CILP cDNA ORF clone, Bos taurus(cattle)

The following CILP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CILP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa91616 XM_015464958.1
Latest version!
Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91616 XM_015473050.1
Latest version!
Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91617 XM_003582653.4
Latest version!
Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91617 XM_003586505.4
Latest version!
Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa219780 XM_015473050.2
Latest version!
Bos taurus cartilage intermediate layer protein (CILP), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa219781 XM_003586505.5
Latest version!
Bos taurus cartilage intermediate layer protein (CILP), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa219782 XM_024997906.1
Latest version!
Bos taurus cartilage intermediate layer protein (CILP), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa219783 XM_024997908.1
Latest version!
Bos taurus cartilage intermediate layer protein (CILP), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa91616
    Clone ID Related Accession (Same CDS sequence) XM_015464958.1 , XM_015473050.1
    Accession Version XM_015464958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC
    CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC
    CGGGTGTGCG CCCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC
    AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG
    CAGCGACCCG GGCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC
    TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT
    GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG
    TCACTGTGCA GTGAGGCCAC TGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC
    TGTGACCTTA CCTGCCCCAT GGGCAAGGCG AATGCTGAGT GTGACGCATG CATGTGTCAG
    GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT
    GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC
    CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT
    GCGCCTATCC ACCTCATGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG
    TTCGTGAGAG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT
    GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC ACAGGGAAGC CCAAACCAGA CAAGTATTTC
    TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG
    CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GAGCGATGCT
    GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC
    AACCCAAACC CTGAGAGCTA CCTTATCCGG CTGCCCCATG ACTGTTTCCA GAATGCCACC
    AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT
    AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAAGAC
    AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC
    CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGAG TCAGCGCAGC TGACAATGGG
    GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACCC GTGTGAGCAT GACCGGCTAC
    AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG
    GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT
    GCTGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCCACG
    GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG
    ATCCCATCCA AGAGTTTCTA CCGGCAGAAT GGGGAGCCCT ACATGGGAAA AGTAAAGGCC
    AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG
    AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG
    GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT
    GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAT
    CCAGACACAG GGCTCTGGGA GGAGGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC
    AGAAGGGAAG ACAGAACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT
    AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG
    AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTCT CTGTGATCAA CCTGGAGCCC
    AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT
    CCCAATGGGG CCTGCCTGCC TGCTTTCTGT GATGACCAGT CCCCCGATGC TTACTCTGCC
    TATGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC
    AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA
    GACCATGAGG ACCCACGGGT CAAGAAGACA GCTTTCCAGA TCAGCATGGC CAAGCCACGG
    CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT TCGGGAATGC
    GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGACCGGTAT
    GACTATAACA CAGTCCCATT CAACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320444.1
    CDS548..3397
    Translation

    Target ORF information:

    RefSeq Version XM_015464958.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC
    CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC
    CGGGTGTGCG CCCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC
    AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG
    CAGCGACCCG GGCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC
    TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT
    GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG
    TCACTGTGCA GTGAGGCCAC TGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC
    TGTGACCTTA CCTGCCCCAT GGGCAAGGCG AATGCTGAGT GTGACGCATG CATGTGTCAG
    GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT
    GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC
    CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT
    GCGCCTATCC ACCTCATGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG
    TTCGTGAGAG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT
    GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC ACAGGGAAGC CCAAACCAGA CAAGTATTTC
    TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG
    CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GAGCGATGCT
    GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC
    AACCCAAACC CTGAGAGCTA CCTTATCCGG CTGCCCCATG ACTGTTTCCA GAATGCCACC
    AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT
    AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAAGAC
    AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC
    CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGAG TCAGCGCAGC TGACAATGGG
    GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACCC GTGTGAGCAT GACCGGCTAC
    AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG
    GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT
    GCTGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCCACG
    GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG
    ATCCCATCCA AGAGTTTCTA CCGGCAGAAT GGGGAGCCCT ACATGGGAAA AGTAAAGGCC
    AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG
    AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG
    GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT
    GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAT
    CCAGACACAG GGCTCTGGGA GGAGGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC
    AGAAGGGAAG ACAGAACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT
    AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG
    AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTCT CTGTGATCAA CCTGGAGCCC
    AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT
    CCCAATGGGG CCTGCCTGCC TGCTTTCTGT GATGACCAGT CCCCCGATGC TTACTCTGCC
    TATGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC
    AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA
    GACCATGAGG ACCCACGGGT CAAGAAGACA GCTTTCCAGA TCAGCATGGC CAAGCCACGG
    CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT TCGGGAATGC
    GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGACCGGTAT
    GACTATAACA CAGTCCCATT CAACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91616
    Clone ID Related Accession (Same CDS sequence) XM_015464958.1 , XM_015473050.1
    Accession Version XM_015473050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC
    CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC
    CGGGTGTGCG CCCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC
    AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG
    CAGCGACCCG GGCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC
    TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT
    GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG
    TCACTGTGCA GTGAGGCCAC TGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC
    TGTGACCTTA CCTGCCCCAT GGGCAAGGCG AATGCTGAGT GTGACGCATG CATGTGTCAG
    GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT
    GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC
    CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT
    GCGCCTATCC ACCTCATGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG
    TTCGTGAGAG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT
    GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC ACAGGGAAGC CCAAACCAGA CAAGTATTTC
    TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG
    CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GAGCGATGCT
    GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC
    AACCCAAACC CTGAGAGCTA CCTTATCCGG CTGCCCCATG ACTGTTTCCA GAATGCCACC
    AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT
    AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAAGAC
    AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC
    CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGAG TCAGCGCAGC TGACAATGGG
    GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACCC GTGTGAGCAT GACCGGCTAC
    AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG
    GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT
    GCTGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCCACG
    GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG
    ATCCCATCCA AGAGTTTCTA CCGGCAGAAT GGGGAGCCCT ACATGGGAAA AGTAAAGGCC
    AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG
    AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG
    GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT
    GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAT
    CCAGACACAG GGCTCTGGGA GGAGGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC
    AGAAGGGAAG ACAGAACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT
    AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG
    AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTCT CTGTGATCAA CCTGGAGCCC
    AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT
    CCCAATGGGG CCTGCCTGCC TGCTTTCTGT GATGACCAGT CCCCCGATGC TTACTCTGCC
    TATGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC
    AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA
    GACCATGAGG ACCCACGGGT CAAGAAGACA GCTTTCCAGA TCAGCATGGC CAAGCCACGG
    CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT TCGGGAATGC
    GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGACCGGTAT
    GACTATAACA CAGTCCCATT CAACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328536.1
    CDS548..3397
    Translation

    Target ORF information:

    RefSeq Version XM_015473050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC
    CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC
    CGGGTGTGCG CCCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC
    AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG
    CAGCGACCCG GGCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC
    TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT
    GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG
    TCACTGTGCA GTGAGGCCAC TGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC
    TGTGACCTTA CCTGCCCCAT GGGCAAGGCG AATGCTGAGT GTGACGCATG CATGTGTCAG
    GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT
    GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC
    CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT
    GCGCCTATCC ACCTCATGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG
    TTCGTGAGAG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT
    GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC ACAGGGAAGC CCAAACCAGA CAAGTATTTC
    TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG
    CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GAGCGATGCT
    GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC
    AACCCAAACC CTGAGAGCTA CCTTATCCGG CTGCCCCATG ACTGTTTCCA GAATGCCACC
    AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT
    AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAAGAC
    AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC
    CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGAG TCAGCGCAGC TGACAATGGG
    GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACCC GTGTGAGCAT GACCGGCTAC
    AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG
    GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT
    GCTGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCCACG
    GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG
    ATCCCATCCA AGAGTTTCTA CCGGCAGAAT GGGGAGCCCT ACATGGGAAA AGTAAAGGCC
    AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG
    AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG
    GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT
    GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAT
    CCAGACACAG GGCTCTGGGA GGAGGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC
    AGAAGGGAAG ACAGAACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT
    AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG
    AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTCT CTGTGATCAA CCTGGAGCCC
    AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT
    CCCAATGGGG CCTGCCTGCC TGCTTTCTGT GATGACCAGT CCCCCGATGC TTACTCTGCC
    TATGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC
    AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA
    GACCATGAGG ACCCACGGGT CAAGAAGACA GCTTTCCAGA TCAGCATGGC CAAGCCACGG
    CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT TCGGGAATGC
    GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGACCGGTAT
    GACTATAACA CAGTCCCATT CAACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91617
    Clone ID Related Accession (Same CDS sequence) XM_003582653.4 , XM_003586505.4
    Accession Version XM_003582653.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582653.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGGAGGCAGA TGATGCTCAC TCAGTCAGTA AGGAGAGTCC AGCCTGGGAA GAGGACCTCA
    GGCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC
    AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC
    TATGGGGACC GGGTGTGCGC CCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA
    CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC
    AACAGGGAGC AGCGACCCGG GCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT
    CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC
    AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG
    GAGTCGGTGT CACTGTGCAG TGAGGCCACT GAGGAGGGCC GGCACTGCAT GGAACAGGCC
    TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCGA ATGCTGAGTG TGACGCATGC
    ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA
    GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT
    GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG
    ACCAAGTTTG CGCCTATCCA CCTCATGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC
    AGGGCGGAGT TCGTGAGAGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA
    CGAAGAGCTG GGCAGAGCGT GTCCCTGTGC TGTAAGGCCA CAGGGAAGCC CAAACCAGAC
    AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC
    AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG
    AGCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG
    ATTCCTTGCA ACCCAAACCC TGAGAGCTAC CTTATCCGGC TGCCCCATGA CTGTTTCCAG
    AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG
    AAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA
    ACAGAAGACA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG
    TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGAGT CAGCGCAGCT
    GACAATGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACCCG TGTGAGCATG
    ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC
    ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG
    AAGGGGAGTG CTGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG
    GAGGCCACGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT
    GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAATG GGGAGCCCTA CATGGGAAAA
    GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG
    AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG
    TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG
    GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG
    TCGCTCAATC CAGACACAGG GCTCTGGGAG GAGGAAGGTG ACTTCAAACC TGAAAGCCAA
    AGGCGGAACA GAAGGGAAGA CAGAACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG
    AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC
    AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTCTC TGTGATCAAC
    CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC
    CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCTTTCTGTG ATGACCAGTC CCCCGATGCT
    TACTCTGCCT ATGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT
    CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC
    CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACAG CTTTCCAGAT CAGCATGGCC
    AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTT
    CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG
    GACCGGTATG ACTATAACAC AGTCCCATTC AACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582701.3
    CDS287..3085
    Translation

    Target ORF information:

    RefSeq Version XM_003582653.4
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582653.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGGAGGCAGA TGATGCTCAC TCAGTCAGTA AGGAGAGTCC AGCCTGGGAA GAGGACCTCA
    GGCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC
    AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC
    TATGGGGACC GGGTGTGCGC CCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA
    CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC
    AACAGGGAGC AGCGACCCGG GCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT
    CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC
    AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG
    GAGTCGGTGT CACTGTGCAG TGAGGCCACT GAGGAGGGCC GGCACTGCAT GGAACAGGCC
    TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCGA ATGCTGAGTG TGACGCATGC
    ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA
    GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT
    GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG
    ACCAAGTTTG CGCCTATCCA CCTCATGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC
    AGGGCGGAGT TCGTGAGAGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA
    CGAAGAGCTG GGCAGAGCGT GTCCCTGTGC TGTAAGGCCA CAGGGAAGCC CAAACCAGAC
    AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC
    AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG
    AGCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG
    ATTCCTTGCA ACCCAAACCC TGAGAGCTAC CTTATCCGGC TGCCCCATGA CTGTTTCCAG
    AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG
    AAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA
    ACAGAAGACA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG
    TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGAGT CAGCGCAGCT
    GACAATGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACCCG TGTGAGCATG
    ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC
    ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG
    AAGGGGAGTG CTGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG
    GAGGCCACGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT
    GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAATG GGGAGCCCTA CATGGGAAAA
    GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG
    AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG
    TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG
    GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG
    TCGCTCAATC CAGACACAGG GCTCTGGGAG GAGGAAGGTG ACTTCAAACC TGAAAGCCAA
    AGGCGGAACA GAAGGGAAGA CAGAACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG
    AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC
    AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTCTC TGTGATCAAC
    CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC
    CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCTTTCTGTG ATGACCAGTC CCCCGATGCT
    TACTCTGCCT ATGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT
    CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC
    CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACAG CTTTCCAGAT CAGCATGGCC
    AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTT
    CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG
    GACCGGTATG ACTATAACAC AGTCCCATTC AACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91617
    Clone ID Related Accession (Same CDS sequence) XM_003582653.4 , XM_003586505.4
    Accession Version XM_003586505.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGGAGGCAGA TGATGCTCAC TCAGTCAGTA AGGAGAGTCC AGCCTGGGAA GAGGACCTCA
    GGCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC
    AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC
    TATGGGGACC GGGTGTGCGC CCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA
    CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC
    AACAGGGAGC AGCGACCCGG GCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT
    CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC
    AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG
    GAGTCGGTGT CACTGTGCAG TGAGGCCACT GAGGAGGGCC GGCACTGCAT GGAACAGGCC
    TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCGA ATGCTGAGTG TGACGCATGC
    ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA
    GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT
    GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG
    ACCAAGTTTG CGCCTATCCA CCTCATGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC
    AGGGCGGAGT TCGTGAGAGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA
    CGAAGAGCTG GGCAGAGCGT GTCCCTGTGC TGTAAGGCCA CAGGGAAGCC CAAACCAGAC
    AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC
    AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG
    AGCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG
    ATTCCTTGCA ACCCAAACCC TGAGAGCTAC CTTATCCGGC TGCCCCATGA CTGTTTCCAG
    AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG
    AAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA
    ACAGAAGACA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG
    TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGAGT CAGCGCAGCT
    GACAATGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACCCG TGTGAGCATG
    ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC
    ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG
    AAGGGGAGTG CTGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG
    GAGGCCACGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT
    GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAATG GGGAGCCCTA CATGGGAAAA
    GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG
    AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG
    TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG
    GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG
    TCGCTCAATC CAGACACAGG GCTCTGGGAG GAGGAAGGTG ACTTCAAACC TGAAAGCCAA
    AGGCGGAACA GAAGGGAAGA CAGAACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG
    AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC
    AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTCTC TGTGATCAAC
    CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC
    CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCTTTCTGTG ATGACCAGTC CCCCGATGCT
    TACTCTGCCT ATGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT
    CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC
    CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACAG CTTTCCAGAT CAGCATGGCC
    AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTT
    CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG
    GACCGGTATG ACTATAACAC AGTCCCATTC AACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586553.2
    CDS287..3085
    Translation

