eIFiso4G1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIFiso4G1
Entrez Gene ID	835897
Full Name	MIF4G domain-containing protein / MA3 domain-containing protein
Synonyms	MTI20.13,MTI20_13,eukaryotic translation Initiation Factor isoform 4G1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_125172.2	NP_200595.2	MIF4G domain-containing protein / MA3 domain-containing protein
NM_180876.2	NP_851207.1	MIF4G domain-containing protein / MA3 domain-containing protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0611500	NP_001047407.1
Arabidopsis thaliana (thale cress)	eIFiso4G1	NP_851207.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study on a mutant of eIFiso4G1 uncovered multifaceted interactions between the cytosolic and plastidic compartments in seed lipid biosynthesis that impact major seed oil traits.
Title: Effects of eIFiso4G1 mutation on seed oil biosynthesis.

Data (including phylogenetic analysis) suggest that eukaryotic translation initiation factors eIF4G1/eIF(iso)4G1 and eIF4G2/eIF(iso)4G2 exhibit differences in ability to promote translation initiation. [eIF4G1]
Title: Eukaryotic Initiation Factor eIFiso4G1 and eIFiso4G2 Are Isoforms Exhibiting Distinct Functional Differences in Supporting Translation in Arabidopsis.

Single and double knockout deletions of eIFis4G1 (i4g1) and eIFiso4G2 (i4g2) genes in A. thaliana reduce the amounts of chlorophyll a and b, suggesting a role in the expression of chloroplast proteins. [i4g1] [eIFiso4G1]
Title: Deletion of the eIFiso4G subunit of the Arabidopsis eIFiso4F translation initiation complex impairs health and viability.

The following eIFiso4G1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIFiso4G1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb21724	NM_125172.2 Latest version!	Arabidopsis thaliana MIF4G domain-containing protein / MA3 domain-containing protein (eIFiso4G1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb21725	NM_180876.2 Latest version!	Arabidopsis thaliana MIF4G domain-containing protein / MA3 domain-containing protein (eIFiso4G1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIFiso4G1 ( NM_125172.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200595.2 Arabidopsis thaliana MIF4G domain-containing protein / MA3 domain-containing protein (eIFiso4G1), mRNA.

Price & Availability↑

CloneID	OAb21724
Clone ID Related Accession (Same CDS sequence)	NM_125172.2
Accession Version	NM_125172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MIF4G domain-containing protein / MA3 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On May 13, 2003 this sequence version replaced NM_125172.1.

