GORK cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GORK
Entrez Gene ID	833728
Full Name	gated outwardly-rectifying K+ channel
Synonyms	MPA22.4,MPA22_4,gated outwardly-rectifying K+ channel
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001344207.1	NP_001332118.1	gated outwardly-rectifying K+ channel
NM_001344209.1	NP_001332119.1	gated outwardly-rectifying K+ channel
NM_001344208.1	NP_001332117.1	gated outwardly-rectifying K+ channel
NM_123109.5	NP_198566.2	gated outwardly-rectifying K+ channel

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	GORK	NP_198566.2
Arabidopsis thaliana (thale cress)	SKOR	NP_186934.1
Oryza sativa (rice)	Os04g0445000	NP_001052898.1
Drosophila melanogaster (fruit fly)	Ih	NP_001033948.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GORK is regulated by CPK33
Title: The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33.

The interaction of AtPP2CA with "phosphorylated" or "dephosphorylated" GORK systematically led to inhibition of the channel to the same baseline level.
Title: The Arabidopsis AtPP2CA Protein Phosphatase Inhibits the GORK K+ Efflux Channel and Exerts a Dominant Suppressive Effect on Phosphomimetic-activating Mutations.

GORK clustering in puncta is related to its gating and conductance, and reflects associated conformational changes and (de)stabilisation of the channel protein.
Title: Clustering of the K+ channel GORK of Arabidopsis parallels its gating by extracellular K+.

Sustained activation of K+ outward current could be the result of an unexpected post-transcriptional regulation of the guard cell outward-rectifying K+ (GORK) channels.
Title: Post-transcriptional regulation of GORK channels by superoxide anion contributes to increases in outward-rectifying K? currents.

The following GORK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GORK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb19882	NM_123109.5 Latest version!	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb41379	NM_001344209.1 Latest version!	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAb41380	NM_001344208.1 Latest version!	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAb41381	NM_001344207.1 Latest version!	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GORK ( NM_123109.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_198566.2 Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Price & Availability↑

CloneID	OAb19882
Clone ID Related Accession (Same CDS sequence)	NM_123109.5
Accession Version	NM_123109.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	gated outwardly-rectifying K+ channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123109.4.

ATGGGACGTC TCCGGAGACG GCAAGAGATA ATAGATCATG AAGAAGAGGA ATCAAACGAC 
GACGTTTCAT CAAGAAGAGG AAAACTCAGT TTAGCCGAGA CGTTTCGGTG GCTTGATTCA 
TCAGAGCATC GGAGAATTGA AACCGATGGT CATAATGATT ATAAATACAT CATTCATCCC 
AAAAACAGGT GGTACAAGGC ATGGGAAATG TTTATATTGG TGTGGGCAAT ATACTCCTCA 
TTGTTCACTC CCATGGAGTT TGGTTTCTTC CGCGGTCTGC CTGAGAGACT CTTTGTACTT 
GACATTGTTG GTCAGATCGC GTTCTTGGTC GATATTGTTC TTCAGTTCTT TGTTGCCTAT 
CGCGATACTC AGACCTACCG GACTGTCTAC AAACCAACAC GTATTGCTTT CCGGTACTTG 
AAGTCGCATT TTCTCATGGA TTTCATCGGT TGCTTCCCTT GGGATCTTAT TTATAAGGCA 
TCAGGGAAAC ATGAGTTGGT GAGGTACTTG TTGTGGATAA GGCTATTTCG GGTTCGCAAA 
GTGGTTGAGT TTTTCCAAAG GCTTGAGAAA GACACAAGAA TCAACTATCT ATTCACTAGA 
ATCTTAAAGC TCTTGTTCGT TGAAGTTTAT TGTACTCACA CTGCTGCTTG TATCTTCTAT 
TACTTGGCCA CCACTCTTCC TCCAGAAAAC GAAGGTTACA CTTGGATCGG TAGCTTGAAG 
CTAGGAGACT ATAGCTACGA GAATTTCCGA GAAATCGATC TCTGGAAACG TTATACTACC 
GCTCTATACT TCGCCATTGT CACTATGGCA ACTGTCGGTT ATGGAGACAT TCACGCGGTG 
AATCTGAGGG AAATGATATT CGTGATGATA TATGTTTCGT TTGATATGGT TCTCGGTGCT 
TACCTTATTG GTAACATCAC TGCCTTGATT GTGAAAGGTT CAAACACAGA GAGGTTCAGA 
GATAAAATGA ATGATCTCAT AAGTTTCATG AACCGCAAAA AACTCGGGAG AGACCTTCGT 
AGCCAGATAA CTGGTCATGT TAGATTGCAG TACGACAGTC ACTACACCGA CACTGTCATG 
CTTCAGGACA TCCCAGCATC AATCCGCGCC AAGATTGCGC AATTATTGTA TCTGCCTTAC 
ATCAAAAAAG TTCCTCTCTT CAAAGGCTGC TCCACAGAGT TTATTAATCA AATAGTTATA 
AGGCTCCATG AAGAGTATTT TCTTCCAGGA GAAGTAATAA CAGAGCAAGG AAACGTCGTG 
GATCATTTGT ATTTCGTCTG TGAAGGCTTA CTGGAGGCTC TTGTTACAAA AACAGATGGA 
TCAGAAGAGA GTGTGACGTT ACTTGGGCCT CACACTTCTT TTGGAGACAT CTCCATCATT 
TGCAACATTT CTCAACCTTT CACTGTTAGG GTTTGTGAGC TATGCCATCT TTTACGACTC 
GATAAACAGT CTTTCTCAAA CATCCTCGAG ATTTATTTTC ACGACGGACG CACAATCTTG 
AACAATATTA TGGAGGAGAA GGAATCAAAT GATAGGATAA AGAAGCTAGA ATCTGACATA 
GTGATTCACA TTGGGAAACA AGAAGCAGAA CTTGCATTGA AAGTAAACAG TGCAGCTTTC 
CAAGGAGATT TTTACCAGCT TAAGAGCTTA ATCCGATCTG GAGCCGATCC TAACAAAACC 
GATTACGATG GAAGATCACC GCTTCATCTT GCAGCATGTA GAGGCTATGA AGACATTACA 
TTATTCCTTA TTCAGGAAGG TGTTGATGTC AATCTAAAAG ATAAGTTCGG ACACACACCA 
TTGTTTGAGG CTGTGAAAGC AGGACAAGAA GGAGTGATTG GTTTGCTTGT CAAAGAAGGA 
GCCTCCTTTA ATTTAGAAGA TTCAGGAAAC TTCCTTTGCA CGACAGTTGC TAAAGGCGAC 
TCTGATTTTC TCAAGAGATT GCTTTCAAGC GGTATGAACC CAAACAGTGA AGATTATGAT 
CACAGAACGC CGCTTCATGT CGCGGCTTCT GAAGGGTTAT TCTTGATGGC TAAAATGTTG 
GTTGAAGCTG GAGCAAGCGT TATTTCTAAA GACCGATGGG GGAATTCTCC GCTTGATGAA 
GCCCGATTGT GCGGAAACAA GAAACTGATT AAGTTACTCG AAGATGTGAA AAATGCTCAG 
TCGTCTATCT ACCCGTCAAG CTTGCGTGAA TTACAAGAGG AGAGAATTGA GAGACGGAAA 
TGCACGGTGT TTCCATTCCA CCCGCAAGAG GCGAAAGAAG AGCGTAGTAG AAAGCACGGA 
GTTGTGGTTT GGATCCCAAG CAATCTCGAG AAACTCATAG TAACCGCTGC GAAAGAGCTA 
GGGCTATCGG ATGGAGCCTC ATTTGTACTA TTATCAGAAG ACCAAGGTCG TATCACAGAC 
ATTGATATGA TTAGTGATGG ACACAAATTG TATATGATCA GTGATACTAC TGATCAAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_198566.2
CDS	351..2813
Translation	MGRLRRRQEIIDHEEEESNDDVSSRRGKLSLAETFRWLDSSEHRRIETDGHNDYKYIIHPKNRWYKAWEMFILVWAIYSSLFTPMEFGFFRGLPERLFVLDIVGQIAFLVDIVLQFFVAYRDTQTYRTVYKPTRIAFRYLKSHFLMDFIGCFPWDLIYKASGKHELVRYLLWIRLFRVRKVVEFFQRLEKDTRINYLFTRILKLLFVEVYCTHTAACIFYYLATTLPPENEGYTWIGSLKLGDYSYENFREIDLWKRYTTALYFAIVTMATVGYGDIHAVNLREMIFVMIYVSFDMVLGAYLIGNITALIVKGSNTERFRDKMNDLISFMNRKKLGRDLRSQITGHVRLQYDSHYTDTVMLQDIPASIRAKIAQLLYLPYIKKVPLFKGCSTEFINQIVIRLHEEYFLPGEVITEQGNVVDHLYFVCEGLLEALVTKTDGSEESVTLLGPHTSFGDISIICNISQPFTVRVCELCHLLRLDKQSFSNILEIYFHDGRTILNNIMEEKESNDRIKKLESDIVIHIGKQEAELALKVNSAAFQGDFYQLKSLIRSGADPNKTDYDGRSPLHLAACRGYEDITLFLIQEGVDVNLKDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLEDSGNFLCTTVAKGDSDFLKRLLSSGMNPNSEDYDHRTPLHVAASEGLFLMAKMLVEAGASVISKDRWGNSPLDEARLCGNKKLIKLLEDVKNAQSSIYPSSLRELQEERIERRKCTVFPFHPQEAKEERSRKHGVVVWIPSNLEKLIVTAAKELGLSDGASFVLLSEDQGRITDIDMISDGHKLYMISDTTDQT

