PNG1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNG1
Entrez Gene ID	835019
Full Name	peptide-N-glycanase 1
Synonyms	AtPNG1,K6M13.12,K6M13_12,peptide-N-glycanase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_124335.3	NP_199768.1	peptide-N-glycanase 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os07g0497400	NP_001059707.1
Arabidopsis thaliana (thale cress)	PNG1	NP_199768.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The plant PNGase facilitates ERAD through its deglycosylation activity, while the catalytic mutant of AtPNG1 impair glycoprotein ERAD by binding to N-glycans on the ERAD substrates.
Title: A plant peptide: N-glycanase orthologue facilitates glycoprotein ER-associated degradation in yeast.

AtPng1p, the first plant transglutaminase sequenced shows undetectable sequence homology to the animal enzymes, except for the catalytic triad. It is, however, endowed with a calcium-dependent activity that allowed us to build a three-dimensional model.
Title: Plant and animal transglutaminases: do similar functions imply similar structures?

the AtPng1p gene product acts as a transglutaminase; the first reported plant protein, characterized at molecular level showing TGase activity. [AtPng1p]
Title: AtPng1p. The first plant transglutaminase.

The following PNG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb21025	NM_124335.3 Latest version!	Arabidopsis thaliana peptide-N-glycanase 1 (PNG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNG1 ( NM_124335.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199768.1 Arabidopsis thaliana peptide-N-glycanase 1 (PNG1), mRNA.

Price & Availability↑

CloneID	OAb21025
Clone ID Related Accession (Same CDS sequence)	NM_124335.3
Accession Version	NM_124335.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	peptide-N-glycanase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124335.2.

