FRA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	FRA1
Entrez Gene ID	834833
Full Name	P-loop containing nucleoside triphosphate hydrolases superfamily protein
Synonyms	FRAGILE FIBER 1,MCA23.16,MCA23_16
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_180820.2	NP_851151.1	P-loop containing nucleoside triphosphate hydrolases superfamily protein
NM_124156.5	NP_199593.2	P-loop containing nucleoside triphosphate hydrolases superfamily protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIF4B	NP_001092763.1
	KIF4A	XP_002808485.1
Arabidopsis thaliana (thale cress)	FRA1	NP_199593.2
Pan troglodytes (chimpanzee)	KIF4B	XP_518055.3
Oryza sativa (rice)	Os09g0114500	NP_001062580.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a high density of processive motility is necessary for the physiological function of FRA1.
Title: The

FRA1 contributes to cell wall production by transporting Golgi-derived vesicles along cortical microtubules for secretion.
Title: The fragile Fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components.

FRA1 is a functional motor protein to drive long-distance transport of cargo along cortical microtubules.
Title: Single molecule analysis of the Arabidopsis FRA1 kinesin shows that it is a functional motor protein with unusually high processivity.

The following FRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20870	NM_180820.2 Latest version!	Arabidopsis thaliana P-loop containing nucleoside triphosphate hydrolases superfamily protein (FRA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb20870	NM_124156.5 Latest version!	Arabidopsis thaliana P-loop containing nucleoside triphosphate hydrolases superfamily protein (FRA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FRA1 ( NM_180820.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851151.1 Arabidopsis thaliana P-loop containing nucleoside triphosphate hydrolases superfamily protein (FRA1), mRNA.

Price & Availability↑

CloneID	OAb20870
Clone ID Related Accession (Same CDS sequence)	NM_180820.2 , NM_124156.5
Accession Version	NM_180820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	P-loop containing nucleoside triphosphate hydrolases superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_180820.1.

