EFS cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFS
Entrez Gene ID	844066
Full Name	histone-lysine N-methyltransferase
Synonyms	ASH1 HOMOLOG 2,ASHH2,CAROTENOID CHLOROPLAST REGULATORY1,CCR1,EARLY FLOWERING IN SHORT DAYS,LAZ2,LAZARUS 2,SDG8,SET DOMAIN GROUP 8,T14N5.15,T14N5_15
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001084367.2	NP_001077836.1	histone-lysine N-methyltransferase
NM_106379.9	NP_177854.6	histone-lysine N-methyltransferase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	EFS	NP_177854.6
Oryza sativa (rice)	Os02g0554000	NP_001047114.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SDG8 and SDG25 contribute to plant immunity directly through HLM or indirectly through H2Bubn and by regulating expression of plant immunity genes, accumulation of lipids, biosynthesis of carotenoids, and maintenance of cuticle integrity.
Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases.

We show that the sdg8 mutation is epistatic to the sdg26 one, leading to a sdg8 sdg26 double mutant exhibiting defects similar to sdg8 (reduced level of H3K36me3, increased level of H3K36me1, reduced plant body size, early flowering
Title: Interplay of the histone methyltransferases SDG8 and SDG26 in the regulation of transcription and plant flowering and development.

review of role in gene activation
Title: ATX1/AtCOMPASS and the H3K4me3 marks: how do they activate Arabidopsis genes?

SDG8 plays an essential role in mediating BR-regulated gene expression
Title: Histone lysine methyltransferase SDG8 is involved in brassinosteroid-regulated gene expression in Arabidopsis thaliana.

EFS is necessary for high-level expression of PIF1 mRNA in imbibed seeds.
Title: A histone methyltransferase inhibits seed germination by increasing PIF1?mRNA expression in imbibed seeds.

The following EFS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb28208	NM_106379.9 Latest version!	Arabidopsis thaliana histone-lysine N-methyltransferase (EFS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb28209	NM_001084367.2 Latest version!	Arabidopsis thaliana histone-lysine N-methyltransferase (EFS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFS ( NM_106379.9 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177854.6 Arabidopsis thaliana histone-lysine N-methyltransferase (EFS), mRNA.

Price & Availability↑

CloneID	OAb28208
Clone ID Related Accession (Same CDS sequence)	NM_106379.9
Accession Version	NM_106379.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	histone-lysine N-methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106379.8.

