SFC cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SFC
Entrez Gene ID	831171
Full Name	ARF GTPase-activating protein
Synonyms	AGD3,ARF-GAP DOMAIN3,ASCULAR NETWORK DEFECTIVE 3,SCARFACE,T31B5.120,T31B5_120,VAN3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121333.4	NP_196834.3	ARF GTPase-activating protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that ARF-GEF GNOM and ARF-GAP vascular network defective 3 (VAN3), both of which are involved in polar auxin transport-dependent morphogenesis, localize at the plasma membranes as well as in intracellular structures.
Title: ADP-ribosylation factor machinery mediates endocytosis in plant cells.

existence of PtdIns(4)P and/or IP(3)-dependent subcellular targeting and regulation of VAN3 ACAP activity that governs plant vascular tissue continuity
Title: Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants.

Scarface (SFC) is required for normal intracellular transport of PIN1 from the plasma membrane to the endosome. [Scarface] [SFC]
Title: SCARFACE encodes an ARF-GAP that is required for normal auxin efflux and vein patterning in Arabidopsis.

VAN3 is involved in vesicle trafficking and DRP1A is responsible for the vascular formation synergistically working with the VAN3 protein. [VAN3]
Title: DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis.

expression of this gene is induced by auxin and positively regulated by VAN3 itself (VAN3)
Title: VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation.

The following SFC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb32335	NM_121333.4 Latest version!	Arabidopsis thaliana ARF GTPase-activating protein (SFC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SFC ( NM_121333.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196834.3 Arabidopsis thaliana ARF GTPase-activating protein (SFC), mRNA.

Price & Availability↑

CloneID	OAb32335
Clone ID Related Accession (Same CDS sequence)	NM_121333.4
Accession Version	NM_121333.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ARF GTPase-activating protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121333.3.

