AT5G12150 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT5G12150
Entrez Gene ID	831088
Full Name	Rho GTPase activation protein (RhoGAP) with PH domain-containing protein
Synonyms	MXC9.11,MXC9_11
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001125741.1	NP_001119213.1	Rho GTPase activation protein (RhoGAP) with PH domain-containing protein
NM_121253.5	NP_196776.1	Rho GTPase activation protein (RhoGAP) with PH domain-containing protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G19390	NP_197440.2
Arabidopsis thaliana (thale cress)	AT5G12150	NP_196776.1
Oryza sativa (rice)	Os07g0658300	NP_001060519.1
Oryza sativa (rice)	Os03g0356638	NP_001173426.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT5G12150 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT5G12150 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18260	NM_121253.5 Latest version!	Arabidopsis thaliana Rho GTPase activation protein (RhoGAP) with PH domain-containing protein (AT5G12150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb18261	NM_001125741.1 Latest version!	Arabidopsis thaliana Rho GTPase activation protein (RhoGAP) with PH domain-containing protein (AT5G12150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT5G12150 ( NM_121253.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196776.1 Arabidopsis thaliana Rho GTPase activation protein (RhoGAP) with PH domain-containing protein (AT5G12150), mRNA.

Price & Availability↑

CloneID	OAb18260
Clone ID Related Accession (Same CDS sequence)	NM_121253.5
Accession Version	NM_121253.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Rho GTPase activation protein (RhoGAP) with PH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121253.4.

