FRAS1 cDNA ORF clone, Bos taurus(cattle)

The following FRAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FRAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235464 XM_024993475.1
Latest version!
Bos taurus Fraser extracellular matrix complex subunit 1 (FRAS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90112 XM_015463814.1
Latest version!
Bos taurus extracellular matrix protein FRAS1-like (LOC107131184), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90954 XM_015471687.1
Latest version!
Bos taurus extracellular matrix protein FRAS1-like (LOC107131184), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235464
    Clone ID Related Accession (Same CDS sequence) XM_024993475.1
    Accession Version XM_024993475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: extracellular matrix protein FRAS1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGCCAGCTT ATGCTTCTTT GCAGGATGCC ACCATTTGGA AGCCCGATTC ATGCCAGAAC 
    TGCCGTTGCC ATGGTGATAT CGTTATCTGC AAACCCGCTG TTTGCAGAAA CCCTCAGTGT
    GCCTTTGAGA AGGGGGAAGT GCTTCAAATA CCCGCCAACC AGTGCTGCCC TGAATGTGTG
    CCAAGGATGC CTGGATCTTG CCATTATGAA AAGAAAATTC ACGGGCACGG GACAGCGTGG
    GCCTCCTCCC CTTGCAGCAC GTGCTCTTGC ACTCATGGGG AAGTCCGATG TGCCCCCCAA
    CCATGCCCAC CACTGATGTG TGGACACCAG GAGCTGGAAT TCATCCCCGA AGGGAGCTGC
    TGCCCAGTCT GTGTGGGCCC TGGGAAATCC TGTTCCTACG AAGGCCATGT GTTTCAAGAT
    GGGNAGGACT GGCCATTGAG TCGGTGCGCC AAGTGTGTGT GTAGAAATGG GGTCGCCCAG
    TGTTTTGCAG CTCAGTGTCC TCCTCTGTTT TGTAACCAGG ATGAAACCGT CATCCGAGTC
    CCTGGAAAGT GCTGTCCACA GTGCTCTTCG CTGTCGTGCT CTGCTGCTGG CCAAGTGTAC
    GAGCATGGAG AGCAGTGGCA AGAAAATGCC TGCACCACGT GTACATGTGA CCAGGGCCAG
    GTCAGGTGTC ACAAACAGCC CTGCCCTCCA TTAAGATGCG AAAAGGGTCA GAGACGGGCT
    CGGCTTCCAG GGCAATGCTG TGAGGACTGT GTGTCTCCTG CTGGAAGCTG CTCGCACAGA
    GGGCTCGTGC GATACCAGGA TGATATGTGG AAGGGCTCGG CCTGTGAGTT CTGCGTGTGC
    AACCGTGGCC AAGTGACCTG CCAGACTGCA GAGTGTGCCA AAGTGGAGTG TGCCCAGGGT
    GAAGAATTAA TTCACTTAGA GGGAAAATGC TGTCCTGAAT GCATTTCAAG GAATGGTTAT
    TGTGTTTACG AAGAAAATGC AGAATTTATG TCCTCAAATG CAAGTAAAGT TAAACGTATT
    CCAGAAGGAG AGAAGTGGGA GGATGGCCCT TGCAAGGTGT GTGAGTGCCG GGGGGCTCAG
    ATAACTTGCT ACGAGCCCTC TTGTCCTCCA TGCCCGGTGG CCACACTAGC CCAGGCGGTA
    AAGGGACAGT GCTGTCCCGA TTGCACATCA GTTCGTTGCC ACCCAGACTG CTTGACCTGC
    TCTCAGTCTC CAGACCACTG TGACCTCTGC CAGGATCCCA CCAAGTTGCT GAGGAATGGA
    CAATGTGTGC ACAGCTGTGG TCTGGGGTTT TACCAAGCTG GTGCTCTCTG TTTAGCCTGC
    CAGCCACAGT GCTCCACCTG CACCAGTGGG CTCGAGTGCT CGTCCTGCCA GCCTCCCCTG
    CTGATGCAGC AGGGCCAGTG TGTGTCTACC TGTGGGATCG GCTTCTACCA AGACCGCCAC
    TCCTGTGCAG CCTGCCACGA GTCCTGTGCC GCCTGCTGGG GTCCCACAGA GAAGCACTGC
    CTGGCCTGCA GAGACCCCCT CCACGTGCTA AGAGAGGGTG GCTGTGAGAG CAGCTGTGGC
    AACCGGTTTT ATAACAAGCA GGGGACCTGC AGTGCTTGTG ATCAATCCTG TAAGAGTTGT
    GGCCCTAGTA GTCCCAGATG TCTTACCTGT GTGGAGAAGA CCGTGTTGCA TGATGGGAAA
    TGCATTTCTG AATGCCCTGG TGGGTACTAT GCTGATGCCA CTGGGAGATG TAAAGTTTGT
    CACAACTCAT GTGCCAGCTG CTCAGGACCC ACGGCCGCTC ACTGCACCGC CTGCATCCAC
    CCCCAGGCCC TGCGCCAAGG CCACTGTCTG CCCAACTGTG GAGAGGCCTT CTACCCTGAC
    CATGGTGTCT GCAAAGCCTG TCATACCTCT TGCTTGACCT GTGTGGGTCC AGCACACTCC
    CACTGCACCC AGTGCAGGAA GCCAGAGGAC GGACTGCAGT TTGAGCCGCT GTCAGGCGCA
    AACATCACCT CAGGCGAGTG TCTGTCCCGG TGCAGAGCCC AGTTTTACTT GGAGATCACT
    GGACTGTGTG AAGCTTGCCA CCCATCCTGT TCTGCGTGTG CAGGGATGAG CCCACACAAC
    TGCACAGCCT GTTGGCCTTC CCACATGCTG TTGGATGGGG AGTGCCTCTC CCAGTGCCCA
    GATGGGTACT TCAACCAGGA AGGCAGTTGT ACAGAGTGCC ACCCCACCTG CAGGCAATGC
    CACGGCCCGT TGGAGTCTGA CTGCATCTCC TGCCACCCTC ACGTTCCTCT GACTGCTGGG
    AGCTGCAGGG CCCGCTGTAA AGAGGAGCAG TTCCTCAACC TCGTGGGCTA CTGTGCTGAC
    TGCCACCCTC TGTGCCATCA CTGTGCAGCC AACCTCCGTG ACACCGGGAG CGTCTGCCTG
    CGGTGCCAGA ATGCCCGGAA CCTGCTGCTG GGGGACCGCT GTGTCCCTGA TTGCCCTTCT
    GGGTACTTCG TGGAGAGAGG AGCTTGTAAA AAATGTCACT CCTCCTGCAG GACCTGCCAG
    GGCAGGGGTC CGTTCTCCTG CTCCTCCTGT GACACTGGCC GTGTTCTGTC CCACCTTGGC
    ACCTGCAGTA TCGCCTGCTT CCCCGGGCAC TATCTCGATG ATAACCGTGT TTGTCAGCCA
    TGCAACATGC ATTGTGGACG CTGTGATTCA CAGGCCAGCT GTACCTCCTG CCGAGATCCA
    AGCAAGGTCC TGCTCTTTGG GGACTGTCAG CATGAGAGCT GTGCCCCGCA ATACTATCTG
    GACTTCTCCA CCAAAACGTG CAAAGAGTGT GACTGGAGTT GCAATGTGTG CAGCGGGCCT
    CTTAGGACAG ACTGCCTCCA ATGCATGGAC GGCTATGTTC TCCAGGACGG CGCCTGCGTG
    GAGCAGTGCT CAGCGTCATT TTACCGGGAC CTGGGCCTCT GCAAGAGCTG TGAGAGCCAC
    TGTCTCCAAT GCCAAGGTCC CCATGAGTGC ACCCGCTGTG AAGAGCCGTT TCTCCTCTTG
    GAAGCCCAGT GTGTCCAGGA ATGCGGGAAG GGTTTCTTTG CAGACCACGC TAACCACAAA
    TGCACAGCCT GCCCTAAGGG GTGCTTGCAG TGCAGCCGCA GAGACCGGTG TCACCTCTGT
    GACCAGGGCT TCTTTCTCAA GAGCGGTCTT TGTCTTTCCA ACTGTGTTCC TGGCTTCTCC
    GCCCACTCTT CTAATGAGAC ATGTGCTGGC AAAATACACA CCCCTAGTCT TCACGTGAAT
    GGTTCCCTCA CCCTTGCAAT TGGTTCAATG AAGCCACTGG ATTTTTTCCT CCTGAATGTT
    CAAGACCAGG GTGGCAGGGT TGAAGACCTA CTGTTCCATG TGGTGAGCGC CCCCACCAAT
    GGTCAGCTCG TGCTCTCAAG GAACGGAAAA GAGGCTCAGC TCGACAAGGC TGGCCACTTC
    AGTTGGAGAG ACGTGAAGGA GAAAAAAGTG CGTTTTGTGC ACAGCAGAGA AAAGCCCAGG
    AAAGGTTACT TTTCTCTGAA AATTAGTGAC CAGCAGTTCT TCTCTGAGCC ACAGCTGATC
    AACATACAAG CATTTTCAAC ACAGGCCCCC TATGTTCTGA GGAATGAGGT CCTCCATATC
    AACAGAGGAG AGAGGGGAAC CATCACCACC CAGCTGCTTG ACATCAGAGA TGATGACAAC
    CCACAGGACG TGGTGGTCGA GGTGCTCGAT CCTCCACTTC ACGGCCAGTT GCTCCAAACG
    CTCCAGTCCC CGGCGGCCCC TATCTATCAG TTTCGTCTGG ACGAGCTCTC TAGGGGCCTT
    GTCCAGTATG TTCACGATGG CTCGGATAGC ACTTCTGACA TCACAGTCTT GCAGGCCAAT
    GATGGACACT CCTTCCAAAA TATACTGTTT CATGTGAGGA CCGTGCCCAA GAATGATCGA
    GGTCTTCGGC TTGTGGCTAA TTCAATGGTA TGGGTTCCAG AAGGCGGGAT GCTGCAGATT
    ACTAACAGAA TCTTACAGGC AGAAGCTCCA GGTGCCAGTG CCTCAGAAAT CATCTACAAA
    ATCACACAGG ACCACCCCCA ATTTGGGGAG GTGGTCCTTC TGGTTAATAT GCCTGCAGAC
    AGCCCTGCAG ATGGAGGGCA GCACCTGCCT GATGGGAGGA CAGCAACTCC CACCAGCACC
    TTCACCCAGC AAGATATCAA CGAAGGCATC GTGTGGTACA GGCACTCAGG GGTCCCAGCC
    CAGAGTGACT CCTTCCGCTT TCAGGTGTCC AGCGCCACAG AGGCCCACAC CCATCTGGAG
    AGTCGCCTGT TCAACATTGC CATCTTACCT CACGCTCTGG AAACACCCCA ACACTTTCCA
    GGATCTCTCC ACATGACAGC TCGGGAAGAT GGCCTGACTG TGATTCAGCC TCATTCTCTC
    TCCTTTGTAA ACTCTGAGAG GCCAAACAGA AAGCTAATAT ACAACATCAC TCTACCGCTG
    CATCCAAATC AAGGCATCAT CGAACACAGG GACCAGCCTC ACTCTCCCAT CCGGTATTTC
    TCCCAGGAAG ACGTTAACCA GGGCAACATC ATGTACCGTC CTCCCACTGC AGCCCCCCAC
    CTCCAGGAGA TCAGGGCCTT CTCCTTTGCT GGTCTCCCAG AATCGGTGAA ATTCCACTTC
    ACAGTTTCTG ATGGAGAGCA CACGAGTCCA GAGATGGCCC TCACCATTCA CTTACTTCGC
    ACAGATCAGT ACCCACCAGT GTTCCAGGTC ACAGCTCCTC TGCTTGTGGT CAGCCCAGGA
    GGCAGCACTT CTATAGAACT TCAGCTGGTA GTACGAGACA TGGAAACAGT GCCTGAAGAA
    CTCCTCTTTG AGCTTCGGAA GCCTCCACAG CATGGCGTGC TTATTAAGTA CACAGCTGGG
    TCACCAGGGC CCATGGCCAC AGGTGACACT TTCACATATG AGGATGTAGA GAAAAATGTC
    CTGCGGTATC TACACGATGG CTCATCTGCC CGGGAGGACA GCATGGAGAT CTCAGTCACA
    GACGGCCTCT CAGCCACAAC AGTGGACGTG AGGGTGGAGG TATCACCGGC TGAGGCCCAG
    GGGCCCCGAC TGGCTGCTGG TGCCTCGCTG AGCGTCACGG TTGCCAGTAA AAGCACAGCT
    GTGATCACTC GGTCACACCT GGCTTACGTG GATGATTCTT CCTCCGACCC AGAGATCTGG
    ATTCAGATAA CTTCTCTGCC CATGTACGGT GCTCTCTTAA AAACCGCAGG ACCTGAGGTG
    GAAGAACTCT CAGAGATTTC TAATTTCACA ATGGAAGACA TCAACAACAA GAAAATTAGG
    TATTCAGCCG CGTTTGAGAC CGGTGGTCAT CCAGTTACTG ACAGCTTCCA CTTCTCTGTC
    TCTGATATGG ACCACAACCG TCTGGACAAT CAGAAGTTTA CCATCACCAT CACACCCGTC
    AAGGATCCAC CCCCAGTCAT TGCTTTTGCT GACCTTATCA CGGTTGACGA GGGAGGGAGG
    ACCCCACTCT CTTTTCACCA TTTCTTCGCT GCTGCTGCTG AGGACAGTTT CCAGGAGGAT
    GCCATCATTA AGCTAAGCGC CCTGCCCCAG TACGGCTGCA TCGAGAACAC GGGGACAGGT
    GATCGGTTTG GCCCTGGAAC TACCAGTGAC CTAGAGGCAT CGTTCCCTAT TCAGGATGTC
    CTGGAAAACT ACATTTACTA CTTTCAGAGT GTTCATGAAA GCATTGAACC AACCCACGAT
    ATTTTCAGTT TTTATGTAAG TGATGGAAAC AGTCGATCAG AAGTCCACAG CATCAACATC
    ACCATTGAGA GGAAGAATGA CGAGCCTCCC AGAATGACCT TGAGGCCCCT TGGCGTGCAG
    CTGAGCTCAG GAGTGGTGAT AAGCAATTCT TCTTTGAGCC TGCAAGACCT GGACACTCCA
    GATAATGAGC TGATTTTTGT ATTGACAAAA AAGCCTGGCC ACGGTCATCT GCTCTGGAGG
    CAAACTGCTT CTGAACCTCT GGAGAATGGG AGGGTTTTGG CCCAGGGCTC CTCCTTCACC
    TACCAAGATA TCCTGGCTGG GCTAGTTGGG TACATGCCTG GTGGTCCAGG TGTGGCAGTG
    GATGAGTTCC AGTTCTCCCT CACTGATGGC CTCCACACAG ACACGGGGAG GATGGAGATA
    TATATAGAAC TGCCTACAAG TGACATCCCC AACCTGGCTG TAAACAGAGG CCTCCAGGTC
    TCAGCAGGGT CCGTAGCAAC CATCACAGAT TGGCACTTGA AAGTGACGGC TCCTGACTCA
    GATGACCACC AGGTGATGTA CATCTTAAAG GAAGACCCTG GAGTAGGGCG CCTGCAGATG
    GTAAAGCGTG ACAGCCTGGA GCAGATCTCC ACTAGAGGCC CCATCCAAAG TTTCACCCAG
    GCTGACATCA GTCAAGGCCT TATAGAATAC AGTCATGGAA AAGGAGAATC TGGTGGGAAC
    TTTGCTTTTA AATTTGATGT GGTTGATGGA GAAGGCAACA AACTGATCGG CCAGTCATTT
    TCTATCAGTG TTTCAGAAGA CAAATCCCCA CCAGTCATCA TCACCAATAA AGGGCTGTTC
    TTGGATGAAA ACTCGGTGAA AAAGATCACC ACTCTGCAAC TCTCTGCCAC TGACCAGGAG
    GCGGAACCCA CAGAACTGGT CTACCGAATC ATCAGAGAGC CCCAGCTGGG CCGCCTGGAG
    CACACAGCAT CACCAGGCAT CCAGATTAGT TCCTTTACGC AAGCTGACCT CACTTCAGGA
    AACGTTCAGT ATATCCACTC CAGTGAGACT GAGAAGCATT CAGATGCCTT CAGCTTTACG
    CTCTCTGATG GAGTCCACGA GGTGACTCAG ACTTTCCGCA TCACTCTTCG CCCTGTGGAT
    GACTCGCTGC CCCACGTGCA AAACTTTGGG ATGCGGGTAC AGGAGGGTGT GAGGAAGACC
    ATCACGGAGT TTGAGCTTAA AGCGGTGGAT GAAGACACAG AGGCCGAGTC TGTCACGTTC
    ACGGTCGTGC AGCCCCCACG CCACGGCACC ATCGAGCGAA CCGCCGATGG GCAGCCCTTC
    CAGCTCATGT CCACCTTCAC CATGGAGGAC ATCTACCAGA GCCGGGTCCG CTACAGCCAC
    GATGGCAGCA ACTCCCTCAG AGACCGATTC ACCTTCACCG TGGCTGATGG GACGAATCCC
    TTCTTCATTG TTGAGGAAGG AGGAAAAGAG ATCATAACAG CAGCACCTCA GCAGTTCTGG
    ATAGACATCC TTCCAGTGGA TGATGGCACC CCTAGGATTG TTACCAACCT CGGGCTCCAG
    TGGCTGGAGT ACATGGATGG CCAGGCAACC AACTTGATCA CCAAGAAGGA ACTGCTGACC
    ATGGACCCGG ACACGGAGGA CCTGCAACTT GTCTATGAGA TCACCACGGG CCCCAAGCAT