ATGCAGCAAG GCGATCAAAC GGTTCTGAGC TTGAGACCTG GTGGCGGTCG AGGAAACAGA 
CTATTTGGTT CCTCTTCTTC TTCTTCGTCT CTCTCTTTTG GCTCTCTTTC ATCTTCGGAT 
CTACCTCTCC TTCGTCCTCA TGGTGGTGCT CCTGCTTCCT CTTTTCCCTT CAAGGGTGGA 
GACTCGAGGT TCGATGGCCG TGAACGTGTG AAGTACACCC GTGAGCAGCT CCTGGAGCTT 
AAAGAGACCA CTCAACTCTC TGATGAAATT CTGAGAGTCC AACGGGAAAC TGCAGCTGAA 
CTGTTTGGTG AGGAAGGGAC TTGGGCACGT GGAGAAAGTG TCGTTCCCGT TCAATCCGCA 
AGTCGCTTCT CTGAGCCAGA CTCTCGTGAT TGGCGTAGCC GTTCTACTCA ACCTCCACCC 
TCTGGGGAGG AAAGGTCGTG GGATAATCTT CGTGAAGCTA AAGATTCCAG GTATGTTGAA 
GCAAGTCAAT ATAATCGTCA AGACCAACCT AATTCCCAGT TTTCAAGGGC AAACATATCA 
TCCAACCAAG GGGGTGGACC AGCTCCTGTC CTCGTCAAAG CCGAAGTTCC TTGGTCAGCT 
AGAAGAGGAA ACCTTTCTGA AAATGATCGT GTTCTGAAGA CTGTGAAAGG GATACTGAAC 
AAGCTAACCC CTGAAAAGTA TGACCTTCTC AAGGGCCAAC TTATTGAATC TGGCATCACA 
TCTGCTGATA TCCTGAAGGG TGTGATTACC CTTATTTTTG ACAAAGCTGT CCTTGAGCCG 
ACCTTTTGCC CTATGTATGC AAAATTGTGC TCTGATATCA ACGACCAGCT GCCCACCTTC 
CCACCTGCAG AGCCTGGGGA TAAGGAGATT ACATTTAAGA GGGTTCTACT GAATATCTGT 
CAAGAGGCAT TTGAGGGCGC TTCTCAGTTG AGGGAGGAGC TTAGACAGAT GAGTGCTCCA 
GATCAGGAGG CTGAGCGGAA TGACAAAGAA AAATTGTTGA AGCTCAAGAC TCTAGGAAAT 
ATTCGTTTGA TTGGTGAGCT TTTGAAGCAG AAGATGGTCC CAGAAAAGAT TGTTCATCAC 
ATTGTTCAGG AGCTTCTGGG GGCCGATGAG AAAGTTTGTC CAGCCGAGGA AAATGTCGAA 
GCTATTTGTC ATTTCTTCAA AACTATTGGC AAACAACTTG ACGGAAACGT GAAGTCAAAA 
CGGATTAATG ATGTATATTT CAAACGCCTA CAGGCTCTGT CAAAGAATCC TCAGTTGGAA 
CTGCGTCTGA GATTCATGGT TCAGAATATC ATCGACATGC GCTCCAATGG CTGGGTTCCA 
AGGCGGGAAG AGATGAAAGC AAGAACTATA ACCGAAATAC ATACTGAGGC AGAGAAGAAT 
CTTGGTCTCC GACCAGGTGC CACTGCAAAT ATGAGAAGGG GTATGGTTTC TAGTGGTGGG 
CCTGTGAGCC CTGGCCCTGT CTACCCTGGT GGGCGACCTG GTGCGGGTGG CTTGATGCCT 
GGTATGCCTG GGACGCGAAG GATGCCTGGG ATGCCCGGAG TGGACAATGA TAACTGGGAG 