ATGCATTTCA CTAAGCTTGA TGACTCTCCC ATGTTCCGCA AACAGTTACA AAGCATGGAG 
GAGAGCGCAG AAATATTGCG GGAAAGAAGT CTCAAGTTTT ACAAAGGATG CCGGAAATAC 
ACTGAAGGAC TAGGCGAGGC ATACGATGGG GACATTGCTT TCGCAAGTGC ACTTGAAACA 
TTTGGTGGAG GCCATAATGA TCCAATAAGT GTGGCTTTTG GAGGACCGGT AATGACGAAA 
TTTACAATTG CGTTGAGGGA AATTGGGACT TATAAGGAAG TTCTTCGGTC ACAGGTGGAA 
CATATACTGA ATGACAGATT ACTTCAGTTT GCCAATATGG ACTTGCATGA AGTTAAGGAA 
GCTCGGAAGC GCTTTGACAA GGCTAGCCTT ACCTACGATC AGGCCCGTGA GAAGTTTTTA 
TCTTTGAGGA AAGGTACAAA AAGTGACGTT GCTGCTGCTT TAGAACAGGA ACTTCATACT 
TCGAGGTCTA TGTTTGAGCA AGCTCGGTTC AACCTTGTGA CTGCTCTATC AAATGTTGAA 
GCTAAGAAAA GGTTTGAATT TTTGGAAGCA GTCAGTGGAA CAATGGATGC ACATCTTCGG 
TACTTCAAAC AGGGTTACGA ATTACTGCAT CAGATGGAAC CATATATCAA TCAGGTCTTG 
ACATATGCGC AACAATCCAG AGAAAGATCA AATTATGAAC AAGCAGCACT TAATGAAAAG 
ATGCAGGAGT ACAAAAGACA GGTTGATCGA GAGAGCAGGT GGGGTTCAAA TGGTTCTAAT 
GGATCACCAA ATGGAGATGG CATACAAGCA ATCGGTAGAA GCTCTCACAA AATGATAGAC 
GCCGTAATGC AATCTGCTGC AAGAGGAAAG GTGCAAACAA TAAGGCAAGG TTATCTCTCT 
AAACGATCTT CAAACCTGAG AGGAGACTGG AAAAGAAGGT TTTTTGTTCT TGACAGCCGG 
GGAATGCTCT ATTATTACCG AAAACAGTGT AGCAAACCAT CTGGGTCTGG AAGCCAGCTT 
TCTGGACAGA GAAATAGCTC CGAGCTTGGG TCTGGACTGC TTAGTAGGTG GCTTTCTTCG 
AATAATCATG GACATGGTGG TGTCCATGAT GAGAAGTCTG TAGCTCGTCA TACAGTGAAC 
TTACTCACCT CAACAATTAA AGTCGACGCT GATCAATCAG ATCTGAGGTT TTGCTTTAGG 
ATCATATCAC CTACAAAAAA CTACACGTTG CAGGCTGAGA GTGCACTCGA TCAAATGGAT 
TGGATAGAAA AGATCACTGG GGTTATTGCA TCACTACTTA GTTCTCAGGT CCCTGAACAG 
CGTCTTCCTG GTAGTCCCAT GGGAAGTGGC CACCATCGAT CTGCTAGTGA AAGTAGCTCA 
TATGAAAGTT CTGAATATGA TCATCCTACT ACTGAAGAAT TTGTATGTGA GAGGAGCTTT 
TTGGGGTACA ATGAAAGGCC ATCAAGAAGT TTTCAGCCGC AACGGTCCAT TAGAAAGGGT 
GAGAAGCCCA TTGATGCTCT TCGAAAGGTC TGTGGGAATG ACAAATGTGC GGATTGTGGA 
GCTCCAGAAC CAGACTGGGC TTCTTTAAAT CTGGGGGTTC TTGTCTGTAT CGAATGTTCT 
GGTGTTCACC GTAATCTTGG TGTGCATATA TCTAAGGTTA GGTCACTCAC TCTCGACGTG 