    GGCCACGTGG AGAACAAACT GCAGCCTGGC AGGGCAGTGG CTACCTTCAC ACAGGAGGAC
    GTGAACTTGG GGTTGATTCG TTACGTGTTG CATGAAGAGA AGATCCATGA GATGATGGAT
    AGTTTTCAGT TTCTGGTGAA AGATAGTAAA CCCAATGTGG TCAACGACAA CATCTTCCAT
    ATCCAGTGGT CCCTCATCAG TTTTAAATAT ACCAGCTACA ATGTCAGTGA GAAAGCAGGG
    TCAGTGAGTG TCACGGTGCA GAGGACGGGG AACCTGAACC AGTATGCCAT TGTCCTGTGT
    CGCACGGAGC AAGGCACGGC CACCTCCAGC TCCCGGGTGG GCTCCCAGCC GGGACAGCAG
    GACTATGTGG AGTATGCTGG CCAGGTGCAG TTTGATGAGC GAGAGGACAC CAAGTCCTGC
    ACCATCGTCA TCAACGATGA CAACGTGTTT GAGGACGTCG AGAGTTTCAC CGTGGAGCTC
    AGCATGCCAG CGTATGCCCT ACTAGGGGAG TTCACCCAGG CGAAGGTCAT TATCAATGAC
    ACTGAGGACG AGCCTACATT AGAGTTTGAT AAGAAGACCT ACCGGGTCAA CGAGAGTGCT
    GGCTTTCTGT TTGCACCTAT TGAAAGAAAA GGGGATTCAA GCAGCATTGT ATCTGTGATT
    TGCTACACGG TCCCCAAGTC AGCTATGGGC AGTAGCCTCT ATGCTCTAGA ATCGGGCTCT
    GATTTTAAAT CTAGAGGGAT GTCTGCCGAG AGTCGAGTGA TATTTGGGCC TGGTGTCACC
    ATGTCCACCT GTGATGTCAT GCTCATCGAT GACAGCGAGT ACGAAGAGGA AGAGGAGTTT
    GAGATTGCCC TGACAGGTGC TTCCGACAAC GCCCGCATCG GAAGAATGGC ATCGGCCAAG
    GTGCTCATTG CTGGTCCCAA TGATGCTTCT ACCGTGTCCT TGGGCAACAC GGCTTTTACC
    ATCAGTGAGG ATGCAGGTAC AGTGAAGATC CCAGTTATCC GCCACGGGAC TGACCTTTCC
    ACCTTCACGT CTGTCTGGTG TGCCACGAGG CCCTCAGACC CAGCGTCTGC CACCCCAGGA
    GTTGACTACG TTCCCAGCTC TAGGAAGGTG GAGTTCGGAC CAGGTGTCAC TGAGCAGTAT
    TGCACCTTGA CCATCTTGGA TGACACTCAG TATCCAGTGA TTGAAGGGCT GGAGACATTT
    GTGGTTTTCC TCAGCTCCCC ACAAGGGGCC GAACTGACCA AACCCTTCCA GGCTGTCATT
    GCAATTAATG ACACATTCCA GGATGTGCCC AGCATGCAGT TTGCCAAGGA TTTGCTCCTG
    GTGAAGGAGA AGGAGGGCGT CCTGCATGTG CCCATCATTC GGAGTGGAGA CCTGAGCTAT
    GAGTCATCGG TGAGGTGCTA TACTCAGGGC CTTTCAGCTC AGGTCATGGA GGACTTTGAG
    GAGAGAAGAA ACGCAGACTC TTCACGGATT ACGTTTCTCA AAGGGGAGAA AATGAAGAAC
    TGTACTGTCT ATATACACGA TGACTCCATG TTTGAGCCTG AGGAACAGTT CAGGGTCTAC
    CTTGGCCGTC CTCTTGGGAA CCACTGGAGT GGAGCCAGAG TTGGAAAGAA TCACATGGCC
    ACCATCACCA TATCTAATGA TGAAGATGCC CCTACCATTG AGTTTGAAGA AGCTGCATAC
    CAAGTCCGGG AACCCTCAGG GCCAGATGCC ACGGCCATTC TGAACGTCAA GGTGATCCGC
    AGAGGGGATC AGAACAGAAC ATCCAAGATT CGCTGTAGCA CACGGGATGG GTCAGCCCAG
    TCTGGTGTGG ATTATTACCC AAAGAGCCGA GTCTTGAAGT TCAGTCCTGG TGTGGATCAC
    ATCTTCTTTA AAGTCGAGAT CCTGTCCAAT GAAGACCGGG AGTGGCATGA ATCCTTCTCC
    CTAGTCCTTG GCCCAGATGA CCCAGTGGAA GCAGTTCTTG GCGATGTGAC CACGGCTACA
    GTGACGATTC TAGACCAGGA GGCAGCTGGG AGTCTCATCC TGCCAGCACC GCCCATTGTC
    GTTACACTTG CTGACTATGA CCATGTGGAA GAGGTTACCA AGGAGGGAGT CAAGAAATCC
    CCCTCCCCGG GCTACCCGCT GATCTGCGTC ACGCCCTGCG ACCCCCATTT CCCCAAATAC
    GCTGTCATGA AGGAGCGCTG CATTGAGGCT GGCATCAACC AGACGTCTGT GCAGTTCAGC
    TGGGAAGTGG CCGCCCCCAC TGACGGCAAC GGGGCCCGGT CTCCCTTCGA GACCATCACC
    GACAACACGC CATTCACCAG TGTCAACCAC ATGGTCCTGG ACAGCATTTA CTTCAGCCGG
    AGGTTCCATG TACGTTGTGT GGCCAAGGCT GTGGACAAGG TGGGCCACGT GGGGACCCCC
    TTGAGGAGCA ACGTTGTCAC CATCGGAACA GACAGTGCTA TCTGCCACAC TCCAGTGGTG
    GCTGGGACAG CCCGAGGCTT CCAGGCTCAG TCCTTCATCG CGACCTTGAA ATACCTGGAC
    GTCAAACATA AGGAGCATCC CAACAGAATC CACATTTCCG TGCAGATCCC ACACCAGGAT
    GGAATGCTGC CACTCATCTC CACCATGCCG CTGCACAACC TGCATTTTCT TCTATCTGAG
    TCCATCTACA GGCACCAACA CGTCTGCTCC AACCTCGTCA CCACCCGGGA CCTGAGAGGC
    ATCTCAGAGG CAGGGTTCCT GGACGACAGG GTCCATGACA GCATGGCACT GGGGCCTGGC
    TACGACCGCC CCTTCCAGTT TGACTCCAGC GTGCGGGAGC CAAAGACCAT CCAGCTCTAC
    AAACACCTGA ACCTGAAGAG CTGCGTCTGG ACGTTTGATG CCTATTATGA CATGACGGAG
    CTGATTGACG TCTGTGGGGG GTCTGTAACC GCCGACTTCC AGGTGAGAGA CTCGGCTCAG
    TCCTTCCTGA CTGTGCACGT GCCCCTCCAC GTGTCCTACA TCTATGTGAC GGCCCCCCGG
    GGCTGGGCCT CCTTGGAGCA CCACACCGAG ATGGAGTTCT CCTTCTTCTA TGACACTGTT
    CTCTGGAGAA CAGGAATCCA GACGGACAGT GTGCTCTCTG CCAGGCTTCA GATAATAAGG
    ATCTACATTC GAGAGGATGG CCGTCTGGTT ATTGAATTCA AGACCCATGC CAAATTCAGA
    GGACAGTTTG TGATGGAGCA CCACACCCTC CCTGATGTGA AATCATTCAT ACTGACTCCA
    GACCACCTCG GAGGAATTGA ATTTGACCTG CAGCTCCTAT GGAGTGCTCA GACTTTCGAT
    TCTCCTTATC AACTCTGGCG AGCTACAAGC TCTTATAACA GGAAGGATTA CTCTGGGGAG
    TACACCATCT ACCTGATCCC TTGCACAGTG CAACCCACGC AGCCATGGGC GGACCCAGGA
    GAAAAGCCTC TGGCCTGCAC CGCACACGCC CCAGAGAGAT TCCTGATACC CATCGCATTC
    CAGCAGACCA ACCGCCCTGT CCCTGTTGTG TATTCGCTCA ATACTGAATT TCAGCTCTGC
    AACAATGAGA AGGTGTTCCT AATGGACCCC AACACTTCTG ATATGTCACT GGCAGAAATG
    GATTACAAAG GAGCCTTCTC GAAAGGTCAA ATCCTCTATG GCCGAGTACT TTGGAATCCA
    GAGCAAAACC TTAATTCTGC TTACAAGCTC CAGCTGGAGA AGGTCTATCT TTGTACTGGA
    AAGGATGGCT ACGTGCCTTT CTTTGATCCC ACAGGGACCA TCTACAATGA AGGGCCACAG
    TATGGATGCA TTCAGCCAAA CAAACATCTG AAACACAGAT TCCTGCTGCT GGACCGCAGT
    CAGCCAGAGG TAACTGACAA GTACTTCCAT GACGTGCCCT TTGAGGCCCA TTTTGCTTCC
    GAGCTGCCTG ATTTCCACGT GGTCAGCAGC ATGCCAGGCG TGGATGGATT TACTCTGAAG
    GTAGACGCGC TCTATAAGGT GGAAGCAGGA CACCAGTGGT ACCTTCAGGT GATCTACATC
    ATTGGTCCTG ATACCATCTC AGGCCCCAGG GTCCAGCGCT CTCTCACGGC CCCTCTGAGG
    CGCCACCGAA GGGACCTGGC AGACCCCAGT GGCTGGCTGA GCCTTGACGA GTCCCTCATC
    TATGACAATG AAGGAGACCA AGTCAAGAAT GGCACCAATA TGAAGTCCCT GAACCTGGAG
    ATGCAAGAGC CAGTCGTAGC TGCTTCTCTC TCCCAAACCG GGGCGTCTAT CGGCAGCGCC
    CTTGCTGCCA TCATGCTCCT ACTTCTGGTG TCTTTGGTGG CTTGTTTCAT CACCAGGAAA
    TGCCAGAAAC AAAGGAAGAA GCCGCCCACG GGGGACATCC TGGAGGAATA CCCTCTGAAC
    ACCAAGGTGG ACATGCCCAA GAGAGGGGCA GACAGGGTGG AGAAGAATGT GGACAGACAG
    TACTGCACGG TGCGCAATGT GAACATACTT GGTGAAGCCG AGCGCGCGTA CGTGTTCAGA
    GGTGCGAAAG TAAAGAAATT GAATCTGGAA GTCAGAGTTC ACAACAATTT ACAGGACGGA
    ACAGAAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849243.1
    CDS1..11952
    Translation

    Target ORF information:

    RefSeq Version XM_024993475.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Fraser extracellular matrix complex subunit 1 (FRAS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGCCAGCTT ATGCTTCTTT GCAGGATGCC ACCATTTGGA AGCCCGATTC ATGCCAGAAC 
    TGCCGTTGCC ATGGTGATAT CGTTATCTGC AAACCCGCTG TTTGCAGAAA CCCTCAGTGT
    GCCTTTGAGA AGGGGGAAGT GCTTCAAATA CCCGCCAACC AGTGCTGCCC TGAATGTGTG
    CCAAGGATGC CTGGATCTTG CCATTATGAA AAGAAAATTC ACGGGCACGG GACAGCGTGG
    GCCTCCTCCC CTTGCAGCAC GTGCTCTTGC ACTCATGGGG AAGTCCGATG TGCCCCCCAA
    CCATGCCCAC CACTGATGTG TGGACACCAG GAGCTGGAAT TCATCCCCGA AGGGAGCTGC
    TGCCCAGTCT GTGTGGGCCC TGGGAAATCC TGTTCCTACG AAGGCCATGT GTTTCAAGAT
    GGGNAGGACT GGCCATTGAG TCGGTGCGCC AAGTGTGTGT GTAGAAATGG GGTCGCCCAG
    TGTTTTGCAG CTCAGTGTCC TCCTCTGTTT TGTAACCAGG ATGAAACCGT CATCCGAGTC
    CCTGGAAAGT GCTGTCCACA GTGCTCTTCG CTGTCGTGCT CTGCTGCTGG CCAAGTGTAC
    GAGCATGGAG AGCAGTGGCA AGAAAATGCC TGCACCACGT GTACATGTGA CCAGGGCCAG
    GTCAGGTGTC ACAAACAGCC CTGCCCTCCA TTAAGATGCG AAAAGGGTCA GAGACGGGCT
    CGGCTTCCAG GGCAATGCTG TGAGGACTGT GTGTCTCCTG CTGGAAGCTG CTCGCACAGA
    GGGCTCGTGC GATACCAGGA TGATATGTGG AAGGGCTCGG CCTGTGAGTT CTGCGTGTGC
    AACCGTGGCC AAGTGACCTG CCAGACTGCA GAGTGTGCCA AAGTGGAGTG TGCCCAGGGT
    GAAGAATTAA TTCACTTAGA GGGAAAATGC TGTCCTGAAT GCATTTCAAG GAATGGTTAT
    TGTGTTTACG AAGAAAATGC AGAATTTATG TCCTCAAATG CAAGTAAAGT TAAACGTATT
    CCAGAAGGAG AGAAGTGGGA GGATGGCCCT TGCAAGGTGT GTGAGTGCCG GGGGGCTCAG
    ATAACTTGCT ACGAGCCCTC TTGTCCTCCA TGCCCGGTGG CCACACTAGC CCAGGCGGTA
    AAGGGACAGT GCTGTCCCGA TTGCACATCA GTTCGTTGCC ACCCAGACTG CTTGACCTGC
    TCTCAGTCTC CAGACCACTG TGACCTCTGC CAGGATCCCA CCAAGTTGCT GAGGAATGGA
    CAATGTGTGC ACAGCTGTGG TCTGGGGTTT TACCAAGCTG GTGCTCTCTG TTTAGCCTGC
    CAGCCACAGT GCTCCACCTG CACCAGTGGG CTCGAGTGCT CGTCCTGCCA GCCTCCCCTG
    CTGATGCAGC AGGGCCAGTG TGTGTCTACC TGTGGGATCG GCTTCTACCA AGACCGCCAC
    TCCTGTGCAG CCTGCCACGA GTCCTGTGCC GCCTGCTGGG GTCCCACAGA GAAGCACTGC
    CTGGCCTGCA GAGACCCCCT CCACGTGCTA AGAGAGGGTG GCTGTGAGAG CAGCTGTGGC
    AACCGGTTTT ATAACAAGCA GGGGACCTGC AGTGCTTGTG ATCAATCCTG TAAGAGTTGT
    GGCCCTAGTA GTCCCAGATG TCTTACCTGT GTGGAGAAGA CCGTGTTGCA TGATGGGAAA
    TGCATTTCTG AATGCCCTGG TGGGTACTAT GCTGATGCCA CTGGGAGATG TAAAGTTTGT
    CACAACTCAT GTGCCAGCTG CTCAGGACCC ACGGCCGCTC ACTGCACCGC CTGCATCCAC
    CCCCAGGCCC TGCGCCAAGG CCACTGTCTG CCCAACTGTG GAGAGGCCTT CTACCCTGAC
    CATGGTGTCT GCAAAGCCTG TCATACCTCT TGCTTGACCT GTGTGGGTCC AGCACACTCC
    CACTGCACCC AGTGCAGGAA GCCAGAGGAC GGACTGCAGT TTGAGCCGCT GTCAGGCGCA
    AACATCACCT CAGGCGAGTG TCTGTCCCGG TGCAGAGCCC AGTTTTACTT GGAGATCACT
    GGACTGTGTG AAGCTTGCCA CCCATCCTGT TCTGCGTGTG CAGGGATGAG CCCACACAAC
    TGCACAGCCT GTTGGCCTTC CCACATGCTG TTGGATGGGG AGTGCCTCTC CCAGTGCCCA
    GATGGGTACT TCAACCAGGA AGGCAGTTGT ACAGAGTGCC ACCCCACCTG CAGGCAATGC
    CACGGCCCGT TGGAGTCTGA CTGCATCTCC TGCCACCCTC ACGTTCCTCT GACTGCTGGG
    AGCTGCAGGG CCCGCTGTAA AGAGGAGCAG TTCCTCAACC TCGTGGGCTA CTGTGCTGAC
    TGCCACCCTC TGTGCCATCA CTGTGCAGCC AACCTCCGTG ACACCGGGAG CGTCTGCCTG
    CGGTGCCAGA ATGCCCGGAA CCTGCTGCTG GGGGACCGCT GTGTCCCTGA TTGCCCTTCT
    GGGTACTTCG TGGAGAGAGG AGCTTGTAAA AAATGTCACT CCTCCTGCAG GACCTGCCAG
    GGCAGGGGTC CGTTCTCCTG CTCCTCCTGT GACACTGGCC GTGTTCTGTC CCACCTTGGC
    ACCTGCAGTA TCGCCTGCTT CCCCGGGCAC TATCTCGATG ATAACCGTGT TTGTCAGCCA
    TGCAACATGC ATTGTGGACG CTGTGATTCA CAGGCCAGCT GTACCTCCTG CCGAGATCCA
    AGCAAGGTCC TGCTCTTTGG GGACTGTCAG CATGAGAGCT GTGCCCCGCA ATACTATCTG
    GACTTCTCCA CCAAAACGTG CAAAGAGTGT GACTGGAGTT GCAATGTGTG CAGCGGGCCT
    CTTAGGACAG ACTGCCTCCA ATGCATGGAC GGCTATGTTC TCCAGGACGG CGCCTGCGTG
    GAGCAGTGCT CAGCGTCATT TTACCGGGAC CTGGGCCTCT GCAAGAGCTG TGAGAGCCAC
    TGTCTCCAAT GCCAAGGTCC CCATGAGTGC ACCCGCTGTG AAGAGCCGTT TCTCCTCTTG
    GAAGCCCAGT GTGTCCAGGA ATGCGGGAAG GGTTTCTTTG CAGACCACGC TAACCACAAA
    TGCACAGCCT GCCCTAAGGG GTGCTTGCAG TGCAGCCGCA GAGACCGGTG TCACCTCTGT
    GACCAGGGCT TCTTTCTCAA GAGCGGTCTT TGTCTTTCCA ACTGTGTTCC TGGCTTCTCC
    GCCCACTCTT CTAATGAGAC ATGTGCTGGC AAAATACACA CCCCTAGTCT TCACGTGAAT
    GGTTCCCTCA CCCTTGCAAT TGGTTCAATG AAGCCACTGG ATTTTTTCCT CCTGAATGTT