GTACCCCGGA CAAGATCAAT GTCTAGACGC GATGGGCCAG GACCTCTACA TTCTCCAGCA 
GTGAGCAAGT CGGCCTCAAT GAACACGAGG CTCTTACCTC AAGGCAGCAG TGGCATTATG 
AGTGGCAAGA CAAGCGCACT GTTGCAGGGA AGTGGTTCAG TGTCGCGTCC AGTTACCGTG 
TCAGCAGAAC GGCCAGCACA GTCAGTTGCA CCTCTGACCG TACCAGTTCC GGTGGAGAAA 
CCACAGCCTA GTGGTCCCAA GTTGAGCGAA GAAGTGCTCC AAAGAAAAAC AAAGTCGCTT 
CTCGAGGAAT ATTTCAATGT CCGACTACTT GGTGAAGCTC TGCAGTGTGT AGAGGAGCTT 
GGTTTGCCGT CATATCACCC AGAGTTTGTG AAGGAAGCAA TATCCCTGTC TCTTGAGAAA 
AGCCCGCCTG TCGTTGAGCC AATTGCTACA CTCTTGGAGT ATTTGTTATC AAAGAAGGTA 
GTCGCACCCA AGGATCTTGA AACTGGGTTC TTGCTCTACG GTGCTATGTT GGACGACATT 
GGAATTGATC TTCCTAAGGC ACCAAACAAT TTTGGTGAGA TTGTGGGAAA GCTGATTCTG 
GCTGGTGGTG TGGATTTCAA ATTGGTTAGA GAAATTATAG GGAAGATGGA AGACGATAGG 
TTCCAGAAGA TGGTGGTCGA TGCAGCTGTA CGCATTGTGG AGTCAAGCGA GCAGGGGAAG 
TCCTTGTTGG CCTCACAGGC TGCAGACATA GAGGCTTGTC GGAATCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200595.2
CDS	258..2588
Translation	MQQGDQTVLSLRPGGGRGNRLFGSSSSSSSLSFGSLSSSDLPLLRPHGGAPASSFPFKGGDSRFDGRERVKYTREQLLELKETTQLSDEILRVQRETAAELFGEEGTWARGESVVPVQSASRFSEPDSRDWRSRSTQPPPSGEERSWDNLREAKDSRYVEASQYNRQDQPNSQFSRANISSNQGGGPAPVLVKAEVPWSARRGNLSENDRVLKTVKGILNKLTPEKYDLLKGQLIESGITSADILKGVITLIFDKAVLEPTFCPMYAKLCSDINDQLPTFPPAEPGDKEITFKRVLLNICQEAFEGASQLREELRQMSAPDQEAERNDKEKLLKLKTLGNIRLIGELLKQKMVPEKIVHHIVQELLGADEKVCPAEENVEAICHFFKTIGKQLDGNVKSKRINDVYFKRLQALSKNPQLELRLRFMVQNIIDMRSNGWVPRREEMKARTITEIHTEAEKNLGLRPGATANMRRGMVSSGGPVSPGPVYPGGRPGAGGLMPGMPGTRRMPGMPGVDNDNWEVPRTRSMSRRDGPGPLHSPAVSKSASMNTRLLPQGSSGIMSGKTSALLQGSGSVSRPVTVSAERPAQSVAPLTVPVPVEKPQPSGPKLSEEVLQRKTKSLLEEYFNVRLLGEALQCVEELGLPSYHPEFVKEAISLSLEKSPPVVEPIATLLEYLLSKKVVAPKDLETGFLLYGAMLDDIGIDLPKAPNNFGEIVGKLILAGGVDFKLVREIIGKMEDDRFQKMVVDAAVRIVESSEQGKSLLASQAADIEACRNL