Target ORF information:

RefSeq Version	NM_123109.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123109.5

ORF Insert Sequence:

ATGGGACGTC TCCGGAGACG GCAAGAGATA ATAGATCATG AAGAAGAGGA ATCAAACGAC 
GACGTTTCAT CAAGAAGAGG AAAACTCAGT TTAGCCGAGA CGTTTCGGTG GCTTGATTCA 
TCAGAGCATC GGAGAATTGA AACCGATGGT CATAATGATT ATAAATACAT CATTCATCCC 
AAAAACAGGT GGTACAAGGC ATGGGAAATG TTTATATTGG TGTGGGCAAT ATACTCCTCA 
TTGTTCACTC CCATGGAGTT TGGTTTCTTC CGCGGTCTGC CTGAGAGACT CTTTGTACTT 
GACATTGTTG GTCAGATCGC GTTCTTGGTC GATATTGTTC TTCAGTTCTT TGTTGCCTAT 
CGCGATACTC AGACCTACCG GACTGTCTAC AAACCAACAC GTATTGCTTT CCGGTACTTG 
AAGTCGCATT TTCTCATGGA TTTCATCGGT TGCTTCCCTT GGGATCTTAT TTATAAGGCA 
TCAGGGAAAC ATGAGTTGGT GAGGTACTTG TTGTGGATAA GGCTATTTCG GGTTCGCAAA 
GTGGTTGAGT TTTTCCAAAG GCTTGAGAAA GACACAAGAA TCAACTATCT ATTCACTAGA 
ATCTTAAAGC TCTTGTTCGT TGAAGTTTAT TGTACTCACA CTGCTGCTTG TATCTTCTAT 
TACTTGGCCA CCACTCTTCC TCCAGAAAAC GAAGGTTACA CTTGGATCGG TAGCTTGAAG 
CTAGGAGACT ATAGCTACGA GAATTTCCGA GAAATCGATC TCTGGAAACG TTATACTACC 
GCTCTATACT TCGCCATTGT CACTATGGCA ACTGTCGGTT ATGGAGACAT TCACGCGGTG 
AATCTGAGGG AAATGATATT CGTGATGATA TATGTTTCGT TTGATATGGT TCTCGGTGCT 
TACCTTATTG GTAACATCAC TGCCTTGATT GTGAAAGGTT CAAACACAGA GAGGTTCAGA 
GATAAAATGA ATGATCTCAT AAGTTTCATG AACCGCAAAA AACTCGGGAG AGACCTTCGT 
AGCCAGATAA CTGGTCATGT TAGATTGCAG TACGACAGTC ACTACACCGA CACTGTCATG 
CTTCAGGACA TCCCAGCATC AATCCGCGCC AAGATTGCGC AATTATTGTA TCTGCCTTAC 
ATCAAAAAAG TTCCTCTCTT CAAAGGCTGC TCCACAGAGT TTATTAATCA AATAGTTATA 
AGGCTCCATG AAGAGTATTT TCTTCCAGGA GAAGTAATAA CAGAGCAAGG AAACGTCGTG 
GATCATTTGT ATTTCGTCTG TGAAGGCTTA CTGGAGGCTC TTGTTACAAA AACAGATGGA 
TCAGAAGAGA GTGTGACGTT ACTTGGGCCT CACACTTCTT TTGGAGACAT CTCCATCATT 
TGCAACATTT CTCAACCTTT CACTGTTAGG GTTTGTGAGC TATGCCATCT TTTACGACTC 
GATAAACAGT CTTTCTCAAA CATCCTCGAG ATTTATTTTC ACGACGGACG CACAATCTTG 
AACAATATTA TGGAGGAGAA GGAATCAAAT GATAGGATAA AGAAGCTAGA ATCTGACATA 
GTGATTCACA TTGGGAAACA AGAAGCAGAA CTTGCATTGA AAGTAAACAG TGCAGCTTTC 
CAAGGAGATT TTTACCAGCT TAAGAGCTTA ATCCGATCTG GAGCCGATCC TAACAAAACC 
GATTACGATG GAAGATCACC GCTTCATCTT GCAGCATGTA GAGGCTATGA AGACATTACA 
TTATTCCTTA TTCAGGAAGG TGTTGATGTC AATCTAAAAG ATAAGTTCGG ACACACACCA 
TTGTTTGAGG CTGTGAAAGC AGGACAAGAA GGAGTGATTG GTTTGCTTGT CAAAGAAGGA 
GCCTCCTTTA ATTTAGAAGA TTCAGGAAAC TTCCTTTGCA CGACAGTTGC TAAAGGCGAC 
TCTGATTTTC TCAAGAGATT GCTTTCAAGC GGTATGAACC CAAACAGTGA AGATTATGAT 
CACAGAACGC CGCTTCATGT CGCGGCTTCT GAAGGGTTAT TCTTGATGGC TAAAATGTTG 
GTTGAAGCTG GAGCAAGCGT TATTTCTAAA GACCGATGGG GGAATTCTCC GCTTGATGAA 
GCCCGATTGT GCGGAAACAA GAAACTGATT AAGTTACTCG AAGATGTGAA AAATGCTCAG 
TCGTCTATCT ACCCGTCAAG CTTGCGTGAA TTACAAGAGG AGAGAATTGA GAGACGGAAA 
TGCACGGTGT TTCCATTCCA CCCGCAAGAG GCGAAAGAAG AGCGTAGTAG AAAGCACGGA 
GTTGTGGTTT GGATCCCAAG CAATCTCGAG AAACTCATAG TAACCGCTGC GAAAGAGCTA 
GGGCTATCGG ATGGAGCCTC ATTTGTACTA TTATCAGAAG ACCAAGGTCG TATCACAGAC 
ATTGATATGA TTAGTGATGG ACACAAATTG TATATGATCA GTGATACTAC TGATCAAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GORK ( NM_001344209.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001332119.1 Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Price & Availability↑

