LOC105228957 cDNA ORF clone, Bactrocera dorsalis(oriental fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105228957
Entrez Gene ID	105228957
Full Name	DNA replication licensing factor MCM4
Synonyms	Mcm4
Gene Type	protein-coding
Organism	Bactrocera dorsalis(oriental fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011208986.2	XP_011207288.1	DNA replication licensing factor MCM4
XM_011208986.3	XP_011207288.1	DNA replication licensing factor MCM4

Pathways from BioSystems
Gene Ontology

The following LOC105228957 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105228957 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa129677	XM_011208986.3 Latest version!	Bactrocera dorsalis DNA replication licensing factor MCM4 (LOC105228957), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa129677	XM_011208986.2	Bactrocera dorsalis DNA replication licensing factor MCM4 (LOC105228957), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105228957 ( XM_011208986.2 ) cDNA ORF clone, Bactrocera dorsalis(oriental fruit fly) -> XP_011207288.1 Bactrocera dorsalis DNA replication licensing factor MCM4 (LOC105228957), mRNA.

Price & Availability↑

CloneID	OBa129677
Clone ID Related Accession (Same CDS sequence)	XM_011208986.2 , XM_011208986.3
Accession Version	XM_011208986.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-16
Organism	Bactrocera dorsalis(oriental fruit fly)
Product	DNA replication licensing factor MCM4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011876376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 17, 2017 this sequence version replaced XM_011208986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera dorsalis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGTC CCGCTCGCAC ACCAGAAGGT GGGGTAACCC CAAGAGGTCA AGGCGAACGT 
ACTCCAACGC GAAACCCTGT AGTTCAGGAT GCAACAGATA CACCGATGCG TATGGGTCCA 
ACAACTCAAC GTCAGAGCCA ACAGCCATCG GAAATTAGTT TGCCTCCAAC ATCACCGGGC 
GGTTTGAGTT TACCAGCAAC AAGTCCAGCC CGTGCGCTGG GCATGAGTGA AATTGATCTA 
AGTTCCCCAT TGAACTATGG TACACCCAGC TCAATTGGTT CTATTCGTAC ACCACGTTCG 
GGTATTCGTG GTACACCTTT GCGAGCACGT CCCGATATAC GCACAGATAA AAGGATGCGT 
CAAGTGACAA TTGGTGGTTC TGCACCCGGT TTGGATCCAA TTGCTGAGAA GCCCTCAGAA 
GGAACTGATA CTGTGTCTGA GTCGTCATCA ACACCGCAGA TGGTTGTTTG GGGCACCAAT 
