EMB1030 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMB1030
Entrez Gene ID	832343
Full Name	Alanyl-tRNA synthetase, class IIc
Synonyms	EMB263,EMB86,EMBRYO DEFECTIVE 1030,EMBRYO DEFECTIVE 263,EMBRYO DEFECTIVE 86
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001343771.1	NP_001330952.1	Alanyl-tRNA synthetase, class IIc
NM_147905.3	NP_680210.2	Alanyl-tRNA synthetase, class IIc

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EMB1030 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMB1030 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19268	NM_147905.3 Latest version!	Arabidopsis thaliana Alanyl-tRNA synthetase, class IIc (EMB1030), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb41121	NM_001343771.1 Latest version!	Arabidopsis thaliana Alanyl-tRNA synthetase, class IIc (EMB1030), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMB1030 ( NM_147905.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_680210.2 Arabidopsis thaliana Alanyl-tRNA synthetase, class IIc (EMB1030), mRNA.

Price & Availability↑

CloneID	OAb19268
Clone ID Related Accession (Same CDS sequence)	NM_147905.3
Accession Version	NM_147905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Alanyl-tRNA synthetase, class IIc
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_147905.2.

ATGAATTTCT CCAGAGTAAA CCTCTTCGAT TTTCCTCTTA GACCAATTTT GCTTTCGCAT 
CCTTCTTCTA TTTTCGTTTC TACACGTTTT GTTACCAGAA CCTCTGCAGG TGTTTCTCCT 
TCTATCTTAC TTCCCAGATC AACTCAGTCT CCTCAGATTA TTGCTAAGAG CTCATCAGTA 
TCAGTACAGC CAGTGTCTGA GGATGCTAAG GAGGATTATC AGTCCAAAGA TGTTAGTGGA 
GATTCAATAC GGCGGCGTTT TCTTGAATTC TTTGCTTCTC GTGGTCATAA GGTGCTTCCA 
AGTTCGTCTC TTGTACCAGA AGATCCTACC GTCTTGCTAA CAATTGCAGG AATGCTTCAG 
TTTAAGCCTA TTTTCCTTGG AAAGGTACCT AGAGAGGTTC CTTGTGCAAC CACTGCGCAA 
AGGTGTATAC GTACGAATGA TTTGGAGAAT GTTGGGAAAA CGGCTAGGCA CCATACTTTC 
TTTGAGATGC TTGGGAACTT TAGCTTTGGT GATTACTTCA AGAAAGAAGC GATAAAATGG 
GCATGGGAGC TTTCAACTAT TGAGTTTGGG CTACCAGCTA ATAGAGTTTG GGTTAGTATA 
TATGAAGACG ATGATGAAGC TTTTGAAATC TGGAAGAATG AAGTTGGTGT TTCTGTTGAG 
CGGATAAAGA GAATGGGTGA AGCTGACAAC TTTTGGACTA GTGGACCAAC TGGTCCTTGT 
GGTCCATGCT CTGAGTTGTA CTATGACTTC TATCCTGAGA GAGGTTATGA TGAAGATGTT 
GATCTTGGGG ATGATACCAG ATTTATTGAG TTCTATAATT TGGTTTTCAT GCAGTATAAC 
AAGACGGAAG ATGGATTGCT TGAGCCCTTG AAACAGAAGA ATATAGATAC TGGTCTTGGT 
TTGGAACGTA TAGCTCAAAT CCTTCAGAAG GTTCCAAACA ACTACGAGAC AGATTTGATA 