CloneID	OAb41379
Clone ID Related Accession (Same CDS sequence)	NM_001344209.1
Accession Version	NM_001344209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	gated outwardly-rectifying K+ channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGACGTC TCCGGAGACG GCAAGAGATA ATAGATCATG AAGAAGAGGA ATCAAACGAC 
GACGTTTCAT CAAGAAGAGG AAAACTCAGT TTAGCCGAGA CGTTTCGGTG GCTTGATTCA 
TCAGAGCATC GGAGAATTGA AACCGATGGT CATAATGATT ATAAATACAT CATTCATCCC 
AAAAACAGGT GGTACAAGGC ATGGGAAATG TTTATATTGG TGTGGGCAAT ATACTCCTCA 
TTGTTCACTC CCATGGAGTT TGGTTTCTTC CGCGGTCTGC CTGAGAGACT CTTTGTACTT 
GACATTGTTG GTCAGATCGC GTTCTTGGTC GATATTGTTC TTCAGTTCTT TGTTGCCTAT 
CGCGATACTC AGACCTACCG GACTGTCTAC AAACCAACAC GTATTGCTTT CCGGTACTTG 
AAGTCGCATT TTCTCATGGA TTTCATCGGT TGCTTCCCTT GGGATCTTAT TTATAAGGCA 
TCAGGGAAAC ATGAGTTGGT GAGGTACTTG TTGTGGATAA GGCTATTTCG GGTTCGCAAA 
GTGGTTGAGT TTTTCCAAAG GCTTGAGAAA GACACAAGAA TCAACTATCT ATTCACTAGA 
ATCTTAAAGC TCTTGTTCGT TGAAGTTTAT TGTACTCACA CTGCTGCTTG TATCTTCTAT 
TACTTGGCCA CCACTCTTCC TCCAGAAAAC GAAGGTTACA CTTGGATCGG TAGCTTGAAG 
CTAGGAGACT ATAGCTACGA GAATTTCCGA GAAATCGATC TCTGGAAACG TTATACTACC 
GCTCTATACT TCGCCATTGT CACTATGGCA ACTGTCGGTT ATGGAGACAT TCACGCGGTG 
AATCTGAGGG AAATGATATT CGTGATGATA TATGTTTCGT TTGATATGGT TCTCGGTGCT 
TACCTTATTG GTAACATCAC TGCCTTGATT GTGAAAGGTT CAAACACAGA GAGGTTCAGA 
GATAAAATGA ATGATCTCAT AAGTTTCATG AACCGCAAAA AACTCGGGAG AGACCTTCGT 
AGCCAGATAA CTGGTCATGT TAGATTGCAG TACGACAGTC ACTACACCGA CACTGTCATG 
CTTCAGGACA TCCCAGCATC AATCCGCGCC AAGATTGCGC AATTATTGTA TCTGCCTTAC 
ATCAAAAAAG TTCCTCTCTT CAAAGGCTGC TCCACAGAGT TTATTAATCA AATAGTTATA 
AGGCTCCATG AAGAGTATTT TCTTCCAGGA GAAGTAATAA CAGAGCAAGG AAACGTCGTG 
GATCATTTGT ATTTCGTCTG TGAAGGCTTA CTGGAGGCTC TTGTTACAAA AACAGATGGA 
TCAGAAGAGA GTGTGACGTT ACTTGGGCCT CACACTTCTT TTGGAGACAT CTCCATCATT 
TGCAACATTT CTCAACCTTT CACTGTTAGG GTTTGTGAGC TATGCCATCT TTTACGACTC 
GATAAACAGT CTTTCTCAAA CATCCTCGAG ATTTATTTTC ACGACGGACG CACAATCTTG 
AACAATATTA TGGAGGAGAA GGAATCAAAT GATAGGATAA AGAAGCTAGA ATCTGACATA 
GTGATTCACA TTGGGAAACA AGAAGCAGAA CTTGCATTGA AAGTAAACAG TGCAGCTTTC 
CAAGGAGATT TTTACCAGCT TAAGAGCTTA ATCCGATCTG GAGCCGATCC TAACAAAACC 
GATTACGATG GAAGATCACC GCTTGTACGT TTGTCGCTTA TCGATTATCT TTCTTTTAAG 
AGCTTAACTT TGATTCTTTT TTATATCCAG CATCTTGCAG CATGTAGAGG CTATGAAGAC 
ATTACATTAT TCCTTATTCA GGAAGGTGTT GATGTCAATC TAAAAGATAA GTTCGGACAC 
ACACCATTGT TTGAGGCTGT GAAAGCAGGA CAAGAAGGAG TGATTGGTTT GCTTGTCAAA 
GAAGGAGCCT CCTTTAATTT AGAAGATTCA GGAAACTTCC TTTGCACGAC AGTTGCTAAA 
GGCGACTCTG ATTTTCTCAA GAGATTGCTT TCAAGCGGTA TGAACCCAAA CAGTGAAGAT 
TATGATCACA GAACGCCGCT TCATGTCGCG GCTTCTGAAG GGTTATTCTT GATGGCTAAA 
ATGTTGGTTG AAGCTGGAGC AAGCGTTATT TCTAAAGACC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332119.1
CDS	351..2495
Translation	MGRLRRRQEIIDHEEEESNDDVSSRRGKLSLAETFRWLDSSEHRRIETDGHNDYKYIIHPKNRWYKAWEMFILVWAIYSSLFTPMEFGFFRGLPERLFVLDIVGQIAFLVDIVLQFFVAYRDTQTYRTVYKPTRIAFRYLKSHFLMDFIGCFPWDLIYKASGKHELVRYLLWIRLFRVRKVVEFFQRLEKDTRINYLFTRILKLLFVEVYCTHTAACIFYYLATTLPPENEGYTWIGSLKLGDYSYENFREIDLWKRYTTALYFAIVTMATVGYGDIHAVNLREMIFVMIYVSFDMVLGAYLIGNITALIVKGSNTERFRDKMNDLISFMNRKKLGRDLRSQITGHVRLQYDSHYTDTVMLQDIPASIRAKIAQLLYLPYIKKVPLFKGCSTEFINQIVIRLHEEYFLPGEVITEQGNVVDHLYFVCEGLLEALVTKTDGSEESVTLLGPHTSFGDISIICNISQPFTVRVCELCHLLRLDKQSFSNILEIYFHDGRTILNNIMEEKESNDRIKKLESDIVIHIGKQEAELALKVNSAAFQGDFYQLKSLIRSGADPNKTDYDGRSPLVRLSLIDYLSFKSLTLILFYIQHLAACRGYEDITLFLIQEGVDVNLKDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLEDSGNFLCTTVAKGDSDFLKRLLSSGMNPNSEDYDHRTPLHVAASEGLFLMAKMLVEAGASVISKDR