    Target ORF information:

    RefSeq Version XM_003586505.4
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein 1 (LOC100336614), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586505.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGGAGGCAGA TGATGCTCAC TCAGTCAGTA AGGAGAGTCC AGCCTGGGAA GAGGACCTCA
    GGCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC
    AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC
    TATGGGGACC GGGTGTGCGC CCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA
    CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC
    AACAGGGAGC AGCGACCCGG GCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT
    CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC
    AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG
    GAGTCGGTGT CACTGTGCAG TGAGGCCACT GAGGAGGGCC GGCACTGCAT GGAACAGGCC
    TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCGA ATGCTGAGTG TGACGCATGC
    ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA
    GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT
    GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG
    ACCAAGTTTG CGCCTATCCA CCTCATGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC
    AGGGCGGAGT TCGTGAGAGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA
    CGAAGAGCTG GGCAGAGCGT GTCCCTGTGC TGTAAGGCCA CAGGGAAGCC CAAACCAGAC
    AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC
    AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG
    AGCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG
    ATTCCTTGCA ACCCAAACCC TGAGAGCTAC CTTATCCGGC TGCCCCATGA CTGTTTCCAG
    AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG
    AAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA
    ACAGAAGACA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG
    TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGAGT CAGCGCAGCT
    GACAATGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACCCG TGTGAGCATG
    ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC
    ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG
    AAGGGGAGTG CTGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG
    GAGGCCACGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT
    GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAATG GGGAGCCCTA CATGGGAAAA
    GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG
    AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG
    TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG
    GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG
    TCGCTCAATC CAGACACAGG GCTCTGGGAG GAGGAAGGTG ACTTCAAACC TGAAAGCCAA
    AGGCGGAACA GAAGGGAAGA CAGAACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG
    AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC
    AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTCTC TGTGATCAAC
    CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC
    CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCTTTCTGTG ATGACCAGTC CCCCGATGCT
    TACTCTGCCT ATGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT
    CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC
    CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACAG CTTTCCAGAT CAGCATGGCC
    AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTT
    CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG
    GACCGGTATG ACTATAACAC AGTCCCATTC AACGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219780
    Clone ID Related Accession (Same CDS sequence) XM_015473050.2
    Accession Version XM_015473050.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC
    CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC
    CGGGTGTGCG CCCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC
    AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG
    CAGCGACCCG GGCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC
    TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT
    GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG
    TCACTGTGCA GTGAGGCCAC TGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC
    TGTGACCTTA CCTGCCCCAT GGGCAAGGCG AATGCTGAGT GTGACGCATG CATGTGTCAG
    GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT
    GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC
    CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT
    GCGCCTATCC ACCTCACGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG
    TTCGTGAGAG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT
    GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC ACAGGGAAGC CCAAACCAGA CAAGTATTTC
    TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG
    CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GAGCGATGCT
    GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC
    AACCCAAACC CTGAGAGCTA CCTTATCCGG CTGCCCCATG ACTGTTTCCA GAATGCCACC
    AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT
    AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAAGAC
    AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC
    CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGAG TCAGCGCAGC TGACAATGGG
    GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACCC GTGTGAGCAT GACCGGCTAC
    AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG
    GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT
    GCTGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCCACG
    GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG
    ATCCCATCCA AGAGTTTCTA CCGGCAGAAT GGGGAGCCCT ACATGGGAAA AGTAAAGGCC
    AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG
    AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG
    GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT
    GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAT
    CCAGACACAG GGCTCTGGGA GGAGGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC
    AGAAGGGAAG ACAGAACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT
    AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG
    AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTCT CTGTGATCAA CCTGGAGCCC
    AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT
    CCCAATGGGG CCTGCCTGCC TGCTTTCTGT GATGACCAGT CCCCCGATGC TTACTCTGCC
    TATGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC
    AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA
    GACCATGAGG ACCCACGGGT CAAGAAGACA GCTTTCCAGA TCAGCATGGC CAAGCCACGG
    CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT TCGGGAATGC
    GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGATCGGTAT
    GACTATAACA CAGTCCCTTT CAACGATGAT GACCCCATGG GCTGGACTGA AGACTACCTG
    GCATGGTGGC CCAAGCCAAT GGAGTTCAGG GCTTGCTACA TCAAGGTGAA GATTGTGGGG
    CCACTGGAGA TAAATGTGCG ATCCCGTAAC ATGGGGGGCA CCCACCGGCG GACAGTGGGA
    AAGCTGTACG GAATCCGGGA TGTGAAGAGC ACACGGGATA GGGACCAGCC CAACATCTCC
    TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG ATGCTCTATG ACCAGGACCG TGTGGACCGC
    ACACTGGTGA AGGTCATCCC CCAGGGCAGT TGCCAACGAG CCAGTGTAAA CTCTATGCTG
    CATGAGTACC TGGTCAACCA CCTACCACTG GCGGTCAACA ACAACACCAG TGAGTACACC
    ATGCTGGCGC CCTTGGACCC ACTGGGCCAC AACTATGGCA TCTACACCGT CACTGACCAG
    GACCCTCGCA CAGCCAAGGA GATTGCGCTC GGCCGGTGCT TTGATGGCAC ATCCGATGGC
    TCCTCCAGAG TCATGAAGGC CAATGTGGGA GTGGCCCTCA CCTTTAACTG CGTAGAGAGG
    CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC CTCCAGAACA CCCCGGCCCA GCCCTCCCCT
    GCAAGCACTG TCAGGGGAAG AGTGCCTTCA AGGAGGCAGC GGGCAAGTCA AGGTAGCCAG
    CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328536.2
    CDS548..4147
    Translation

    Target ORF information:

    RefSeq Version XM_015473050.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC
    CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC
    CGGGTGTGCG CCCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC
    AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG
    CAGCGACCCG GGCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC
    TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT
    GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG
    TCACTGTGCA GTGAGGCCAC TGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC
    TGTGACCTTA CCTGCCCCAT GGGCAAGGCG AATGCTGAGT GTGACGCATG CATGTGTCAG
    GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT
    GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC
    CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT
    GCGCCTATCC ACCTCACGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG
    TTCGTGAGAG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT
    GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC ACAGGGAAGC CCAAACCAGA CAAGTATTTC
    TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG
    CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GAGCGATGCT
    GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC
    AACCCAAACC CTGAGAGCTA CCTTATCCGG CTGCCCCATG ACTGTTTCCA GAATGCCACC
    AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT
    AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAAGAC
    AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC
    CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGAG TCAGCGCAGC TGACAATGGG
    GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACCC GTGTGAGCAT GACCGGCTAC
    AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG
    GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT
    GCTGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCCACG
    GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG
    ATCCCATCCA AGAGTTTCTA CCGGCAGAAT GGGGAGCCCT ACATGGGAAA AGTAAAGGCC
    AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG
    AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG
    GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT
    GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAT
    CCAGACACAG GGCTCTGGGA GGAGGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC
    AGAAGGGAAG ACAGAACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT
    AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG
    AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTCT CTGTGATCAA CCTGGAGCCC
    AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT
    CCCAATGGGG CCTGCCTGCC TGCTTTCTGT GATGACCAGT CCCCCGATGC TTACTCTGCC
    TATGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC
    AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA
    GACCATGAGG ACCCACGGGT CAAGAAGACA GCTTTCCAGA TCAGCATGGC CAAGCCACGG
    CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT TCGGGAATGC
    GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGATCGGTAT
    GACTATAACA CAGTCCCTTT CAACGATGAT GACCCCATGG GCTGGACTGA AGACTACCTG
    GCATGGTGGC CCAAGCCAAT GGAGTTCAGG GCTTGCTACA TCAAGGTGAA GATTGTGGGG
    CCACTGGAGA TAAATGTGCG ATCCCGTAAC ATGGGGGGCA CCCACCGGCG GACAGTGGGA
    AAGCTGTACG GAATCCGGGA TGTGAAGAGC ACACGGGATA GGGACCAGCC CAACATCTCC
    TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG ATGCTCTATG ACCAGGACCG TGTGGACCGC
    ACACTGGTGA AGGTCATCCC CCAGGGCAGT TGCCAACGAG CCAGTGTAAA CTCTATGCTG
    CATGAGTACC TGGTCAACCA CCTACCACTG GCGGTCAACA ACAACACCAG TGAGTACACC
    ATGCTGGCGC CCTTGGACCC ACTGGGCCAC AACTATGGCA TCTACACCGT CACTGACCAG
    GACCCTCGCA CAGCCAAGGA GATTGCGCTC GGCCGGTGCT TTGATGGCAC ATCCGATGGC
    TCCTCCAGAG TCATGAAGGC CAATGTGGGA GTGGCCCTCA CCTTTAACTG CGTAGAGAGG
    CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC CTCCAGAACA CCCCGGCCCA GCCCTCCCCT
    GCAAGCACTG TCAGGGGAAG AGTGCCTTCA AGGAGGCAGC GGGCAAGTCA AGGTAGCCAG
    CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219781
    Clone ID Related Accession (Same CDS sequence) XM_003586505.5
    Accession Version XM_003586505.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586505.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGGAGGCAGA TGATGCTCAC TCAGTCAGTA AGGAGAGTCC AGCCTGGGAA GAGGACCTCA
    GGCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC
    AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC
    TATGGGGACC GGGTGTGCGC CCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA
    CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC
    AACAGGGAGC AGCGACCCGG GCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT
    CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC
    AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG
    GAGTCGGTGT CACTGTGCAG TGAGGCCACT GAGGAGGGCC GGCACTGCAT GGAACAGGCC
    TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCGA ATGCTGAGTG TGACGCATGC
    ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA
    GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT
    GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG
    ACCAAGTTTG CGCCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC
    AGGGCGGAGT TCGTGAGAGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA
    CGAAGAGCTG GGCAGAGCGT GTCCCTGTGC TGTAAGGCCA CAGGGAAGCC CAAACCAGAC
    AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC
    AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG
    AGCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG
    ATTCCTTGCA ACCCAAACCC TGAGAGCTAC CTTATCCGGC TGCCCCATGA CTGTTTCCAG
    AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG
    AAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA
    ACAGAAGACA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG
    TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGAGT CAGCGCAGCT
    GACAATGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACCCG TGTGAGCATG
    ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC
    ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG
    AAGGGGAGTG CTGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG
    GAGGCCACGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT
    GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAATG GGGAGCCCTA CATGGGAAAA
    GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG
    AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG
    TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG
    GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG
    TCGCTCAATC CAGACACAGG GCTCTGGGAG GAGGAAGGTG ACTTCAAACC TGAAAGCCAA
    AGGCGGAACA GAAGGGAAGA CAGAACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG
    AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC
    AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTCTC TGTGATCAAC
    CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC
    CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCTTTCTGTG ATGACCAGTC CCCCGATGCT
    TACTCTGCCT ATGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT
    CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC
    CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACAG CTTTCCAGAT CAGCATGGCC
    AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTT
    CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG
    GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA
    GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG
    ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG
    ACAGTGGGAA AGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGATAG GGACCAGCCC
    AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT
    GTGGACCGCA CACTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC
    TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCACTGG CGGTCAACAA CAACACCAGT
    GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACCGTC
    ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA
    TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC
    GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG
    CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCTTCAA GGAGGCAGCG GGCAAGTCAA
    GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG
    AGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586553.3
    CDS265..3813
    Translation

    Target ORF information:

    RefSeq Version XM_003586505.5
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586505.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGGAGGCAGA TGATGCTCAC TCAGTCAGTA AGGAGAGTCC AGCCTGGGAA GAGGACCTCA
    GGCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC
    AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC
    TATGGGGACC GGGTGTGCGC CCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA
    CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC
    AACAGGGAGC AGCGACCCGG GCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT
    CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC
    AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG
    GAGTCGGTGT CACTGTGCAG TGAGGCCACT GAGGAGGGCC GGCACTGCAT GGAACAGGCC
    TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCGA ATGCTGAGTG TGACGCATGC
    ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA
    GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT
    GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG
    ACCAAGTTTG CGCCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC
    AGGGCGGAGT TCGTGAGAGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA
    CGAAGAGCTG GGCAGAGCGT GTCCCTGTGC TGTAAGGCCA CAGGGAAGCC CAAACCAGAC
    AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC
    AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG
    AGCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG
    ATTCCTTGCA ACCCAAACCC TGAGAGCTAC CTTATCCGGC TGCCCCATGA CTGTTTCCAG
    AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG
    AAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA
    ACAGAAGACA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG
    TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGAGT CAGCGCAGCT
    GACAATGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACCCG TGTGAGCATG
    ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC
    ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG
    AAGGGGAGTG CTGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG
    GAGGCCACGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT
    GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAATG GGGAGCCCTA CATGGGAAAA
    GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG
    AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG
    TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG
    GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG
    TCGCTCAATC CAGACACAGG GCTCTGGGAG GAGGAAGGTG ACTTCAAACC TGAAAGCCAA
    AGGCGGAACA GAAGGGAAGA CAGAACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG
    AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC
    AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTCTC TGTGATCAAC
    CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC
    CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCTTTCTGTG ATGACCAGTC CCCCGATGCT
    TACTCTGCCT ATGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT
    CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC
    CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACAG CTTTCCAGAT CAGCATGGCC
    AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTT
    CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG
    GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA
    GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG
    ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG
    ACAGTGGGAA AGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGATAG GGACCAGCCC
    AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT
    GTGGACCGCA CACTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC
    TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCACTGG CGGTCAACAA CAACACCAGT
    GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACCGTC
    ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA
    TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC
    GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG
    CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCTTCAA GGAGGCAGCG GGCAAGTCAA
    GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG
    AGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219782
    Clone ID Related Accession (Same CDS sequence) XM_024997906.1
    Accession Version XM_024997906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGATCC TTGCGCCGAG ACACAGAGCA CAGCCTCTGG
    AGTCCATGGT CTCCCTGGAG CAAGTGTTCT GCTGCTTGTG GTCACCCTGG GGTCCAGACC
    CGAACACGCA CCTGCTTGGC GGAGTCGGTG TCACTGTGCA GTGAGGCCAC TGAGGAGGGC
    CGGCACTGCA TGGAACAGGC CTGTTCAGCC TGTGACCTTA CCTGCCCCAT GGGCAAGGCG
    AATGCTGAGT GTGACGCATG CATGTGTCAG GACTTCGTGC TGTATGGGGC TGTCTCCCTC
    CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT GTCTACCTCC TGAGCAAGAC ACCTAAGCTA
    CTGACCCAGA CGGACAGCAG TGGAAGGTTC CGAGTCCCTG GGTTGTGCCC CGATGGCAAA
    AGCATCCTGA AGATCACAAA GACCAAGTTT GCGCCTATCC ACCTCACGGT GCCTAAGACT
    AGACTGAAGG CAGCCACCAT CAGGGCGGAG TTCGTGAGAG CAGAAACGCC GTACATTGTG
    ATGAGCCCTG AGACAAAAGC ACGAAGAGCT GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC
    ACAGGGAAGC CCAAACCAGA CAAGTATTTC TGGTACCATA ACAACACACT GTTGGACCCC
    TCCCTCTACA AGCACGACAG CAAGCTGGTG CTGAGGAATC TGCGGCGGGA CCAGGCCGGG
    GAGTACTTCT GCAAGGCCCA GAGCGATGCT GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG
    ACTGTCATAG CCTCTGATGA GATTCCTTGC AACCCAAACC CTGAGAGCTA CCTTATCCGG
    CTGCCCCATG ACTGTTTCCA GAATGCCACC AACTCCTTCT ACTATGATGT GGGTCGTTGC
    CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT AATGGGATCA GGTGCCGAGA TGCTGTGGAG
    CACTGCTGTG GGATCTCCAA AACAGAAGAC AGGGAGATCC AGTGCAGCGG GTACACGCTG
    CCCACCAAGG TGGCCACGGA GTGCAGCTGC CAGCGGTGTA CGGAGACTCG GAGAATCGTG
    CGGGGCCGAG TCAGCGCAGC TGACAATGGG GAACCCCTGC GCTTCGGCCA CGTGTACATG
    GGGAACACCC GTGTGAGCAT GACCGGCTAC AAGGGCACAT TCACTCTGCA TGTCCCTCAG
    GACACAGAGA GGCTGGTGCT CACGTTTGTG GACAGGATGC AGAAGTTCGT CAACACCACC
    AAAGTGCTGC CCTTCAATAA GAAGGGGAGT GCTGTGTTCC ATGAGATCAA GATGCTTCGG
    CGGAAAGAGC CCATCACCTT GGAGGCCACG GAGACCAACA TTATCCCCTT GGGGGATATG
    GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG ATCCCATCCA AGAGTTTCTA CCGGCAGAAT
    GGGGAGCCCT ACATGGGAAA AGTAAAGGCC AGTGTGACCT TCCTGGATCC CCGGAATATT
    TCCACAGCTA CTGCTGCCCA GAGTGACCTG AACTTCATCA ATGATGAAGG AGACACTTTC
    CCTCTTCGGA CATATGGCAT GTTCTCTGTG GACTTCAGAG ATGAAGCCAC CTCAGAGTCC
    CTTAATGTTG GCAAGGTGAA GGTTCATCTT GACTCGACCC AGGTCAAGAT GCCAGAGCAT
    GTGCCCATGA TGAAACTCTG GTCGCTCAAT CCAGACACAG GGCTCTGGGA GGAGGAAGGT
    GACTTCAAAC CTGAAAGCCA AAGGCGGAAC AGAAGGGAAG ACAGAACCTT CCTGGTGGGC
    AACATGGAGA TCCGTGAGAG GAGGCTTTTT AACCTGGATG TCCCGGAAAG CAGGAGGTGC
    TTCATCAAGG TGAGGGCCTA CAGGAGTGAG AGGTTCTTGC CCAGTGAGCA GATCCAGGGG
    GTTGTGGTCT CTGTGATCAA CCTGGAGCCC AGGACTGGCT TCTCCTCCAA CCCCAGGGCC
    TGGGGCCGCT TTGACAGTGT CCTCACGGGT CCCAATGGGG CCTGCCTGCC TGCTTTCTGT
    GATGACCAGT CCCCCGATGC TTACTCTGCC TATGTCTTGG CCAGCCTGGC TGGGGAAGAA
    CTGGAAGCAG TGGAGTCTTC TCCTAAATTC AACCCAAATG CAATTGGTGT CCCTCAGCCT
    TACCTCAACA AGCTCAAGTA CCGCCGGACA GACCATGAGG ACCCACGGGT CAAGAAGACA
    GCTTTCCAGA TCAGCATGGC CAAGCCACGG CCCAACTCAC CTGAGGAGGG CAATGGACCC
    ATCTATGCAT TTGAGAACCT TCGGGAATGC GAGGACGCAC CACCCAGTGC GGCCCATTTC
    CGGTTCTACC AGATTGAGGG GGATCGGTAT GACTATAACA CAGTCCCTTT CAACGATGAT
    GACCCCATGG GCTGGACTGA AGACTACCTG GCATGGTGGC CCAAGCCAAT GGAGTTCAGG
    GCTTGCTACA TCAAGGTGAA GATTGTGGGG CCACTGGAGA TAAATGTGCG ATCCCGTAAC
    ATGGGGGGCA CCCACCGGCG GACAGTGGGA AAGCTGTACG GAATCCGGGA TGTGAAGAGC
    ACACGGGATA GGGACCAGCC CAACATCTCC TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG
    ATGCTCTATG ACCAGGACCG TGTGGACCGC ACACTGGTGA AGGTCATCCC CCAGGGCAGT
    TGCCAACGAG CCAGTGTAAA CTCTATGCTG CATGAGTACC TGGTCAACCA CCTACCACTG
    GCGGTCAACA ACAACACCAG TGAGTACACC ATGCTGGCGC CCTTGGACCC ACTGGGCCAC
    AACTATGGCA TCTACACCGT CACTGACCAG GACCCTCGCA CAGCCAAGGA GATTGCGCTC
    GGCCGGTGCT TTGATGGCAC ATCCGATGGC TCCTCCAGAG TCATGAAGGC CAATGTGGGA
    GTGGCCCTCA CCTTTAACTG CGTAGAGAGG CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC
    CTCCAGAACA CCCCGGCCCA GCCCTCCCCT GCAAGCACTG TCAGGGGAAG AGTGCCTTCA
    AGGAGGCAGC GGGCAAGTCA AGGTAGCCAG CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT
    GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853674.1
    CDS138..3467
    Translation

    Target ORF information:

    RefSeq Version XM_024997906.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein (CILP), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGCATT CCCCACATCA CCCTGCTTCC CTACGTCCCG AGAGGCTAGT AGCAGGACCC 
    TGCTGGGCCC CTGTAACTGT CTTGGCTTCT CATTTCTCCT CCTGGCCCTC AGGGAGGCAG
    ATGATGCTCA CTCAGTCAGT AAGGAGAGTC CAGCCTGGGA AGAGGACCTC AGGCGTCTTT
    GCCAAGCCTG CTAACCTCCT GGACAGATCC TTGCGCCGAG ACACAGAGCA CAGCCTCTGG
    AGTCCATGGT CTCCCTGGAG CAAGTGTTCT GCTGCTTGTG GTCACCCTGG GGTCCAGACC
    CGAACACGCA CCTGCTTGGC GGAGTCGGTG TCACTGTGCA GTGAGGCCAC TGAGGAGGGC
    CGGCACTGCA TGGAACAGGC CTGTTCAGCC TGTGACCTTA CCTGCCCCAT GGGCAAGGCG
    AATGCTGAGT GTGACGCATG CATGTGTCAG GACTTCGTGC TGTATGGGGC TGTCTCCCTC
    CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT GTCTACCTCC TGAGCAAGAC ACCTAAGCTA
    CTGACCCAGA CGGACAGCAG TGGAAGGTTC CGAGTCCCTG GGTTGTGCCC CGATGGCAAA
    AGCATCCTGA AGATCACAAA GACCAAGTTT GCGCCTATCC ACCTCACGGT GCCTAAGACT
    AGACTGAAGG CAGCCACCAT CAGGGCGGAG TTCGTGAGAG CAGAAACGCC GTACATTGTG
    ATGAGCCCTG AGACAAAAGC ACGAAGAGCT GGGCAGAGCG TGTCCCTGTG CTGTAAGGCC
    ACAGGGAAGC CCAAACCAGA CAAGTATTTC TGGTACCATA ACAACACACT GTTGGACCCC
    TCCCTCTACA AGCACGACAG CAAGCTGGTG CTGAGGAATC TGCGGCGGGA CCAGGCCGGG
    GAGTACTTCT GCAAGGCCCA GAGCGATGCT GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG
    ACTGTCATAG CCTCTGATGA GATTCCTTGC AACCCAAACC CTGAGAGCTA CCTTATCCGG
    CTGCCCCATG ACTGTTTCCA GAATGCCACC AACTCCTTCT ACTATGATGT GGGTCGTTGC
    CCTGTCAAGA CCTGTGCAGG GAAGCAGGAT AATGGGATCA GGTGCCGAGA TGCTGTGGAG
    CACTGCTGTG GGATCTCCAA AACAGAAGAC AGGGAGATCC AGTGCAGCGG GTACACGCTG
    CCCACCAAGG TGGCCACGGA GTGCAGCTGC CAGCGGTGTA CGGAGACTCG GAGAATCGTG
    CGGGGCCGAG TCAGCGCAGC TGACAATGGG GAACCCCTGC GCTTCGGCCA CGTGTACATG
    GGGAACACCC GTGTGAGCAT GACCGGCTAC AAGGGCACAT TCACTCTGCA TGTCCCTCAG
    GACACAGAGA GGCTGGTGCT CACGTTTGTG GACAGGATGC AGAAGTTCGT CAACACCACC
    AAAGTGCTGC CCTTCAATAA GAAGGGGAGT GCTGTGTTCC ATGAGATCAA GATGCTTCGG
    CGGAAAGAGC CCATCACCTT GGAGGCCACG GAGACCAACA TTATCCCCTT GGGGGATATG
    GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG ATCCCATCCA AGAGTTTCTA CCGGCAGAAT
    GGGGAGCCCT ACATGGGAAA AGTAAAGGCC AGTGTGACCT TCCTGGATCC CCGGAATATT
    TCCACAGCTA CTGCTGCCCA GAGTGACCTG AACTTCATCA ATGATGAAGG AGACACTTTC
    CCTCTTCGGA CATATGGCAT GTTCTCTGTG GACTTCAGAG ATGAAGCCAC CTCAGAGTCC
    CTTAATGTTG GCAAGGTGAA GGTTCATCTT GACTCGACCC AGGTCAAGAT GCCAGAGCAT
    GTGCCCATGA TGAAACTCTG GTCGCTCAAT CCAGACACAG GGCTCTGGGA GGAGGAAGGT
    GACTTCAAAC CTGAAAGCCA AAGGCGGAAC AGAAGGGAAG ACAGAACCTT CCTGGTGGGC
    AACATGGAGA TCCGTGAGAG GAGGCTTTTT AACCTGGATG TCCCGGAAAG CAGGAGGTGC
    TTCATCAAGG TGAGGGCCTA CAGGAGTGAG AGGTTCTTGC CCAGTGAGCA GATCCAGGGG
    GTTGTGGTCT CTGTGATCAA CCTGGAGCCC AGGACTGGCT TCTCCTCCAA CCCCAGGGCC
    TGGGGCCGCT TTGACAGTGT CCTCACGGGT CCCAATGGGG CCTGCCTGCC TGCTTTCTGT
    GATGACCAGT CCCCCGATGC TTACTCTGCC TATGTCTTGG CCAGCCTGGC TGGGGAAGAA
    CTGGAAGCAG TGGAGTCTTC TCCTAAATTC AACCCAAATG CAATTGGTGT CCCTCAGCCT
    TACCTCAACA AGCTCAAGTA CCGCCGGACA GACCATGAGG ACCCACGGGT CAAGAAGACA
    GCTTTCCAGA TCAGCATGGC CAAGCCACGG CCCAACTCAC CTGAGGAGGG CAATGGACCC
    ATCTATGCAT TTGAGAACCT TCGGGAATGC GAGGACGCAC CACCCAGTGC GGCCCATTTC
    CGGTTCTACC AGATTGAGGG GGATCGGTAT GACTATAACA CAGTCCCTTT CAACGATGAT
    GACCCCATGG GCTGGACTGA AGACTACCTG GCATGGTGGC CCAAGCCAAT GGAGTTCAGG
    GCTTGCTACA TCAAGGTGAA GATTGTGGGG CCACTGGAGA TAAATGTGCG ATCCCGTAAC
    ATGGGGGGCA CCCACCGGCG GACAGTGGGA AAGCTGTACG GAATCCGGGA TGTGAAGAGC
    ACACGGGATA GGGACCAGCC CAACATCTCC TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG
    ATGCTCTATG ACCAGGACCG TGTGGACCGC ACACTGGTGA AGGTCATCCC CCAGGGCAGT
    TGCCAACGAG CCAGTGTAAA CTCTATGCTG CATGAGTACC TGGTCAACCA CCTACCACTG
    GCGGTCAACA ACAACACCAG TGAGTACACC ATGCTGGCGC CCTTGGACCC ACTGGGCCAC
    AACTATGGCA TCTACACCGT CACTGACCAG GACCCTCGCA CAGCCAAGGA GATTGCGCTC
    GGCCGGTGCT TTGATGGCAC ATCCGATGGC TCCTCCAGAG TCATGAAGGC CAATGTGGGA
    GTGGCCCTCA CCTTTAACTG CGTAGAGAGG CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC
    CTCCAGAACA CCCCGGCCCA GCCCTCCCCT GCAAGCACTG TCAGGGGAAG AGTGCCTTCA