Target ORF information:

RefSeq Version	NM_125172.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MIF4G domain-containing protein / MA3 domain-containing protein (eIFiso4G1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125172.2

ORF Insert Sequence:

ATGCAGCAAG GCGATCAAAC GGTTCTGAGC TTGAGACCTG GTGGCGGTCG AGGAAACAGA 
CTATTTGGTT CCTCTTCTTC TTCTTCGTCT CTCTCTTTTG GCTCTCTTTC ATCTTCGGAT 
CTACCTCTCC TTCGTCCTCA TGGTGGTGCT CCTGCTTCCT CTTTTCCCTT CAAGGGTGGA 
GACTCGAGGT TCGATGGCCG TGAACGTGTG AAGTACACCC GTGAGCAGCT CCTGGAGCTT 
AAAGAGACCA CTCAACTCTC TGATGAAATT CTGAGAGTCC AACGGGAAAC TGCAGCTGAA 
CTGTTTGGTG AGGAAGGGAC TTGGGCACGT GGAGAAAGTG TCGTTCCCGT TCAATCCGCA 
AGTCGCTTCT CTGAGCCAGA CTCTCGTGAT TGGCGTAGCC GTTCTACTCA ACCTCCACCC 
TCTGGGGAGG AAAGGTCGTG GGATAATCTT CGTGAAGCTA AAGATTCCAG GTATGTTGAA 
GCAAGTCAAT ATAATCGTCA AGACCAACCT AATTCCCAGT TTTCAAGGGC AAACATATCA 
TCCAACCAAG GGGGTGGACC AGCTCCTGTC CTCGTCAAAG CCGAAGTTCC TTGGTCAGCT 
AGAAGAGGAA ACCTTTCTGA AAATGATCGT GTTCTGAAGA CTGTGAAAGG GATACTGAAC 
AAGCTAACCC CTGAAAAGTA TGACCTTCTC AAGGGCCAAC TTATTGAATC TGGCATCACA 
TCTGCTGATA TCCTGAAGGG TGTGATTACC CTTATTTTTG ACAAAGCTGT CCTTGAGCCG 
ACCTTTTGCC CTATGTATGC AAAATTGTGC TCTGATATCA ACGACCAGCT GCCCACCTTC 
CCACCTGCAG AGCCTGGGGA TAAGGAGATT ACATTTAAGA GGGTTCTACT GAATATCTGT 
CAAGAGGCAT TTGAGGGCGC TTCTCAGTTG AGGGAGGAGC TTAGACAGAT GAGTGCTCCA 
GATCAGGAGG CTGAGCGGAA TGACAAAGAA AAATTGTTGA AGCTCAAGAC TCTAGGAAAT 
ATTCGTTTGA TTGGTGAGCT TTTGAAGCAG AAGATGGTCC CAGAAAAGAT TGTTCATCAC 
ATTGTTCAGG AGCTTCTGGG GGCCGATGAG AAAGTTTGTC CAGCCGAGGA AAATGTCGAA 
GCTATTTGTC ATTTCTTCAA AACTATTGGC AAACAACTTG ACGGAAACGT GAAGTCAAAA 
CGGATTAATG ATGTATATTT CAAACGCCTA CAGGCTCTGT CAAAGAATCC TCAGTTGGAA 
CTGCGTCTGA GATTCATGGT TCAGAATATC ATCGACATGC GCTCCAATGG CTGGGTTCCA 
AGGCGGGAAG AGATGAAAGC AAGAACTATA ACCGAAATAC ATACTGAGGC AGAGAAGAAT 
CTTGGTCTCC GACCAGGTGC CACTGCAAAT ATGAGAAGGG GTATGGTTTC TAGTGGTGGG 
CCTGTGAGCC CTGGCCCTGT CTACCCTGGT GGGCGACCTG GTGCGGGTGG CTTGATGCCT 
GGTATGCCTG GGACGCGAAG GATGCCTGGG ATGCCCGGAG TGGACAATGA TAACTGGGAG 
GTACCCCGGA CAAGATCAAT GTCTAGACGC GATGGGCCAG GACCTCTACA TTCTCCAGCA 
GTGAGCAAGT CGGCCTCAAT GAACACGAGG CTCTTACCTC AAGGCAGCAG TGGCATTATG 
AGTGGCAAGA CAAGCGCACT GTTGCAGGGA AGTGGTTCAG TGTCGCGTCC AGTTACCGTG 
TCAGCAGAAC GGCCAGCACA GTCAGTTGCA CCTCTGACCG TACCAGTTCC GGTGGAGAAA 
CCACAGCCTA GTGGTCCCAA GTTGAGCGAA GAAGTGCTCC AAAGAAAAAC AAAGTCGCTT 
CTCGAGGAAT ATTTCAATGT CCGACTACTT GGTGAAGCTC TGCAGTGTGT AGAGGAGCTT 
GGTTTGCCGT CATATCACCC AGAGTTTGTG AAGGAAGCAA TATCCCTGTC TCTTGAGAAA 
AGCCCGCCTG TCGTTGAGCC AATTGCTACA CTCTTGGAGT ATTTGTTATC AAAGAAGGTA 
GTCGCACCCA AGGATCTTGA AACTGGGTTC TTGCTCTACG GTGCTATGTT GGACGACATT 
GGAATTGATC TTCCTAAGGC ACCAAACAAT TTTGGTGAGA TTGTGGGAAA GCTGATTCTG 
GCTGGTGGTG TGGATTTCAA ATTGGTTAGA GAAATTATAG GGAAGATGGA AGACGATAGG 
TTCCAGAAGA TGGTGGTCGA TGCAGCTGTA CGCATTGTGG AGTCAAGCGA GCAGGGGAAG 
TCCTTGTTGG CCTCACAGGC TGCAGACATA GAGGCTTGTC GGAATCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIFiso4G1 ( NM_180876.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851207.1 Arabidopsis thaliana MIF4G domain-containing protein / MA3 domain-containing protein (eIFiso4G1), mRNA.

Price & Availability↑

CloneID	OAb21725
Clone ID Related Accession (Same CDS sequence)	NM_180876.2
Accession Version	NM_180876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MIF4G domain-containing protein / MA3 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_180876.1.