Target ORF information:

RefSeq Version	NM_001344209.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344209.1

ORF Insert Sequence:

ATGGGACGTC TCCGGAGACG GCAAGAGATA ATAGATCATG AAGAAGAGGA ATCAAACGAC 
GACGTTTCAT CAAGAAGAGG AAAACTCAGT TTAGCCGAGA CGTTTCGGTG GCTTGATTCA 
TCAGAGCATC GGAGAATTGA AACCGATGGT CATAATGATT ATAAATACAT CATTCATCCC 
AAAAACAGGT GGTACAAGGC ATGGGAAATG TTTATATTGG TGTGGGCAAT ATACTCCTCA 
TTGTTCACTC CCATGGAGTT TGGTTTCTTC CGCGGTCTGC CTGAGAGACT CTTTGTACTT 
GACATTGTTG GTCAGATCGC GTTCTTGGTC GATATTGTTC TTCAGTTCTT TGTTGCCTAT 
CGCGATACTC AGACCTACCG GACTGTCTAC AAACCAACAC GTATTGCTTT CCGGTACTTG 
AAGTCGCATT TTCTCATGGA TTTCATCGGT TGCTTCCCTT GGGATCTTAT TTATAAGGCA 
TCAGGGAAAC ATGAGTTGGT GAGGTACTTG TTGTGGATAA GGCTATTTCG GGTTCGCAAA 
GTGGTTGAGT TTTTCCAAAG GCTTGAGAAA GACACAAGAA TCAACTATCT ATTCACTAGA 
ATCTTAAAGC TCTTGTTCGT TGAAGTTTAT TGTACTCACA CTGCTGCTTG TATCTTCTAT 
TACTTGGCCA CCACTCTTCC TCCAGAAAAC GAAGGTTACA CTTGGATCGG TAGCTTGAAG 
CTAGGAGACT ATAGCTACGA GAATTTCCGA GAAATCGATC TCTGGAAACG TTATACTACC 
GCTCTATACT TCGCCATTGT CACTATGGCA ACTGTCGGTT ATGGAGACAT TCACGCGGTG 
AATCTGAGGG AAATGATATT CGTGATGATA TATGTTTCGT TTGATATGGT TCTCGGTGCT 
TACCTTATTG GTAACATCAC TGCCTTGATT GTGAAAGGTT CAAACACAGA GAGGTTCAGA 
GATAAAATGA ATGATCTCAT AAGTTTCATG AACCGCAAAA AACTCGGGAG AGACCTTCGT 
AGCCAGATAA CTGGTCATGT TAGATTGCAG TACGACAGTC ACTACACCGA CACTGTCATG 
CTTCAGGACA TCCCAGCATC AATCCGCGCC AAGATTGCGC AATTATTGTA TCTGCCTTAC 
ATCAAAAAAG TTCCTCTCTT CAAAGGCTGC TCCACAGAGT TTATTAATCA AATAGTTATA 
AGGCTCCATG AAGAGTATTT TCTTCCAGGA GAAGTAATAA CAGAGCAAGG AAACGTCGTG 
GATCATTTGT ATTTCGTCTG TGAAGGCTTA CTGGAGGCTC TTGTTACAAA AACAGATGGA 
TCAGAAGAGA GTGTGACGTT ACTTGGGCCT CACACTTCTT TTGGAGACAT CTCCATCATT 
TGCAACATTT CTCAACCTTT CACTGTTAGG GTTTGTGAGC TATGCCATCT TTTACGACTC 
GATAAACAGT CTTTCTCAAA CATCCTCGAG ATTTATTTTC ACGACGGACG CACAATCTTG 
AACAATATTA TGGAGGAGAA GGAATCAAAT GATAGGATAA AGAAGCTAGA ATCTGACATA 
GTGATTCACA TTGGGAAACA AGAAGCAGAA CTTGCATTGA AAGTAAACAG TGCAGCTTTC 
CAAGGAGATT TTTACCAGCT TAAGAGCTTA ATCCGATCTG GAGCCGATCC TAACAAAACC 
GATTACGATG GAAGATCACC GCTTGTACGT TTGTCGCTTA TCGATTATCT TTCTTTTAAG 
AGCTTAACTT TGATTCTTTT TTATATCCAG CATCTTGCAG CATGTAGAGG CTATGAAGAC 
ATTACATTAT TCCTTATTCA GGAAGGTGTT GATGTCAATC TAAAAGATAA GTTCGGACAC 
ACACCATTGT TTGAGGCTGT GAAAGCAGGA CAAGAAGGAG TGATTGGTTT GCTTGTCAAA 
GAAGGAGCCT CCTTTAATTT AGAAGATTCA GGAAACTTCC TTTGCACGAC AGTTGCTAAA 
GGCGACTCTG ATTTTCTCAA GAGATTGCTT TCAAGCGGTA TGAACCCAAA CAGTGAAGAT 
TATGATCACA GAACGCCGCT TCATGTCGCG GCTTCTGAAG GGTTATTCTT GATGGCTAAA 
ATGTTGGTTG AAGCTGGAGC AAGCGTTATT TCTAAAGACC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GORK ( NM_001344208.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001332117.1 Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Price & Availability↑