ATGGACTGTA AGGAAAACGG TGTTGGTGAC GCTTCTGGGT GCAATATTGA TGCTAACTCT 
CTGGCCTCAA ATCTTGCGAT GAATACCAAT GAAGATTTCT ATGAGAAATT GAGTTCTCGT 
GGGCAAAATT TAGATTCAGT CAGTAGTTTG GAGATTCCTC AGACTGCTTC ATCTGTAAAC 
CATACGATTG AAGGCCAAAG GAAGTGCTTT ACAGAGATTG AGCAGATGGG ATACGGGAAC 
AGTAATAGTC AAGAAGATGC TGGAAACACT GATGATGATC TATATGTTTG TTACAATGCT 
GATGACACTC AGGAGCAGGG AGTGGTTTCA GGTGAGCTTG AACAAAGTCA AGAATTAATT 
TGTGATACTG ATTTACTGGT AAATTGCAAC AAGCTGGATG ATGGAAAGGA AAGTCAGGAC 
ACAAATGTGT CCCTTGTATC CATTTTTTCT GGGAGTATGC AAGAGAAAGA AGCTCCTCAG 
GCTAAAGAAG ATGAAGGTTA TGGTGGCACA ACATTGCCTA TAGGGGGCAG TGGAATAGAT 
ACGGAATCAA CTTTTGTGAA TGATGCACCA GAACAATTCG AGTCTTTGGA AACCACAAAG 
CACATAAAAC CTGATGAAGT TGAAAGTGAT GGTATTAGTT ATAGGTTTGA TGATGGAGGT 
AAGGAAGGAA GAAATGGACC ATCGAGTGAT CTAGATACTG GTTCTTCTGA TGATATATCT 
CTCAGTCAGA GTTTTTCGTT TCCAGATTCG TTATTGGACT CTAGTGTATT TGGCTGTAGC 
GCCACAGAAA GTTATCTGGA GGATGCAATT GATATTGAAG GCAATGGGAC TATTGTTGTA 
TCCCCTTCAT TAGCTATCAC AGAAATGTTA AATAATGATG ATGGTGGCCT ATGTTCACAT 
GATCTTAACA AGATCACAGT CACAGAGACG ATCAATCCAG ATTTGAAGTT GGTACGTGAG 
GATAGGCTTG ACACTGATCT TTCCGTGATG AATGAAAAGA TGCTAAAAAA CCATGTTGGG 
GATTCATCAA GTGAAAGTGC CGTGGCCGCT TTGAGTATGA ATAATGGTAT GGCTGCTGAT 
CTGAGAGCTG AAAATTTCAG TCAGAGTTCA CCCATAGACG AAAAAACTTT AGATATGGAA 
GCCAATTCCC CTATTACTGA CTCTTCTTTG ATATGGAATT TTCCATTGAA CTTTGGAAGT 
GGAGGAATCG AAGTCTGTAA TCCTGAAAAT GCAGTTGAGC CACTCAGAAT AGTAGATGAC 
AATGGCAGAA TAGGTGGTGA AGTTGCTTCT GCATCAGGGA GTGATTTTTG TGAAGCTGGC 
ATGTCTTCTT CCCGAAGGAA GGCCCGAGAC GGGAAACAAT GTAAAGTGGT GCAGACAAAA 
ACGAGTGCTC GCCATCTGAG AAAATCCTCT AGAAAGAAAC AATCAGAAAG AGACATTGAG 
TCAATTTTCA AGTGCTCAAA GCAGAAGAGG AGCTCTCTTT TAAAAACAAG TCGTTCATCT 
GAGTGGGGAT TACCAAGCAA GACCACTGAA ATCTTCTTAC AGAGCAATAA TATTCCCTAT 
GATGGACCTC CGCATCATGA ACCACAGAGA TCTCAGGGAA ATCTTAATAA TGGAGAGCAT 
AATAGAAGTT CTCATAACGG GAATGTAGAA GGATCTAACA GAAACATCCA AGCATCAAGT 
GGCTCTTGCC TTCGTTTGAA AGTTAAATTT GGTAAATCAG GTGGCCAAAA TCCTCTGAAC 
ATTACAGTCT CTAAGGTCAG TGGTAACTCC TTACCTGGTA ATGGTATTGT AAAAGCAGGA 
ACATGTTTAG AATTGCCAGG ATCAGCACAT TTTGGTGAGG ATAAAATGCA AACTGTGGAA 
ACTAAAGAGG ACTTGGTAGA GAAAAGCAAC CCTGTGGAGA AAGTTTCATA TCTTCAGTCA 
TCTGATTCTA TGAGGGATAA GAAATATAAC CAAGACGCTG GGGGTTTATG TAGGAAGGTG 
GGTGGTGATG TTTTAGATGA TGACCCACAC CTTTCCTCTA TTAGAATGGT TGAGGAGTGC 
GAGAGGGCCA CTGGAACGCA GTCCCTGGAC GCTGAAACTT CACCAGATTC TGAAGTTATC 
AACTCTGTGC CTGATTCCAT TGTCAATATT GAACATAAAG AGGGTCTGCA TCATGGTTTT 
TTTAGCACTC CAGAAGATGT GGTCAAGAAG AACAGAGTAT TAGAAAAAGA AGATGAATTG 
CGTGCTTCAA AATCTCCTTC AGAAAATGGT TCACATCTAA TTCCCAATGC AAAAAAAGCT 
AAACATCCGA AGTCTAAAAG TAATGGAACA AAGAAAGGTA AATCCAAGTT TTCCGAATCT 
GCCAAAGACG GGAGAAAAAA TGAATCACAT GAAGGGGTAG AGCAACGCAA ATCCCTGAAT 
ACCAGTATGG GAAGGGATGA TAGTGATTAT CCTGAAGTAG GGAGAATAGA GTCTCACAAA 
ACAACAGGTG CCCTTCTAGA TGCTGATATT GGGAAAACCA GTGCCACTTA TGGTACCATA 
TCATCAGATG TGACCCATGG GGAAATGGTT GTGGATGTTA CTATTGAAGA TAGCTATTCC 
ACAGAGAGTG CCTGGGTTCG ATGTGATGAT TGCTTTAAAT GGCGACGAAT ACCTGCTTCT 
GTTGTAGGAT CAATTGACGA GAGCTCTAGA TGGATCTGTA TGAACAACTC AGATAAAAGA 
TTTGCGGATT GCTCAAAATC TCAAGAGATG TCAAATGAAG AAATTAATGA AGAGTTGGGC 
ATAGGACAGG ATGAAGCAGA TGCATATGAT TGTGATGCGG CTAAAAGAGG GAAAGAAAAG 
GAACAGAAGA GCAAGCGTTT GACAGGTAAG CAAAAGGCGT GCTTCAAGGC TATAAAAACA 
AACCAGTTCC TTCATCGCAA TCGTAAATCT CAAACAATTG ACGAGATAAT GGTTTGTCAC 
TGCAAACCAT CACCTGATGG TAGGTTGGGT TGTGGAGAAG AATGCCTCAA TAGAATGCTT 
AACATTGAAT GTCTTCAAGG TACCTGTCCA GCTGGCGATT TGTGTTCAAA TCAGCAGTTT 
CAAAAACGGA AGTATGTTAA GTTTGAGAGA TTCCAATCCG GTAAGAAGGG TTATGGCCTG 
AGATTGCTCG AGGATGTACG AGAGGGGCAA TTCCTAATTG AATATGTTGG AGAGGTGCTT 
GATATGCAAT CCTATGAGAC TCGCCAAAAA GAATATGCTT TCAAGGGTCA GAAACATTTC 
TATTTCATGA CACTAAATGG GAATGAGGTA ATAGATGCTG GTGCAAAGGG AAACCTAGGG 
CGTTTCATTA ACCATAGCTG TGAACCAAAC TGCCGTACTG AAAAGTGGAT GGTGAATGGT 
GAAATTTGCG TTGGAATATT CTCCATGCAA GACCTTAAGA AGGGTCAAGA GTTGACATTT 
GACTACAACT ATGTGAGGGT TTTTGGTGCT GCCGCCAAAA AGTGCTATTG TGGATCATCA 
CATTGCAGAG GGTATATTGG GGGAGATCCT CTGAATGGTG ATGTAATTAT TCAAAGTGAT 
