SIGE cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIGE
Entrez Gene ID	832477
Full Name	sigma factor E
Synonyms	ATSIG5,MLE8.4,MLE8_4,SIG5,SIGMA FACTOR,SIGMA FACTOR 5,sigma factor E
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001343841.1	NP_001330409.1	sigma factor E
NM_001343842.1	NP_001330408.1	sigma factor E
NM_122317.4	NP_197800.1	sigma factor E

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0586600	NP_001056463.1
Arabidopsis thaliana (thale cress)	SIGE	NP_197800.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The role of SIG5 protein in the transcription of chloroplast genes via protein phosphatase 2C-dependent activation of nuclear genes
Title: Abscisic acid affects transcription of chloroplast genes via protein phosphatase 2C-dependent activation of nuclear genes: repression by guanosine-3'-5'-bisdiphosphate and activation by sigma factor 5.

nuclear-encoded SIGMA FACTOR5 (SIG5) controls circadian rhythms of transcription of several chloroplast genes, revealing one pathway by which the nuclear-encoded circadian oscillator controls rhythms of chloroplast gene expression.
Title: Circadian control of chloroplast transcription by a nuclear-encoded timing signal.

Cryptochromes 1 and 2 mediate SIG5 transcription at different light wavelengths. SIG5 activates the psbD blue-light-responsive promoter.
Title: Light induction of Arabidopsis SIG1 and SIG5 transcripts in mature leaves: differential roles of cryptochrome 1 and cryptochrome 2 and dual function of SIG5 in the recognition of plastid promoters.

The NEP (the (phage-type) plastid RNA polymerase) promoter cluster can help maintain RNA synthesis in situations where no functional sigma factor is available for PEP (the bacterial-type plastid RNA polymerase).
Title: A promoter switch that can rescue a plant sigma factor mutant.

The following SIGE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIGE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb19398	NM_001343842.1 Latest version!	Arabidopsis thaliana sigma factor E (SIGE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb19398	NM_122317.4 Latest version!	Arabidopsis thaliana sigma factor E (SIGE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb19398	NM_001343841.1 Latest version!	Arabidopsis thaliana sigma factor E (SIGE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIGE ( NM_001343841.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330409.1 Arabidopsis thaliana sigma factor E (SIGE), mRNA.