GTTGTGGTGC ATCAATGTAA AACAAAATTC AAAGTTTTTC TTATGCGCTA CATTGATCCT 
GACGTAGAGC GCGACGAAAT TTCTGAAAAT ATAGATATAA ATCAGCCAAT ATATATGCAA 
AAGTTGGAAG AGATTCACAC TCTAGAAGAG CCTTACCTAA ATGTGAACTG CGCACATTTG 
AAAACATTTG ATGAAGCACT CTATCGTCAG TTGATTTGTT ACCCTCAAGA AGTGATCCCA 
GCTTTCGATA TGGCAGTAAA TGAAATGTTT TTCGAACGAT ACCCAGCAGC TGTATTAGAG 
CATCAGATTC AAGTACGTCC CTTTAATGCG GATAAGACAC GAAACATGCG TTCACTCAAC 
CCTGAGGATA TGGATCAGTT GTTGAGTATT AGTGGAATGG TAATACGAAC ATCTGGTGTT 
ATTCCGGAAA TGCGTGAAGC TTTCTTCAAA TGTATAATAT GTGCTTTCTG CACCGCAGTT 
GAAGTCGACA GAGGACGCAT TGCTCAACCA ACCCTCTGTA CAAATTGTAA TACAAATCAT 
TGTTTCCGAT TGGTGCACAA TCGTTCAGAA TTTACAGATA AACAACTAAT TAAACTGCAA 
GAGTCGCCGG ACGACATGGC TGCCGGCCAA ACACCACACA ATGTGCTTCT ATATGCTCAC 
AATGATTTAG TGGATAAGGT TCAGCCTGGT GATCGTGTCA CCGTAACTGG TATATATCGC 
GCTGTCCCAT TGAAGGATAA GGGGCGGAAT ATTAAGAGCG TTTACAAAAC ACATGTTGAC 
GTAGTGCATT ATCGGAAAGT TGACAGCAAC CGTTTGTACG AAGAGGAAGA AGGAAAAATG 
CATATTTTTA CACCAGAGCG TGTGGAATTG TTACATTTAC TCTCACAGAA ACCAGATATA 
TACGATCGTT TATCCAGAGC CATTGCTCCT TCCATTTATG AAAATGATGA CATCAAGAAA 
GGTATTTTAT TACAATTGTT TGGTGGTACA AAGAAAAAAC ATTCAACTTT GGGCAGACAG 
AATTTCAGAG CGGAAATTCA CTTGCTTTTG TGTGGTGACC CCGGTACATC AAAGTCTCAG 
TTGCTACAAT ATGTTTTCAA TTTAGTACCG CGCTCACAGT ACACTTCGGG TAGAGGATCT 
TCGGCAGTTG GTTTAACTGC TTATGTTACA AAAGATCCGG AAACGCGACA ACTAGTGTTG 
CAAACCGGTG CACTGGTATT GGCTGATAAT GGTGTTTGCT GCATCGATGA ATTTGATAAA 
ATGAATGATT CCACGCGCAG TGTTCTGCAT GAAGTCATGG AACAGCAGAC CCTAAGTATT 
GCCAAGGCTG GTATCATTTG TCAGCTTAAC GCACGTACCT CTATTTTGGC TGCTGCTAAT 
CCAGCTGAAT CGCAGTGGAA TAAGAAAAAG AATATAATCG ACAATGTACA ATTGCCACAT 
ACACTCCTGT CAAGATTTGA TTTAATTTTC CTTGTACTGG ATCCACAAGA TGAGCAATTT 
GACCGCCGTT TGGCAAATCA TCTTGTATCG CTTTATTATG TGACTCGTCA CGAAGAAGAA 
GATACCATGT TTGACGTTAG TGTACTACGT GACTACATTG CTTATGCTCG TGAACACTGC 
TCTCCTACTT TATCGGAGGA AGCTCAACAA CGCCTCATTC AAGCATATGT TGATATGCGC 
AAAGTTGGTG CACGTCGCGG TCAAATATCA GCGTATCCCA GACAGCTGGA AAGTTTGATA 
CGTTTATCGG AAGCACATGC TAAAGTTCGC TTGAGTAATG TAGTAACTGT AGAGGATGTA 
GAAGAAGCTT GGAGATTACA CCGTGAAGCT TTGAAACAAT CTGCCACCGA TCCACTTTCT 
GGTAAGATAG ATGTGGGTAT TCTTACAACA GGTTTGTCGA CTGCTGCACG TAAGAAACGA 
GCTGATCTCG TATTAGCTAT TAAGGAAAAC CTTAAAAAGA AAGGCAAAGT GCCAACTGTT 