ATGGAGGCTT CTCTAGCTGT TATACAGAGG CCTCAAGCTG GAGCTTCGAA TACGGTTTAT 
AAGAGTGGCC CTCTCTTCAT ATCATCAAAA GGACTAGGTT GGACATCCTG GAAGAAGCGT 
TGGTTTATCC TTACCCGAAC TTCTTTGGTC TTTTTCAAAA ATGATCCAAG TGCATTACCA 
CAAAAGGGAG GTGAAGTAAA TTTGACCCTT GGAGGCATTG ACTTGAACAG TTCGGGGAGT 
GTCGTGGTGA GAGAAGATAA GAAGTTGCTG ACTGTACTAT TCCCAGATGG GCGTGATGGA 
CGAGCCTTCA CCCTTAAGGC TGAGACATTG GACGACTTGT ATGAATGGAA GGCTGCCCTT 
GAGCAAGCCC TTGCACAAGC TCCCAATGCA GCACTTGTAA TTGGTCAAAA TGGAATATTC 
CGAACAGAAG CAAACAATAC TATTGAAGCA TCTTTCAATT CATGGAGGGA TCAGCGCCCG 
TTGAAGTCTT CGGTTGTTGG AAGACCAATT TTGCTAGCTT TAGAAGAAAT CGATGGAAGT 
CCATCTTTTC TTGAGAAAGC ACTTCAATTT CTTGAGACAT ATGGAACCAA AGTAGAAGGA 
ATTTTAAGAC AGTCTGCAGA TGTTGAGGAG GTAGAACGCA GAGTTCAAGA ATACGAACAA 
GGCAAGACAG AATTCAGTCC TGAAGAGGAT CCGCATGTTG TTGGAGACTG TGTTAAGCAT 
GTTCTGCGGC AATTGCCTTC TTCTCCAGTC CCAGCGTCGT GCTGCACTGC TTTGCTTGAA 
GCTTATAAAA TTGATCAGAA CGAAGCTCGG GTTAACTCAC TGCGCTCTGC TATAATTGAA 
ACATTTCCAG AACCAAACAG GCGACTACTA CTGCGGATGC TAAAGATGAT GCATACTATC 
ACCTCTCATT CCAGTGAGAA TCGCATGACT TCATCTGCTG TTGCTGCATG CATGTCCCCA 
TTGCTCTTAC GTCCTCTATT GGCTGGAGAA TGTGATCTAG AAGGTTTTGA CACTCTAGGA 
GATAACTCTG CCCAGCTTCT TGCTGCCGCC AATGCTGCCA ATAATGCTCA AGCCATTGTC 
ACAGCCCTTT TGGAAGACTA TGGGAATATG ATCAATGATG AAGGTCTTGG GAGATGCTCC 
ACTTCTACTG ATTCTCATAT TGGCGACAGT GGGCCTGAGA ACTCAAGTGA TGAAGAGGAA 
ATAGTGGTTA AACATCCTGA CTTGCATACT CTGGATATAG AAGAAGGGGA AACAGATGAT 
GATAATGATG TTCTGCTAAG CAGAAAACCA AGCGAGAGCA GTGATTATGC TGGCAGTGAT 
CTGTATGACT ACAAGGGTTT TGGTGTTGAA GATTCAGATG CTGAGTCTCC TAGAGATATC 
CATTGTTCAG TCGAGAGTAC AGACTTTTCC GCTAGAGTGA AAAAACATAT TGAAGAACCT 
ATTAAAGATA TAGAAGTTTC TAGCGTTTCA CCTACTGAAA ACTGTTATCA ATCAGGGAGA 
GAGGCTATAC CCTCAGTTAC TCCCAGTACT CCTCTCACGG CCCTTAGATA CACAACATCA 
GCTGAGAAAC CTGCAAGTAA AACTACTGGC TCATCAACAG TAAATTCGAA GCGTTCTTCG 
TCCTGGGGAA GAGGCAACGG CAAGAAAACA CCTGCTAAAG GATCATTTGA TTCCTCAGGA 