AAAGTATGGG AGCCATCTGT TATAAGTTTG TTCCAAGCTC TTGGAAATAC TTTCGCAAAC 
ACAGTTTGGG AGGAATTGCT TCATTCAAGG AGCGCCATCC ATTTTGACCC CGGCTTAACA 
GTGTCTGATA AATCCAGGGT GATGGTCACT GGAAAACCCA GCTATGCTGA TATGATATCT 
ATCAAGGAGA AGTATATACA AGCTAAGTAT GCGGAGAAGT TATTTGTTCG GAGATCAAGA 
GACAGCGATT TCCCACAATC AGCTGCACAA CAAATGTGGG ACGCAGTGAG TGGCAATGAT 
AAAAAAGCTG TTTACCGGCT CATTGTCAAT GGTGATGCGG ATGTGAATTA TGTGTATGAC 
CAAACTTCCA GTTCTTCGTT AACACTTTCC AGAGTAATAT TAGTACCAGA AAGGCCAAAG 
CGTGAGGATG TTCTACTCAG ATTAAGAAAT GAGTTACTTG ATAGAACAGG TAGTTCTTCA 
AATATATCTC CTGAGGGAAG CGGAGGTTCC TCTCTGCTTC ACTGTGCTTG TGAGAAGGCA 
GACCTTGGGA TGGTAGAGCT TTTGCTGCAG TATGGTGCAA ATGTAAACGC CTCAGATTCA 
AGTGGTCAAA CGCCGCTGCA CTGTTGTCTT CTCAGAGGAA AAGTGACAAT AGCAAGATTG 
CTTCTCACTA GGGGAGCAGA CCCGGAAGCT ATGAACAGAG AAGGCAAAAC CGCTCTTGAT 
ATTGCTGCAG AGTCAAATTT TACTGATCCA GAAGTTCTTG CTCTCCTTTC AGACACAAAT 
GGATACAATC ACAGACAGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196834.3
CDS	551..3034
Translation	MHFTKLDDSPMFRKQLQSMEESAEILRERSLKFYKGCRKYTEGLGEAYDGDIAFASALETFGGGHNDPISVAFGGPVMTKFTIALREIGTYKEVLRSQVEHILNDRLLQFANMDLHEVKEARKRFDKASLTYDQAREKFLSLRKGTKSDVAAALEQELHTSRSMFEQARFNLVTALSNVEAKKRFEFLEAVSGTMDAHLRYFKQGYELLHQMEPYINQVLTYAQQSRERSNYEQAALNEKMQEYKRQVDRESRWGSNGSNGSPNGDGIQAIGRSSHKMIDAVMQSAARGKVQTIRQGYLSKRSSNLRGDWKRRFFVLDSRGMLYYYRKQCSKPSGSGSQLSGQRNSSELGSGLLSRWLSSNNHGHGGVHDEKSVARHTVNLLTSTIKVDADQSDLRFCFRIISPTKNYTLQAESALDQMDWIEKITGVIASLLSSQVPEQRLPGSPMGSGHHRSASESSSYESSEYDHPTTEEFVCERSFLGYNERPSRSFQPQRSIRKGEKPIDALRKVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQALGNTFANTVWEELLHSRSAIHFDPGLTVSDKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVRRSRDSDFPQSAAQQMWDAVSGNDKKAVYRLIVNGDADVNYVYDQTSSSSLTLSRVILVPERPKREDVLLRLRNELLDRTGSSSNISPEGSGGSSLLHCACEKADLGMVELLLQYGANVNASDSSGQTPLHCCLLRGKVTIARLLLTRGADPEAMNREGKTALDIAAESNFTDPEVLALLSDTNGYNHRQC