ATGGAATCTA CGCCGCCACC GGATGATTGT TCCGTTAAGG TTGCTGTTCA CATCAGACCA 
CTCATCGGCG ACGAAAGGAT TCAAGGTTGT CAAGATTGTG TCACTGTCGT TACCGGTAAA 
CCACAGGTAC AAATTGGATC ACATTCTTTT ACATTTGATC ATGTATATGG GAGCTCTGGT 
TCTCCATCTA CTGAAATGTA TGAAGAATGT GCTGCCCCTC TTGTTGATGG TTTATTTCAA 
GGCTACAATG CTACCGTTCT TGCTTATGGA CAAACGGGTT CCGGTAAAAC ATATACAATG 
GGTACTGGCT GCGGAGACAG CAGCCAGACT GGTATCATCC CTCAAGTTAT GAACGCTCTT 
TTCACCAAGA TAGAGACTTT GAAGCAGCAG ATTGAGTTTC AGATTCATGT TTCTTTTATT 
GAGATTCATA AAGAAGAGGT TCAAGATTTG CTGGATCCTT GTACTGTTAA TAAATCAGAC 
ACTAATAACA CTGGACATGT TGGTAAGGTA GCACATGTCC CTGGGAAGCC ACCGATACAG 
ATTCGAGAAA CATCCAACGG TGTTATCACA CTAGCTGGAT CCACAGAAGT AAGTGTTAGT 
ACGTTGAAAG AGATGGCTGC TTGCCTTGAC CAAGGTTCGG TTAGCAGGGC TACTGGTAGT 
ACTAACATGA ACAATCAGTC CAGTCGTTCA CATGCCATTT TCACTATCAC CGTGGAGCAA 
ATGCGCAAAA TTAATACGGA TTCTCCTGAA AACGGTGCTT ACAATGGGAG CTTGAAAGAA 
GAATATTTGT GTGCCAAGTT GCACTTAGTA GATCTTGCTG GTTCAGAACG AGCCAAAAGA 
ACTGGTTCAG ACGGTTTGAG ATTTAAGGAA GGAGTTCACA TCAACAAAGG CCTCCTTGCG 
CTTGGTAATG TTATCAGTGC GCTCGGTGAT GAGAAAAAGC GTAAAGATGG TGCCCATGTT 
CCCTACAGAG ACAGTAAACT TACACGGCTT TTGCAGGATT CCCTTGGTGG TAACAGCAGA 
ACTGTGATGA TAGCTTGCAT TAGTCCTGCA GATATTAACG CGGAGGAGAC ACTTAACACT 
TTGAAATATG CAAACCGTGC TCGAAACATC CGAAATAAAC CTGTTGTTAA TAGAGATCCT 
GTGTCTAGTG AGATGCTAAA AATGCGTCAA CAGGTAGAAT ACTTGCAGGC AGAACTCTCC 
TTACGGACCG GAGGATCCTC ATGTGCAGAA GTACAGGCTC TTAAAGAAAG GATTGTTTGG 
CTTGAAACTG CTAATGAAGA ACTTTGCCGC GAACTTCATG AGTACCGTAG CAGATGTCCT 
GGTGTAGAGC ATTCTGAAAA AGACTTTAAA GACATAAGAG CTGATGATAT TGTTGGTTCT 
GTCAGACCCG ATGGGCTGAA AAGGAGCTTG CACAGTATAG AGTCCTCTAA TTACCCCATG 
GTTGAAGCCA CAACAGGTGA TTCAAGGGAA ATTGATGAAG AAGCAAAGGA ATGGGAGCAT 
AAGCTCTTGC AAAATAGTAT GGACAAAGAG TTGTATGAAC TGAACCGCCG TCTAGAGGAA 
AAAGAGTCTG AAATGAAGCT GTTTGACGGC TATGATCCGG CTGCGTTAAA GCAGCATTTT 
GGAAAGAAAA TTGCAGAGGT GGAGGATGAA AAGAGATCAG TTCAGGAAGA AAGAAACCGT 
TTGTTGGCTG AAATTGAGAA CCTTGCTTCT GATGGTCAAG CACAGAAGTT GCAAGATGTT 
CATGCTCAGA ACTTAAAAGC GCTTGAAGCA CAGATTCTAG ACCTTAAGAA AAAGCAAGAG 
AGCCAAGTTC AGCTACTGAA ACAGAAACAA AAGAGTGATG ACGCGGCTCG GAGGTTACAA 
GATGAAATCC AATCCATCAA GGCACAAAAG GTTCAACTGC AGCACAGAAT GAAACAAGAA 
GCAGAACAGT TTCGACAGTG GAAAGCCTCC CGAGAGAAGG AACTTTTGCA GTTACGGAAA 
GAAGGGAGAA AGAGCGAGTA TGAAAGGCAT AAGCTGCAAG CTTTAAATCA GCGCCAGAAA 