    AGGAGGCAGC GGGCAAGTCA AGGTAGCCAG CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT
    GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219783
    Clone ID Related Accession (Same CDS sequence) XM_024997908.1
    Accession Version XM_024997908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cartilage intermediate layer protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGATCCTTGC GCCGAGACAC AGAGCACAGC CTCTGGAGTC CATGGTCTCC CTGGAGCAAG
    TGTTCTGCTG CTTGTGGTCA CCCTGGGGTC CAGACCCGAA CACGCACCTG CTTGGCGGAG
    TCGGTGTCAC TGTGCAGTGA GGCCACTGAG GAGGGCCGGC ACTGCATGGA ACAGGCCTGT
    TCAGCCTGTG ACCTTACCTG CCCCATGGGC AAGGCGAATG CTGAGTGTGA CGCATGCATG
    TGTCAGGACT TCGTGCTGTA TGGGGCTGTC TCCCTCCCTG GGGGTGCCCC AGCCTCAGGG
    GCTGCTGTCT ACCTCCTGAG CAAGACACCT AAGCTACTGA CCCAGACGGA CAGCAGTGGA
    AGGTTCCGAG TCCCTGGGTT GTGCCCCGAT GGCAAAAGCA TCCTGAAGAT CACAAAGACC
    AAGTTTGCGC CTATCCACCT CACGGTGCCT AAGACTAGAC TGAAGGCAGC CACCATCAGG
    GCGGAGTTCG TGAGAGCAGA AACGCCGTAC ATTGTGATGA GCCCTGAGAC AAAAGCACGA
    AGAGCTGGGC AGAGCGTGTC CCTGTGCTGT AAGGCCACAG GGAAGCCCAA ACCAGACAAG
    TATTTCTGGT ACCATAACAA CACACTGTTG GACCCCTCCC TCTACAAGCA CGACAGCAAG
    CTGGTGCTGA GGAATCTGCG GCGGGACCAG GCCGGGGAGT ACTTCTGCAA GGCCCAGAGC
    GATGCTGGGG CTGCAAAGTC CCGCATTGCC CAGCTGACTG TCATAGCCTC TGATGAGATT
    CCTTGCAACC CAAACCCTGA GAGCTACCTT ATCCGGCTGC CCCATGACTG TTTCCAGAAT
    GCCACCAACT CCTTCTACTA TGATGTGGGT CGTTGCCCTG TCAAGACCTG TGCAGGGAAG
    CAGGATAATG GGATCAGGTG CCGAGATGCT GTGGAGCACT GCTGTGGGAT CTCCAAAACA
    GAAGACAGGG AGATCCAGTG CAGCGGGTAC ACGCTGCCCA CCAAGGTGGC CACGGAGTGC
    AGCTGCCAGC GGTGTACGGA GACTCGGAGA ATCGTGCGGG GCCGAGTCAG CGCAGCTGAC
    AATGGGGAAC CCCTGCGCTT CGGCCACGTG TACATGGGGA ACACCCGTGT GAGCATGACC
    GGCTACAAGG GCACATTCAC TCTGCATGTC CCTCAGGACA CAGAGAGGCT GGTGCTCACG
    TTTGTGGACA GGATGCAGAA GTTCGTCAAC ACCACCAAAG TGCTGCCCTT CAATAAGAAG
    GGGAGTGCTG TGTTCCATGA GATCAAGATG CTTCGGCGGA AAGAGCCCAT CACCTTGGAG
    GCCACGGAGA CCAACATTAT CCCCTTGGGG GATATGGCTG GTGAAGACCC TGTGGCTGAG
    CTGGAGATCC CATCCAAGAG TTTCTACCGG CAGAATGGGG AGCCCTACAT GGGAAAAGTA
    AAGGCCAGTG TGACCTTCCT GGATCCCCGG AATATTTCCA CAGCTACTGC TGCCCAGAGT
    GACCTGAACT TCATCAATGA TGAAGGAGAC ACTTTCCCTC TTCGGACATA TGGCATGTTC
    TCTGTGGACT TCAGAGATGA AGCCACCTCA GAGTCCCTTA ATGTTGGCAA GGTGAAGGTT
    CATCTTGACT CGACCCAGGT CAAGATGCCA GAGCATGTGC CCATGATGAA ACTCTGGTCG
    CTCAATCCAG ACACAGGGCT CTGGGAGGAG GAAGGTGACT TCAAACCTGA AAGCCAAAGG
    CGGAACAGAA GGGAAGACAG AACCTTCCTG GTGGGCAACA TGGAGATCCG TGAGAGGAGG
    CTTTTTAACC TGGATGTCCC GGAAAGCAGG AGGTGCTTCA TCAAGGTGAG GGCCTACAGG
    AGTGAGAGGT TCTTGCCCAG TGAGCAGATC CAGGGGGTTG TGGTCTCTGT GATCAACCTG
    GAGCCCAGGA CTGGCTTCTC CTCCAACCCC AGGGCCTGGG GCCGCTTTGA CAGTGTCCTC
    ACGGGTCCCA ATGGGGCCTG CCTGCCTGCT TTCTGTGATG ACCAGTCCCC CGATGCTTAC
    TCTGCCTATG TCTTGGCCAG CCTGGCTGGG GAAGAACTGG AAGCAGTGGA GTCTTCTCCT
    AAATTCAACC CAAATGCAAT TGGTGTCCCT CAGCCTTACC TCAACAAGCT CAAGTACCGC
    CGGACAGACC ATGAGGACCC ACGGGTCAAG AAGACAGCTT TCCAGATCAG CATGGCCAAG
    CCACGGCCCA ACTCACCTGA GGAGGGCAAT GGACCCATCT ATGCATTTGA GAACCTTCGG
    GAATGCGAGG ACGCACCACC CAGTGCGGCC CATTTCCGGT TCTACCAGAT TGAGGGGGAT
    CGGTATGACT ATAACACAGT CCCTTTCAAC GATGATGACC CCATGGGCTG GACTGAAGAC
    TACCTGGCAT GGTGGCCCAA GCCAATGGAG TTCAGGGCTT GCTACATCAA GGTGAAGATT
    GTGGGGCCAC TGGAGATAAA TGTGCGATCC CGTAACATGG GGGGCACCCA CCGGCGGACA
    GTGGGAAAGC TGTACGGAAT CCGGGATGTG AAGAGCACAC GGGATAGGGA CCAGCCCAAC
    ATCTCCTCTG CCTGTTTGGA GTTCAAGTGC AGTGGGATGC TCTATGACCA GGACCGTGTG
    GACCGCACAC TGGTGAAGGT CATCCCCCAG GGCAGTTGCC AACGAGCCAG TGTAAACTCT
    ATGCTGCATG AGTACCTGGT CAACCACCTA CCACTGGCGG TCAACAACAA CACCAGTGAG
    TACACCATGC TGGCGCCCTT GGACCCACTG GGCCACAACT ATGGCATCTA CACCGTCACT
    GACCAGGACC CTCGCACAGC CAAGGAGATT GCGCTCGGCC GGTGCTTTGA TGGCACATCC
    GATGGCTCCT CCAGAGTCAT GAAGGCCAAT GTGGGAGTGG CCCTCACCTT TAACTGCGTA
    GAGAGGCAGG TGGGCCGTCA GAGTGCCTTC CAGTACCTCC AGAACACCCC GGCCCAGCCC
    TCCCCTGCAA GCACTGTCAG GGGAAGAGTG CCTTCAAGGA GGCAGCGGGC AAGTCAAGGT
    AGCCAGCGTG GAGGGGTGGC CTCTCTGAGG TTTCCTGGGG CTGCTCAGCA GCCTCTGAGC
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853676.1
    CDS264..3449
    Translation

