LOC100847952 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100847952
Entrez Gene ID	100847952
Full Name	collagen alpha-1(XXVIII) chain
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024990988.1	XP_024846756.1	collagen alpha-1(XXVIII) chain isoform X2
XM_003585948.4	XP_003585996.2	collagen alpha-1(XXVIII) chain
XM_024990987.1	XP_024846755.1	collagen alpha-1(XXVIII) chain isoform X1
XM_024990989.1	XP_024846757.1	collagen alpha-1(XXVIII) chain isoform X3
XM_015462953.1	XP_015318439.1	collagen alpha-1(XXVIII) chain

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	col28a1l	XP_005172134.1
Bos taurus (cattle)	COL28A1	XP_003585996.2
Xenopus tropicalis (tropical clawed frog)	LOC100490088	XP_002936803.2
Danio rerio (zebrafish)	col28a1a	NP_001153314.1

Gene Ontology

The following LOC100847952 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100847952 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa18429	XM_015462953.1 Latest version!	Bos taurus collagen alpha-1(XXVIII) chain (LOC100847952), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa18429	XM_003585948.4 Latest version!	Bos taurus collagen alpha-1(XXVIII) chain (LOC100847952), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa03776	XM_024990987.1 Latest version!	Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa233910	XM_024990988.1 Latest version!	Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa60405	XM_024990989.1 Latest version!	Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100847952 ( XM_003585948.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585996.2 Bos taurus collagen alpha-1(XXVIII) chain (LOC100847952), mRNA.

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CloneID	OBa18429
Clone ID Related Accession (Same CDS sequence)	XM_003585948.4 , XM_015462953.1
Accession Version	XM_003585948.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXVIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003585948.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGACA CCTCTGAGAA ACAGAGCTAA GGTTGGAGTC 
CTGCATTTTA CAGCAGCTGG AGAGAGAGAG CATTCCGTAC AGCATGAAGA CAGGCTGAAT 
CCAGAGTACC ATGTTAGGGG AGCCGAGGAG GAAGGGCTCC AAGAAGAAAG CAATGGGTGG 
TGTCAAATAC ACAAGCAGAG CAAGGAAAAT ATGCGTGAAC TGGATTTCTG GCATCGAGGG 
GATATGGGGC TTGTAGAAGG CTATTGCAGA AGAGTGCTTG AAAATATCCC AGAAACGTCT 
TCTGTCCCCA GTAAGCCAGA AGATAGCACT CTAGCAAGGA CAACTCTCAA AGTGATTAAG 
AACAGCGGAG GTCAGCATCC TACCTCTTGC TTTAAGAGGA ATCACTACAA CTTATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585996.2
CDS	181..2220
Translation	MWNRRFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFIVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGMKVAVLMTDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPRGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGEKGSQGAIGPQGEAGPSIKGDKGDMGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKTPLRNRAKVGVLHFTAAGEREHSVQHEDRLNPEYHVRGAEEEGLQEESNGWCQIHKQSKENMRELDFWHRGDMGLVEGYCRRVLENIPETSSVPSKPEDSTLARTTLKVIKNSGGQHPTSCFKRNHYNLC

Target ORF information:

RefSeq Version	XM_003585948.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen alpha-1(XXVIII) chain (LOC100847952), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585948.4

ORF Insert Sequence:

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGACA CCTCTGAGAA ACAGAGCTAA GGTTGGAGTC 
CTGCATTTTA CAGCAGCTGG AGAGAGAGAG CATTCCGTAC AGCATGAAGA CAGGCTGAAT 
CCAGAGTACC ATGTTAGGGG AGCCGAGGAG GAAGGGCTCC AAGAAGAAAG CAATGGGTGG 
TGTCAAATAC ACAAGCAGAG CAAGGAAAAT ATGCGTGAAC TGGATTTCTG GCATCGAGGG 
GATATGGGGC TTGTAGAAGG CTATTGCAGA AGAGTGCTTG AAAATATCCC AGAAACGTCT 
TCTGTCCCCA GTAAGCCAGA AGATAGCACT CTAGCAAGGA CAACTCTCAA AGTGATTAAG 
AACAGCGGAG GTCAGCATCC TACCTCTTGC TTTAAGAGGA ATCACTACAA CTTATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100847952 ( XM_015462953.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318439.1 Bos taurus collagen alpha-1(XXVIII) chain (LOC100847952), mRNA.