ATGGTTCTTC AGAGGAAGAC AGAAGAGGCT GCAATGGCTA CCAAAAGGTT GAAAGAGTTG 
TTAGAAGCTC GAAAATCATC TCCTCGTGAA CACTCAGCTG GTACAAATGG CTTTGGAACA 
AATGGTCAGA CAAATGAGAA GTCATTGCAG CGTTGGCTTG ATCATGAGTT GGAAGTCATG 
GTAAATGTGC ATGAAGTACG TCATGAGTAT GAGAAACAAA GCCACGTACG AGCTGCACTG 
GCGGAAGAGT TGGCTGTTTT AAGACAAGTA GATGAGTTTG CAGTGAAAGG ATTAAGTCCT 
CCAAGAGGAA AGAATGGCTT TGCTAGGGCT TCATCGTTGT CACCTAATGC AAGGATGGCC 
CGTATATCTT CACTTGAGAA CATGCTCGTT ATATCTTCTA ATTCTTTAGT AGCGATGGCA 
TCACAACTTT CTGAGGCAGA AGAACGGGAG CGTGCTTTTA CAAACCGTGG TCGCTGGAAC 
CAGCTACGAT CAATGGGAGA GGCAAAGAAT TTGCTTCAGT ATATGTTCAA CTCTCTTGCA 
GAAACAAGGT GTCAATTATG GGAGAAGGAT GTAGAGATAA AAGAAATGAA AGATCAGTTC 
AAAGAAATTG TTGGTCTTCT GAGGCAGAGT GAGTTGAGAA GGAAAGAAGC AGAAAAGGAA 
TTAAAATTGA GGGAGCAAGC AATTGCAACT TCATTAGGTA CGCCGCCGAG TTCAGTGAAA 
CATGTAGCTG AAGACTTGAG CACACCTTCT CCAATGACCG TGCCAGCACA GAAACAGCTC 
AAATTTACGC CAGGGATTGC TAACGGAAAA GTGAGAGGAC CGGCTGCATT TCTTGATACA 
AACAAGAAGA TGGTACCGAT GGGACAAGTA TCGATGAGAA AACTATCAGC CGTAGGAAAA 
CAGGGTGGGA GACTTTGGAG GTGGAAGAGA AGTCATCACC AGTGGATTGT TCAATTCAAA 
TGGAAATGGC AAAAGCCATG GAGACTCTCT GAGTGGATTA GGACAAGTGA CGAAACACTC 
CTCAAGTCAA AACCCCGACT TAAGGCTCTA CCTAATAAGA TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851151.1
CDS	373..3480
Translation	MESTPPPDDCSVKVAVHIRPLIGDERIQGCQDCVTVVTGKPQVQIGSHSFTFDHVYGSSGSPSTEMYEECAAPLVDGLFQGYNATVLAYGQTGSGKTYTMGTGCGDSSQTGIIPQVMNALFTKIETLKQQIEFQIHVSFIEIHKEEVQDLLDPCTVNKSDTNNTGHVGKVAHVPGKPPIQIRETSNGVITLAGSTEVSVSTLKEMAACLDQGSVSRATGSTNMNNQSSRSHAIFTITVEQMRKINTDSPENGAYNGSLKEEYLCAKLHLVDLAGSERAKRTGSDGLRFKEGVHINKGLLALGNVISALGDEKKRKDGAHVPYRDSKLTRLLQDSLGGNSRTVMIACISPADINAEETLNTLKYANRARNIRNKPVVNRDPVSSEMLKMRQQVEYLQAELSLRTGGSSCAEVQALKERIVWLETANEELCRELHEYRSRCPGVEHSEKDFKDIRADDIVGSVRPDGLKRSLHSIESSNYPMVEATTGDSREIDEEAKEWEHKLLQNSMDKELYELNRRLEEKESEMKLFDGYDPAALKQHFGKKIAEVEDEKRSVQEERNRLLAEIENLASDGQAQKLQDVHAQNLKALEAQILDLKKKQESQVQLLKQKQKSDDAARRLQDEIQSIKAQKVQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQKMVLQRKTEEAAMATKRLKELLEARKSSPREHSAGTNGFGTNGQTNEKSLQRWLDHELEVMVNVHEVRHEYEKQSHVRAALAEELAVLRQVDEFAVKGLSPPRGKNGFARASSLSPNARMARISSLENMLVISSNSLVAMASQLSEAEERERAFTNRGRWNQLRSMGEAKNLLQYMFNSLAETRCQLWEKDVEIKEMKDQFKEIVGLLRQSELRRKEAEKELKLREQAIATSLGTPPSSVKHVAEDLSTPSPMTVPAQKQLKFTPGIANGKVRGPAAFLDTNKKMVPMGQVSMRKLSAVGKQGGRLWRWKRSHHQWIVQFKWKWQKPWRLSEWIRTSDETLLKSKPRLKALPNKIM