TATCCAATCA TTGCAAAGAT CTCAGAGTTG GCGAATATCT CATATGACTC TGCAAATGAC 
AAGGCAAAGA CAAGTTTAAA AGTGATTGCA GATCACATGC GGGCAGTTGT CTATCTCATA 
TCAGATGGTG TTTCTCCTTC AAATATTGGC AGAGGTTATG TGGTTAGGAG GCTAATAAGA 
AGAGCAGTTC GGAAGGGGAA GTCTCTCGGA ATAAATGGGG ATATGAATGG TAATCTAAAG 
GGAGCGTTTT TGCCAGCGGT TGCTGAAAAG GTGATAGAGT TGAGCACTTA TATTGATTCA 
GATGTAAAAC TAAAGGCCTC ACGCATCATT GAGGAGATTA GGCAAGAAGA ACTTCACTTT 
AAGAAAACTC TGGAAAGAGG AGAAAAGTTA CTTGACCAAA AGCTTAACGA TGCATTGTCA 
ATTGCTGATA AAACTAAGGA TACGCCTTAT CTGGATGGAA AAGATGCGTT TCTTCTTTAT 
GACACATTTG GCTTTCCTGT GGAGATAACT GCAGAAGTTG CTGAAGAACG TGGAGTCAGT 
ATAGATATGA ATGGTTTTGA AGTGGAAATG GAGAATCAAA GACGTCAATC TCAAGCTGCT 
CACAATGTTG TAAAACTGAC AGTTGAAGAC GATGCTGACA TGACGAAAAA TATTGCAGAC 
ACTGAGTTCC TTGGATATGA CAGTCTCTCT GCTCGTGCTG TTGTGAAAAG TCTTTTGGTG 
AATGGGAAGC CTGTGATAAG GGTTTCTGAA GGCAGTGAAG TAGAGGTTCT GCTGGACAGA 
ACTCCGTTCT ATGCTGAATC AGGAGGTCAA ATTGCAGATC ATGGTTTTCT TTATGTTAGC 
AGTGATGGGA ACCAAGAGAA AGCTGTTGTT GAGGTAAGTG ATGTGCAGAA GTCTCTTAAA 
ATTTTTGTTC ACAAGGGCAC TGTAAAAAGT GGAGCTCTAG AAGTTGGCAA GGAGGTGGAA 
GCAGCAGTAG ATGCAGACTT GAGGCAACGA GCGAAGGTTC ACCATACCGC CACTCATTTG 
CTCCAATCGG CACTTAAAAA AGTAGTAGGA CAAGAAACAT CACAGGCTGG TTCATTAGTA 
GCTTTTGACC GCCTCAGATT CGATTTCAAT TTTAATCGGT CCCTGCATGA TAATGAGCTT 
GAGGAAATCG AATGCCTGAT CAATAGGTGG ATTGGGGATG CTACACGTCT TGAAACAAAA 
GTCCTTCCTC TTGCTGATGC AAAACGTGCT GGAGCCATCG CAATGTTTGG GGAAAAATAT 
GATGAAAACG AGGTTCGTGT AGTAGAAGTT CCTGGTGTCT CCATGGAACT TTGTGGTGGC 
ACTCATGTTG GCAATACTGC AGAAATACGA GCCTTCAAGA TTATCTCAGA ACAGGGCATT 
GCATCTGGAA TCCGGCGTAT AGAAGCGGTT GCAGGTGAAG CATTCATTGA ATACATAAAC 
TCACGGGATT CTCAAATGAC ACGTCTATGC TCGACTCTCA AGGTGAAAGC AGAGGATGTT 
ACAAACAGAG TGGAGAATCT TCTAGAGGAA CTACGTGCTG CTAGAAAAGA AGCCTCCGAC 
TTGCGTTCAA AAGCAGCTGT CTATAAAGCA TCTGTCATAT CGAACAAAGC ATTTACTGTA 
GGAACTTCAC AGACTATAAG AGTGCTCGTT GAGTCGATGG ATGACACCGA TGCTGACTCA 
TTAAAGAGTG CAGCTGAGCA TTTGATAAGC ACATTGGAAG ATCCAGTCGC TGTGGTACTA 
GGATCATCTC CAGAAAAAGA CAAGGTTAGT TTAGTTGCTG CATTTAGTCC TGGAGTAGTC 
TCCCTAGGTG TTCAAGCAGG GAAATTCATT GGCCCCATAG CTAAGCTGTG TGGCGGAGGA 
GGTGGTGGAA AGCCCAATTT TGCTCAGGCA GGCGGCAGAA AGCCTGAAAA TCTCCCAAGT 
GCCTTAGAGA AAGCTCGGGA AGATCTCGTG GCAACTCTAT CCGAAAAGCT AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_680210.2
CDS	119..3055
