BARD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	BARD1
Entrez Gene ID	839329
Full Name	breast cancer associated RING 1
Synonyms	ATBARD1,F21M11.4,F21M11_4,ROW1,breast cancer associated RING 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202029.2	NP_973758.2	breast cancer associated RING 1
NM_001331456.1	NP_001318917.1	breast cancer associated RING 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	BARD1	NP_171898.2
Oryza sativa (rice)	Os04g0512400	NP_001053298.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that the study of AtROW1 in plant may provide a model for elucidating the functional mechanism of BARD1 in mammals.
Title: Recent advances in the research for the homolog of breast cancer associated gene AtROW1 in higher plants.

ROW1 is essential for quiescent centre maintenance and for stem cell niche development through the repression of WOX5 in the proximal meristem.
Title: ROW1 maintains quiescent centre identity by confining WOX5 expression to specific cells.

Data suggest that BARD1 regulates shoot apical meristem organization and maintenance by limiting WUS expression to the organizing center.
Title: Mutation of Arabidopsis BARD1 causes meristem defects by failing to confine WUSCHEL expression to the organizing center.

The following BARD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BARD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24658	NM_202029.2 Latest version!	Arabidopsis thaliana breast cancer associated RING 1 (BARD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb32923	NM_001331456.1 Latest version!	Arabidopsis thaliana breast cancer associated RING 1 (BARD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BARD1 ( NM_202029.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_973758.2 Arabidopsis thaliana breast cancer associated RING 1 (BARD1), mRNA.

Price & Availability↑

CloneID	OAb24658
Clone ID Related Accession (Same CDS sequence)	NM_202029.2
Accession Version	NM_202029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	breast cancer associated RING 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Apr 18, 2007 this sequence version replaced NM_202029.1.

