CILP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CILP
Entrez Gene ID	8483
Full Name	cartilage intermediate layer protein
Synonyms	CILP-1,HsT18872
General protein information	Preferred Names cartilage intermediate layer protein Names cartilage intermediate layer protein 1 cartilage intermediate layer protein 1 C1 cartilage intermediate layer protein 1 C2 cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q22.31
Summary	Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010].
Disorder MIM:	603489
Disorder Html:	{Lumbar disc disease, susceptibility to}, 603932 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017022678.2	XP_016878167.1	cartilage intermediate layer protein 1 isoform X1
XM_017022679.1	XP_016878168.1	cartilage intermediate layer protein 1 isoform X2
NM_003613.4	NP_003604.4	cartilage intermediate layer protein 1 preproprotein
NM_003613.3	NP_003604.3	cartilage intermediate layer protein 1 preproprotein
XM_017022678.1	XP_016878167.1	cartilage intermediate layer protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CILP	NP_003604.3
	CILP	XP_001108811.1
Pan troglodytes (chimpanzee)	CILP	XP_510481.2
Rattus norvegicus (Norway rat)	Cilp	NP_001101631.1
Danio rerio (zebrafish)	cilp	NP_001186291.1
Bos taurus (cattle)	CILP	XP_003586553.1
Canis lupus familiaris (dog)	CILP	XP_544728.4
Mus musculus (house mouse)	Cilp	NP_775561.1
Gallus gallus (chicken)	CILP	XP_004943946.1
Xenopus tropicalis (tropical clawed frog)	cilp	XP_002938402.2

Gene Ontology
Bibliography

Related articles in PubMed

Functional polymorphisms in asporin and CILP together with joint loading predispose to hand osteoarthritis.
Taipale M, Solovieva S, Leino-Arjas P, M?nnikk? M
BMC genetics18(1)108(2017 Dec)

Cartilage intermediate layer protein 1 (CILP1): A novel mediator of cardiac extracellular matrix remodelling.
van Nieuwenhoven FA, Munts C, Op't Veld RC, Gonz?lez A, D?ez J, Heymans S, Schroen B, van Bilsen M
Scientific reports7(1)16042(2017 Nov)

Association Between Cartilage Intermediate Layer Protein and Degeneration of Intervertebral Disc: A Meta-analysis.
Wang W, Hao J, Zheng S, Xiao X, Wen Y, He A, Guo X, Zhang F
Spine41(20)E1244-E1248(2016 Oct)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CILP rs2073711 TT is associated with increased risk of symmetrical hand osteoarthritis particularly in individuals with low variation in work tasks.
Title: Functional polymorphisms in asporin and CILP together with joint loading predispose to hand osteoarthritis.

Meta-analysis. Our results confirm the positive association between CILP and intervertebral disc degeneration, providing novel clues for clarifying the role of CILP in the development of IVD.
Title: Association Between Cartilage Intermediate Layer Protein and Degeneration of Intervertebral Disc: A Meta-analysis.

CILP is involved in the etiology of intervertebral disc degeneration among young adults.
Title: Genetic susceptibility of intervertebral disc degeneration among young Finnish adults.

The researchers found that the single nucleotide polymorphism (1184T/C) of the CILP gene is associated an increased risk of lumbar disc degeneration in male athletes.
Title: Cartilage intermediate layer protein gene is associated with lumbar disc degeneration in male, but not female, collegiate athletes.

the CILP gene 1184T/C polymorphism is a significant risk factor for lumbar disc degeneration occurrence in Japanese collegiate judo athletes
Title: The cartilage intermediate layer protein gene is associated with lumbar disc degeneration in collegiate judokas.

The following CILP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CILP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu107311	NM_003613.4 Latest version!	Homo sapiens cartilage intermediate layer protein (CILP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu85710	XM_017022678.2 Latest version!	Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu85711	XM_017022679.1 Latest version!	Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu19478	NM_003613.3 Latest version!	Homo sapiens cartilage intermediate layer protein (CILP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu85710	XM_017022678.1	Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CILP ( NM_003613.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003604.4 Homo sapiens cartilage intermediate layer protein (CILP), mRNA.

Price & Availability↑

CloneID	OHu107311
Clone ID Related Accession (Same CDS sequence)	NM_003613.4
Accession Version	NM_003613.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	cartilage intermediate layer protein 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068213.7. On Nov 23, 2018 this sequence version replaced NM_003613.3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313352.1, AY358904.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467146, SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261883.6/ ENSP00000261883.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGGGA CCAAGGCCTG GGTGTTCTCC TTCCTGGTCC TGGAAGTCAC ATCTGTGTTG 
GGGAGACAGA CGATGCTCAC CCAGTCAGTA AGAAGAGTCC AGCCTGGGAA GAAGAACCCC 
AGCATCTTTG CCAAGCCTGC CGACACCCTG GAGAGCCCTG GTGAGTGGAC AACATGGTTC 
AACATCGACT ACCCAGGCGG GAAGGGCGAC TATGAGCGGC TGGACGCCAT TCGCTTCTAC 
TATGGGGACC GTGTATGTGC CCGTCCCCTG CGGCTAGAGG CTCGGACCAC TGACTGGACA 
CCTGCGGGCA GCACTGGCCA GGTGGTCCAT GGTAGTCCCC GTGAGGGTTT CTGGTGCCTC 
AACAGGGAGC AGCGGCCTGG CCAGAACTGC TCTAATTACA CCGTACGCTT CCTCTGCCCA 
CCAGGATCCC TGCGCCGAGA CACAGAGCGC ATCTGGAGCC CATGGTCTCC CTGGAGCAAG 
TGCTCAGCTG CCTGTGGTCA GACTGGGGTC CAGACTCGCA CACGCATTTG CTTGGCAGAG 
ATGGTGTCGC TGTGCAGTGA GGCCAGCGAA GAGGGTCAGC ACTGCATGGG CCAGGACTGT 
ACAGCCTGTG ACCTGACCTG CCCAATGGGC CAGGTGAATG CTGACTGTGA TGCCTGCATG 
TGCCAGGACT TCATGCTTCA TGGGGCTGTC TCCCTTCCCG GAGGTGCCCC AGCCTCAGGG 
GCTGCTATCT ACCTCCTGAC CAAGACGCCG AAGCTGCTGA CCCAGACAGA CAGTGATGGG 
AGATTCCGAA TCCCTGGCTT GTGCCCTGAT GGCAAAAGCA TCCTGAAGAT CACAAAGGTC 
AAGTTTGCCC CCATTGTACT CACAATGCCC AAGACTAGCC TGAAGGCAGC CACCATCAAG 
GCAGAGTTTG TGAGGGCAGA GACTCCATAC ATGGTGATGA ACCCTGAGAC AAAAGCACGG 
AGAGCTGGGC AGAGCGTGTC TCTGTGCTGT AAGGCCACAG GGAAGCCCAG GCCAGACAAG 
TATTTTTGGT ATCATAATGA CACATTGCTG GATCCTTCCC TCTACAAGCA TGAGAGCAAG 
CTGGTGCTGA GGAAACTGCA GCAGCACCAG GCTGGGGAGT ACTTTTGCAA GGCCCAGAGT 
GATGCTGGGG CTGTGAAGTC CAAGGTTGCC CAGCTGATTG TCATAGCATC TGATGAGACT 
CCTTGCAACC CAGTTCCTGA GAGCTATCTT ATCCGGCTGC CCCATGATTG CTTTCAGAAT 
GCCACCAACT CCTTCTACTA TGACGTGGGA CGCTGCCCTG TTAAGACTTG TGCAGGGCAG 
CAGGATAATG GGATCAGGTG CCGTGATGCT GTGCAGAACT GCTGTGGCAT CTCCAAGACA 
GAGGAAAGGG AGATCCAGTG CAGTGGCTAC ACGCTACCCA CCAAGGTGGC CAAGGAGTGC 
AGCTGCCAGC GGTGTACGGA AACTCGGAGC ATCGTGCGGG GCCGTGTCAG TGCTGCTGAC 
AATGGGGAGC CCATGCGCTT TGGCCATGTG TACATGGGGA ACAGCCGTGT AAGCATGACT 
GGCTACAAGG GCACTTTCAC CCTCCATGTC CCCCAGGACA CTGAGAGGCT GGTGCTCACA 
TTTGTGGACA GGCTGCAGAA GTTTGTCAAC ACCACCAAAG TGCTACCTTT CAACAAGAAG 
GGGAGTGCCG TGTTCCATGA AATCAAGATG CTTCGTCGGA AAAAGCCCAT CACTTTGGAA 
GCCATGGAGA CCAACATCAT CCCCCTGGGG GAAGTGGTTG GTGAAGACCC CATGGCTGAA 
CTGGAGATTC CATCCAGGAG TTTCTACAGG CAGAATGGGG AGCCCTACAT AGGAAAAGTG 
AAGGCCAGTG TGACCTTCCT GGATCCCCGG AATATTTCCA CAGCCACAGC TGCCCAGACT 
GACCTGAACT TCATCAATGA CGAAGGAGAC ACTTTCCCCC TTCGGACGTA TGGCATGTTC 
TCTGTGGACT TCAGAGATGA GGTCACCTCA GAGCCACTTA ATGCTGGCAA AGTGAAGGTC 
CACCTTGACT CGACCCAGGT CAAGATGCCA GAGCACATAT CCACAGTGAA ACTCTGGTCA 
CTCAATCCAG ACACAGGGCT GTGGGAGGAG GAAGGTGATT TCAAATTTGA AAATCAAAGG 
AGGAACAAAA GAGAAGACAG AACCTTCCTG GTGGGCAACC TGGAGATTCG TGAGAGGAGG 
CTCTTTAACC TGGATGTTCC TGAAAGCAGG CGGTGCTTTG TTAAGGTGAG GGCCTACCGG 
AGTGAGAGGT TCTTGCCTAG TGAGCAGATC CAGGGGGTTG TGATCTCCGT GATTAACCTG 
GAGCCTAGAA CTGGCTTCTT GTCCAACCCT AGGGCCTGGG GCCGCTTTGA CAGTGTCATC 
ACAGGCCCCA ACGGGGCCTG TGTGCCTGCC TTCTGTGATG ACCAGTCCCC TGATGCCTAC 
TCTGCCTATG TCTTGGCAAG CCTGGCTGGG GAGGAACTGC AAGCAGTGGA GTCTTCTCCT 
AAATTCAACC CAAATGCAAT TGGCGTCCCT CAGCCCTATC TCAACAAGCT CAACTACCGT 
CGGACGGACC ATGAGGATCC ACGGGTTAAA AAGACAGCTT TCCAGATTAG CATGGCCAAG 
CCAAGGCCCA ACTCAGCTGA GGAGAGCAAT GGGCCCATCT ATGCCTTTGA GAACCTCCGG 
GCATGTGAAG AGGCACCACC CAGTGCAGCC CACTTCCGGT TCTACCAGAT TGAGGGGGAT 
CGATATGACT ACAACACAGT CCCCTTCAAC GAAGATGACC CTATGAGCTG GACTGAAGAC 
TATCTGGCAT GGTGGCCAAA GCCGATGGAA TTCAGGGCCT GCTATATCAA GGTGAAGATT 
GTGGGGCCAC TGGAAGTGAA TGTGCGATCC CGCAACATGG GGGGCACTCA TCGGCAGACA 
GTGGGGAAGC TGTATGGAAT CCGAGATGTG AGGAGCACTC GGGACAGGGA CCAGCCCAAT 
GTCTCAGCTG CCTGTCTGGA GTTCAAGTGC AGTGGGATGC TCTATGATCA GGACCGTGTG 
GACCGCACCC TGGTGAAGGT CATCCCCCAG GGCAGCTGCC GTCGAGCCAG TGTGAACCCC 
ATGCTGCATG AGTACCTGGT CAACCACTTG CCACTTGCAG TCAACAACGA CACCAGTGAG 
TACACCATGC TGGCACCCTT GGACCCACTG GGCCACAACT ATGGCATCTA CACTGTCACT 
GACCAGGACC CTCGCACGGC CAAGGAGATC GCGCTCGGCC GGTGCTTTGA TGGCACATCC 
GATGGCTCCT CCAGAATCAT GAAGAGCAAT GTGGGAGTAG CCCTCACCTT CAACTGTGTA 
GAGAGGCAAG TAGGCCGCCA GAGTGCCTTC CAGTACCTCC AAAGCACCCC AGCCCAGTCC 
CCTGCTGCAG GCACTGTCCA AGGAAGAGTG CCCTCGAGGA GGCAGCAGCG AGCGAGCAGG 
GGTGGCCAGC GCCAGGGTGG AGTGGTGGCC TCTCTGAGAT TTCCTAGAGT TGCTCAACAG 
CCCCTGATCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003604.4
CDS	153..3707
Translation	MVGTKAWVFSFLVLEVTSVLGRQTMLTQSVRRVQPGKKNPSIFAKPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSKCSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDACMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILKITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATGKPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFGHVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRRKKPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASVTFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHLDSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN

Target ORF information:

RefSeq Version	NM_003613.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cartilage intermediate layer protein (CILP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003613.4

ORF Insert Sequence:

ATGGTGGGGA CCAAGGCCTG GGTGTTCTCC TTCCTGGTCC TGGAAGTCAC ATCTGTGTTG 
GGGAGACAGA CGATGCTCAC CCAGTCAGTA AGAAGAGTCC AGCCTGGGAA GAAGAACCCC 
AGCATCTTTG CCAAGCCTGC CGACACCCTG GAGAGCCCTG GTGAGTGGAC AACATGGTTC 
AACATCGACT ACCCAGGCGG GAAGGGCGAC TATGAGCGGC TGGACGCCAT TCGCTTCTAC 
TATGGGGACC GTGTATGTGC CCGTCCCCTG CGGCTAGAGG CTCGGACCAC TGACTGGACA 
CCTGCGGGCA GCACTGGCCA GGTGGTCCAT GGTAGTCCCC GTGAGGGTTT CTGGTGCCTC 
AACAGGGAGC AGCGGCCTGG CCAGAACTGC TCTAATTACA CCGTACGCTT CCTCTGCCCA 
CCAGGATCCC TGCGCCGAGA CACAGAGCGC ATCTGGAGCC CATGGTCTCC CTGGAGCAAG 
TGCTCAGCTG CCTGTGGTCA GACTGGGGTC CAGACTCGCA CACGCATTTG CTTGGCAGAG 
ATGGTGTCGC TGTGCAGTGA GGCCAGCGAA GAGGGTCAGC ACTGCATGGG CCAGGACTGT 
ACAGCCTGTG ACCTGACCTG CCCAATGGGC CAGGTGAATG CTGACTGTGA TGCCTGCATG 
TGCCAGGACT TCATGCTTCA TGGGGCTGTC TCCCTTCCCG GAGGTGCCCC AGCCTCAGGG 
GCTGCTATCT ACCTCCTGAC CAAGACGCCG AAGCTGCTGA CCCAGACAGA CAGTGATGGG 
AGATTCCGAA TCCCTGGCTT GTGCCCTGAT GGCAAAAGCA TCCTGAAGAT CACAAAGGTC 
AAGTTTGCCC CCATTGTACT CACAATGCCC AAGACTAGCC TGAAGGCAGC CACCATCAAG 
GCAGAGTTTG TGAGGGCAGA GACTCCATAC ATGGTGATGA ACCCTGAGAC AAAAGCACGG 
AGAGCTGGGC AGAGCGTGTC TCTGTGCTGT AAGGCCACAG GGAAGCCCAG GCCAGACAAG 
TATTTTTGGT ATCATAATGA CACATTGCTG GATCCTTCCC TCTACAAGCA TGAGAGCAAG 
CTGGTGCTGA GGAAACTGCA GCAGCACCAG GCTGGGGAGT ACTTTTGCAA GGCCCAGAGT 
GATGCTGGGG CTGTGAAGTC CAAGGTTGCC CAGCTGATTG TCATAGCATC TGATGAGACT 
CCTTGCAACC CAGTTCCTGA GAGCTATCTT ATCCGGCTGC CCCATGATTG CTTTCAGAAT 
GCCACCAACT CCTTCTACTA TGACGTGGGA CGCTGCCCTG TTAAGACTTG TGCAGGGCAG 
CAGGATAATG GGATCAGGTG CCGTGATGCT GTGCAGAACT GCTGTGGCAT CTCCAAGACA 
GAGGAAAGGG AGATCCAGTG CAGTGGCTAC ACGCTACCCA CCAAGGTGGC CAAGGAGTGC 
AGCTGCCAGC GGTGTACGGA AACTCGGAGC ATCGTGCGGG GCCGTGTCAG TGCTGCTGAC 
AATGGGGAGC CCATGCGCTT TGGCCATGTG TACATGGGGA ACAGCCGTGT AAGCATGACT 
GGCTACAAGG GCACTTTCAC CCTCCATGTC CCCCAGGACA CTGAGAGGCT GGTGCTCACA 
TTTGTGGACA GGCTGCAGAA GTTTGTCAAC ACCACCAAAG TGCTACCTTT CAACAAGAAG 
GGGAGTGCCG TGTTCCATGA AATCAAGATG CTTCGTCGGA AAAAGCCCAT CACTTTGGAA 
GCCATGGAGA CCAACATCAT CCCCCTGGGG GAAGTGGTTG GTGAAGACCC CATGGCTGAA 
CTGGAGATTC CATCCAGGAG TTTCTACAGG CAGAATGGGG AGCCCTACAT AGGAAAAGTG 
AAGGCCAGTG TGACCTTCCT GGATCCCCGG AATATTTCCA CAGCCACAGC TGCCCAGACT 
GACCTGAACT TCATCAATGA CGAAGGAGAC ACTTTCCCCC TTCGGACGTA TGGCATGTTC 
TCTGTGGACT TCAGAGATGA GGTCACCTCA GAGCCACTTA ATGCTGGCAA AGTGAAGGTC 
CACCTTGACT CGACCCAGGT CAAGATGCCA GAGCACATAT CCACAGTGAA ACTCTGGTCA 
CTCAATCCAG ACACAGGGCT GTGGGAGGAG GAAGGTGATT TCAAATTTGA AAATCAAAGG 
AGGAACAAAA GAGAAGACAG AACCTTCCTG GTGGGCAACC TGGAGATTCG TGAGAGGAGG 
CTCTTTAACC TGGATGTTCC TGAAAGCAGG CGGTGCTTTG TTAAGGTGAG GGCCTACCGG 
AGTGAGAGGT TCTTGCCTAG TGAGCAGATC CAGGGGGTTG TGATCTCCGT GATTAACCTG 
GAGCCTAGAA CTGGCTTCTT GTCCAACCCT AGGGCCTGGG GCCGCTTTGA CAGTGTCATC 
ACAGGCCCCA ACGGGGCCTG TGTGCCTGCC TTCTGTGATG ACCAGTCCCC TGATGCCTAC 
TCTGCCTATG TCTTGGCAAG CCTGGCTGGG GAGGAACTGC AAGCAGTGGA GTCTTCTCCT 
AAATTCAACC CAAATGCAAT TGGCGTCCCT CAGCCCTATC TCAACAAGCT CAACTACCGT 
CGGACGGACC ATGAGGATCC ACGGGTTAAA AAGACAGCTT TCCAGATTAG CATGGCCAAG 
CCAAGGCCCA ACTCAGCTGA GGAGAGCAAT GGGCCCATCT ATGCCTTTGA GAACCTCCGG 
GCATGTGAAG AGGCACCACC CAGTGCAGCC CACTTCCGGT TCTACCAGAT TGAGGGGGAT 
CGATATGACT ACAACACAGT CCCCTTCAAC GAAGATGACC CTATGAGCTG GACTGAAGAC 
TATCTGGCAT GGTGGCCAAA GCCGATGGAA TTCAGGGCCT GCTATATCAA GGTGAAGATT 
GTGGGGCCAC TGGAAGTGAA TGTGCGATCC CGCAACATGG GGGGCACTCA TCGGCAGACA 
GTGGGGAAGC TGTATGGAAT CCGAGATGTG AGGAGCACTC GGGACAGGGA CCAGCCCAAT 
GTCTCAGCTG CCTGTCTGGA GTTCAAGTGC AGTGGGATGC TCTATGATCA GGACCGTGTG 
GACCGCACCC TGGTGAAGGT CATCCCCCAG GGCAGCTGCC GTCGAGCCAG TGTGAACCCC 
ATGCTGCATG AGTACCTGGT CAACCACTTG CCACTTGCAG TCAACAACGA CACCAGTGAG 
TACACCATGC TGGCACCCTT GGACCCACTG GGCCACAACT ATGGCATCTA CACTGTCACT 
GACCAGGACC CTCGCACGGC CAAGGAGATC GCGCTCGGCC GGTGCTTTGA TGGCACATCC 
GATGGCTCCT CCAGAATCAT GAAGAGCAAT GTGGGAGTAG CCCTCACCTT CAACTGTGTA 
GAGAGGCAAG TAGGCCGCCA GAGTGCCTTC CAGTACCTCC AAAGCACCCC AGCCCAGTCC 
CCTGCTGCAG GCACTGTCCA AGGAAGAGTG CCCTCGAGGA GGCAGCAGCG AGCGAGCAGG 
GGTGGCCAGC GCCAGGGTGG AGTGGTGGCC TCTCTGAGAT TTCCTAGAGT TGCTCAACAG 
CCCCTGATCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_017022678.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878167.1 Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85710
Clone ID Related Accession (Same CDS sequence)	XM_017022678.2 , XM_017022678.1
Accession Version	XM_017022678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cartilage intermediate layer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAT CTGGTTTCGC TGCTGCTTCC TCTGGCTGGC TGCCACAGGC TGAGCCACCA 
GCTGTGCTAT ATAAGGCCGG CACTGGACTG CTGGAGAGGC GAAGGCTTGG ACCCCCAGCT 
GAGGAGTCCT GCTCAAGACA CGGGAGACAG ACGATGCTCA CCCAGTCAGT AAGAAGAGTC 
CAGCCTGGGA AGAAGAACCC CAGCATCTTT GCCAAGCCTG CCGACACCCT GGAGAGCCCT 
GGTGAGTGGA CAACATGGTT CAACATCGAC TACCCAGGCG GGAAGGGCGA CTATGAGCGG 
CTGGACGCCA TTCGCTTCTA CTATGGGGAC CGTGTATGTG CCCGTCCCCT GCGGCTAGAG 
GCTCGGACCA CTGACTGGAC ACCTGCGGGC AGCACTGGCC AGGTGGTCCA TGGTAGTCCC 
CGTGAGGGTT TCTGGTGCCT CAACAGGGAG CAGCGGCCTG GCCAGAACTG CTCTAATTAC 
ACCGTACGCT TCCTCTGCCC ACCAGGATCC CTGCGCCGAG ACACAGAGCG CATCTGGAGC 
CCATGGTCTC CCTGGAGCAA GTGCTCAGCT GCCTGTGGTC AGACTGGGGT CCAGACTCGC 
ACACGCATTT GCTTGGCAGA GATGGTGTCG CTGTGCAGTG AGGCCAGCGA AGAGGGTCAG 
CACTGCATGG GCCAGGACTG TACAGCCTGT GACCTGACCT GCCCAATGGG CCAGGTGAAT 
GCTGACTGTG ATGCCTGCAT GTGCCAGGAC TTCATGCTTC ATGGGGCTGT CTCCCTTCCC 
GGAGGTGCCC CAGCCTCAGG GGCTGCTATC TACCTCCTGA CCAAGACGCC GAAGCTGCTG 
ACCCAGACAG ACAGTGATGG GAGATTCCGA ATCCCTGGCT TGTGCCCTGA TGGCAAAAGC 
ATCCTGAAGA TCACAAAGGT CAAGTTTGCC CCCATTGTAC TCACAATGCC CAAGACTAGC 
CTGAAGGCAG CCACCATCAA GGCAGAGTTT GTGAGGGCAG AGACTCCATA CATGGTGATG 
AACCCTGAGA CAAAAGCACG GAGAGCTGGG CAGAGCGTGT CTCTGTGCTG TAAGGCCACA 
GGGAAGCCCA GGCCAGACAA GTATTTTTGG TATCATAATG ACACATTGCT GGATCCTTCC 
CTCTACAAGC ATGAGAGCAA GCTGGTGCTG AGGAAACTGC AGCAGCACCA GGCTGGGGAG 
TACTTTTGCA AGGCCCAGAG TGATGCTGGG GCTGTGAAGT CCAAGGTTGC CCAGCTGATT 
GTCATAGCAT CTGATGAGAC TCCTTGCAAC CCAGTTCCTG AGAGCTATCT TATCCGGCTG 
CCCCATGATT GCTTTCAGAA TGCCACCAAC TCCTTCTACT ATGACGTGGG ACGCTGCCCT 
GTTAAGACTT GTGCAGGGCA GCAGGATAAT GGGATCAGGT GCCGTGATGC TGTGCAGAAC 
TGCTGTGGCA TCTCCAAGAC AGAGGAAAGG GAGATCCAGT GCAGTGGCTA CACGCTACCC 
ACCAAGGTGG CCAAGGAGTG CAGCTGCCAG CGGTGTACGG AAACTCGGAG CATCGTGCGG 
GGCCGTGTCA GTGCTGCTGA CAATGGGGAG CCCATGCGCT TTGGCCATGT GTACATGGGG 
AACAGCCGTG TAAGCATGAC TGGCTACAAG GGCACTTTCA CCCTCCATGT CCCCCAGGAC 
ACTGAGAGGC TGGTGCTCAC ATTTGTGGAC AGGCTGCAGA AGTTTGTCAA CACCACCAAA 
GTGCTACCTT TCAACAAGAA GGGGAGTGCC GTGTTCCATG AAATCAAGAT GCTTCGTCGG 
AAAAAGCCCA TCACTTTGGA AGCCATGGAG ACCAACATCA TCCCCCTGGG GGAAGTGGTT 
GGTGAAGACC CCATGGCTGA ACTGGAGATT CCATCCAGGA GTTTCTACAG GCAGAATGGG 
GAGCCCTACA TAGGAAAAGT GAAGGCCAGT GTGACCTTCC TGGATCCCCG GAATATTTCC 
ACAGCCACAG CTGCCCAGAC TGACCTGAAC TTCATCAATG ACGAAGGAGA CACTTTCCCC 
CTTCGGACGT ATGGCATGTT CTCTGTGGAC TTCAGAGATG AGGTCACCTC AGAGCCACTT 
AATGCTGGCA AAGTGAAGGT CCACCTTGAC TCGACCCAGG TCAAGATGCC AGAGCACATA 
TCCACAGTGA AACTCTGGTC ACTCAATCCA GACACAGGGC TGTGGGAGGA GGAAGGTGAT 
TTCAAATTTG AAAATCAAAG GAGGAACAAA AGAGAAGACA GAACCTTCCT GGTGGGCAAC 
CTGGAGATTC GTGAGAGGAG GCTCTTTAAC CTGGATGTTC CTGAAAGCAG GCGGTGCTTT 
GTTAAGGTGA GGGCCTACCG GAGTGAGAGG TTCTTGCCTA GTGAGCAGAT CCAGGGGGTT 
GTGATCTCCG TGATTAACCT GGAGCCTAGA ACTGGCTTCT TGTCCAACCC TAGGGCCTGG 
GGCCGCTTTG ACAGTGTCAT CACAGGCCCC AACGGGGCCT GTGTGCCTGC CTTCTGTGAT 
GACCAGTCCC CTGATGCCTA CTCTGCCTAT GTCTTGGCAA GCCTGGCTGG GGAGGAACTG 
CAAGCAGTGG AGTCTTCTCC TAAATTCAAC CCAAATGCAA TTGGCGTCCC TCAGCCCTAT 
CTCAACAAGC TCAACTACCG TCGGACGGAC CATGAGGATC CACGGGTTAA AAAGACAGCT 
TTCCAGATTA GCATGGCCAA GCCAAGGCCC AACTCAGCTG AGGAGAGCAA TGGGCCCATC 
TATGCCTTTG AGAACCTCCG GGCATGTGAA GAGGCACCAC CCAGTGCAGC CCACTTCCGG 
TTCTACCAGA TTGAGGGGGA TCGATATGAC TACAACACAG TCCCCTTCAA CGAAGATGAC 
CCTATGAGCT GGACTGAAGA CTATCTGGCA TGGTGGCCAA AGCCGATGGA ATTCAGGGCC 
TGCTATATCA AGGTGAAGAT TGTGGGGCCA CTGGAAGTGA ATGTGCGATC CCGCAACATG 
GGGGGCACTC ATCGGCAGAC AGTGGGGAAG CTGTATGGAA TCCGAGATGT GAGGAGCACT 
CGGGACAGGG ACCAGCCCAA TGTCTCAGCT GCCTGTCTGG AGTTCAAGTG CAGTGGGATG 
CTCTATGATC AGGACCGTGT GGACCGCACC CTGGTGAAGG TCATCCCCCA GGGCAGCTGC 
CGTCGAGCCA GTGTGAACCC CATGCTGCAT GAGTACCTGG TCAACCACTT GCCACTTGCA 
GTCAACAACG ACACCAGTGA GTACACCATG CTGGCACCCT TGGACCCACT GGGCCACAAC 
TATGGCATCT ACACTGTCAC TGACCAGGAC CCTCGCACGG CCAAGGAGAT CGCGCTCGGC 
CGGTGCTTTG ATGGCACATC CGATGGCTCC TCCAGAATCA TGAAGAGCAA TGTGGGAGTA 
GCCCTCACCT TCAACTGTGT AGAGAGGCAA GTAGGCCGCC AGAGTGCCTT CCAGTACCTC 
CAAAGCACCC CAGCCCAGTC CCCTGCTGCA GGCACTGTCC AAGGAAGAGT GCCCTCGAGG 
AGGCAGCAGC GAGCGAGCAG GGGTGGCCAG CGCCAGGGTG GAGTGGTGGC CTCTCTGAGA 
TTTCCTAGAG TTGCTCAACA GCCCCTGATC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878167.1
CDS	1574..5209
Translation	MLRSGFAAASSGWLPQAEPPAVLYKAGTGLLERRRLGPPAEESCSRHGRQTMLTQSVRRVQPGKKNPSIFAKPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSKCSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDACMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILKITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATGKPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFGHVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRRKKPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASVTFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHLDSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN

Target ORF information:

RefSeq Version	XM_017022678.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022678.2

ORF Insert Sequence:

ATGCTCAGAT CTGGTTTCGC TGCTGCTTCC TCTGGCTGGC TGCCACAGGC TGAGCCACCA 
GCTGTGCTAT ATAAGGCCGG CACTGGACTG CTGGAGAGGC GAAGGCTTGG ACCCCCAGCT 
GAGGAGTCCT GCTCAAGACA CGGGAGACAG ACGATGCTCA CCCAGTCAGT AAGAAGAGTC 
CAGCCTGGGA AGAAGAACCC CAGCATCTTT GCCAAGCCTG CCGACACCCT GGAGAGCCCT 
GGTGAGTGGA CAACATGGTT CAACATCGAC TACCCAGGCG GGAAGGGCGA CTATGAGCGG 
CTGGACGCCA TTCGCTTCTA CTATGGGGAC CGTGTATGTG CCCGTCCCCT GCGGCTAGAG 
GCTCGGACCA CTGACTGGAC ACCTGCGGGC AGCACTGGCC AGGTGGTCCA TGGTAGTCCC 
CGTGAGGGTT TCTGGTGCCT CAACAGGGAG CAGCGGCCTG GCCAGAACTG CTCTAATTAC 
ACCGTACGCT TCCTCTGCCC ACCAGGATCC CTGCGCCGAG ACACAGAGCG CATCTGGAGC 
CCATGGTCTC CCTGGAGCAA GTGCTCAGCT GCCTGTGGTC AGACTGGGGT CCAGACTCGC 
ACACGCATTT GCTTGGCAGA GATGGTGTCG CTGTGCAGTG AGGCCAGCGA AGAGGGTCAG 
CACTGCATGG GCCAGGACTG TACAGCCTGT GACCTGACCT GCCCAATGGG CCAGGTGAAT 
GCTGACTGTG ATGCCTGCAT GTGCCAGGAC TTCATGCTTC ATGGGGCTGT CTCCCTTCCC 
GGAGGTGCCC CAGCCTCAGG GGCTGCTATC TACCTCCTGA CCAAGACGCC GAAGCTGCTG 
ACCCAGACAG ACAGTGATGG GAGATTCCGA ATCCCTGGCT TGTGCCCTGA TGGCAAAAGC 
ATCCTGAAGA TCACAAAGGT CAAGTTTGCC CCCATTGTAC TCACAATGCC CAAGACTAGC 
CTGAAGGCAG CCACCATCAA GGCAGAGTTT GTGAGGGCAG AGACTCCATA CATGGTGATG 
AACCCTGAGA CAAAAGCACG GAGAGCTGGG CAGAGCGTGT CTCTGTGCTG TAAGGCCACA 
GGGAAGCCCA GGCCAGACAA GTATTTTTGG TATCATAATG ACACATTGCT GGATCCTTCC 
CTCTACAAGC ATGAGAGCAA GCTGGTGCTG AGGAAACTGC AGCAGCACCA GGCTGGGGAG 
TACTTTTGCA AGGCCCAGAG TGATGCTGGG GCTGTGAAGT CCAAGGTTGC CCAGCTGATT 
GTCATAGCAT CTGATGAGAC TCCTTGCAAC CCAGTTCCTG AGAGCTATCT TATCCGGCTG 
CCCCATGATT GCTTTCAGAA TGCCACCAAC TCCTTCTACT ATGACGTGGG ACGCTGCCCT 
GTTAAGACTT GTGCAGGGCA GCAGGATAAT GGGATCAGGT GCCGTGATGC TGTGCAGAAC 
TGCTGTGGCA TCTCCAAGAC AGAGGAAAGG GAGATCCAGT GCAGTGGCTA CACGCTACCC 
ACCAAGGTGG CCAAGGAGTG CAGCTGCCAG CGGTGTACGG AAACTCGGAG CATCGTGCGG 
GGCCGTGTCA GTGCTGCTGA CAATGGGGAG CCCATGCGCT TTGGCCATGT GTACATGGGG 
AACAGCCGTG TAAGCATGAC TGGCTACAAG GGCACTTTCA CCCTCCATGT CCCCCAGGAC 
ACTGAGAGGC TGGTGCTCAC ATTTGTGGAC AGGCTGCAGA AGTTTGTCAA CACCACCAAA 
GTGCTACCTT TCAACAAGAA GGGGAGTGCC GTGTTCCATG AAATCAAGAT GCTTCGTCGG 
AAAAAGCCCA TCACTTTGGA AGCCATGGAG ACCAACATCA TCCCCCTGGG GGAAGTGGTT 
GGTGAAGACC CCATGGCTGA ACTGGAGATT CCATCCAGGA GTTTCTACAG GCAGAATGGG 
GAGCCCTACA TAGGAAAAGT GAAGGCCAGT GTGACCTTCC TGGATCCCCG GAATATTTCC 
ACAGCCACAG CTGCCCAGAC TGACCTGAAC TTCATCAATG ACGAAGGAGA CACTTTCCCC 
CTTCGGACGT ATGGCATGTT CTCTGTGGAC TTCAGAGATG AGGTCACCTC AGAGCCACTT 
AATGCTGGCA AAGTGAAGGT CCACCTTGAC TCGACCCAGG TCAAGATGCC AGAGCACATA 
TCCACAGTGA AACTCTGGTC ACTCAATCCA GACACAGGGC TGTGGGAGGA GGAAGGTGAT 
TTCAAATTTG AAAATCAAAG GAGGAACAAA AGAGAAGACA GAACCTTCCT GGTGGGCAAC 
CTGGAGATTC GTGAGAGGAG GCTCTTTAAC CTGGATGTTC CTGAAAGCAG GCGGTGCTTT 
GTTAAGGTGA GGGCCTACCG GAGTGAGAGG TTCTTGCCTA GTGAGCAGAT CCAGGGGGTT 
GTGATCTCCG TGATTAACCT GGAGCCTAGA ACTGGCTTCT TGTCCAACCC TAGGGCCTGG 
GGCCGCTTTG ACAGTGTCAT CACAGGCCCC AACGGGGCCT GTGTGCCTGC CTTCTGTGAT 
GACCAGTCCC CTGATGCCTA CTCTGCCTAT GTCTTGGCAA GCCTGGCTGG GGAGGAACTG 
CAAGCAGTGG AGTCTTCTCC TAAATTCAAC CCAAATGCAA TTGGCGTCCC TCAGCCCTAT 
CTCAACAAGC TCAACTACCG TCGGACGGAC CATGAGGATC CACGGGTTAA AAAGACAGCT 
TTCCAGATTA GCATGGCCAA GCCAAGGCCC AACTCAGCTG AGGAGAGCAA TGGGCCCATC 
TATGCCTTTG AGAACCTCCG GGCATGTGAA GAGGCACCAC CCAGTGCAGC CCACTTCCGG 
TTCTACCAGA TTGAGGGGGA TCGATATGAC TACAACACAG TCCCCTTCAA CGAAGATGAC 
CCTATGAGCT GGACTGAAGA CTATCTGGCA TGGTGGCCAA AGCCGATGGA ATTCAGGGCC 
TGCTATATCA AGGTGAAGAT TGTGGGGCCA CTGGAAGTGA ATGTGCGATC CCGCAACATG 
GGGGGCACTC ATCGGCAGAC AGTGGGGAAG CTGTATGGAA TCCGAGATGT GAGGAGCACT 
CGGGACAGGG ACCAGCCCAA TGTCTCAGCT GCCTGTCTGG AGTTCAAGTG CAGTGGGATG 
CTCTATGATC AGGACCGTGT GGACCGCACC CTGGTGAAGG TCATCCCCCA GGGCAGCTGC 
CGTCGAGCCA GTGTGAACCC CATGCTGCAT GAGTACCTGG TCAACCACTT GCCACTTGCA 
GTCAACAACG ACACCAGTGA GTACACCATG CTGGCACCCT TGGACCCACT GGGCCACAAC 
TATGGCATCT ACACTGTCAC TGACCAGGAC CCTCGCACGG CCAAGGAGAT CGCGCTCGGC 
CGGTGCTTTG ATGGCACATC CGATGGCTCC TCCAGAATCA TGAAGAGCAA TGTGGGAGTA 
GCCCTCACCT TCAACTGTGT AGAGAGGCAA GTAGGCCGCC AGAGTGCCTT CCAGTACCTC 
CAAAGCACCC CAGCCCAGTC CCCTGCTGCA GGCACTGTCC AAGGAAGAGT GCCCTCGAGG 
AGGCAGCAGC GAGCGAGCAG GGGTGGCCAG CGCCAGGGTG GAGTGGTGGC CTCTCTGAGA 
TTTCCTAGAG TTGCTCAACA GCCCCTGATC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_017022678.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878167.1 Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

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CloneID	OHu85710
Clone ID Related Accession (Same CDS sequence)	XM_017022678.2 , XM_017022678.1
Accession Version	XM_017022678.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cartilage intermediate layer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAT CTGGTTTCGC TGCTGCTTCC TCTGGCTGGC TGCCACAGGC TGAGCCACCA 