CCGTATCAGA AACTATTTAA AGAAGTCAAA GATGCCTCAC AAATTCTTAT TACACGCGAA 
CAATTCGAGG ATGCGCTCAA AGAAATTCAA GATGAAGGCG CCATTGTTGT GATGGGTAAA 
AATACCATCA GAATCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011207288.1
CDS	128..2728
Translation	MSSPARTPEGGVTPRGQGERTPTRNPVVQDATDTPMRMGPTTQRQSQQPSEISLPPTSPGGLSLPATSPARALGMSEIDLSSPLNYGTPSSIGSIRTPRSGIRGTPLRARPDIRTDKRMRQVTIGGSAPGLDPIAEKPSEGTDTVSESSSTPQMVVWGTNVVVHQCKTKFKVFLMRYIDPDVERDEISENIDINQPIYMQKLEEIHTLEEPYLNVNCAHLKTFDEALYRQLICYPQEVIPAFDMAVNEMFFERYPAAVLEHQIQVRPFNADKTRNMRSLNPEDMDQLLSISGMVIRTSGVIPEMREAFFKCIICAFCTAVEVDRGRIAQPTLCTNCNTNHCFRLVHNRSEFTDKQLIKLQESPDDMAAGQTPHNVLLYAHNDLVDKVQPGDRVTVTGIYRAVPLKDKGRNIKSVYKTHVDVVHYRKVDSNRLYEEEEGKMHIFTPERVELLHLLSQKPDIYDRLSRAIAPSIYENDDIKKGILLQLFGGTKKKHSTLGRQNFRAEIHLLLCGDPGTSKSQLLQYVFNLVPRSQYTSGRGSSAVGLTAYVTKDPETRQLVLQTGALVLADNGVCCIDEFDKMNDSTRSVLHEVMEQQTLSIAKAGIICQLNARTSILAAANPAESQWNKKKNIIDNVQLPHTLLSRFDLIFLVLDPQDEQFDRRLANHLVSLYYVTRHEEEDTMFDVSVLRDYIAYAREHCSPTLSEEAQQRLIQAYVDMRKVGARRGQISAYPRQLESLIRLSEAHAKVRLSNVVTVEDVEEAWRLHREALKQSATDPLSGKIDVGILTTGLSTAARKKRADLVLAIKENLKKKGKVPTVPYQKLFKEVKDASQILITREQFEDALKEIQDEGAIVVMGKNTIRIC

Target ORF information:

RefSeq Version	XM_011208986.2
Organism	Bactrocera dorsalis(oriental fruit fly)
Definition	Bactrocera dorsalis DNA replication licensing factor MCM4 (LOC105228957), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011208986.2

ORF Insert Sequence:

ATGTCAAGTC CCGCTCGCAC ACCAGAAGGT GGGGTAACCC CAAGAGGTCA AGGCGAACGT 
ACTCCAACGC GAAACCCTGT AGTTCAGGAT GCAACAGATA CACCGATGCG TATGGGTCCA 
ACAACTCAAC GTCAGAGCCA ACAGCCATCG GAAATTAGTT TGCCTCCAAC ATCACCGGGC 
GGTTTGAGTT TACCAGCAAC AAGTCCAGCC CGTGCGCTGG GCATGAGTGA AATTGATCTA 
AGTTCCCCAT TGAACTATGG TACACCCAGC TCAATTGGTT CTATTCGTAC ACCACGTTCG 
GGTATTCGTG GTACACCTTT GCGAGCACGT CCCGATATAC GCACAGATAA AAGGATGCGT 
CAAGTGACAA TTGGTGGTTC TGCACCCGGT TTGGATCCAA TTGCTGAGAA GCCCTCAGAA 
GGAACTGATA CTGTGTCTGA GTCGTCATCA ACACCGCAGA TGGTTGTTTG GGGCACCAAT 
GTTGTGGTGC ATCAATGTAA AACAAAATTC AAAGTTTTTC TTATGCGCTA CATTGATCCT 
GACGTAGAGC GCGACGAAAT TTCTGAAAAT ATAGATATAA ATCAGCCAAT ATATATGCAA 
AAGTTGGAAG AGATTCACAC TCTAGAAGAG CCTTACCTAA ATGTGAACTG CGCACATTTG 
AAAACATTTG ATGAAGCACT CTATCGTCAG TTGATTTGTT ACCCTCAAGA AGTGATCCCA 
GCTTTCGATA TGGCAGTAAA TGAAATGTTT TTCGAACGAT ACCCAGCAGC TGTATTAGAG 