ATGGCGGAAT TTACTAACAT GCTAATGAAT CCATGGGTTC TTCATCTCCA GAAGCTCGAG 
CTCGAGCTCA AGTGCCCTCT ATGCTTAAAA TTGCTTAATC GACCGGTGTT GCTTCCTTGC 
GACCACGTCT TCTGCGATTC ATGTGTACAC AAATCGTCGC AAGTTGAATC GGGATGTCCT 
GTCTGCAAAT CTAAACATCC CAAAAAGGGT AAGAGAGATC TTCGATTCAT GGAGAGTGTT 
ATAAGCATAT ACAAGAGCTT GAATGCAGCT GTTAGTGTTC ATCTCCCACA ATTACAGATT 
CCAAATGATT GCAACTACAA GAACGATGCA TTGAACAATT CCAACAGCCC TAAACATGGC 
GAATCTGAAG ATTCTGAGAT GACTGACAAG GATGTGAGCA AGCGCAGTGG TGGTACTGAT 
TCTTCTAGTC GTGATGGTTC TCCTCTTCCG ACCTCAGAAG AAAGTGATCC AAGACCTAAG 
CATCAGGATT GGACTGAAAA GCAGTTGAGT GATCATCTCC TATTATATGA GTTTGAATCA 
GAGTATGATG CTGCCAATCA CACTCCGGAA AGTTATACAG AACAAGCTGC AAAAAATGTG 
AGGGACATCA CTGCTTCTGA GCAGCCTAGC AATGCTGCAA GGAAGCGGAT ATGTGGTGAT 
TCCTTTATCC AGGAAAGCAG TCCGAATCCT AAGACTCAGG ATCCGACTTT GCTGCGGTTG 
ATGGAGAGCC TCCGGTCAGA TGATCCCACT GATTATGTTA AAGCACAGAA CCATCAGTTA 
CCCAAAAGTC ATACAGAACA AGATTCAAAA AGGAAGCGTG ACATCACCGC TTCTGATGCA 
ATGGAAAATC ATCTGAAGGT CCCCAAGAGG GAGAATAATC TGATGCAAAA ATCTGCTGAT 
ATTGACTGCA ATGGTAAATG CTCTGCAAAC TCTGATGATC AGCTAAGTGA GAAAATCTCA 
AAGGCATTAG AACAAACATC ATCCAACATA ACCATTTGTG GTTTCTGCCA ATCCGCTAGG 
GTATCTGAGG CCACTGGAGA AATGCTGCAT TACTCTAGGG GAAGACCGGT GGATGGAGAT 
GACATCTTCC GCTCCAATGT TATCCATGTC CATAGCGCAT GTATCGAATG GGCACCGCAA 
GTTTACTATG AAGGTGACAC AGTGAAGAAC CTGAAAGCAG AACTGGCAAG GGGGATGAAA 
ATCAAGTGCA CTAAATGTAG TCTGAAAGGT GCTGCCTTAG GCTGCTTTGT CAAGTCTTGC 
CGTCGAAGCT ATCATGTTCC ATGTGCAAGA GAGATTTCTA GATGCCGTTG GGATTATGAA 
GATTTTCTTC TACTTTGTCC GGCTCATTCT TCTGTCAAAT TCCCGAACGA GAAGTCTGGA 
CATCGCGTTT CCAGAGCCGA GCCCTTACCA AAAATAAATC CTGCTGAGCT ATGTTCTTTG 
GAACAAACAC CAGCTTTTAC AAAAGAGCTT GTTCTCTGTG GATCAGCACT CTCTAAAAGT 
GATAAGAAAC TGATGGAAAG TTTAGCCGTT CGATTTAACG CGACCATATC AAGGTACTGG 
AACCCAAGTG TTACACATGT GATTGCTTCT ACAGATGAAA AAGGAGCTTG CACAAGAACT 
TTGAAGGTTC TTATGGGTAT TCTCAATGGA AAATGGATTA TCAATGCAGC TTGGATGAAA 
GCAAGCCTAA AAGCTTCTCA ACCTGTTGAT GAAGAACCAT TCGAGATTCA AATTGATACT 
CAAGGATGTC AAGATGGACC AAAAACCGCA AGACTCAGAG CAGAAACTAA TAAACCAAAG 
CTCTTTGAGG GCTTGAAGTT CTATTTCTTT GGAGATTTCT ATAAAGGATA CAAGGAAGAC 
CTTCAGAATC TAGTCAAAGT CGCTGGTGGT ACAATCTTGA ACACAGAGGA TGAGCTTGGT 
GCAGAAAGCA GCAACAATGT CAATGACCAA AGATCATCAT CGATTGTGGT TTACAACATT 
GATCCTCCAC ATGGATGTGC CTTAGGTGAA GAAGTCACAA TCATTTGGCA ACGAGCTAAT 
GACGCAGAAG CTTTAGCAAG CCAAACTGGG TCTCGTCTCG TTGGTCACAC ATGGGTCTTG 
GAGTCCATTG CTGGTTACAA GTTACATCCT GTGATTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_973758.2
CDS	74..2215
Translation	MAEFTNMLMNPWVLHLQKLELELKCPLCLKLLNRPVLLPCDHVFCDSCVHKSSQVESGCPVCKSKHPKKGKRDLRFMESVISIYKSLNAAVSVHLPQLQIPNDCNYKNDALNNSNSPKHGESEDSEMTDKDVSKRSGGTDSSSRDGSPLPTSEESDPRPKHQDWTEKQLSDHLLLYEFESEYDAANHTPESYTEQAAKNVRDITASEQPSNAARKRICGDSFIQESSPNPKTQDPTLLRLMESLRSDDPTDYVKAQNHQLPKSHTEQDSKRKRDITASDAMENHLKVPKRENNLMQKSADIDCNGKCSANSDDQLSEKISKALEQTSSNITICGFCQSARVSEATGEMLHYSRGRPVDGDDIFRSNVIHVHSACIEWAPQVYYEGDTVKNLKAELARGMKIKCTKCSLKGAALGCFVKSCRRSYHVPCAREISRCRWDYEDFLLLCPAHSSVKFPNEKSGHRVSRAEPLPKINPAELCSLEQTPAFTKELVLCGSALSKSDKKLMESLAVRFNATISRYWNPSVTHVIASTDEKGACTRTLKVLMGILNGKWIINAAWMKASLKASQPVDEEPFEIQIDTQGCQDGPKTARLRAETNKPKLFEGLKFYFFGDFYKGYKEDLQNLVKVAGGTILNTEDELGAESSNNVNDQRSSSIVVYNIDPPHGCALGEEVTIIWQRANDAEALASQTGSRLVGHTWVLESIAGYKLHPVIG

Target ORF information:

RefSeq Version	NM_202029.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana breast cancer associated RING 1 (BARD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202029.2

ORF Insert Sequence:

ATGGCGGAAT TTACTAACAT GCTAATGAAT CCATGGGTTC TTCATCTCCA GAAGCTCGAG 
CTCGAGCTCA AGTGCCCTCT ATGCTTAAAA TTGCTTAATC GACCGGTGTT GCTTCCTTGC 
GACCACGTCT TCTGCGATTC ATGTGTACAC AAATCGTCGC AAGTTGAATC GGGATGTCCT 
GTCTGCAAAT CTAAACATCC CAAAAAGGGT AAGAGAGATC TTCGATTCAT GGAGAGTGTT 
ATAAGCATAT ACAAGAGCTT GAATGCAGCT GTTAGTGTTC ATCTCCCACA ATTACAGATT 
CCAAATGATT GCAACTACAA GAACGATGCA TTGAACAATT CCAACAGCCC TAAACATGGC 
GAATCTGAAG ATTCTGAGAT GACTGACAAG GATGTGAGCA AGCGCAGTGG TGGTACTGAT 
TCTTCTAGTC GTGATGGTTC TCCTCTTCCG ACCTCAGAAG AAAGTGATCC AAGACCTAAG 
CATCAGGATT GGACTGAAAA GCAGTTGAGT GATCATCTCC TATTATATGA GTTTGAATCA 
GAGTATGATG CTGCCAATCA CACTCCGGAA AGTTATACAG AACAAGCTGC AAAAAATGTG 
AGGGACATCA CTGCTTCTGA GCAGCCTAGC AATGCTGCAA GGAAGCGGAT ATGTGGTGAT 
TCCTTTATCC AGGAAAGCAG TCCGAATCCT AAGACTCAGG ATCCGACTTT GCTGCGGTTG 
ATGGAGAGCC TCCGGTCAGA TGATCCCACT GATTATGTTA AAGCACAGAA CCATCAGTTA 
CCCAAAAGTC ATACAGAACA AGATTCAAAA AGGAAGCGTG ACATCACCGC TTCTGATGCA 
ATGGAAAATC ATCTGAAGGT CCCCAAGAGG GAGAATAATC TGATGCAAAA ATCTGCTGAT 
ATTGACTGCA ATGGTAAATG CTCTGCAAAC TCTGATGATC AGCTAAGTGA GAAAATCTCA 
AAGGCATTAG AACAAACATC ATCCAACATA ACCATTTGTG GTTTCTGCCA ATCCGCTAGG 
GTATCTGAGG CCACTGGAGA AATGCTGCAT TACTCTAGGG GAAGACCGGT GGATGGAGAT 
GACATCTTCC GCTCCAATGT TATCCATGTC CATAGCGCAT GTATCGAATG GGCACCGCAA 
GTTTACTATG AAGGTGACAC AGTGAAGAAC CTGAAAGCAG AACTGGCAAG GGGGATGAAA 
ATCAAGTGCA CTAAATGTAG TCTGAAAGGT GCTGCCTTAG GCTGCTTTGT CAAGTCTTGC 
CGTCGAAGCT ATCATGTTCC ATGTGCAAGA GAGATTTCTA GATGCCGTTG GGATTATGAA 
GATTTTCTTC TACTTTGTCC GGCTCATTCT TCTGTCAAAT TCCCGAACGA GAAGTCTGGA 
CATCGCGTTT CCAGAGCCGA GCCCTTACCA AAAATAAATC CTGCTGAGCT ATGTTCTTTG 
GAACAAACAC CAGCTTTTAC AAAAGAGCTT GTTCTCTGTG GATCAGCACT CTCTAAAAGT 
GATAAGAAAC TGATGGAAAG TTTAGCCGTT CGATTTAACG CGACCATATC AAGGTACTGG 
AACCCAAGTG TTACACATGT GATTGCTTCT ACAGATGAAA AAGGAGCTTG CACAAGAACT 
TTGAAGGTTC TTATGGGTAT TCTCAATGGA AAATGGATTA TCAATGCAGC TTGGATGAAA 
GCAAGCCTAA AAGCTTCTCA ACCTGTTGAT GAAGAACCAT TCGAGATTCA AATTGATACT 
CAAGGATGTC AAGATGGACC AAAAACCGCA AGACTCAGAG CAGAAACTAA TAAACCAAAG 
CTCTTTGAGG GCTTGAAGTT CTATTTCTTT GGAGATTTCT ATAAAGGATA CAAGGAAGAC 
CTTCAGAATC TAGTCAAAGT CGCTGGTGGT ACAATCTTGA ACACAGAGGA TGAGCTTGGT 
GCAGAAAGCA GCAACAATGT CAATGACCAA AGATCATCAT CGATTGTGGT TTACAACATT 
GATCCTCCAC ATGGATGTGC CTTAGGTGAA GAAGTCACAA TCATTTGGCA ACGAGCTAAT 
GACGCAGAAG CTTTAGCAAG CCAAACTGGG TCTCGTCTCG TTGGTCACAC ATGGGTCTTG 
GAGTCCATTG CTGGTTACAA GTTACATCCT GTGATTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BARD1 ( NM_001331456.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318917.1 Arabidopsis thaliana breast cancer associated RING 1 (BARD1), mRNA.