Target ORF information:

RefSeq Version	NM_180820.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana P-loop containing nucleoside triphosphate hydrolases superfamily protein (FRA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180820.2

ORF Insert Sequence:

ATGGAATCTA CGCCGCCACC GGATGATTGT TCCGTTAAGG TTGCTGTTCA CATCAGACCA 
CTCATCGGCG ACGAAAGGAT TCAAGGTTGT CAAGATTGTG TCACTGTCGT TACCGGTAAA 
CCACAGGTAC AAATTGGATC ACATTCTTTT ACATTTGATC ATGTATATGG GAGCTCTGGT 
TCTCCATCTA CTGAAATGTA TGAAGAATGT GCTGCCCCTC TTGTTGATGG TTTATTTCAA 
GGCTACAATG CTACCGTTCT TGCTTATGGA CAAACGGGTT CCGGTAAAAC ATATACAATG 
GGTACTGGCT GCGGAGACAG CAGCCAGACT GGTATCATCC CTCAAGTTAT GAACGCTCTT 
TTCACCAAGA TAGAGACTTT GAAGCAGCAG ATTGAGTTTC AGATTCATGT TTCTTTTATT 
GAGATTCATA AAGAAGAGGT TCAAGATTTG CTGGATCCTT GTACTGTTAA TAAATCAGAC 
ACTAATAACA CTGGACATGT TGGTAAGGTA GCACATGTCC CTGGGAAGCC ACCGATACAG 
ATTCGAGAAA CATCCAACGG TGTTATCACA CTAGCTGGAT CCACAGAAGT AAGTGTTAGT 
ACGTTGAAAG AGATGGCTGC TTGCCTTGAC CAAGGTTCGG TTAGCAGGGC TACTGGTAGT 
ACTAACATGA ACAATCAGTC CAGTCGTTCA CATGCCATTT TCACTATCAC CGTGGAGCAA 
ATGCGCAAAA TTAATACGGA TTCTCCTGAA AACGGTGCTT ACAATGGGAG CTTGAAAGAA 
GAATATTTGT GTGCCAAGTT GCACTTAGTA GATCTTGCTG GTTCAGAACG AGCCAAAAGA 
ACTGGTTCAG ACGGTTTGAG ATTTAAGGAA GGAGTTCACA TCAACAAAGG CCTCCTTGCG 
CTTGGTAATG TTATCAGTGC GCTCGGTGAT GAGAAAAAGC GTAAAGATGG TGCCCATGTT 
CCCTACAGAG ACAGTAAACT TACACGGCTT TTGCAGGATT CCCTTGGTGG TAACAGCAGA 
ACTGTGATGA TAGCTTGCAT TAGTCCTGCA GATATTAACG CGGAGGAGAC ACTTAACACT 
TTGAAATATG CAAACCGTGC TCGAAACATC CGAAATAAAC CTGTTGTTAA TAGAGATCCT 
GTGTCTAGTG AGATGCTAAA AATGCGTCAA CAGGTAGAAT ACTTGCAGGC AGAACTCTCC 
TTACGGACCG GAGGATCCTC ATGTGCAGAA GTACAGGCTC TTAAAGAAAG GATTGTTTGG 
CTTGAAACTG CTAATGAAGA ACTTTGCCGC GAACTTCATG AGTACCGTAG CAGATGTCCT 
GGTGTAGAGC ATTCTGAAAA AGACTTTAAA GACATAAGAG CTGATGATAT TGTTGGTTCT 
GTCAGACCCG ATGGGCTGAA AAGGAGCTTG CACAGTATAG AGTCCTCTAA TTACCCCATG 
GTTGAAGCCA CAACAGGTGA TTCAAGGGAA ATTGATGAAG AAGCAAAGGA ATGGGAGCAT 
AAGCTCTTGC AAAATAGTAT GGACAAAGAG TTGTATGAAC TGAACCGCCG TCTAGAGGAA 