CATCAGATTC AAGTACGTCC CTTTAATGCG GATAAGACAC GAAACATGCG TTCACTCAAC 
CCTGAGGATA TGGATCAGTT GTTGAGTATT AGTGGAATGG TAATACGAAC ATCTGGTGTT 
ATTCCGGAAA TGCGTGAAGC TTTCTTCAAA TGTATAATAT GTGCTTTCTG CACCGCAGTT 
GAAGTCGACA GAGGACGCAT TGCTCAACCA ACCCTCTGTA CAAATTGTAA TACAAATCAT 
TGTTTCCGAT TGGTGCACAA TCGTTCAGAA TTTACAGATA AACAACTAAT TAAACTGCAA 
GAGTCGCCGG ACGACATGGC TGCCGGCCAA ACACCACACA ATGTGCTTCT ATATGCTCAC 
AATGATTTAG TGGATAAGGT TCAGCCTGGT GATCGTGTCA CCGTAACTGG TATATATCGC 
GCTGTCCCAT TGAAGGATAA GGGGCGGAAT ATTAAGAGCG TTTACAAAAC ACATGTTGAC 
GTAGTGCATT ATCGGAAAGT TGACAGCAAC CGTTTGTACG AAGAGGAAGA AGGAAAAATG 
CATATTTTTA CACCAGAGCG TGTGGAATTG TTACATTTAC TCTCACAGAA ACCAGATATA 
TACGATCGTT TATCCAGAGC CATTGCTCCT TCCATTTATG AAAATGATGA CATCAAGAAA 
GGTATTTTAT TACAATTGTT TGGTGGTACA AAGAAAAAAC ATTCAACTTT GGGCAGACAG 
AATTTCAGAG CGGAAATTCA CTTGCTTTTG TGTGGTGACC CCGGTACATC AAAGTCTCAG 
TTGCTACAAT ATGTTTTCAA TTTAGTACCG CGCTCACAGT ACACTTCGGG TAGAGGATCT 
TCGGCAGTTG GTTTAACTGC TTATGTTACA AAAGATCCGG AAACGCGACA ACTAGTGTTG 
CAAACCGGTG CACTGGTATT GGCTGATAAT GGTGTTTGCT GCATCGATGA ATTTGATAAA 
ATGAATGATT CCACGCGCAG TGTTCTGCAT GAAGTCATGG AACAGCAGAC CCTAAGTATT 
GCCAAGGCTG GTATCATTTG TCAGCTTAAC GCACGTACCT CTATTTTGGC TGCTGCTAAT 
CCAGCTGAAT CGCAGTGGAA TAAGAAAAAG AATATAATCG ACAATGTACA ATTGCCACAT 
ACACTCCTGT CAAGATTTGA TTTAATTTTC CTTGTACTGG ATCCACAAGA TGAGCAATTT 
GACCGCCGTT TGGCAAATCA TCTTGTATCG CTTTATTATG TGACTCGTCA CGAAGAAGAA 
GATACCATGT TTGACGTTAG TGTACTACGT GACTACATTG CTTATGCTCG TGAACACTGC 
TCTCCTACTT TATCGGAGGA AGCTCAACAA CGCCTCATTC AAGCATATGT TGATATGCGC 
AAAGTTGGTG CACGTCGCGG TCAAATATCA GCGTATCCCA GACAGCTGGA AAGTTTGATA 
CGTTTATCGG AAGCACATGC TAAAGTTCGC TTGAGTAATG TAGTAACTGT AGAGGATGTA 
GAAGAAGCTT GGAGATTACA CCGTGAAGCT TTGAAACAAT CTGCCACCGA TCCACTTTCT 
GGTAAGATAG ATGTGGGTAT TCTTACAACA GGTTTGTCGA CTGCTGCACG TAAGAAACGA 
GCTGATCTCG TATTAGCTAT TAAGGAAAAC CTTAAAAAGA AAGGCAAAGT GCCAACTGTT 
CCGTATCAGA AACTATTTAA AGAAGTCAAA GATGCCTCAC AAATTCTTAT TACACGCGAA 
CAATTCGAGG ATGCGCTCAA AGAAATTCAA GATGAAGGCG CCATTGTTGT GATGGGTAAA 
AATACCATCA GAATCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105228957 ( XM_011208986.3 ) cDNA ORF clone, Bactrocera dorsalis(oriental fruit fly) -> XP_011207288.1 Bactrocera dorsalis DNA replication licensing factor MCM4 (LOC105228957), mRNA.