    CAAGACCAGG GTGGCAGGGT TGAAGACCTA CTGTTCCATG TGGTGAGCGC CCCCACCAAT
    GGTCAGCTCG TGCTCTCAAG GAACGGAAAA GAGGCTCAGC TCGACAAGGC TGGCCACTTC
    AGTTGGAGAG ACGTGAAGGA GAAAAAAGTG CGTTTTGTGC ACAGCAGAGA AAAGCCCAGG
    AAAGGTTACT TTTCTCTGAA AATTAGTGAC CAGCAGTTCT TCTCTGAGCC ACAGCTGATC
    AACATACAAG CATTTTCAAC ACAGGCCCCC TATGTTCTGA GGAATGAGGT CCTCCATATC
    AACAGAGGAG AGAGGGGAAC CATCACCACC CAGCTGCTTG ACATCAGAGA TGATGACAAC
    CCACAGGACG TGGTGGTCGA GGTGCTCGAT CCTCCACTTC ACGGCCAGTT GCTCCAAACG
    CTCCAGTCCC CGGCGGCCCC TATCTATCAG TTTCGTCTGG ACGAGCTCTC TAGGGGCCTT
    GTCCAGTATG TTCACGATGG CTCGGATAGC ACTTCTGACA TCACAGTCTT GCAGGCCAAT
    GATGGACACT CCTTCCAAAA TATACTGTTT CATGTGAGGA CCGTGCCCAA GAATGATCGA
    GGTCTTCGGC TTGTGGCTAA TTCAATGGTA TGGGTTCCAG AAGGCGGGAT GCTGCAGATT
    ACTAACAGAA TCTTACAGGC AGAAGCTCCA GGTGCCAGTG CCTCAGAAAT CATCTACAAA
    ATCACACAGG ACCACCCCCA ATTTGGGGAG GTGGTCCTTC TGGTTAATAT GCCTGCAGAC
    AGCCCTGCAG ATGGAGGGCA GCACCTGCCT GATGGGAGGA CAGCAACTCC CACCAGCACC
    TTCACCCAGC AAGATATCAA CGAAGGCATC GTGTGGTACA GGCACTCAGG GGTCCCAGCC
    CAGAGTGACT CCTTCCGCTT TCAGGTGTCC AGCGCCACAG AGGCCCACAC CCATCTGGAG
    AGTCGCCTGT TCAACATTGC CATCTTACCT CACGCTCTGG AAACACCCCA ACACTTTCCA
    GGATCTCTCC ACATGACAGC TCGGGAAGAT GGCCTGACTG TGATTCAGCC TCATTCTCTC
    TCCTTTGTAA ACTCTGAGAG GCCAAACAGA AAGCTAATAT ACAACATCAC TCTACCGCTG
    CATCCAAATC AAGGCATCAT CGAACACAGG GACCAGCCTC ACTCTCCCAT CCGGTATTTC
    TCCCAGGAAG ACGTTAACCA GGGCAACATC ATGTACCGTC CTCCCACTGC AGCCCCCCAC
    CTCCAGGAGA TCAGGGCCTT CTCCTTTGCT GGTCTCCCAG AATCGGTGAA ATTCCACTTC
    ACAGTTTCTG ATGGAGAGCA CACGAGTCCA GAGATGGCCC TCACCATTCA CTTACTTCGC
    ACAGATCAGT ACCCACCAGT GTTCCAGGTC ACAGCTCCTC TGCTTGTGGT CAGCCCAGGA
    GGCAGCACTT CTATAGAACT TCAGCTGGTA GTACGAGACA TGGAAACAGT GCCTGAAGAA
    CTCCTCTTTG AGCTTCGGAA GCCTCCACAG CATGGCGTGC TTATTAAGTA CACAGCTGGG
    TCACCAGGGC CCATGGCCAC AGGTGACACT TTCACATATG AGGATGTAGA GAAAAATGTC
    CTGCGGTATC TACACGATGG CTCATCTGCC CGGGAGGACA GCATGGAGAT CTCAGTCACA
    GACGGCCTCT CAGCCACAAC AGTGGACGTG AGGGTGGAGG TATCACCGGC TGAGGCCCAG
    GGGCCCCGAC TGGCTGCTGG TGCCTCGCTG AGCGTCACGG TTGCCAGTAA AAGCACAGCT
    GTGATCACTC GGTCACACCT GGCTTACGTG GATGATTCTT CCTCCGACCC AGAGATCTGG
    ATTCAGATAA CTTCTCTGCC CATGTACGGT GCTCTCTTAA AAACCGCAGG ACCTGAGGTG
    GAAGAACTCT CAGAGATTTC TAATTTCACA ATGGAAGACA TCAACAACAA GAAAATTAGG
    TATTCAGCCG CGTTTGAGAC CGGTGGTCAT CCAGTTACTG ACAGCTTCCA CTTCTCTGTC
    TCTGATATGG ACCACAACCG TCTGGACAAT CAGAAGTTTA CCATCACCAT CACACCCGTC
    AAGGATCCAC CCCCAGTCAT TGCTTTTGCT GACCTTATCA CGGTTGACGA GGGAGGGAGG
    ACCCCACTCT CTTTTCACCA TTTCTTCGCT GCTGCTGCTG AGGACAGTTT CCAGGAGGAT
    GCCATCATTA AGCTAAGCGC CCTGCCCCAG TACGGCTGCA TCGAGAACAC GGGGACAGGT
    GATCGGTTTG GCCCTGGAAC TACCAGTGAC CTAGAGGCAT CGTTCCCTAT TCAGGATGTC
    CTGGAAAACT ACATTTACTA CTTTCAGAGT GTTCATGAAA GCATTGAACC AACCCACGAT
    ATTTTCAGTT TTTATGTAAG TGATGGAAAC AGTCGATCAG AAGTCCACAG CATCAACATC
    ACCATTGAGA GGAAGAATGA CGAGCCTCCC AGAATGACCT TGAGGCCCCT TGGCGTGCAG
    CTGAGCTCAG GAGTGGTGAT AAGCAATTCT TCTTTGAGCC TGCAAGACCT GGACACTCCA
    GATAATGAGC TGATTTTTGT ATTGACAAAA AAGCCTGGCC ACGGTCATCT GCTCTGGAGG
    CAAACTGCTT CTGAACCTCT GGAGAATGGG AGGGTTTTGG CCCAGGGCTC CTCCTTCACC
    TACCAAGATA TCCTGGCTGG GCTAGTTGGG TACATGCCTG GTGGTCCAGG TGTGGCAGTG
    GATGAGTTCC AGTTCTCCCT CACTGATGGC CTCCACACAG ACACGGGGAG GATGGAGATA
    TATATAGAAC TGCCTACAAG TGACATCCCC AACCTGGCTG TAAACAGAGG CCTCCAGGTC
    TCAGCAGGGT CCGTAGCAAC CATCACAGAT TGGCACTTGA AAGTGACGGC TCCTGACTCA
    GATGACCACC AGGTGATGTA CATCTTAAAG GAAGACCCTG GAGTAGGGCG CCTGCAGATG
    GTAAAGCGTG ACAGCCTGGA GCAGATCTCC ACTAGAGGCC CCATCCAAAG TTTCACCCAG
    GCTGACATCA GTCAAGGCCT TATAGAATAC AGTCATGGAA AAGGAGAATC TGGTGGGAAC
    TTTGCTTTTA AATTTGATGT GGTTGATGGA GAAGGCAACA AACTGATCGG CCAGTCATTT
    TCTATCAGTG TTTCAGAAGA CAAATCCCCA CCAGTCATCA TCACCAATAA AGGGCTGTTC
    TTGGATGAAA ACTCGGTGAA AAAGATCACC ACTCTGCAAC TCTCTGCCAC TGACCAGGAG
    GCGGAACCCA CAGAACTGGT CTACCGAATC ATCAGAGAGC CCCAGCTGGG CCGCCTGGAG
    CACACAGCAT CACCAGGCAT CCAGATTAGT TCCTTTACGC AAGCTGACCT CACTTCAGGA
    AACGTTCAGT ATATCCACTC CAGTGAGACT GAGAAGCATT CAGATGCCTT CAGCTTTACG
    CTCTCTGATG GAGTCCACGA GGTGACTCAG ACTTTCCGCA TCACTCTTCG CCCTGTGGAT
    GACTCGCTGC CCCACGTGCA AAACTTTGGG ATGCGGGTAC AGGAGGGTGT GAGGAAGACC
    ATCACGGAGT TTGAGCTTAA AGCGGTGGAT GAAGACACAG AGGCCGAGTC TGTCACGTTC
    ACGGTCGTGC AGCCCCCACG CCACGGCACC ATCGAGCGAA CCGCCGATGG GCAGCCCTTC
    CAGCTCATGT CCACCTTCAC CATGGAGGAC ATCTACCAGA GCCGGGTCCG CTACAGCCAC
    GATGGCAGCA ACTCCCTCAG AGACCGATTC ACCTTCACCG TGGCTGATGG GACGAATCCC
    TTCTTCATTG TTGAGGAAGG AGGAAAAGAG ATCATAACAG CAGCACCTCA GCAGTTCTGG
    ATAGACATCC TTCCAGTGGA TGATGGCACC CCTAGGATTG TTACCAACCT CGGGCTCCAG
    TGGCTGGAGT ACATGGATGG CCAGGCAACC AACTTGATCA CCAAGAAGGA ACTGCTGACC
    ATGGACCCGG ACACGGAGGA CCTGCAACTT GTCTATGAGA TCACCACGGG CCCCAAGCAT
    GGCCACGTGG AGAACAAACT GCAGCCTGGC AGGGCAGTGG CTACCTTCAC ACAGGAGGAC
    GTGAACTTGG GGTTGATTCG TTACGTGTTG CATGAAGAGA AGATCCATGA GATGATGGAT
    AGTTTTCAGT TTCTGGTGAA AGATAGTAAA CCCAATGTGG TCAACGACAA CATCTTCCAT
    ATCCAGTGGT CCCTCATCAG TTTTAAATAT ACCAGCTACA ATGTCAGTGA GAAAGCAGGG
    TCAGTGAGTG TCACGGTGCA GAGGACGGGG AACCTGAACC AGTATGCCAT TGTCCTGTGT
    CGCACGGAGC AAGGCACGGC CACCTCCAGC TCCCGGGTGG GCTCCCAGCC GGGACAGCAG
    GACTATGTGG AGTATGCTGG CCAGGTGCAG TTTGATGAGC GAGAGGACAC CAAGTCCTGC
    ACCATCGTCA TCAACGATGA CAACGTGTTT GAGGACGTCG AGAGTTTCAC CGTGGAGCTC
    AGCATGCCAG CGTATGCCCT ACTAGGGGAG TTCACCCAGG CGAAGGTCAT TATCAATGAC
    ACTGAGGACG AGCCTACATT AGAGTTTGAT AAGAAGACCT ACCGGGTCAA CGAGAGTGCT
    GGCTTTCTGT TTGCACCTAT TGAAAGAAAA GGGGATTCAA GCAGCATTGT ATCTGTGATT
    TGCTACACGG TCCCCAAGTC AGCTATGGGC AGTAGCCTCT ATGCTCTAGA ATCGGGCTCT
    GATTTTAAAT CTAGAGGGAT GTCTGCCGAG AGTCGAGTGA TATTTGGGCC TGGTGTCACC
    ATGTCCACCT GTGATGTCAT GCTCATCGAT GACAGCGAGT ACGAAGAGGA AGAGGAGTTT
    GAGATTGCCC TGACAGGTGC TTCCGACAAC GCCCGCATCG GAAGAATGGC ATCGGCCAAG
    GTGCTCATTG CTGGTCCCAA TGATGCTTCT ACCGTGTCCT TGGGCAACAC GGCTTTTACC
    ATCAGTGAGG ATGCAGGTAC AGTGAAGATC CCAGTTATCC GCCACGGGAC TGACCTTTCC
    ACCTTCACGT CTGTCTGGTG TGCCACGAGG CCCTCAGACC CAGCGTCTGC CACCCCAGGA
    GTTGACTACG TTCCCAGCTC TAGGAAGGTG GAGTTCGGAC CAGGTGTCAC TGAGCAGTAT
    TGCACCTTGA CCATCTTGGA TGACACTCAG TATCCAGTGA TTGAAGGGCT GGAGACATTT
    GTGGTTTTCC TCAGCTCCCC ACAAGGGGCC GAACTGACCA AACCCTTCCA GGCTGTCATT
    GCAATTAATG ACACATTCCA GGATGTGCCC AGCATGCAGT TTGCCAAGGA TTTGCTCCTG
    GTGAAGGAGA AGGAGGGCGT CCTGCATGTG CCCATCATTC GGAGTGGAGA CCTGAGCTAT
    GAGTCATCGG TGAGGTGCTA TACTCAGGGC CTTTCAGCTC AGGTCATGGA GGACTTTGAG
    GAGAGAAGAA ACGCAGACTC TTCACGGATT ACGTTTCTCA AAGGGGAGAA AATGAAGAAC
    TGTACTGTCT ATATACACGA TGACTCCATG TTTGAGCCTG AGGAACAGTT CAGGGTCTAC
    CTTGGCCGTC CTCTTGGGAA CCACTGGAGT GGAGCCAGAG TTGGAAAGAA TCACATGGCC
    ACCATCACCA TATCTAATGA TGAAGATGCC CCTACCATTG AGTTTGAAGA AGCTGCATAC
    CAAGTCCGGG AACCCTCAGG GCCAGATGCC ACGGCCATTC TGAACGTCAA GGTGATCCGC
    AGAGGGGATC AGAACAGAAC ATCCAAGATT CGCTGTAGCA CACGGGATGG GTCAGCCCAG
    TCTGGTGTGG ATTATTACCC AAAGAGCCGA GTCTTGAAGT TCAGTCCTGG TGTGGATCAC
    ATCTTCTTTA AAGTCGAGAT CCTGTCCAAT GAAGACCGGG AGTGGCATGA ATCCTTCTCC
    CTAGTCCTTG GCCCAGATGA CCCAGTGGAA GCAGTTCTTG GCGATGTGAC CACGGCTACA
    GTGACGATTC TAGACCAGGA GGCAGCTGGG AGTCTCATCC TGCCAGCACC GCCCATTGTC
    GTTACACTTG CTGACTATGA CCATGTGGAA GAGGTTACCA AGGAGGGAGT CAAGAAATCC
    CCCTCCCCGG GCTACCCGCT GATCTGCGTC ACGCCCTGCG ACCCCCATTT CCCCAAATAC
    GCTGTCATGA AGGAGCGCTG CATTGAGGCT GGCATCAACC AGACGTCTGT GCAGTTCAGC
    TGGGAAGTGG CCGCCCCCAC TGACGGCAAC GGGGCCCGGT CTCCCTTCGA GACCATCACC
    GACAACACGC CATTCACCAG TGTCAACCAC ATGGTCCTGG ACAGCATTTA CTTCAGCCGG
    AGGTTCCATG TACGTTGTGT GGCCAAGGCT GTGGACAAGG TGGGCCACGT GGGGACCCCC
    TTGAGGAGCA ACGTTGTCAC CATCGGAACA GACAGTGCTA TCTGCCACAC TCCAGTGGTG
    GCTGGGACAG CCCGAGGCTT CCAGGCTCAG TCCTTCATCG CGACCTTGAA ATACCTGGAC
    GTCAAACATA AGGAGCATCC CAACAGAATC CACATTTCCG TGCAGATCCC ACACCAGGAT
    GGAATGCTGC CACTCATCTC CACCATGCCG CTGCACAACC TGCATTTTCT TCTATCTGAG
    TCCATCTACA GGCACCAACA CGTCTGCTCC AACCTCGTCA CCACCCGGGA CCTGAGAGGC
    ATCTCAGAGG CAGGGTTCCT GGACGACAGG GTCCATGACA GCATGGCACT GGGGCCTGGC
    TACGACCGCC CCTTCCAGTT TGACTCCAGC GTGCGGGAGC CAAAGACCAT CCAGCTCTAC
    AAACACCTGA ACCTGAAGAG CTGCGTCTGG ACGTTTGATG CCTATTATGA CATGACGGAG
    CTGATTGACG TCTGTGGGGG GTCTGTAACC GCCGACTTCC AGGTGAGAGA CTCGGCTCAG
    TCCTTCCTGA CTGTGCACGT GCCCCTCCAC GTGTCCTACA TCTATGTGAC GGCCCCCCGG
    GGCTGGGCCT CCTTGGAGCA CCACACCGAG ATGGAGTTCT CCTTCTTCTA TGACACTGTT
    CTCTGGAGAA CAGGAATCCA GACGGACAGT GTGCTCTCTG CCAGGCTTCA GATAATAAGG
    ATCTACATTC GAGAGGATGG CCGTCTGGTT ATTGAATTCA AGACCCATGC CAAATTCAGA
    GGACAGTTTG TGATGGAGCA CCACACCCTC CCTGATGTGA AATCATTCAT ACTGACTCCA
    GACCACCTCG GAGGAATTGA ATTTGACCTG CAGCTCCTAT GGAGTGCTCA GACTTTCGAT
    TCTCCTTATC AACTCTGGCG AGCTACAAGC TCTTATAACA GGAAGGATTA CTCTGGGGAG
    TACACCATCT ACCTGATCCC TTGCACAGTG CAACCCACGC AGCCATGGGC GGACCCAGGA
    GAAAAGCCTC TGGCCTGCAC CGCACACGCC CCAGAGAGAT TCCTGATACC CATCGCATTC
    CAGCAGACCA ACCGCCCTGT CCCTGTTGTG TATTCGCTCA ATACTGAATT TCAGCTCTGC
    AACAATGAGA AGGTGTTCCT AATGGACCCC AACACTTCTG ATATGTCACT GGCAGAAATG
    GATTACAAAG GAGCCTTCTC GAAAGGTCAA ATCCTCTATG GCCGAGTACT TTGGAATCCA
    GAGCAAAACC TTAATTCTGC TTACAAGCTC CAGCTGGAGA AGGTCTATCT TTGTACTGGA
    AAGGATGGCT ACGTGCCTTT CTTTGATCCC ACAGGGACCA TCTACAATGA AGGGCCACAG
    TATGGATGCA TTCAGCCAAA CAAACATCTG AAACACAGAT TCCTGCTGCT GGACCGCAGT
    CAGCCAGAGG TAACTGACAA GTACTTCCAT GACGTGCCCT TTGAGGCCCA TTTTGCTTCC
    GAGCTGCCTG ATTTCCACGT GGTCAGCAGC ATGCCAGGCG TGGATGGATT TACTCTGAAG
    GTAGACGCGC TCTATAAGGT GGAAGCAGGA CACCAGTGGT ACCTTCAGGT GATCTACATC
    ATTGGTCCTG ATACCATCTC AGGCCCCAGG GTCCAGCGCT CTCTCACGGC CCCTCTGAGG