Translation	MNFSRVNLFDFPLRPILLSHPSSIFVSTRFVTRTSAGVSPSILLPRSTQSPQIIAKSSSVSVQPVSEDAKEDYQSKDVSGDSIRRRFLEFFASRGHKVLPSSSLVPEDPTVLLTIAGMLQFKPIFLGKVPREVPCATTAQRCIRTNDLENVGKTARHHTFFEMLGNFSFGDYFKKEAIKWAWELSTIEFGLPANRVWVSIYEDDDEAFEIWKNEVGVSVERIKRMGEADNFWTSGPTGPCGPCSELYYDFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQKNIDTGLGLERIAQILQKVPNNYETDLIYPIIAKISELANISYDSANDKAKTSLKVIADHMRAVVYLISDGVSPSNIGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIELSTYIDSDVKLKASRIIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDGKDAFLLYDTFGFPVEITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHNVVKLTVEDDADMTKNIADTEFLGYDSLSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQIADHGFLYVSSDGNQEKAVVEVSDVQKSLKIFVHKGTVKSGALEVGKEVEAAVDADLRQRAKVHHTATHLLQSALKKVVGQETSQAGSLVAFDRLRFDFNFNRSLHDNELEEIECLINRWIGDATRLETKVLPLADAKRAGAIAMFGEKYDENEVRVVEVPGVSMELCGGTHVGNTAEIRAFKIISEQGIASGIRRIEAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLEELRAARKEASDLRSKAAVYKASVISNKAFTVGTSQTIRVLVESMDDTDADSLKSAAEHLISTLEDPVAVVLGSSPEKDKVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGGRKPENLPSALEKAREDLVATLSEKLG

Target ORF information:

RefSeq Version	NM_147905.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Alanyl-tRNA synthetase, class IIc (EMB1030), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147905.3

ORF Insert Sequence:

ATGAATTTCT CCAGAGTAAA CCTCTTCGAT TTTCCTCTTA GACCAATTTT GCTTTCGCAT 
CCTTCTTCTA TTTTCGTTTC TACACGTTTT GTTACCAGAA CCTCTGCAGG TGTTTCTCCT 
TCTATCTTAC TTCCCAGATC AACTCAGTCT CCTCAGATTA TTGCTAAGAG CTCATCAGTA 
TCAGTACAGC CAGTGTCTGA GGATGCTAAG GAGGATTATC AGTCCAAAGA TGTTAGTGGA 
GATTCAATAC GGCGGCGTTT TCTTGAATTC TTTGCTTCTC GTGGTCATAA GGTGCTTCCA 
AGTTCGTCTC TTGTACCAGA AGATCCTACC GTCTTGCTAA CAATTGCAGG AATGCTTCAG 
TTTAAGCCTA TTTTCCTTGG AAAGGTACCT AGAGAGGTTC CTTGTGCAAC CACTGCGCAA 
AGGTGTATAC GTACGAATGA TTTGGAGAAT GTTGGGAAAA CGGCTAGGCA CCATACTTTC 
TTTGAGATGC TTGGGAACTT TAGCTTTGGT GATTACTTCA AGAAAGAAGC GATAAAATGG 
GCATGGGAGC TTTCAACTAT TGAGTTTGGG CTACCAGCTA ATAGAGTTTG GGTTAGTATA 
TATGAAGACG ATGATGAAGC TTTTGAAATC TGGAAGAATG AAGTTGGTGT TTCTGTTGAG 
CGGATAAAGA GAATGGGTGA AGCTGACAAC TTTTGGACTA GTGGACCAAC TGGTCCTTGT 
GGTCCATGCT CTGAGTTGTA CTATGACTTC TATCCTGAGA GAGGTTATGA TGAAGATGTT 
GATCTTGGGG ATGATACCAG ATTTATTGAG TTCTATAATT TGGTTTTCAT GCAGTATAAC 
AAGACGGAAG ATGGATTGCT TGAGCCCTTG AAACAGAAGA ATATAGATAC TGGTCTTGGT 
TTGGAACGTA TAGCTCAAAT CCTTCAGAAG GTTCCAAACA ACTACGAGAC AGATTTGATA 
TATCCAATCA TTGCAAAGAT CTCAGAGTTG GCGAATATCT CATATGACTC TGCAAATGAC 