Price & Availability↑

CloneID	OAb19398
Clone ID Related Accession (Same CDS sequence)	NM_001343841.1 , NM_001343842.1 , NM_122317.4
Accession Version	NM_001343841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	sigma factor E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGAGTTG TGTCTATTTC AAGTTCAGCT GCAAGATCTC CACTCGGGTT GGGCAACGAT 
CTTTTGACAC ATCGGTCTTC ACTTAAAAAG CCATCCATTG TTGCTTTTAA AGCCGATGAT 
TCCAGCAATT CAGCTTTGAT CATTCCTCCT CGTGAGCAAG TTTTGATTCC TGCTGAAAAG 
CATAATGAGA AGAAGAGAGA AGTAACGAGG AGAAAGCCTT GTAAAACCCC GAAAAAACCG 
CTTTCCTTGG AACATAATTC GGCTCCTTCG TGTTCGTTAG GAGTAGATTA CAATGAAGCT 
GCTGCAAGAC TTGAAAGCAT ATACAAGCTT AGTCCTGCAA CAACCACATT AGTCGAGGAT 
GATGTTGAAG ATGGCGGCTC TAAAGCCAAA GTTTCACGGA GGAGAAAAAG GAAAGAAAGT 
GGTGAGGAGA AGAAAGTGGT TGTGAGGAAT AATGTGAAGA AGGAAAAACG TATGAGTCTT 
GATAAACGGA TTGCTTTGAA GAGAAACGTT CAAGAGAAAC CAGTCGTTGC TTCTGTTGAT 
AAGAAAGTAA CAAAGAGGCA GCAAGAAGAG GAGAAGATCG AGAGGCTTGT GCGGGACTAT 
TCAGCTTCTA ATGATATAGT GAGCTTGGAC TGGAAAAAAA TGAAGATACC TCCTGTTCTT 
TCTTCTACTG AACATGCTTG GTTGTTCAAG TTGATGCAAC CTATGAAGGC TCTTCTTGAA 
GTGAAAGATG TATTGCAAAA GAGCTTGGGA AGAGAGCCTA GGGAAGCTGA AATAGCCGGG 
GAGATCAATA TGACTGCGGG TGAAGTAAAA AAGAAAATCG AAATAGGTAG AGCTGCAAGA 
AACAAACTTA TTAAGCACAA TCTCCGGCTT GTCTTGTTTG TTATGAACAA ATACTTTCAT 
GAGTTTACCA ACGGACCAAA ATTCCAAGAC CTTTGTCAAG CGGGTATGAG AGGGCTTATC 
ACAGCAATTG ACCGCTTTGA GCCGAAAAGG AAGTTTCGTC TTTCGACTTA TGGTTTGTTT 
TGGATTAGAC ATGCCATCAT ACGGTCAATG ACAACTTCAA ACTTCACTCG TGTCCCATTT 
GGACTTGAAT CGGTTAGAGT GGAGATATAT AAAGCAAAGA CTGAGCTATG GTTTGAGATG 
GGAAGACCTC CAACGGAGGA CGAGGTAGTT GAGAGACTCA AGATAACACC TGAGAGATAC 
CGTGAAGTAT TGAGGGCTGC AAAACCGGTT TTGTCACTAA ATTCAAAACA TTCGGTTACT 
CAAGAAGAAT TCATCAACGG AATCACAGAT GTTGATGGTG TTGGAGCTAA TAACAGCAGA 
CAGCCTGCTC TTCTCAGGCT CGCTCTCGAC GATGTGCTCG ATTCATTGAA GCCGAAAGAG 
AGTCTGGTTA TTCGACAGAG GTACGGTCTT GATGGAAAAG GCGACAGGAC TCTAGGAGAG 
ATAGCTGGAA ATCTCAACAT CTCTAGAGAA ATGGTTAGGA AACATGAAGT CAAGGCCTTG 
ATGAAGCTTA AGCATCAGGC CAGAGTTGAT TACCTTCGTC AATACATCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330409.1
CDS	222..1775
Translation	MGVVSISSSAARSPLGLGNDLLTHRSSLKKPSIVAFKADDSSNSALIIPPREQVLIPAEKHNEKKREVTRRKPCKTPKKPLSLEHNSAPSCSLGVDYNEAAARLESIYKLSPATTTLVEDDVEDGGSKAKVSRRRKRKESGEEKKVVVRNNVKKEKRMSLDKRIALKRNVQEKPVVASVDKKVTKRQQEEEKIERLVRDYSASNDIVSLDWKKMKIPPVLSSTEHAWLFKLMQPMKALLEVKDVLQKSLGREPREAEIAGEINMTAGEVKKKIEIGRAARNKLIKHNLRLVLFVMNKYFHEFTNGPKFQDLCQAGMRGLITAIDRFEPKRKFRLSTYGLFWIRHAIIRSMTTSNFTRVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLKITPERYREVLRAAKPVLSLNSKHSVTQEEFINGITDVDGVGANNSRQPALLRLALDDVLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRKHEVKALMKLKHQARVDYLRQYIV

Target ORF information:

RefSeq Version	NM_001343841.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana sigma factor E (SIGE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343841.1

ORF Insert Sequence:

ATGGGAGTTG TGTCTATTTC AAGTTCAGCT GCAAGATCTC CACTCGGGTT GGGCAACGAT 
CTTTTGACAC ATCGGTCTTC ACTTAAAAAG CCATCCATTG TTGCTTTTAA AGCCGATGAT 
TCCAGCAATT CAGCTTTGAT CATTCCTCCT CGTGAGCAAG TTTTGATTCC TGCTGAAAAG 
CATAATGAGA AGAAGAGAGA AGTAACGAGG AGAAAGCCTT GTAAAACCCC GAAAAAACCG 
CTTTCCTTGG AACATAATTC GGCTCCTTCG TGTTCGTTAG GAGTAGATTA CAATGAAGCT 
GCTGCAAGAC TTGAAAGCAT ATACAAGCTT AGTCCTGCAA CAACCACATT AGTCGAGGAT 
GATGTTGAAG ATGGCGGCTC TAAAGCCAAA GTTTCACGGA GGAGAAAAAG GAAAGAAAGT 
GGTGAGGAGA AGAAAGTGGT TGTGAGGAAT AATGTGAAGA AGGAAAAACG TATGAGTCTT 
GATAAACGGA TTGCTTTGAA GAGAAACGTT CAAGAGAAAC CAGTCGTTGC TTCTGTTGAT 
AAGAAAGTAA CAAAGAGGCA GCAAGAAGAG GAGAAGATCG AGAGGCTTGT GCGGGACTAT 
TCAGCTTCTA ATGATATAGT GAGCTTGGAC TGGAAAAAAA TGAAGATACC TCCTGTTCTT 
TCTTCTACTG AACATGCTTG GTTGTTCAAG TTGATGCAAC CTATGAAGGC TCTTCTTGAA 
GTGAAAGATG TATTGCAAAA GAGCTTGGGA AGAGAGCCTA GGGAAGCTGA AATAGCCGGG 
GAGATCAATA TGACTGCGGG TGAAGTAAAA AAGAAAATCG AAATAGGTAG AGCTGCAAGA 
AACAAACTTA TTAAGCACAA TCTCCGGCTT GTCTTGTTTG TTATGAACAA ATACTTTCAT 
GAGTTTACCA ACGGACCAAA ATTCCAAGAC CTTTGTCAAG CGGGTATGAG AGGGCTTATC 
ACAGCAATTG ACCGCTTTGA GCCGAAAAGG AAGTTTCGTC TTTCGACTTA TGGTTTGTTT 
TGGATTAGAC ATGCCATCAT ACGGTCAATG ACAACTTCAA ACTTCACTCG TGTCCCATTT 
GGACTTGAAT CGGTTAGAGT GGAGATATAT AAAGCAAAGA CTGAGCTATG GTTTGAGATG 
GGAAGACCTC CAACGGAGGA CGAGGTAGTT GAGAGACTCA AGATAACACC TGAGAGATAC 
CGTGAAGTAT TGAGGGCTGC AAAACCGGTT TTGTCACTAA ATTCAAAACA TTCGGTTACT 
CAAGAAGAAT TCATCAACGG AATCACAGAT GTTGATGGTG TTGGAGCTAA TAACAGCAGA 
CAGCCTGCTC TTCTCAGGCT CGCTCTCGAC GATGTGCTCG ATTCATTGAA GCCGAAAGAG 
AGTCTGGTTA TTCGACAGAG GTACGGTCTT GATGGAAAAG GCGACAGGAC TCTAGGAGAG 
ATAGCTGGAA ATCTCAACAT CTCTAGAGAA ATGGTTAGGA AACATGAAGT CAAGGCCTTG 
ATGAAGCTTA AGCATCAGGC CAGAGTTGAT TACCTTCGTC AATACATCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIGE ( NM_001343842.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330408.1 Arabidopsis thaliana sigma factor E (SIGE), mRNA.

