Tic20-I cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tic20-I
Entrez Gene ID	839374
Full Name	translocon at the inner envelope membrane of chloroplasts 20
Synonyms	AtTic20-I,F13M7.7,F13M7_7,TIC20,atTIC20,translocon at the inner envelope membrane of chloroplasts 20,translocon at the inner envelope membrane of chloroplasts 20-I
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_100372.5	NP_171986.3	translocon at the inner envelope membrane of chloroplasts 20

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT1G04945	NP_001184910.1
Arabidopsis thaliana (thale cress)	TIC20	NP_171986.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

atTic20 functions similarly to other small alpha-helical integral membrane proteins, such as Tim23, that are involved in protein transport across membranes.
Title: Folding and self-association of atTic20 in lipid membranes: implications for understanding protein transport across the inner envelope membrane of chloroplasts.

The inner envelope localized Tic20 proteins expose their C-termini to the chloroplast stroma.
Title: The localization of Tic20 proteins in Arabidopsis thaliana is not restricted to the inner envelope membrane of chloroplasts.

Tic20 protein is much less abundant than Tic110 in envelope membranes. Tic20 forms high molecular weight complexes separately from Tic110.
Title: Tic20 forms a channel independent of Tic110 in chloroplasts.

The tic20-I mutants exhibit albinism and severe defects in chloroplast biogenesis.
Title: Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts.

AtTic20-IV is expressed mainly in roots whereas AtTic20-I is more abundant in shoots than in roots.
Title: In vivo studies on the roles of two closely related Arabidopsis Tic20 proteins, AtTic20-I and AtTic20-IV.

The following Tic20-I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tic20-I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24698	NM_100372.5 Latest version!	Arabidopsis thaliana translocon at the inner envelope membrane of chloroplasts 20 (Tic20-I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tic20-I ( NM_100372.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_171986.3 Arabidopsis thaliana translocon at the inner envelope membrane of chloroplasts 20 (Tic20-I), mRNA.

Price & Availability↑

CloneID	OAb24698
Clone ID Related Accession (Same CDS sequence)	NM_100372.5
Accession Version	NM_100372.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the inner envelope membrane of chloroplasts 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100372.4.

ATGATAACTG GATACAGCAC GCCAAGTGCA CATGTTCTAA TGAGCTCTCG GGCATTCAAG 
TCATCATCAT ATAGAGCTGC AGCAGGACAG ACTCAACATT ATCTTGCTCG AAGTTCATTG 
CCTGTCGTAA AGAACTCGTG GGGATCACCA CCTTCACCTT TCAATGAGCT TCCGAGAGTG 
TCAAGAGGTG TGCCTCTGTC ATATCTCTCA GCCTCGTCTT CTCTGCTTCT GAATGGAGAA 
CAAGGTAGTC TATCTGGTAC ATTACCTGTG TTACCTGTCC GCAGAAAAAC TCTTTTGACT 
CCACGAGCGT CAAAAGATGT ACCTTCTAGC TTCCGATTTC CCCCGATGAC CAAGAAGCCA 
CAATGGTGGT GGAGAACTTT GGCTTGCCTG CCTTACCTAA TGCCACTGCA TGAAACTTGG 
ATGTATGCAG AAACCGCTTA CCATCTCCAC CCATTCCTAG AAGATTTTGA ATTCTTAACC 
TACCCATTTC TAGGCGCCAT AGGAAGATTA CCAAGCTGGT TCCTCATGGC TTACTTTTTT 
GTAGCTTATC TAGGGATAGT GCGAAGAAAA GAATGGCCTC ACTTCTTCAG GTTCCATGTA 
GTGATGGGTA TGCTGCTTGA AATCGCACTC CAGGTTATAG GGACCGTTAG CAAGTGGATG 
CCTCTTGGAG TCTATTGGGG TAAGTTTGGG ATGCATTTCT GGACTGCTGT TGCGTTTGCT 
TATCTGTTTA CCGTCCTTGA AAGCATACGG TGTGCACTTG CGGGTATGTA CGCAGACATC 
CCGTTTGTCT GTGATGCTGC CTATATCCAG ATTCCGTACG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_171986.3
CDS	160..984
Translation	MITGYSTPSAHVLMSSRAFKSSSYRAAAGQTQHYLARSSLPVVKNSWGSPPSPFNELPRVSRGVPLSYLSASSSLLLNGEQGSLSGTLPVLPVRRKTLLTPRASKDVPSSFRFPPMTKKPQWWWRTLACLPYLMPLHETWMYAETAYHLHPFLEDFEFLTYPFLGAIGRLPSWFLMAYFFVAYLGIVRRKEWPHFFRFHVVMGMLLEIALQVIGTVSKWMPLGVYWGKFGMHFWTAVAFAYLFTVLESIRCALAGMYADIPFVCDAAYIQIPYD

Target ORF information:

RefSeq Version	NM_100372.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the inner envelope membrane of chloroplasts 20 (Tic20-I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100372.5

ORF Insert Sequence:

ATGATAACTG GATACAGCAC GCCAAGTGCA CATGTTCTAA TGAGCTCTCG GGCATTCAAG 
TCATCATCAT ATAGAGCTGC AGCAGGACAG ACTCAACATT ATCTTGCTCG AAGTTCATTG 
CCTGTCGTAA AGAACTCGTG GGGATCACCA CCTTCACCTT TCAATGAGCT TCCGAGAGTG 
TCAAGAGGTG TGCCTCTGTC ATATCTCTCA GCCTCGTCTT CTCTGCTTCT GAATGGAGAA 
CAAGGTAGTC TATCTGGTAC ATTACCTGTG TTACCTGTCC GCAGAAAAAC TCTTTTGACT 
CCACGAGCGT CAAAAGATGT ACCTTCTAGC TTCCGATTTC CCCCGATGAC CAAGAAGCCA 
CAATGGTGGT GGAGAACTTT GGCTTGCCTG CCTTACCTAA TGCCACTGCA TGAAACTTGG 
ATGTATGCAG AAACCGCTTA CCATCTCCAC CCATTCCTAG AAGATTTTGA ATTCTTAACC 
TACCCATTTC TAGGCGCCAT AGGAAGATTA CCAAGCTGGT TCCTCATGGC TTACTTTTTT 
GTAGCTTATC TAGGGATAGT GCGAAGAAAA GAATGGCCTC ACTTCTTCAG GTTCCATGTA 
GTGATGGGTA TGCTGCTTGA AATCGCACTC CAGGTTATAG GGACCGTTAG CAAGTGGATG 
CCTCTTGGAG TCTATTGGGG TAAGTTTGGG ATGCATTTCT GGACTGCTGT TGCGTTTGCT 
TATCTGTTTA CCGTCCTTGA AAGCATACGG TGTGCACTTG CGGGTATGTA CGCAGACATC 
CCGTTTGTCT GTGATGCTGC CTATATCCAG ATTCCGTACG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Tic20-I cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報