Price & Availability↑

CloneID	OAb32923
Clone ID Related Accession (Same CDS sequence)	NM_001331456.1
Accession Version	NM_001331456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	breast cancer associated RING 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCGGAAT TTACTAACAT GCTAATGAAT CCATGGGTTC TTCATCTCCA GAAGCTCGAG 
CTCGAGCTCA AGTGCCCTCT ATGCTTAAAA TTGCTTAATC GACCGGTGTT GCTTCCTTGC 
GACCACGTCT TCTGCGATTC ATGTGTACAC AAATCGTCGC AAGTTGAATC GGGATGTCCT 
GTCTGCAAAT CTAAACATCC CAAAAAGGCT AGGAGAGATC TTCGATTCAT GGAGAGTGTT 
ATAAGCATAT ACAAGAGCTT GAATGCAGCT GTTAGTGTTC ATCTCCCACA ATTACAGATT 
CCAAATGATT GCAACTACAA GAACGATGCA TTGAACAATT CCAACAGCCC TAAACATGGC 
GAATCTGAAG ATTCTGAGAT GACTGACAAG GATGTGAGCA AGCGCAGTGG TGGTACTGAT 
TCTTCTAGTC GTGATGGTTC TCCTCTTCCG ACCTCAGAAG AAAGTGATCC AAGACCTAAG 
CATCAGGATT GGACTGAAAA GCAGTTGAGT GATCATCTCC TATTATATGA GTTTGAATCA 
GAGTATGATG CTGCCAATCA CACTCCGGAA AGTTATACAG AACAAGCTGC AAAAAATGTG 
AGGGACATCA CTGCTTCTGA GCAGCCTAGC AATGCTGCAA GGAAGCGGAT ATGTGGTGAT 
TCCTTTATCC AGGAAAGCAG TCCGAATCCT AAGACTCAGG ATCCGACTTT GCTGCGGTTG 
ATGGAGAGCC TCCGGTCAGA TGATCCCACT GATTATGTTA AAGCACAGAA CCATCAGTTA 
CCCAAAAGTC ATACAGAACA AGATTCAAAA AGGAAGCGTG ACATCACCGC TTCTGATGCA 
ATGGAAAATC ATCTGAAGGT CCCCAAGAGG GAGAATAATC TGATGCAAAA ATCTGCTGAT 
ATTGACTGCA ATGGTAAATG CTCTGCAAAC TCTGATGATC AGCTAAGTGA GAAAATCTCA 
AAGGCATTAG AACAAACATC ATCCAACATA ACCATTTGTG GTTTCTGCCA ATCCGCTAGG 
GTATCTGAGG CCACTGGAGA AATGCTGCAT TACTCTAGGG GAAGACCGGT GGATGGAGAT 
GACATCTTCC GCTCCAATGT TATCCATGTC CATAGCGCAT GTATCGAATG GGCACCGCAA 
GTTTACTATG AAGGTGACAC AGTGAAGAAC CTGAAAGCAG AACTGGCAAG GGGGATGAAA 
ATCAAGTGCA CTAAATGTAG TCTGAAAGGT GCTGCCTTAG GCTGCTTTGT CAAGTCTTGC 
CGTCGAAGCT ATCATGTTCC ATGTGCAAGA GAGATTTCTA GATGCCGTTG GGATTATGAA 
