BO83DRAFT_379919 cDNA ORF clone,

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gRNAs

The following BO83DRAFT_379919 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BO83DRAFT_379919 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs206151	XM_025531615.1 Latest version!	Aspergillus eucalypticola CBS 122712 histidine-tagged glucoamylase (BO83DRAFT_379919), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BO83DRAFT_379919 ( XM_025531615.1 ) cDNA ORF clone, -> XP_025386521.1 Aspergillus eucalypticola CBS 122712 histidine-tagged glucoamylase (BO83DRAFT_379919), mRNA.

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CloneID	OAs206151
Clone ID Related Accession (Same CDS sequence)	XM_025531615.1
Accession Version	XM_025531615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-05
Organism	Aspergillus eucalypticola CBS 122712
Product	histidine-tagged glucoamylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_020290292). ##Metadata-START## Organism Display Name :: Aspergillus eucalypticola CBS 122712 v1.0 GOLD Stamp ID :: Gp0047226 ##Metadata-END##

ATGTCGTTCC GATCTCTTCT CGCCCTGAGC GGCCTTGTCT GCTCGGGGTT GGCAAGTGTG 
ATTTCCAAGC GCGCGACCTT GGATTCGTGG TTGAGCAACG AAGCGACCGT GGCTCGTACT 
GCGATCCTGA ATAACATTGG GGCGGACGGT GCTTGGGTGT CGGGCGCGGA CTCTGGCATA 
GTCGTTGCCA GTCCCAGCAC CGATAACCCG GACTACTTCT ACACCTGGAC TCGCGACTCT 
GGTCTCGTCA TCAAGACCCT CGTCGATCTC TTCCGCAATG GAGATACTGA TCTCCTTTCC 
ACCATTGAGA ACTACATCTC CTCTCAGGCA ATTGTTCAGG GTATCAGTAA CCCCTCTGGT 
GATCTGTCCA GCGGTGGTCT TGGTGAGCCC AAGTTCAATG TCGATGAGAC TGCCTACACC 
GGGTCTTGGG GACGGCCGCA GCGTGATGGT CCTGCCCTGA GAGCAACTGC TATGATCGGC 
TTCGGCCAGT GGCTGCTTGA CAATGGCTAC ACCAGCGCTG CAACGGAGAT TGTTTGGCCC 
CTCGTTAGGA ACGACCTGTC GTATGTGGCT CAGTACTGGA ACCAGACGGG ATATGATCTC 
TGGGAAGAAG TTAATGGCTC GTCCTTCTTC ACTATTGCCG TGCAACACCG TGCCCTCGTC 
GAAGGTAGTG CCTTCGCGAC GGCCGTCGGC TCGTCCTGCT CCTGGTGTGA TTCGCAGGCA 
CCTCAGATTC TCTGCTACCT GCAGTCCTTC TGGACCGGCA GCTATATCCT GGCCAACTTT 
GACAGCAGCC GTTCCGGCAA GGACACAAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAT 
CCTGAGGCTG GATGCGACGA CTCCACCTTT CAGCCCTGCT CCCCGCGTGC GCTCGCCAAC 