ATGCAGCAAG GCGATCAAAC GGTTCTGAGC TTGAGACCTG GTGGCGGTCG AGGAAACAGA 
CTATTTGGTT CCTCTTCTTC TTCTTCGTCT CTCTCTTTTG GCTCTCTTTC ATCTTCGGAT 
CTACCTCTCC TTCGTCCTCA TGGTGGTGCT CCTGCTTCCT CTTTTCCCTT CAAGGGTGGA 
GACTCGAGGT TCGATGGCCG TGAACGTGTG AAGTACACCC GTGAGCAGCT CCTGGAGCTT 
AAAGAGACCA CTCAACTCTC TGATGAAATT CTGAGAGTCC AACGGGAAAC TGCAGCTGAA 
CTGTTTGGTG AGGAAGGGAC TTGGGCACGT GGAGAAAGTG TCGTAAGCAA TTTGGTTCCC 
GTTCAATCCG CAAGTCGCTT CTCTGAGCCA GACTCTCGTG ATTGGCGTAG CCGTTCTACT 
CAACCTCCAC CCTCTGGGGA GGAAAGGTCG TGGGATAATC TTCGTGAAGC TAAAGATTCC 
AGGTATGTTG AAGCAAGTCA ATATAATCGT CAAGACCAAC CTAATTCCCA GTTTTCAAGG 
GCAAACATAT CATCCAACCA AGGGGGTGGA CCAGCTCCTG TCCTCGTCAA AGCCGAAGTT 
CCTTGGTCAG CTAGAAGAGG AAACCTTTCT GAAAATGATC GTGTTCTGAA GACTGTGAAA 
GGGATACTGA ACAAGCTAAC CCCTGAAAAG TATGACCTTC TCAAGGGCCA ACTTATTGAA 
TCTGGCATCA CATCTGCTGA TATCCTGAAG GGTGTGATTA CCCTTATTTT TGACAAAGCT 
GTCCTTGAGC CGACCTTTTG CCCTATGTAT GCAAAATTGT GCTCTGATAT CAACGACCAG 
CTGCCCACCT TCCCACCTGC AGAGCCTGGG GATAAGGAGA TTACATTTAA GAGGGTTCTA 
CTGAATATCT GTCAAGAGGC ATTTGAGGGC GCTTCTCAGT TGAGGGAGGA GCTTAGACAG 
ATGAGTGCTC CAGATCAGGA GGCTGAGCGG AATGACAAAG AAAAATTGTT GAAGCTCAAG 
ACTCTAGGAA ATATTCGTTT GATTGGTGAG CTTTTGAAGC AGAAGATGGT CCCAGAAAAG 
ATTGTTCATC ACATTGTTCA GGAGCTTCTG GGGGCCGATG AGAAAGTTTG TCCAGCCGAG 
GAAAATGTCG AAGCTATTTG TCATTTCTTC AAAACTATTG GCAAACAACT TGACGGAAAC 
GTGAAGTCAA AACGGATTAA TGATGTATAT TTCAAACGCC TACAGGCTCT GTCAAAGAAT 
CCTCAGTTGG AACTGCGTCT GAGATTCATG GTTCAGAATA TCATCGACAT GCGCTCCAAT 
GGCTGGGTTC CAAGGCGGGA AGAGATGAAA GCAAGAACTA TAACCGAAAT ACATACTGAG 
GCAGAGAAGA ATCTTGGTCT CCGACCAGGT GCCACTGCAA ATATGAGAAG GGGTATGGTT 
TCTAGTGGTG GGCCTGTGAG CCCTGGCCCT GTCTACCCTG GTGGGCGACC TGGTGCGGGT 
GGCTTGATGC CTGGTATGCC TGGGACGCGA AGGATGCCTG GGATGCCCGG AGTGGACAAT 