Price & Availability↑

CloneID	OBa129677
Clone ID Related Accession (Same CDS sequence)	XM_011208986.2 , XM_011208986.3
Accession Version	XM_011208986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Bactrocera dorsalis(oriental fruit fly)
Product	DNA replication licensing factor MCM4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011876376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2019 this sequence version replaced XM_011208986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bactrocera dorsalis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGTC CCGCTCGCAC ACCAGAAGGT GGGGTAACCC CAAGAGGTCA AGGCGAACGT 
ACTCCAACGC GAAACCCTGT AGTTCAGGAT GCAACAGATA CACCGATGCG TATGGGTCCA 
ACAACTCAAC GTCAGAGCCA ACAGCCATCG GAAATTAGTT TGCCTCCAAC ATCACCGGGC 
GGTTTGAGTT TACCAGCAAC AAGTCCAGCC CGTGCGCTGG GCATGAGTGA AATTGATCTA 
AGTTCCCCAT TGAACTATGG TACACCCAGC TCAATTGGTT CTATTCGTAC ACCACGTTCG 
GGTATTCGTG GTACACCTTT GCGAGCACGT CCCGATATAC GCACAGATAA AAGGATGCGT 
CAAGTGACAA TTGGTGGTTC TGCACCCGGT TTGGATCCAA TTGCTGAGAA GCCCTCAGAA 
GGAACTGATA CTGTGTCTGA GTCGTCATCA ACACCGCAGA TGGTTGTTTG GGGCACCAAT 
GTTGTGGTGC ATCAATGTAA AACAAAATTC AAAGTTTTTC TTATGCGCTA CATTGATCCT 
GACGTAGAGC GCGACGAAAT TTCTGAAAAT ATAGATATAA ATCAGCCAAT ATATATGCAA 
AAGTTGGAAG AGATTCACAC TCTAGAAGAG CCTTACCTAA ATGTGAACTG CGCACATTTG 
AAAACATTTG ATGAAGCACT CTATCGTCAG TTGATTTGTT ACCCTCAAGA AGTGATCCCA 
GCTTTCGATA TGGCAGTAAA TGAAATGTTT TTCGAACGAT ACCCAGCAGC TGTATTAGAG 
CATCAGATTC AAGTACGTCC CTTTAATGCG GATAAGACAC GAAACATGCG TTCACTCAAC 
CCTGAGGATA TGGATCAGTT GTTGAGTATT AGTGGAATGG TAATACGAAC ATCTGGTGTT 
ATTCCGGAAA TGCGTGAAGC TTTCTTCAAA TGTATAATAT GTGCTTTCTG CACCGCAGTT 
GAAGTCGACA GAGGACGCAT TGCTCAACCA ACCCTCTGTA CAAATTGTAA TACAAATCAT 
TGTTTCCGAT TGGTGCACAA TCGTTCAGAA TTTACAGATA AACAACTAAT TAAACTGCAA 
GAGTCGCCGG ACGACATGGC TGCCGGCCAA ACACCACACA ATGTGCTTCT ATATGCTCAC 
AATGATTTAG TGGATAAGGT TCAGCCTGGT GATCGTGTCA CCGTAACTGG TATATATCGC 