CloneID	OAb41380
Clone ID Related Accession (Same CDS sequence)	NM_001344208.1
Accession Version	NM_001344208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	gated outwardly-rectifying K+ channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGAAAAGTG AGAAGAAATT GATCAGGTGG TACAAGGCAT GGGAAATGTT TATATTGGTG 
TGGGCAATAT ACTCCTCATT GTTCACTCCC ATGGAGTTTG GTTTCTTCCG CGGTCTGCCT 
GAGAGACTCT TTGTACTTGA CATTGTTGGT CAGATCGCGT TCTTGGTCGA TATTGTTCTT 
CAGTTCTTTG TTGCCTATCG CGATACTCAG ACCTACCGGA CTGTCTACAA ACCAACACGT 
ATTGCTTTCC GGTACTTGAA GTCGCATTTT CTCATGGATT TCATCGGTTG CTTCCCTTGG 
GATCTTATTT ATAAGGCATC AGGGAAACAT GAGTTGGTGA GGTACTTGTT GTGGATAAGG 
CTATTTCGGG TTCGCAAAGT GGTTGAGTTT TTCCAAAGGC TTGAGAAAGA CACAAGAATC 
AACTATCTAT TCACTAGAAT CTTAAAGCTC TTGTTCGTTG AAGTTTATTG TACTCACACT 
GCTGCTTGTA TCTTCTATTA CTTGGCCACC ACTCTTCCTC CAGAAAACGA AGGTTACACT 
TGGATCGGTA GCTTGAAGCT AGGAGACTAT AGCTACGAGA ATTTCCGAGA AATCGATCTC 
TGGAAACGTT ATACTACCGC TCTATACTTC GCCATTGTCA CTATGGCAAC TGTCGGTTAT 
GGAGACATTC ACGCGGTGAA TCTGAGGGAA ATGATATTCG TGATGATATA TGTTTCGTTT 
GATATGGTTC TCGGTGCTTA CCTTATTGGT AACATCACTG CCTTGATTGT GAAAGGTTCA 
AACACAGAGA GGTTCAGAGA TAAAATGAAT GATCTCATAA GTTTCATGAA CCGCAAAAAA 
CTCGGGAGAG ACCTTCGTAG CCAGATAACT GGTCATGTTA GATTGCAGTA CGACAGTCAC 
TACACCGACA CTGTCATGCT TCAGGACATC CCAGCATCAA TCCGCGCCAA GATTGCGCAA 
TTATTGTATC TGCCTTACAT CAAAAAAGTT CCTCTCTTCA AAGGCTGCTC CACAGAGTTT 
ATTAATCAAA TAGTTATAAG GCTCCATGAA GAGTATTTTC TTCCAGGAGA AGTAATAACA 
GAGCAAGGAA ACGTCGTGGA TCATTTGTAT TTCGTCTGTG AAGGCTTACT GGAGGCTCTT 
GTTACAAAAA CAGATGGATC AGAAGAGAGT GTGACGTTAC TTGGGCCTCA CACTTCTTTT 
GGAGACATCT CCATCATTTG CAACATTTCT CAACCTTTCA CTGTTAGGGT TTGTGAGCTA 
TGCCATCTTT TACGACTCGA TAAACAGTCT TTCTCAAACA TCCTCGAGAT TTATTTTCAC 
GACGGACGCA CAATCTTGAA CAATATTATG GAGGAGAAGG AATCAAATGA TAGGATAAAG 
AAGCTAGAAT CTGACATAGT GATTCACATT GGGAAACAAG AAGCAGAACT TGCATTGAAA 
GTAAACAGTG CAGCTTTCCA AGGAGATTTT TACCAGCTTA AGAGCTTAAT CCGATCTGGA 
GCCGATCCTA ACAAAACCGA TTACGATGGA AGATCACCGC TTGTACGTTT GTCGCTTATC 
GATTATCTTT CTTTTAAGAG CTTAACTTTG ATTCTTTTTT ATATCCAGCA TCTTGCAGCA 
TGTAGAGGCT ATGAAGACAT TACATTATTC CTTATTCAGG AAGGTGTTGA TGTCAATCTA 
AAAGATAAGT TCGGACACAC ACCATTGTTT GAGGCTGTGA AAGCAGGACA AGAAGGAGTG 
ATTGGTTTGC TTGTCAAAGA AGGAGCCTCC TTTAATTTAG AAGATTCAGG AAACTTCCTT 
TGCACGACAG TTGCTAAAGG CGACTCTGAT TTTCTCAAGA GATTGCTTTC AAGCGGTATG 
AACCCAAACA GTGAAGATTA TGATCACAGA ACGCCGCTTC ATGTCGCGGC TTCTGAAGGG 
TTATTCTTGA TGGCTAAAAT GTTGGTTGAA GCTGGAGCAA GCGTTATTTC TAAAGACCGA 
TGGGGGAATT CTCCGCTTGA TGAAGCCCGA TTGTGCGGAA ACAAGAAACT GATTAAGTTA 
CTCGAAGATG TGAAAAATGC TCAGTCGTCT ATCTACCCGT CAAGCTTGCG TGAATTACAA 
GAGGAGAGAA TTGAGAGACG GAAATGCACG GTGTTTCCAT TCCACCCGCA AGAGGCGAAA 
GAAGAGCGTA GTAGAAAGCA CGGAGTTGTG GTTTGGATCC CAAGCAATCT CGAGAAACTC 
ATAGTAACCG CTGCGAAAGA GCTAGGGCTA TCGGATGGAG CCTCATTTGT ACTATTATCA 
GAAGACCAAG GTCGTATCAC AGACATTGAT ATGATTAGTG ATGGACACAA ATTGTATATG 
ATCAGTGATA CTACTGATCA AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332117.1
CDS	225..2591
Translation	MKSEKKLIRWYKAWEMFILVWAIYSSLFTPMEFGFFRGLPERLFVLDIVGQIAFLVDIVLQFFVAYRDTQTYRTVYKPTRIAFRYLKSHFLMDFIGCFPWDLIYKASGKHELVRYLLWIRLFRVRKVVEFFQRLEKDTRINYLFTRILKLLFVEVYCTHTAACIFYYLATTLPPENEGYTWIGSLKLGDYSYENFREIDLWKRYTTALYFAIVTMATVGYGDIHAVNLREMIFVMIYVSFDMVLGAYLIGNITALIVKGSNTERFRDKMNDLISFMNRKKLGRDLRSQITGHVRLQYDSHYTDTVMLQDIPASIRAKIAQLLYLPYIKKVPLFKGCSTEFINQIVIRLHEEYFLPGEVITEQGNVVDHLYFVCEGLLEALVTKTDGSEESVTLLGPHTSFGDISIICNISQPFTVRVCELCHLLRLDKQSFSNILEIYFHDGRTILNNIMEEKESNDRIKKLESDIVIHIGKQEAELALKVNSAAFQGDFYQLKSLIRSGADPNKTDYDGRSPLVRLSLIDYLSFKSLTLILFYIQHLAACRGYEDITLFLIQEGVDVNLKDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLEDSGNFLCTTVAKGDSDFLKRLLSSGMNPNSEDYDHRTPLHVAASEGLFLMAKMLVEAGASVISKDRWGNSPLDEARLCGNKKLIKLLEDVKNAQSSIYPSSLRELQEERIERRKCTVFPFHPQEAKEERSRKHGVVVWIPSNLEKLIVTAAKELGLSDGASFVLLSEDQGRITDIDMISDGHKLYMISDTTDQT