AATGACGAGC TTCTTATACA GAGGCTCGAG CACATGAAAG ATGAACTGCG ACAACGAATC 
GCTAAGGAGG CTAAAGGAAA TGCGGCTCTA CAAGCTAGCT TGGAGAGAAG GAAGCAGGCT 
TTGCACGAAC GACGGTTGGC ACTTGAACAA GATGTGGGTA GATTACAAGA GCAACTGCAA 
GCTGAGAGAG ACCTACGATC TGCTCTTGAA GTTGGTCTAA GCATTTCTTG TGGACAATTT 
AGCTCACAAG CTGCGGATTC CAAAACAAGG GCAGAGCTTG AGGAGATTGC TCTTGCTGAA 
GCTGATGTCG CAAGGTTGAA GCAGAAAGTC GCCGAATTAC ATCATCAGCT AAGTCAGCAG 
CGACAGCATC ACTTAAGTTC ACTCCCAGAT GCTCAAAGCC ATCACCAGTT TCTCCATAAT 
CACAATACTC AACTGAAGTC TTTTCAACAG GACTTTGATT CCATTCTTGC TTTCGTTAAT 
CACGAAAGAA ATCAGAGAAC GGATGAAACT AGCCTAAGAG CAGACTGGAG GAATGGCAGA 
GGAAACAACA GACAAGTACC AGGCTCACCA AGCTTGAATG CAGCATCCTT AGGCATCCCA 
ATGGAAGAGT ATTCTCCGGT AATGGACTAC GGAAGACATC ATCATCCTCC TGCAACATCT 
GCAGCTCTCA TGGAGCTAAC CACTCGTCTG GATTTCTTCA AGGAACGACG GTCACAACTT 
ATGGAACAGA TTCAGAATCT TGACCTCAAC TATGGTTCCT CTTCTTCTTC TCTGCACCGA 
TCCTCTTCTC CACCATGGAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196776.1
CDS	342..2825
Translation	MEASLAVIQRPQAGASNTVYKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPSALPQKGGEVNLTLGGIDLNSSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETLDDLYEWKAALEQALAQAPNAALVIGQNGIFRTEANNTIEASFNSWRDQRPLKSSVVGRPILLALEEIDGSPSFLEKALQFLETYGTKVEGILRQSADVEEVERRVQEYEQGKTEFSPEEDPHVVGDCVKHVLRQLPSSPVPASCCTALLEAYKIDQNEARVNSLRSAIIETFPEPNRRLLLRMLKMMHTITSHSSENRMTSSAVAACMSPLLLRPLLAGECDLEGFDTLGDNSAQLLAAANAANNAQAIVTALLEDYGNMINDEGLGRCSTSTDSHIGDSGPENSSDEEEIVVKHPDLHTLDIEEGETDDDNDVLLSRKPSESSDYAGSDLYDYKGFGVEDSDAESPRDIHCSVESTDFSARVKKHIEEPIKDIEVSSVSPTENCYQSGREAIPSVTPSTPLTALRYTTSAEKPASKTTGSSTVNSKRSSSWGRGNGKKTPAKGSFDSSGNDELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHERRLALEQDVGRLQEQLQAERDLRSALEVGLSISCGQFSSQAADSKTRAELEEIALAEADVARLKQKVAELHHQLSQQRQHHLSSLPDAQSHHQFLHNHNTQLKSFQQDFDSILAFVNHERNQRTDETSLRADWRNGRGNNRQVPGSPSLNAASLGIPMEEYSPVMDYGRHHHPPATSAALMELTTRLDFFKERRSQLMEQIQNLDLNYGSSSSSLHRSSSPPWN