AAGGCAAAGA CAAGTTTAAA AGTGATTGCA GATCACATGC GGGCAGTTGT CTATCTCATA 
TCAGATGGTG TTTCTCCTTC AAATATTGGC AGAGGTTATG TGGTTAGGAG GCTAATAAGA 
AGAGCAGTTC GGAAGGGGAA GTCTCTCGGA ATAAATGGGG ATATGAATGG TAATCTAAAG 
GGAGCGTTTT TGCCAGCGGT TGCTGAAAAG GTGATAGAGT TGAGCACTTA TATTGATTCA 
GATGTAAAAC TAAAGGCCTC ACGCATCATT GAGGAGATTA GGCAAGAAGA ACTTCACTTT 
AAGAAAACTC TGGAAAGAGG AGAAAAGTTA CTTGACCAAA AGCTTAACGA TGCATTGTCA 
ATTGCTGATA AAACTAAGGA TACGCCTTAT CTGGATGGAA AAGATGCGTT TCTTCTTTAT 
GACACATTTG GCTTTCCTGT GGAGATAACT GCAGAAGTTG CTGAAGAACG TGGAGTCAGT 
ATAGATATGA ATGGTTTTGA AGTGGAAATG GAGAATCAAA GACGTCAATC TCAAGCTGCT 
CACAATGTTG TAAAACTGAC AGTTGAAGAC GATGCTGACA TGACGAAAAA TATTGCAGAC 
ACTGAGTTCC TTGGATATGA CAGTCTCTCT GCTCGTGCTG TTGTGAAAAG TCTTTTGGTG 
AATGGGAAGC CTGTGATAAG GGTTTCTGAA GGCAGTGAAG TAGAGGTTCT GCTGGACAGA 
ACTCCGTTCT ATGCTGAATC AGGAGGTCAA ATTGCAGATC ATGGTTTTCT TTATGTTAGC 
AGTGATGGGA ACCAAGAGAA AGCTGTTGTT GAGGTAAGTG ATGTGCAGAA GTCTCTTAAA 
ATTTTTGTTC ACAAGGGCAC TGTAAAAAGT GGAGCTCTAG AAGTTGGCAA GGAGGTGGAA 
GCAGCAGTAG ATGCAGACTT GAGGCAACGA GCGAAGGTTC ACCATACCGC CACTCATTTG 
CTCCAATCGG CACTTAAAAA AGTAGTAGGA CAAGAAACAT CACAGGCTGG TTCATTAGTA 
GCTTTTGACC GCCTCAGATT CGATTTCAAT TTTAATCGGT CCCTGCATGA TAATGAGCTT 
GAGGAAATCG AATGCCTGAT CAATAGGTGG ATTGGGGATG CTACACGTCT TGAAACAAAA 
GTCCTTCCTC TTGCTGATGC AAAACGTGCT GGAGCCATCG CAATGTTTGG GGAAAAATAT 
GATGAAAACG AGGTTCGTGT AGTAGAAGTT CCTGGTGTCT CCATGGAACT TTGTGGTGGC 
ACTCATGTTG GCAATACTGC AGAAATACGA GCCTTCAAGA TTATCTCAGA ACAGGGCATT 
GCATCTGGAA TCCGGCGTAT AGAAGCGGTT GCAGGTGAAG CATTCATTGA ATACATAAAC 
TCACGGGATT CTCAAATGAC ACGTCTATGC TCGACTCTCA AGGTGAAAGC AGAGGATGTT 
ACAAACAGAG TGGAGAATCT TCTAGAGGAA CTACGTGCTG CTAGAAAAGA AGCCTCCGAC 
TTGCGTTCAA AAGCAGCTGT CTATAAAGCA TCTGTCATAT CGAACAAAGC ATTTACTGTA 
GGAACTTCAC AGACTATAAG AGTGCTCGTT GAGTCGATGG ATGACACCGA TGCTGACTCA 
TTAAAGAGTG CAGCTGAGCA TTTGATAAGC ACATTGGAAG ATCCAGTCGC TGTGGTACTA 
GGATCATCTC CAGAAAAAGA CAAGGTTAGT TTAGTTGCTG CATTTAGTCC TGGAGTAGTC 
TCCCTAGGTG TTCAAGCAGG GAAATTCATT GGCCCCATAG CTAAGCTGTG TGGCGGAGGA 
GGTGGTGGAA AGCCCAATTT TGCTCAGGCA GGCGGCAGAA AGCCTGAAAA TCTCCCAAGT 
GCCTTAGAGA AAGCTCGGGA AGATCTCGTG GCAACTCTAT CCGAAAAGCT AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB1030 ( NM_001343771.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330952.1 Arabidopsis thaliana Alanyl-tRNA synthetase, class IIc (EMB1030), mRNA.