Price & Availability↑

CloneID	OBa18429
Clone ID Related Accession (Same CDS sequence)	XM_003585948.4 , XM_015462953.1
Accession Version	XM_015462953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXVIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644695.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGACA CCTCTGAGAA ACAGAGCTAA GGTTGGAGTC 
CTGCATTTTA CAGCAGCTGG AGAGAGAGAG CATTCCGTAC AGCATGAAGA CAGGCTGAAT 
CCAGAGTACC ATGTTAGGGG AGCCGAGGAG GAAGGGCTCC AAGAAGAAAG CAATGGGTGG 
TGTCAAATAC ACAAGCAGAG CAAGGAAAAT ATGCGTGAAC TGGATTTCTG GCATCGAGGG 
GATATGGGGC TTGTAGAAGG CTATTGCAGA AGAGTGCTTG AAAATATCCC AGAAACGTCT 
TCTGTCCCCA GTAAGCCAGA AGATAGCACT CTAGCAAGGA CAACTCTCAA AGTGATTAAG 
AACAGCGGAG GTCAGCATCC TACCTCTTGC TTTAAGAGGA ATCACTACAA CTTATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318439.1
CDS	181..2220
Translation	MWNRRFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFIVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGMKVAVLMTDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPRGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGEKGSQGAIGPQGEAGPSIKGDKGDMGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKTPLRNRAKVGVLHFTAAGEREHSVQHEDRLNPEYHVRGAEEEGLQEESNGWCQIHKQSKENMRELDFWHRGDMGLVEGYCRRVLENIPETSSVPSKPEDSTLARTTLKVIKNSGGQHPTSCFKRNHYNLC

Target ORF information:

RefSeq Version	XM_015462953.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen alpha-1(XXVIII) chain (LOC100847952), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462953.1

ORF Insert Sequence:

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGACA CCTCTGAGAA ACAGAGCTAA GGTTGGAGTC 
CTGCATTTTA CAGCAGCTGG AGAGAGAGAG CATTCCGTAC AGCATGAAGA CAGGCTGAAT 
CCAGAGTACC ATGTTAGGGG AGCCGAGGAG GAAGGGCTCC AAGAAGAAAG CAATGGGTGG 
TGTCAAATAC ACAAGCAGAG CAAGGAAAAT ATGCGTGAAC TGGATTTCTG GCATCGAGGG 
GATATGGGGC TTGTAGAAGG CTATTGCAGA AGAGTGCTTG AAAATATCCC AGAAACGTCT 
TCTGTCCCCA GTAAGCCAGA AGATAGCACT CTAGCAAGGA CAACTCTCAA AGTGATTAAG 
AACAGCGGAG GTCAGCATCC TACCTCTTGC TTTAAGAGGA ATCACTACAA CTTATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100847952 ( XM_024990987.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846755.1 Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa03776
Clone ID Related Accession (Same CDS sequence)	XM_024990987.1
Accession Version	XM_024990987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXVIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCCGGA GGGCCAAAGG GTGATCCAGG AATTATGGGC 
CCTGTTGGGA TGCCTGGAAT ATCAATTCCT GGACCACCTG GACCAAAAGG AGACAGAGGT 
GGACCTGGGA TGCCTGGATT TAAAGGAGAA CCTGGAATTG CTATTCGAGG ACCAAAGGGT 
GCCCAAGGCC CGCAGGGACC AGTGGGTGCT CCAGGACTCA AAGGTGACGG CTATCCTGGT 