AAAGAGTCTG AAATGAAGCT GTTTGACGGC TATGATCCGG CTGCGTTAAA GCAGCATTTT 
GGAAAGAAAA TTGCAGAGGT GGAGGATGAA AAGAGATCAG TTCAGGAAGA AAGAAACCGT 
TTGTTGGCTG AAATTGAGAA CCTTGCTTCT GATGGTCAAG CACAGAAGTT GCAAGATGTT 
CATGCTCAGA ACTTAAAAGC GCTTGAAGCA CAGATTCTAG ACCTTAAGAA AAAGCAAGAG 
AGCCAAGTTC AGCTACTGAA ACAGAAACAA AAGAGTGATG ACGCGGCTCG GAGGTTACAA 
GATGAAATCC AATCCATCAA GGCACAAAAG GTTCAACTGC AGCACAGAAT GAAACAAGAA 
GCAGAACAGT TTCGACAGTG GAAAGCCTCC CGAGAGAAGG AACTTTTGCA GTTACGGAAA 
GAAGGGAGAA AGAGCGAGTA TGAAAGGCAT AAGCTGCAAG CTTTAAATCA GCGCCAGAAA 
ATGGTTCTTC AGAGGAAGAC AGAAGAGGCT GCAATGGCTA CCAAAAGGTT GAAAGAGTTG 
TTAGAAGCTC GAAAATCATC TCCTCGTGAA CACTCAGCTG GTACAAATGG CTTTGGAACA 
AATGGTCAGA CAAATGAGAA GTCATTGCAG CGTTGGCTTG ATCATGAGTT GGAAGTCATG 
GTAAATGTGC ATGAAGTACG TCATGAGTAT GAGAAACAAA GCCACGTACG AGCTGCACTG 
GCGGAAGAGT TGGCTGTTTT AAGACAAGTA GATGAGTTTG CAGTGAAAGG ATTAAGTCCT 
CCAAGAGGAA AGAATGGCTT TGCTAGGGCT TCATCGTTGT CACCTAATGC AAGGATGGCC 
CGTATATCTT CACTTGAGAA CATGCTCGTT ATATCTTCTA ATTCTTTAGT AGCGATGGCA 
TCACAACTTT CTGAGGCAGA AGAACGGGAG CGTGCTTTTA CAAACCGTGG TCGCTGGAAC 
CAGCTACGAT CAATGGGAGA GGCAAAGAAT TTGCTTCAGT ATATGTTCAA CTCTCTTGCA 
GAAACAAGGT GTCAATTATG GGAGAAGGAT GTAGAGATAA AAGAAATGAA AGATCAGTTC 
AAAGAAATTG TTGGTCTTCT GAGGCAGAGT GAGTTGAGAA GGAAAGAAGC AGAAAAGGAA 
TTAAAATTGA GGGAGCAAGC AATTGCAACT TCATTAGGTA CGCCGCCGAG TTCAGTGAAA 
CATGTAGCTG AAGACTTGAG CACACCTTCT CCAATGACCG TGCCAGCACA GAAACAGCTC 
AAATTTACGC CAGGGATTGC TAACGGAAAA GTGAGAGGAC CGGCTGCATT TCTTGATACA 
AACAAGAAGA TGGTACCGAT GGGACAAGTA TCGATGAGAA AACTATCAGC CGTAGGAAAA 
CAGGGTGGGA GACTTTGGAG GTGGAAGAGA AGTCATCACC AGTGGATTGT TCAATTCAAA 
TGGAAATGGC AAAAGCCATG GAGACTCTCT GAGTGGATTA GGACAAGTGA CGAAACACTC 
CTCAAGTCAA AACCCCGACT TAAGGCTCTA CCTAATAAGA TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FRA1 ( NM_124156.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199593.2 Arabidopsis thaliana P-loop containing nucleoside triphosphate hydrolases superfamily protein (FRA1), mRNA.