    CGCCACCGAA GGGACCTGGC AGACCCCAGT GGCTGGCTGA GCCTTGACGA GTCCCTCATC
    TATGACAATG AAGGAGACCA AGTCAAGAAT GGCACCAATA TGAAGTCCCT GAACCTGGAG
    ATGCAAGAGC CAGTCGTAGC TGCTTCTCTC TCCCAAACCG GGGCGTCTAT CGGCAGCGCC
    CTTGCTGCCA TCATGCTCCT ACTTCTGGTG TCTTTGGTGG CTTGTTTCAT CACCAGGAAA
    TGCCAGAAAC AAAGGAAGAA GCCGCCCACG GGGGACATCC TGGAGGAATA CCCTCTGAAC
    ACCAAGGTGG ACATGCCCAA GAGAGGGGCA GACAGGGTGG AGAAGAATGT GGACAGACAG
    TACTGCACGG TGCGCAATGT GAACATACTT GGTGAAGCCG AGCGCGCGTA CGTGTTCAGA
    GGTGCGAAAG TAAAGAAATT GAATCTGGAA GTCAGAGTTC ACAACAATTT ACAGGACGGA
    ACAGAAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90112
    Clone ID Related Accession (Same CDS sequence) XM_015463814.1
    Accession Version XM_015463814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: extracellular matrix protein FRAS1-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 4 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    ATGAGTGTCT TCCAAGTGTG GCTCGGGATG GCTCTCACAT TGGCGGAATT TGCAGCATTG 
    CCTCATCATT CTGAAGGTGC TTGTGTCTAT CAGGGTTCCT TGTTGGCGGA TGCCACCATT
    TGGAAGCCCG ATTCATGCCA GAACTGCCGT TGCCATGGTG ATATCGTTAT CTGCAAACCC
    GCTGTTTGCA GAAACCCTCA GTGTGCCTTT GAGAAGGGGG AAGTGCTTCA AATACCCGCC
    AACCAGTGCT GCCCTGAATG TGTGCCAAGG ATGCCTGGAT CTTGCCATTA TGAAAAGAAA
    ATTCACGGGC ACGGGACAGC GTGGGCCTCC TCCCCTTGCA GCACGTGCTC TTGCACTCAC
    GGGGAAGTCC GATGTGCCCC CCAACCATGC CCACCACTGA TGTGTGGACA CCAGGAGCTG
    GAATTCATCC CCGAAGGGAG CTGCTGCCCA GTCTGTGTGG GCCCTGGGAA ATCCTGTTCC
    TATGAAGGCC GTGTGTTTCA AGATGGAGAG GACTGGCCAT TGAGTCGGTG CGCCAAGTGT
    GTGTGTAGAA ATGGGGTTGC CCAGTGTTTT GCAGCTCAGT GTCCTCCTCT GTTTTGTAAC
    CAGGATGAAA CCGTCATCCG AGTCCCTGGA AAGTGCTGTC CACAGTGCTC TTCGCTGTCG
    TGCTCTGCTG CTGGCCAAGT GTACGAGCAT GGAGAGCAGT GGCAAGAAAA TGCCTGCACC
    ACGTGTACAT GTGACCAGGG CCAGGTCAGG TGTCACAAAC AGCCCTGCCC TCCATTAAGA
    TGCGAAAAGG GTCAGAGACG GGCTCGGCTT CCAGGGCAAT GCTGTGAGGA CTGTGTGTCT
    CCTGCTGGAA GCTGCTCGCA CAGAGGGCTT GTGCGATACC AGGATGATAT GTGGAAGGGC
    TCGGCCTGTG AGTTCTGCGT GTGCAACCGT GGCCAAGTGA CCTGCCAGAC TGCAGAGTGT
    GCCAAAGTGG AGTGTGCCCA GGGTGAAGAA TTAATTCACT TAGAGGGAAA ATGCTGTCCT
    GAATGCATTT CAAGGAATGG TTATTGTGTT TACGAAGAAA ATGCAGAATT TATGTCCTCA
    AATGCAAGTA AAGTTAAACG TATTCCAGAA GGAGAGAAGT GGGAGGATGG CCCTTGCAAG
    GTGTGTGAGT GCCGGGGGGC TCAGATAACT TGCTACGAGC CCTCTTGTCC TCCATGCCCG
    GTGGCCACAC TAGCCCAGGC GGTAAAGGGA CAGTGCTGTC CCGATTGCAC ATCAGTTCGT
    TGCCACCCAG ACTGCTTGAC CTGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAGGAT
    CCCACCAAGT TGCTGAGGAA TGGACAATGT GTGCACAGCT GTGGTCTGGG GTTTTACCAA
    GCTGGTGCTC TCTGTTTAGC CTGCCAGCCA CAGTGCTCCA CCTGCACCAG TGGGCTCGAG
    TGCTCGTCCT GCCAGCCTCC CCTGCTGATG CAGCAGGGCC AGTGTGTGTC TACCTGTGGG
    ATCGGCTTCT ACCAAGACCG CCACTCCTGT GCAGCCTGCC ACGAGTCCTG TGCCGCCTGC
    TGGGGTCCCA CAGAGAAGCA CTGCCTGGCC TGCAGAGACC CCCTCCACGT GCTAAGAGAG
    GGTGGCTGTG AGAGCAGCTG TGGCAACCGG TTTTATAACA AGCAGGGGAC CTGCAGTGCT
    TGTGATCAAT CCTGTAAGAG TTGTGGCCCT AGTAGTCCCA GATGTCTTAC CTGTGTGGAG
    AAGACCGTGT TGCATGATGG GAAATGCATT TCTGAATGCC CTGGTGGGTA CTATGCTGAT
    GCCACTGGGA GATGTAAAGT TTGTCACAAC TCATGTGCCA GCTGCTCAGG ACCCACGGCC
    GCTCACTGCA CCGCCTGCAT CCACCCCCAG GCCCTGCGCC AAGGCCACTG TCTGCCCAAC
    TGTGGAGAGG CCTTCTACCC TGACCATGGT GTCTGCAAAG CCTGTCATAC CTCTTGCTTG
    ACCTGTGTGG GTCCAGCACA CTCCCACTGC ACCCAGTGCA GGAAGCCAGA GGACGGACTG
    CAGTTTGAGC CGCTGTCAGG CGCAAACATC ACCTCAGGCG AGTGTCTGTC CCGGTGCAGA
    GCCCAGTTTT ACTTGGAGAT CACTGGACTG TGTGAAGCTT GCCACCCATC CTGTTCTGCG
    TGTGCAGGGA TGAGCCCACA CAACTGCACA GCCTGTTGGC CTTCCCACAT GCTGTTGGAT
    GGGGAGTGCC TCTCCCAGTG CCCAGATGGG TACTTCAACC AGGAAGGCAG TTGTACAGAG
    TGCCACCCCA CCTGCAGGCA ATGCCACGGC CCGTTGGAGT CTGACTGCAT CTCCTGCCAC
    CCTCACGTTC CTCTGACTGC TGGGAGCTGC AGGGCCCGCT GTAAAGAGGA GCAGTTCCTC
    AACCTCGTGG GCTACTGTGC TGACTGCCAC CCTCTGTGCC ATCACTGTGC AGCCAACCTC
    CGTGACACCG GGAGCGTCTG CCTGCAGTGC CAGAATGCCC GGAACCTGCT GCTGGGGGAC
    CGCTGTGTCC CTGATTGCCC TTCTGGGTAC TTCGTGGAGA GAGGAGCTTG TAAAAAATGT
    CACTCCTCCT GCAGGACCTG CCAGGGCAGG GGTCCGTTCT CCTGCTCCTC CTGTGACACT
    GGCCGTGTTC TGTCCCACCT TGGCACCTGC AGTATCGCCT GCTTCCCCGG GCACTATCTC
    GATGATAACC GTGTTTGTCA GCCATGCAAC ATGCATTGTG GACGCTGTGA TTCACAGGCC
    AGCTGTACCT CCTGCCGAGA TCCAAGCAAG GTCCTGCTCT TTGGGGACTG TCAGCATGAG
    AGCTGTGCCC CGCAATACTA TCTGGACTTC TCCACCAAAA CGTGCAAAGA GTGTGACTGG
    AGTTGCAATG TGTGCAGCGG GCCTCTTAGG ACAGACTGCC TCCAATGCAT GGACGGCTAT
    GTTCTCCAGG ACGGCGCCTG CGTGGAGCAG TGCTCAGCGT CATTTTACCG GGACCTGGGC
    CTCTGCAAGA GCTGTGAGAG CCACTGTCTC CAATGCCAAG GTCCCCATGA GTGCACCCGC
    TGTGAAGAGC CGTTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGCGG GAAGGGTTTC
    TTTGCAGACC ACGCTAACCA CAAATGCACA GCCTGCCCTA AGGGGTGCTT GCAGTGCAGC
    CGCAGAGACC GGTGTCACCT CTGTGACCAG GGCTTCTTTC TCAAGAGCGG TCTTTGTCTT
    TCCAACTGTG TTCCTGGCTT CTCCGCCCAC TCTTCTAATG AGACATGTGC TGGCAAAATA
    CACACCCCTA GTCTTCACGT GAATGGTTCC CTCACCCTTG CAATTGGTTC AATGAAGCCA
    CTGGATTTTT TCCTCCTGAA TGTTCAAGAC CAGGGTGGCA GGGTTGAAGA CCTACTGTTC
    CATGTGGTGA GCGCCCCCAC CAATGGTCAG CTCGTGCTCT CAAGGAACGG AAAAGAGGCT
    CAGCTCGACA AGGCTGGCCA CTTCAGTTGG AGAGACGTGA AGGAGAAAAA AGTGCGTTTT
    GTGCACAGCA GAGAAAAGCC CAGGAAAGGT TACTTTTCTC TGAAAATTAG TGACCAGCAG
    TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTT
    CTGAGGAATG AGGTCCTCCA TATCAACAGA GGAGAGAGGG GAACCATCAC CACCCAGCTG
    CTTGACATCA GAGATGATGA CAACCCACAG GATGTGGTGG TCGAGGTGCT CGATCCTCCA
    CTTCACGGCC AGTTGCTCCA AACGCTCCAG TCCCCGGCGG CCCCTATCTA TCAGTTTCGT
    CTGGACGAGC TCTCTAGGGG CCTTGTCCAG TATGTTCACG ATGGCTCGGA TAGCACTTCT
    GACATCACAG TCTTGCAGGC CAATGATGGA CACTCCTTCC AAAATATACT GTTTCATGTG
    AGGACCGTGC CCAAGAATGA TCGAGGTCTT CGGCTTGTGG CTAATTCAAT GGTATGGGTT
    CCAGAAGGCG GGATGCTGCA GATTACTAAC AGAATCTTAC AGGCAGAAGC TCCAGGTGCC
    AGTGCCTCAG AAATCATCTA CAAAATCACA CAGGACCACC CCCAATTTGG GGAGGTGGTC
    CTTCTGGTTA ATATGCCTGC AGACAGCCCT GCAGATGGAG GGCAGCACCT GCCTGATGGG
    AGGACAGCAA CTCCCACCAG CACCTTCACC CAGCAAGATA TCAACGAAGG CATCGTGTGG
    TACAGGCACT CAGGGGTCCC AGCCCAGAGT GACTCCTTCC GCTTTCAGGT GTCCAGCGCC
    ACAGAGGCCC ACACCCATCT GGAGAGTCGC CTGTTCAACA TTGCCATCTT ACCTCACGCT
    CTGGAAACAC CCCAACACTT TCCAGGATCT CTCCACATGA CAGCTCGGGA AGATGGCCTG
    ACTGTGATTC AGCCTCATTC TCTCTCCTTT GTAAACTCTG AGAGGCCAAA CAGAAAGCTA
    ATATACAACA TCACTCTACC GCTGCATCCA AATCAAGGCA TCATCGAACA CAGGGACCAG
    CCTCACTCTC CCATCCGGTA TTTCTCCCAG GAAGACGTTA ACCAGGGCAA CATCATGTAC
    CGTCCTCCCA CTGCAGCCCC CCACCTCCAG GAGATCAGGG CCTTCTCCTT TGCTGGTCTC
    CCAGAATCGG TGAAATTCCA CTTCACAGTT TCTGATGGAG AGCACACGAG TCCAGAGATG
    GCCCTCACCA TTCACTTACT TCGCACAGAT CAGTACCCAC CACTGTTCCA GGTCACAGCT
    CCTCTGCTTG TGGTCAGCCC AGGAGGCAGC ACTTCTATAG AACTTCAGCT GGTAGTACGA
    GACATGGAAA CAGTGCCTGA AGAACTCCTC TTTGAGCTTC GGAAGCCTCC ACAGCATGGC
    GTGCTTATTA AGTACACAGC TGGGTCACCA GGGCCCATGG CCACAGGTGA CACTTTCACA
    TATGAGGATG TAGAGAAAAA TGTCCTGCGG TATCTACACG ATGGCTCATC TGCCCGGGAG
    GACAGCATGG AGATCTCAGT CACAGACGGC CTCTCAGCCA CAACAGTGGA CGTGAGGGTG
    GAGGTATCAC CGGCTGAGGC CCAGGGGCCC CGACTGGCTG CTGGTGCCTC GCTGAGCGTC
    ACGGTTGCCA GTAAAAGCAC AGCTGTGATC ACTCGGTCAC ACCTGGCTTA CGTGGATGAT
    TCTTCCTCCG ACCCAGAGAT CTGGATTCAG ATAACTTCTC TGCCCATGTA CGGTGCTCTC
    TTAAAAACCG CAGGACCTGA GGTGGAAGAA CTCTCAGAGA TTTCTAATTT CACAATGGAA
    GACATCAACA ACAAGAAAAT TAGGTATTCA GCCGCGTTTG AGACCGGTGG TCATCCAGTT
    ACTGACAGCT TCCACTTCTC TGTCTCTGAT ATGGACCACA ACCGTCTGGA CAATCAGAAG
    TTTACCATCA CCATCACACC CGTCAAGGAT CCACCCCCAG TCATTGCTTT TGCTGACCTT
    ATCACGGTTG ACGAGGGAGG GAGGACCCCA CTCTCTTTTC ACCATTTCTT CGCTGCTGCT
    GCTGAGGACA ATTTCCAGGA GGATGCCATC ATTAAGCTAA GCGCCCTGCC CCAGTACGGC
    TGCATCGAGA ACACGGGGAC AGGTGATCGG TTTGGCCCTG GAACTACCAG TGACCTAGAG
    GCATCGTTCC CTATTCAGGA TGTCCTGGAA AACTACATTT ACTACTTTCA GAGTGTTCAT
    GAAAGCATTG AACCAACCCA CGATATTTTC AGTTTTTATG TAAGTGATGG AAACAGTCGA
    TCAGAAGTCC ACAGCATCAA CATCACCATT GAGAGGAAGA ATGACGAGCC TCCCAGAATG
    ACCTTGAGGC CCCTTGGCGT GCAGCTGAGC TCAGGAGTGG TGATAAGCAA TTCTTCTTTG
    AGCCTGCAAG ACCTGGACAC TCCAGATAAT GAGCTGATTT TTGTATTGAC AAAAAAGCCT
    GGCCACGGTC ATCTGCTCTG GAGGCAAACT GCTTCTGAAC CTCTGGAGAA TGGGAGGGTT
    TTGGCCCAGG GCTCCTCCTT CACCTACCAA GATATCCTGG CTGGGCTAGT TGGGTACATG
    CCTGGTGGTC CAGGTGTGGC AGTGGATGAG TTCCAGTTCT CCCTCACTGA TGGCCTCCAC
    ACAGACACGG GGAGGATGGA GATATATATA GAACTGCCTA CAAGTGACAT CCCCAACCTG
    GCTGTAAACA GAGGCCTCCA GGTCTCAGCA GGGTCCGTAG CAACCATCAC AGATCGGCAC
    TTGAAAGTGA CGGCTCCTGA CTCAGATGAC CACCAGGTGA TGTACATCTT AAAGGAAGAC
    CCTGGAGTAG GGCGCCTGCA GATGGTAAAG CGTGACAGCC TGGAGCAGAT CTCCACTAGA
    GGCCCCATCC AAAGTTTCAC CCAGGCTGAC ATCAGTCAAG GCCTTATAGA ATACAGTCAT
    GGAAAAGGAG AATCTGGTGG GAACTTTGCT TTTAAATTTG ATGTGGTTGA TGGAGAAGGC
    AACAAACTGA TCGGCCAGTC ATTTTCTATC AGTGTTTCAG AAGACAAATC CCCACCAGTC
    ATCATCACCA ATAAAGGGCT GTTCTTGGAT GAAAACTCGG TGAAAAAGAT CACCACTCTG
    CAACTCTCTG CCACTGACCA GGAGGCGGAA CCCACAGAAC TGGTCTACCG AATCATCAGA
    GAGCCCCAGC TGGGCCGCCT GGAGCACACA GCATCACCAG GCATCCAGAT TAGTTCCTTT
    ACGCAAGCTG ACCTCACTTC AGGAAACGTT CAGTATATCC ACTCCAGTGA GACTGAGAAG
    CATTCAGATG CCTTCAGCTT TACGCTCTCT GATGGAGTCC ACGAGGTGAG TGAGAAACTC
    GCTTCCCTCC TTGATGGGTA TCAATTCAGC CTCAAACTCC GTGATGGTCT TCCTCACACC
    CTCCTGTACC CGCATCCCAA AGTTTTGCAC GTGGGGCAGC GAGTCATCCA CAGGGCGAAG
    AGTGATGCGG AAAGTCTGAG TCACCTACAG GGCGGAAAAG TGCAGTTGGC CACCTGTTGG
    CCACACATCA CACGTCTGGC TCCTGATACA GAGTTCCCTC TCCTCTTGTT GCCTCCACGA
    CAGGCCGAGT CTGTCACGTT CACGGTCGTG CAGNCCCCAC GCCACGGCAC CATCGAGCGA
    ACCGCCGATG GCAGCNNCCT TCCAGCTCAG TCCACCTTCA CCATGGAGGA CATCTACCAG
    AGCCGGGTCC GCTACAGCCA CGATGGCAGC AACTCCCTCA GAGACCGATT CACCNTTACC