GATAACTGGG AGGTACCCCG GACAAGATCA ATGTCTAGAC GCGATGGGCC AGGACCTCTA 
CATTCTCCAG CAGTGAGCAA GTCGGCCTCA ATGAACACGA GGCTCTTACC TCAAGGCAGC 
AGTGGCATTA TGAGTGGCAA GACAAGCGCA CTGTTGCAGG GAAGTGGTTC AGTGTCGCGT 
CCAGTTACCG TGTCAGCAGA ACGGCCAGCA CAGTCAGTTG CACCTCTGAC CGTACCAGTT 
CCGGTGGAGA AACCACAGCC TAGTGGTCCC AAGTTGAGCG AAGAAGTGCT CCAAAGAAAA 
ACAAAGTCGC TTCTCGAGGA ATATTTCAAT GTCCGACTAC TTGGTGAAGC TCTGCAGTGT 
GTAGAGGAGC TTGGTTTGCC GTCATATCAC CCAGAGTTTG TGAAGGAAGC AATATCCCTG 
TCTCTTGAGA AAAGCCCGCC TGTCGTTGAG CCAATTGCTA CACTCTTGGA GTATTTGTTA 
TCAAAGAAGG TAGTCGCACC CAAGGATCTT GAAACTGGGT TCTTGCTCTA CGGTGCTATG 
TTGGACGACA TTGGAATTGA TCTTCCTAAG GCACCAAACA ATTTTGGTGA GATTGTGGGA 
AAGCTGATTC TGGCTGGTGG TGTGGATTTC AAATTGGTTA GAGAAATTAT AGGGAAGATG 
GAAGACGATA GGTTCCAGAA GATGGTGGTC GATGCAGCTG TACGCATTGT GGAGTCAAGC 
GAGCAGGGGA AGTCCTTGTT GGCCTCACAG GCTGCAGACA TAGAGGCTTG TCGGAATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851207.1
CDS	256..2598
Translation	MQQGDQTVLSLRPGGGRGNRLFGSSSSSSSLSFGSLSSSDLPLLRPHGGAPASSFPFKGGDSRFDGRERVKYTREQLLELKETTQLSDEILRVQRETAAELFGEEGTWARGESVVSNLVPVQSASRFSEPDSRDWRSRSTQPPPSGEERSWDNLREAKDSRYVEASQYNRQDQPNSQFSRANISSNQGGGPAPVLVKAEVPWSARRGNLSENDRVLKTVKGILNKLTPEKYDLLKGQLIESGITSADILKGVITLIFDKAVLEPTFCPMYAKLCSDINDQLPTFPPAEPGDKEITFKRVLLNICQEAFEGASQLREELRQMSAPDQEAERNDKEKLLKLKTLGNIRLIGELLKQKMVPEKIVHHIVQELLGADEKVCPAEENVEAICHFFKTIGKQLDGNVKSKRINDVYFKRLQALSKNPQLELRLRFMVQNIIDMRSNGWVPRREEMKARTITEIHTEAEKNLGLRPGATANMRRGMVSSGGPVSPGPVYPGGRPGAGGLMPGMPGTRRMPGMPGVDNDNWEVPRTRSMSRRDGPGPLHSPAVSKSASMNTRLLPQGSSGIMSGKTSALLQGSGSVSRPVTVSAERPAQSVAPLTVPVPVEKPQPSGPKLSEEVLQRKTKSLLEEYFNVRLLGEALQCVEELGLPSYHPEFVKEAISLSLEKSPPVVEPIATLLEYLLSKKVVAPKDLETGFLLYGAMLDDIGIDLPKAPNNFGEIVGKLILAGGVDFKLVREIIGKMEDDRFQKMVVDAAVRIVESSEQGKSLLASQAADIEACRNL