Target ORF information:

RefSeq Version	NM_001344208.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344208.1

ORF Insert Sequence:

ATGAAAAGTG AGAAGAAATT GATCAGGTGG TACAAGGCAT GGGAAATGTT TATATTGGTG 
TGGGCAATAT ACTCCTCATT GTTCACTCCC ATGGAGTTTG GTTTCTTCCG CGGTCTGCCT 
GAGAGACTCT TTGTACTTGA CATTGTTGGT CAGATCGCGT TCTTGGTCGA TATTGTTCTT 
CAGTTCTTTG TTGCCTATCG CGATACTCAG ACCTACCGGA CTGTCTACAA ACCAACACGT 
ATTGCTTTCC GGTACTTGAA GTCGCATTTT CTCATGGATT TCATCGGTTG CTTCCCTTGG 
GATCTTATTT ATAAGGCATC AGGGAAACAT GAGTTGGTGA GGTACTTGTT GTGGATAAGG 
CTATTTCGGG TTCGCAAAGT GGTTGAGTTT TTCCAAAGGC TTGAGAAAGA CACAAGAATC 
AACTATCTAT TCACTAGAAT CTTAAAGCTC TTGTTCGTTG AAGTTTATTG TACTCACACT 
GCTGCTTGTA TCTTCTATTA CTTGGCCACC ACTCTTCCTC CAGAAAACGA AGGTTACACT 
TGGATCGGTA GCTTGAAGCT AGGAGACTAT AGCTACGAGA ATTTCCGAGA AATCGATCTC 
TGGAAACGTT ATACTACCGC TCTATACTTC GCCATTGTCA CTATGGCAAC TGTCGGTTAT 
GGAGACATTC ACGCGGTGAA TCTGAGGGAA ATGATATTCG TGATGATATA TGTTTCGTTT 
GATATGGTTC TCGGTGCTTA CCTTATTGGT AACATCACTG CCTTGATTGT GAAAGGTTCA 
AACACAGAGA GGTTCAGAGA TAAAATGAAT GATCTCATAA GTTTCATGAA CCGCAAAAAA 
CTCGGGAGAG ACCTTCGTAG CCAGATAACT GGTCATGTTA GATTGCAGTA CGACAGTCAC 
TACACCGACA CTGTCATGCT TCAGGACATC CCAGCATCAA TCCGCGCCAA GATTGCGCAA 
TTATTGTATC TGCCTTACAT CAAAAAAGTT CCTCTCTTCA AAGGCTGCTC CACAGAGTTT 
ATTAATCAAA TAGTTATAAG GCTCCATGAA GAGTATTTTC TTCCAGGAGA AGTAATAACA 
GAGCAAGGAA ACGTCGTGGA TCATTTGTAT TTCGTCTGTG AAGGCTTACT GGAGGCTCTT 
GTTACAAAAA CAGATGGATC AGAAGAGAGT GTGACGTTAC TTGGGCCTCA CACTTCTTTT 
GGAGACATCT CCATCATTTG CAACATTTCT CAACCTTTCA CTGTTAGGGT TTGTGAGCTA 
TGCCATCTTT TACGACTCGA TAAACAGTCT TTCTCAAACA TCCTCGAGAT TTATTTTCAC 
GACGGACGCA CAATCTTGAA CAATATTATG GAGGAGAAGG AATCAAATGA TAGGATAAAG 
AAGCTAGAAT CTGACATAGT GATTCACATT GGGAAACAAG AAGCAGAACT TGCATTGAAA 
GTAAACAGTG CAGCTTTCCA AGGAGATTTT TACCAGCTTA AGAGCTTAAT CCGATCTGGA 
GCCGATCCTA ACAAAACCGA TTACGATGGA AGATCACCGC TTGTACGTTT GTCGCTTATC 
GATTATCTTT CTTTTAAGAG CTTAACTTTG ATTCTTTTTT ATATCCAGCA TCTTGCAGCA 
TGTAGAGGCT ATGAAGACAT TACATTATTC CTTATTCAGG AAGGTGTTGA TGTCAATCTA 
AAAGATAAGT TCGGACACAC ACCATTGTTT GAGGCTGTGA AAGCAGGACA AGAAGGAGTG 
ATTGGTTTGC TTGTCAAAGA AGGAGCCTCC TTTAATTTAG AAGATTCAGG AAACTTCCTT 
TGCACGACAG TTGCTAAAGG CGACTCTGAT TTTCTCAAGA GATTGCTTTC AAGCGGTATG 
AACCCAAACA GTGAAGATTA TGATCACAGA ACGCCGCTTC ATGTCGCGGC TTCTGAAGGG 
TTATTCTTGA TGGCTAAAAT GTTGGTTGAA GCTGGAGCAA GCGTTATTTC TAAAGACCGA 
TGGGGGAATT CTCCGCTTGA TGAAGCCCGA TTGTGCGGAA ACAAGAAACT GATTAAGTTA 
CTCGAAGATG TGAAAAATGC TCAGTCGTCT ATCTACCCGT CAAGCTTGCG TGAATTACAA 
GAGGAGAGAA TTGAGAGACG GAAATGCACG GTGTTTCCAT TCCACCCGCA AGAGGCGAAA 
GAAGAGCGTA GTAGAAAGCA CGGAGTTGTG GTTTGGATCC CAAGCAATCT CGAGAAACTC 
ATAGTAACCG CTGCGAAAGA GCTAGGGCTA TCGGATGGAG CCTCATTTGT ACTATTATCA 
GAAGACCAAG GTCGTATCAC AGACATTGAT ATGATTAGTG ATGGACACAA ATTGTATATG 
ATCAGTGATA CTACTGATCA AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GORK ( NM_001344207.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001332118.1 Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Price & Availability↑