    GTGGCTGATG GGACGAAATC CCTTCTTCAT GTTGAGGAAG GAGGAAAAGA GATCATAACA
    GCAGCACCTC AGCAGTTCTG GATAGACATC CTTCCAGTGG ATGATGGCAC CCCTAGGATT
    GTTACCAACC TCGGGCTCCA GTGGCTGGAG TACATGGATG GCCAGGCAAC CAACTTGATC
    ACCAAGAAGG AACTGCTGAC CATGGACCCG GACACGGAGG ACCTGCAACT TGTCTATGAG
    ATCACCACGG GCCCCAAGCA TGGCCACGTG GAGAACAAAC TGCAGCCTGG CAGGGCAGTG
    GCTACCTTCA CACAGGAGGA CGTGAACTTG GGGTTGATTC GTTACGTGTT GCATGAAGAG
    AAGATCCATG AGATGATGGA TAGTTTTCAG TTTCTGGTGA AAGATAGTAA ACCCAATGTG
    GTCAACGACA ACATCTTCCA TATCCAGTGG TCCCTCATCA GTTTTAAATA TACCAGCTAC
    AATGTCAGTG AGAAAGCAGG GTCAGTGAGT GTCACGGTGC AGAGGACGGG GAACCTGAAC
    CAGTATGCCA TTGTCCTGTG TCGCACGGAG CAAGGCACGG CCACCTCCAG CTCCCGGGTG
    GGCTCCCAGC CGGGACAGCA GGACTATGTG GAGTATGCTG GCCAGGTGCA GTTTGATGAG
    CGAGAGGACA CCAAGTCCTG CACCATCGTC ATCAACGATG ACAACGTGTT TGAGGACGTC
    GAGAGTTTCA CCGTGGAGCT CAGCATGCCA GCGTATGCCC TACTAGGGGA GTTCACCCAG
    GCGAAGGTCA TTATCAATGA CACTGAGGAC GAGCCTACAT TAGAGTTTGA TAAGAAGACC
    TACCGGGTCA ACGAGAGTGC TGGCTTTCTG TTTGCACCTA TTGAAAGAAA AGGGGATTCA
    AGCAGCATTG TATCTGTGAT TTGCTACACG GTCCCCAAGT CAGCTATGGG CAGTAGCCTC
    TATGCTCTAG AATCGGGCTC TGATTTTAAA TCTAGAGGGA TGTCTGCCGA GAGTCGAGTG
    ATATTTGGGC CTGGTGTCAC CATGTCCACC TGTGATGTCA TGCTCATCGA TGACAGCGAG
    TACGAAGAGG AAGAGGAGTT TGAGATTGCC CTGACAGGTG CTTCCGACAA CGCCCGCATC
    GGAAGAATGG CATCGGCCAA GGTGCTCATT GCTGGTCCCA ATGATGCTTC TACCGTGTCC
    TTGGGCAACA CGGCTTTTAC CATCAGTGAG GATGCAGGTA CAGTGAAGAT CCCAGTTATC
    CGCCACGGGA CTGACCTTTC CACCTTCACG TCTGTCTGGT GTGCCACGAG GCCCTCAGAC
    CCAGCGTCTG CCACCCCAGG AGTTGACTAC GTTCCCAGCT CTAGGAAGGT GGAGTTCGGA
    CCAGGTGTCA CTGAGCAGTA TTGCACCTTG ACCATCTTGG ATGACACTCA GTATCCAGTG
    ATTGAAGGGC TGGAGACATT TGTGGTTTTC CTCAGCTCCC CACAAGGGGC CGAACTGACC
    AAACCCTTCC AGGCTGTCAT TGCAATTAAT GACACATTCC AGGATGTGCC CAGCATGCAG
    TTTGCCAAGG ATTTGCTCCT GGTGAAGGAG AAGGAGGGCG TCCTGCATGT GCCCATCATT
    CGGAGTGGAG ACCTGAGCTA TGAGTCATCG GTGAGGTGCT ATACTCAGGG CCTTTCAGCT
    CAGGTCATGG AGGACTTTGA GGAGAGAAGA AACGCAGACT CTTCACGGAT TACGTTTCTC
    AAAGGGGAGA AAATGAAGAA CTGTACTGTC TATATACACG ATGACTCCAT GTTTGAGCCT
    GAGGAACAGT TCAGGGTCTA CCTTGGCCGT CCTCTTGGGA ACCACTGGAG TGGAGCCAGA
    GTTGGAAAGA ATCACATGGC CACCATCACC ATATCTAATG ATGAAGATGC CCCTACCATT
    GAGTTTGAAG AAGCTGCATA CCAAGTCCGG GAACCCTCAG GGCCAGATGC CACGGCCATT
    CTGAACGTCA AGGTGATCCG CAGAGGGGAT CAGAACAGAA CATCCAAGAT TCGCTGTAGC
    ACACGGGATG GGTCAGCCCA GTCTGGTGTG GATTATTACC CAAAGAGCCG AGTCTTGAAG
    TTCAGTCCTG GTGTGGATCA CATCTTCTTT AAAGTCGAGA TCCTGTCCAA TGAAGACCGG
    GAAGTGCATG AATCCTTCTC CTAGTCCTGG CCAGATGACC CANTGGAAGC AGTTCTTGGC
    GATGTGACCA CGGCTACAGT GACGATTCTA GACCAGGAGG CAGCTGGGAG TCTCATCCTG
    CCAGCACCGC CCATTGTCGT TACACTTGCT GACTATGACC ATGTGGAAGA GGTTACCAAG
    GAGGGAGTCA AGAAATCCCC CTCCCCGGGC TACCCGCTGA TCTGCGTCAC GCCCTGCGAC
    CCCCATTTCC CCAAATACGC TGTCATGAAG GAGCGCTGCA TTGAGGCTGG CATCAACCAG
    ACGTCTGTGC AGTTCAGCTG GGAAGTGGCC GCCCCCACTG ACGGCAACGG GGCCCGGTCT
    CCCTTCGAGA CCATCACCGA CAACACGCCA TTCACCAGTG TCAACCACAT GGTAGGTCTG
    GTTACAGAGA GAGAGACCTG GCCTTTCTCT TTGTTACTCC AGCTCTGTCA AATTCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319300.1
    CDS2231..12190
    Translation

    Target ORF information:

    RefSeq Version XM_015463814.1
    Organism Bos taurus(cattle)
    Definition Bos taurus extracellular matrix protein FRAS1-like (LOC107131184), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    ATGAGTGTCT TCCAAGTGTG GCTCGGGATG GCTCTCACAT TGGCGGAATT TGCAGCATTG 
    CCTCATCATT CTGAAGGTGC TTGTGTCTAT CAGGGTTCCT TGTTGGCGGA TGCCACCATT
    TGGAAGCCCG ATTCATGCCA GAACTGCCGT TGCCATGGTG ATATCGTTAT CTGCAAACCC
    GCTGTTTGCA GAAACCCTCA GTGTGCCTTT GAGAAGGGGG AAGTGCTTCA AATACCCGCC
    AACCAGTGCT GCCCTGAATG TGTGCCAAGG ATGCCTGGAT CTTGCCATTA TGAAAAGAAA
    ATTCACGGGC ACGGGACAGC GTGGGCCTCC TCCCCTTGCA GCACGTGCTC TTGCACTCAC
    GGGGAAGTCC GATGTGCCCC CCAACCATGC CCACCACTGA TGTGTGGACA CCAGGAGCTG
    GAATTCATCC CCGAAGGGAG CTGCTGCCCA GTCTGTGTGG GCCCTGGGAA ATCCTGTTCC
    TATGAAGGCC GTGTGTTTCA AGATGGAGAG GACTGGCCAT TGAGTCGGTG CGCCAAGTGT
    GTGTGTAGAA ATGGGGTTGC CCAGTGTTTT GCAGCTCAGT GTCCTCCTCT GTTTTGTAAC
    CAGGATGAAA CCGTCATCCG AGTCCCTGGA AAGTGCTGTC CACAGTGCTC TTCGCTGTCG
    TGCTCTGCTG CTGGCCAAGT GTACGAGCAT GGAGAGCAGT GGCAAGAAAA TGCCTGCACC
    ACGTGTACAT GTGACCAGGG CCAGGTCAGG TGTCACAAAC AGCCCTGCCC TCCATTAAGA
    TGCGAAAAGG GTCAGAGACG GGCTCGGCTT CCAGGGCAAT GCTGTGAGGA CTGTGTGTCT
    CCTGCTGGAA GCTGCTCGCA CAGAGGGCTT GTGCGATACC AGGATGATAT GTGGAAGGGC
    TCGGCCTGTG AGTTCTGCGT GTGCAACCGT GGCCAAGTGA CCTGCCAGAC TGCAGAGTGT
    GCCAAAGTGG AGTGTGCCCA GGGTGAAGAA TTAATTCACT TAGAGGGAAA ATGCTGTCCT
    GAATGCATTT CAAGGAATGG TTATTGTGTT TACGAAGAAA ATGCAGAATT TATGTCCTCA
    AATGCAAGTA AAGTTAAACG TATTCCAGAA GGAGAGAAGT GGGAGGATGG CCCTTGCAAG
    GTGTGTGAGT GCCGGGGGGC TCAGATAACT TGCTACGAGC CCTCTTGTCC TCCATGCCCG
    GTGGCCACAC TAGCCCAGGC GGTAAAGGGA CAGTGCTGTC CCGATTGCAC ATCAGTTCGT
    TGCCACCCAG ACTGCTTGAC CTGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAGGAT
    CCCACCAAGT TGCTGAGGAA TGGACAATGT GTGCACAGCT GTGGTCTGGG GTTTTACCAA
    GCTGGTGCTC TCTGTTTAGC CTGCCAGCCA CAGTGCTCCA CCTGCACCAG TGGGCTCGAG
    TGCTCGTCCT GCCAGCCTCC CCTGCTGATG CAGCAGGGCC AGTGTGTGTC TACCTGTGGG
    ATCGGCTTCT ACCAAGACCG CCACTCCTGT GCAGCCTGCC ACGAGTCCTG TGCCGCCTGC
    TGGGGTCCCA CAGAGAAGCA CTGCCTGGCC TGCAGAGACC CCCTCCACGT GCTAAGAGAG
    GGTGGCTGTG AGAGCAGCTG TGGCAACCGG TTTTATAACA AGCAGGGGAC CTGCAGTGCT
    TGTGATCAAT CCTGTAAGAG TTGTGGCCCT AGTAGTCCCA GATGTCTTAC CTGTGTGGAG
    AAGACCGTGT TGCATGATGG GAAATGCATT TCTGAATGCC CTGGTGGGTA CTATGCTGAT
    GCCACTGGGA GATGTAAAGT TTGTCACAAC TCATGTGCCA GCTGCTCAGG ACCCACGGCC
    GCTCACTGCA CCGCCTGCAT CCACCCCCAG GCCCTGCGCC AAGGCCACTG TCTGCCCAAC
    TGTGGAGAGG CCTTCTACCC TGACCATGGT GTCTGCAAAG CCTGTCATAC CTCTTGCTTG
    ACCTGTGTGG GTCCAGCACA CTCCCACTGC ACCCAGTGCA GGAAGCCAGA GGACGGACTG
    CAGTTTGAGC CGCTGTCAGG CGCAAACATC ACCTCAGGCG AGTGTCTGTC CCGGTGCAGA
    GCCCAGTTTT ACTTGGAGAT CACTGGACTG TGTGAAGCTT GCCACCCATC CTGTTCTGCG
    TGTGCAGGGA TGAGCCCACA CAACTGCACA GCCTGTTGGC CTTCCCACAT GCTGTTGGAT
    GGGGAGTGCC TCTCCCAGTG CCCAGATGGG TACTTCAACC AGGAAGGCAG TTGTACAGAG
    TGCCACCCCA CCTGCAGGCA ATGCCACGGC CCGTTGGAGT CTGACTGCAT CTCCTGCCAC
    CCTCACGTTC CTCTGACTGC TGGGAGCTGC AGGGCCCGCT GTAAAGAGGA GCAGTTCCTC
    AACCTCGTGG GCTACTGTGC TGACTGCCAC CCTCTGTGCC ATCACTGTGC AGCCAACCTC
    CGTGACACCG GGAGCGTCTG CCTGCAGTGC CAGAATGCCC GGAACCTGCT GCTGGGGGAC
    CGCTGTGTCC CTGATTGCCC TTCTGGGTAC TTCGTGGAGA GAGGAGCTTG TAAAAAATGT
    CACTCCTCCT GCAGGACCTG CCAGGGCAGG GGTCCGTTCT CCTGCTCCTC CTGTGACACT
    GGCCGTGTTC TGTCCCACCT TGGCACCTGC AGTATCGCCT GCTTCCCCGG GCACTATCTC
    GATGATAACC GTGTTTGTCA GCCATGCAAC ATGCATTGTG GACGCTGTGA TTCACAGGCC
    AGCTGTACCT CCTGCCGAGA TCCAAGCAAG GTCCTGCTCT TTGGGGACTG TCAGCATGAG
    AGCTGTGCCC CGCAATACTA TCTGGACTTC TCCACCAAAA CGTGCAAAGA GTGTGACTGG
    AGTTGCAATG TGTGCAGCGG GCCTCTTAGG ACAGACTGCC TCCAATGCAT GGACGGCTAT
    GTTCTCCAGG ACGGCGCCTG CGTGGAGCAG TGCTCAGCGT CATTTTACCG GGACCTGGGC
    CTCTGCAAGA GCTGTGAGAG CCACTGTCTC CAATGCCAAG GTCCCCATGA GTGCACCCGC
    TGTGAAGAGC CGTTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGCGG GAAGGGTTTC
    TTTGCAGACC ACGCTAACCA CAAATGCACA GCCTGCCCTA AGGGGTGCTT GCAGTGCAGC
    CGCAGAGACC GGTGTCACCT CTGTGACCAG GGCTTCTTTC TCAAGAGCGG TCTTTGTCTT
    TCCAACTGTG TTCCTGGCTT CTCCGCCCAC TCTTCTAATG AGACATGTGC TGGCAAAATA
    CACACCCCTA GTCTTCACGT GAATGGTTCC CTCACCCTTG CAATTGGTTC AATGAAGCCA
    CTGGATTTTT TCCTCCTGAA TGTTCAAGAC CAGGGTGGCA GGGTTGAAGA CCTACTGTTC
    CATGTGGTGA GCGCCCCCAC CAATGGTCAG CTCGTGCTCT CAAGGAACGG AAAAGAGGCT
    CAGCTCGACA AGGCTGGCCA CTTCAGTTGG AGAGACGTGA AGGAGAAAAA AGTGCGTTTT
    GTGCACAGCA GAGAAAAGCC CAGGAAAGGT TACTTTTCTC TGAAAATTAG TGACCAGCAG
    TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTT
    CTGAGGAATG AGGTCCTCCA TATCAACAGA GGAGAGAGGG GAACCATCAC CACCCAGCTG
    CTTGACATCA GAGATGATGA CAACCCACAG GATGTGGTGG TCGAGGTGCT CGATCCTCCA
    CTTCACGGCC AGTTGCTCCA AACGCTCCAG TCCCCGGCGG CCCCTATCTA TCAGTTTCGT
    CTGGACGAGC TCTCTAGGGG CCTTGTCCAG TATGTTCACG ATGGCTCGGA TAGCACTTCT
    GACATCACAG TCTTGCAGGC CAATGATGGA CACTCCTTCC AAAATATACT GTTTCATGTG
    AGGACCGTGC CCAAGAATGA TCGAGGTCTT CGGCTTGTGG CTAATTCAAT GGTATGGGTT
    CCAGAAGGCG GGATGCTGCA GATTACTAAC AGAATCTTAC AGGCAGAAGC TCCAGGTGCC
    AGTGCCTCAG AAATCATCTA CAAAATCACA CAGGACCACC CCCAATTTGG GGAGGTGGTC
    CTTCTGGTTA ATATGCCTGC AGACAGCCCT GCAGATGGAG GGCAGCACCT GCCTGATGGG
    AGGACAGCAA CTCCCACCAG CACCTTCACC CAGCAAGATA TCAACGAAGG CATCGTGTGG
    TACAGGCACT CAGGGGTCCC AGCCCAGAGT GACTCCTTCC GCTTTCAGGT GTCCAGCGCC
    ACAGAGGCCC ACACCCATCT GGAGAGTCGC CTGTTCAACA TTGCCATCTT ACCTCACGCT
    CTGGAAACAC CCCAACACTT TCCAGGATCT CTCCACATGA CAGCTCGGGA AGATGGCCTG
    ACTGTGATTC AGCCTCATTC TCTCTCCTTT GTAAACTCTG AGAGGCCAAA CAGAAAGCTA
    ATATACAACA TCACTCTACC GCTGCATCCA AATCAAGGCA TCATCGAACA CAGGGACCAG
    CCTCACTCTC CCATCCGGTA TTTCTCCCAG GAAGACGTTA ACCAGGGCAA CATCATGTAC
    CGTCCTCCCA CTGCAGCCCC CCACCTCCAG GAGATCAGGG CCTTCTCCTT TGCTGGTCTC
    CCAGAATCGG TGAAATTCCA CTTCACAGTT TCTGATGGAG AGCACACGAG TCCAGAGATG
    GCCCTCACCA TTCACTTACT TCGCACAGAT CAGTACCCAC CACTGTTCCA GGTCACAGCT
    CCTCTGCTTG TGGTCAGCCC AGGAGGCAGC ACTTCTATAG AACTTCAGCT GGTAGTACGA
    GACATGGAAA CAGTGCCTGA AGAACTCCTC TTTGAGCTTC GGAAGCCTCC ACAGCATGGC
    GTGCTTATTA AGTACACAGC TGGGTCACCA GGGCCCATGG CCACAGGTGA CACTTTCACA
    TATGAGGATG TAGAGAAAAA TGTCCTGCGG TATCTACACG ATGGCTCATC TGCCCGGGAG
    GACAGCATGG AGATCTCAGT CACAGACGGC CTCTCAGCCA CAACAGTGGA CGTGAGGGTG
    GAGGTATCAC CGGCTGAGGC CCAGGGGCCC CGACTGGCTG CTGGTGCCTC GCTGAGCGTC