Target ORF information:

RefSeq Version	NM_180876.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MIF4G domain-containing protein / MA3 domain-containing protein (eIFiso4G1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180876.2

ORF Insert Sequence:

ATGCAGCAAG GCGATCAAAC GGTTCTGAGC TTGAGACCTG GTGGCGGTCG AGGAAACAGA 
CTATTTGGTT CCTCTTCTTC TTCTTCGTCT CTCTCTTTTG GCTCTCTTTC ATCTTCGGAT 
CTACCTCTCC TTCGTCCTCA TGGTGGTGCT CCTGCTTCCT CTTTTCCCTT CAAGGGTGGA 
GACTCGAGGT TCGATGGCCG TGAACGTGTG AAGTACACCC GTGAGCAGCT CCTGGAGCTT 
AAAGAGACCA CTCAACTCTC TGATGAAATT CTGAGAGTCC AACGGGAAAC TGCAGCTGAA 
CTGTTTGGTG AGGAAGGGAC TTGGGCACGT GGAGAAAGTG TCGTAAGCAA TTTGGTTCCC 
GTTCAATCCG CAAGTCGCTT CTCTGAGCCA GACTCTCGTG ATTGGCGTAG CCGTTCTACT 
CAACCTCCAC CCTCTGGGGA GGAAAGGTCG TGGGATAATC TTCGTGAAGC TAAAGATTCC 
AGGTATGTTG AAGCAAGTCA ATATAATCGT CAAGACCAAC CTAATTCCCA GTTTTCAAGG 
GCAAACATAT CATCCAACCA AGGGGGTGGA CCAGCTCCTG TCCTCGTCAA AGCCGAAGTT 
CCTTGGTCAG CTAGAAGAGG AAACCTTTCT GAAAATGATC GTGTTCTGAA GACTGTGAAA 
GGGATACTGA ACAAGCTAAC CCCTGAAAAG TATGACCTTC TCAAGGGCCA ACTTATTGAA 
TCTGGCATCA CATCTGCTGA TATCCTGAAG GGTGTGATTA CCCTTATTTT TGACAAAGCT 
GTCCTTGAGC CGACCTTTTG CCCTATGTAT GCAAAATTGT GCTCTGATAT CAACGACCAG 
CTGCCCACCT TCCCACCTGC AGAGCCTGGG GATAAGGAGA TTACATTTAA GAGGGTTCTA 
CTGAATATCT GTCAAGAGGC ATTTGAGGGC GCTTCTCAGT TGAGGGAGGA GCTTAGACAG 
ATGAGTGCTC CAGATCAGGA GGCTGAGCGG AATGACAAAG AAAAATTGTT GAAGCTCAAG 
ACTCTAGGAA ATATTCGTTT GATTGGTGAG CTTTTGAAGC AGAAGATGGT CCCAGAAAAG 
ATTGTTCATC ACATTGTTCA GGAGCTTCTG GGGGCCGATG AGAAAGTTTG TCCAGCCGAG 
GAAAATGTCG AAGCTATTTG TCATTTCTTC AAAACTATTG GCAAACAACT TGACGGAAAC 
GTGAAGTCAA AACGGATTAA TGATGTATAT TTCAAACGCC TACAGGCTCT GTCAAAGAAT 
CCTCAGTTGG AACTGCGTCT GAGATTCATG GTTCAGAATA TCATCGACAT GCGCTCCAAT 
GGCTGGGTTC CAAGGCGGGA AGAGATGAAA GCAAGAACTA TAACCGAAAT ACATACTGAG 
GCAGAGAAGA ATCTTGGTCT CCGACCAGGT GCCACTGCAA ATATGAGAAG GGGTATGGTT 
TCTAGTGGTG GGCCTGTGAG CCCTGGCCCT GTCTACCCTG GTGGGCGACC TGGTGCGGGT 
GGCTTGATGC CTGGTATGCC TGGGACGCGA AGGATGCCTG GGATGCCCGG AGTGGACAAT 
GATAACTGGG AGGTACCCCG GACAAGATCA ATGTCTAGAC GCGATGGGCC AGGACCTCTA 
CATTCTCCAG CAGTGAGCAA GTCGGCCTCA ATGAACACGA GGCTCTTACC TCAAGGCAGC 
AGTGGCATTA TGAGTGGCAA GACAAGCGCA CTGTTGCAGG GAAGTGGTTC AGTGTCGCGT 
CCAGTTACCG TGTCAGCAGA ACGGCCAGCA CAGTCAGTTG CACCTCTGAC CGTACCAGTT 
CCGGTGGAGA AACCACAGCC TAGTGGTCCC AAGTTGAGCG AAGAAGTGCT CCAAAGAAAA 
ACAAAGTCGC TTCTCGAGGA ATATTTCAAT GTCCGACTAC TTGGTGAAGC TCTGCAGTGT 
GTAGAGGAGC TTGGTTTGCC GTCATATCAC CCAGAGTTTG TGAAGGAAGC AATATCCCTG 
TCTCTTGAGA AAAGCCCGCC TGTCGTTGAG CCAATTGCTA CACTCTTGGA GTATTTGTTA 
TCAAAGAAGG TAGTCGCACC CAAGGATCTT GAAACTGGGT TCTTGCTCTA CGGTGCTATG 
TTGGACGACA TTGGAATTGA TCTTCCTAAG GCACCAAACA ATTTTGGTGA GATTGTGGGA 
AAGCTGATTC TGGCTGGTGG TGTGGATTTC AAATTGGTTA GAGAAATTAT AGGGAAGATG 
GAAGACGATA GGTTCCAGAA GATGGTGGTC GATGCAGCTG TACGCATTGT GGAGTCAAGC 
GAGCAGGGGA AGTCCTTGTT GGCCTCACAG GCTGCAGACA TAGAGGCTTG TCGGAATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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eIFiso4G1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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