GCTGTGCTAT ATAAGGCCGG CACTGGACTG CTGGAGAGGC GAAGGCTTGG ACCCCCAGCT 
GAGGAGTCCT GCTCAAGACA CGGGAGACAG ACGATGCTCA CCCAGTCAGT AAGAAGAGTC 
CAGCCTGGGA AGAAGAACCC CAGCATCTTT GCCAAGCCTG CCGACACCCT GGAGAGCCCT 
GGTGAGTGGA CAACATGGTT CAACATCGAC TACCCAGGCG GGAAGGGCGA CTATGAGCGG 
CTGGACGCCA TTCGCTTCTA CTATGGGGAC CGTGTATGTG CCCGTCCCCT GCGGCTAGAG 
GCTCGGACCA CTGACTGGAC ACCTGCGGGC AGCACTGGCC AGGTGGTCCA TGGTAGTCCC 
CGTGAGGGTT TCTGGTGCCT CAACAGGGAG CAGCGGCCTG GCCAGAACTG CTCTAATTAC 
ACCGTACGCT TCCTCTGCCC ACCAGGATCC CTGCGCCGAG ACACAGAGCG CATCTGGAGC 
CCATGGTCTC CCTGGAGCAA GTGCTCAGCT GCCTGTGGTC AGACTGGGGT CCAGACTCGC 
ACACGCATTT GCTTGGCAGA GATGGTGTCG CTGTGCAGTG AGGCCAGCGA AGAGGGTCAG 
CACTGCATGG GCCAGGACTG TACAGCCTGT GACCTGACCT GCCCAATGGG CCAGGTGAAT 
GCTGACTGTG ATGCCTGCAT GTGCCAGGAC TTCATGCTTC ATGGGGCTGT CTCCCTTCCC 
GGAGGTGCCC CAGCCTCAGG GGCTGCTATC TACCTCCTGA CCAAGACGCC GAAGCTGCTG 
ACCCAGACAG ACAGTGATGG GAGATTCCGA ATCCCTGGCT TGTGCCCTGA TGGCAAAAGC 
ATCCTGAAGA TCACAAAGGT CAAGTTTGCC CCCATTGTAC TCACAATGCC CAAGACTAGC 
CTGAAGGCAG CCACCATCAA GGCAGAGTTT GTGAGGGCAG AGACTCCATA CATGGTGATG 
AACCCTGAGA CAAAAGCACG GAGAGCTGGG CAGAGCGTGT CTCTGTGCTG TAAGGCCACA 
GGGAAGCCCA GGCCAGACAA GTATTTTTGG TATCATAATG ACACATTGCT GGATCCTTCC 
CTCTACAAGC ATGAGAGCAA GCTGGTGCTG AGGAAACTGC AGCAGCACCA GGCTGGGGAG 
TACTTTTGCA AGGCCCAGAG TGATGCTGGG GCTGTGAAGT CCAAGGTTGC CCAGCTGATT 
GTCATAGCAT CTGATGAGAC TCCTTGCAAC CCAGTTCCTG AGAGCTATCT TATCCGGCTG 
CCCCATGATT GCTTTCAGAA TGCCACCAAC TCCTTCTACT ATGACGTGGG ACGCTGCCCT 
GTTAAGACTT GTGCAGGGCA GCAGGATAAT GGGATCAGGT GCCGTGATGC TGTGCAGAAC 
TGCTGTGGCA TCTCCAAGAC AGAGGAAAGG GAGATCCAGT GCAGTGGCTA CACGCTACCC 
ACCAAGGTGG CCAAGGAGTG CAGCTGCCAG CGGTGTACGG AAACTCGGAG CATCGTGCGG 
GGCCGTGTCA GTGCTGCTGA CAATGGGGAG CCCATGCGCT TTGGCCATGT GTACATGGGG 
AACAGCCGTG TAAGCATGAC TGGCTACAAG GGCACTTTCA CCCTCCATGT CCCCCAGGAC 
ACTGAGAGGC TGGTGCTCAC ATTTGTGGAC AGGCTGCAGA AGTTTGTCAA CACCACCAAA 
GTGCTACCTT TCAACAAGAA GGGGAGTGCC GTGTTCCATG AAATCAAGAT GCTTCGTCGG 
AAAAAGCCCA TCACTTTGGA AGCCATGGAG ACCAACATCA TCCCCCTGGG GGAAGTGGTT 
GGTGAAGACC CCATGGCTGA ACTGGAGATT CCATCCAGGA GTTTCTACAG GCAGAATGGG 
GAGCCCTACA TAGGAAAAGT GAAGGCCAGT GTGACCTTCC TGGATCCCCG GAATATTTCC 
ACAGCCACAG CTGCCCAGAC TGACCTGAAC TTCATCAATG ACGAAGGAGA CACTTTCCCC 
CTTCGGACGT ATGGCATGTT CTCTGTGGAC TTCAGAGATG AGGTCACCTC AGAGCCACTT 
AATGCTGGCA AAGTGAAGGT CCACCTTGAC TCGACCCAGG TCAAGATGCC AGAGCACATA 
TCCACAGTGA AACTCTGGTC ACTCAATCCA GACACAGGGC TGTGGGAGGA GGAAGGTGAT 
TTCAAATTTG AAAATCAAAG GAGGAACAAA AGAGAAGACA GAACCTTCCT GGTGGGCAAC 
CTGGAGATTC GTGAGAGGAG GCTCTTTAAC CTGGATGTTC CTGAAAGCAG GCGGTGCTTT 
GTTAAGGTGA GGGCCTACCG GAGTGAGAGG TTCTTGCCTA GTGAGCAGAT CCAGGGGGTT 
GTGATCTCCG TGATTAACCT GGAGCCTAGA ACTGGCTTCT TGTCCAACCC TAGGGCCTGG 
GGCCGCTTTG ACAGTGTCAT CACAGGCCCC AACGGGGCCT GTGTGCCTGC CTTCTGTGAT 
GACCAGTCCC CTGATGCCTA CTCTGCCTAT GTCTTGGCAA GCCTGGCTGG GGAGGAACTG 
CAAGCAGTGG AGTCTTCTCC TAAATTCAAC CCAAATGCAA TTGGCGTCCC TCAGCCCTAT 
CTCAACAAGC TCAACTACCG TCGGACGGAC CATGAGGATC CACGGGTTAA AAAGACAGCT 
TTCCAGATTA GCATGGCCAA GCCAAGGCCC AACTCAGCTG AGGAGAGCAA TGGGCCCATC 
TATGCCTTTG AGAACCTCCG GGCATGTGAA GAGGCACCAC CCAGTGCAGC CCACTTCCGG 
TTCTACCAGA TTGAGGGGGA TCGATATGAC TACAACACAG TCCCCTTCAA CGAAGATGAC 
CCTATGAGCT GGACTGAAGA CTATCTGGCA TGGTGGCCAA AGCCGATGGA ATTCAGGGCC 
TGCTATATCA AGGTGAAGAT TGTGGGGCCA CTGGAAGTGA ATGTGCGATC CCGCAACATG 
GGGGGCACTC ATCGGCAGAC AGTGGGGAAG CTGTATGGAA TCCGAGATGT GAGGAGCACT 
CGGGACAGGG ACCAGCCCAA TGTCTCAGCT GCCTGTCTGG AGTTCAAGTG CAGTGGGATG 
CTCTATGATC AGGACCGTGT GGACCGCACC CTGGTGAAGG TCATCCCCCA GGGCAGCTGC 
CGTCGAGCCA GTGTGAACCC CATGCTGCAT GAGTACCTGG TCAACCACTT GCCACTTGCA 
GTCAACAACG ACACCAGTGA GTACACCATG CTGGCACCCT TGGACCCACT GGGCCACAAC 
TATGGCATCT ACACTGTCAC TGACCAGGAC CCTCGCACGG CCAAGGAGAT CGCGCTCGGC 
CGGTGCTTTG ATGGCACATC CGATGGCTCC TCCAGAATCA TGAAGAGCAA TGTGGGAGTA 
GCCCTCACCT TCAACTGTGT AGAGAGGCAA GTAGGCCGCC AGAGTGCCTT CCAGTACCTC 
CAAAGCACCC CAGCCCAGTC CCCTGCTGCA GGCACTGTCC AAGGAAGAGT GCCCTCGAGG 
AGGCAGCAGC GAGCGAGCAG GGGTGGCCAG CGCCAGGGTG GAGTGGTGGC CTCTCTGAGA 
TTTCCTAGAG TTGCTCAACA GCCCCTGATC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878167.1
CDS	40..3675
Translation	MLRSGFAAASSGWLPQAEPPAVLYKAGTGLLERRRLGPPAEESCSRHGRQTMLTQSVRRVQPGKKNPSIFAKPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSKCSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDACMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILKITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATGKPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFGHVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRRKKPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASVTFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHLDSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN

Target ORF information:

RefSeq Version	XM_017022678.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022678.1

ORF Insert Sequence:

ATGCTCAGAT CTGGTTTCGC TGCTGCTTCC TCTGGCTGGC TGCCACAGGC TGAGCCACCA 
GCTGTGCTAT ATAAGGCCGG CACTGGACTG CTGGAGAGGC GAAGGCTTGG ACCCCCAGCT 
GAGGAGTCCT GCTCAAGACA CGGGAGACAG ACGATGCTCA CCCAGTCAGT AAGAAGAGTC 
CAGCCTGGGA AGAAGAACCC CAGCATCTTT GCCAAGCCTG CCGACACCCT GGAGAGCCCT 
GGTGAGTGGA CAACATGGTT CAACATCGAC TACCCAGGCG GGAAGGGCGA CTATGAGCGG 
CTGGACGCCA TTCGCTTCTA CTATGGGGAC CGTGTATGTG CCCGTCCCCT GCGGCTAGAG 
GCTCGGACCA CTGACTGGAC ACCTGCGGGC AGCACTGGCC AGGTGGTCCA TGGTAGTCCC 
CGTGAGGGTT TCTGGTGCCT CAACAGGGAG CAGCGGCCTG GCCAGAACTG CTCTAATTAC 
ACCGTACGCT TCCTCTGCCC ACCAGGATCC CTGCGCCGAG ACACAGAGCG CATCTGGAGC 
CCATGGTCTC CCTGGAGCAA GTGCTCAGCT GCCTGTGGTC AGACTGGGGT CCAGACTCGC 
ACACGCATTT GCTTGGCAGA GATGGTGTCG CTGTGCAGTG AGGCCAGCGA AGAGGGTCAG 
CACTGCATGG GCCAGGACTG TACAGCCTGT GACCTGACCT GCCCAATGGG CCAGGTGAAT 
GCTGACTGTG ATGCCTGCAT GTGCCAGGAC TTCATGCTTC ATGGGGCTGT CTCCCTTCCC 
GGAGGTGCCC CAGCCTCAGG GGCTGCTATC TACCTCCTGA CCAAGACGCC GAAGCTGCTG 
ACCCAGACAG ACAGTGATGG GAGATTCCGA ATCCCTGGCT TGTGCCCTGA TGGCAAAAGC 
ATCCTGAAGA TCACAAAGGT CAAGTTTGCC CCCATTGTAC TCACAATGCC CAAGACTAGC 
CTGAAGGCAG CCACCATCAA GGCAGAGTTT GTGAGGGCAG AGACTCCATA CATGGTGATG 
AACCCTGAGA CAAAAGCACG GAGAGCTGGG CAGAGCGTGT CTCTGTGCTG TAAGGCCACA 
GGGAAGCCCA GGCCAGACAA GTATTTTTGG TATCATAATG ACACATTGCT GGATCCTTCC 
CTCTACAAGC ATGAGAGCAA GCTGGTGCTG AGGAAACTGC AGCAGCACCA GGCTGGGGAG 
TACTTTTGCA AGGCCCAGAG TGATGCTGGG GCTGTGAAGT CCAAGGTTGC CCAGCTGATT 
GTCATAGCAT CTGATGAGAC TCCTTGCAAC CCAGTTCCTG AGAGCTATCT TATCCGGCTG 
CCCCATGATT GCTTTCAGAA TGCCACCAAC TCCTTCTACT ATGACGTGGG ACGCTGCCCT 
GTTAAGACTT GTGCAGGGCA GCAGGATAAT GGGATCAGGT GCCGTGATGC TGTGCAGAAC 
TGCTGTGGCA TCTCCAAGAC AGAGGAAAGG GAGATCCAGT GCAGTGGCTA CACGCTACCC 
ACCAAGGTGG CCAAGGAGTG CAGCTGCCAG CGGTGTACGG AAACTCGGAG CATCGTGCGG 
GGCCGTGTCA GTGCTGCTGA CAATGGGGAG CCCATGCGCT TTGGCCATGT GTACATGGGG 
AACAGCCGTG TAAGCATGAC TGGCTACAAG GGCACTTTCA CCCTCCATGT CCCCCAGGAC 
ACTGAGAGGC TGGTGCTCAC ATTTGTGGAC AGGCTGCAGA AGTTTGTCAA CACCACCAAA 
GTGCTACCTT TCAACAAGAA GGGGAGTGCC GTGTTCCATG AAATCAAGAT GCTTCGTCGG 
AAAAAGCCCA TCACTTTGGA AGCCATGGAG ACCAACATCA TCCCCCTGGG GGAAGTGGTT 
GGTGAAGACC CCATGGCTGA ACTGGAGATT CCATCCAGGA GTTTCTACAG GCAGAATGGG 
GAGCCCTACA TAGGAAAAGT GAAGGCCAGT GTGACCTTCC TGGATCCCCG GAATATTTCC 
ACAGCCACAG CTGCCCAGAC TGACCTGAAC TTCATCAATG ACGAAGGAGA CACTTTCCCC 
CTTCGGACGT ATGGCATGTT CTCTGTGGAC TTCAGAGATG AGGTCACCTC AGAGCCACTT 
AATGCTGGCA AAGTGAAGGT CCACCTTGAC TCGACCCAGG TCAAGATGCC AGAGCACATA 
TCCACAGTGA AACTCTGGTC ACTCAATCCA GACACAGGGC TGTGGGAGGA GGAAGGTGAT 
TTCAAATTTG AAAATCAAAG GAGGAACAAA AGAGAAGACA GAACCTTCCT GGTGGGCAAC 
CTGGAGATTC GTGAGAGGAG GCTCTTTAAC CTGGATGTTC CTGAAAGCAG GCGGTGCTTT 
GTTAAGGTGA GGGCCTACCG GAGTGAGAGG TTCTTGCCTA GTGAGCAGAT CCAGGGGGTT 
GTGATCTCCG TGATTAACCT GGAGCCTAGA ACTGGCTTCT TGTCCAACCC TAGGGCCTGG 
GGCCGCTTTG ACAGTGTCAT CACAGGCCCC AACGGGGCCT GTGTGCCTGC CTTCTGTGAT 
GACCAGTCCC CTGATGCCTA CTCTGCCTAT GTCTTGGCAA GCCTGGCTGG GGAGGAACTG 
CAAGCAGTGG AGTCTTCTCC TAAATTCAAC CCAAATGCAA TTGGCGTCCC TCAGCCCTAT 
CTCAACAAGC TCAACTACCG TCGGACGGAC CATGAGGATC CACGGGTTAA AAAGACAGCT 
TTCCAGATTA GCATGGCCAA GCCAAGGCCC AACTCAGCTG AGGAGAGCAA TGGGCCCATC 
TATGCCTTTG AGAACCTCCG GGCATGTGAA GAGGCACCAC CCAGTGCAGC CCACTTCCGG 
TTCTACCAGA TTGAGGGGGA TCGATATGAC TACAACACAG TCCCCTTCAA CGAAGATGAC 
CCTATGAGCT GGACTGAAGA CTATCTGGCA TGGTGGCCAA AGCCGATGGA ATTCAGGGCC 
TGCTATATCA AGGTGAAGAT TGTGGGGCCA CTGGAAGTGA ATGTGCGATC CCGCAACATG 
GGGGGCACTC ATCGGCAGAC AGTGGGGAAG CTGTATGGAA TCCGAGATGT GAGGAGCACT 
CGGGACAGGG ACCAGCCCAA TGTCTCAGCT GCCTGTCTGG AGTTCAAGTG CAGTGGGATG 
CTCTATGATC AGGACCGTGT GGACCGCACC CTGGTGAAGG TCATCCCCCA GGGCAGCTGC 
CGTCGAGCCA GTGTGAACCC CATGCTGCAT GAGTACCTGG TCAACCACTT GCCACTTGCA 
GTCAACAACG ACACCAGTGA GTACACCATG CTGGCACCCT TGGACCCACT GGGCCACAAC 
TATGGCATCT ACACTGTCAC TGACCAGGAC CCTCGCACGG CCAAGGAGAT CGCGCTCGGC 
CGGTGCTTTG ATGGCACATC CGATGGCTCC TCCAGAATCA TGAAGAGCAA TGTGGGAGTA 
GCCCTCACCT TCAACTGTGT AGAGAGGCAA GTAGGCCGCC AGAGTGCCTT CCAGTACCTC 
CAAAGCACCC CAGCCCAGTC CCCTGCTGCA GGCACTGTCC AAGGAAGAGT GCCCTCGAGG 
AGGCAGCAGC GAGCGAGCAG GGGTGGCCAG CGCCAGGGTG GAGTGGTGGC CTCTCTGAGA 
TTTCCTAGAG TTGCTCAACA GCCCCTGATC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_017022679.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878168.1 Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