Target ORF information:

RefSeq Version	NM_121333.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ARF GTPase-activating protein (SFC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121333.4

ORF Insert Sequence:

ATGCATTTCA CTAAGCTTGA TGACTCTCCC ATGTTCCGCA AACAGTTACA AAGCATGGAG 
GAGAGCGCAG AAATATTGCG GGAAAGAAGT CTCAAGTTTT ACAAAGGATG CCGGAAATAC 
ACTGAAGGAC TAGGCGAGGC ATACGATGGG GACATTGCTT TCGCAAGTGC ACTTGAAACA 
TTTGGTGGAG GCCATAATGA TCCAATAAGT GTGGCTTTTG GAGGACCGGT AATGACGAAA 
TTTACAATTG CGTTGAGGGA AATTGGGACT TATAAGGAAG TTCTTCGGTC ACAGGTGGAA 
CATATACTGA ATGACAGATT ACTTCAGTTT GCCAATATGG ACTTGCATGA AGTTAAGGAA 
GCTCGGAAGC GCTTTGACAA GGCTAGCCTT ACCTACGATC AGGCCCGTGA GAAGTTTTTA 
TCTTTGAGGA AAGGTACAAA AAGTGACGTT GCTGCTGCTT TAGAACAGGA ACTTCATACT 
TCGAGGTCTA TGTTTGAGCA AGCTCGGTTC AACCTTGTGA CTGCTCTATC AAATGTTGAA 
GCTAAGAAAA GGTTTGAATT TTTGGAAGCA GTCAGTGGAA CAATGGATGC ACATCTTCGG 
TACTTCAAAC AGGGTTACGA ATTACTGCAT CAGATGGAAC CATATATCAA TCAGGTCTTG 
ACATATGCGC AACAATCCAG AGAAAGATCA AATTATGAAC AAGCAGCACT TAATGAAAAG 
ATGCAGGAGT ACAAAAGACA GGTTGATCGA GAGAGCAGGT GGGGTTCAAA TGGTTCTAAT 
GGATCACCAA ATGGAGATGG CATACAAGCA ATCGGTAGAA GCTCTCACAA AATGATAGAC 
GCCGTAATGC AATCTGCTGC AAGAGGAAAG GTGCAAACAA TAAGGCAAGG TTATCTCTCT 
AAACGATCTT CAAACCTGAG AGGAGACTGG AAAAGAAGGT TTTTTGTTCT TGACAGCCGG 
GGAATGCTCT ATTATTACCG AAAACAGTGT AGCAAACCAT CTGGGTCTGG AAGCCAGCTT 
TCTGGACAGA GAAATAGCTC CGAGCTTGGG TCTGGACTGC TTAGTAGGTG GCTTTCTTCG 
AATAATCATG GACATGGTGG TGTCCATGAT GAGAAGTCTG TAGCTCGTCA TACAGTGAAC 
TTACTCACCT CAACAATTAA AGTCGACGCT GATCAATCAG ATCTGAGGTT TTGCTTTAGG 
ATCATATCAC CTACAAAAAA CTACACGTTG CAGGCTGAGA GTGCACTCGA TCAAATGGAT 
TGGATAGAAA AGATCACTGG GGTTATTGCA TCACTACTTA GTTCTCAGGT CCCTGAACAG 
CGTCTTCCTG GTAGTCCCAT GGGAAGTGGC CACCATCGAT CTGCTAGTGA AAGTAGCTCA 
TATGAAAGTT CTGAATATGA TCATCCTACT ACTGAAGAAT TTGTATGTGA GAGGAGCTTT 
TTGGGGTACA ATGAAAGGCC ATCAAGAAGT TTTCAGCCGC AACGGTCCAT TAGAAAGGGT 
GAGAAGCCCA TTGATGCTCT TCGAAAGGTC TGTGGGAATG ACAAATGTGC GGATTGTGGA 
GCTCCAGAAC CAGACTGGGC TTCTTTAAAT CTGGGGGTTC TTGTCTGTAT CGAATGTTCT 
GGTGTTCACC GTAATCTTGG TGTGCATATA TCTAAGGTTA GGTCACTCAC TCTCGACGTG 
AAAGTATGGG AGCCATCTGT TATAAGTTTG TTCCAAGCTC TTGGAAATAC TTTCGCAAAC 
ACAGTTTGGG AGGAATTGCT TCATTCAAGG AGCGCCATCC ATTTTGACCC CGGCTTAACA 
GTGTCTGATA AATCCAGGGT GATGGTCACT GGAAAACCCA GCTATGCTGA TATGATATCT 
ATCAAGGAGA AGTATATACA AGCTAAGTAT GCGGAGAAGT TATTTGTTCG GAGATCAAGA 
GACAGCGATT TCCCACAATC AGCTGCACAA CAAATGTGGG ACGCAGTGAG TGGCAATGAT 
AAAAAAGCTG TTTACCGGCT CATTGTCAAT GGTGATGCGG ATGTGAATTA TGTGTATGAC 
CAAACTTCCA GTTCTTCGTT AACACTTTCC AGAGTAATAT TAGTACCAGA AAGGCCAAAG 
CGTGAGGATG TTCTACTCAG ATTAAGAAAT GAGTTACTTG ATAGAACAGG TAGTTCTTCA 
AATATATCTC CTGAGGGAAG CGGAGGTTCC TCTCTGCTTC ACTGTGCTTG TGAGAAGGCA 
GACCTTGGGA TGGTAGAGCT TTTGCTGCAG TATGGTGCAA ATGTAAACGC CTCAGATTCA 
AGTGGTCAAA CGCCGCTGCA CTGTTGTCTT CTCAGAGGAA AAGTGACAAT AGCAAGATTG 
CTTCTCACTA GGGGAGCAGA CCCGGAAGCT ATGAACAGAG AAGGCAAAAC CGCTCTTGAT 
ATTGCTGCAG AGTCAAATTT TACTGATCCA GAAGTTCTTG CTCTCCTTTC AGACACAAAT 
GGATACAATC ACAGACAGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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SFC cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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