Price & Availability↑

CloneID	OAb41121
Clone ID Related Accession (Same CDS sequence)	NM_001343771.1
Accession Version	NM_001343771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Alanyl-tRNA synthetase, class IIc
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGTACTTC ATTTTTCAAG AAGAAGAAGT TTGCTCATTC ACTTTTATTC TCTCGTTTTC 
ATCTTCTTTA TTAAATTCTT TGTTTTTATT AAAAATCGAA GAAGGGGTCT CTCGAGTCTC 
CTCCAATGGG AGAAAAAACA ATCTCTAACC ACTAATGAAG GTTTTGTTAC CAGAACCTCT 
GCAGGTGTTT CTCCTTCTAT CTTACTTCCC AGATCAACTC AGTCTCCTCA GATTATTGCT 
AAGAGCTCAT CAGTATCAGT ACAGCCAGTG TCTGAGGATG CTAAGGAGGA TTATCAGTCC 
AAAGATGTTA GTGGAGATTC AATACGGCGG CGTTTTCTTG AATTCTTTGC TTCTCGTGGT 
CATAAGGTGC TTCCAAGTTC GTCTCTTGTA CCAGAAGATC CTACCGTCTT GCTAACAATT 
GCAGGAATGC TTCAGTTTAA GCCTATTTTC CTTGGAAAGG TACCTAGAGA GGTTCCTTGT 
GCAACCACTG CGCAAAGGTG TATACGTACG AATGATTTGG AGAATGTTGG GAAAACGGCT 
AGGCACCATA CTTTCTTTGA GATGCTTGGG AACTTTAGCT TTGGTGATTA CTTCAAGAAA 
GAAGCGATAA AATGGGCATG GGAGCTTTCA ACTATTGAGT TTGGGCTACC AGCTAATAGA 
GTTTGGGTTA GTATATATGA AGACGATGAT GAAGCTTTTG AAATCTGGAA GAATGAAGTT 
GGTGTTTCTG TTGAGCGGAT AAAGAGAATG GGTGAAGCTG ACAACTTTTG GACTAGTGGA 
CCAACTGGTC CTTGTGGTCC ATGCTCTGAG TTGTACTATG ACTTCTATCC TGAGAGAGGT 
TATGATGAAG ATGTTGATCT TGGGGATGAT ACCAGATTTA TTGAGTTCTA TAATTTGGTT 
TTCATGCAGT ATAACAAGAC GGAAGATGGA TTGCTTGAGC CCTTGAAACA GAAGAATATA 
GATACTGGTC TTGGTTTGGA ACGTATAGCT CAAATCCTTC AGAAGGTTCC AAACAACTAC 
GAGACAGATT TGATATATCC AATCATTGCA AAGATCTCAG AGTTGGCGAA TATCTCATAT 
GACTCTGCAA ATGACAAGGC AAAGACAAGT TTAAAAGTGA TTGCAGATCA CATGCGGGCA 
GTTGTCTATC TCATATCAGA TGGTGTTTCT CCTTCAAATA TTGGCAGAGG TTATGTGGTT 
AGGAGGCTAA TAAGAAGAGC AGTTCGGAAG GGGAAGTCTC TCGGAATAAA TGGGGATATG 
AATGGTAATC TAAAGGGAGC GTTTTTGCCA GCGGTTGCTG AAAAGGTGAT AGAGTTGAGC 
ACTTATATTG ATTCAGATGT AAAACTAAAG GCCTCACGCA TCATTGAGGA GATTAGGCAA 
GAAGAACTTC ACTTTAAGAA AACTCTGGAA AGAGGAGAAA AGTTACTTGA CCAAAAGCTT 
AACGATGCAT TGTCAATTGC TGATAAAACT AAGGATACGC CTTATCTGGA TGGAAAAGAT 
GCGTTTCTTC TTTATGACAC ATTTGGCTTT CCTGTGGAGA TAACTGCAGA AGTTGCTGAA 
GAACGTGGAG TCAGTATAGA TATGAATGGT TTTGAAGTGG AAATGGAGAA TCAAAGACGT 
CAATCTCAAG CTGCTCACAA TGTTGTAAAA CTGACAGTTG AAGACGATGC TGACATGACG 