Price & Availability↑

CloneID	OAb20870
Clone ID Related Accession (Same CDS sequence)	NM_180820.2 , NM_124156.5
Accession Version	NM_124156.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	P-loop containing nucleoside triphosphate hydrolases superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124156.4.

ATGGAATCTA CGCCGCCACC GGATGATTGT TCCGTTAAGG TTGCTGTTCA CATCAGACCA 
CTCATCGGCG ACGAAAGGAT TCAAGGTTGT CAAGATTGTG TCACTGTCGT TACCGGTAAA 
CCACAGGTAC AAATTGGATC ACATTCTTTT ACATTTGATC ATGTATATGG GAGCTCTGGT 
TCTCCATCTA CTGAAATGTA TGAAGAATGT GCTGCCCCTC TTGTTGATGG TTTATTTCAA 
GGCTACAATG CTACCGTTCT TGCTTATGGA CAAACGGGTT CCGGTAAAAC ATATACAATG 
GGTACTGGCT GCGGAGACAG CAGCCAGACT GGTATCATCC CTCAAGTTAT GAACGCTCTT 
TTCACCAAGA TAGAGACTTT GAAGCAGCAG ATTGAGTTTC AGATTCATGT TTCTTTTATT 
GAGATTCATA AAGAAGAGGT TCAAGATTTG CTGGATCCTT GTACTGTTAA TAAATCAGAC 
ACTAATAACA CTGGACATGT TGGTAAGGTA GCACATGTCC CTGGGAAGCC ACCGATACAG 
ATTCGAGAAA CATCCAACGG TGTTATCACA CTAGCTGGAT CCACAGAAGT AAGTGTTAGT 
ACGTTGAAAG AGATGGCTGC TTGCCTTGAC CAAGGTTCGG TTAGCAGGGC TACTGGTAGT 
ACTAACATGA ACAATCAGTC CAGTCGTTCA CATGCCATTT TCACTATCAC CGTGGAGCAA 
ATGCGCAAAA TTAATACGGA TTCTCCTGAA AACGGTGCTT ACAATGGGAG CTTGAAAGAA 
GAATATTTGT GTGCCAAGTT GCACTTAGTA GATCTTGCTG GTTCAGAACG AGCCAAAAGA 
ACTGGTTCAG ACGGTTTGAG ATTTAAGGAA GGAGTTCACA TCAACAAAGG CCTCCTTGCG 
CTTGGTAATG TTATCAGTGC GCTCGGTGAT GAGAAAAAGC GTAAAGATGG TGCCCATGTT 
CCCTACAGAG ACAGTAAACT TACACGGCTT TTGCAGGATT CCCTTGGTGG TAACAGCAGA 
ACTGTGATGA TAGCTTGCAT TAGTCCTGCA GATATTAACG CGGAGGAGAC ACTTAACACT 
TTGAAATATG CAAACCGTGC TCGAAACATC CGAAATAAAC CTGTTGTTAA TAGAGATCCT 
GTGTCTAGTG AGATGCTAAA AATGCGTCAA CAGGTAGAAT ACTTGCAGGC AGAACTCTCC 
TTACGGACCG GAGGATCCTC ATGTGCAGAA GTACAGGCTC TTAAAGAAAG GATTGTTTGG 
CTTGAAACTG CTAATGAAGA ACTTTGCCGC GAACTTCATG AGTACCGTAG CAGATGTCCT 
GGTGTAGAGC ATTCTGAAAA AGACTTTAAA GACATAAGAG CTGATGATAT TGTTGGTTCT 
GTCAGACCCG ATGGGCTGAA AAGGAGCTTG CACAGTATAG AGTCCTCTAA TTACCCCATG 
GTTGAAGCCA CAACAGGTGA TTCAAGGGAA ATTGATGAAG AAGCAAAGGA ATGGGAGCAT 
AAGCTCTTGC AAAATAGTAT GGACAAAGAG TTGTATGAAC TGAACCGCCG TCTAGAGGAA 
AAAGAGTCTG AAATGAAGCT GTTTGACGGC TATGATCCGG CTGCGTTAAA GCAGCATTTT 
GGAAAGAAAA TTGCAGAGGT GGAGGATGAA AAGAGATCAG TTCAGGAAGA AAGAAACCGT 
TTGTTGGCTG AAATTGAGAA CCTTGCTTCT GATGGTCAAG CACAGAAGTT GCAAGATGTT 
CATGCTCAGA ACTTAAAAGC GCTTGAAGCA CAGATTCTAG ACCTTAAGAA AAAGCAAGAG 
AGCCAAGTTC AGCTACTGAA ACAGAAACAA AAGAGTGATG ACGCGGCTCG GAGGTTACAA 
GATGAAATCC AATCCATCAA GGCACAAAAG GTTCAACTGC AGCACAGAAT GAAACAAGAA 