TCAGATGAAG AGTATCCTGA ACTTGTGATC CTTGATGATG ATGAAAGTGG AGAAGGAATC 
TTAGGTGCAA CATCTAGGAC CTTCACTGAT GACGCTGACG AGCAAATGCC ACAGAGCTTT 
GAAAAGGTTA ATGGTTACAA GGACCTTGCT CCTGATAATA CCCAAACACA GAGCTCAGTA 
TCTGTAAAAC TTCCAGAGAG AGAAATTCCT CCACCTCTTC TTCAGCCAAC TGAAGTTTTG 
AAGGAACTTT CGTCAGGCAT ATCTATTACT GCTGTCCAGC AAGAGGTTCC CGCTGAAAAG 
AAGACTAAAA GCACATCTCC CACGTCCAGT TCTCTTAGCA GAATGTCCCC GGGTGGTACA 
AATTCTGATA AGACGACAAA GCATGGATCA GGAGAAGATA AAAAGATACT TCCACGTCCT 
CGTCCTCGTA TGAAAACTTC TCGCTCATCT GAGTCTAGTA AGCGAGACAA AGGAGGTATT 
TATCCTGGTG TTAACAAAGC ACAGGTTATA CCAGTTAATA AGTTGCAACA GCAGCCCATC 
AAATCTAAAG GATCAGAGAA AGTTTCTCCC AGTATCGAAA CATTTGAAGG GAAACTGAAC 
GAGTTACTAG ATGCCGTAGG AGGGATAAGC AAGCGGAGGG ATTCAGCAAA AGGCTACTTG 
AAACTTCTGC TGCTCACTGC CGCTTCGCGA GGCACGGATG AAGAAGGAAT TTATAGCAAT 
CGAGATCTTT CAATGATTCT TGATGCCCTT TTGAAGACAA AGTCAAAATC TGTTTTAGTG 
GACATAATCA ACAAGAATGG TCTGCAAATG TTACATAATA TCATGAAACA ATACCGGGGT 
GATTTTAAAA GGATCCCTAT AATCCGGAAA CTTCTGAAGG TATTAGAGTA TCTTGCCACA 
AGGAAAATCC TTGCACTGGA GCATATAATC AGAAGGCCTC CTTTTGCAGG GATGGAAAGC 
TTTAAGGACT CTGTTCTATC ATTCACCGAG CATGACGACT ATACGGTTCA TAATATTGCG 
CGGAGCTTCC GAGACAGATG GATACCTAAG CATTTTAGAA AACCTTGGCG CATCAACAGG 
GAGGAGAGAT CGGAGTCTAT GAGGTCACCT ATAAACAGAA GGTTCAGAGC ATCACAAGAA 
CCTCGATATG ATCATCAGTC CCCAAGACCT GCAGAACCAG CTGCGTCTGT CACATCATCA 
AAGGCTGCAA CTCCTGAAAC AGCATCTGTA TCTGAGGGAT ATTCAGAACC TAATTCCGGT 
CTTCCTGAGA CAAATGGACG CAAGCGCAAA AGCAGATGGG ACCAGCCGTC TAAGACAAAA 
GAACAAAGAA TCATGACTAT CTTGTCCCAA CAAACAGATG AAACTAATGG AAATCAGGAT 
GTCCAAGATG ACCTTCCACC CGGGTTTTCA TCACCCTGCA CAGATGTGCC TGATGCAATT 
ACTGCACAGC CGCAACAAAA GTTCCTTTCT CGCTTACCAG TTTCCTATGG GATTCCACTT 
AGCATTGTTC ATCAATTTGG TTCACCTGGC AAAGAGGACC CGACCACCTG GTCTGTTGCT 
CCTGGCATGC CGTTCTATCC ATTTCCACCT CTACCGCCAG TGTCTCATGG TGAGTTTTTT 
GCTAAGAGAA ACGTAAGAGC CTGTTCCTCG TCCATGGGAA ACCTGACTTA CTCCAATGAG 
ATCTTACCAG CTACACCTGT GACTGACTCG ACCGCTCCAA CCCGTAAGAG AGAGTTGTTT 
TCATCTGATA TAGGGACAAC TTACTTTCGG CAACAGAAAC AGAGTGTTCC TCCATGGTTG 
CGGAACAATG GGGGGGAGAA AACAGCAAAC AGCCCTATAC CTGGAAATCT AACTTTAGAG 
AAGAAGCTCA ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_177854.6
CDS	288..5705
Translation	MDCKENGVGDASGCNIDANSLASNLAMNTNEDFYEKLSSRGQNLDSVSSLEIPQTASSVNHTIEGQRKCFTEIEQMGYGNSNSQEDAGNTDDDLYVCYNADDTQEQGVVSGELEQSQELICDTDLLVNCNKLDDGKESQDTNVSLVSIFSGSMQEKEAPQAKEDEGYGGTTLPIGGSGIDTESTFVNDAPEQFESLETTKHIKPDEVESDGISYRFDDGGKEGRNGPSSDLDTGSSDDISLSQSFSFPDSLLDSSVFGCSATESYLEDAIDIEGNGTIVVSPSLAITEMLNNDDGGLCSHDLNKITVTETINPDLKLVREDRLDTDLSVMNEKMLKNHVGDSSSESAVAALSMNNGMAADLRAENFSQSSPIDEKTLDMEANSPITDSSLIWNFPLNFGSGGIEVCNPENAVEPLRIVDDNGRIGGEVASASGSDFCEAGMSSSRRKARDGKQCKVVQTKTSARHLRKSSRKKQSERDIESIFKCSKQKRSSLLKTSRSSEWGLPSKTTEIFLQSNNIPYDGPPHHEPQRSQGNLNNGEHNRSSHNGNVEGSNRNIQASSGSCLRLKVKFGKSGGQNPLNITVSKVSGNSLPGNGIVKAGTCLELPGSAHFGEDKMQTVETKEDLVEKSNPVEKVSYLQSSDSMRDKKYNQDAGGLCRKVGGDVLDDDPHLSSIRMVEECERATGTQSLDAETSPDSEVINSVPDSIVNIEHKEGLHHGFFSTPEDVVKKNRVLEKEDELRASKSPSENGSHLIPNAKKAKHPKSKSNGTKKGKSKFSESAKDGRKNESHEGVEQRKSLNTSMGRDDSDYPEVGRIESHKTTGALLDADIGKTSATYGTISSDVTHGEMVVDVTIEDSYSTESAWVRCDDCFKWRRIPASVVGSIDESSRWICMNNSDKRFADCSKSQEMSNEEINEELGIGQDEADAYDCDAAKRGKEKEQKSKRLTGKQKACFKAIKTNQFLHRNRKSQTIDEIMVCHCKPSPDGRLGCGEECLNRMLNIECLQGTCPAGDLCSNQQFQKRKYVKFERFQSGKKGYGLRLLEDVREGQFLIEYVGEVLDMQSYETRQKEYAFKGQKHFYFMTLNGNEVIDAGAKGNLGRFINHSCEPNCRTEKWMVNGEICVGIFSMQDLKKGQELTFDYNYVRVFGAAAKKCYCGSSHCRGYIGGDPLNGDVIIQSDSDEEYPELVILDDDESGEGILGATSRTFTDDADEQMPQSFEKVNGYKDLAPDNTQTQSSVSVKLPEREIPPPLLQPTEVLKELSSGISITAVQQEVPAEKKTKSTSPTSSSLSRMSPGGTNSDKTTKHGSGEDKKILPRPRPRMKTSRSSESSKRDKGGIYPGVNKAQVIPVNKLQQQPIKSKGSEKVSPSIETFEGKLNELLDAVGGISKRRDSAKGYLKLLLLTAASRGTDEEGIYSNRDLSMILDALLKTKSKSVLVDIINKNGLQMLHNIMKQYRGDFKRIPIIRKLLKVLEYLATRKILALEHIIRRPPFAGMESFKDSVLSFTEHDDYTVHNIARSFRDRWIPKHFRKPWRINREERSESMRSPINRRFRASQEPRYDHQSPRPAEPAASVTSSKAATPETASVSEGYSEPNSGLPETNGRKRKSRWDQPSKTKEQRIMTILSQQTDETNGNQDVQDDLPPGFSSPCTDVPDAITAQPQQKFLSRLPVSYGIPLSIVHQFGSPGKEDPTTWSVAPGMPFYPFPPLPPVSHGEFFAKRNVRACSSSMGNLTYSNEILPATPVTDSTAPTRKRELFSSDIGTTYFRQQKQSVPPWLRNNGGEKTANSPIPGNLTLEKKLNS