Price & Availability↑

CloneID	OAb19398
Clone ID Related Accession (Same CDS sequence)	NM_001343841.1 , NM_001343842.1 , NM_122317.4
Accession Version	NM_001343842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	sigma factor E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGAGTTG TGTCTATTTC AAGTTCAGCT GCAAGATCTC CACTCGGGTT GGGCAACGAT 
CTTTTGACAC ATCGGTCTTC ACTTAAAAAG CCATCCATTG TTGCTTTTAA AGCCGATGAT 
TCCAGCAATT CAGCTTTGAT CATTCCTCCT CGTGAGCAAG TTTTGATTCC TGCTGAAAAG 
CATAATGAGA AGAAGAGAGA AGTAACGAGG AGAAAGCCTT GTAAAACCCC GAAAAAACCG 
CTTTCCTTGG AACATAATTC GGCTCCTTCG TGTTCGTTAG GAGTAGATTA CAATGAAGCT 
GCTGCAAGAC TTGAAAGCAT ATACAAGCTT AGTCCTGCAA CAACCACATT AGTCGAGGAT 
GATGTTGAAG ATGGCGGCTC TAAAGCCAAA GTTTCACGGA GGAGAAAAAG GAAAGAAAGT 
GGTGAGGAGA AGAAAGTGGT TGTGAGGAAT AATGTGAAGA AGGAAAAACG TATGAGTCTT 
GATAAACGGA TTGCTTTGAA GAGAAACGTT CAAGAGAAAC CAGTCGTTGC TTCTGTTGAT 
AAGAAAGTAA CAAAGAGGCA GCAAGAAGAG GAGAAGATCG AGAGGCTTGT GCGGGACTAT 
TCAGCTTCTA ATGATATAGT GAGCTTGGAC TGGAAAAAAA TGAAGATACC TCCTGTTCTT 
TCTTCTACTG AACATGCTTG GTTGTTCAAG TTGATGCAAC CTATGAAGGC TCTTCTTGAA 
GTGAAAGATG TATTGCAAAA GAGCTTGGGA AGAGAGCCTA GGGAAGCTGA AATAGCCGGG 
GAGATCAATA TGACTGCGGG TGAAGTAAAA AAGAAAATCG AAATAGGTAG AGCTGCAAGA 
AACAAACTTA TTAAGCACAA TCTCCGGCTT GTCTTGTTTG TTATGAACAA ATACTTTCAT 
GAGTTTACCA ACGGACCAAA ATTCCAAGAC CTTTGTCAAG CGGGTATGAG AGGGCTTATC 
ACAGCAATTG ACCGCTTTGA GCCGAAAAGG AAGTTTCGTC TTTCGACTTA TGGTTTGTTT 
TGGATTAGAC ATGCCATCAT ACGGTCAATG ACAACTTCAA ACTTCACTCG TGTCCCATTT 
GGACTTGAAT CGGTTAGAGT GGAGATATAT AAAGCAAAGA CTGAGCTATG GTTTGAGATG 
GGAAGACCTC CAACGGAGGA CGAGGTAGTT GAGAGACTCA AGATAACACC TGAGAGATAC 
CGTGAAGTAT TGAGGGCTGC AAAACCGGTT TTGTCACTAA ATTCAAAACA TTCGGTTACT 
CAAGAAGAAT TCATCAACGG AATCACAGAT GTTGATGGTG TTGGAGCTAA TAACAGCAGA 
CAGCCTGCTC TTCTCAGGCT CGCTCTCGAC GATGTGCTCG ATTCATTGAA GCCGAAAGAG 
AGTCTGGTTA TTCGACAGAG GTACGGTCTT GATGGAAAAG GCGACAGGAC TCTAGGAGAG 
ATAGCTGGAA ATCTCAACAT CTCTAGAGAA ATGGTTAGGA AACATGAAGT CAAGGCCTTG 
ATGAAGCTTA AGCATCAGGC CAGAGTTGAT TACCTTCGTC AATACATCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330408.1
CDS	295..1848
Translation	MGVVSISSSAARSPLGLGNDLLTHRSSLKKPSIVAFKADDSSNSALIIPPREQVLIPAEKHNEKKREVTRRKPCKTPKKPLSLEHNSAPSCSLGVDYNEAAARLESIYKLSPATTTLVEDDVEDGGSKAKVSRRRKRKESGEEKKVVVRNNVKKEKRMSLDKRIALKRNVQEKPVVASVDKKVTKRQQEEEKIERLVRDYSASNDIVSLDWKKMKIPPVLSSTEHAWLFKLMQPMKALLEVKDVLQKSLGREPREAEIAGEINMTAGEVKKKIEIGRAARNKLIKHNLRLVLFVMNKYFHEFTNGPKFQDLCQAGMRGLITAIDRFEPKRKFRLSTYGLFWIRHAIIRSMTTSNFTRVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLKITPERYREVLRAAKPVLSLNSKHSVTQEEFINGITDVDGVGANNSRQPALLRLALDDVLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRKHEVKALMKLKHQARVDYLRQYIV

Target ORF information:

RefSeq Version	NM_001343842.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana sigma factor E (SIGE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343842.1

ORF Insert Sequence:

ATGGGAGTTG TGTCTATTTC AAGTTCAGCT GCAAGATCTC CACTCGGGTT GGGCAACGAT 
CTTTTGACAC ATCGGTCTTC ACTTAAAAAG CCATCCATTG TTGCTTTTAA AGCCGATGAT 
TCCAGCAATT CAGCTTTGAT CATTCCTCCT CGTGAGCAAG TTTTGATTCC TGCTGAAAAG 
CATAATGAGA AGAAGAGAGA AGTAACGAGG AGAAAGCCTT GTAAAACCCC GAAAAAACCG 
CTTTCCTTGG AACATAATTC GGCTCCTTCG TGTTCGTTAG GAGTAGATTA CAATGAAGCT 
GCTGCAAGAC TTGAAAGCAT ATACAAGCTT AGTCCTGCAA CAACCACATT AGTCGAGGAT 
GATGTTGAAG ATGGCGGCTC TAAAGCCAAA GTTTCACGGA GGAGAAAAAG GAAAGAAAGT 
GGTGAGGAGA AGAAAGTGGT TGTGAGGAAT AATGTGAAGA AGGAAAAACG TATGAGTCTT 
GATAAACGGA TTGCTTTGAA GAGAAACGTT CAAGAGAAAC CAGTCGTTGC TTCTGTTGAT 
AAGAAAGTAA CAAAGAGGCA GCAAGAAGAG GAGAAGATCG AGAGGCTTGT GCGGGACTAT 
TCAGCTTCTA ATGATATAGT GAGCTTGGAC TGGAAAAAAA TGAAGATACC TCCTGTTCTT 
TCTTCTACTG AACATGCTTG GTTGTTCAAG TTGATGCAAC CTATGAAGGC TCTTCTTGAA 
GTGAAAGATG TATTGCAAAA GAGCTTGGGA AGAGAGCCTA GGGAAGCTGA AATAGCCGGG 
GAGATCAATA TGACTGCGGG TGAAGTAAAA AAGAAAATCG AAATAGGTAG AGCTGCAAGA 
AACAAACTTA TTAAGCACAA TCTCCGGCTT GTCTTGTTTG TTATGAACAA ATACTTTCAT 
GAGTTTACCA ACGGACCAAA ATTCCAAGAC CTTTGTCAAG CGGGTATGAG AGGGCTTATC 
ACAGCAATTG ACCGCTTTGA GCCGAAAAGG AAGTTTCGTC TTTCGACTTA TGGTTTGTTT 
TGGATTAGAC ATGCCATCAT ACGGTCAATG ACAACTTCAA ACTTCACTCG TGTCCCATTT 
GGACTTGAAT CGGTTAGAGT GGAGATATAT AAAGCAAAGA CTGAGCTATG GTTTGAGATG 
GGAAGACCTC CAACGGAGGA CGAGGTAGTT GAGAGACTCA AGATAACACC TGAGAGATAC 
CGTGAAGTAT TGAGGGCTGC AAAACCGGTT TTGTCACTAA ATTCAAAACA TTCGGTTACT 
CAAGAAGAAT TCATCAACGG AATCACAGAT GTTGATGGTG TTGGAGCTAA TAACAGCAGA 
CAGCCTGCTC TTCTCAGGCT CGCTCTCGAC GATGTGCTCG ATTCATTGAA GCCGAAAGAG 
AGTCTGGTTA TTCGACAGAG GTACGGTCTT GATGGAAAAG GCGACAGGAC TCTAGGAGAG 
ATAGCTGGAA ATCTCAACAT CTCTAGAGAA ATGGTTAGGA AACATGAAGT CAAGGCCTTG 
ATGAAGCTTA AGCATCAGGC CAGAGTTGAT TACCTTCGTC AATACATCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIGE ( NM_122317.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197800.1 Arabidopsis thaliana sigma factor E (SIGE), mRNA.

Price & Availability↑

CloneID	OAb19398
Clone ID Related Accession (Same CDS sequence)	NM_001343841.1 , NM_001343842.1 , NM_122317.4
Accession Version	NM_122317.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	sigma factor E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122317.3.