GATTTTCTTC TACTTTGTCC GGCTCATTCT TCTGTCAAAT TCCCGAACGA GAAGTCTGGA 
CATCGCGTTT CCAGAGCCGA GCCCTTACCA AAAATAAATC CTGCTGAGCT ATGTTCTTTG 
GAACAAACAC CAGCTTTTAC AAAAGAGCTT GTTCTCTGTG GATCAGCACT CTCTAAAAGT 
GATAAGAAAC TGATGGAAAG TTTAGCCGTT CGATTTAACG CGACCATATC AAGGTACTGG 
AACCCAAGTG TTACACATGT GATTGCTTCT ACAGATGAAA AAGGAGCTTG CACAAGAACT 
TTGAAGGTTC TTATGGGTAT TCTCAATGGA AAATGGATTA TCAATGCAGC TTGGATGAAA 
GCAAGCCTAA AAGCTTCTCA ACCTGTTGAT GAAGAACCAT TCGAGATTCA AATTGATACT 
CAAGGATGTC AAGATGGACC AAAAACCGCA AGACTCAGAG CAGAAACTAA TAAACCAAAG 
CTCTTTGAGG GCTTGAAGTT CTATTTCTTT GGAGATTTCT ATAAAGGATA CAAGGAAGAC 
CTTCAGAATC TAGTCAAAGT CGCTGGTGGT ACAATCTTGA ACACAGAGGA TGAGCTTGGT 
GCAGAAAGCA GCAACAATGT CAATGACCAA AGATCATCAT CGATTGTGGT TTACAACATT 
GATCCTCCAC ATGGATGTGC CTTAGGTGAA GAAGTCACAA TCATTTGGCA ACGAGCTAAT 
GACGCAGAAG CTTTAGCAAG CCAAACTGGG TCTCGTCTCG TTGGTCACAC ATGGGTCTTG 
GAGTCCATTG CTGGTTACAA GTTACATCCT GTGATTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318917.1
CDS	118..2259
Translation	MAEFTNMLMNPWVLHLQKLELELKCPLCLKLLNRPVLLPCDHVFCDSCVHKSSQVESGCPVCKSKHPKKARRDLRFMESVISIYKSLNAAVSVHLPQLQIPNDCNYKNDALNNSNSPKHGESEDSEMTDKDVSKRSGGTDSSSRDGSPLPTSEESDPRPKHQDWTEKQLSDHLLLYEFESEYDAANHTPESYTEQAAKNVRDITASEQPSNAARKRICGDSFIQESSPNPKTQDPTLLRLMESLRSDDPTDYVKAQNHQLPKSHTEQDSKRKRDITASDAMENHLKVPKRENNLMQKSADIDCNGKCSANSDDQLSEKISKALEQTSSNITICGFCQSARVSEATGEMLHYSRGRPVDGDDIFRSNVIHVHSACIEWAPQVYYEGDTVKNLKAELARGMKIKCTKCSLKGAALGCFVKSCRRSYHVPCAREISRCRWDYEDFLLLCPAHSSVKFPNEKSGHRVSRAEPLPKINPAELCSLEQTPAFTKELVLCGSALSKSDKKLMESLAVRFNATISRYWNPSVTHVIASTDEKGACTRTLKVLMGILNGKWIINAAWMKASLKASQPVDEEPFEIQIDTQGCQDGPKTARLRAETNKPKLFEGLKFYFFGDFYKGYKEDLQNLVKVAGGTILNTEDELGAESSNNVNDQRSSSIVVYNIDPPHGCALGEEVTIIWQRANDAEALASQTGSRLVGHTWVLESIAGYKLHPVIG

