTC AACCTGTAGA 
TCTGACGGCT GTCAGATGCT GAAACTTGTT TGCCAGAATG CACCACCCCC TCTGCCACCA 
AAGAAATATG CTATGACCAG TGTGCCACAG TCAGAGAAAA ATGACTCTAC TCCTGATGTC 
AAAGTAACAG AGACGTTTAA GACGAACTCT TCTAGTCTTC TGAAGCACAG TCTAAGTACA 
GCCTCAGAGC TAGGTGTAGA CATGAGTCTG TATGTGAACG ATGAAAGATT TAAGGATACA 
TTTCAAGCAA AAGAGTATGT CAGCAACACA TCAAACACTC CATCCAGTTC TGCAACTAAA 
GATTTTCAGG CAGACTCAGG TAGAGCTATT GGTGCTTTCT GCCAGCCAGA AACAGACTCT 
AGTTCTACAT GTCCAAATGA GACAGTTTCA TTAACTACCT ATTTTTCGGT TGATAACTGC 
ATGACTGATA CATATAGACT GAAATACCAT CAGAGGCCCA AGCTTTGCTT TCCAGAGAAC 
AGTGGCTTTT GTAATGATAA TTCACTGTCT CAGAATGAAA GAGAGCTAGG ACCTGTGTTA 
CATAGTAGTT TTGGTTCAAA CTGCCCTTCT GAGCAAAAAC ATAAACCTGG TATTTACTGT 
AATAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC107131223 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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