AAAAATATTG CAGACACTGA GTTCCTTGGA TATGACAGTC TCTCTGCTCG TGCTGTTGTG 
AAAAGTCTTT TGGTGAATGG GAAGCCTGTG ATAAGGGTTT CTGAAGGCAG TGAAGTAGAG 
GTTCTGCTGG ACAGAACTCC GTTCTATGCT GAATCAGGAG GTCAAATTGC AGATCATGGT 
TTTCTTTATG TTAGCAGTGA TGGGAACCAA GAGAAAGCTG TTGTTGAGGT AAGTGATGTG 
CAGAAGTCTC TTAAAATTTT TGTTCACAAG GGCACTGTAA AAAGTGGAGC TCTAGAAGTT 
GGCAAGGAGG TGGAAGCAGC AGTAGATGCA GACTTGAGGC AACGAGCGAA GGTTCACCAT 
ACCGCCACTC ATTTGCTCCA ATCGGCACTT AAAAAAGTAG TAGGACAAGA AACATCACAG 
GCTGGTTCAT TAGTAGCTTT TGACCGCCTC AGATTCGATT TCAATTTTAA TCGGTCCCTG 
CATGATAATG AGCTTGAGGA AATCGAATGC CTGATCAATA GGTGGATTGG GGATGCTACA 
CGTCTTGAAA CAAAAGTCCT TCCTCTTGCT GATGCAAAAC GTGCTGGAGC CATCGCAATG 
TTTGGGGAAA AATATGATGA AAACGAGGTT CGTGTAGTAG AAGTTCCTGG TGTCTCCATG 
GAACTTTGTG GTGGCACTCA TGTTGGCAAT ACTGCAGAAA TACGAGCCTT CAAGATTATC 
TCAGAACAGG GCATTGCATC TGGAATCCGG CGTATAGAAG CGGTTGCAGG TGAAGCATTC 
ATTGAATACA TAAACTCACG GGATTCTCAA ATGACACGTC TATGCTCGAC TCTCAAGGTG 
AAAGCAGAGG ATGTTACAAA CAGAGTGGAG AATCTTCTAG AGGAACTACG TGCTGCTAGA 
AAAGAAGCCT CCGACTTGCG TTCAAAAGCA GCTGTCTATA AAGCATCTGT CATATCGAAC 
AAAGCATTTA CTGTAGGAAC TTCACAGACT ATAAGAGTGC TCGTTGAGTC GATGGATGAC 
ACCGATGCTG ACTCATTAAA GAGTGCAGCT GAGCATTTGA TAAGCACATT GGAAGATCCA 
GTCGCTGTGG TACTAGGATC ATCTCCAGAA AAAGACAAGG TTAGTTTAGT TGCTGCATTT 
AGTCCTGGAG TAGTCTCCCT AGGTGTTCAA GCAGGGAAAT TCATTGGCCC CATAGCTAAG 
CTGTGTGGCG GAGGAGGTGG TGGAAAGCCC AATTTTGCTC AGGCAGGCGG CAGAAAGCCT 
GAAAATCTCC CAAGTGCCTT AGAGAAAGCT CGGGAAGATC TCGTGGCAAC TCTATCCGAA 
AAGCTAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330952.1
CDS	6..3017
Translation	MVLHFSRRRSLLIHFYSLVFIFFIKFFVFIKNRRRGLSSLLQWEKKQSLTTNEGFVTRTSAGVSPSILLPRSTQSPQIIAKSSSVSVQPVSEDAKEDYQSKDVSGDSIRRRFLEFFASRGHKVLPSSSLVPEDPTVLLTIAGMLQFKPIFLGKVPREVPCATTAQRCIRTNDLENVGKTARHHTFFEMLGNFSFGDYFKKEAIKWAWELSTIEFGLPANRVWVSIYEDDDEAFEIWKNEVGVSVERIKRMGEADNFWTSGPTGPCGPCSELYYDFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQKNIDTGLGLERIAQILQKVPNNYETDLIYPIIAKISELANISYDSANDKAKTSLKVIADHMRAVVYLISDGVSPSNIGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIELSTYIDSDVKLKASRIIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDGKDAFLLYDTFGFPVEITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHNVVKLTVEDDADMTKNIADTEFLGYDSLSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQIADHGFLYVSSDGNQEKAVVEVSDVQKSLKIFVHKGTVKSGALEVGKEVEAAVDADLRQRAKVHHTATHLLQSALKKVVGQETSQAGSLVAFDRLRFDFNFNRSLHDNELEEIECLINRWIGDATRLETKVLPLADAKRAGAIAMFGEKYDENEVRVVEVPGVSMELCGGTHVGNTAEIRAFKIISEQGIASGIRRIEAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLEELRAARKEASDLRSKAAVYKASVISNKAFTVGTSQTIRVLVESMDDTDADSLKSAAEHLISTLEDPVAVVLGSSPEKDKVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGGRKPENLPSALEKAREDLVATLSEKLG

Target ORF information:

RefSeq Version	NM_001343771.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Alanyl-tRNA synthetase, class IIc (EMB1030), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343771.1

ORF Insert Sequence:

ATGGTACTTC ATTTTTCAAG AAGAAGAAGT TTGCTCATTC ACTTTTATTC TCTCGTTTTC 
ATCTTCTTTA TTAAATTCTT TGTTTTTATT AAAAATCGAA GAAGGGGTCT CTCGAGTCTC 
CTCCAATGGG AGAAAAAACA ATCTCTAACC ACTAATGAAG GTTTTGTTAC CAGAACCTCT 
GCAGGTGTTT CTCCTTCTAT CTTACTTCCC AGATCAACTC AGTCTCCTCA GATTATTGCT 
AAGAGCTCAT CAGTATCAGT ACAGCCAGTG TCTGAGGATG CTAAGGAGGA TTATCAGTCC 
AAAGATGTTA GTGGAGATTC AATACGGCGG CGTTTTCTTG AATTCTTTGC TTCTCGTGGT 
CATAAGGTGC TTCCAAGTTC GTCTCTTGTA CCAGAAGATC CTACCGTCTT GCTAACAATT 
GCAGGAATGC TTCAGTTTAA GCCTATTTTC CTTGGAAAGG TACCTAGAGA GGTTCCTTGT 
GCAACCACTG CGCAAAGGTG TATACGTACG AATGATTTGG AGAATGTTGG GAAAACGGCT 
AGGCACCATA CTTTCTTTGA GATGCTTGGG AACTTTAGCT TTGGTGATTA CTTCAAGAAA 
GAAGCGATAA AATGGGCATG GGAGCTTTCA ACTATTGAGT TTGGGCTACC AGCTAATAGA 
GTTTGGGTTA GTATATATGA AGACGATGAT GAAGCTTTTG AAATCTGGAA GAATGAAGTT 
GGTGTTTCTG TTGAGCGGAT AAAGAGAATG GGTGAAGCTG ACAACTTTTG GACTAGTGGA 
CCAACTGGTC CTTGTGGTCC ATGCTCTGAG TTGTACTATG ACTTCTATCC TGAGAGAGGT 
TATGATGAAG ATGTTGATCT TGGGGATGAT ACCAGATTTA TTGAGTTCTA TAATTTGGTT 
TTCATGCAGT ATAACAAGAC GGAAGATGGA TTGCTTGAGC CCTTGAAACA GAAGAATATA 
GATACTGGTC TTGGTTTGGA ACGTATAGCT CAAATCCTTC AGAAGGTTCC AAACAACTAC 
GAGACAGATT TGATATATCC AATCATTGCA AAGATCTCAG AGTTGGCGAA TATCTCATAT 
GACTCTGCAA ATGACAAGGC AAAGACAAGT TTAAAAGTGA TTGCAGATCA CATGCGGGCA 
GTTGTCTATC TCATATCAGA TGGTGTTTCT CCTTCAAATA TTGGCAGAGG TTATGTGGTT 
AGGAGGCTAA TAAGAAGAGC AGTTCGGAAG GGGAAGTCTC TCGGAATAAA TGGGGATATG 