GTGCCCGGCC CTCGAGGAAT CCCAGGACCT CCTGGATCAA GGGGATTACC TGGAGTTGGA 
GACACTGGAG CAAAGGGAGA TCCTGGCGTC AGAGGCCCTC CAGGCCCCCC TGGGCCTCGG 
GGCATAGGAA CCCCAGGGCC AAAGGGTGAC ATCGGGCAGA AAGGCTTGCC TGGCCCTCCT 
GGCTCCCCTG GCTATGGATT ACAAGGAATC AAAGGGGAGC AAGGACCTCA GGGCTTCCCA 
GGGCCAAAGG GCATGACAGG CCATGGCCTC CCTGGCCAGA AGGGAGAGCA CGGAGAACGG 
GGCGATGTGG GAAAGAAAGG TGATAAAGGG GAAATTGGAG ATCCCGGACC CCAAGGAGAA 
CAGGGCTTAC AAGGACCAAA AGGAGACCAA GGACTTACAA AAGAAGAAAT CATCAAGCTT 
ATTTTTGAAA TATGTGGTTG TGGGCGTAAA TGCAAAGAGA CTCCGCTGGA GCTGCTGTTT 
GTGATTGACA GCTCAGAAAG CGTGGGCCTC AAGAACTTCC AAATCATCAA GAATTTTGTG 
AAGACCCTGA CAGACCGTGT CGCCCTGGAC CTGGCCACAA CCCGCGTAGG CATAATCAAC 
TACAGCCATA AGGTGGAGGA GGTGGCGCAC CTCACCCAGT TCTCCAGCAA GGATGACCTC 
AAGCGGGCTG TGGACAACAT GCAGTATCTG GGGGAAGGGA CCTACACGGC CACAGCGCTC 
CATGCCGCCA ACCGTATGTT TGAAGCTGCC AAGCCCGGCG TGAAGAAGGT GGCCTTGGTC 
ATCACTGATG GGCAGACAGA TACCCGAGAT GAGAAGAATC TGACTGAGGT GGTGAAGAAC 
GCCAGTGACA TCAATGTGGA AATATTTGTG ATTGGGGTAG TGAAGAGGAA TGACCCCAAC 
TTCCACATTT TCCACCAAGA AATGAATCTG ATTGCTACTG ACCCAGATAG TGAGCACGTT 
TATCTATTTG ATGACTTCAT CACTCTTGAA GATACCCTGA AGCAAAAATT ATTTACAAAA 
AGTTGTGAGG ATTTCGAAGC CTACCTCTTT CATATTTTGG GTTCCTCACC GCTTCAACCT 
GGATTTGGGA TATCGGGGGA AGAACGCCAT GAATCCACTC CAGAGCCCCA GAAAGAAATT 
TCTGAGTCTG TGCATGTCTC CAGGGCCCAG GACAGAGAGA ATGAGCCTTC AGAGAGGACC 
TGGGCTGCCA GCCCGGCCAC CACTCCTTCA CCTGAGGCTG CCACCATCCC GGGGCCACTG 
CCCAGGCCTC CTGAGGATCA GGCAAAGGGC CTGGAAACCA GAGCTCCGAG TTCCACTTCG 
AACTTAGAGC CAGAAAATTT TGTGCACAAA GATCCTAGAT GTCTGGAACC CCTACAACCA 
GGGAACTGTA GTGAATATGT GGTTCGATGG TATTATGACA AACAGGTCAA TTCCTGCGCT 
CGCTTTTGGT TCAGTGGCTG TAAAGGCTCG GGAAATAGAT TCAACAGCGA AAAGGAGTGT 
GAAGAAATCT GTATTCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846755.1
CDS	196..3639
Translation	MWNRRFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFIVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGMKVAVLMTDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPRGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGEKGSQGAIGPQGEAGPSIKGDKGDMGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKGDEGKKGSKGNQGQKGSQGPGGPKGDPGIMGPVGMPGISIPGPPGPKGDRGGPGMPGFKGEPGIAIRGPKGAQGPQGPVGAPGLKGDGYPGVPGPRGIPGPPGSRGLPGVGDTGAKGDPGVRGPPGPPGPRGIGTPGPKGDIGQKGLPGPPGSPGYGLQGIKGEQGPQGFPGPKGMTGHGLPGQKGEHGERGDVGKKGDKGEIGDPGPQGEQGLQGPKGDQGLTKEEIIKLIFEICGCGRKCKETPLELLFVIDSSESVGLKNFQIIKNFVKTLTDRVALDLATTRVGIINYSHKVEEVAHLTQFSSKDDLKRAVDNMQYLGEGTYTATALHAANRMFEAAKPGVKKVALVITDGQTDTRDEKNLTEVVKNASDINVEIFVIGVVKRNDPNFHIFHQEMNLIATDPDSEHVYLFDDFITLEDTLKQKLFTKSCEDFEAYLFHILGSSPLQPGFGISGEERHESTPEPQKEISESVHVSRAQDRENEPSERTWAASPATTPSPEAATIPGPLPRPPEDQAKGLETRAPSSTSNLEPENFVHKDPRCLEPLQPGNCSEYVVRWYYDKQVNSCARFWFSGCKGSGNRFNSEKECEEICIQG