GCAGAACAGT TTCGACAGTG GAAAGCCTCC CGAGAGAAGG AACTTTTGCA GTTACGGAAA 
GAAGGGAGAA AGAGCGAGTA TGAAAGGCAT AAGCTGCAAG CTTTAAATCA GCGCCAGAAA 
ATGGTTCTTC AGAGGAAGAC AGAAGAGGCT GCAATGGCTA CCAAAAGGTT GAAAGAGTTG 
TTAGAAGCTC GAAAATCATC TCCTCGTGAA CACTCAGCTG GTACAAATGG CTTTGGAACA 
AATGGTCAGA CAAATGAGAA GTCATTGCAG CGTTGGCTTG ATCATGAGTT GGAAGTCATG 
GTAAATGTGC ATGAAGTACG TCATGAGTAT GAGAAACAAA GCCACGTACG AGCTGCACTG 
GCGGAAGAGT TGGCTGTTTT AAGACAAGTA GATGAGTTTG CAGTGAAAGG ATTAAGTCCT 
CCAAGAGGAA AGAATGGCTT TGCTAGGGCT TCATCGTTGT CACCTAATGC AAGGATGGCC 
CGTATATCTT CACTTGAGAA CATGCTCGTT ATATCTTCTA ATTCTTTAGT AGCGATGGCA 
TCACAACTTT CTGAGGCAGA AGAACGGGAG CGTGCTTTTA CAAACCGTGG TCGCTGGAAC 
CAGCTACGAT CAATGGGAGA GGCAAAGAAT TTGCTTCAGT ATATGTTCAA CTCTCTTGCA 
GAAACAAGGT GTCAATTATG GGAGAAGGAT GTAGAGATAA AAGAAATGAA AGATCAGTTC 
AAAGAAATTG TTGGTCTTCT GAGGCAGAGT GAGTTGAGAA GGAAAGAAGC AGAAAAGGAA 
TTAAAATTGA GGGAGCAAGC AATTGCAACT TCATTAGGTA CGCCGCCGAG TTCAGTGAAA 
CATGTAGCTG AAGACTTGAG CACACCTTCT CCAATGACCG TGCCAGCACA GAAACAGCTC 
AAATTTACGC CAGGGATTGC TAACGGAAAA GTGAGAGGAC CGGCTGCATT TCTTGATACA 
AACAAGAAGA TGGTACCGAT GGGACAAGTA TCGATGAGAA AACTATCAGC CGTAGGAAAA 
CAGGGTGGGA GACTTTGGAG GTGGAAGAGA AGTCATCACC AGTGGATTGT TCAATTCAAA 
TGGAAATGGC AAAAGCCATG GAGACTCTCT GAGTGGATTA GGACAAGTGA CGAAACACTC 
CTCAAGTCAA AACCCCGACT TAAGGCTCTA CCTAATAAGA TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199593.2
CDS	352..3459
Translation	MESTPPPDDCSVKVAVHIRPLIGDERIQGCQDCVTVVTGKPQVQIGSHSFTFDHVYGSSGSPSTEMYEECAAPLVDGLFQGYNATVLAYGQTGSGKTYTMGTGCGDSSQTGIIPQVMNALFTKIETLKQQIEFQIHVSFIEIHKEEVQDLLDPCTVNKSDTNNTGHVGKVAHVPGKPPIQIRETSNGVITLAGSTEVSVSTLKEMAACLDQGSVSRATGSTNMNNQSSRSHAIFTITVEQMRKINTDSPENGAYNGSLKEEYLCAKLHLVDLAGSERAKRTGSDGLRFKEGVHINKGLLALGNVISALGDEKKRKDGAHVPYRDSKLTRLLQDSLGGNSRTVMIACISPADINAEETLNTLKYANRARNIRNKPVVNRDPVSSEMLKMRQQVEYLQAELSLRTGGSSCAEVQALKERIVWLETANEELCRELHEYRSRCPGVEHSEKDFKDIRADDIVGSVRPDGLKRSLHSIESSNYPMVEATTGDSREIDEEAKEWEHKLLQNSMDKELYELNRRLEEKESEMKLFDGYDPAALKQHFGKKIAEVEDEKRSVQEERNRLLAEIENLASDGQAQKLQDVHAQNLKALEAQILDLKKKQESQVQLLKQKQKSDDAARRLQDEIQSIKAQKVQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQKMVLQRKTEEAAMATKRLKELLEARKSSPREHSAGTNGFGTNGQTNEKSLQRWLDHELEVMVNVHEVRHEYEKQSHVRAALAEELAVLRQVDEFAVKGLSPPRGKNGFARASSLSPNARMARISSLENMLVISSNSLVAMASQLSEAEERERAFTNRGRWNQLRSMGEAKNLLQYMFNSLAETRCQLWEKDVEIKEMKDQFKEIVGLLRQSELRRKEAEKELKLREQAIATSLGTPPSSVKHVAEDLSTPSPMTVPAQKQLKFTPGIANGKVRGPAAFLDTNKKMVPMGQVSMRKLSAVGKQGGRLWRWKRSHHQWIVQFKWKWQKPWRLSEWIRTSDETLLKSKPRLKALPNKIM