    Target ORF information:

    RefSeq Version XM_024997908.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cartilage intermediate layer protein (CILP), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGCAGATCAC CTCAGCGTTG 
    GGATCCTTGC GCCGAGACAC AGAGCACAGC CTCTGGAGTC CATGGTCTCC CTGGAGCAAG
    TGTTCTGCTG CTTGTGGTCA CCCTGGGGTC CAGACCCGAA CACGCACCTG CTTGGCGGAG
    TCGGTGTCAC TGTGCAGTGA GGCCACTGAG GAGGGCCGGC ACTGCATGGA ACAGGCCTGT
    TCAGCCTGTG ACCTTACCTG CCCCATGGGC AAGGCGAATG CTGAGTGTGA CGCATGCATG
    TGTCAGGACT TCGTGCTGTA TGGGGCTGTC TCCCTCCCTG GGGGTGCCCC AGCCTCAGGG
    GCTGCTGTCT ACCTCCTGAG CAAGACACCT AAGCTACTGA CCCAGACGGA CAGCAGTGGA
    AGGTTCCGAG TCCCTGGGTT GTGCCCCGAT GGCAAAAGCA TCCTGAAGAT CACAAAGACC
    AAGTTTGCGC CTATCCACCT CACGGTGCCT AAGACTAGAC TGAAGGCAGC CACCATCAGG
    GCGGAGTTCG TGAGAGCAGA AACGCCGTAC ATTGTGATGA GCCCTGAGAC AAAAGCACGA
    AGAGCTGGGC AGAGCGTGTC CCTGTGCTGT AAGGCCACAG GGAAGCCCAA ACCAGACAAG
    TATTTCTGGT ACCATAACAA CACACTGTTG GACCCCTCCC TCTACAAGCA CGACAGCAAG
    CTGGTGCTGA GGAATCTGCG GCGGGACCAG GCCGGGGAGT ACTTCTGCAA GGCCCAGAGC
    GATGCTGGGG CTGCAAAGTC CCGCATTGCC CAGCTGACTG TCATAGCCTC TGATGAGATT
    CCTTGCAACC CAAACCCTGA GAGCTACCTT ATCCGGCTGC CCCATGACTG TTTCCAGAAT
    GCCACCAACT CCTTCTACTA TGATGTGGGT CGTTGCCCTG TCAAGACCTG TGCAGGGAAG
    CAGGATAATG GGATCAGGTG CCGAGATGCT GTGGAGCACT GCTGTGGGAT CTCCAAAACA
    GAAGACAGGG AGATCCAGTG CAGCGGGTAC ACGCTGCCCA CCAAGGTGGC CACGGAGTGC
    AGCTGCCAGC GGTGTACGGA GACTCGGAGA ATCGTGCGGG GCCGAGTCAG CGCAGCTGAC
    AATGGGGAAC CCCTGCGCTT CGGCCACGTG TACATGGGGA ACACCCGTGT GAGCATGACC
    GGCTACAAGG GCACATTCAC TCTGCATGTC CCTCAGGACA CAGAGAGGCT GGTGCTCACG
    TTTGTGGACA GGATGCAGAA GTTCGTCAAC ACCACCAAAG TGCTGCCCTT CAATAAGAAG
    GGGAGTGCTG TGTTCCATGA GATCAAGATG CTTCGGCGGA AAGAGCCCAT CACCTTGGAG
    GCCACGGAGA CCAACATTAT CCCCTTGGGG GATATGGCTG GTGAAGACCC TGTGGCTGAG
    CTGGAGATCC CATCCAAGAG TTTCTACCGG CAGAATGGGG AGCCCTACAT GGGAAAAGTA
    AAGGCCAGTG TGACCTTCCT GGATCCCCGG AATATTTCCA CAGCTACTGC TGCCCAGAGT
    GACCTGAACT TCATCAATGA TGAAGGAGAC ACTTTCCCTC TTCGGACATA TGGCATGTTC
    TCTGTGGACT TCAGAGATGA AGCCACCTCA GAGTCCCTTA ATGTTGGCAA GGTGAAGGTT
    CATCTTGACT CGACCCAGGT CAAGATGCCA GAGCATGTGC CCATGATGAA ACTCTGGTCG
    CTCAATCCAG ACACAGGGCT CTGGGAGGAG GAAGGTGACT TCAAACCTGA AAGCCAAAGG
    CGGAACAGAA GGGAAGACAG AACCTTCCTG GTGGGCAACA TGGAGATCCG TGAGAGGAGG
    CTTTTTAACC TGGATGTCCC GGAAAGCAGG AGGTGCTTCA TCAAGGTGAG GGCCTACAGG
    AGTGAGAGGT TCTTGCCCAG TGAGCAGATC CAGGGGGTTG TGGTCTCTGT GATCAACCTG
    GAGCCCAGGA CTGGCTTCTC CTCCAACCCC AGGGCCTGGG GCCGCTTTGA CAGTGTCCTC
    ACGGGTCCCA ATGGGGCCTG CCTGCCTGCT TTCTGTGATG ACCAGTCCCC CGATGCTTAC
    TCTGCCTATG TCTTGGCCAG CCTGGCTGGG GAAGAACTGG AAGCAGTGGA GTCTTCTCCT
    AAATTCAACC CAAATGCAAT TGGTGTCCCT CAGCCTTACC TCAACAAGCT CAAGTACCGC
    CGGACAGACC ATGAGGACCC ACGGGTCAAG AAGACAGCTT TCCAGATCAG CATGGCCAAG
    CCACGGCCCA ACTCACCTGA GGAGGGCAAT GGACCCATCT ATGCATTTGA GAACCTTCGG
    GAATGCGAGG ACGCACCACC CAGTGCGGCC CATTTCCGGT TCTACCAGAT TGAGGGGGAT
    CGGTATGACT ATAACACAGT CCCTTTCAAC GATGATGACC CCATGGGCTG GACTGAAGAC
    TACCTGGCAT GGTGGCCCAA GCCAATGGAG TTCAGGGCTT GCTACATCAA GGTGAAGATT
    GTGGGGCCAC TGGAGATAAA TGTGCGATCC CGTAACATGG GGGGCACCCA CCGGCGGACA
    GTGGGAAAGC TGTACGGAAT CCGGGATGTG AAGAGCACAC GGGATAGGGA CCAGCCCAAC
    ATCTCCTCTG CCTGTTTGGA GTTCAAGTGC AGTGGGATGC TCTATGACCA GGACCGTGTG
    GACCGCACAC TGGTGAAGGT CATCCCCCAG GGCAGTTGCC AACGAGCCAG TGTAAACTCT
    ATGCTGCATG AGTACCTGGT CAACCACCTA CCACTGGCGG TCAACAACAA CACCAGTGAG
    TACACCATGC TGGCGCCCTT GGACCCACTG GGCCACAACT ATGGCATCTA CACCGTCACT
    GACCAGGACC CTCGCACAGC CAAGGAGATT GCGCTCGGCC GGTGCTTTGA TGGCACATCC
    GATGGCTCCT CCAGAGTCAT GAAGGCCAAT GTGGGAGTGG CCCTCACCTT TAACTGCGTA
    GAGAGGCAGG TGGGCCGTCA GAGTGCCTTC CAGTACCTCC AGAACACCCC GGCCCAGCCC
    TCCCCTGCAA GCACTGTCAG GGGAAGAGTG CCTTCAAGGA GGCAGCGGGC AAGTCAAGGT
    AGCCAGCGTG GAGGGGTGGC CTCTCTGAGG TTTCCTGGGG CTGCTCAGCA GCCTCTGAGC
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.