Target ORF information:

RefSeq Version	XM_024990987.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990987.1

ORF Insert Sequence:

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCCGGA GGGCCAAAGG GTGATCCAGG AATTATGGGC 
CCTGTTGGGA TGCCTGGAAT ATCAATTCCT GGACCACCTG GACCAAAAGG AGACAGAGGT 
GGACCTGGGA TGCCTGGATT TAAAGGAGAA CCTGGAATTG CTATTCGAGG ACCAAAGGGT 
GCCCAAGGCC CGCAGGGACC AGTGGGTGCT CCAGGACTCA AAGGTGACGG CTATCCTGGT 
GTGCCCGGCC CTCGAGGAAT CCCAGGACCT CCTGGATCAA GGGGATTACC TGGAGTTGGA 
GACACTGGAG CAAAGGGAGA TCCTGGCGTC AGAGGCCCTC CAGGCCCCCC TGGGCCTCGG 
GGCATAGGAA CCCCAGGGCC AAAGGGTGAC ATCGGGCAGA AAGGCTTGCC TGGCCCTCCT 
GGCTCCCCTG GCTATGGATT ACAAGGAATC AAAGGGGAGC AAGGACCTCA GGGCTTCCCA 
GGGCCAAAGG GCATGACAGG CCATGGCCTC CCTGGCCAGA AGGGAGAGCA CGGAGAACGG 
GGCGATGTGG GAAAGAAAGG TGATAAAGGG GAAATTGGAG ATCCCGGACC CCAAGGAGAA 
CAGGGCTTAC AAGGACCAAA AGGAGACCAA GGACTTACAA AAGAAGAAAT CATCAAGCTT 
ATTTTTGAAA TATGTGGTTG TGGGCGTAAA TGCAAAGAGA CTCCGCTGGA GCTGCTGTTT 
GTGATTGACA GCTCAGAAAG CGTGGGCCTC AAGAACTTCC AAATCATCAA GAATTTTGTG 
AAGACCCTGA CAGACCGTGT CGCCCTGGAC CTGGCCACAA CCCGCGTAGG CATAATCAAC 
TACAGCCATA AGGTGGAGGA GGTGGCGCAC CTCACCCAGT TCTCCAGCAA GGATGACCTC 
AAGCGGGCTG TGGACAACAT GCAGTATCTG GGGGAAGGGA CCTACACGGC CACAGCGCTC 
CATGCCGCCA ACCGTATGTT TGAAGCTGCC AAGCCCGGCG TGAAGAAGGT GGCCTTGGTC 
ATCACTGATG GGCAGACAGA TACCCGAGAT GAGAAGAATC TGACTGAGGT GGTGAAGAAC 
GCCAGTGACA TCAATGTGGA AATATTTGTG ATTGGGGTAG TGAAGAGGAA TGACCCCAAC 
TTCCACATTT TCCACCAAGA AATGAATCTG ATTGCTACTG ACCCAGATAG TGAGCACGTT 
TATCTATTTG ATGACTTCAT CACTCTTGAA GATACCCTGA AGCAAAAATT ATTTACAAAA 
AGTTGTGAGG ATTTCGAAGC CTACCTCTTT CATATTTTGG GTTCCTCACC GCTTCAACCT 
GGATTTGGGA TATCGGGGGA AGAACGCCAT GAATCCACTC CAGAGCCCCA GAAAGAAATT 
TCTGAGTCTG TGCATGTCTC CAGGGCCCAG GACAGAGAGA ATGAGCCTTC AGAGAGGACC 
TGGGCTGCCA GCCCGGCCAC CACTCCTTCA CCTGAGGCTG CCACCATCCC GGGGCCACTG 
CCCAGGCCTC CTGAGGATCA GGCAAAGGGC CTGGAAACCA GAGCTCCGAG TTCCACTTCG 
AACTTAGAGC CAGAAAATTT TGTGCACAAA GATCCTAGAT GTCTGGAACC CCTACAACCA 
GGGAACTGTA GTGAATATGT GGTTCGATGG TATTATGACA AACAGGTCAA TTCCTGCGCT 
CGCTTTTGGT TCAGTGGCTG TAAAGGCTCG GGAAATAGAT TCAACAGCGA AAAGGAGTGT 
GAAGAAATCT GTATTCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100847952 ( XM_024990988.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846756.1 Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X2, mRNA.