    ACGGTTGCCA GTAAAAGCAC AGCTGTGATC ACTCGGTCAC ACCTGGCTTA CGTGGATGAT
    TCTTCCTCCG ACCCAGAGAT CTGGATTCAG ATAACTTCTC TGCCCATGTA CGGTGCTCTC
    TTAAAAACCG CAGGACCTGA GGTGGAAGAA CTCTCAGAGA TTTCTAATTT CACAATGGAA
    GACATCAACA ACAAGAAAAT TAGGTATTCA GCCGCGTTTG AGACCGGTGG TCATCCAGTT
    ACTGACAGCT TCCACTTCTC TGTCTCTGAT ATGGACCACA ACCGTCTGGA CAATCAGAAG
    TTTACCATCA CCATCACACC CGTCAAGGAT CCACCCCCAG TCATTGCTTT TGCTGACCTT
    ATCACGGTTG ACGAGGGAGG GAGGACCCCA CTCTCTTTTC ACCATTTCTT CGCTGCTGCT
    GCTGAGGACA ATTTCCAGGA GGATGCCATC ATTAAGCTAA GCGCCCTGCC CCAGTACGGC
    TGCATCGAGA ACACGGGGAC AGGTGATCGG TTTGGCCCTG GAACTACCAG TGACCTAGAG
    GCATCGTTCC CTATTCAGGA TGTCCTGGAA AACTACATTT ACTACTTTCA GAGTGTTCAT
    GAAAGCATTG AACCAACCCA CGATATTTTC AGTTTTTATG TAAGTGATGG AAACAGTCGA
    TCAGAAGTCC ACAGCATCAA CATCACCATT GAGAGGAAGA ATGACGAGCC TCCCAGAATG
    ACCTTGAGGC CCCTTGGCGT GCAGCTGAGC TCAGGAGTGG TGATAAGCAA TTCTTCTTTG
    AGCCTGCAAG ACCTGGACAC TCCAGATAAT GAGCTGATTT TTGTATTGAC AAAAAAGCCT
    GGCCACGGTC ATCTGCTCTG GAGGCAAACT GCTTCTGAAC CTCTGGAGAA TGGGAGGGTT
    TTGGCCCAGG GCTCCTCCTT CACCTACCAA GATATCCTGG CTGGGCTAGT TGGGTACATG
    CCTGGTGGTC CAGGTGTGGC AGTGGATGAG TTCCAGTTCT CCCTCACTGA TGGCCTCCAC
    ACAGACACGG GGAGGATGGA GATATATATA GAACTGCCTA CAAGTGACAT CCCCAACCTG
    GCTGTAAACA GAGGCCTCCA GGTCTCAGCA GGGTCCGTAG CAACCATCAC AGATCGGCAC
    TTGAAAGTGA CGGCTCCTGA CTCAGATGAC CACCAGGTGA TGTACATCTT AAAGGAAGAC
    CCTGGAGTAG GGCGCCTGCA GATGGTAAAG CGTGACAGCC TGGAGCAGAT CTCCACTAGA
    GGCCCCATCC AAAGTTTCAC CCAGGCTGAC ATCAGTCAAG GCCTTATAGA ATACAGTCAT
    GGAAAAGGAG AATCTGGTGG GAACTTTGCT TTTAAATTTG ATGTGGTTGA TGGAGAAGGC
    AACAAACTGA TCGGCCAGTC ATTTTCTATC AGTGTTTCAG AAGACAAATC CCCACCAGTC
    ATCATCACCA ATAAAGGGCT GTTCTTGGAT GAAAACTCGG TGAAAAAGAT CACCACTCTG
    CAACTCTCTG CCACTGACCA GGAGGCGGAA CCCACAGAAC TGGTCTACCG AATCATCAGA
    GAGCCCCAGC TGGGCCGCCT GGAGCACACA GCATCACCAG GCATCCAGAT TAGTTCCTTT
    ACGCAAGCTG ACCTCACTTC AGGAAACGTT CAGTATATCC ACTCCAGTGA GACTGAGAAG
    CATTCAGATG CCTTCAGCTT TACGCTCTCT GATGGAGTCC ACGAGGTGAG TGAGAAACTC
    GCTTCCCTCC TTGATGGGTA TCAATTCAGC CTCAAACTCC GTGATGGTCT TCCTCACACC
    CTCCTGTACC CGCATCCCAA AGTTTTGCAC GTGGGGCAGC GAGTCATCCA CAGGGCGAAG
    AGTGATGCGG AAAGTCTGAG TCACCTACAG GGCGGAAAAG TGCAGTTGGC CACCTGTTGG
    CCACACATCA CACGTCTGGC TCCTGATACA GAGTTCCCTC TCCTCTTGTT GCCTCCACGA
    CAGGCCGAGT CTGTCACGTT CACGGTCGTG CAGNCCCCAC GCCACGGCAC CATCGAGCGA
    ACCGCCGATG GCAGCNNCCT TCCAGCTCAG TCCACCTTCA CCATGGAGGA CATCTACCAG
    AGCCGGGTCC GCTACAGCCA CGATGGCAGC AACTCCCTCA GAGACCGATT CACCNTTACC
    GTGGCTGATG GGACGAAATC CCTTCTTCAT GTTGAGGAAG GAGGAAAAGA GATCATAACA
    GCAGCACCTC AGCAGTTCTG GATAGACATC CTTCCAGTGG ATGATGGCAC CCCTAGGATT
    GTTACCAACC TCGGGCTCCA GTGGCTGGAG TACATGGATG GCCAGGCAAC CAACTTGATC
    ACCAAGAAGG AACTGCTGAC CATGGACCCG GACACGGAGG ACCTGCAACT TGTCTATGAG
    ATCACCACGG GCCCCAAGCA TGGCCACGTG GAGAACAAAC TGCAGCCTGG CAGGGCAGTG
    GCTACCTTCA CACAGGAGGA CGTGAACTTG GGGTTGATTC GTTACGTGTT GCATGAAGAG
    AAGATCCATG AGATGATGGA TAGTTTTCAG TTTCTGGTGA AAGATAGTAA ACCCAATGTG
    GTCAACGACA ACATCTTCCA TATCCAGTGG TCCCTCATCA GTTTTAAATA TACCAGCTAC
    AATGTCAGTG AGAAAGCAGG GTCAGTGAGT GTCACGGTGC AGAGGACGGG GAACCTGAAC
    CAGTATGCCA TTGTCCTGTG TCGCACGGAG CAAGGCACGG CCACCTCCAG CTCCCGGGTG
    GGCTCCCAGC CGGGACAGCA GGACTATGTG GAGTATGCTG GCCAGGTGCA GTTTGATGAG
    CGAGAGGACA CCAAGTCCTG CACCATCGTC ATCAACGATG ACAACGTGTT TGAGGACGTC
    GAGAGTTTCA CCGTGGAGCT CAGCATGCCA GCGTATGCCC TACTAGGGGA GTTCACCCAG
    GCGAAGGTCA TTATCAATGA CACTGAGGAC GAGCCTACAT TAGAGTTTGA TAAGAAGACC
    TACCGGGTCA ACGAGAGTGC TGGCTTTCTG TTTGCACCTA TTGAAAGAAA AGGGGATTCA
    AGCAGCATTG TATCTGTGAT TTGCTACACG GTCCCCAAGT CAGCTATGGG CAGTAGCCTC
    TATGCTCTAG AATCGGGCTC TGATTTTAAA TCTAGAGGGA TGTCTGCCGA GAGTCGAGTG
    ATATTTGGGC CTGGTGTCAC CATGTCCACC TGTGATGTCA TGCTCATCGA TGACAGCGAG
    TACGAAGAGG AAGAGGAGTT TGAGATTGCC CTGACAGGTG CTTCCGACAA CGCCCGCATC
    GGAAGAATGG CATCGGCCAA GGTGCTCATT GCTGGTCCCA ATGATGCTTC TACCGTGTCC
    TTGGGCAACA CGGCTTTTAC CATCAGTGAG GATGCAGGTA CAGTGAAGAT CCCAGTTATC
    CGCCACGGGA CTGACCTTTC CACCTTCACG TCTGTCTGGT GTGCCACGAG GCCCTCAGAC
    CCAGCGTCTG CCACCCCAGG AGTTGACTAC GTTCCCAGCT CTAGGAAGGT GGAGTTCGGA
    CCAGGTGTCA CTGAGCAGTA TTGCACCTTG ACCATCTTGG ATGACACTCA GTATCCAGTG
    ATTGAAGGGC TGGAGACATT TGTGGTTTTC CTCAGCTCCC CACAAGGGGC CGAACTGACC
    AAACCCTTCC AGGCTGTCAT TGCAATTAAT GACACATTCC AGGATGTGCC CAGCATGCAG
    TTTGCCAAGG ATTTGCTCCT GGTGAAGGAG AAGGAGGGCG TCCTGCATGT GCCCATCATT
    CGGAGTGGAG ACCTGAGCTA TGAGTCATCG GTGAGGTGCT ATACTCAGGG CCTTTCAGCT
    CAGGTCATGG AGGACTTTGA GGAGAGAAGA AACGCAGACT CTTCACGGAT TACGTTTCTC
    AAAGGGGAGA AAATGAAGAA CTGTACTGTC TATATACACG ATGACTCCAT GTTTGAGCCT
    GAGGAACAGT TCAGGGTCTA CCTTGGCCGT CCTCTTGGGA ACCACTGGAG TGGAGCCAGA
    GTTGGAAAGA ATCACATGGC CACCATCACC ATATCTAATG ATGAAGATGC CCCTACCATT
    GAGTTTGAAG AAGCTGCATA CCAAGTCCGG GAACCCTCAG GGCCAGATGC CACGGCCATT
    CTGAACGTCA AGGTGATCCG CAGAGGGGAT CAGAACAGAA CATCCAAGAT TCGCTGTAGC
    ACACGGGATG GGTCAGCCCA GTCTGGTGTG GATTATTACC CAAAGAGCCG AGTCTTGAAG
    TTCAGTCCTG GTGTGGATCA CATCTTCTTT AAAGTCGAGA TCCTGTCCAA TGAAGACCGG
    GAAGTGCATG AATCCTTCTC CTAGTCCTGG CCAGATGACC CANTGGAAGC AGTTCTTGGC
    GATGTGACCA CGGCTACAGT GACGATTCTA GACCAGGAGG CAGCTGGGAG TCTCATCCTG
    CCAGCACCGC CCATTGTCGT TACACTTGCT GACTATGACC ATGTGGAAGA GGTTACCAAG
    GAGGGAGTCA AGAAATCCCC CTCCCCGGGC TACCCGCTGA TCTGCGTCAC GCCCTGCGAC
    CCCCATTTCC CCAAATACGC TGTCATGAAG GAGCGCTGCA TTGAGGCTGG CATCAACCAG
    ACGTCTGTGC AGTTCAGCTG GGAAGTGGCC GCCCCCACTG ACGGCAACGG GGCCCGGTCT
    CCCTTCGAGA CCATCACCGA CAACACGCCA TTCACCAGTG TCAACCACAT GGTAGGTCTG
    GTTACAGAGA GAGAGACCTG GCCTTTCTCT TTGTTACTCC AGCTCTGTCA AATTCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90954
    Clone ID Related Accession (Same CDS sequence) XM_015471687.1
    Accession Version XM_015471687.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product extracellular matrix protein FRAS1-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGAGTGTCT TCCAAGTGTG GCTCGGGATG GCTCTCACAT TGGCGGAATT TGCAGCATTG 
    CCTCATCATT CTGAAGGTGC TTGTGTCTAT CAGGGTTCCT TGTTGGCGGA TGCCACCATT
    TGGAAGCCCG ATTCATGCCA GAACTGCCGT TGCCATGGTG ATATCGTTAT CTGCAAACCC
    GCTGTTTGCA GAAACCCTCA GTGTGCCTTT GAGAAGGGGG AAGTGCTTCA AATACCCGCC
    AACCAGTGCT GCCCTGAATG TGTGCCAAGG ATGCCTGGAT CTTGCCATTA TGAAAAGAAA
    ATTCACGGGC ACGGGACAGC GTGGGCCTCC TCCCCTTGCA GCACGTGCTC TTGCACTCAC
    GGGGAAGTCC GATGTGCCCC CCAACCATGC CCACCACTGA TGTGTGGACA CCAGGAGCTG
    GAATTCATCC CCGAAGGGAG CTGCTGCCCA GTCTGTGTGG GCCCTGGGAA ATCCTGTTCC
    TATGAAGGCC GTGTGTTTCA AGATGGAGAG GACTGGCCAT TGAGTCGGTG CGCCAAGTGT
    GTGTGTAGAA ATGGGGTTGC CCAGTGTTTT GCAGCTCAGT GTCCTCCTCT GTTTTGTAAC
    CAGGATGAAA CCGTCATCCG AGTCCCTGGA AAGTGCTGTC CACAGTGCTC TTCGCTGTCG
    TGCTCTGCTG CTGGCCAAGT GTACGAGCAT GGAGAGCAGT GGCAAGAAAA TGCCTGCACC
    ACGTGTACAT GTGACCAGGG CCAGGTCAGG TGTCACAAAC AGCCCTGCCC TCCATTAAGA
    TGCGAAAAGG GTCAGAGACG GGCTCGGCTT CCAGGGCAAT GCTGTGAGGA CTGTGTGTCT
    CCTGCTGGAA GCTGCTCGCA CAGAGGGCTT GTGCGATACC AGGATGATAT GTGGAAGGGC
    TCGGCCTGTG AGTTCTGCGT GTGCAACCGT GGCCAAGTGA CCTGCCAGAC TGCAGAGTGT
    GCCAAAGTGG AGTGTGCCCA GGGTGAAGAA TTAATTCACT TAGAGGGAAA ATGCTGTCCT
    GAATGCATTT CAAGGAATGG TTATTGTGTT TACGAAGAAA ATGCAGAATT TATGTCCTCA
    AATGCAAGTA AAGTTAAACG TATTCCAGAA GGAGAGAAGT GGGAGGATGG CCCTTGCAAG
    GTGTGTGAGT GCCGGGGGGC TCAGATAACT TGCTACGAGC CCTCTTGTCC TCCATGCCCG
    GTGGCCACAC TAGCCCAGGC GGTAAAGGGA CAGTGCTGTC CCGATTGCAC ATCAGTTCGT
    TGCCACCCAG ACTGCTTGAC CTGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAGGAT
    CCCACCAAGT TGCTGAGGAA TGGACAATGT GTGCACAGCT GTGGTCTGGG GTTTTACCAA
    GCTGGTGCTC TCTGTTTAGC CTGCCAGCCA CAGTGCTCCA CCTGCACCAG TGGGCTCGAG
    TGCTCGTCCT GCCAGCCTCC CCTGCTGATG CAGCAGGGCC AGTGTGTGTC TACCTGTGGG
    ATCGGCTTCT ACCAAGACCG CCACTCCTGT GCAGCCTGCC ACGAGTCCTG TGCCGCCTGC
    TGGGGTCCCA CAGAGAAGCA CTGCCTGGCC TGCAGAGACC CCCTCCACGT GCTAAGAGAG
    GGTGGCTGTG AGAGCAGCTG TGGCAACCGG TTTTATAACA AGCAGGGGAC CTGCAGTGCT
    TGTGATCAAT CCTGTAAGAG TTGTGGCCCT AGTAGTCCCA GATGTCTTAC CTGTGTGGAG
    AAGACCGTGT TGCATGATGG GAAATGCATT TCTGAATGCC CTGGTGGGTA CTATGCTGAT
    GCCACTGGGA GATGTAAAGT TTGTCACAAC TCATGTGCCA GCTGCTCAGG ACCCACGGCC
    GCTCACTGCA CCGCCTGCAT CCACCCCCAG GCCCTGCGCC AAGGCCACTG TCTGCCCAAC
    TGTGGAGAGG CCTTCTACCC TGACCATGGT GTCTGCAAAG CCTGTCATAC CTCTTGCTTG
    ACCTGTGTGG GTCCAGCACA CTCCCACTGC ACCCAGTGCA GGAAGCCAGA GGACGGACTG
    CAGTTTGAGC CGCTGTCAGG CGCAAACATC ACCTCAGGCG AGTGTCTGTC CCGGTGCAGA
    GCCCAGTTTT ACTTGGAGAT CACTGGACTG TGTGAAGCTT GCCACCCATC CTGTTCTGCG
    TGTGCAGGGA TGAGCCCACA CAACTGCACA GCCTGTTGGC CTTCCCACAT GCTGTTGGAT
    GGGGAGTGCC TCTCCCAGTG CCCAGATGGG TACTTCAACC AGGAAGGCAG TTGTACAGAG
    TGCCACCCCA CCTGCAGGCA ATGCCACGGC CCGTTGGAGT CTGACTGCAT CTCCTGCCAC
    CCTCACGTTC CTCTGACTGC TGGGAGCTGC AGGGCCCGCT GTAAAGAGGA GCAGTTCCTC
    AACCTCGTGG GCTACTGTGC TGACTGCCAC CCTCTGTGCC ATCACTGTGC AGCCAACCTC
    CGTGACACCG GGAGCGTCTG CCTGCAGTGC CAGAATGCCC GGAACCTGCT GCTGGGGGAC
    CGCTGTGTCC CTGATTGCCC TTCTGGGTAC TTCGTGGAGA GAGGAGCTTG TAAAAAATGT
    CACTCCTCCT GCAGGACCTG CCAGGGCAGG GGTCCGTTCT CCTGCTCCTC CTGTGACACT
    GGCCGTGTTC TGTCCCACCT TGGCACCTGC AGTATCGCCT GCTTCCCCGG GCACTATCTC
    GATGATAACC GTGTTTGTCA GCCATGCAAC ATGCATTGTG GACGCTGTGA TTCACAGGCC
    AGCTGTACCT CCTGCCGAGA TCCAAGCAAG GTCCTGCTCT TTGGGGACTG TCAGCATGAG
    AGCTGTGCCC CGCAATACTA TCTGGACTTC TCCACCAAAA CGTGCAAAGA GTGTGACTGG
    AGTTGCAATG TGTGCAGCGG GCCTCTTAGG ACAGACTGCC TCCAATGCAT GGACGGCTAT
    GTTCTCCAGG ACGGCGCCTG CGTGGAGCAG TGCTCAGCGT CATTTTACCG GGACCTGGGC
    CTCTGCAAGA GCTGTGAGAG CCACTGTCTC CAATGCCAAG GTCCCCATGA GTGCACCCGC
