PEX4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEX4
Entrez Gene ID	832645
Full Name	peroxin4
Synonyms	F18A17.10,F18A17_10,UBC21,peroxin4,ubiquitin-conjugating enzyme 21
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001036862.2	NP_001031939.1	peroxin4
NM_122477.2	NP_568476.1	peroxin4

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	ubc16	NP_595078.1
Arabidopsis thaliana (thale cress)	PEX4	NP_568476.1
Oryza sativa (rice)	Os02g0634800	NP_001047515.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Growth at elevated temperature did not reduce the fraction of membrane-associated PEX5 in pex4-1 mutants, suggesting that elevated temperature did not restore PEX4 enzymatic function in the mutant.
Title: Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme.

PEX4 is necessary for Arabidopsis peroxisomal function. pex4 mutants are sucrose dependent during germination and display reduced responses to the auxin precursor indole-3-butyric acid, presumably due to peroxisomal beta-oxidation defects.
Title: Identification and functional characterization of Arabidopsis PEROXIN4 and the interacting protein PEROXIN22.

The following PEX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19536	NM_122477.2 Latest version!	Arabidopsis thaliana peroxin4 (PEX4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAb19536	NM_001036862.2 Latest version!	Arabidopsis thaliana peroxin4 (PEX4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEX4 ( NM_001036862.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031939.1 Arabidopsis thaliana peroxin4 (PEX4), mRNA.

Price & Availability↑

CloneID	OAb19536
Clone ID Related Accession (Same CDS sequence)	NM_001036862.2 , NM_122477.2
Accession Version	NM_001036862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	peroxin4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001036862.1.

ATGCAGGCAT CAAGAGCGCG ACTGTTTAAA GAATACAAAG AGGTACAGCG AGAGAAAGTA 
GCAGATCCTG ATATTCAATT GATCTGTGAC GATACCAACA TATTCAAATG GACCGCTCTT 
ATCAAAGGAC CTTCGGAGAC TCCTTACGAA GGCGGTGTTT TTCAGCTTGC TTTTTCTGTT 
CCTGAACCTT ATCCTTTGCA ACCTCCTCAA GTTCGATTCT TGACCAAGAT ATTCCATCCT 
AATGTTCATT TCAAGACAGG AGAAATATGT CTCGACATTT TGAAGAATGC TTGGAGTCCT 
GCTTGGACGC TTCAGTCTGT GTGTAGAGCT ATCATAGCAT TGATGGCTCA TCCTGAGCCG 
GACAGTCCTC TTAACTGCGA CTCAGGGAAT CTTCTAAGAT CTGGTGATGT GAGAGGGTTC 
AATTCAATGG CACAAATGTA TACACGCCTC GCCGCTATGC CTAAGAAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031939.1
CDS	289..762
Translation	MQASRARLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG

Target ORF information:

RefSeq Version	NM_001036862.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana peroxin4 (PEX4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036862.2

ORF Insert Sequence:

ATGCAGGCAT CAAGAGCGCG ACTGTTTAAA GAATACAAAG AGGTACAGCG AGAGAAAGTA 
GCAGATCCTG ATATTCAATT GATCTGTGAC GATACCAACA TATTCAAATG GACCGCTCTT 
ATCAAAGGAC CTTCGGAGAC TCCTTACGAA GGCGGTGTTT TTCAGCTTGC TTTTTCTGTT 
CCTGAACCTT ATCCTTTGCA ACCTCCTCAA GTTCGATTCT TGACCAAGAT ATTCCATCCT 
AATGTTCATT TCAAGACAGG AGAAATATGT CTCGACATTT TGAAGAATGC TTGGAGTCCT 
GCTTGGACGC TTCAGTCTGT GTGTAGAGCT ATCATAGCAT TGATGGCTCA TCCTGAGCCG 
GACAGTCCTC TTAACTGCGA CTCAGGGAAT CTTCTAAGAT CTGGTGATGT GAGAGGGTTC 
AATTCAATGG CACAAATGTA TACACGCCTC GCCGCTATGC CTAAGAAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX4 ( NM_122477.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568476.1 Arabidopsis thaliana peroxin4 (PEX4), mRNA.

Price & Availability↑

CloneID	OAb19536
Clone ID Related Accession (Same CDS sequence)	NM_001036862.2 , NM_122477.2
Accession Version	NM_122477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	peroxin4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 18, 2007 this sequence version replaced NM_122477.1.

ATGCAGGCAT CAAGAGCGCG ACTGTTTAAA GAATACAAAG AGGTACAGCG AGAGAAAGTA 
GCAGATCCTG ATATTCAATT GATCTGTGAC GATACCAACA TATTCAAATG GACCGCTCTT 
ATCAAAGGAC CTTCGGAGAC TCCTTACGAA GGCGGTGTTT TTCAGCTTGC TTTTTCTGTT 
CCTGAACCTT ATCCTTTGCA ACCTCCTCAA GTTCGATTCT TGACCAAGAT ATTCCATCCT 
AATGTTCATT TCAAGACAGG AGAAATATGT CTCGACATTT TGAAGAATGC TTGGAGTCCT 
GCTTGGACGC TTCAGTCTGT GTGTAGAGCT ATCATAGCAT TGATGGCTCA TCCTGAGCCG 
GACAGTCCTC TTAACTGCGA CTCAGGGAAT CTTCTAAGAT CTGGTGATGT GAGAGGGTTC 
AATTCAATGG CACAAATGTA TACACGCCTC GCCGCTATGC CTAAGAAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568476.1
CDS	96..569
Translation	MQASRARLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG

Target ORF information:

RefSeq Version	NM_122477.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana peroxin4 (PEX4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122477.2

ORF Insert Sequence:

ATGCAGGCAT CAAGAGCGCG ACTGTTTAAA GAATACAAAG AGGTACAGCG AGAGAAAGTA 
GCAGATCCTG ATATTCAATT GATCTGTGAC GATACCAACA TATTCAAATG GACCGCTCTT 
ATCAAAGGAC CTTCGGAGAC TCCTTACGAA GGCGGTGTTT TTCAGCTTGC TTTTTCTGTT 
CCTGAACCTT ATCCTTTGCA ACCTCCTCAA GTTCGATTCT TGACCAAGAT ATTCCATCCT 
AATGTTCATT TCAAGACAGG AGAAATATGT CTCGACATTT TGAAGAATGC TTGGAGTCCT 
GCTTGGACGC TTCAGTCTGT GTGTAGAGCT ATCATAGCAT TGATGGCTCA TCCTGAGCCG 
GACAGTCCTC TTAACTGCGA CTCAGGGAAT CTTCTAAGAT CTGGTGATGT GAGAGGGTTC 
AATTCAATGG CACAAATGTA TACACGCCTC GCCGCTATGC CTAAGAAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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PEX4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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