Target ORF information:

RefSeq Version	NM_121253.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Rho GTPase activation protein (RhoGAP) with PH domain-containing protein (AT5G12150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121253.5

ORF Insert Sequence:

ATGGAGGCTT CTCTAGCTGT TATACAGAGG CCTCAAGCTG GAGCTTCGAA TACGGTTTAT 
AAGAGTGGCC CTCTCTTCAT ATCATCAAAA GGACTAGGTT GGACATCCTG GAAGAAGCGT 
TGGTTTATCC TTACCCGAAC TTCTTTGGTC TTTTTCAAAA ATGATCCAAG TGCATTACCA 
CAAAAGGGAG GTGAAGTAAA TTTGACCCTT GGAGGCATTG ACTTGAACAG TTCGGGGAGT 
GTCGTGGTGA GAGAAGATAA GAAGTTGCTG ACTGTACTAT TCCCAGATGG GCGTGATGGA 
CGAGCCTTCA CCCTTAAGGC TGAGACATTG GACGACTTGT ATGAATGGAA GGCTGCCCTT 
GAGCAAGCCC TTGCACAAGC TCCCAATGCA GCACTTGTAA TTGGTCAAAA TGGAATATTC 
CGAACAGAAG CAAACAATAC TATTGAAGCA TCTTTCAATT CATGGAGGGA TCAGCGCCCG 
TTGAAGTCTT CGGTTGTTGG AAGACCAATT TTGCTAGCTT TAGAAGAAAT CGATGGAAGT 
CCATCTTTTC TTGAGAAAGC ACTTCAATTT CTTGAGACAT ATGGAACCAA AGTAGAAGGA 
ATTTTAAGAC AGTCTGCAGA TGTTGAGGAG GTAGAACGCA GAGTTCAAGA ATACGAACAA 
GGCAAGACAG AATTCAGTCC TGAAGAGGAT CCGCATGTTG TTGGAGACTG TGTTAAGCAT 
GTTCTGCGGC AATTGCCTTC TTCTCCAGTC CCAGCGTCGT GCTGCACTGC TTTGCTTGAA 
GCTTATAAAA TTGATCAGAA CGAAGCTCGG GTTAACTCAC TGCGCTCTGC TATAATTGAA 
ACATTTCCAG AACCAAACAG GCGACTACTA CTGCGGATGC TAAAGATGAT GCATACTATC 
ACCTCTCATT CCAGTGAGAA TCGCATGACT TCATCTGCTG TTGCTGCATG CATGTCCCCA 
TTGCTCTTAC GTCCTCTATT GGCTGGAGAA TGTGATCTAG AAGGTTTTGA CACTCTAGGA 
GATAACTCTG CCCAGCTTCT TGCTGCCGCC AATGCTGCCA ATAATGCTCA AGCCATTGTC 
ACAGCCCTTT TGGAAGACTA TGGGAATATG ATCAATGATG AAGGTCTTGG GAGATGCTCC 
ACTTCTACTG ATTCTCATAT TGGCGACAGT GGGCCTGAGA ACTCAAGTGA TGAAGAGGAA 
ATAGTGGTTA AACATCCTGA CTTGCATACT CTGGATATAG AAGAAGGGGA AACAGATGAT 
GATAATGATG TTCTGCTAAG CAGAAAACCA AGCGAGAGCA GTGATTATGC TGGCAGTGAT 
CTGTATGACT ACAAGGGTTT TGGTGTTGAA GATTCAGATG CTGAGTCTCC TAGAGATATC 
CATTGTTCAG TCGAGAGTAC AGACTTTTCC GCTAGAGTGA AAAAACATAT TGAAGAACCT 
ATTAAAGATA TAGAAGTTTC TAGCGTTTCA CCTACTGAAA ACTGTTATCA ATCAGGGAGA 
GAGGCTATAC CCTCAGTTAC TCCCAGTACT CCTCTCACGG CCCTTAGATA CACAACATCA 
GCTGAGAAAC CTGCAAGTAA AACTACTGGC TCATCAACAG TAAATTCGAA GCGTTCTTCG 
TCCTGGGGAA GAGGCAACGG CAAGAAAACA CCTGCTAAAG GATCATTTGA TTCCTCAGGA 
AATGACGAGC TTCTTATACA GAGGCTCGAG CACATGAAAG ATGAACTGCG ACAACGAATC 
GCTAAGGAGG CTAAAGGAAA TGCGGCTCTA CAAGCTAGCT TGGAGAGAAG GAAGCAGGCT 
TTGCACGAAC GACGGTTGGC ACTTGAACAA GATGTGGGTA GATTACAAGA GCAACTGCAA 
GCTGAGAGAG ACCTACGATC TGCTCTTGAA GTTGGTCTAA GCATTTCTTG TGGACAATTT 
AGCTCACAAG CTGCGGATTC CAAAACAAGG GCAGAGCTTG AGGAGATTGC TCTTGCTGAA 
GCTGATGTCG CAAGGTTGAA GCAGAAAGTC GCCGAATTAC ATCATCAGCT AAGTCAGCAG 
CGACAGCATC ACTTAAGTTC ACTCCCAGAT GCTCAAAGCC ATCACCAGTT TCTCCATAAT 
CACAATACTC AACTGAAGTC TTTTCAACAG GACTTTGATT CCATTCTTGC TTTCGTTAAT 
CACGAAAGAA ATCAGAGAAC GGATGAAACT AGCCTAAGAG CAGACTGGAG GAATGGCAGA 
GGAAACAACA GACAAGTACC AGGCTCACCA AGCTTGAATG CAGCATCCTT AGGCATCCCA 
ATGGAAGAGT ATTCTCCGGT AATGGACTAC GGAAGACATC ATCATCCTCC TGCAACATCT 
GCAGCTCTCA TGGAGCTAAC CACTCGTCTG GATTTCTTCA AGGAACGACG GTCACAACTT 
ATGGAACAGA TTCAGAATCT TGACCTCAAC TATGGTTCCT CTTCTTCTTC TCTGCACCGA 
TCCTCTTCTC CACCATGGAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT5G12150 ( NM_001125741.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119213.1 Arabidopsis thaliana Rho GTPase activation protein (RhoGAP) with PH domain-containing protein (AT5G12150), mRNA.