CloneID	OAb41381
Clone ID Related Accession (Same CDS sequence)	NM_001344207.1
Accession Version	NM_001344207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	gated outwardly-rectifying K+ channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGACGTC TCCGGAGACG GCAAGAGATA ATAGATCATG AAGAAGAGGA ATCAAACGAC 
GACGTTTCAT CAAGAAGAGG AAAACTCAGT TTAGCCGAGA CGTTTCGGTG GCTTGATTCA 
TCAGAGCATC GGAGAATTGA AACCGATGGT CATAATGATT ATAAATACAT CATTCATCCC 
AAAAACAGGT GGTACAAGGC ATGGGAAATG TTTATATTGG TGTGGGCAAT ATACTCCTCA 
TTGTTCACTC CCATGGAGTT TGGTTTCTTC CGCGGTCTGC CTGAGAGACT CTTTGTACTT 
GACATTGTTG GTCAGATCGC GTTCTTGGTC GATATTGTTC TTCAGTTCTT TGTTGCCTAT 
CGCGATACTC AGACCTACCG GACTGTCTAC AAACCAACAC GTATTGCTTT CCGGTACTTG 
AAGTCGCATT TTCTCATGGA TTTCATCGGT TGCTTCCCTT GGGATCTTAT TTATAAGGCA 
TCAGGGAAAC ATGAGTTGGT GAGGTACTTG TTGTGGATAA GGCTATTTCG GGTTCGCAAA 
GTGGTTGAGT TTTTCCAAAG GCTTGAGAAA GACACAAGAA TCAACTATCT ATTCACTAGA 
ATCTTAAAGC TCTTGTTCGT TGAAGTTTAT TGTACTCACA CTGCTGCTTG TATCTTCTAT 
TACTTGGCCA CCACTCTTCC TCCAGAAAAC GAAGGTTACA CTTGGATCGG TAGCTTGAAG 
CTAGGAGACT ATAGCTACGA GAATTTCCGA GAAATCGATC TCTGGAAACG TTATACTACC 
GCTCTATACT TCGCCATTGT CACTATGGCA ACTGTCGGTT ATGGAGACAT TCACGCGGTG 
AATCTGAGGG AAATGATATT CGTGATGATA TATGTTTCGT TTGATATGGT TCTCGGTGCT 
TACCTTATTG GTAACATCAC TGCCTTGATT GTGAAAGGTT CAAACACAGA GAGGTTCAGA 
GATAAAATGA ATGATCTCAT AAGTTTCATG AACCGCAAAA AACTCGGGAG AGACCTTCGT 
AGCCAGATAA CTGGTCATGT TAGATTGCAG TACGACAGTC ACTACACCGA CACTGTCATG 
CTTCAGGACA TCCCAGCATC AATCCGCGCC AAGATTGCGC AATTATTGTA TCTGCCTTAC 
ATCAAAAAAG TTCCTCTCTT CAAAGGCTGC TCCACAGAGT TTATTAATCA AATAGTTATA 
AGGCTCCATG AAGAGTATTT TCTTCCAGGA GAAGTAATAA CAGAGCAAGG AAACGTCGTG 
GATCATTTGT ATTTCGTCTG TGAAGGCTTA CTGGAGGCTC TTGTTACAAA AACAGATGGA 
TCAGAAGAGA GTGTGACGTT ACTTGGGCCT CACACTTCTT TTGGAGACAT CTCCATCATT 
TGCAACATTT CTCAACCTTT CACTGTTAGG GTTTGTGAGC TATGCCATCT TTTACGACTC 
GATAAACAGT CTTTCTCAAA CATCCTCGAG ATTTATTTTC ACGACGGACG CACAATCTTG 
AACAATATTA TGGAGGAGAA GGAATCAAAT GATAGGATAA AGAAGCTAGA ATCTGACATA 
GTGATTCACA TTGGGAAACA AGAAGCAGAA CTTGCATTGA AAGTAAACAG TGCAGCTTTC 
CAAGGAGATT TTTACCAGCT TAAGAGCTTA ATCCGATCTG GAGCCGATCC TAACAAAACC 
GATTACGATG GAAGATCACC GCTTGTACGT TTGTCGCTTA TCGATTATCT TTCTTTTAAG 
AGCTTAACTT TGATTCTTTT TTATATCCAG CATCTTGCAG CATGTAGAGG CTATGAAGAC 
ATTACATTAT TCCTTATTCA GGAAGGTGTT GATGTCAATC TAAAAGATAA GTTCGGACAC 
ACACCATTGT TTGAGGCTGT GAAAGCAGGA CAAGAAGGAG TGATTGGTTT GCTTGTCAAA 
GAAGGAGCCT CCTTTAATTT AGAAGATTCA GGAAACTTCC TTTGCACGAC AGTTGCTAAA 
GGCGACTCTG ATTTTCTCAA GAGATTGCTT TCAAGCGGTA TGAACCCAAA CAGTGAAGAT 
TATGATCACA GAACGCCGCT TCATGTCGCG GCTTCTGAAG GGTTATTCTT GATGGCTAAA 
ATGTTGGTTG AAGCTGGAGC AAGCGTTATT TCTAAAGACC GATGGGGGAA TTCTCCGCTT 
GATGAAGCCC GATTGTGCGG AAACAAGAAA CTGATTAAGT TACTCGAAGA TGTGAAAAAT 
GCTCAGTCGT CTATCTACCC GTCAAGCTTG CGTGAATTAC AAGAGGAGAG AATTGAGAGA 
CGGAAATGCA CGGTGTTTCC ATTCCACCCG CAAGAGGCGA AAGAAGAGCG TAGTAGAAAG 
CACGGAGTTG TGGTTTGGAT CCCAAGCAAT CTCGAGAAAC TCATAGTAAC CGCTGCGAAA 
GAGCTAGGGC TATCGGATGG AGCCTCATTT GTACTATTAT CAGAAGACCA AGGTCGTATC 
ACAGACATTG ATATGATTAG TGATGGACAC AAATTGTATA TGATCAGTGA TACTACTGAT 
CAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332118.1
CDS	351..2879
Translation	MGRLRRRQEIIDHEEEESNDDVSSRRGKLSLAETFRWLDSSEHRRIETDGHNDYKYIIHPKNRWYKAWEMFILVWAIYSSLFTPMEFGFFRGLPERLFVLDIVGQIAFLVDIVLQFFVAYRDTQTYRTVYKPTRIAFRYLKSHFLMDFIGCFPWDLIYKASGKHELVRYLLWIRLFRVRKVVEFFQRLEKDTRINYLFTRILKLLFVEVYCTHTAACIFYYLATTLPPENEGYTWIGSLKLGDYSYENFREIDLWKRYTTALYFAIVTMATVGYGDIHAVNLREMIFVMIYVSFDMVLGAYLIGNITALIVKGSNTERFRDKMNDLISFMNRKKLGRDLRSQITGHVRLQYDSHYTDTVMLQDIPASIRAKIAQLLYLPYIKKVPLFKGCSTEFINQIVIRLHEEYFLPGEVITEQGNVVDHLYFVCEGLLEALVTKTDGSEESVTLLGPHTSFGDISIICNISQPFTVRVCELCHLLRLDKQSFSNILEIYFHDGRTILNNIMEEKESNDRIKKLESDIVIHIGKQEAELALKVNSAAFQGDFYQLKSLIRSGADPNKTDYDGRSPLVRLSLIDYLSFKSLTLILFYIQHLAACRGYEDITLFLIQEGVDVNLKDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLEDSGNFLCTTVAKGDSDFLKRLLSSGMNPNSEDYDHRTPLHVAASEGLFLMAKMLVEAGASVISKDRWGNSPLDEARLCGNKKLIKLLEDVKNAQSSIYPSSLRELQEERIERRKCTVFPFHPQEAKEERSRKHGVVVWIPSNLEKLIVTAAKELGLSDGASFVLLSEDQGRITDIDMISDGHKLYMISDTTDQT