GCTGTCCCAT TGAAGGATAA GGGGCGGAAT ATTAAGAGCG TTTACAAAAC ACATGTTGAC 
GTAGTGCATT ATCGGAAAGT TGACAGCAAC CGTTTGTACG AAGAGGAAGA AGGAAAAATG 
CATATTTTTA CACCAGAGCG TGTGGAATTG TTACATTTAC TCTCACAGAA ACCAGATATA 
TACGATCGTT TATCCAGAGC CATTGCTCCT TCCATTTATG AAAATGATGA CATCAAGAAA 
GGTATTTTAT TACAATTGTT TGGTGGTACA AAGAAAAAAC ATTCAACTTT GGGCAGACAG 
AATTTCAGAG CGGAAATTCA CTTGCTTTTG TGTGGTGACC CCGGTACATC AAAGTCTCAG 
TTGCTACAAT ATGTTTTCAA TTTAGTACCG CGCTCACAGT ACACTTCGGG TAGAGGATCT 
TCGGCAGTTG GTTTAACTGC TTATGTTACA AAAGATCCGG AAACGCGACA ACTAGTGTTG 
CAAACCGGTG CACTGGTATT GGCTGATAAT GGTGTTTGCT GCATCGATGA ATTTGATAAA 
ATGAATGATT CCACGCGCAG TGTTCTGCAT GAAGTCATGG AACAGCAGAC CCTAAGTATT 
GCCAAGGCTG GTATCATTTG TCAGCTTAAC GCACGTACCT CTATTTTGGC TGCTGCTAAT 
CCAGCTGAAT CGCAGTGGAA TAAGAAAAAG AATATAATCG ACAATGTACA ATTGCCACAT 
ACACTCCTGT CAAGATTTGA TTTAATTTTC CTTGTACTGG ATCCACAAGA TGAGCAATTT 
GACCGCCGTT TGGCAAATCA TCTTGTATCG CTTTATTATG TGACTCGTCA CGAAGAAGAA 
GATACCATGT TTGACGTTAG TGTACTACGT GACTACATTG CTTATGCTCG TGAACACTGC 
TCTCCTACTT TATCGGAGGA AGCTCAACAA CGCCTCATTC AAGCATATGT TGATATGCGC 
AAAGTTGGTG CACGTCGCGG TCAAATATCA GCGTATCCCA GACAGCTGGA AAGTTTGATA 
CGTTTATCGG AAGCACATGC TAAAGTTCGC TTGAGTAATG TAGTAACTGT AGAGGATGTA 
GAAGAAGCTT GGAGATTACA CCGTGAAGCT TTGAAACAAT CTGCCACCGA TCCACTTTCT 
GGTAAGATAG ATGTGGGTAT TCTTACAACA GGTTTGTCGA CTGCTGCACG TAAGAAACGA 
GCTGATCTCG TATTAGCTAT TAAGGAAAAC CTTAAAAAGA AAGGCAAAGT GCCAACTGTT 
CCGTATCAGA AACTATTTAA AGAAGTCAAA GATGCCTCAC AAATTCTTAT TACACGCGAA 
CAATTCGAGG ATGCGCTCAA AGAAATTCAA GATGAAGGCG CCATTGTTGT GATGGGTAAA 
AATACCATCA GAATCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011207288.1
CDS	119..2719