ATGGGAGTTG TGTCTATTTC AAGTTCAGCT GCAAGATCTC CACTCGGGTT GGGCAACGAT 
CTTTTGACAC ATCGGTCTTC ACTTAAAAAG CCATCCATTG TTGCTTTTAA AGCCGATGAT 
TCCAGCAATT CAGCTTTGAT CATTCCTCCT CGTGAGCAAG TTTTGATTCC TGCTGAAAAG 
CATAATGAGA AGAAGAGAGA AGTAACGAGG AGAAAGCCTT GTAAAACCCC GAAAAAACCG 
CTTTCCTTGG AACATAATTC GGCTCCTTCG TGTTCGTTAG GAGTAGATTA CAATGAAGCT 
GCTGCAAGAC TTGAAAGCAT ATACAAGCTT AGTCCTGCAA CAACCACATT AGTCGAGGAT 
GATGTTGAAG ATGGCGGCTC TAAAGCCAAA GTTTCACGGA GGAGAAAAAG GAAAGAAAGT 
GGTGAGGAGA AGAAAGTGGT TGTGAGGAAT AATGTGAAGA AGGAAAAACG TATGAGTCTT 
GATAAACGGA TTGCTTTGAA GAGAAACGTT CAAGAGAAAC CAGTCGTTGC TTCTGTTGAT 
AAGAAAGTAA CAAAGAGGCA GCAAGAAGAG GAGAAGATCG AGAGGCTTGT GCGGGACTAT 
TCAGCTTCTA ATGATATAGT GAGCTTGGAC TGGAAAAAAA TGAAGATACC TCCTGTTCTT 
TCTTCTACTG AACATGCTTG GTTGTTCAAG TTGATGCAAC CTATGAAGGC TCTTCTTGAA 
GTGAAAGATG TATTGCAAAA GAGCTTGGGA AGAGAGCCTA GGGAAGCTGA AATAGCCGGG 
GAGATCAATA TGACTGCGGG TGAAGTAAAA AAGAAAATCG AAATAGGTAG AGCTGCAAGA 
AACAAACTTA TTAAGCACAA TCTCCGGCTT GTCTTGTTTG TTATGAACAA ATACTTTCAT 
GAGTTTACCA ACGGACCAAA ATTCCAAGAC CTTTGTCAAG CGGGTATGAG AGGGCTTATC 
ACAGCAATTG ACCGCTTTGA GCCGAAAAGG AAGTTTCGTC TTTCGACTTA TGGTTTGTTT 
TGGATTAGAC ATGCCATCAT ACGGTCAATG ACAACTTCAA ACTTCACTCG TGTCCCATTT 
GGACTTGAAT CGGTTAGAGT GGAGATATAT AAAGCAAAGA CTGAGCTATG GTTTGAGATG 
GGAAGACCTC CAACGGAGGA CGAGGTAGTT GAGAGACTCA AGATAACACC TGAGAGATAC 
CGTGAAGTAT TGAGGGCTGC AAAACCGGTT TTGTCACTAA ATTCAAAACA TTCGGTTACT 
CAAGAAGAAT TCATCAACGG AATCACAGAT GTTGATGGTG TTGGAGCTAA TAACAGCAGA 
CAGCCTGCTC TTCTCAGGCT CGCTCTCGAC GATGTGCTCG ATTCATTGAA GCCGAAAGAG 
AGTCTGGTTA TTCGACAGAG GTACGGTCTT GATGGAAAAG GCGACAGGAC TCTAGGAGAG 
ATAGCTGGAA ATCTCAACAT CTCTAGAGAA ATGGTTAGGA AACATGAAGT CAAGGCCTTG 
ATGAAGCTTA AGCATCAGGC CAGAGTTGAT TACCTTCGTC AATACATCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197800.1
CDS	222..1775
Translation	MGVVSISSSAARSPLGLGNDLLTHRSSLKKPSIVAFKADDSSNSALIIPPREQVLIPAEKHNEKKREVTRRKPCKTPKKPLSLEHNSAPSCSLGVDYNEAAARLESIYKLSPATTTLVEDDVEDGGSKAKVSRRRKRKESGEEKKVVVRNNVKKEKRMSLDKRIALKRNVQEKPVVASVDKKVTKRQQEEEKIERLVRDYSASNDIVSLDWKKMKIPPVLSSTEHAWLFKLMQPMKALLEVKDVLQKSLGREPREAEIAGEINMTAGEVKKKIEIGRAARNKLIKHNLRLVLFVMNKYFHEFTNGPKFQDLCQAGMRGLITAIDRFEPKRKFRLSTYGLFWIRHAIIRSMTTSNFTRVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLKITPERYREVLRAAKPVLSLNSKHSVTQEEFINGITDVDGVGANNSRQPALLRLALDDVLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRKHEVKALMKLKHQARVDYLRQYIV