    TGTGAAGAGC CGTTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGCGG GAAGGGTTTC
    TTTGCAGACC ACGCTAACCA CAAATGCACA GCCTGCCCTA AGGGGTGCTT GCAGTGCAGC
    CGCAGAGACC GGTGTCACCT CTGTGACCAG GGCTTCTTTC TCAAGAGCGG TCTTTGTCTT
    TCCAACTGTG TTCCTGGCTT CTCCGCCCAC TCTTCTAATG AGACATGTGC TGGCAAAATA
    CACACCCCTA GTCTTCACGT GAATGGTTCC CTCACCCTTG CAATTGGTTC AATGAAGCCA
    CTGGATTTTT TCCTCCTGAA TGTTCAAGAC CAGGGTGGCA GGGTTGAAGA CCTACTGTTC
    CATGTGGTGA GCGCCCCCAC CAATGGTCAG CTCGTGCTCT CAAGGAACGG AAAAGAGGCT
    CAGCTCGACA AGGCTGGCCA CTTCAGTTGG AGAGACGTGA AGGAGAAAAA AGTGCGTTTT
    GTGCACAGCA GAGAAAAGCC CAGGAAAGGT TACTTTTCTC TGAAAATTAG TGACCAGCAG
    TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTT
    CTGAGGAATG AGGTCCTCCA TATCAACAGA GGAGAGAGGG GAACCATCAC CACCCAGCTG
    CTTGACATCA GAGATGATGA CAACCCACAG GATGTGGTGG TCGAGGTGCT CGATCCTCCA
    CTTCACGGCC AGTTGCTCCA AACGCTCCAG TCCCCGGCGG CCCCTATCTA TCAGTTTCGT
    CTGGACGAGC TCTCTAGGGG CCTTGTCCAG TATGTTCACG ATGGCTCGGA TAGCACTTCT
    GACATCACAG TCTTGCAGGC CAATGATGGA CACTCCTTCC AAAATATACT GTTTCATGTG
    AGGACCGTGC CCAAGAATGA TCGAGGTCTT CGGCTTGTGG CTAATTCAAT GGTATGGGTT
    CCAGAAGGCG GGATGCTGCA GATTACTAAC AGAATCTTAC AGGCAGAAGC TCCAGGTGCC
    AGTGCCTCAG AAATCATCTA CAAAATCACA CAGGACCACC CCCAATTTGG GGAGGTGGTC
    CTTCTGGTTA ATATGCCTGC AGACAGCCCT GCAGATGGAG GGCAGCACCT GCCTGATGGG
    AGGACAGCAA CTCCCACCAG CACCTTCACC CAGCAAGATA TCAACGAAGG CATCGTGTGG
    TACAGGCACT CAGGGGTCCC AGCCCAGAGT GACTCCTTCC GCTTTCAGGT GTCCAGCGCC
    ACAGAGGCCC ACACCCATCT GGAGAGTCGC CTGTTCAACA TTGCCATCTT ACCTCACGCT
    CTGGAAACAC CCCAACACTT TCCAGGATCT CTCCACATGA CAGCTCGGGA AGATGGCCTG
    ACTGTGATTC AGCCTCATTC TCTCTCCTTT GTAAACTCTG AGAGGCCAAA CAGAAAGCTA
    ATATACAACA TCACTCTACC GCTGCATCCA AATCAAGGCA TCATCGAACA CAGGGACCAG
    CCTCACTCTC CCATCCGGTA TTTCTCCCAG GAAGACGTTA ACCAGGGCAA CATCATGTAC
    CGTCCTCCCA CTGCAGCCCC CCACCTCCAG GAGATCAGGG CCTTCTCCTT TGCTGGTCTC
    CCAGAATCGG TGAAATTCCA CTTCACAGTT TCTGATGGAG AGCACACGAG TCCAGAGATG
    GCCCTCACCA TTCACTTACT TCGCACAGAT CAGTACCCAC CACTGTTCCA GGTCACAGCT
    CCTCTGCTTG TGGTCAGCCC AGGAGGCAGC ACTTCTATAG AACTTCAGCT GGTAGTACGA
    GACATGGAAA CAGTGCCTGA AGAACTCCTC TTTGAGCTTC GGAAGCCTCC ACAGCATGGC
    GTGCTTATTA AGTACACAGC TGGGTCACCA GGGCCCATGG CCACAGGTGA CACTTTCACA
    TATGAGGATG TAGAGAAAAA TGTCCTGCGG TATCTACACG ATGGCTCATC TGCCCGGGAG
    GACAGCATGG AGATCTCAGT CACAGACGGC CTCTCAGCCA CAACAGTGGA CGTGAGGGTG
    GAGGTATCAC CGGCTGAGGC CCAGGGGCCC CGACTGGCTG CTGGTGCCTC GCTGAGCGTC
    ACGGTTGCCA GTAAAAGCAC AGCTGTGATC ACTCGGTCAC ACCTGGCTTA CGTGGATGAT
    TCTTCCTCCG ACCCAGAGAT CTGGATTCAG ATAACTTCTC TGCCCATGTA CGGTGCTCTC
    TTAAAAACCG CAGGACCTGA GGTGGAAGAA CTCTCAGAGA TTTCTAATTT CACAATGGAA
    GACATCAACA ACAAGAAAAT TAGGTATTCA GCCGCGTTTG AGACCGGTGG TCATCCAGTT
    ACTGACAGCT TCCACTTCTC TGTCTCTGAT ATGGACCACA ACCGTCTGGA CAATCAGAAG
    TTTACCATCA CCATCACACC CGTCAAGGAT CCACCCCCAG TCATTGCTTT TGCTGACCTT
    ATCACGGTTG ACGAGGGAGG GAGGACCCCA CTCTCTTTTC ACCATTTCTT CGCTGCTGCT
    GCTGAGGACA ATTTCCAGGA GGATGCCATC ATTAAGCTAA GCGCCCTGCC CCAGTACGGC
    TGCATCGAGA ACACGGGGAC AGGTGATCGG TTTGGCCCTG GAACTACCAG TGACCTAGAG
    GCATCGTTCC CTATTCAGGA TGTCCTGGAA AACTACATTT ACTACTTTCA GAGTGTTCAT
    GAAAGCATTG AACCAACCCA CGATATTTTC AGTTTTTATG TAAGTGATGG AAACAGTCGA
    TCAGAAGTCC ACAGCATCAA CATCACCATT GAGAGGAAGA ATGACGAGCC TCCCAGAATG
    ACCTTGAGGC CCCTTGGCGT GCAGCTGAGC TCAGGAGTGG TGATAAGCAA TTCTTCTTTG
    AGCCTGCAAG ACCTGGACAC TCCAGATAAT GAGCTGATTT TTGTATTGAC AAAAAAGCCT
    GGCCACGGTC ATCTGCTCTG GAGGCAAACT GCTTCTGAAC CTCTGGAGAA TGGGAGGGTT
    TTGGCCCAGG GCTCCTCCTT CACCTACCAA GATATCCTGG CTGGGCTAGT TGGGTACATG
    CCTGGTGGTC CAGGTGTGGC AGTGGATGAG TTCCAGTTCT CCCTCACTGA TGGCCTCCAC
    ACAGACACGG GGAGGATGGA GATATATATA GAACTGCCTA CAAGTGACAT CCCCAACCTG
    GCTGTAAACA GAGGCCTCCA GGTCTCAGCA GGGTCCGTAG CAACCATCAC AGATCGGCAC
    TTGAAAGTGA CGGCTCCTGA CTCAGATGAC CACCAGGTGA TGTACATCTT AAAGGAAGAC
    CCTGGAGTAG GGCGCCTGCA GATGGTAAAG CGTGACAGCC TGGAGCAGAT CTCCACTAGA
    GGCCCCATCC AAAGTTTCAC CCAGGCTGAC ATCAGTCAAG GCCTTATAGA ATACAGTCAT
    GGAAAAGGAG AATCTGGTGG GAACTTTGCT TTTAAATTTG ATGTGGTTGA TGGAGAAGGC
    AACAAACTGA TCGGCCAGTC ATTTTCTATC AGTGTTTCAG AAGACAAATC CCCACCAGTC
    ATCATCACCA ATAAAGGGCT GTTCTTGGAT GAAAACTCGG TGAAAAAGAT CACCACTCTG
    CAACTCTCTG CCACTGACCA GGAGGCGGAA CCCACAGAAC TGGTCTACCG AATCATCAGA
    GAGCCCCAGC TGGGCCGCCT GGAGCACACA GCATCACCAG GCATCCAGAT TAGTTCCTTT
    ACGCAAGCTG ACCTCACTTC AGGAAACGTT CAGTATATCC ACTCCAGTGA GACTGAGAAG
    CATTCAGATG CCTTCAGCTT TACGCTCTCT GATGGAGTCC ACGAGGTGAG TGAGAAACTC
    GCTTCCCTCC TTGATGGGTA TCAATTCAGC CTCAAACTCC GTGATGGTCT TCCTCACACC
    CTCCTGTACC CGCATCCCAA AGTTTTGCAC GTGGGGCAGC GAGTCATCCA CAGGGCGAAG
    AGTGATGCGG AAAGTCTGAG TCACCTACAG GGCGGAAAAG TGCAGATCAT AACAGCAGCA
    CCTCAGCAGT TCTGGATAGA CATCCTTCCA GTGGATGATG GCACCCCTAG GATTGTTACC
    AACCTCGGGC TCCAGTGGCT GGAGTACATG GATGGCCAGG CAACCAACTT GATCACCAAG
    AAGGAACTGC TGACCATGGA CCCGGACACG GAGGACCTGC AACTTGTCTA TGAGATCACC
    ACGGGCCCCA AGCATGGCCA CGTGGAGAAC AAACTGCAGC CTGGCAGGGC AGTGGCTACC
    TTCACACAGG AGGACGTGAA CTTGGGGTTG ATTCGTTACG TGTTGCATGA AGAGAAGATC
    CATGAGATGA TGGATAGTTT TCAGTTTCTG GTGAAAGATA GTAAACCCAA TGTGGTCAAC
    GACAACATCT TCCATATCCA GTGGTCCCTC ATCAGTTTTA AATATACCAG CTACAATGTC
    AGTGAGAAAG CAGGGTCAGT GAGTGTCACG GTGCAGAGGA CGGGGAACCT GAACCAGTAT
    GCCATTGTCC TGTGTCGCAC GGAGCAAGGC ACGGCCACCT CCAGCTCCCG GGTGGGCTCC
    CAGCCGGGAC AGCAGGACTA TGTGGAGTAT GCTGGCCAGG TGCAGTTTGA TGAGCGAGAG
    GACACCAAGT CCTGCACCAT CGTCATCAAC GATGACAACG TGTTTGAGGA CGTCGAGAGT
    TTCACCGTGG AGCTCAGCAT GCCAGCGTAT GCCCTACTAG GGGAGTTCAC CCAGGCGAAG
    GTCATTATCA ATGACACTGA GGACGAGCCT ACATTAGAGT TTGATAAGAA GACCTACCGG
    GTCAACGAGA GTGCTGGCTT TCTGTTTGCA CCTATTGAAA GAAAAGGGGA TTCAAGCAGC
    ATTGTATCTG TGATTTGCTA CACGGTCCCC AAGTCAGCTA TGGGCAGTAG CCTCTATGCT
    CTAGAATCGG GCTCTGATTT TAAATCTAGA GGGATGTCTG CCGAGAGTCG AGTGATATTT
    GGGCCTGGTG TCACCATGTC CACCTGTGAT GTCATGCTCA TCGATGACAG CGAGTACGAA
    GAGGAAGAGG AGTTTGAGAT TGCCCTGACA GGTGCTTCCG ACAACGCCCG CATCGGAAGA
    ATGGCATCGG CCAAGGTGCT CATTGCTGGT CCCAATGATG CTTCTACCGT GTCCTTGGGC
    AACACGGCTT TTACCATCAG TGAGGATGCA GGTACAGTGA AGATCCCAGT TATCCGCCAC
    GGGACTGACC TTTCCACCTT CACGTCTGTC TGGTGTGCCA CGAGGCCCTC AGACCCAGCG
    TCTGCCACCC CAGGAGTTGA CTACGTTCCC AGCTCTAGGA AGGTGGAGTT CGGACCAGGT
    GTCACTGAGC AGTATTGCAC CTTGACCATC TTGGATGACA CTCAGTATCC AGTGATTGAA
    GGGCTGGAGA CATTTGTGGT TTTCCTCAGC TCCCCACAAG GGGCCGAACT GACCAAACCC
    TTCCAGGCTG TCATTGCAAT TAATGACACA TTCCAGGATG TGCCCAGCAT GCAGTTTGCC
    AAGGATTTGC TCCTGGTGAA GGAGAAGGAG GGCGTCCTGC ATGTGCCCAT CATTCGGAGT
    GGAGACCTGA GCTATGAGTC ATCGGTGAGG TGCTATACTC AGGGCCTTTC AGCTCAGGTC
    ATGGAGGACT TTGAGGAGAG AAGAAACGCA GACTCTTCAC GGATTACGTT TCTCAAAGGG
    GAGAAAATGA AGAACTGTAC TGTCTATATA CACGATGACT CCATGTTTGA GCCTGAGGAA
    CAGTTCAGGG TCTACCTTGG CCGTCCTCTT GGGAACCACT GGAGTGGAGC CAGAGTTGGA
    AAGAATCACA TGGCCACCAT CACCATATCT AATGATGAAG ATGCCCCTAC CATTGAGTTT
    GAAGAAGCTG CATACCAAGT CCGGGAACCC TCAGGGCCAG ATGCCACGGC CATTCTGAAC
    GTCAAGGTGA TCCGCAGAGG GGATCAGAAC AGAACATCCA AGATTCGCTG TAGCACACGG
    GATGGGTCAG CCCAGTCTGG TGTGGATTAT TACCCAAAGA GCCGAGTCTT GAAGTTCAGT
    CCTGGTAATT CACCGCCCAT TGTCGTTACA CTTGCTGACT ATGACCATGT GGAAGAGGTT
    ACCAAGGAGG GAGTCAAGAA ATCCCCCTCC CCGGGCTACC CGCTGATCTG CGTCACGCCC
    TGCGACCCCC ATTTCCCCAA ATACGCTGTC ATGAAGGAGC GCTGCATTGA GGCTGGCATC
    AACCAGACGT CTGTGCAGTT CAGCTGGGAA GTGGCCGCCC CCACTGACGG CAACGGGGCC
    CGGTCTCCCT TCGAGACCAT CACCGACAAC ACGCCATTCA CCAGTGTCAA CCACATGGTA
    GGTCTGGTTA CAGAGAGAGA GACCTGGCCT TTCTCTTTGT TACTCCAGCT CTGTCAAATT
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327173.1
    CDS2231..11716
    Translation

    Target ORF information:

    RefSeq Version XM_015471687.1
    Organism Bos taurus(cattle)
    Definition Bos taurus extracellular matrix protein FRAS1-like (LOC107131184), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471687.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGAGTGTCT TCCAAGTGTG GCTCGGGATG GCTCTCACAT TGGCGGAATT TGCAGCATTG 
    CCTCATCATT CTGAAGGTGC TTGTGTCTAT CAGGGTTCCT TGTTGGCGGA TGCCACCATT
    TGGAAGCCCG ATTCATGCCA GAACTGCCGT TGCCATGGTG ATATCGTTAT CTGCAAACCC
    GCTGTTTGCA GAAACCCTCA GTGTGCCTTT GAGAAGGGGG AAGTGCTTCA AATACCCGCC
    AACCAGTGCT GCCCTGAATG TGTGCCAAGG ATGCCTGGAT CTTGCCATTA TGAAAAGAAA
    ATTCACGGGC ACGGGACAGC GTGGGCCTCC TCCCCTTGCA GCACGTGCTC TTGCACTCAC
    GGGGAAGTCC GATGTGCCCC CCAACCATGC CCACCACTGA TGTGTGGACA CCAGGAGCTG
    GAATTCATCC CCGAAGGGAG CTGCTGCCCA GTCTGTGTGG GCCCTGGGAA ATCCTGTTCC
    TATGAAGGCC GTGTGTTTCA AGATGGAGAG GACTGGCCAT TGAGTCGGTG CGCCAAGTGT
    GTGTGTAGAA ATGGGGTTGC CCAGTGTTTT GCAGCTCAGT GTCCTCCTCT GTTTTGTAAC
    CAGGATGAAA CCGTCATCCG AGTCCCTGGA AAGTGCTGTC CACAGTGCTC TTCGCTGTCG
    TGCTCTGCTG CTGGCCAAGT GTACGAGCAT GGAGAGCAGT GGCAAGAAAA TGCCTGCACC
    ACGTGTACAT GTGACCAGGG CCAGGTCAGG TGTCACAAAC AGCCCTGCCC TCCATTAAGA
    TGCGAAAAGG GTCAGAGACG GGCTCGGCTT CCAGGGCAAT GCTGTGAGGA CTGTGTGTCT
    CCTGCTGGAA GCTGCTCGCA CAGAGGGCTT GTGCGATACC AGGATGATAT GTGGAAGGGC
    TCGGCCTGTG AGTTCTGCGT GTGCAACCGT GGCCAAGTGA CCTGCCAGAC TGCAGAGTGT
    GCCAAAGTGG AGTGTGCCCA GGGTGAAGAA TTAATTCACT TAGAGGGAAA ATGCTGTCCT
    GAATGCATTT CAAGGAATGG TTATTGTGTT TACGAAGAAA ATGCAGAATT TATGTCCTCA
    AATGCAAGTA AAGTTAAACG TATTCCAGAA GGAGAGAAGT GGGAGGATGG CCCTTGCAAG
    GTGTGTGAGT GCCGGGGGGC TCAGATAACT TGCTACGAGC CCTCTTGTCC TCCATGCCCG
    GTGGCCACAC TAGCCCAGGC GGTAAAGGGA CAGTGCTGTC CCGATTGCAC ATCAGTTCGT
    TGCCACCCAG ACTGCTTGAC CTGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAGGAT
    CCCACCAAGT TGCTGAGGAA TGGACAATGT GTGCACAGCT GTGGTCTGGG GTTTTACCAA