Target ORF information:

RefSeq Version	NM_001331456.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana breast cancer associated RING 1 (BARD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331456.1

ORF Insert Sequence:

ATGGCGGAAT TTACTAACAT GCTAATGAAT CCATGGGTTC TTCATCTCCA GAAGCTCGAG 
CTCGAGCTCA AGTGCCCTCT ATGCTTAAAA TTGCTTAATC GACCGGTGTT GCTTCCTTGC 
GACCACGTCT TCTGCGATTC ATGTGTACAC AAATCGTCGC AAGTTGAATC GGGATGTCCT 
GTCTGCAAAT CTAAACATCC CAAAAAGGCT AGGAGAGATC TTCGATTCAT GGAGAGTGTT 
ATAAGCATAT ACAAGAGCTT GAATGCAGCT GTTAGTGTTC ATCTCCCACA ATTACAGATT 
CCAAATGATT GCAACTACAA GAACGATGCA TTGAACAATT CCAACAGCCC TAAACATGGC 
GAATCTGAAG ATTCTGAGAT GACTGACAAG GATGTGAGCA AGCGCAGTGG TGGTACTGAT 
TCTTCTAGTC GTGATGGTTC TCCTCTTCCG ACCTCAGAAG AAAGTGATCC AAGACCTAAG 
CATCAGGATT GGACTGAAAA GCAGTTGAGT GATCATCTCC TATTATATGA GTTTGAATCA 
GAGTATGATG CTGCCAATCA CACTCCGGAA AGTTATACAG AACAAGCTGC AAAAAATGTG 
AGGGACATCA CTGCTTCTGA GCAGCCTAGC AATGCTGCAA GGAAGCGGAT ATGTGGTGAT 
TCCTTTATCC AGGAAAGCAG TCCGAATCCT AAGACTCAGG ATCCGACTTT GCTGCGGTTG 
ATGGAGAGCC TCCGGTCAGA TGATCCCACT GATTATGTTA AAGCACAGAA CCATCAGTTA 
CCCAAAAGTC ATACAGAACA AGATTCAAAA AGGAAGCGTG ACATCACCGC TTCTGATGCA 
ATGGAAAATC ATCTGAAGGT CCCCAAGAGG GAGAATAATC TGATGCAAAA ATCTGCTGAT 
ATTGACTGCA ATGGTAAATG CTCTGCAAAC TCTGATGATC AGCTAAGTGA GAAAATCTCA 
AAGGCATTAG AACAAACATC ATCCAACATA ACCATTTGTG GTTTCTGCCA ATCCGCTAGG 
GTATCTGAGG CCACTGGAGA AATGCTGCAT TACTCTAGGG GAAGACCGGT GGATGGAGAT 
GACATCTTCC GCTCCAATGT TATCCATGTC CATAGCGCAT GTATCGAATG GGCACCGCAA 
GTTTACTATG AAGGTGACAC AGTGAAGAAC CTGAAAGCAG AACTGGCAAG GGGGATGAAA 
ATCAAGTGCA CTAAATGTAG TCTGAAAGGT GCTGCCTTAG GCTGCTTTGT CAAGTCTTGC 
CGTCGAAGCT ATCATGTTCC ATGTGCAAGA GAGATTTCTA GATGCCGTTG GGATTATGAA 
GATTTTCTTC TACTTTGTCC GGCTCATTCT TCTGTCAAAT TCCCGAACGA GAAGTCTGGA 
CATCGCGTTT CCAGAGCCGA GCCCTTACCA AAAATAAATC CTGCTGAGCT ATGTTCTTTG 
GAACAAACAC CAGCTTTTAC AAAAGAGCTT GTTCTCTGTG GATCAGCACT CTCTAAAAGT 
GATAAGAAAC TGATGGAAAG TTTAGCCGTT CGATTTAACG CGACCATATC AAGGTACTGG 
AACCCAAGTG TTACACATGT GATTGCTTCT ACAGATGAAA AAGGAGCTTG CACAAGAACT 
TTGAAGGTTC TTATGGGTAT TCTCAATGGA AAATGGATTA TCAATGCAGC TTGGATGAAA 
GCAAGCCTAA AAGCTTCTCA ACCTGTTGAT GAAGAACCAT TCGAGATTCA AATTGATACT 
CAAGGATGTC AAGATGGACC AAAAACCGCA AGACTCAGAG CAGAAACTAA TAAACCAAAG 
CTCTTTGAGG GCTTGAAGTT CTATTTCTTT GGAGATTTCT ATAAAGGATA CAAGGAAGAC 
CTTCAGAATC TAGTCAAAGT CGCTGGTGGT ACAATCTTGA ACACAGAGGA TGAGCTTGGT 
GCAGAAAGCA GCAACAATGT CAATGACCAA AGATCATCAT CGATTGTGGT TTACAACATT 
GATCCTCCAC ATGGATGTGC CTTAGGTGAA GAAGTCACAA TCATTTGGCA ACGAGCTAAT 
GACGCAGAAG CTTTAGCAAG CCAAACTGGG TCTCGTCTCG TTGGTCACAC ATGGGTCTTG 
GAGTCCATTG CTGGTTACAA GTTACATCCT GTGATTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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BARD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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