Target ORF information:

RefSeq Version	NM_001344207.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana gated outwardly-rectifying K+ channel (GORK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344207.1

ORF Insert Sequence:

ATGGGACGTC TCCGGAGACG GCAAGAGATA ATAGATCATG AAGAAGAGGA ATCAAACGAC 
GACGTTTCAT CAAGAAGAGG AAAACTCAGT TTAGCCGAGA CGTTTCGGTG GCTTGATTCA 
TCAGAGCATC GGAGAATTGA AACCGATGGT CATAATGATT ATAAATACAT CATTCATCCC 
AAAAACAGGT GGTACAAGGC ATGGGAAATG TTTATATTGG TGTGGGCAAT ATACTCCTCA 
TTGTTCACTC CCATGGAGTT TGGTTTCTTC CGCGGTCTGC CTGAGAGACT CTTTGTACTT 
GACATTGTTG GTCAGATCGC GTTCTTGGTC GATATTGTTC TTCAGTTCTT TGTTGCCTAT 
CGCGATACTC AGACCTACCG GACTGTCTAC AAACCAACAC GTATTGCTTT CCGGTACTTG 
AAGTCGCATT TTCTCATGGA TTTCATCGGT TGCTTCCCTT GGGATCTTAT TTATAAGGCA 
TCAGGGAAAC ATGAGTTGGT GAGGTACTTG TTGTGGATAA GGCTATTTCG GGTTCGCAAA 
GTGGTTGAGT TTTTCCAAAG GCTTGAGAAA GACACAAGAA TCAACTATCT ATTCACTAGA 
ATCTTAAAGC TCTTGTTCGT TGAAGTTTAT TGTACTCACA CTGCTGCTTG TATCTTCTAT 
TACTTGGCCA CCACTCTTCC TCCAGAAAAC GAAGGTTACA CTTGGATCGG TAGCTTGAAG 
CTAGGAGACT ATAGCTACGA GAATTTCCGA GAAATCGATC TCTGGAAACG TTATACTACC 
GCTCTATACT TCGCCATTGT CACTATGGCA ACTGTCGGTT ATGGAGACAT TCACGCGGTG 
AATCTGAGGG AAATGATATT CGTGATGATA TATGTTTCGT TTGATATGGT TCTCGGTGCT 
TACCTTATTG GTAACATCAC TGCCTTGATT GTGAAAGGTT CAAACACAGA GAGGTTCAGA 
GATAAAATGA ATGATCTCAT AAGTTTCATG AACCGCAAAA AACTCGGGAG AGACCTTCGT 
AGCCAGATAA CTGGTCATGT TAGATTGCAG TACGACAGTC ACTACACCGA CACTGTCATG 
CTTCAGGACA TCCCAGCATC AATCCGCGCC AAGATTGCGC AATTATTGTA TCTGCCTTAC 
ATCAAAAAAG TTCCTCTCTT CAAAGGCTGC TCCACAGAGT TTATTAATCA AATAGTTATA 
AGGCTCCATG AAGAGTATTT TCTTCCAGGA GAAGTAATAA CAGAGCAAGG AAACGTCGTG 
GATCATTTGT ATTTCGTCTG TGAAGGCTTA CTGGAGGCTC TTGTTACAAA AACAGATGGA 
TCAGAAGAGA GTGTGACGTT ACTTGGGCCT CACACTTCTT TTGGAGACAT CTCCATCATT 
TGCAACATTT CTCAACCTTT CACTGTTAGG GTTTGTGAGC TATGCCATCT TTTACGACTC 
GATAAACAGT CTTTCTCAAA CATCCTCGAG ATTTATTTTC ACGACGGACG CACAATCTTG 
AACAATATTA TGGAGGAGAA GGAATCAAAT GATAGGATAA AGAAGCTAGA ATCTGACATA 
GTGATTCACA TTGGGAAACA AGAAGCAGAA CTTGCATTGA AAGTAAACAG TGCAGCTTTC 
CAAGGAGATT TTTACCAGCT TAAGAGCTTA ATCCGATCTG GAGCCGATCC TAACAAAACC 
GATTACGATG GAAGATCACC GCTTGTACGT TTGTCGCTTA TCGATTATCT TTCTTTTAAG 
AGCTTAACTT TGATTCTTTT TTATATCCAG CATCTTGCAG CATGTAGAGG CTATGAAGAC 
ATTACATTAT TCCTTATTCA GGAAGGTGTT GATGTCAATC TAAAAGATAA GTTCGGACAC 
ACACCATTGT TTGAGGCTGT GAAAGCAGGA CAAGAAGGAG TGATTGGTTT GCTTGTCAAA 
GAAGGAGCCT CCTTTAATTT AGAAGATTCA GGAAACTTCC TTTGCACGAC AGTTGCTAAA 
GGCGACTCTG ATTTTCTCAA GAGATTGCTT TCAAGCGGTA TGAACCCAAA CAGTGAAGAT 
TATGATCACA GAACGCCGCT TCATGTCGCG GCTTCTGAAG GGTTATTCTT GATGGCTAAA 
ATGTTGGTTG AAGCTGGAGC AAGCGTTATT TCTAAAGACC GATGGGGGAA TTCTCCGCTT 
GATGAAGCCC GATTGTGCGG AAACAAGAAA CTGATTAAGT TACTCGAAGA TGTGAAAAAT 
GCTCAGTCGT CTATCTACCC GTCAAGCTTG CGTGAATTAC AAGAGGAGAG AATTGAGAGA 
CGGAAATGCA CGGTGTTTCC ATTCCACCCG CAAGAGGCGA AAGAAGAGCG TAGTAGAAAG 
CACGGAGTTG TGGTTTGGAT CCCAAGCAAT CTCGAGAAAC TCATAGTAAC CGCTGCGAAA 
GAGCTAGGGC TATCGGATGG AGCCTCATTT GTACTATTAT CAGAAGACCA AGGTCGTATC 
ACAGACATTG ATATGATTAG TGATGGACAC AAATTGTATA TGATCAGTGA TACTACTGAT 
CAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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GORK cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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