AATGGTAATC TAAAGGGAGC GTTTTTGCCA GCGGTTGCTG AAAAGGTGAT AGAGTTGAGC 
ACTTATATTG ATTCAGATGT AAAACTAAAG GCCTCACGCA TCATTGAGGA GATTAGGCAA 
GAAGAACTTC ACTTTAAGAA AACTCTGGAA AGAGGAGAAA AGTTACTTGA CCAAAAGCTT 
AACGATGCAT TGTCAATTGC TGATAAAACT AAGGATACGC CTTATCTGGA TGGAAAAGAT 
GCGTTTCTTC TTTATGACAC ATTTGGCTTT CCTGTGGAGA TAACTGCAGA AGTTGCTGAA 
GAACGTGGAG TCAGTATAGA TATGAATGGT TTTGAAGTGG AAATGGAGAA TCAAAGACGT 
CAATCTCAAG CTGCTCACAA TGTTGTAAAA CTGACAGTTG AAGACGATGC TGACATGACG 
AAAAATATTG CAGACACTGA GTTCCTTGGA TATGACAGTC TCTCTGCTCG TGCTGTTGTG 
AAAAGTCTTT TGGTGAATGG GAAGCCTGTG ATAAGGGTTT CTGAAGGCAG TGAAGTAGAG 
GTTCTGCTGG ACAGAACTCC GTTCTATGCT GAATCAGGAG GTCAAATTGC AGATCATGGT 
TTTCTTTATG TTAGCAGTGA TGGGAACCAA GAGAAAGCTG TTGTTGAGGT AAGTGATGTG 
CAGAAGTCTC TTAAAATTTT TGTTCACAAG GGCACTGTAA AAAGTGGAGC TCTAGAAGTT 
GGCAAGGAGG TGGAAGCAGC AGTAGATGCA GACTTGAGGC AACGAGCGAA GGTTCACCAT 
ACCGCCACTC ATTTGCTCCA ATCGGCACTT AAAAAAGTAG TAGGACAAGA AACATCACAG 
GCTGGTTCAT TAGTAGCTTT TGACCGCCTC AGATTCGATT TCAATTTTAA TCGGTCCCTG 
CATGATAATG AGCTTGAGGA AATCGAATGC CTGATCAATA GGTGGATTGG GGATGCTACA 
CGTCTTGAAA CAAAAGTCCT TCCTCTTGCT GATGCAAAAC GTGCTGGAGC CATCGCAATG 
TTTGGGGAAA AATATGATGA AAACGAGGTT CGTGTAGTAG AAGTTCCTGG TGTCTCCATG 
GAACTTTGTG GTGGCACTCA TGTTGGCAAT ACTGCAGAAA TACGAGCCTT CAAGATTATC 
TCAGAACAGG GCATTGCATC TGGAATCCGG CGTATAGAAG CGGTTGCAGG TGAAGCATTC 
ATTGAATACA TAAACTCACG GGATTCTCAA ATGACACGTC TATGCTCGAC TCTCAAGGTG 
AAAGCAGAGG ATGTTACAAA CAGAGTGGAG AATCTTCTAG AGGAACTACG TGCTGCTAGA 
AAAGAAGCCT CCGACTTGCG TTCAAAAGCA GCTGTCTATA AAGCATCTGT CATATCGAAC 
AAAGCATTTA CTGTAGGAAC TTCACAGACT ATAAGAGTGC TCGTTGAGTC GATGGATGAC 
ACCGATGCTG ACTCATTAAA GAGTGCAGCT GAGCATTTGA TAAGCACATT GGAAGATCCA 
GTCGCTGTGG TACTAGGATC ATCTCCAGAA AAAGACAAGG TTAGTTTAGT TGCTGCATTT 
AGTCCTGGAG TAGTCTCCCT AGGTGTTCAA GCAGGGAAAT TCATTGGCCC CATAGCTAAG 
CTGTGTGGCG GAGGAGGTGG TGGAAAGCCC AATTTTGCTC AGGCAGGCGG CAGAAAGCCT 
GAAAATCTCC CAAGTGCCTT AGAGAAAGCT CGGGAAGATC TCGTGGCAAC TCTATCCGAA 
AAGCTAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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EMB1030 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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