CATAAGGAGG TTGTAGACTC TTTCCGCTCG ATCTATACTC TCAACGATGG TCTCAGTGAC 
AGTGAGGCGG TTGCCGTCGG TCGGTACCCT GAGGATAGCT ACTACAACGG CAACCCGTGG 
TTCCTGTGCA CCTTGGCTGC CGCGGAACAG CTGTACGATG CTCTGTACCA GTGGGACAAG 
CAGGGGTCGT TGGAGATCAC AGACGTGTCA CTAGACTTCT TCCAGGCTCT GTACAGTGAT 
GCTGCCACCG GCACGTACTC TTCGTCCAGC TCGACCTATA GCAGCATTGT GGATGCCGTC 
AAGACTTTCG CTGATGGTTT CGTTTCTATT GTGGAAACTC ACGCCGCAAG CAACGGCTCT 
CTGTCTGAGC AATTCGACAA GTCTGATGGC GACGAGCTTT CTGCTCGTGA TCTGACCTGG 
TCTTACGCTG CTCTGCTGAC CGCCAACAAC CGTCGGAACT CTGTCGTGCC CCCGTCTTGG 
GGTGAGACCT CTGCCAGCAG CGTGCCCGGC ACCTGTGCGG CTACCTCTGC CTCTGGTACC 
TACAGCAGTG TGACCGTCAC CTCGTGGCCG AGCATCGTGG CTACTGGTGG CACCACTACG 
ACGGCTACCA CCACTGGATC CGGCGGCGTG ACCTCGACCA GCAAGACCAC CACAACTGCT 
AGTAAGACCA GCACCACTAC GTCCTCGACC TCCTGCACCA CTCCCACTGC CGTAGCTGTG 
ACCTTTGATC TGACGGCGAC CACCACCTAC GGCGAGAACA TCTACCTGGT CGGGTCGATC 
TCTCAGCTCG GTGACTGGGA CACCAGCGAC GGCATAGCTC TGAGCGCTGA CAAGTACACT 
TCCAGCAACC CGCTATGGTA TGTAACTGTG ACTCTGCCGG CTGGTGAGTC ATTTGAGTAC 
AAGTTCATCC GCGTTGAGAG CGATGACTCC GTGGAGTGGG AGAGTGACCC GAACCGGGAA 
TACACCGTTC CTCAGGCATG CGGCGAGTCG ACCGCGACGG TGACTGACAC CTGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025386521.1
CDS	253..2172
Translation	MSFRSLLALSGLVCSGLASVISKRATLDSWLSNEATVARTAILNNIGADGAWVSGADSGIVVASPSTDNPDYFYTWTRDSGLVIKTLVDLFRNGDTDLLSTIENYISSQAIVQGISNPSGDLSSGGLGEPKFNVDETAYTGSWGRPQRDGPALRATAMIGFGQWLLDNGYTSAATEIVWPLVRNDLSYVAQYWNQTGYDLWEEVNGSSFFTIAVQHRALVEGSAFATAVGSSCSWCDSQAPQILCYLQSFWTGSYILANFDSSRSGKDTNTLLGSIHTFDPEAGCDDSTFQPCSPRALANHKEVVDSFRSIYTLNDGLSDSEAVAVGRYPEDSYYNGNPWFLCTLAAAEQLYDALYQWDKQGSLEITDVSLDFFQALYSDAATGTYSSSSSTYSSIVDAVKTFADGFVSIVETHAASNGSLSEQFDKSDGDELSARDLTWSYAALLTANNRRNSVVPPSWGETSASSVPGTCAATSASGTYSSVTVTSWPSIVATGGTTTTATTTGSGGVTSTSKTTTTASKTSTTTSSTSCTTPTAVAVTFDLTATTTYGENIYLVGSISQLGDWDTSDGIALSADKYTSSNPLWYVTVTLPAGESFEYKFIRVESDDSVEWESDPNREYTVPQACGESTATVTDTWR

Target ORF information:

RefSeq Version	XM_025531615.1
Organism	Aspergillus eucalypticola CBS 122712
Definition	Aspergillus eucalypticola CBS 122712 histidine-tagged glucoamylase (BO83DRAFT_379919), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025531615.1

ORF Insert Sequence:

ATGTCGTTCC GATCTCTTCT CGCCCTGAGC GGCCTTGTCT GCTCGGGGTT GGCAAGTGTG 
ATTTCCAAGC GCGCGACCTT GGATTCGTGG TTGAGCAACG AAGCGACCGT GGCTCGTACT 
GCGATCCTGA ATAACATTGG GGCGGACGGT GCTTGGGTGT CGGGCGCGGA CTCTGGCATA 
GTCGTTGCCA GTCCCAGCAC CGATAACCCG GACTACTTCT ACACCTGGAC TCGCGACTCT 
GGTCTCGTCA TCAAGACCCT CGTCGATCTC TTCCGCAATG GAGATACTGA TCTCCTTTCC 
ACCATTGAGA ACTACATCTC CTCTCAGGCA ATTGTTCAGG GTATCAGTAA CCCCTCTGGT 
GATCTGTCCA GCGGTGGTCT TGGTGAGCCC AAGTTCAATG TCGATGAGAC TGCCTACACC 
GGGTCTTGGG GACGGCCGCA GCGTGATGGT CCTGCCCTGA GAGCAACTGC TATGATCGGC 
TTCGGCCAGT GGCTGCTTGA CAATGGCTAC ACCAGCGCTG CAACGGAGAT TGTTTGGCCC 
CTCGTTAGGA ACGACCTGTC GTATGTGGCT CAGTACTGGA ACCAGACGGG ATATGATCTC 
TGGGAAGAAG TTAATGGCTC GTCCTTCTTC ACTATTGCCG TGCAACACCG TGCCCTCGTC 
GAAGGTAGTG CCTTCGCGAC GGCCGTCGGC TCGTCCTGCT CCTGGTGTGA TTCGCAGGCA 
CCTCAGATTC TCTGCTACCT GCAGTCCTTC TGGACCGGCA GCTATATCCT GGCCAACTTT 
GACAGCAGCC GTTCCGGCAA GGACACAAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAT 
CCTGAGGCTG GATGCGACGA CTCCACCTTT CAGCCCTGCT CCCCGCGTGC GCTCGCCAAC 
CATAAGGAGG TTGTAGACTC TTTCCGCTCG ATCTATACTC TCAACGATGG TCTCAGTGAC 
AGTGAGGCGG TTGCCGTCGG TCGGTACCCT GAGGATAGCT ACTACAACGG CAACCCGTGG 
TTCCTGTGCA CCTTGGCTGC CGCGGAACAG CTGTACGATG CTCTGTACCA GTGGGACAAG 
CAGGGGTCGT TGGAGATCAC AGACGTGTCA CTAGACTTCT TCCAGGCTCT GTACAGTGAT 
GCTGCCACCG GCACGTACTC TTCGTCCAGC TCGACCTATA GCAGCATTGT GGATGCCGTC 
AAGACTTTCG CTGATGGTTT CGTTTCTATT GTGGAAACTC ACGCCGCAAG CAACGGCTCT 
CTGTCTGAGC AATTCGACAA GTCTGATGGC GACGAGCTTT CTGCTCGTGA TCTGACCTGG 
TCTTACGCTG CTCTGCTGAC CGCCAACAAC CGTCGGAACT CTGTCGTGCC CCCGTCTTGG 
GGTGAGACCT CTGCCAGCAG CGTGCCCGGC ACCTGTGCGG CTACCTCTGC CTCTGGTACC 
TACAGCAGTG TGACCGTCAC CTCGTGGCCG AGCATCGTGG CTACTGGTGG CACCACTACG 
ACGGCTACCA CCACTGGATC CGGCGGCGTG ACCTCGACCA GCAAGACCAC CACAACTGCT 
AGTAAGACCA GCACCACTAC GTCCTCGACC TCCTGCACCA CTCCCACTGC CGTAGCTGTG 
ACCTTTGATC TGACGGCGAC CACCACCTAC GGCGAGAACA TCTACCTGGT CGGGTCGATC 
TCTCAGCTCG GTGACTGGGA CACCAGCGAC GGCATAGCTC TGAGCGCTGA CAAGTACACT 
TCCAGCAACC CGCTATGGTA TGTAACTGTG ACTCTGCCGG CTGGTGAGTC ATTTGAGTAC 
AAGTTCATCC GCGTTGAGAG CGATGACTCC GTGGAGTGGG AGAGTGACCC GAACCGGGAA 
TACACCGTTC CTCAGGCATG CGGCGAGTCG ACCGCGACGG TGACTGACAC CTGGCGGTAG