Price & Availability↑

CloneID	OAb18261
Clone ID Related Accession (Same CDS sequence)	NM_001125741.1
Accession Version	NM_001125741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Rho GTPase activation protein (RhoGAP) with PH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGAGGCTT CTCTAGCTGT TATACAGAGG CCTCAAGCTG GAGCTTCGAA TACGGTTTAT 
AAGAGTGGCC CTCTCTTCAT ATCATCAAAA GGACTAGGTT GGACATCCTG GAAGAAGCGT 
TGGTTTATCC TTACCCGAAC TTCTTTGGTC TTTTTCAAAA ATGATCCAAG TGCATTACCA 
CAAAAGGGAG GTGAAGTAAA TTTGACCCTT GGAGGCATTG ACTTGAACAG TTCGGGGAGT 
GTCGTGGTGA GAGAAGATAA GAAGTTGCTG ACTGTACTAT TCCCAGATGG GCGTGATGGA 
CGAGCCTTCA CCCTTAAGGC TGAGACATTG GACGACTTGT ATGAATGGAA GGCTGCCCTT 
GAGCAAGCCC TTGCACAAGC TCCCAATGCA GCACTTGTAA TTGGTCAAAA TGGAATATTC 
CGAACAGAAG CAAACAATAC TATTGAAGCA TCTTTCAATT CATGGAGGGA TCAGCGCCCG 
TTGAAGTCTT CGGTTGTTGG AAGACCAATT TTGCTAGCTT TAGAAGAAAT CGATGGAAGT 
CCATCTTTTC TTGAGAAAGC ACTTCAATTT CTTGAGACAT ATGGAACCAA AGTAGAAGGA 
ATTTTAAGAC AGTCTGCAGA TGTTGAGGAG GTAGAACGCA GAGTTCAAGA ATACGAACAA 
GGCAAGACAG AATTCAGTCC TGAAGAGGAT CCGCATGTTG TTGGAGACTG TGTTAAGCAT 
GTTCTGCGGC AATTGCCTTC TTCTCCAGTC CCAGCGTCGT GCTGCACTGC TTTGCTTGAA 
GCTTATAAAA TTGATCAGAA CGAAGCTCGG GTTAACTCAC TGCGCTCTGC TATAATTGAA 
ACATTTCCAG AACCAAACAG GCGACTACTA CTGCGGATGC TAAAGATGAT GCATACTATC 
ACCTCTCATT CCAGTGAGAA TCGCATGACT TCATCTGCTG TTGCTGCATG CATGTCCCCA 
TTGCTCTTAC GTCCTCTATT GGCTGGAGAA TGTGATCTAG AAGGTTTTGA CACTCTAGGA 
GATAACTCTG CCCAGCTTCT TGCTGCCGCC AATGCTGCCA ATAATGCTCA AGCCATTGTC 
ACAGCCCTTT TGGAAGACTA TGGGAATATG ATCAATGATG AAGGTCTTGG GAGATGCTCC 
ACTTCTACTG ATTCTCATAT TGGCGACAGT GGGCCTGAGA ACTCAAGTGA TGAAGAGGAA 
ATAGTGGTTA AACATCCTGA CTTGCATACT CTGGATATAG AAGAAGGGGA AACAGATGAT 
GATAATGATG TTCTGCTAAG CAGAAAACCA AGCGAGAGCA GTGATTATGC TGGCAGTGAT 
CTGTATGACT ACAAGGGTTT TGGTGTTGAA GATTCAGATG CTGAGTCTCC TAGAGATATC 
CATTGTTCAG TCGAGAGTAC AGACTTTTCC GCTAGAGTGA AAAAACATAT TGAAGAACCT 