Target ORF information:

RefSeq Version	NM_122317.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana sigma factor E (SIGE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122317.4

ORF Insert Sequence:

ATGGGAGTTG TGTCTATTTC AAGTTCAGCT GCAAGATCTC CACTCGGGTT GGGCAACGAT 
CTTTTGACAC ATCGGTCTTC ACTTAAAAAG CCATCCATTG TTGCTTTTAA AGCCGATGAT 
TCCAGCAATT CAGCTTTGAT CATTCCTCCT CGTGAGCAAG TTTTGATTCC TGCTGAAAAG 
CATAATGAGA AGAAGAGAGA AGTAACGAGG AGAAAGCCTT GTAAAACCCC GAAAAAACCG 
CTTTCCTTGG AACATAATTC GGCTCCTTCG TGTTCGTTAG GAGTAGATTA CAATGAAGCT 
GCTGCAAGAC TTGAAAGCAT ATACAAGCTT AGTCCTGCAA CAACCACATT AGTCGAGGAT 
GATGTTGAAG ATGGCGGCTC TAAAGCCAAA GTTTCACGGA GGAGAAAAAG GAAAGAAAGT 
GGTGAGGAGA AGAAAGTGGT TGTGAGGAAT AATGTGAAGA AGGAAAAACG TATGAGTCTT 
GATAAACGGA TTGCTTTGAA GAGAAACGTT CAAGAGAAAC CAGTCGTTGC TTCTGTTGAT 
AAGAAAGTAA CAAAGAGGCA GCAAGAAGAG GAGAAGATCG AGAGGCTTGT GCGGGACTAT 
TCAGCTTCTA ATGATATAGT GAGCTTGGAC TGGAAAAAAA TGAAGATACC TCCTGTTCTT 
TCTTCTACTG AACATGCTTG GTTGTTCAAG TTGATGCAAC CTATGAAGGC TCTTCTTGAA 
GTGAAAGATG TATTGCAAAA GAGCTTGGGA AGAGAGCCTA GGGAAGCTGA AATAGCCGGG 
GAGATCAATA TGACTGCGGG TGAAGTAAAA AAGAAAATCG AAATAGGTAG AGCTGCAAGA 
AACAAACTTA TTAAGCACAA TCTCCGGCTT GTCTTGTTTG TTATGAACAA ATACTTTCAT 
GAGTTTACCA ACGGACCAAA ATTCCAAGAC CTTTGTCAAG CGGGTATGAG AGGGCTTATC 
ACAGCAATTG ACCGCTTTGA GCCGAAAAGG AAGTTTCGTC TTTCGACTTA TGGTTTGTTT 
TGGATTAGAC ATGCCATCAT ACGGTCAATG ACAACTTCAA ACTTCACTCG TGTCCCATTT 
GGACTTGAAT CGGTTAGAGT GGAGATATAT AAAGCAAAGA CTGAGCTATG GTTTGAGATG 
GGAAGACCTC CAACGGAGGA CGAGGTAGTT GAGAGACTCA AGATAACACC TGAGAGATAC 
CGTGAAGTAT TGAGGGCTGC AAAACCGGTT TTGTCACTAA ATTCAAAACA TTCGGTTACT 
CAAGAAGAAT TCATCAACGG AATCACAGAT GTTGATGGTG TTGGAGCTAA TAACAGCAGA 
CAGCCTGCTC TTCTCAGGCT CGCTCTCGAC GATGTGCTCG ATTCATTGAA GCCGAAAGAG 
AGTCTGGTTA TTCGACAGAG GTACGGTCTT GATGGAAAAG GCGACAGGAC TCTAGGAGAG 
ATAGCTGGAA ATCTCAACAT CTCTAGAGAA ATGGTTAGGA AACATGAAGT CAAGGCCTTG 
ATGAAGCTTA AGCATCAGGC CAGAGTTGAT TACCTTCGTC AATACATCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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SIGE cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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