ATGGTTGCTC GGAAATTTGT CGTCCGCCAT GAAGATTCGA GCTTCGATGT CGACTACAAC 
ACTGAAGATG GTCTTGAGGT TTTACGATTT CTAATCTTCT CCCTCACTTT AGTTCCTCCT 
GAAGAACAAA AGATCGTTGC GGAAGATGAT AATCGATTGG TGTCTGATGA GTCTGATCTC 
GCTTCTTTAT CTGAGAGACT TCGATTAGTT TCAGTTGGAG AAGATTCTGT AGAGAACTCA 
GATGCGGAAA TGTTGAAATC AGATGAAGAA TTGGCTCGAA TGTTGCAGGC AGAAGAAGAT 
GCTATTATGT TTCAACAGTT TGTCGCTGCA CGAGATAACG GCGAATTCGA AGGGAGAATT 
AGGCCTTATG TTAGTCAAGT TCTCATGTAT GAAGATCCAG TCCGCCAAGA TGCTGCTCGA 
AAGACTGTTC CTAAAGACGA GCTTGAGGAG AAAGCATTGG TTTCTCTGGC CAAGGAAGGG 
AATTTTGAGC CATCGAAAGA GGAAAGAGAC TACGCTTTCC TGCTTCAGCT TCTTTTCTGG 
TTCAAAAAAT CCTTCAGATG GGTCAATGAA CCTCCTTGTG ACTTTTGTGG TAACAAAACC 
ATAGGCCAGG GGATGGGAAA CCCACTTACC TCGGAGCTTG CTTATGGAGC AAATCGAGTT 
GAAATATATC GCTGTACCAT GTGTCCAACA ACTACTCGAT TCCCTCGGTA CAACGATCCG 
CTAAAGCTCG TAGAAACAAA GAAAGGTCGT TGCGGAGAGT GGGCGAACTG CTTTACGCTA 
TACTGCCGCA CGTTTGGCTA TGATTCTCGT TTGATAATGG ACTTCACAGA TCACGTGTGG 
ACAGAATGCT ACTCTCACTC GTTAAAGAGG TGGATCCATC TTGACCCTTG TGAAGGAGTG 
TATGATAAAC CCATGTTATA TGAGAAAGGA TGGAATAAAA AATTGAATTA TGTCATTGCG 
ATTTCTAAGG ACGGGGTTTG TGATGTTACC AAGCGATATA CAAAGAAATG GCATGAGGTT 
TTATCTAGAA GAACTCTCAC AACTGAATCA TCTTTGCAAG ATGGTCTTCG AACCCTGACA 
AGGGAACGAC GACGTAGCTT AATGTTTGAA TCTCTATCTA AGCTTGAATT ACGAGACAGA 
AATGAGCAGG AAGAACTAGA GAGAAATCTG CATTCCGCAG ATAATGCCTC AGTTTCTTTA 
CCCGGAAGGC AAAGTGGGGA CAGGGAATGG CGTATTATGA GATCAGAATT TGGTTCAGAC 
GAAAACTCAT CTGTCAGCTC TTCCTCTTGC CCAGTTCGCA AATGCGTTGA TGACCATGTG 
ACCAACATTT ATGACTCGTT TCTACCTATC CTTACTCAGT TCGTTGAGGA CGGCTTACCT 
GTAGCAAGAA CAAATGAGGT ACTTAAGATG ATCAAGCAAG TCCTAGTGGA TCTGAAGAAT 
GCGCCTTACA AAACAAGGAA GGCTCGATTG ACTTTAGATT CAGATAACTC AAGTTCATTC 
CCGGAGCAGT TTCTTCCCGC ACTAGGCGAT TTGCTTCTCG CTCTTTCTTT AAAGAGCGAG 
AGAGACACAA ACGGTAAAAG TGTCACAATA TCCGTAGACG GGAAACTCAC TAAAACTGCT 
ATAGCATTGC CAGTTGCATT GGACGCGTTA AGGGAACTTG TTGCTGACCT CAGCAAATAC 
CAAAACTTAA ACAAAGATTC ACTGTCTTTT CCACTTGTTA AACAGAACAG GGTATGCTCT 
GGTTCTGTCC TCGCAAGTGG TGAAGAGCTT CCTTCTGGCA TTGCAACCGC AGCTTTTGAT 
GGAATCCAAG AGTCCAAATG GGAAGAACCA AATGGTGCAA AAGGCTGTTG GATCGTGTAC 
AAAACACTCT ACAACCAGAT GCACCAACTC ATAGCATACG AACTCATGTC TGCTAATGAT 
GCCCCAGAGA GAGACCCCAA AGATTGGATT CTTGAAGGAA GTAACGATGG TGGTTCGACA 
TGGTGTGTCC TAGACAAGCA AACTAGTCAG GTATTCGAGG AACGGTTCCA ACGCAAATCC 
TACAAAATAA CTACACCTGG ATTCCAAGCA AATCTTTTCA GGTTCCGGTT TTTAAGTGTA 
CGAGACGTGA ATTCGACCTC GAGACTACAA CTAGGGAGCA TCGATCTGTA CAGAAGTCAC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199768.1
CDS	145..2310
Translation	MVARKFVVRHEDSSFDVDYNTEDGLEVLRFLIFSLTLVPPEEQKIVAEDDNRLVSDESDLASLSERLRLVSVGEDSVENSDAEMLKSDEELARMLQAEEDAIMFQQFVAARDNGEFEGRIRPYVSQVLMYEDPVRQDAARKTVPKDELEEKALVSLAKEGNFEPSKEERDYAFLLQLLFWFKKSFRWVNEPPCDFCGNKTIGQGMGNPLTSELAYGANRVEIYRCTMCPTTTRFPRYNDPLKLVETKKGRCGEWANCFTLYCRTFGYDSRLIMDFTDHVWTECYSHSLKRWIHLDPCEGVYDKPMLYEKGWNKKLNYVIAISKDGVCDVTKRYTKKWHEVLSRRTLTTESSLQDGLRTLTRERRRSLMFESLSKLELRDRNEQEELERNLHSADNASVSLPGRQSGDREWRIMRSEFGSDENSSVSSSSCPVRKCVDDHVTNIYDSFLPILTQFVEDGLPVARTNEVLKMIKQVLVDLKNAPYKTRKARLTLDSDNSSSFPEQFLPALGDLLLALSLKSERDTNGKSVTISVDGKLTKTAIALPVALDALRELVADLSKYQNLNKDSLSFPLVKQNRVCSGSVLASGEELPSGIATAAFDGIQESKWEEPNGAKGCWIVYKTLYNQMHQLIAYELMSANDAPERDPKDWILEGSNDGGSTWCVLDKQTSQVFEERFQRKSYKITTPGFQANLFRFRFLSVRDVNSTSRLQLGSIDLYRSHQ

Target ORF information:

RefSeq Version	NM_124335.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana peptide-N-glycanase 1 (PNG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124335.3

ORF Insert Sequence:

ATGGTTGCTC GGAAATTTGT CGTCCGCCAT GAAGATTCGA GCTTCGATGT CGACTACAAC 
ACTGAAGATG GTCTTGAGGT TTTACGATTT CTAATCTTCT CCCTCACTTT AGTTCCTCCT 
GAAGAACAAA AGATCGTTGC GGAAGATGAT AATCGATTGG TGTCTGATGA GTCTGATCTC 
GCTTCTTTAT CTGAGAGACT TCGATTAGTT TCAGTTGGAG AAGATTCTGT AGAGAACTCA 
GATGCGGAAA TGTTGAAATC AGATGAAGAA TTGGCTCGAA TGTTGCAGGC AGAAGAAGAT 
GCTATTATGT TTCAACAGTT TGTCGCTGCA CGAGATAACG GCGAATTCGA AGGGAGAATT 
AGGCCTTATG TTAGTCAAGT TCTCATGTAT GAAGATCCAG TCCGCCAAGA TGCTGCTCGA 
AAGACTGTTC CTAAAGACGA GCTTGAGGAG AAAGCATTGG TTTCTCTGGC CAAGGAAGGG 
AATTTTGAGC CATCGAAAGA GGAAAGAGAC TACGCTTTCC TGCTTCAGCT TCTTTTCTGG 
TTCAAAAAAT CCTTCAGATG GGTCAATGAA CCTCCTTGTG ACTTTTGTGG TAACAAAACC 
ATAGGCCAGG GGATGGGAAA CCCACTTACC TCGGAGCTTG CTTATGGAGC AAATCGAGTT 
GAAATATATC GCTGTACCAT GTGTCCAACA ACTACTCGAT TCCCTCGGTA CAACGATCCG 
CTAAAGCTCG TAGAAACAAA GAAAGGTCGT TGCGGAGAGT GGGCGAACTG CTTTACGCTA 
TACTGCCGCA CGTTTGGCTA TGATTCTCGT TTGATAATGG ACTTCACAGA TCACGTGTGG 
ACAGAATGCT ACTCTCACTC GTTAAAGAGG TGGATCCATC TTGACCCTTG TGAAGGAGTG 
TATGATAAAC CCATGTTATA TGAGAAAGGA TGGAATAAAA AATTGAATTA TGTCATTGCG 
ATTTCTAAGG ACGGGGTTTG TGATGTTACC AAGCGATATA CAAAGAAATG GCATGAGGTT 
TTATCTAGAA GAACTCTCAC AACTGAATCA TCTTTGCAAG ATGGTCTTCG AACCCTGACA 
AGGGAACGAC GACGTAGCTT AATGTTTGAA TCTCTATCTA AGCTTGAATT ACGAGACAGA 
AATGAGCAGG AAGAACTAGA GAGAAATCTG CATTCCGCAG ATAATGCCTC AGTTTCTTTA 
CCCGGAAGGC AAAGTGGGGA CAGGGAATGG CGTATTATGA GATCAGAATT TGGTTCAGAC 
GAAAACTCAT CTGTCAGCTC TTCCTCTTGC CCAGTTCGCA AATGCGTTGA TGACCATGTG 
ACCAACATTT ATGACTCGTT TCTACCTATC CTTACTCAGT TCGTTGAGGA CGGCTTACCT 
GTAGCAAGAA CAAATGAGGT ACTTAAGATG ATCAAGCAAG TCCTAGTGGA TCTGAAGAAT 
GCGCCTTACA AAACAAGGAA GGCTCGATTG ACTTTAGATT CAGATAACTC AAGTTCATTC 
CCGGAGCAGT TTCTTCCCGC ACTAGGCGAT TTGCTTCTCG CTCTTTCTTT AAAGAGCGAG 
AGAGACACAA ACGGTAAAAG TGTCACAATA TCCGTAGACG GGAAACTCAC TAAAACTGCT 
ATAGCATTGC CAGTTGCATT GGACGCGTTA AGGGAACTTG TTGCTGACCT CAGCAAATAC 
CAAAACTTAA ACAAAGATTC ACTGTCTTTT CCACTTGTTA AACAGAACAG GGTATGCTCT 
GGTTCTGTCC TCGCAAGTGG TGAAGAGCTT CCTTCTGGCA TTGCAACCGC AGCTTTTGAT 
GGAATCCAAG AGTCCAAATG GGAAGAACCA AATGGTGCAA AAGGCTGTTG GATCGTGTAC 
AAAACACTCT ACAACCAGAT GCACCAACTC ATAGCATACG AACTCATGTC TGCTAATGAT 
GCCCCAGAGA GAGACCCCAA AGATTGGATT CTTGAAGGAA GTAACGATGG TGGTTCGACA 
TGGTGTGTCC TAGACAAGCA AACTAGTCAG GTATTCGAGG AACGGTTCCA ACGCAAATCC 
TACAAAATAA CTACACCTGG ATTCCAAGCA AATCTTTTCA GGTTCCGGTT TTTAAGTGTA 
CGAGACGTGA ATTCGACCTC GAGACTACAA CTAGGGAGCA TCGATCTGTA CAGAAGTCAC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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PNG1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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