ATTAAAGATA TAGAAGTTTC TAGCGTTTCA CCTACTGAAA ACTGTTATCA ATCAGGGAGA 
GAGGCTATAC CCTCAGTTAC TCCCAGTACT CCTCTCACGG CCCTTAGATA CACAACATCA 
GCTGAGAAAC CTGCAAGTAA AACTACTGGC TCATCAACAG TAAATTCGAA GCGTTCTTCG 
TCCTGGGGAA GAGGCAACGG CAAGAAAACA CCTGCTAAAG GATCATTTGA TTCCTCAGGA 
AATGACGAGC TTCTTATACA GAGGCTCGAG CACATGAAAG ATGAACTGCG ACAACGAATC 
GCTAAGGAGG CTAAAGGAAA TGCGGCTCTA CAAGCTAGCT TGGAGAGAAG GAAGCAGGCT 
TTGCACGAAC GACGGTTGGC ACTTGAACAA GATGTGGGTA GATTACAAGA GCAACTGCAA 
GCTGAGAGAG ACCTACGATC TGCTCTTGAA GTTGGTCTAA GCATTTCTTG TGGACAATTT 
AGCTCACAAG CTGCGGATTC CAAAACAAGG GCAGAGCTTG AGGAGATTGC TCTTGCTGAA 
GCTGATGTCG CAAGGTTGAA GCAGAAAGTC GCCGAATTAC ATCATCAGCT AAGTCAGCAG 
CGACAGCATC ACTTAAGTTC ACTCCCAGAT GCTCAAAGCC ATCACCAGTT TCTCCATAAT 
CACAATACTC AACTGAAGTC TTTTCAACAG GACTTTGATT CCATTCTTGC TTTCGTTAAT 
CACGAAAGAA ATCAGAGAAC GGATGAAACT AGCCTAAGAG CAGACTGGAG GAATGGCAGA 
GGAAACAACA GACAAGTACC AGGCTCACCA AGCTTGAATG CAGCATCCTT AGGCATCCCA 
ATGGAAGAGT ATTCTCCGGT TAGTGATAGT CTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119213.1
CDS	206..2524
Translation	MEASLAVIQRPQAGASNTVYKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPSALPQKGGEVNLTLGGIDLNSSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETLDDLYEWKAALEQALAQAPNAALVIGQNGIFRTEANNTIEASFNSWRDQRPLKSSVVGRPILLALEEIDGSPSFLEKALQFLETYGTKVEGILRQSADVEEVERRVQEYEQGKTEFSPEEDPHVVGDCVKHVLRQLPSSPVPASCCTALLEAYKIDQNEARVNSLRSAIIETFPEPNRRLLLRMLKMMHTITSHSSENRMTSSAVAACMSPLLLRPLLAGECDLEGFDTLGDNSAQLLAAANAANNAQAIVTALLEDYGNMINDEGLGRCSTSTDSHIGDSGPENSSDEEEIVVKHPDLHTLDIEEGETDDDNDVLLSRKPSESSDYAGSDLYDYKGFGVEDSDAESPRDIHCSVESTDFSARVKKHIEEPIKDIEVSSVSPTENCYQSGREAIPSVTPSTPLTALRYTTSAEKPASKTTGSSTVNSKRSSSWGRGNGKKTPAKGSFDSSGNDELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHERRLALEQDVGRLQEQLQAERDLRSALEVGLSISCGQFSSQAADSKTRAELEEIALAEADVARLKQKVAELHHQLSQQRQHHLSSLPDAQSHHQFLHNHNTQLKSFQQDFDSILAFVNHERNQRTDETSLRADWRNGRGNNRQVPGSPSLNAASLGIPMEEYSPVSDSLV