Target ORF information:

RefSeq Version	NM_106379.9
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana histone-lysine N-methyltransferase (EFS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_106379.9

ORF Insert Sequence:

ATGGACTGTA AGGAAAACGG TGTTGGTGAC GCTTCTGGGT GCAATATTGA TGCTAACTCT 
CTGGCCTCAA ATCTTGCGAT GAATACCAAT GAAGATTTCT ATGAGAAATT GAGTTCTCGT 
GGGCAAAATT TAGATTCAGT CAGTAGTTTG GAGATTCCTC AGACTGCTTC ATCTGTAAAC 
CATACGATTG AAGGCCAAAG GAAGTGCTTT ACAGAGATTG AGCAGATGGG ATACGGGAAC 
AGTAATAGTC AAGAAGATGC TGGAAACACT GATGATGATC TATATGTTTG TTACAATGCT 
GATGACACTC AGGAGCAGGG AGTGGTTTCA GGTGAGCTTG AACAAAGTCA AGAATTAATT 
TGTGATACTG ATTTACTGGT AAATTGCAAC AAGCTGGATG ATGGAAAGGA AAGTCAGGAC 
ACAAATGTGT CCCTTGTATC CATTTTTTCT GGGAGTATGC AAGAGAAAGA AGCTCCTCAG 
GCTAAAGAAG ATGAAGGTTA TGGTGGCACA ACATTGCCTA TAGGGGGCAG TGGAATAGAT 
ACGGAATCAA CTTTTGTGAA TGATGCACCA GAACAATTCG AGTCTTTGGA AACCACAAAG 
CACATAAAAC CTGATGAAGT TGAAAGTGAT GGTATTAGTT ATAGGTTTGA TGATGGAGGT 
AAGGAAGGAA GAAATGGACC ATCGAGTGAT CTAGATACTG GTTCTTCTGA TGATATATCT 
CTCAGTCAGA GTTTTTCGTT TCCAGATTCG TTATTGGACT CTAGTGTATT TGGCTGTAGC 
GCCACAGAAA GTTATCTGGA GGATGCAATT GATATTGAAG GCAATGGGAC TATTGTTGTA 
TCCCCTTCAT TAGCTATCAC AGAAATGTTA AATAATGATG ATGGTGGCCT ATGTTCACAT 
GATCTTAACA AGATCACAGT CACAGAGACG ATCAATCCAG ATTTGAAGTT GGTACGTGAG 
GATAGGCTTG ACACTGATCT TTCCGTGATG AATGAAAAGA TGCTAAAAAA CCATGTTGGG 
GATTCATCAA GTGAAAGTGC CGTGGCCGCT TTGAGTATGA ATAATGGTAT GGCTGCTGAT 
CTGAGAGCTG AAAATTTCAG TCAGAGTTCA CCCATAGACG AAAAAACTTT AGATATGGAA 
GCCAATTCCC CTATTACTGA CTCTTCTTTG ATATGGAATT TTCCATTGAA CTTTGGAAGT 
GGAGGAATCG AAGTCTGTAA TCCTGAAAAT GCAGTTGAGC CACTCAGAAT AGTAGATGAC 
AATGGCAGAA TAGGTGGTGA AGTTGCTTCT GCATCAGGGA GTGATTTTTG TGAAGCTGGC 
ATGTCTTCTT CCCGAAGGAA GGCCCGAGAC GGGAAACAAT GTAAAGTGGT GCAGACAAAA 
ACGAGTGCTC GCCATCTGAG AAAATCCTCT AGAAAGAAAC AATCAGAAAG AGACATTGAG 
TCAATTTTCA AGTGCTCAAA GCAGAAGAGG AGCTCTCTTT TAAAAACAAG TCGTTCATCT 
GAGTGGGGAT TACCAAGCAA GACCACTGAA ATCTTCTTAC AGAGCAATAA TATTCCCTAT 
GATGGACCTC CGCATCATGA ACCACAGAGA TCTCAGGGAA ATCTTAATAA TGGAGAGCAT 
AATAGAAGTT CTCATAACGG GAATGTAGAA GGATCTAACA GAAACATCCA AGCATCAAGT 
GGCTCTTGCC TTCGTTTGAA AGTTAAATTT GGTAAATCAG GTGGCCAAAA TCCTCTGAAC 
ATTACAGTCT CTAAGGTCAG TGGTAACTCC TTACCTGGTA ATGGTATTGT AAAAGCAGGA 
ACATGTTTAG AATTGCCAGG ATCAGCACAT TTTGGTGAGG ATAAAATGCA AACTGTGGAA 
ACTAAAGAGG ACTTGGTAGA GAAAAGCAAC CCTGTGGAGA AAGTTTCATA TCTTCAGTCA 
TCTGATTCTA TGAGGGATAA GAAATATAAC CAAGACGCTG GGGGTTTATG TAGGAAGGTG 
GGTGGTGATG TTTTAGATGA TGACCCACAC CTTTCCTCTA TTAGAATGGT TGAGGAGTGC 
GAGAGGGCCA CTGGAACGCA GTCCCTGGAC GCTGAAACTT CACCAGATTC TGAAGTTATC 
AACTCTGTGC CTGATTCCAT TGTCAATATT GAACATAAAG AGGGTCTGCA TCATGGTTTT 
TTTAGCACTC CAGAAGATGT GGTCAAGAAG AACAGAGTAT TAGAAAAAGA AGATGAATTG 
CGTGCTTCAA AATCTCCTTC AGAAAATGGT TCACATCTAA TTCCCAATGC AAAAAAAGCT 
AAACATCCGA AGTCTAAAAG TAATGGAACA AAGAAAGGTA AATCCAAGTT TTCCGAATCT 
GCCAAAGACG GGAGAAAAAA TGAATCACAT GAAGGGGTAG AGCAACGCAA ATCCCTGAAT 
ACCAGTATGG GAAGGGATGA TAGTGATTAT CCTGAAGTAG GGAGAATAGA GTCTCACAAA 
ACAACAGGTG CCCTTCTAGA TGCTGATATT GGGAAAACCA GTGCCACTTA TGGTACCATA 
TCATCAGATG TGACCCATGG GGAAATGGTT GTGGATGTTA CTATTGAAGA TAGCTATTCC 
ACAGAGAGTG CCTGGGTTCG ATGTGATGAT TGCTTTAAAT GGCGACGAAT ACCTGCTTCT 
GTTGTAGGAT CAATTGACGA GAGCTCTAGA TGGATCTGTA TGAACAACTC AGATAAAAGA 