Translation	MSSPARTPEGGVTPRGQGERTPTRNPVVQDATDTPMRMGPTTQRQSQQPSEISLPPTSPGGLSLPATSPARALGMSEIDLSSPLNYGTPSSIGSIRTPRSGIRGTPLRARPDIRTDKRMRQVTIGGSAPGLDPIAEKPSEGTDTVSESSSTPQMVVWGTNVVVHQCKTKFKVFLMRYIDPDVERDEISENIDINQPIYMQKLEEIHTLEEPYLNVNCAHLKTFDEALYRQLICYPQEVIPAFDMAVNEMFFERYPAAVLEHQIQVRPFNADKTRNMRSLNPEDMDQLLSISGMVIRTSGVIPEMREAFFKCIICAFCTAVEVDRGRIAQPTLCTNCNTNHCFRLVHNRSEFTDKQLIKLQESPDDMAAGQTPHNVLLYAHNDLVDKVQPGDRVTVTGIYRAVPLKDKGRNIKSVYKTHVDVVHYRKVDSNRLYEEEEGKMHIFTPERVELLHLLSQKPDIYDRLSRAIAPSIYENDDIKKGILLQLFGGTKKKHSTLGRQNFRAEIHLLLCGDPGTSKSQLLQYVFNLVPRSQYTSGRGSSAVGLTAYVTKDPETRQLVLQTGALVLADNGVCCIDEFDKMNDSTRSVLHEVMEQQTLSIAKAGIICQLNARTSILAAANPAESQWNKKKNIIDNVQLPHTLLSRFDLIFLVLDPQDEQFDRRLANHLVSLYYVTRHEEEDTMFDVSVLRDYIAYAREHCSPTLSEEAQQRLIQAYVDMRKVGARRGQISAYPRQLESLIRLSEAHAKVRLSNVVTVEDVEEAWRLHREALKQSATDPLSGKIDVGILTTGLSTAARKKRADLVLAIKENLKKKGKVPTVPYQKLFKEVKDASQILITREQFEDALKEIQDEGAIVVMGKNTIRIC

Target ORF information:

RefSeq Version	XM_011208986.3
Organism	Bactrocera dorsalis(oriental fruit fly)
Definition	Bactrocera dorsalis DNA replication licensing factor MCM4 (LOC105228957), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011208986.3

ORF Insert Sequence:

ATGTCAAGTC CCGCTCGCAC ACCAGAAGGT GGGGTAACCC CAAGAGGTCA AGGCGAACGT 
ACTCCAACGC GAAACCCTGT AGTTCAGGAT GCAACAGATA CACCGATGCG TATGGGTCCA 
ACAACTCAAC GTCAGAGCCA ACAGCCATCG GAAATTAGTT TGCCTCCAAC ATCACCGGGC 
GGTTTGAGTT TACCAGCAAC AAGTCCAGCC CGTGCGCTGG GCATGAGTGA AATTGATCTA 
AGTTCCCCAT TGAACTATGG TACACCCAGC TCAATTGGTT CTATTCGTAC ACCACGTTCG 
GGTATTCGTG GTACACCTTT GCGAGCACGT CCCGATATAC GCACAGATAA AAGGATGCGT 
CAAGTGACAA TTGGTGGTTC TGCACCCGGT TTGGATCCAA TTGCTGAGAA GCCCTCAGAA 
GGAACTGATA CTGTGTCTGA GTCGTCATCA ACACCGCAGA TGGTTGTTTG GGGCACCAAT 
GTTGTGGTGC ATCAATGTAA AACAAAATTC AAAGTTTTTC TTATGCGCTA CATTGATCCT 
GACGTAGAGC GCGACGAAAT TTCTGAAAAT ATAGATATAA ATCAGCCAAT ATATATGCAA 
AAGTTGGAAG AGATTCACAC TCTAGAAGAG CCTTACCTAA ATGTGAACTG CGCACATTTG 
AAAACATTTG ATGAAGCACT CTATCGTCAG TTGATTTGTT ACCCTCAAGA AGTGATCCCA 
GCTTTCGATA TGGCAGTAAA TGAAATGTTT TTCGAACGAT ACCCAGCAGC TGTATTAGAG 
CATCAGATTC AAGTACGTCC CTTTAATGCG GATAAGACAC GAAACATGCG TTCACTCAAC 
CCTGAGGATA TGGATCAGTT GTTGAGTATT AGTGGAATGG TAATACGAAC ATCTGGTGTT 