Target ORF information:

RefSeq Version	NM_001125741.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Rho GTPase activation protein (RhoGAP) with PH domain-containing protein (AT5G12150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125741.1

ORF Insert Sequence:

ATGGAGGCTT CTCTAGCTGT TATACAGAGG CCTCAAGCTG GAGCTTCGAA TACGGTTTAT 
AAGAGTGGCC CTCTCTTCAT ATCATCAAAA GGACTAGGTT GGACATCCTG GAAGAAGCGT 
TGGTTTATCC TTACCCGAAC TTCTTTGGTC TTTTTCAAAA ATGATCCAAG TGCATTACCA 
CAAAAGGGAG GTGAAGTAAA TTTGACCCTT GGAGGCATTG ACTTGAACAG TTCGGGGAGT 
GTCGTGGTGA GAGAAGATAA GAAGTTGCTG ACTGTACTAT TCCCAGATGG GCGTGATGGA 
CGAGCCTTCA CCCTTAAGGC TGAGACATTG GACGACTTGT ATGAATGGAA GGCTGCCCTT 
GAGCAAGCCC TTGCACAAGC TCCCAATGCA GCACTTGTAA TTGGTCAAAA TGGAATATTC 
CGAACAGAAG CAAACAATAC TATTGAAGCA TCTTTCAATT CATGGAGGGA TCAGCGCCCG 
TTGAAGTCTT CGGTTGTTGG AAGACCAATT TTGCTAGCTT TAGAAGAAAT CGATGGAAGT 
CCATCTTTTC TTGAGAAAGC ACTTCAATTT CTTGAGACAT ATGGAACCAA AGTAGAAGGA 
ATTTTAAGAC AGTCTGCAGA TGTTGAGGAG GTAGAACGCA GAGTTCAAGA ATACGAACAA 
GGCAAGACAG AATTCAGTCC TGAAGAGGAT CCGCATGTTG TTGGAGACTG TGTTAAGCAT 
GTTCTGCGGC AATTGCCTTC TTCTCCAGTC CCAGCGTCGT GCTGCACTGC TTTGCTTGAA 
GCTTATAAAA TTGATCAGAA CGAAGCTCGG GTTAACTCAC TGCGCTCTGC TATAATTGAA 
ACATTTCCAG AACCAAACAG GCGACTACTA CTGCGGATGC TAAAGATGAT GCATACTATC 
ACCTCTCATT CCAGTGAGAA TCGCATGACT TCATCTGCTG TTGCTGCATG CATGTCCCCA 
TTGCTCTTAC GTCCTCTATT GGCTGGAGAA TGTGATCTAG AAGGTTTTGA CACTCTAGGA 
GATAACTCTG CCCAGCTTCT TGCTGCCGCC AATGCTGCCA ATAATGCTCA AGCCATTGTC 
ACAGCCCTTT TGGAAGACTA TGGGAATATG ATCAATGATG AAGGTCTTGG GAGATGCTCC 
ACTTCTACTG ATTCTCATAT TGGCGACAGT GGGCCTGAGA ACTCAAGTGA TGAAGAGGAA 
ATAGTGGTTA AACATCCTGA CTTGCATACT CTGGATATAG AAGAAGGGGA AACAGATGAT 
GATAATGATG TTCTGCTAAG CAGAAAACCA AGCGAGAGCA GTGATTATGC TGGCAGTGAT 
CTGTATGACT ACAAGGGTTT TGGTGTTGAA GATTCAGATG CTGAGTCTCC TAGAGATATC 
CATTGTTCAG TCGAGAGTAC AGACTTTTCC GCTAGAGTGA AAAAACATAT TGAAGAACCT 
ATTAAAGATA TAGAAGTTTC TAGCGTTTCA CCTACTGAAA ACTGTTATCA ATCAGGGAGA 
GAGGCTATAC CCTCAGTTAC TCCCAGTACT CCTCTCACGG CCCTTAGATA CACAACATCA 
GCTGAGAAAC CTGCAAGTAA AACTACTGGC TCATCAACAG TAAATTCGAA GCGTTCTTCG 
TCCTGGGGAA GAGGCAACGG CAAGAAAACA CCTGCTAAAG GATCATTTGA TTCCTCAGGA 
AATGACGAGC TTCTTATACA GAGGCTCGAG CACATGAAAG ATGAACTGCG ACAACGAATC 
GCTAAGGAGG CTAAAGGAAA TGCGGCTCTA CAAGCTAGCT TGGAGAGAAG GAAGCAGGCT 
TTGCACGAAC GACGGTTGGC ACTTGAACAA GATGTGGGTA GATTACAAGA GCAACTGCAA 
GCTGAGAGAG ACCTACGATC TGCTCTTGAA GTTGGTCTAA GCATTTCTTG TGGACAATTT 
AGCTCACAAG CTGCGGATTC CAAAACAAGG GCAGAGCTTG AGGAGATTGC TCTTGCTGAA 
GCTGATGTCG CAAGGTTGAA GCAGAAAGTC GCCGAATTAC ATCATCAGCT AAGTCAGCAG 
CGACAGCATC ACTTAAGTTC ACTCCCAGAT GCTCAAAGCC ATCACCAGTT TCTCCATAAT 
CACAATACTC AACTGAAGTC TTTTCAACAG GACTTTGATT CCATTCTTGC TTTCGTTAAT 
CACGAAAGAA ATCAGAGAAC GGATGAAACT AGCCTAAGAG CAGACTGGAG GAATGGCAGA 
GGAAACAACA GACAAGTACC AGGCTCACCA AGCTTGAATG CAGCATCCTT AGGCATCCCA 
ATGGAAGAGT ATTCTCCGGT TAGTGATAGT CTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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AT5G12150 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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