TTTGCGGATT GCTCAAAATC TCAAGAGATG TCAAATGAAG AAATTAATGA AGAGTTGGGC 
ATAGGACAGG ATGAAGCAGA TGCATATGAT TGTGATGCGG CTAAAAGAGG GAAAGAAAAG 
GAACAGAAGA GCAAGCGTTT GACAGGTAAG CAAAAGGCGT GCTTCAAGGC TATAAAAACA 
AACCAGTTCC TTCATCGCAA TCGTAAATCT CAAACAATTG ACGAGATAAT GGTTTGTCAC 
TGCAAACCAT CACCTGATGG TAGGTTGGGT TGTGGAGAAG AATGCCTCAA TAGAATGCTT 
AACATTGAAT GTCTTCAAGG TACCTGTCCA GCTGGCGATT TGTGTTCAAA TCAGCAGTTT 
CAAAAACGGA AGTATGTTAA GTTTGAGAGA TTCCAATCCG GTAAGAAGGG TTATGGCCTG 
AGATTGCTCG AGGATGTACG AGAGGGGCAA TTCCTAATTG AATATGTTGG AGAGGTGCTT 
GATATGCAAT CCTATGAGAC TCGCCAAAAA GAATATGCTT TCAAGGGTCA GAAACATTTC 
TATTTCATGA CACTAAATGG GAATGAGGTA ATAGATGCTG GTGCAAAGGG AAACCTAGGG 
CGTTTCATTA ACCATAGCTG TGAACCAAAC TGCCGTACTG AAAAGTGGAT GGTGAATGGT 
GAAATTTGCG TTGGAATATT CTCCATGCAA GACCTTAAGA AGGGTCAAGA GTTGACATTT 
GACTACAACT ATGTGAGGGT TTTTGGTGCT GCCGCCAAAA AGTGCTATTG TGGATCATCA 
CATTGCAGAG GGTATATTGG GGGAGATCCT CTGAATGGTG ATGTAATTAT TCAAAGTGAT 
TCAGATGAAG AGTATCCTGA ACTTGTGATC CTTGATGATG ATGAAAGTGG AGAAGGAATC 
TTAGGTGCAA CATCTAGGAC CTTCACTGAT GACGCTGACG AGCAAATGCC ACAGAGCTTT 
GAAAAGGTTA ATGGTTACAA GGACCTTGCT CCTGATAATA CCCAAACACA GAGCTCAGTA 
TCTGTAAAAC TTCCAGAGAG AGAAATTCCT CCACCTCTTC TTCAGCCAAC TGAAGTTTTG 
AAGGAACTTT CGTCAGGCAT ATCTATTACT GCTGTCCAGC AAGAGGTTCC CGCTGAAAAG 
AAGACTAAAA GCACATCTCC CACGTCCAGT TCTCTTAGCA GAATGTCCCC GGGTGGTACA 
AATTCTGATA AGACGACAAA GCATGGATCA GGAGAAGATA AAAAGATACT TCCACGTCCT 
CGTCCTCGTA TGAAAACTTC TCGCTCATCT GAGTCTAGTA AGCGAGACAA AGGAGGTATT 
TATCCTGGTG TTAACAAAGC ACAGGTTATA CCAGTTAATA AGTTGCAACA GCAGCCCATC 
AAATCTAAAG GATCAGAGAA AGTTTCTCCC AGTATCGAAA CATTTGAAGG GAAACTGAAC 
GAGTTACTAG ATGCCGTAGG AGGGATAAGC AAGCGGAGGG ATTCAGCAAA AGGCTACTTG 
AAACTTCTGC TGCTCACTGC CGCTTCGCGA GGCACGGATG AAGAAGGAAT TTATAGCAAT 
CGAGATCTTT CAATGATTCT TGATGCCCTT TTGAAGACAA AGTCAAAATC TGTTTTAGTG 
GACATAATCA ACAAGAATGG TCTGCAAATG TTACATAATA TCATGAAACA ATACCGGGGT 
GATTTTAAAA GGATCCCTAT AATCCGGAAA CTTCTGAAGG TATTAGAGTA TCTTGCCACA 
AGGAAAATCC TTGCACTGGA GCATATAATC AGAAGGCCTC CTTTTGCAGG GATGGAAAGC 
TTTAAGGACT CTGTTCTATC ATTCACCGAG CATGACGACT ATACGGTTCA TAATATTGCG 
CGGAGCTTCC GAGACAGATG GATACCTAAG CATTTTAGAA AACCTTGGCG CATCAACAGG 
GAGGAGAGAT CGGAGTCTAT GAGGTCACCT ATAAACAGAA GGTTCAGAGC ATCACAAGAA 
CCTCGATATG ATCATCAGTC CCCAAGACCT GCAGAACCAG CTGCGTCTGT CACATCATCA 
AAGGCTGCAA CTCCTGAAAC AGCATCTGTA TCTGAGGGAT ATTCAGAACC TAATTCCGGT 
CTTCCTGAGA CAAATGGACG CAAGCGCAAA AGCAGATGGG ACCAGCCGTC TAAGACAAAA 
GAACAAAGAA TCATGACTAT CTTGTCCCAA CAAACAGATG AAACTAATGG AAATCAGGAT 
GTCCAAGATG ACCTTCCACC CGGGTTTTCA TCACCCTGCA CAGATGTGCC TGATGCAATT 
ACTGCACAGC CGCAACAAAA GTTCCTTTCT CGCTTACCAG TTTCCTATGG GATTCCACTT 
AGCATTGTTC ATCAATTTGG TTCACCTGGC AAAGAGGACC CGACCACCTG GTCTGTTGCT 
CCTGGCATGC CGTTCTATCC ATTTCCACCT CTACCGCCAG TGTCTCATGG TGAGTTTTTT 
GCTAAGAGAA ACGTAAGAGC CTGTTCCTCG TCCATGGGAA ACCTGACTTA CTCCAATGAG 
ATCTTACCAG CTACACCTGT GACTGACTCG ACCGCTCCAA CCCGTAAGAG AGAGTTGTTT 
TCATCTGATA TAGGGACAAC TTACTTTCGG CAACAGAAAC AGAGTGTTCC TCCATGGTTG 
CGGAACAATG GGGGGGAGAA AACAGCAAAC AGCCCTATAC CTGGAAATCT AACTTTAGAG 
AAGAAGCTCA ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( NM_001084367.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001077836.1 Arabidopsis thaliana histone-lysine N-methyltransferase (EFS), mRNA.