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CloneID	OBa233910
Clone ID Related Accession (Same CDS sequence)	XM_024990988.1
Accession Version	XM_024990988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXVIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGGGATATGG GACCTGTGGG ACCCCAAGGA 
CTGATGGGCA TCCCTGGAAT TGGGAGTCAA GGGGAACAGG GAATCCAAGG TCCCATTGGC 
CCACCTGGTC CACAAGGGCC CCCAGGACAA GGCTCACCTG GTCCCAAGGG AGAAGTTGGC 
CAGATGGGCC CTACAGGCCC TAGAGGACCA GTGGGGCTCG GAGTACAAGG TCCAAAGGGG 
GAGCCAGGCT CAATAGGGCT CCCAGGACAG CCAGGAGTAC CAGGAGAAGA TGGAGCTGCA 
GGGAAGAAGG GAGAAGCAGG GCTTCCGGGG GCAAGAGGCC CTGAAGGACC ACCTGGGAAA 
GGACAGCCTG GCCTCAAGGG AGATGAAGGA AAGAAAGGGA GCAAAGGAAA TCAAGGACAG 
AAAGGGTCTC AGGGGCCCGG AGGGCCAAAG GGTGATCCAG GAATTATGGG CCCTGTTGGG 
ATGCCTGGAA TATCAATTCC TGGACCACCT GGACCAAAAG GAGACAGAGG TGGACCTGGG 
ATGCCTGGAT TTAAAGGAGA ACCTGGAATT GCTATTCGAG GACCAAAGGG TGCCCAAGGC 
CCGCAGGGAC CAGTGGGTGC TCCAGGACTC AAAGGTGACG GCTATCCTGG TGTGCCCGGC 
CCTCGAGGAA TCCCAGGACC TCCTGGATCA AGGGGATTAC CTGGAGTTGG AGACACTGGA 
GCAAAGGGAG ATCCTGGCGT CAGAGGCCCT CCAGGCCCCC CTGGGCCTCG GGGCATAGGA 
ACCCCAGGGC CAAAGGGTGA CATCGGGCAG AAAGGCTTGC CTGGCCCTCC TGGCTCCCCT 
GGCTATGGAT TACAAGGAAT CAAAGGGGAG CAAGGACCTC AGGGCTTCCC AGGGCCAAAG 
GGCATGACAG GCCATGGCCT CCCTGGCCAG AAGGGAGAGC ACGGAGAACG GGGCGATGTG 
GGAAAGAAAG GTGATAAAGG GGAAATTGGA GATCCCGGAC CCCAAGGAGA ACAGGGCTTA 
CAAGGACCAA AAGGAGACCA AGGACTTACA AAAGAAGAAA TCATCAAGCT TATTTTTGAA 
ATATGTGGTT GTGGGCGTAA ATGCAAAGAG ACTCCGCTGG AGCTGCTGTT TGTGATTGAC 
AGCTCAGAAA GCGTGGGCCT CAAGAACTTC CAAATCATCA AGAATTTTGT GAAGACCCTG 
ACAGACCGTG TCGCCCTGGA CCTGGCCACA ACCCGCGTAG GCATAATCAA CTACAGCCAT 
AAGGTGGAGG AGGTGGCGCA CCTCACCCAG TTCTCCAGCA AGGATGACCT CAAGCGGGCT 
GTGGACAACA TGCAGTATCT GGGGGAAGGG ACCTACACGG CCACAGCGCT CCATGCCGCC 
AACCGTATGT TTGAAGCTGC CAAGCCCGGC GTGAAGAAGG TGGCCTTGGT CATCACTGAT 
GGGCAGACAG ATACCCGAGA TGAGAAGAAT CTGACTGAGG TGGTGAAGAA CGCCAGTGAC 
ATCAATGTGG AAATATTTGT GATTGGGGTA GTGAAGAGGA ATGACCCCAA CTTCCACATT 
TTCCACCAAG AAATGAATCT GATTGCTACT GACCCAGATA GTGAGCACGT TTATCTATTT 
GATGACTTCA TCACTCTTGA AGATACCCTG AAGCAAAAAT TATTTACAAA AAGTTGTGAG 
GATTTCGAAG CCTACCTCTT TCATATTTTG GGTTCCTCAC CGCTTCAACC TGGATTTGGG 
ATATCGGGGG AAGAACGCCA TGAATCCACT CCAGAGCCCC AGAAAGAAAT TTCTGAGTCT 
GTGCATGTCT CCAGGGCCCA GGACAGAGAG AATGAGCCTT CAGAGAGGAC CTGGGCTGCC 
AGCCCGGCCA CCACTCCTTC ACCTGAGGCT GCCACCATCC CGGGGCCACT GCCCAGGCCT 
CCTGAGGATC AGGCAAAGGG CCTGGAAACC AGAGCTCCGA GTTCCACTTC GAACTTAGAG 
CCAGAAAATT TTGTGCACAA AGATCCTAGA TGTCTGGAAC CCCTACAACC AGGGAACTGT 
AGTGAATATG TGGTTCGATG GTATTATGAC AAACAGGTCA ATTCCTGCGC TCGCTTTTGG 
TTCAGTGGCT GTAAAGGCTC GGGAAATAGA TTCAACAGCG AAAAGGAGTG TGAAGAAATC 
TGTATTCAAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846756.1
CDS	196..3570
Translation	MWNRRFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFIVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGMKVAVLMTDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPRGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGDMGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKGDEGKKGSKGNQGQKGSQGPGGPKGDPGIMGPVGMPGISIPGPPGPKGDRGGPGMPGFKGEPGIAIRGPKGAQGPQGPVGAPGLKGDGYPGVPGPRGIPGPPGSRGLPGVGDTGAKGDPGVRGPPGPPGPRGIGTPGPKGDIGQKGLPGPPGSPGYGLQGIKGEQGPQGFPGPKGMTGHGLPGQKGEHGERGDVGKKGDKGEIGDPGPQGEQGLQGPKGDQGLTKEEIIKLIFEICGCGRKCKETPLELLFVIDSSESVGLKNFQIIKNFVKTLTDRVALDLATTRVGIINYSHKVEEVAHLTQFSSKDDLKRAVDNMQYLGEGTYTATALHAANRMFEAAKPGVKKVALVITDGQTDTRDEKNLTEVVKNASDINVEIFVIGVVKRNDPNFHIFHQEMNLIATDPDSEHVYLFDDFITLEDTLKQKLFTKSCEDFEAYLFHILGSSPLQPGFGISGEERHESTPEPQKEISESVHVSRAQDRENEPSERTWAASPATTPSPEAATIPGPLPRPPEDQAKGLETRAPSSTSNLEPENFVHKDPRCLEPLQPGNCSEYVVRWYYDKQVNSCARFWFSGCKGSGNRFNSEKECEEICIQG

Target ORF information:

RefSeq Version	XM_024990988.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990988.1

ORF Insert Sequence:

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGGGATATGG GACCTGTGGG ACCCCAAGGA 
CTGATGGGCA TCCCTGGAAT TGGGAGTCAA GGGGAACAGG GAATCCAAGG TCCCATTGGC 
CCACCTGGTC CACAAGGGCC CCCAGGACAA GGCTCACCTG GTCCCAAGGG AGAAGTTGGC 
CAGATGGGCC CTACAGGCCC TAGAGGACCA GTGGGGCTCG GAGTACAAGG TCCAAAGGGG 
GAGCCAGGCT CAATAGGGCT CCCAGGACAG CCAGGAGTAC CAGGAGAAGA TGGAGCTGCA 
GGGAAGAAGG GAGAAGCAGG GCTTCCGGGG GCAAGAGGCC CTGAAGGACC ACCTGGGAAA 
GGACAGCCTG GCCTCAAGGG AGATGAAGGA AAGAAAGGGA GCAAAGGAAA TCAAGGACAG 
AAAGGGTCTC AGGGGCCCGG AGGGCCAAAG GGTGATCCAG GAATTATGGG CCCTGTTGGG 
ATGCCTGGAA TATCAATTCC TGGACCACCT GGACCAAAAG GAGACAGAGG TGGACCTGGG 
ATGCCTGGAT TTAAAGGAGA ACCTGGAATT GCTATTCGAG GACCAAAGGG TGCCCAAGGC 
CCGCAGGGAC CAGTGGGTGC TCCAGGACTC AAAGGTGACG GCTATCCTGG TGTGCCCGGC 
CCTCGAGGAA TCCCAGGACC TCCTGGATCA AGGGGATTAC CTGGAGTTGG AGACACTGGA 
GCAAAGGGAG ATCCTGGCGT CAGAGGCCCT CCAGGCCCCC CTGGGCCTCG GGGCATAGGA 
ACCCCAGGGC CAAAGGGTGA CATCGGGCAG AAAGGCTTGC CTGGCCCTCC TGGCTCCCCT 
GGCTATGGAT TACAAGGAAT CAAAGGGGAG CAAGGACCTC AGGGCTTCCC AGGGCCAAAG 
GGCATGACAG GCCATGGCCT CCCTGGCCAG AAGGGAGAGC ACGGAGAACG GGGCGATGTG 
GGAAAGAAAG GTGATAAAGG GGAAATTGGA GATCCCGGAC CCCAAGGAGA ACAGGGCTTA 
CAAGGACCAA AAGGAGACCA AGGACTTACA AAAGAAGAAA TCATCAAGCT TATTTTTGAA 
ATATGTGGTT GTGGGCGTAA ATGCAAAGAG ACTCCGCTGG AGCTGCTGTT TGTGATTGAC 
AGCTCAGAAA GCGTGGGCCT CAAGAACTTC CAAATCATCA AGAATTTTGT GAAGACCCTG 
ACAGACCGTG TCGCCCTGGA CCTGGCCACA ACCCGCGTAG GCATAATCAA CTACAGCCAT 
AAGGTGGAGG AGGTGGCGCA CCTCACCCAG TTCTCCAGCA AGGATGACCT CAAGCGGGCT 
GTGGACAACA TGCAGTATCT GGGGGAAGGG ACCTACACGG CCACAGCGCT CCATGCCGCC 
AACCGTATGT TTGAAGCTGC CAAGCCCGGC GTGAAGAAGG TGGCCTTGGT CATCACTGAT 
GGGCAGACAG ATACCCGAGA TGAGAAGAAT CTGACTGAGG TGGTGAAGAA CGCCAGTGAC 
ATCAATGTGG AAATATTTGT GATTGGGGTA GTGAAGAGGA ATGACCCCAA CTTCCACATT 
TTCCACCAAG AAATGAATCT GATTGCTACT GACCCAGATA GTGAGCACGT TTATCTATTT 
GATGACTTCA TCACTCTTGA AGATACCCTG AAGCAAAAAT TATTTACAAA AAGTTGTGAG 
GATTTCGAAG CCTACCTCTT TCATATTTTG GGTTCCTCAC CGCTTCAACC TGGATTTGGG 
ATATCGGGGG AAGAACGCCA TGAATCCACT CCAGAGCCCC AGAAAGAAAT TTCTGAGTCT 
GTGCATGTCT CCAGGGCCCA GGACAGAGAG AATGAGCCTT CAGAGAGGAC CTGGGCTGCC 
AGCCCGGCCA CCACTCCTTC ACCTGAGGCT GCCACCATCC CGGGGCCACT GCCCAGGCCT 
CCTGAGGATC AGGCAAAGGG CCTGGAAACC AGAGCTCCGA GTTCCACTTC GAACTTAGAG 
CCAGAAAATT TTGTGCACAA AGATCCTAGA TGTCTGGAAC CCCTACAACC AGGGAACTGT 
AGTGAATATG TGGTTCGATG GTATTATGAC AAACAGGTCA ATTCCTGCGC TCGCTTTTGG 
TTCAGTGGCT GTAAAGGCTC GGGAAATAGA TTCAACAGCG AAAAGGAGTG TGAAGAAATC 
TGTATTCAAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100847952 ( XM_024990989.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846757.1 Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X3, mRNA.