ATTCCGGAAA TGCGTGAAGC TTTCTTCAAA TGTATAATAT GTGCTTTCTG CACCGCAGTT 
GAAGTCGACA GAGGACGCAT TGCTCAACCA ACCCTCTGTA CAAATTGTAA TACAAATCAT 
TGTTTCCGAT TGGTGCACAA TCGTTCAGAA TTTACAGATA AACAACTAAT TAAACTGCAA 
GAGTCGCCGG ACGACATGGC TGCCGGCCAA ACACCACACA ATGTGCTTCT ATATGCTCAC 
AATGATTTAG TGGATAAGGT TCAGCCTGGT GATCGTGTCA CCGTAACTGG TATATATCGC 
GCTGTCCCAT TGAAGGATAA GGGGCGGAAT ATTAAGAGCG TTTACAAAAC ACATGTTGAC 
GTAGTGCATT ATCGGAAAGT TGACAGCAAC CGTTTGTACG AAGAGGAAGA AGGAAAAATG 
CATATTTTTA CACCAGAGCG TGTGGAATTG TTACATTTAC TCTCACAGAA ACCAGATATA 
TACGATCGTT TATCCAGAGC CATTGCTCCT TCCATTTATG AAAATGATGA CATCAAGAAA 
GGTATTTTAT TACAATTGTT TGGTGGTACA AAGAAAAAAC ATTCAACTTT GGGCAGACAG 
AATTTCAGAG CGGAAATTCA CTTGCTTTTG TGTGGTGACC CCGGTACATC AAAGTCTCAG 
TTGCTACAAT ATGTTTTCAA TTTAGTACCG CGCTCACAGT ACACTTCGGG TAGAGGATCT 
TCGGCAGTTG GTTTAACTGC TTATGTTACA AAAGATCCGG AAACGCGACA ACTAGTGTTG 
CAAACCGGTG CACTGGTATT GGCTGATAAT GGTGTTTGCT GCATCGATGA ATTTGATAAA 
ATGAATGATT CCACGCGCAG TGTTCTGCAT GAAGTCATGG AACAGCAGAC CCTAAGTATT 
GCCAAGGCTG GTATCATTTG TCAGCTTAAC GCACGTACCT CTATTTTGGC TGCTGCTAAT 
CCAGCTGAAT CGCAGTGGAA TAAGAAAAAG AATATAATCG ACAATGTACA ATTGCCACAT 
ACACTCCTGT CAAGATTTGA TTTAATTTTC CTTGTACTGG ATCCACAAGA TGAGCAATTT 
GACCGCCGTT TGGCAAATCA TCTTGTATCG CTTTATTATG TGACTCGTCA CGAAGAAGAA 
GATACCATGT TTGACGTTAG TGTACTACGT GACTACATTG CTTATGCTCG TGAACACTGC 
TCTCCTACTT TATCGGAGGA AGCTCAACAA CGCCTCATTC AAGCATATGT TGATATGCGC 
AAAGTTGGTG CACGTCGCGG TCAAATATCA GCGTATCCCA GACAGCTGGA AAGTTTGATA 
CGTTTATCGG AAGCACATGC TAAAGTTCGC TTGAGTAATG TAGTAACTGT AGAGGATGTA 
GAAGAAGCTT GGAGATTACA CCGTGAAGCT TTGAAACAAT CTGCCACCGA TCCACTTTCT 
GGTAAGATAG ATGTGGGTAT TCTTACAACA GGTTTGTCGA CTGCTGCACG TAAGAAACGA 
GCTGATCTCG TATTAGCTAT TAAGGAAAAC CTTAAAAAGA AAGGCAAAGT GCCAACTGTT 
CCGTATCAGA AACTATTTAA AGAAGTCAAA GATGCCTCAC AAATTCTTAT TACACGCGAA 
CAATTCGAGG ATGCGCTCAA AGAAATTCAA GATGAAGGCG CCATTGTTGT GATGGGTAAA 
AATACCATCA GAATCTGTTA A