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CloneID	OAb28209
Clone ID Related Accession (Same CDS sequence)	NM_001084367.2
Accession Version	NM_001084367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	histone-lysine N-methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On May 26, 2011 this sequence version replaced NM_001084367.1.

ATGGACTGTA AGGAAAACGG TGTTGGTGAC GCTTCTGGGT GCAATATTGA TGCTAACTCT 
CTGGCCTCAA ATCTTGCGAT GAATACCAAT GAAGATTTCT ATGAGAAATT GAGTTCTCGT 
GGGCAAAATT TAGATTCAGT CAGTAGTTTG GAGATTCCTC AGACTGCTTC ATCTGTAAAC 
CATACGATTG AAGGCCAAAG GAAGTGCTTT ACAGAGATTG AGCAGATGGG ATACGGGAAC 
AGTAATAGTC AAGAAGATGC TGGAAACACT GATGATGATC TATATGTTTG TTACAATGCT 
GATGACACTC AGGAGCAGGG AGTGGTTTCA GGTGAGCTTG AACAAAGTCA AGAATTAATT 
TGTGATACTG ATTTACTGGT AAATTGCAAC AAGCTGGATG ATGGAAAGGA AAGTCAGGAC 
ACAAATGTGT CCCTTGTATC CATTTTTTCT GGGAGTATGC AAGAGAAAGA AGCTCCTCAG 
GCTAAAGAAG ATGAAGGTTA TGGTGGCACA ACATTGCCTA TAGGGGGCAG TGGAATAGAT 
ACGGAATCAA CTTTTGTGAA TGATGCACCA GAACAATTCG AGTCTTTGGA AACCACAAAG 
CACATAAAAC CTGATGAAGT TGAAAGTGAT GGTATTAGTT ATAGGTTTGA TGATGGAGGT 
AAGGAAGGAA GAAATGGACC ATCGAGTGAT CTAGATACTG GTTCTTCTGA TGATATATCT 
CTCAGTCAGA GTTTTTCGTT TCCAGATTCG TTATTGGACT CTAGTGTATT TGGCTGTAGC 
GCCACAGAAA GTTATCTGGA GGATGCAATT GATATTGAAG GCAATGGGAC TATTGTTGTA 
TCCCCTTCAT TAGCTATCAC AGAAATGTTA AATAATGATG ATGGTGGCCT ATGTTCACAT 
GATCTTAACA AGATCACAGT CACAGAGACG ATCAATCCAG ATTTGAAGTT GGTACGTGAG 
GATAGGCTTG ACACTGATCT TTCCGTGATG AATGAAAAGA TGCTAAAAAA CCATGTTGGG 
GATTCATCAA GTGAAAGTGC CGTGGCCGCT TTGAGTATGA ATAATGGTAT GGCTGCTGAT 
CTGAGAGCTG AAAATTTCAG TCAGAGTTCA CCCATAGACG AAAAAACTTT AGATATGGAA 
GCCAATTCCC CTATTACTGA CTCTTCTTTG ATATGGAATT TTCCATTGAA CTTTGGAAGT 
GGAGGAATCG AAGTCTGTAA TCCTGAAAAT GCAGTTGAGC CACTCAGAAT AGTAGATGAC 
AATGGCAGAA TAGGTGGTGA AGTTGCTTCT GCATCAGGGA GTGATTTTTG TGAAGCTGGC 
ATGTCTTCTT CCCGAAGGAA GGCCCGAGAC GGGAAACAAT GTAAAGTGGT GCAGACAAAA 
ACGAGTGCTC GCCATCTGAG AAAATCCTCT AGAAAGAAAC AATCAGAAAG AGACATTGAG 
TCAATTTTCA AGTGCTCAAA GCAGAAGAGG AGCTCTCTTT TAAAAACAAG TCGTTCATCT 
GAGTGGGGAT TACCAAGCAA GACCACTGAA ATCTTCTTAC AGAGCAATAA TATTCCCTAT 
GATGGACCTC CGCATCATGA ACCACAGAGA TCTCAGGGAA ATCTTAATAA TGGAGAGCAT 
AATAGAAGTT CTCATAACGG GAATGTAGAA GGATCTAACA GAAACATCCA AGCATCAAGT 
GGCTCTTGCC TTCGTTTGAA AGTTAAATTT GGTAAATCAG GTGGCCAAAA TCCTCTGAAC 
ATTACAGTCT CTAAGGTCAG TGGTAACTCC TTACCTGGTA ATGGTATTGT AAAAGCAGGA 
ACATGTTTAG AATTGCCAGG ATCAGCACAT TTTGGTGAGG ATAAAATGCA AACTGTGGAA 
ACTAAAGAGG ACTTGGTAGA GAAAAGCAAC CCTGTGGAGA AAGTTTCATA TCTTCAGTCA 
TCTGATTCTA TGAGGGATAA GAAATATAAC CAAGACGCTG GGGGTTTATG TAGGAAGGTG 
GGTGGTGATG TTTTAGATGA TGACCCACAC CTTTCCTCTA TTAGAATGGT TGAGGAGTGC 
GAGAGGGCCA CTGGAACGCA GTCCCTGGAC GCTGAAACTT CACCAGATTC TGAAGTTATC 
AACTCTGTGC CTGATTCCAT TGTCAATATT GAACATAAAG AGGGTCTGCA TCATGGTTTT 
TTTAGCACTC CAGAAGATGT GGTCAAGAAG AACAGAGTAT TAGAAAAAGA AGATGAATTG 
CGTGCTTCAA AATCTCCTTC AGAAAATGGT TCACATCTAA TTCCCAATGC AAAAAAAGCT 
AAACATCCGA AGTCTAAAAG TAATGGAACA AAGAAAGGTA AATCCAAGTT TTCCGAATCT 
GCCAAAGACG GGAGAAAAAA TGAATCACAT GAAGGGGTAG AGCAACGCAA ATCCCTGAAT 
ACCAGTATGG GAAGGGATGA TAGTGATTAT CCTGAAGTAG GGAGAATAGA GTCTCACAAA 
ACAACAGGTG CCCTTCTAGA TGCTGATATT GGGAAAACCA GTGCCACTTA TGGTACCATA 
TCATCAGATG TGACCCATGG GGAAATGGTT GTGGATGTTA CTATTGAAGA TAGCTATTCC 
ACAGAGAGTG CCTGGGTTCG ATGTGATGAT TGCTTTAAAT GGCGACGAAT ACCTGCTTCT 
GTTGTAGGAT CAATTGACGA GAGCTCTAGA TGGATCTGTA TGAACAACTC AGATAAAAGA 
TTTGCGGATT GCTCAAAATC TCAAGAGATG TCAAATGAAG AAATTAATGA AGAGTTGGGC 
ATAGGACAGG ATGAAGCAGA TGCATATGAT TGTGATGCGG CTAAAAGAGG GAAAGAAAAG 