Target ORF information:

RefSeq Version	NM_124156.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana P-loop containing nucleoside triphosphate hydrolases superfamily protein (FRA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124156.5

ORF Insert Sequence:

ATGGAATCTA CGCCGCCACC GGATGATTGT TCCGTTAAGG TTGCTGTTCA CATCAGACCA 
CTCATCGGCG ACGAAAGGAT TCAAGGTTGT CAAGATTGTG TCACTGTCGT TACCGGTAAA 
CCACAGGTAC AAATTGGATC ACATTCTTTT ACATTTGATC ATGTATATGG GAGCTCTGGT 
TCTCCATCTA CTGAAATGTA TGAAGAATGT GCTGCCCCTC TTGTTGATGG TTTATTTCAA 
GGCTACAATG CTACCGTTCT TGCTTATGGA CAAACGGGTT CCGGTAAAAC ATATACAATG 
GGTACTGGCT GCGGAGACAG CAGCCAGACT GGTATCATCC CTCAAGTTAT GAACGCTCTT 
TTCACCAAGA TAGAGACTTT GAAGCAGCAG ATTGAGTTTC AGATTCATGT TTCTTTTATT 
GAGATTCATA AAGAAGAGGT TCAAGATTTG CTGGATCCTT GTACTGTTAA TAAATCAGAC 
ACTAATAACA CTGGACATGT TGGTAAGGTA GCACATGTCC CTGGGAAGCC ACCGATACAG 
ATTCGAGAAA CATCCAACGG TGTTATCACA CTAGCTGGAT CCACAGAAGT AAGTGTTAGT 
ACGTTGAAAG AGATGGCTGC TTGCCTTGAC CAAGGTTCGG TTAGCAGGGC TACTGGTAGT 
ACTAACATGA ACAATCAGTC CAGTCGTTCA CATGCCATTT TCACTATCAC CGTGGAGCAA 
ATGCGCAAAA TTAATACGGA TTCTCCTGAA AACGGTGCTT ACAATGGGAG CTTGAAAGAA 
GAATATTTGT GTGCCAAGTT GCACTTAGTA GATCTTGCTG GTTCAGAACG AGCCAAAAGA 
ACTGGTTCAG ACGGTTTGAG ATTTAAGGAA GGAGTTCACA TCAACAAAGG CCTCCTTGCG 
CTTGGTAATG TTATCAGTGC GCTCGGTGAT GAGAAAAAGC GTAAAGATGG TGCCCATGTT 
CCCTACAGAG ACAGTAAACT TACACGGCTT TTGCAGGATT CCCTTGGTGG TAACAGCAGA 
ACTGTGATGA TAGCTTGCAT TAGTCCTGCA GATATTAACG CGGAGGAGAC ACTTAACACT 
TTGAAATATG CAAACCGTGC TCGAAACATC CGAAATAAAC CTGTTGTTAA TAGAGATCCT 
GTGTCTAGTG AGATGCTAAA AATGCGTCAA CAGGTAGAAT ACTTGCAGGC AGAACTCTCC 
TTACGGACCG GAGGATCCTC ATGTGCAGAA GTACAGGCTC TTAAAGAAAG GATTGTTTGG 
CTTGAAACTG CTAATGAAGA ACTTTGCCGC GAACTTCATG AGTACCGTAG CAGATGTCCT 
GGTGTAGAGC ATTCTGAAAA AGACTTTAAA GACATAAGAG CTGATGATAT TGTTGGTTCT 
GTCAGACCCG ATGGGCTGAA AAGGAGCTTG CACAGTATAG AGTCCTCTAA TTACCCCATG 
GTTGAAGCCA CAACAGGTGA TTCAAGGGAA ATTGATGAAG AAGCAAAGGA ATGGGAGCAT 
AAGCTCTTGC AAAATAGTAT GGACAAAGAG TTGTATGAAC TGAACCGCCG TCTAGAGGAA 
AAAGAGTCTG AAATGAAGCT GTTTGACGGC TATGATCCGG CTGCGTTAAA GCAGCATTTT 
GGAAAGAAAA TTGCAGAGGT GGAGGATGAA AAGAGATCAG TTCAGGAAGA AAGAAACCGT 
TTGTTGGCTG AAATTGAGAA CCTTGCTTCT GATGGTCAAG CACAGAAGTT GCAAGATGTT 
CATGCTCAGA ACTTAAAAGC GCTTGAAGCA CAGATTCTAG ACCTTAAGAA AAAGCAAGAG 
AGCCAAGTTC AGCTACTGAA ACAGAAACAA AAGAGTGATG ACGCGGCTCG GAGGTTACAA 
GATGAAATCC AATCCATCAA GGCACAAAAG GTTCAACTGC AGCACAGAAT GAAACAAGAA 
GCAGAACAGT TTCGACAGTG GAAAGCCTCC CGAGAGAAGG AACTTTTGCA GTTACGGAAA 
GAAGGGAGAA AGAGCGAGTA TGAAAGGCAT AAGCTGCAAG CTTTAAATCA GCGCCAGAAA 
ATGGTTCTTC AGAGGAAGAC AGAAGAGGCT GCAATGGCTA CCAAAAGGTT GAAAGAGTTG 
TTAGAAGCTC GAAAATCATC TCCTCGTGAA CACTCAGCTG GTACAAATGG CTTTGGAACA 
AATGGTCAGA CAAATGAGAA GTCATTGCAG CGTTGGCTTG ATCATGAGTT GGAAGTCATG 
GTAAATGTGC ATGAAGTACG TCATGAGTAT GAGAAACAAA GCCACGTACG AGCTGCACTG 
GCGGAAGAGT TGGCTGTTTT AAGACAAGTA GATGAGTTTG CAGTGAAAGG ATTAAGTCCT 
CCAAGAGGAA AGAATGGCTT TGCTAGGGCT TCATCGTTGT CACCTAATGC AAGGATGGCC 
CGTATATCTT CACTTGAGAA CATGCTCGTT ATATCTTCTA ATTCTTTAGT AGCGATGGCA 
TCACAACTTT CTGAGGCAGA AGAACGGGAG CGTGCTTTTA CAAACCGTGG TCGCTGGAAC 
CAGCTACGAT CAATGGGAGA GGCAAAGAAT TTGCTTCAGT ATATGTTCAA CTCTCTTGCA 
GAAACAAGGT GTCAATTATG GGAGAAGGAT GTAGAGATAA AAGAAATGAA AGATCAGTTC 
AAAGAAATTG TTGGTCTTCT GAGGCAGAGT GAGTTGAGAA GGAAAGAAGC AGAAAAGGAA 
TTAAAATTGA GGGAGCAAGC AATTGCAACT TCATTAGGTA CGCCGCCGAG TTCAGTGAAA 
CATGTAGCTG AAGACTTGAG CACACCTTCT CCAATGACCG TGCCAGCACA GAAACAGCTC 
AAATTTACGC CAGGGATTGC TAACGGAAAA GTGAGAGGAC CGGCTGCATT TCTTGATACA 
AACAAGAAGA TGGTACCGAT GGGACAAGTA TCGATGAGAA AACTATCAGC CGTAGGAAAA 
CAGGGTGGGA GACTTTGGAG GTGGAAGAGA AGTCATCACC AGTGGATTGT TCAATTCAAA 
TGGAAATGGC AAAAGCCATG GAGACTCTCT GAGTGGATTA GGACAAGTGA CGAAACACTC 
CTCAAGTCAA AACCCCGACT TAAGGCTCTA CCTAATAAGA TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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FRA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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