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CloneID	OHu85711
Clone ID Related Accession (Same CDS sequence)	XM_017022679.1
Accession Version	XM_017022679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cartilage intermediate layer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACCC AGTCAGTAAG AAGAGTCCAG CCTGGGAAGA AGAACCCCAG CATCTTTGCC 
AAGCCTGCCG ACACCCTGGA GAGCCCTGGT GAGTGGACAA CATGGTTCAA CATCGACTAC 
CCAGGCGGGA AGGGCGACTA TGAGCGGCTG GACGCCATTC GCTTCTACTA TGGGGACCGT 
GTATGTGCCC GTCCCCTGCG GCTAGAGGCT CGGACCACTG ACTGGACACC TGCGGGCAGC 
ACTGGCCAGG TGGTCCATGG TAGTCCCCGT GAGGGTTTCT GGTGCCTCAA CAGGGAGCAG 
CGGCCTGGCC AGAACTGCTC TAATTACACC GTACGCTTCC TCTGCCCACC AGGATCCCTG 
CGCCGAGACA CAGAGCGCAT CTGGAGCCCA TGGTCTCCCT GGAGCAAGTG CTCAGCTGCC 
TGTGGTCAGA CTGGGGTCCA GACTCGCACA CGCATTTGCT TGGCAGAGAT GGTGTCGCTG 
TGCAGTGAGG CCAGCGAAGA GGGTCAGCAC TGCATGGGCC AGGACTGTAC AGCCTGTGAC 
CTGACCTGCC CAATGGGCCA GGTGAATGCT GACTGTGATG CCTGCATGTG CCAGGACTTC 
ATGCTTCATG GGGCTGTCTC CCTTCCCGGA GGTGCCCCAG CCTCAGGGGC TGCTATCTAC 
CTCCTGACCA AGACGCCGAA GCTGCTGACC CAGACAGACA GTGATGGGAG ATTCCGAATC 
CCTGGCTTGT GCCCTGATGG CAAAAGCATC CTGAAGATCA CAAAGGTCAA GTTTGCCCCC 
ATTGTACTCA CAATGCCCAA GACTAGCCTG AAGGCAGCCA CCATCAAGGC AGAGTTTGTG 
AGGGCAGAGA CTCCATACAT GGTGATGAAC CCTGAGACAA AAGCACGGAG AGCTGGGCAG 
AGCGTGTCTC TGTGCTGTAA GGCCACAGGG AAGCCCAGGC CAGACAAGTA TTTTTGGTAT 
CATAATGACA CATTGCTGGA TCCTTCCCTC TACAAGCATG AGAGCAAGCT GGTGCTGAGG 
AAACTGCAGC AGCACCAGGC TGGGGAGTAC TTTTGCAAGG CCCAGAGTGA TGCTGGGGCT 
GTGAAGTCCA AGGTTGCCCA GCTGATTGTC ATAGCATCTG ATGAGACTCC TTGCAACCCA 
GTTCCTGAGA GCTATCTTAT CCGGCTGCCC CATGATTGCT TTCAGAATGC CACCAACTCC 
TTCTACTATG ACGTGGGACG CTGCCCTGTT AAGACTTGTG CAGGGCAGCA GGATAATGGG 
ATCAGGTGCC GTGATGCTGT GCAGAACTGC TGTGGCATCT CCAAGACAGA GGAAAGGGAG 
ATCCAGTGCA GTGGCTACAC GCTACCCACC AAGGTGGCCA AGGAGTGCAG CTGCCAGCGG 
TGTACGGAAA CTCGGAGCAT CGTGCGGGGC CGTGTCAGTG CTGCTGACAA TGGGGAGCCC 
ATGCGCTTTG GCCATGTGTA CATGGGGAAC AGCCGTGTAA GCATGACTGG CTACAAGGGC 
ACTTTCACCC TCCATGTCCC CCAGGACACT GAGAGGCTGG TGCTCACATT TGTGGACAGG 
CTGCAGAAGT TTGTCAACAC CACCAAAGTG CTACCTTTCA ACAAGAAGGG GAGTGCCGTG 
TTCCATGAAA TCAAGATGCT TCGTCGGAAA AAGCCCATCA CTTTGGAAGC CATGGAGACC 
AACATCATCC CCCTGGGGGA AGTGGTTGGT GAAGACCCCA TGGCTGAACT GGAGATTCCA 
TCCAGGAGTT TCTACAGGCA GAATGGGGAG CCCTACATAG GAAAAGTGAA GGCCAGTGTG 
ACCTTCCTGG ATCCCCGGAA TATTTCCACA GCCACAGCTG CCCAGACTGA CCTGAACTTC 
ATCAATGACG AAGGAGACAC TTTCCCCCTT CGGACGTATG GCATGTTCTC TGTGGACTTC 
AGAGATGAGG TCACCTCAGA GCCACTTAAT GCTGGCAAAG TGAAGGTCCA CCTTGACTCG 
ACCCAGGTCA AGATGCCAGA GCACATATCC ACAGTGAAAC TCTGGTCACT CAATCCAGAC 
ACAGGGCTGT GGGAGGAGGA AGGTGATTTC AAATTTGAAA ATCAAAGGAG GAACAAAAGA 
GAAGACAGAA CCTTCCTGGT GGGCAACCTG GAGATTCGTG AGAGGAGGCT CTTTAACCTG 
GATGTTCCTG AAAGCAGGCG GTGCTTTGTT AAGGTGAGGG CCTACCGGAG TGAGAGGTTC 
TTGCCTAGTG AGCAGATCCA GGGGGTTGTG ATCTCCGTGA TTAACCTGGA GCCTAGAACT 
GGCTTCTTGT CCAACCCTAG GGCCTGGGGC CGCTTTGACA GTGTCATCAC AGGCCCCAAC 
GGGGCCTGTG TGCCTGCCTT CTGTGATGAC CAGTCCCCTG ATGCCTACTC TGCCTATGTC 
TTGGCAAGCC TGGCTGGGGA GGAACTGCAA GCAGTGGAGT CTTCTCCTAA ATTCAACCCA 
AATGCAATTG GCGTCCCTCA GCCCTATCTC AACAAGCTCA ACTACCGTCG GACGGACCAT 
GAGGATCCAC GGGTTAAAAA GACAGCTTTC CAGATTAGCA TGGCCAAGCC AAGGCCCAAC 
TCAGCTGAGG AGAGCAATGG GCCCATCTAT GCCTTTGAGA ACCTCCGGGC ATGTGAAGAG 
GCACCACCCA GTGCAGCCCA CTTCCGGTTC TACCAGATTG AGGGGGATCG ATATGACTAC 
AACACAGTCC CCTTCAACGA AGATGACCCT ATGAGCTGGA CTGAAGACTA TCTGGCATGG 
TGGCCAAAGC CGATGGAATT CAGGGCCTGC TATATCAAGG TGAAGATTGT GGGGCCACTG 
GAAGTGAATG TGCGATCCCG CAACATGGGG GGCACTCATC GGCAGACAGT GGGGAAGCTG 
TATGGAATCC GAGATGTGAG GAGCACTCGG GACAGGGACC AGCCCAATGT CTCAGCTGCC 
TGTCTGGAGT TCAAGTGCAG TGGGATGCTC TATGATCAGG ACCGTGTGGA CCGCACCCTG 
GTGAAGGTCA TCCCCCAGGG CAGCTGCCGT CGAGCCAGTG TGAACCCCAT GCTGCATGAG 
TACCTGGTCA ACCACTTGCC ACTTGCAGTC AACAACGACA CCAGTGAGTA CACCATGCTG 
GCACCCTTGG ACCCACTGGG CCACAACTAT GGCATCTACA CTGTCACTGA CCAGGACCCT 
CGCACGGCCA AGGAGATCGC GCTCGGCCGG TGCTTTGATG GCACATCCGA TGGCTCCTCC 
AGAATCATGA AGAGCAATGT GGGAGTAGCC CTCACCTTCA ACTGTGTAGA GAGGCAAGTA 
GGCCGCCAGA GTGCCTTCCA GTACCTCCAA AGCACCCCAG CCCAGTCCCC TGCTGCAGGC 
ACTGTCCAAG GAAGAGTGCC CTCGAGGAGG CAGCAGCGAG CGAGCAGGGG TGGCCAGCGC 
CAGGGTGGAG TGGTGGCCTC TCTGAGATTT CCTAGAGTTG CTCAACAGCC CCTGATCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878168.1
CDS	370..3852
Translation	MLTQSVRRVQPGKKNPSIFAKPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSKCSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDACMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILKITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATGKPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFGHVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRRKKPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASVTFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHLDSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN

Target ORF information:

RefSeq Version	XM_017022679.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022679.1

ORF Insert Sequence:

ATGCTCACCC AGTCAGTAAG AAGAGTCCAG CCTGGGAAGA AGAACCCCAG CATCTTTGCC 
AAGCCTGCCG ACACCCTGGA GAGCCCTGGT GAGTGGACAA CATGGTTCAA CATCGACTAC 
CCAGGCGGGA AGGGCGACTA TGAGCGGCTG GACGCCATTC GCTTCTACTA TGGGGACCGT 
GTATGTGCCC GTCCCCTGCG GCTAGAGGCT CGGACCACTG ACTGGACACC TGCGGGCAGC 
ACTGGCCAGG TGGTCCATGG TAGTCCCCGT GAGGGTTTCT GGTGCCTCAA CAGGGAGCAG 
CGGCCTGGCC AGAACTGCTC TAATTACACC GTACGCTTCC TCTGCCCACC AGGATCCCTG 
CGCCGAGACA CAGAGCGCAT CTGGAGCCCA TGGTCTCCCT GGAGCAAGTG CTCAGCTGCC 
TGTGGTCAGA CTGGGGTCCA GACTCGCACA CGCATTTGCT TGGCAGAGAT GGTGTCGCTG 
TGCAGTGAGG CCAGCGAAGA GGGTCAGCAC TGCATGGGCC AGGACTGTAC AGCCTGTGAC 
CTGACCTGCC CAATGGGCCA GGTGAATGCT GACTGTGATG CCTGCATGTG CCAGGACTTC 
ATGCTTCATG GGGCTGTCTC CCTTCCCGGA GGTGCCCCAG CCTCAGGGGC TGCTATCTAC 
CTCCTGACCA AGACGCCGAA GCTGCTGACC CAGACAGACA GTGATGGGAG ATTCCGAATC 
CCTGGCTTGT GCCCTGATGG CAAAAGCATC CTGAAGATCA CAAAGGTCAA GTTTGCCCCC 
ATTGTACTCA CAATGCCCAA GACTAGCCTG AAGGCAGCCA CCATCAAGGC AGAGTTTGTG 
AGGGCAGAGA CTCCATACAT GGTGATGAAC CCTGAGACAA AAGCACGGAG AGCTGGGCAG 
AGCGTGTCTC TGTGCTGTAA GGCCACAGGG AAGCCCAGGC CAGACAAGTA TTTTTGGTAT 
CATAATGACA CATTGCTGGA TCCTTCCCTC TACAAGCATG AGAGCAAGCT GGTGCTGAGG 
AAACTGCAGC AGCACCAGGC TGGGGAGTAC TTTTGCAAGG CCCAGAGTGA TGCTGGGGCT 
GTGAAGTCCA AGGTTGCCCA GCTGATTGTC ATAGCATCTG ATGAGACTCC TTGCAACCCA 
GTTCCTGAGA GCTATCTTAT CCGGCTGCCC CATGATTGCT TTCAGAATGC CACCAACTCC 
TTCTACTATG ACGTGGGACG CTGCCCTGTT AAGACTTGTG CAGGGCAGCA GGATAATGGG 
ATCAGGTGCC GTGATGCTGT GCAGAACTGC TGTGGCATCT CCAAGACAGA GGAAAGGGAG 
ATCCAGTGCA GTGGCTACAC GCTACCCACC AAGGTGGCCA AGGAGTGCAG CTGCCAGCGG 
TGTACGGAAA CTCGGAGCAT CGTGCGGGGC CGTGTCAGTG CTGCTGACAA TGGGGAGCCC 
ATGCGCTTTG GCCATGTGTA CATGGGGAAC AGCCGTGTAA GCATGACTGG CTACAAGGGC 
ACTTTCACCC TCCATGTCCC CCAGGACACT GAGAGGCTGG TGCTCACATT TGTGGACAGG 
CTGCAGAAGT TTGTCAACAC CACCAAAGTG CTACCTTTCA ACAAGAAGGG GAGTGCCGTG 
TTCCATGAAA TCAAGATGCT TCGTCGGAAA AAGCCCATCA CTTTGGAAGC CATGGAGACC 
AACATCATCC CCCTGGGGGA AGTGGTTGGT GAAGACCCCA TGGCTGAACT GGAGATTCCA 
TCCAGGAGTT TCTACAGGCA GAATGGGGAG CCCTACATAG GAAAAGTGAA GGCCAGTGTG 
ACCTTCCTGG ATCCCCGGAA TATTTCCACA GCCACAGCTG CCCAGACTGA CCTGAACTTC 
ATCAATGACG AAGGAGACAC TTTCCCCCTT CGGACGTATG GCATGTTCTC TGTGGACTTC 
AGAGATGAGG TCACCTCAGA GCCACTTAAT GCTGGCAAAG TGAAGGTCCA CCTTGACTCG 
ACCCAGGTCA AGATGCCAGA GCACATATCC ACAGTGAAAC TCTGGTCACT CAATCCAGAC 
ACAGGGCTGT GGGAGGAGGA AGGTGATTTC AAATTTGAAA ATCAAAGGAG GAACAAAAGA 
GAAGACAGAA CCTTCCTGGT GGGCAACCTG GAGATTCGTG AGAGGAGGCT CTTTAACCTG 
GATGTTCCTG AAAGCAGGCG GTGCTTTGTT AAGGTGAGGG CCTACCGGAG TGAGAGGTTC 
TTGCCTAGTG AGCAGATCCA GGGGGTTGTG ATCTCCGTGA TTAACCTGGA GCCTAGAACT 
GGCTTCTTGT CCAACCCTAG GGCCTGGGGC CGCTTTGACA GTGTCATCAC AGGCCCCAAC 
GGGGCCTGTG TGCCTGCCTT CTGTGATGAC CAGTCCCCTG ATGCCTACTC TGCCTATGTC 
TTGGCAAGCC TGGCTGGGGA GGAACTGCAA GCAGTGGAGT CTTCTCCTAA ATTCAACCCA 
AATGCAATTG GCGTCCCTCA GCCCTATCTC AACAAGCTCA ACTACCGTCG GACGGACCAT 
GAGGATCCAC GGGTTAAAAA GACAGCTTTC CAGATTAGCA TGGCCAAGCC AAGGCCCAAC 
TCAGCTGAGG AGAGCAATGG GCCCATCTAT GCCTTTGAGA ACCTCCGGGC ATGTGAAGAG 
GCACCACCCA GTGCAGCCCA CTTCCGGTTC TACCAGATTG AGGGGGATCG ATATGACTAC 
AACACAGTCC CCTTCAACGA AGATGACCCT ATGAGCTGGA CTGAAGACTA TCTGGCATGG 
TGGCCAAAGC CGATGGAATT CAGGGCCTGC TATATCAAGG TGAAGATTGT GGGGCCACTG 
GAAGTGAATG TGCGATCCCG CAACATGGGG GGCACTCATC GGCAGACAGT GGGGAAGCTG 
TATGGAATCC GAGATGTGAG GAGCACTCGG GACAGGGACC AGCCCAATGT CTCAGCTGCC 
TGTCTGGAGT TCAAGTGCAG TGGGATGCTC TATGATCAGG ACCGTGTGGA CCGCACCCTG 
GTGAAGGTCA TCCCCCAGGG CAGCTGCCGT CGAGCCAGTG TGAACCCCAT GCTGCATGAG 
TACCTGGTCA ACCACTTGCC ACTTGCAGTC AACAACGACA CCAGTGAGTA CACCATGCTG 
GCACCCTTGG ACCCACTGGG CCACAACTAT GGCATCTACA CTGTCACTGA CCAGGACCCT 
CGCACGGCCA AGGAGATCGC GCTCGGCCGG TGCTTTGATG GCACATCCGA TGGCTCCTCC 
AGAATCATGA AGAGCAATGT GGGAGTAGCC CTCACCTTCA ACTGTGTAGA GAGGCAAGTA 
GGCCGCCAGA GTGCCTTCCA GTACCTCCAA AGCACCCCAG CCCAGTCCCC TGCTGCAGGC 
ACTGTCCAAG GAAGAGTGCC CTCGAGGAGG CAGCAGCGAG CGAGCAGGGG TGGCCAGCGC 
CAGGGTGGAG TGGTGGCCTC TCTGAGATTT CCTAGAGTTG CTCAACAGCC CCTGATCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( NM_003613.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003604.3 Homo sapiens cartilage intermediate layer protein (CILP), mRNA.

Price & Availability↑

CloneID	OHu19478
Clone ID Related Accession (Same CDS sequence)	NM_003613.3
Accession Version	NM_003613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	cartilage intermediate layer protein 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB071945.1, AK313352.1, BC035776.1, AC068213.7 and CA503413.1. This sequence is a reference standard in the RefSeqGene project. On Jun 24, 2008 this sequence version replaced NM_003613.2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358904.1, AF035408.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467146, SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGGGA CCAAGGCCTG GGTGTTCTCC TTCCTGGTCC TGGAAGTCAC ATCTGTGTTG 
GGGAGACAGA CGATGCTCAC CCAGTCAGTA AGAAGAGTCC AGCCTGGGAA GAAGAACCCC 
AGCATCTTTG CCAAGCCTGC CGACACCCTG GAGAGCCCTG GTGAGTGGAC AACATGGTTC 
AACATCGACT ACCCAGGCGG GAAGGGCGAC TATGAGCGGC TGGACGCCAT TCGCTTCTAC 
TATGGGGACC GTGTATGTGC CCGTCCCCTG CGGCTAGAGG CTCGGACCAC TGACTGGACA 
CCTGCGGGCA GCACTGGCCA GGTGGTCCAT GGTAGTCCCC GTGAGGGTTT CTGGTGCCTC 
AACAGGGAGC AGCGGCCTGG CCAGAACTGC TCTAATTACA CCGTACGCTT CCTCTGCCCA 
CCAGGATCCC TGCGCCGAGA CACAGAGCGC ATCTGGAGCC CATGGTCTCC CTGGAGCAAG 
TGCTCAGCTG CCTGTGGTCA GACTGGGGTC CAGACTCGCA CACGCATTTG CTTGGCAGAG 
ATGGTGTCGC TGTGCAGTGA GGCCAGCGAA GAGGGTCAGC ACTGCATGGG CCAGGACTGT 
ACAGCCTGTG ACCTGACCTG CCCAATGGGC CAGGTGAATG CTGACTGTGA TGCCTGCATG 
TGCCAGGACT TCATGCTTCA TGGGGCTGTC TCCCTTCCCG GAGGTGCCCC AGCCTCAGGG 
GCTGCTATCT ACCTCCTGAC CAAGACGCCG AAGCTGCTGA CCCAGACAGA CAGTGATGGG 
AGATTCCGAA TCCCTGGCTT GTGCCCTGAT GGCAAAAGCA TCCTGAAGAT CACAAAGGTC 
AAGTTTGCCC CCATTGTACT CACAATGCCC AAGACTAGCC TGAAGGCAGC CACCATCAAG 
GCAGAGTTTG TGAGGGCAGA GACTCCATAC ATGGTGATGA ACCCTGAGAC AAAAGCACGG 
AGAGCTGGGC AGAGCGTGTC TCTGTGCTGT AAGGCCACAG GGAAGCCCAG GCCAGACAAG 
TATTTTTGGT ATCATAATGA CACATTGCTG GATCCTTCCC TCTACAAGCA TGAGAGCAAG 
CTGGTGCTGA GGAAACTGCA GCAGCACCAG GCTGGGGAGT ACTTTTGCAA GGCCCAGAGT 
GATGCTGGGG CTGTGAAGTC CAAGGTTGCC CAGCTGATTG TCATAGCATC TGATGAGACT 
CCTTGCAACC CAGTTCCTGA GAGCTATCTT ATCCGGCTGC CCCATGATTG CTTTCAGAAT 
GCCACCAACT CCTTCTACTA TGACGTGGGA CGCTGCCCTG TTAAGACTTG TGCAGGGCAG 
CAGGATAATG GGATCAGGTG CCGTGATGCT GTGCAGAACT GCTGTGGCAT CTCCAAGACA 
GAGGAAAGGG AGATCCAGTG CAGTGGCTAC ACGCTACCCA CCAAGGTGGC CAAGGAGTGC 
AGCTGCCAGC GGTGTACGGA AACTCGGAGC ATCGTGCGGG GCCGTGTCAG TGCTGCTGAC 
AATGGGGAGC CCATGCGCTT TGGCCATGTG TACATGGGGA ACAGCCGTGT AAGCATGACT 
GGCTACAAGG GCACTTTCAC CCTCCATGTC CCCCAGGACA CTGAGAGGCT GGTGCTCACA 
TTTGTGGACA GGCTGCAGAA GTTTGTCAAC ACCACCAAAG TGCTACCTTT CAACAAGAAG 
GGGAGTGCCG TGTTCCATGA AATCAAGATG CTTCGTCGGA AAGAGCCCAT CACTTTGGAA 
GCCATGGAGA CCAACATCAT CCCCCTGGGG GAAGTGGTTG GTGAAGACCC CATGGCTGAA 
CTGGAGATTC CATCCAGGAG TTTCTACAGG CAGAATGGGG AGCCCTACAT AGGAAAAGTG 
AAGGCCAGTG TGACCTTCCT GGATCCCCGG AATATTTCCA CAGCCACAGC TGCCCAGACT 
GACCTGAACT TCATCAATGA CGAAGGAGAC ACTTTCCCCC TTCGGACGTA TGGCATGTTC 
TCTGTGGACT TCAGAGATGA GGTCACCTCA GAGCCACTTA ATGCTGGCAA AGTGAAGGTC 
CACCTTGACT CGACCCAGGT CAAGATGCCA GAGCACATAT CCACAGTGAA ACTCTGGTCA 
CTCAATCCAG ACACAGGGCT GTGGGAGGAG GAAGGTGATT TCAAATTTGA AAATCAAAGG 
AGGAACAAAA GAGAAGACAG AACCTTCCTG GTGGGCAACC TGGAGATTCG TGAGAGGAGG 
CTCTTTAACC TGGATGTTCC TGAAAGCAGG CGGTGCTTTG TTAAGGTGAG GGCCTACCGG 
AGTGAGAGGT TCTTGCCTAG TGAGCAGATC CAGGGGGTTG TGATCTCCGT GATTAACCTG 
GAGCCTAGAA CTGGCTTCTT GTCCAACCCT AGGGCCTGGG GCCGCTTTGA CAGTGTCATC 
ACAGGCCCCA ACGGGGCCTG TGTGCCTGCC TTCTGTGATG ACCAGTCCCC TGATGCCTAC 
TCTGCCTATG TCTTGGCAAG CCTGGCTGGG GAGGAACTGC AAGCAGTGGA GTCTTCTCCT 
AAATTCAACC CAAATGCAAT TGGCGTCCCT CAGCCCTATC TCAACAAGCT CAACTACCGT 
CGGACGGACC ATGAGGATCC ACGGGTTAAA AAGACAGCTT TCCAGATTAG CATGGCCAAG 
CCAAGGCCCA ACTCAGCTGA GGAGAGCAAT GGGCCCATCT ATGCCTTTGA GAACCTCCGG 
GCATGTGAAG AGGCACCACC CAGTGCAGCC CACTTCCGGT TCTACCAGAT TGAGGGGGAT 
CGATATGACT ACAACACAGT CCCCTTCAAC GAAGATGACC CTATGAGCTG GACTGAAGAC 
TATCTGGCAT GGTGGCCAAA GCCGATGGAA TTCAGGGCCT GCTATATCAA GGTGAAGATT 
GTGGGGCCAC TGGAAGTGAA TGTGCGATCC CGCAACATGG GGGGCACTCA TCGGCAGACA 
GTGGGGAAGC TGTATGGAAT CCGAGATGTG AGGAGCACTC GGGACAGGGA CCAGCCCAAT 
GTCTCAGCTG CCTGTCTGGA GTTCAAGTGC AGTGGGATGC TCTATGATCA GGACCGTGTG 
GACCGCACCC TGGTGAAGGT CATCCCCCAG GGCAGCTGCC GTCGAGCCAG TGTGAACCCC 
ATGCTGCATG AGTACCTGGT CAACCACTTG CCACTTGCAG TCAACAACGA CACCAGTGAG 
TACACCATGC TGGCACCCTT GGACCCACTG GGCCACAACT ATGGCATCTA CACTGTCACT 
GACCAGGACC CTCGCACGGC CAAGGAGATC GCGCTCGGCC GGTGCTTTGA TGGCACATCC 
GATGGCTCCT CCAGAATCAT GAAGAGCAAT GTGGGAGTAG CCCTCACCTT CAACTGTGTA 
GAGAGGCAAG TAGGCCGCCA GAGTGCCTTC CAGTACCTCC AAAGCACCCC AGCCCAGTCC 
CCTGCTGCAG GCACTGTCCA AGGAAGAGTG CCCTCGAGGA GGCAGCAGCG AGCGAGCAGG 
GGTGGCCAGC GCCAGGGTGG AGTGGTGGCC TCTCTGAGAT TTCCTAGAGT TGCTCAACAG 
CCCCTGATCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003604.3
CDS	182..3736
Translation	MVGTKAWVFSFLVLEVTSVLGRQTMLTQSVRRVQPGKKNPSIFAKPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSKCSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDACMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILKITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATGKPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFGHVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASVTFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHLDSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN

Target ORF information:

RefSeq Version	NM_003613.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cartilage intermediate layer protein (CILP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003613.3

ORF Insert Sequence:

ATGGTGGGGA CCAAGGCCTG GGTGTTCTCC TTCCTGGTCC TGGAAGTCAC ATCTGTGTTG 
GGGAGACAGA CGATGCTCAC CCAGTCAGTA AGAAGAGTCC AGCCTGGGAA GAAGAACCCC 
AGCATCTTTG CCAAGCCTGC CGACACCCTG GAGAGCCCTG GTGAGTGGAC AACATGGTTC 
AACATCGACT ACCCAGGCGG GAAGGGCGAC TATGAGCGGC TGGACGCCAT TCGCTTCTAC 
TATGGGGACC GTGTATGTGC CCGTCCCCTG CGGCTAGAGG CTCGGACCAC TGACTGGACA 
CCTGCGGGCA GCACTGGCCA GGTGGTCCAT GGTAGTCCCC GTGAGGGTTT CTGGTGCCTC 
AACAGGGAGC AGCGGCCTGG CCAGAACTGC TCTAATTACA CCGTACGCTT CCTCTGCCCA 
CCAGGATCCC TGCGCCGAGA CACAGAGCGC ATCTGGAGCC CATGGTCTCC CTGGAGCAAG 
TGCTCAGCTG CCTGTGGTCA GACTGGGGTC CAGACTCGCA CACGCATTTG CTTGGCAGAG 
ATGGTGTCGC TGTGCAGTGA GGCCAGCGAA GAGGGTCAGC ACTGCATGGG CCAGGACTGT 
ACAGCCTGTG ACCTGACCTG CCCAATGGGC CAGGTGAATG CTGACTGTGA TGCCTGCATG 
TGCCAGGACT TCATGCTTCA TGGGGCTGTC TCCCTTCCCG GAGGTGCCCC AGCCTCAGGG 
GCTGCTATCT ACCTCCTGAC CAAGACGCCG AAGCTGCTGA CCCAGACAGA CAGTGATGGG 
AGATTCCGAA TCCCTGGCTT GTGCCCTGAT GGCAAAAGCA TCCTGAAGAT CACAAAGGTC 
AAGTTTGCCC CCATTGTACT CACAATGCCC AAGACTAGCC TGAAGGCAGC CACCATCAAG 
GCAGAGTTTG TGAGGGCAGA GACTCCATAC ATGGTGATGA ACCCTGAGAC AAAAGCACGG 
AGAGCTGGGC AGAGCGTGTC TCTGTGCTGT AAGGCCACAG GGAAGCCCAG GCCAGACAAG 
TATTTTTGGT ATCATAATGA CACATTGCTG GATCCTTCCC TCTACAAGCA TGAGAGCAAG 
CTGGTGCTGA GGAAACTGCA GCAGCACCAG GCTGGGGAGT ACTTTTGCAA GGCCCAGAGT 
GATGCTGGGG CTGTGAAGTC CAAGGTTGCC CAGCTGATTG TCATAGCATC TGATGAGACT 
CCTTGCAACC CAGTTCCTGA GAGCTATCTT ATCCGGCTGC CCCATGATTG CTTTCAGAAT 
GCCACCAACT CCTTCTACTA TGACGTGGGA CGCTGCCCTG TTAAGACTTG TGCAGGGCAG 
CAGGATAATG GGATCAGGTG CCGTGATGCT GTGCAGAACT GCTGTGGCAT CTCCAAGACA 
GAGGAAAGGG AGATCCAGTG CAGTGGCTAC ACGCTACCCA CCAAGGTGGC CAAGGAGTGC 
AGCTGCCAGC GGTGTACGGA AACTCGGAGC ATCGTGCGGG GCCGTGTCAG TGCTGCTGAC 
AATGGGGAGC CCATGCGCTT TGGCCATGTG TACATGGGGA ACAGCCGTGT AAGCATGACT 
GGCTACAAGG GCACTTTCAC CCTCCATGTC CCCCAGGACA CTGAGAGGCT GGTGCTCACA 
TTTGTGGACA GGCTGCAGAA GTTTGTCAAC ACCACCAAAG TGCTACCTTT CAACAAGAAG 
GGGAGTGCCG TGTTCCATGA AATCAAGATG CTTCGTCGGA AAGAGCCCAT CACTTTGGAA 
GCCATGGAGA CCAACATCAT CCCCCTGGGG GAAGTGGTTG GTGAAGACCC CATGGCTGAA 
CTGGAGATTC CATCCAGGAG TTTCTACAGG CAGAATGGGG AGCCCTACAT AGGAAAAGTG 
AAGGCCAGTG TGACCTTCCT GGATCCCCGG AATATTTCCA CAGCCACAGC TGCCCAGACT 
GACCTGAACT TCATCAATGA CGAAGGAGAC ACTTTCCCCC TTCGGACGTA TGGCATGTTC 
TCTGTGGACT TCAGAGATGA GGTCACCTCA GAGCCACTTA ATGCTGGCAA AGTGAAGGTC 
CACCTTGACT CGACCCAGGT CAAGATGCCA GAGCACATAT CCACAGTGAA ACTCTGGTCA 
CTCAATCCAG ACACAGGGCT GTGGGAGGAG GAAGGTGATT TCAAATTTGA AAATCAAAGG 
AGGAACAAAA GAGAAGACAG AACCTTCCTG GTGGGCAACC TGGAGATTCG TGAGAGGAGG 
CTCTTTAACC TGGATGTTCC TGAAAGCAGG CGGTGCTTTG TTAAGGTGAG GGCCTACCGG 
AGTGAGAGGT TCTTGCCTAG TGAGCAGATC CAGGGGGTTG TGATCTCCGT GATTAACCTG 
GAGCCTAGAA CTGGCTTCTT GTCCAACCCT AGGGCCTGGG GCCGCTTTGA CAGTGTCATC 
ACAGGCCCCA ACGGGGCCTG TGTGCCTGCC TTCTGTGATG ACCAGTCCCC TGATGCCTAC 
TCTGCCTATG TCTTGGCAAG CCTGGCTGGG GAGGAACTGC AAGCAGTGGA GTCTTCTCCT 
AAATTCAACC CAAATGCAAT TGGCGTCCCT CAGCCCTATC TCAACAAGCT CAACTACCGT 
CGGACGGACC ATGAGGATCC ACGGGTTAAA AAGACAGCTT TCCAGATTAG CATGGCCAAG 
CCAAGGCCCA ACTCAGCTGA GGAGAGCAAT GGGCCCATCT ATGCCTTTGA GAACCTCCGG 
GCATGTGAAG AGGCACCACC CAGTGCAGCC CACTTCCGGT TCTACCAGAT TGAGGGGGAT 
CGATATGACT ACAACACAGT CCCCTTCAAC GAAGATGACC CTATGAGCTG GACTGAAGAC 
TATCTGGCAT GGTGGCCAAA GCCGATGGAA TTCAGGGCCT GCTATATCAA GGTGAAGATT 
GTGGGGCCAC TGGAAGTGAA TGTGCGATCC CGCAACATGG GGGGCACTCA TCGGCAGACA 
GTGGGGAAGC TGTATGGAAT CCGAGATGTG AGGAGCACTC GGGACAGGGA CCAGCCCAAT 
GTCTCAGCTG CCTGTCTGGA GTTCAAGTGC AGTGGGATGC TCTATGATCA GGACCGTGTG 
GACCGCACCC TGGTGAAGGT CATCCCCCAG GGCAGCTGCC GTCGAGCCAG TGTGAACCCC 
ATGCTGCATG AGTACCTGGT CAACCACTTG CCACTTGCAG TCAACAACGA CACCAGTGAG 
TACACCATGC TGGCACCCTT GGACCCACTG GGCCACAACT ATGGCATCTA CACTGTCACT 
GACCAGGACC CTCGCACGGC CAAGGAGATC GCGCTCGGCC GGTGCTTTGA TGGCACATCC 
GATGGCTCCT CCAGAATCAT GAAGAGCAAT GTGGGAGTAG CCCTCACCTT CAACTGTGTA 
GAGAGGCAAG TAGGCCGCCA GAGTGCCTTC CAGTACCTCC AAAGCACCCC AGCCCAGTCC 
CCTGCTGCAG GCACTGTCCA AGGAAGAGTG CCCTCGAGGA GGCAGCAGCG AGCGAGCAGG 
GGTGGCCAGC GCCAGGGTGG AGTGGTGGCC TCTCTGAGAT TTCCTAGAGT TGCTCAACAG 
CCCCTGATCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional polymorphisms in asporin and CILP together with joint loading predispose to hand osteoarthritis.
BMC genetics18(1)108(2017 Dec)
Taipale M,Solovieva S,Leino-Arjas P,M?nnikk? M

Cartilage intermediate layer protein 1 (CILP1): A novel mediator of cardiac extracellular matrix remodelling.
Scientific reports7(1)16042(2017 Nov)
van Nieuwenhoven FA,Munts C,Op't Veld RC,Gonz?lez A,D?ez J,Heymans S,Schroen B,van Bilsen M

Association Between Cartilage Intermediate Layer Protein and Degeneration of Intervertebral Disc: A Meta-analysis.
Spine41(20)E1244-E1248(2016 Oct)
Wang W,Hao J,Zheng S,Xiao X,Wen Y,He A,Guo X,Zhang F

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

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CILP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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