GAACAGAAGA GCAAGCGTTT GACAGGTAAG CAAAAGGCGT GCTTCAAGGC TATAAAAACA 
AACCAGTTCC TTCATCGCAA TCGTAAATCT CAAACAATTG ACGAGATAAT GGTTTGTCAC 
TGCAAACCAT CACCTGATGG TAGGTTGGGT TGTGGAGAAG AATGCCTCAA TAGAATGCTT 
AACATTGAAT GTCTTCAAGG TACCTGTCCA GCTGGCGATT TGTGTTCAAA TCAGCAGTTT 
CAAAAACGGA AGTATGTTAA GTTTGAGAGA TTCCAATCCG GTAAGAAGGG TTATGGCCTG 
AGATTGCTCG AGGATGTACG AGAGGGGCAA TTCCTAATTG AATATGTTGG AGAGGTGCTT 
GATATGCAAT CCTATGAGAC TCGCCAAAAA GAATATGCTT TCAAGGGTCA GAAACATTTC 
TATTTCATGA CACTAAATGG GAATGAGGTA ATAGATGCTG GTGCAAAGGG AAACCTAGGG 
CGTTTCATTA ACCATAGCTG TGAACCAAAC TGCCGTACTG AAAAGTGGAT GGTGAATGGT 
GAAATTTGCG TTGGAATATT CTCCATGCAA GACCTTAAGA AGGGTCAAGA GTTGACATTT 
GACTACAACT ATGTGAGGGT TTTTGGTGCT GCCGCCAAAA AGTGCTATTG TGGATCATCA 
CATTGCAGAG GGTATATTGG GGGAGATCCT CTGAATGGTG ATGTAATTAT TCAAAGTGAT 
TCAGATGAAG AGTATCCTGA ACTTGTGATC CTTGATGATG ATGAAAGTGG AGAAGGAATC 
TTAGGTGCAA CATCTAGGAC CTTCACTGAT GACGCTGACG AGCAAATGCC ACAGAGCTTT 
GAAAAGGTTA ATGGTTACAA GGACCTTGCT CCTGATAATA CCCAAACACA GAGCTCAGTA 
TCTGTAAAAC TTCCAGAGAG AGAAATTCCT CCACCTCTTC TTCAGCCAAC TGAAGTTTTG 
AAGGAACTTT CGTCAGGCAT ATCTATTACT GCTGTCCAGC AAGAGGTTCC CGCTGAAAAG 
AAGACTAAAA GCACATCTCC CACGTCCAGT TCTCTTAGCA GAATGTCCCC GGGTGGTACA 
AATTCTGATA AGACGACAAA GCATGGATCA GGAGAAGATA AAAAGATACT TCCACGTCCT 
CGTCCTCGTA TGAAAACTTC TCGCTCATCT GAGTCTAGTA AGCGAGACAA AGGAGGTATT 
TATCCTGGTG TTAACAAAGC ACAGGTTATA CCAGTTAATA AGTTGCAACA GCAGCCCATC 
AAATCTAAAG GATCAGAGAA AGTTTCTCCC AGTATCGAAA CATTTGAAGG GAAACTGAAC 
GAGTTACTAG ATGCCGTAGG AGGGATAAGC AAGCGGAGGG ATTCAGCAAA AGGCTACTTG 
AAACTTCTGC TGCTCACTGC CGCTTCGCGA GGCACGGATG AAGAAGGAAT TTATAGCAAT 
CGAGATCTTT CAATGATTCT TGATGCCCTT TTGAAGACAA AGTCAAAATC TGTTTTAGTG 
GACATAATCA ACAAGAATGG CCTCCTTTTG CAGGGATGGA AAGCTTTAAG GACTCTGTTC 
TATCATTCAC CGAGCATGAC GACTATACGG TTCATAATAT TGCGCGGAGC TTCCGAGACA 
GATGGATACC TAAGCATTTT AGAAAACCTT GGCGCATCAA CAGGGAGGAG AGATCGGAGT 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077836.1
CDS	136..4641
Translation	MDCKENGVGDASGCNIDANSLASNLAMNTNEDFYEKLSSRGQNLDSVSSLEIPQTASSVNHTIEGQRKCFTEIEQMGYGNSNSQEDAGNTDDDLYVCYNADDTQEQGVVSGELEQSQELICDTDLLVNCNKLDDGKESQDTNVSLVSIFSGSMQEKEAPQAKEDEGYGGTTLPIGGSGIDTESTFVNDAPEQFESLETTKHIKPDEVESDGISYRFDDGGKEGRNGPSSDLDTGSSDDISLSQSFSFPDSLLDSSVFGCSATESYLEDAIDIEGNGTIVVSPSLAITEMLNNDDGGLCSHDLNKITVTETINPDLKLVREDRLDTDLSVMNEKMLKNHVGDSSSESAVAALSMNNGMAADLRAENFSQSSPIDEKTLDMEANSPITDSSLIWNFPLNFGSGGIEVCNPENAVEPLRIVDDNGRIGGEVASASGSDFCEAGMSSSRRKARDGKQCKVVQTKTSARHLRKSSRKKQSERDIESIFKCSKQKRSSLLKTSRSSEWGLPSKTTEIFLQSNNIPYDGPPHHEPQRSQGNLNNGEHNRSSHNGNVEGSNRNIQASSGSCLRLKVKFGKSGGQNPLNITVSKVSGNSLPGNGIVKAGTCLELPGSAHFGEDKMQTVETKEDLVEKSNPVEKVSYLQSSDSMRDKKYNQDAGGLCRKVGGDVLDDDPHLSSIRMVEECERATGTQSLDAETSPDSEVINSVPDSIVNIEHKEGLHHGFFSTPEDVVKKNRVLEKEDELRASKSPSENGSHLIPNAKKAKHPKSKSNGTKKGKSKFSESAKDGRKNESHEGVEQRKSLNTSMGRDDSDYPEVGRIESHKTTGALLDADIGKTSATYGTISSDVTHGEMVVDVTIEDSYSTESAWVRCDDCFKWRRIPASVVGSIDESSRWICMNNSDKRFADCSKSQEMSNEEINEELGIGQDEADAYDCDAAKRGKEKEQKSKRLTGKQKACFKAIKTNQFLHRNRKSQTIDEIMVCHCKPSPDGRLGCGEECLNRMLNIECLQGTCPAGDLCSNQQFQKRKYVKFERFQSGKKGYGLRLLEDVREGQFLIEYVGEVLDMQSYETRQKEYAFKGQKHFYFMTLNGNEVIDAGAKGNLGRFINHSCEPNCRTEKWMVNGEICVGIFSMQDLKKGQELTFDYNYVRVFGAAAKKCYCGSSHCRGYIGGDPLNGDVIIQSDSDEEYPELVILDDDESGEGILGATSRTFTDDADEQMPQSFEKVNGYKDLAPDNTQTQSSVSVKLPEREIPPPLLQPTEVLKELSSGISITAVQQEVPAEKKTKSTSPTSSSLSRMSPGGTNSDKTTKHGSGEDKKILPRPRPRMKTSRSSESSKRDKGGIYPGVNKAQVIPVNKLQQQPIKSKGSEKVSPSIETFEGKLNELLDAVGGISKRRDSAKGYLKLLLLTAASRGTDEEGIYSNRDLSMILDALLKTKSKSVLVDIINKNGLLLQGWKALRTLFYHSPSMTTIRFIILRGASETDGYLSILENLGASTGRRDRSL