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CloneID	OBa60405
Clone ID Related Accession (Same CDS sequence)	XM_024990989.1
Accession Version	XM_024990989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXVIII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCCGGA GGGCCAAAGG GTGCCCAAGG CCCGCAGGGA 
CCAGTGGGTG CTCCAGGACT CAAAGGTGAC GGCTATCCTG GTGTGCCCGG CCCTCGAGGA 
ATCCCAGGAC CTCCTGGATC AAGGGGATTA CCTGGAGTTG GAGACACTGG AGCAAAGGGA 
GATCCTGGCG TCAGAGGCCC TCCAGGCCCC CCTGGGCCTC GGGGCATAGG AACCCCAGGG 
CCAAAGGGTG ACATCGGGCA GAAAGGCTTG CCTGGCCCTC CTGGCTCCCC TGGCTATGGA 
TTACAAGGAA TCAAAGGGGA GCAAGGACCT CAGGGCTTCC CAGGGCCAAA GGGCATGACA 
GGCCATGGCC TCCCTGGCCA GAAGGGAGAG CACGGAGAAC GGGGCGATGT GGGAAAGAAA 
GGTGATAAAG GGGAAATTGG AGATCCCGGA CCCCAAGGAG AACAGGGCTT ACAAGGACCA 
AAAGGAGACC AAGGACTTAC AAAAGAAGAA ATCATCAAGC TTATTTTTGA AATATGTGGT 
TGTGGGCGTA AATGCAAAGA GACTCCGCTG GAGCTGCTGT TTGTGATTGA CAGCTCAGAA 
AGCGTGGGCC TCAAGAACTT CCAAATCATC AAGAATTTTG TGAAGACCCT GACAGACCGT 
GTCGCCCTGG ACCTGGCCAC AACCCGCGTA GGCATAATCA ACTACAGCCA TAAGGTGGAG 
GAGGTGGCGC ACCTCACCCA GTTCTCCAGC AAGGATGACC TCAAGCGGGC TGTGGACAAC 
ATGCAGTATC TGGGGGAAGG GACCTACACG GCCACAGCGC TCCATGCCGC CAACCGTATG 
TTTGAAGCTG CCAAGCCCGG CGTGAAGAAG GTGGCCTTGG TCATCACTGA TGGGCAGACA 
GATACCCGAG ATGAGAAGAA TCTGACTGAG GTGGTGAAGA ACGCCAGTGA CATCAATGTG 
GAAATATTTG TGATTGGGGT AGTGAAGAGG AATGACCCCA ACTTCCACAT TTTCCACCAA 
GAAATGAATC TGATTGCTAC TGACCCAGAT AGTGAGCACG TTTATCTATT TGATGACTTC 
ATCACTCTTG AAGATACCCT GAAGCAAAAA TTATTTACAA AAAGTTGTGA GGATTTCGAA 
GCCTACCTCT TTCATATTTT GGGTTCCTCA CCGCTTCAAC CTGGATTTGG GATATCGGGG 
GAAGAACGCC ATGAATCCAC TCCAGAGCCC CAGAAAGAAA TTTCTGAGTC TGTGCATGTC 
TCCAGGGCCC AGGACAGAGA GAATGAGCCT TCAGAGAGGA CCTGGGCTGC CAGCCCGGCC 
ACCACTCCTT CACCTGAGGC TGCCACCATC CCGGGGCCAC TGCCCAGGCC TCCTGAGGAT 
CAGGCAAAGG GCCTGGAAAC CAGAGCTCCG AGTTCCACTT CGAACTTAGA GCCAGAAAAT 
TTTGTGCACA AAGATCCTAG ATGTCTGGAA CCCCTACAAC CAGGGAACTG TAGTGAATAT 
GTGGTTCGAT GGTATTATGA CAAACAGGTC AATTCCTGCG CTCGCTTTTG GTTCAGTGGC 
TGTAAAGGCT CGGGAAATAG ATTCAACAGC GAAAAGGAGT GTGAAGAAAT CTGTATTCAA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846757.1
CDS	196..3501
Translation	MWNRRFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFIVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGMKVAVLMTDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPRGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGEKGSQGAIGPQGEAGPSIKGDKGDMGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKGDEGKKGSKGNQGQKGSQGPGGPKGAQGPQGPVGAPGLKGDGYPGVPGPRGIPGPPGSRGLPGVGDTGAKGDPGVRGPPGPPGPRGIGTPGPKGDIGQKGLPGPPGSPGYGLQGIKGEQGPQGFPGPKGMTGHGLPGQKGEHGERGDVGKKGDKGEIGDPGPQGEQGLQGPKGDQGLTKEEIIKLIFEICGCGRKCKETPLELLFVIDSSESVGLKNFQIIKNFVKTLTDRVALDLATTRVGIINYSHKVEEVAHLTQFSSKDDLKRAVDNMQYLGEGTYTATALHAANRMFEAAKPGVKKVALVITDGQTDTRDEKNLTEVVKNASDINVEIFVIGVVKRNDPNFHIFHQEMNLIATDPDSEHVYLFDDFITLEDTLKQKLFTKSCEDFEAYLFHILGSSPLQPGFGISGEERHESTPEPQKEISESVHVSRAQDRENEPSERTWAASPATTPSPEAATIPGPLPRPPEDQAKGLETRAPSSTSNLEPENFVHKDPRCLEPLQPGNCSEYVVRWYYDKQVNSCARFWFSGCKGSGNRFNSEKECEEICIQG