    GCTGGTGCTC TCTGTTTAGC CTGCCAGCCA CAGTGCTCCA CCTGCACCAG TGGGCTCGAG
    TGCTCGTCCT GCCAGCCTCC CCTGCTGATG CAGCAGGGCC AGTGTGTGTC TACCTGTGGG
    ATCGGCTTCT ACCAAGACCG CCACTCCTGT GCAGCCTGCC ACGAGTCCTG TGCCGCCTGC
    TGGGGTCCCA CAGAGAAGCA CTGCCTGGCC TGCAGAGACC CCCTCCACGT GCTAAGAGAG
    GGTGGCTGTG AGAGCAGCTG TGGCAACCGG TTTTATAACA AGCAGGGGAC CTGCAGTGCT
    TGTGATCAAT CCTGTAAGAG TTGTGGCCCT AGTAGTCCCA GATGTCTTAC CTGTGTGGAG
    AAGACCGTGT TGCATGATGG GAAATGCATT TCTGAATGCC CTGGTGGGTA CTATGCTGAT
    GCCACTGGGA GATGTAAAGT TTGTCACAAC TCATGTGCCA GCTGCTCAGG ACCCACGGCC
    GCTCACTGCA CCGCCTGCAT CCACCCCCAG GCCCTGCGCC AAGGCCACTG TCTGCCCAAC
    TGTGGAGAGG CCTTCTACCC TGACCATGGT GTCTGCAAAG CCTGTCATAC CTCTTGCTTG
    ACCTGTGTGG GTCCAGCACA CTCCCACTGC ACCCAGTGCA GGAAGCCAGA GGACGGACTG
    CAGTTTGAGC CGCTGTCAGG CGCAAACATC ACCTCAGGCG AGTGTCTGTC CCGGTGCAGA
    GCCCAGTTTT ACTTGGAGAT CACTGGACTG TGTGAAGCTT GCCACCCATC CTGTTCTGCG
    TGTGCAGGGA TGAGCCCACA CAACTGCACA GCCTGTTGGC CTTCCCACAT GCTGTTGGAT
    GGGGAGTGCC TCTCCCAGTG CCCAGATGGG TACTTCAACC AGGAAGGCAG TTGTACAGAG
    TGCCACCCCA CCTGCAGGCA ATGCCACGGC CCGTTGGAGT CTGACTGCAT CTCCTGCCAC
    CCTCACGTTC CTCTGACTGC TGGGAGCTGC AGGGCCCGCT GTAAAGAGGA GCAGTTCCTC
    AACCTCGTGG GCTACTGTGC TGACTGCCAC CCTCTGTGCC ATCACTGTGC AGCCAACCTC
    CGTGACACCG GGAGCGTCTG CCTGCAGTGC CAGAATGCCC GGAACCTGCT GCTGGGGGAC
    CGCTGTGTCC CTGATTGCCC TTCTGGGTAC TTCGTGGAGA GAGGAGCTTG TAAAAAATGT
    CACTCCTCCT GCAGGACCTG CCAGGGCAGG GGTCCGTTCT CCTGCTCCTC CTGTGACACT
    GGCCGTGTTC TGTCCCACCT TGGCACCTGC AGTATCGCCT GCTTCCCCGG GCACTATCTC
    GATGATAACC GTGTTTGTCA GCCATGCAAC ATGCATTGTG GACGCTGTGA TTCACAGGCC
    AGCTGTACCT CCTGCCGAGA TCCAAGCAAG GTCCTGCTCT TTGGGGACTG TCAGCATGAG
    AGCTGTGCCC CGCAATACTA TCTGGACTTC TCCACCAAAA CGTGCAAAGA GTGTGACTGG
    AGTTGCAATG TGTGCAGCGG GCCTCTTAGG ACAGACTGCC TCCAATGCAT GGACGGCTAT
    GTTCTCCAGG ACGGCGCCTG CGTGGAGCAG TGCTCAGCGT CATTTTACCG GGACCTGGGC
    CTCTGCAAGA GCTGTGAGAG CCACTGTCTC CAATGCCAAG GTCCCCATGA GTGCACCCGC
    TGTGAAGAGC CGTTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGCGG GAAGGGTTTC
    TTTGCAGACC ACGCTAACCA CAAATGCACA GCCTGCCCTA AGGGGTGCTT GCAGTGCAGC
    CGCAGAGACC GGTGTCACCT CTGTGACCAG GGCTTCTTTC TCAAGAGCGG TCTTTGTCTT
    TCCAACTGTG TTCCTGGCTT CTCCGCCCAC TCTTCTAATG AGACATGTGC TGGCAAAATA
    CACACCCCTA GTCTTCACGT GAATGGTTCC CTCACCCTTG CAATTGGTTC AATGAAGCCA
    CTGGATTTTT TCCTCCTGAA TGTTCAAGAC CAGGGTGGCA GGGTTGAAGA CCTACTGTTC
    CATGTGGTGA GCGCCCCCAC CAATGGTCAG CTCGTGCTCT CAAGGAACGG AAAAGAGGCT
    CAGCTCGACA AGGCTGGCCA CTTCAGTTGG AGAGACGTGA AGGAGAAAAA AGTGCGTTTT
    GTGCACAGCA GAGAAAAGCC CAGGAAAGGT TACTTTTCTC TGAAAATTAG TGACCAGCAG
    TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTT
    CTGAGGAATG AGGTCCTCCA TATCAACAGA GGAGAGAGGG GAACCATCAC CACCCAGCTG
    CTTGACATCA GAGATGATGA CAACCCACAG GATGTGGTGG TCGAGGTGCT CGATCCTCCA
    CTTCACGGCC AGTTGCTCCA AACGCTCCAG TCCCCGGCGG CCCCTATCTA TCAGTTTCGT
    CTGGACGAGC TCTCTAGGGG CCTTGTCCAG TATGTTCACG ATGGCTCGGA TAGCACTTCT
    GACATCACAG TCTTGCAGGC CAATGATGGA CACTCCTTCC AAAATATACT GTTTCATGTG
    AGGACCGTGC CCAAGAATGA TCGAGGTCTT CGGCTTGTGG CTAATTCAAT GGTATGGGTT
    CCAGAAGGCG GGATGCTGCA GATTACTAAC AGAATCTTAC AGGCAGAAGC TCCAGGTGCC
    AGTGCCTCAG AAATCATCTA CAAAATCACA CAGGACCACC CCCAATTTGG GGAGGTGGTC
    CTTCTGGTTA ATATGCCTGC AGACAGCCCT GCAGATGGAG GGCAGCACCT GCCTGATGGG
    AGGACAGCAA CTCCCACCAG CACCTTCACC CAGCAAGATA TCAACGAAGG CATCGTGTGG
    TACAGGCACT CAGGGGTCCC AGCCCAGAGT GACTCCTTCC GCTTTCAGGT GTCCAGCGCC
    ACAGAGGCCC ACACCCATCT GGAGAGTCGC CTGTTCAACA TTGCCATCTT ACCTCACGCT
    CTGGAAACAC CCCAACACTT TCCAGGATCT CTCCACATGA CAGCTCGGGA AGATGGCCTG
    ACTGTGATTC AGCCTCATTC TCTCTCCTTT GTAAACTCTG AGAGGCCAAA CAGAAAGCTA
    ATATACAACA TCACTCTACC GCTGCATCCA AATCAAGGCA TCATCGAACA CAGGGACCAG
    CCTCACTCTC CCATCCGGTA TTTCTCCCAG GAAGACGTTA ACCAGGGCAA CATCATGTAC
    CGTCCTCCCA CTGCAGCCCC CCACCTCCAG GAGATCAGGG CCTTCTCCTT TGCTGGTCTC
    CCAGAATCGG TGAAATTCCA CTTCACAGTT TCTGATGGAG AGCACACGAG TCCAGAGATG
    GCCCTCACCA TTCACTTACT TCGCACAGAT CAGTACCCAC CACTGTTCCA GGTCACAGCT
    CCTCTGCTTG TGGTCAGCCC AGGAGGCAGC ACTTCTATAG AACTTCAGCT GGTAGTACGA
    GACATGGAAA CAGTGCCTGA AGAACTCCTC TTTGAGCTTC GGAAGCCTCC ACAGCATGGC
    GTGCTTATTA AGTACACAGC TGGGTCACCA GGGCCCATGG CCACAGGTGA CACTTTCACA
    TATGAGGATG TAGAGAAAAA TGTCCTGCGG TATCTACACG ATGGCTCATC TGCCCGGGAG
    GACAGCATGG AGATCTCAGT CACAGACGGC CTCTCAGCCA CAACAGTGGA CGTGAGGGTG
    GAGGTATCAC CGGCTGAGGC CCAGGGGCCC CGACTGGCTG CTGGTGCCTC GCTGAGCGTC
    ACGGTTGCCA GTAAAAGCAC AGCTGTGATC ACTCGGTCAC ACCTGGCTTA CGTGGATGAT
    TCTTCCTCCG ACCCAGAGAT CTGGATTCAG ATAACTTCTC TGCCCATGTA CGGTGCTCTC
    TTAAAAACCG CAGGACCTGA GGTGGAAGAA CTCTCAGAGA TTTCTAATTT CACAATGGAA
    GACATCAACA ACAAGAAAAT TAGGTATTCA GCCGCGTTTG AGACCGGTGG TCATCCAGTT
    ACTGACAGCT TCCACTTCTC TGTCTCTGAT ATGGACCACA ACCGTCTGGA CAATCAGAAG
    TTTACCATCA CCATCACACC CGTCAAGGAT CCACCCCCAG TCATTGCTTT TGCTGACCTT
    ATCACGGTTG ACGAGGGAGG GAGGACCCCA CTCTCTTTTC ACCATTTCTT CGCTGCTGCT
    GCTGAGGACA ATTTCCAGGA GGATGCCATC ATTAAGCTAA GCGCCCTGCC CCAGTACGGC
    TGCATCGAGA ACACGGGGAC AGGTGATCGG TTTGGCCCTG GAACTACCAG TGACCTAGAG
    GCATCGTTCC CTATTCAGGA TGTCCTGGAA AACTACATTT ACTACTTTCA GAGTGTTCAT
    GAAAGCATTG AACCAACCCA CGATATTTTC AGTTTTTATG TAAGTGATGG AAACAGTCGA
    TCAGAAGTCC ACAGCATCAA CATCACCATT GAGAGGAAGA ATGACGAGCC TCCCAGAATG
    ACCTTGAGGC CCCTTGGCGT GCAGCTGAGC TCAGGAGTGG TGATAAGCAA TTCTTCTTTG
    AGCCTGCAAG ACCTGGACAC TCCAGATAAT GAGCTGATTT TTGTATTGAC AAAAAAGCCT
    GGCCACGGTC ATCTGCTCTG GAGGCAAACT GCTTCTGAAC CTCTGGAGAA TGGGAGGGTT
    TTGGCCCAGG GCTCCTCCTT CACCTACCAA GATATCCTGG CTGGGCTAGT TGGGTACATG
    CCTGGTGGTC CAGGTGTGGC AGTGGATGAG TTCCAGTTCT CCCTCACTGA TGGCCTCCAC
    ACAGACACGG GGAGGATGGA GATATATATA GAACTGCCTA CAAGTGACAT CCCCAACCTG
    GCTGTAAACA GAGGCCTCCA GGTCTCAGCA GGGTCCGTAG CAACCATCAC AGATCGGCAC
    TTGAAAGTGA CGGCTCCTGA CTCAGATGAC CACCAGGTGA TGTACATCTT AAAGGAAGAC
    CCTGGAGTAG GGCGCCTGCA GATGGTAAAG CGTGACAGCC TGGAGCAGAT CTCCACTAGA
    GGCCCCATCC AAAGTTTCAC CCAGGCTGAC ATCAGTCAAG GCCTTATAGA ATACAGTCAT
    GGAAAAGGAG AATCTGGTGG GAACTTTGCT TTTAAATTTG ATGTGGTTGA TGGAGAAGGC
    AACAAACTGA TCGGCCAGTC ATTTTCTATC AGTGTTTCAG AAGACAAATC CCCACCAGTC
    ATCATCACCA ATAAAGGGCT GTTCTTGGAT GAAAACTCGG TGAAAAAGAT CACCACTCTG
    CAACTCTCTG CCACTGACCA GGAGGCGGAA CCCACAGAAC TGGTCTACCG AATCATCAGA
    GAGCCCCAGC TGGGCCGCCT GGAGCACACA GCATCACCAG GCATCCAGAT TAGTTCCTTT
    ACGCAAGCTG ACCTCACTTC AGGAAACGTT CAGTATATCC ACTCCAGTGA GACTGAGAAG
    CATTCAGATG CCTTCAGCTT TACGCTCTCT GATGGAGTCC ACGAGGTGAG TGAGAAACTC
    GCTTCCCTCC TTGATGGGTA TCAATTCAGC CTCAAACTCC GTGATGGTCT TCCTCACACC
    CTCCTGTACC CGCATCCCAA AGTTTTGCAC GTGGGGCAGC GAGTCATCCA CAGGGCGAAG
    AGTGATGCGG AAAGTCTGAG TCACCTACAG GGCGGAAAAG TGCAGATCAT AACAGCAGCA
    CCTCAGCAGT TCTGGATAGA CATCCTTCCA GTGGATGATG GCACCCCTAG GATTGTTACC
    AACCTCGGGC TCCAGTGGCT GGAGTACATG GATGGCCAGG CAACCAACTT GATCACCAAG
    AAGGAACTGC TGACCATGGA CCCGGACACG GAGGACCTGC AACTTGTCTA TGAGATCACC
    ACGGGCCCCA AGCATGGCCA CGTGGAGAAC AAACTGCAGC CTGGCAGGGC AGTGGCTACC
    TTCACACAGG AGGACGTGAA CTTGGGGTTG ATTCGTTACG TGTTGCATGA AGAGAAGATC
    CATGAGATGA TGGATAGTTT TCAGTTTCTG GTGAAAGATA GTAAACCCAA TGTGGTCAAC
    GACAACATCT TCCATATCCA GTGGTCCCTC ATCAGTTTTA AATATACCAG CTACAATGTC
    AGTGAGAAAG CAGGGTCAGT GAGTGTCACG GTGCAGAGGA CGGGGAACCT GAACCAGTAT
    GCCATTGTCC TGTGTCGCAC GGAGCAAGGC ACGGCCACCT CCAGCTCCCG GGTGGGCTCC
    CAGCCGGGAC AGCAGGACTA TGTGGAGTAT GCTGGCCAGG TGCAGTTTGA TGAGCGAGAG
    GACACCAAGT CCTGCACCAT CGTCATCAAC GATGACAACG TGTTTGAGGA CGTCGAGAGT
    TTCACCGTGG AGCTCAGCAT GCCAGCGTAT GCCCTACTAG GGGAGTTCAC CCAGGCGAAG
    GTCATTATCA ATGACACTGA GGACGAGCCT ACATTAGAGT TTGATAAGAA GACCTACCGG
    GTCAACGAGA GTGCTGGCTT TCTGTTTGCA CCTATTGAAA GAAAAGGGGA TTCAAGCAGC
    ATTGTATCTG TGATTTGCTA CACGGTCCCC AAGTCAGCTA TGGGCAGTAG CCTCTATGCT
    CTAGAATCGG GCTCTGATTT TAAATCTAGA GGGATGTCTG CCGAGAGTCG AGTGATATTT
    GGGCCTGGTG TCACCATGTC CACCTGTGAT GTCATGCTCA TCGATGACAG CGAGTACGAA
    GAGGAAGAGG AGTTTGAGAT TGCCCTGACA GGTGCTTCCG ACAACGCCCG CATCGGAAGA
    ATGGCATCGG CCAAGGTGCT CATTGCTGGT CCCAATGATG CTTCTACCGT GTCCTTGGGC
    AACACGGCTT TTACCATCAG TGAGGATGCA GGTACAGTGA AGATCCCAGT TATCCGCCAC
    GGGACTGACC TTTCCACCTT CACGTCTGTC TGGTGTGCCA CGAGGCCCTC AGACCCAGCG
    TCTGCCACCC CAGGAGTTGA CTACGTTCCC AGCTCTAGGA AGGTGGAGTT CGGACCAGGT
    GTCACTGAGC AGTATTGCAC CTTGACCATC TTGGATGACA CTCAGTATCC AGTGATTGAA
    GGGCTGGAGA CATTTGTGGT TTTCCTCAGC TCCCCACAAG GGGCCGAACT GACCAAACCC
    TTCCAGGCTG TCATTGCAAT TAATGACACA TTCCAGGATG TGCCCAGCAT GCAGTTTGCC
    AAGGATTTGC TCCTGGTGAA GGAGAAGGAG GGCGTCCTGC ATGTGCCCAT CATTCGGAGT
    GGAGACCTGA GCTATGAGTC ATCGGTGAGG TGCTATACTC AGGGCCTTTC AGCTCAGGTC
    ATGGAGGACT TTGAGGAGAG AAGAAACGCA GACTCTTCAC GGATTACGTT TCTCAAAGGG
    GAGAAAATGA AGAACTGTAC TGTCTATATA CACGATGACT CCATGTTTGA GCCTGAGGAA
    CAGTTCAGGG TCTACCTTGG CCGTCCTCTT GGGAACCACT GGAGTGGAGC CAGAGTTGGA
    AAGAATCACA TGGCCACCAT CACCATATCT AATGATGAAG ATGCCCCTAC CATTGAGTTT
    GAAGAAGCTG CATACCAAGT CCGGGAACCC TCAGGGCCAG ATGCCACGGC CATTCTGAAC
    GTCAAGGTGA TCCGCAGAGG GGATCAGAAC AGAACATCCA AGATTCGCTG TAGCACACGG
    GATGGGTCAG CCCAGTCTGG TGTGGATTAT TACCCAAAGA GCCGAGTCTT GAAGTTCAGT
    CCTGGTAATT CACCGCCCAT TGTCGTTACA CTTGCTGACT ATGACCATGT GGAAGAGGTT
    ACCAAGGAGG GAGTCAAGAA ATCCCCCTCC CCGGGCTACC CGCTGATCTG CGTCACGCCC
    TGCGACCCCC ATTTCCCCAA ATACGCTGTC ATGAAGGAGC GCTGCATTGA GGCTGGCATC
    AACCAGACGT CTGTGCAGTT CAGCTGGGAA GTGGCCGCCC CCACTGACGG CAACGGGGCC
    CGGTCTCCCT TCGAGACCAT CACCGACAAC ACGCCATTCA CCAGTGTCAA CCACATGGTA
    GGTCTGGTTA CAGAGAGAGA GACCTGGCCT TTCTCTTTGT TACTCCAGCT CTGTCAAATT
    CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.