Target ORF information:

RefSeq Version	NM_001084367.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana histone-lysine N-methyltransferase (EFS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084367.2

ORF Insert Sequence:

ATGGACTGTA AGGAAAACGG TGTTGGTGAC GCTTCTGGGT GCAATATTGA TGCTAACTCT 
CTGGCCTCAA ATCTTGCGAT GAATACCAAT GAAGATTTCT ATGAGAAATT GAGTTCTCGT 
GGGCAAAATT TAGATTCAGT CAGTAGTTTG GAGATTCCTC AGACTGCTTC ATCTGTAAAC 
CATACGATTG AAGGCCAAAG GAAGTGCTTT ACAGAGATTG AGCAGATGGG ATACGGGAAC 
AGTAATAGTC AAGAAGATGC TGGAAACACT GATGATGATC TATATGTTTG TTACAATGCT 
GATGACACTC AGGAGCAGGG AGTGGTTTCA GGTGAGCTTG AACAAAGTCA AGAATTAATT 
TGTGATACTG ATTTACTGGT AAATTGCAAC AAGCTGGATG ATGGAAAGGA AAGTCAGGAC 
ACAAATGTGT CCCTTGTATC CATTTTTTCT GGGAGTATGC AAGAGAAAGA AGCTCCTCAG 
GCTAAAGAAG ATGAAGGTTA TGGTGGCACA ACATTGCCTA TAGGGGGCAG TGGAATAGAT 
ACGGAATCAA CTTTTGTGAA TGATGCACCA GAACAATTCG AGTCTTTGGA AACCACAAAG 
CACATAAAAC CTGATGAAGT TGAAAGTGAT GGTATTAGTT ATAGGTTTGA TGATGGAGGT 
AAGGAAGGAA GAAATGGACC ATCGAGTGAT CTAGATACTG GTTCTTCTGA TGATATATCT 
CTCAGTCAGA GTTTTTCGTT TCCAGATTCG TTATTGGACT CTAGTGTATT TGGCTGTAGC 
GCCACAGAAA GTTATCTGGA GGATGCAATT GATATTGAAG GCAATGGGAC TATTGTTGTA 
TCCCCTTCAT TAGCTATCAC AGAAATGTTA AATAATGATG ATGGTGGCCT ATGTTCACAT 
GATCTTAACA AGATCACAGT CACAGAGACG ATCAATCCAG ATTTGAAGTT GGTACGTGAG 
GATAGGCTTG ACACTGATCT TTCCGTGATG AATGAAAAGA TGCTAAAAAA CCATGTTGGG 
GATTCATCAA GTGAAAGTGC CGTGGCCGCT TTGAGTATGA ATAATGGTAT GGCTGCTGAT 
CTGAGAGCTG AAAATTTCAG TCAGAGTTCA CCCATAGACG AAAAAACTTT AGATATGGAA 
GCCAATTCCC CTATTACTGA CTCTTCTTTG ATATGGAATT TTCCATTGAA CTTTGGAAGT 
GGAGGAATCG AAGTCTGTAA TCCTGAAAAT GCAGTTGAGC CACTCAGAAT AGTAGATGAC 
AATGGCAGAA TAGGTGGTGA AGTTGCTTCT GCATCAGGGA GTGATTTTTG TGAAGCTGGC 
ATGTCTTCTT CCCGAAGGAA GGCCCGAGAC GGGAAACAAT GTAAAGTGGT GCAGACAAAA 
ACGAGTGCTC GCCATCTGAG AAAATCCTCT AGAAAGAAAC AATCAGAAAG AGACATTGAG 
TCAATTTTCA AGTGCTCAAA GCAGAAGAGG AGCTCTCTTT TAAAAACAAG TCGTTCATCT 
GAGTGGGGAT TACCAAGCAA GACCACTGAA ATCTTCTTAC AGAGCAATAA TATTCCCTAT 
GATGGACCTC CGCATCATGA ACCACAGAGA TCTCAGGGAA ATCTTAATAA TGGAGAGCAT 
AATAGAAGTT CTCATAACGG GAATGTAGAA GGATCTAACA GAAACATCCA AGCATCAAGT 
GGCTCTTGCC TTCGTTTGAA AGTTAAATTT GGTAAATCAG GTGGCCAAAA TCCTCTGAAC 
ATTACAGTCT CTAAGGTCAG TGGTAACTCC TTACCTGGTA ATGGTATTGT AAAAGCAGGA 
ACATGTTTAG AATTGCCAGG ATCAGCACAT TTTGGTGAGG ATAAAATGCA AACTGTGGAA 
ACTAAAGAGG ACTTGGTAGA GAAAAGCAAC CCTGTGGAGA AAGTTTCATA TCTTCAGTCA 
TCTGATTCTA TGAGGGATAA GAAATATAAC CAAGACGCTG GGGGTTTATG TAGGAAGGTG 
GGTGGTGATG TTTTAGATGA TGACCCACAC CTTTCCTCTA TTAGAATGGT TGAGGAGTGC 
GAGAGGGCCA CTGGAACGCA GTCCCTGGAC GCTGAAACTT CACCAGATTC TGAAGTTATC 
AACTCTGTGC CTGATTCCAT TGTCAATATT GAACATAAAG AGGGTCTGCA TCATGGTTTT 
TTTAGCACTC CAGAAGATGT GGTCAAGAAG AACAGAGTAT TAGAAAAAGA AGATGAATTG 
CGTGCTTCAA AATCTCCTTC AGAAAATGGT TCACATCTAA TTCCCAATGC AAAAAAAGCT 
AAACATCCGA AGTCTAAAAG TAATGGAACA AAGAAAGGTA AATCCAAGTT TTCCGAATCT 
GCCAAAGACG GGAGAAAAAA TGAATCACAT GAAGGGGTAG AGCAACGCAA ATCCCTGAAT 
ACCAGTATGG GAAGGGATGA TAGTGATTAT CCTGAAGTAG GGAGAATAGA GTCTCACAAA 
ACAACAGGTG CCCTTCTAGA TGCTGATATT GGGAAAACCA GTGCCACTTA TGGTACCATA 
TCATCAGATG TGACCCATGG GGAAATGGTT GTGGATGTTA CTATTGAAGA TAGCTATTCC 
ACAGAGAGTG CCTGGGTTCG ATGTGATGAT TGCTTTAAAT GGCGACGAAT ACCTGCTTCT 
GTTGTAGGAT CAATTGACGA GAGCTCTAGA TGGATCTGTA TGAACAACTC AGATAAAAGA 
TTTGCGGATT GCTCAAAATC TCAAGAGATG TCAAATGAAG AAATTAATGA AGAGTTGGGC 
ATAGGACAGG ATGAAGCAGA TGCATATGAT TGTGATGCGG CTAAAAGAGG GAAAGAAAAG 
GAACAGAAGA GCAAGCGTTT GACAGGTAAG CAAAAGGCGT GCTTCAAGGC TATAAAAACA 
AACCAGTTCC TTCATCGCAA TCGTAAATCT CAAACAATTG ACGAGATAAT GGTTTGTCAC 
TGCAAACCAT CACCTGATGG TAGGTTGGGT TGTGGAGAAG AATGCCTCAA TAGAATGCTT 
AACATTGAAT GTCTTCAAGG TACCTGTCCA GCTGGCGATT TGTGTTCAAA TCAGCAGTTT 
CAAAAACGGA AGTATGTTAA GTTTGAGAGA TTCCAATCCG GTAAGAAGGG TTATGGCCTG 
AGATTGCTCG AGGATGTACG AGAGGGGCAA TTCCTAATTG AATATGTTGG AGAGGTGCTT 
GATATGCAAT CCTATGAGAC TCGCCAAAAA GAATATGCTT TCAAGGGTCA GAAACATTTC 
TATTTCATGA CACTAAATGG GAATGAGGTA ATAGATGCTG GTGCAAAGGG AAACCTAGGG 
CGTTTCATTA ACCATAGCTG TGAACCAAAC TGCCGTACTG AAAAGTGGAT GGTGAATGGT 
GAAATTTGCG TTGGAATATT CTCCATGCAA GACCTTAAGA AGGGTCAAGA GTTGACATTT 
GACTACAACT ATGTGAGGGT TTTTGGTGCT GCCGCCAAAA AGTGCTATTG TGGATCATCA 
CATTGCAGAG GGTATATTGG GGGAGATCCT CTGAATGGTG ATGTAATTAT TCAAAGTGAT 
TCAGATGAAG AGTATCCTGA ACTTGTGATC CTTGATGATG ATGAAAGTGG AGAAGGAATC 
TTAGGTGCAA CATCTAGGAC CTTCACTGAT GACGCTGACG AGCAAATGCC ACAGAGCTTT 
GAAAAGGTTA ATGGTTACAA GGACCTTGCT CCTGATAATA CCCAAACACA GAGCTCAGTA 
TCTGTAAAAC TTCCAGAGAG AGAAATTCCT CCACCTCTTC TTCAGCCAAC TGAAGTTTTG 
AAGGAACTTT CGTCAGGCAT ATCTATTACT GCTGTCCAGC AAGAGGTTCC CGCTGAAAAG 
AAGACTAAAA GCACATCTCC CACGTCCAGT TCTCTTAGCA GAATGTCCCC GGGTGGTACA 
AATTCTGATA AGACGACAAA GCATGGATCA GGAGAAGATA AAAAGATACT TCCACGTCCT 
CGTCCTCGTA TGAAAACTTC TCGCTCATCT GAGTCTAGTA AGCGAGACAA AGGAGGTATT 
TATCCTGGTG TTAACAAAGC ACAGGTTATA CCAGTTAATA AGTTGCAACA GCAGCCCATC 
AAATCTAAAG GATCAGAGAA AGTTTCTCCC AGTATCGAAA CATTTGAAGG GAAACTGAAC 
GAGTTACTAG ATGCCGTAGG AGGGATAAGC AAGCGGAGGG ATTCAGCAAA AGGCTACTTG 
AAACTTCTGC TGCTCACTGC CGCTTCGCGA GGCACGGATG AAGAAGGAAT TTATAGCAAT 
CGAGATCTTT CAATGATTCT TGATGCCCTT TTGAAGACAA AGTCAAAATC TGTTTTAGTG 
GACATAATCA ACAAGAATGG CCTCCTTTTG CAGGGATGGA AAGCTTTAAG GACTCTGTTC 
TATCATTCAC CGAGCATGAC GACTATACGG TTCATAATAT TGCGCGGAGC TTCCGAGACA 
GATGGATACC TAAGCATTTT AGAAAACCTT GGCGCATCAA CAGGGAGGAG AGATCGGAGT 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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EFS cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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