Target ORF information:

RefSeq Version	XM_024990989.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXVIII alpha 1 chain (COL28A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990989.1

ORF Insert Sequence:

ATGTGGAACA GACGTTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC ATCGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTGTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTACT ACGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCATGAAAG TGGCCGTGCT GATGACTGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGACGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ACGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAGCGCAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAC GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGCGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGACCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATCGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCCCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GACCTCCAGG ACCTACTGGT 
GTTGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGGCCAAAG GGTGAAAAAG GTTCTCAAGG AGCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATATGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TGGGGCTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCCGGA GGGCCAAAGG GTGCCCAAGG CCCGCAGGGA 
CCAGTGGGTG CTCCAGGACT CAAAGGTGAC GGCTATCCTG GTGTGCCCGG CCCTCGAGGA 
ATCCCAGGAC CTCCTGGATC AAGGGGATTA CCTGGAGTTG GAGACACTGG AGCAAAGGGA 
GATCCTGGCG TCAGAGGCCC TCCAGGCCCC CCTGGGCCTC GGGGCATAGG AACCCCAGGG 
CCAAAGGGTG ACATCGGGCA GAAAGGCTTG CCTGGCCCTC CTGGCTCCCC TGGCTATGGA 
TTACAAGGAA TCAAAGGGGA GCAAGGACCT CAGGGCTTCC CAGGGCCAAA GGGCATGACA 
GGCCATGGCC TCCCTGGCCA GAAGGGAGAG CACGGAGAAC GGGGCGATGT GGGAAAGAAA 
GGTGATAAAG GGGAAATTGG AGATCCCGGA CCCCAAGGAG AACAGGGCTT ACAAGGACCA 
AAAGGAGACC AAGGACTTAC AAAAGAAGAA ATCATCAAGC TTATTTTTGA AATATGTGGT 
TGTGGGCGTA AATGCAAAGA GACTCCGCTG GAGCTGCTGT TTGTGATTGA CAGCTCAGAA 
AGCGTGGGCC TCAAGAACTT CCAAATCATC AAGAATTTTG TGAAGACCCT GACAGACCGT 
GTCGCCCTGG ACCTGGCCAC AACCCGCGTA GGCATAATCA ACTACAGCCA TAAGGTGGAG 
GAGGTGGCGC ACCTCACCCA GTTCTCCAGC AAGGATGACC TCAAGCGGGC TGTGGACAAC 
ATGCAGTATC TGGGGGAAGG GACCTACACG GCCACAGCGC TCCATGCCGC CAACCGTATG 
TTTGAAGCTG CCAAGCCCGG CGTGAAGAAG GTGGCCTTGG TCATCACTGA TGGGCAGACA 
GATACCCGAG ATGAGAAGAA TCTGACTGAG GTGGTGAAGA ACGCCAGTGA CATCAATGTG 
GAAATATTTG TGATTGGGGT AGTGAAGAGG AATGACCCCA ACTTCCACAT TTTCCACCAA 
GAAATGAATC TGATTGCTAC TGACCCAGAT AGTGAGCACG TTTATCTATT TGATGACTTC 
ATCACTCTTG AAGATACCCT GAAGCAAAAA TTATTTACAA AAAGTTGTGA GGATTTCGAA 
GCCTACCTCT TTCATATTTT GGGTTCCTCA CCGCTTCAAC CTGGATTTGG GATATCGGGG 
GAAGAACGCC ATGAATCCAC TCCAGAGCCC CAGAAAGAAA TTTCTGAGTC TGTGCATGTC 
TCCAGGGCCC AGGACAGAGA GAATGAGCCT TCAGAGAGGA CCTGGGCTGC CAGCCCGGCC 
ACCACTCCTT CACCTGAGGC TGCCACCATC CCGGGGCCAC TGCCCAGGCC TCCTGAGGAT 
CAGGCAAAGG GCCTGGAAAC CAGAGCTCCG AGTTCCACTT CGAACTTAGA GCCAGAAAAT 
TTTGTGCACA AAGATCCTAG ATGTCTGGAA CCCCTACAAC CAGGGAACTG TAGTGAATAT 
GTGGTTCGAT GGTATTATGA CAAACAGGTC AATTCCTGCG CTCGCTTTTG GTTCAGTGGC 
TGTAAAGGCT CGGGAAATAG ATTCAACAGC GAAAAGGAGT GTGAAGAAAT CTGTATTCAA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100847952 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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