NEDD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEDD1
Entrez Gene ID	830483
Full Name	Transducin/WD40 repeat-like superfamily protein
Synonyms	DEVELOPMENTALLY DOWN-REGULATED GENE 1,K18J17.16,K18J17_16,NEURAL PRECURSOR CELL EXPRESSED
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_120679.3	NP_196216.2	Transducin/WD40 repeat-like superfamily protein
NM_001125704.1	NP_001119176.1	Transducin/WD40 repeat-like superfamily protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os09g0267500	NP_001062722.1
Arabidopsis thaliana (thale cress)	NEDD1	NP_196216.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of NEDD1 resulted in reduction of gamma-TuRC recruitment to cortical microtubules and total nucleation frequency, showing that GCP-WD/NEDD1 controls gamma-TuRC positioning and function
Title: GCP-WD mediates ?-TuRC recruitment and the geometry of microtubule nucleation in interphase arrays of Arabidopsis.

NEDD1 homolog plays a critical role in MT organization during mitosis.
Title: The WD40 repeat protein NEDD1 functions in microtubule organization during cell division in Arabidopsis thaliana.

The following NEDD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb17736	NM_001125704.1 Latest version!	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (NEDD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb17737	NM_120679.3 Latest version!	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (NEDD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEDD1 ( NM_001125704.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119176.1 Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (NEDD1), mRNA.

Price & Availability↑

CloneID	OAb17736
Clone ID Related Accession (Same CDS sequence)	NM_001125704.1
Accession Version	NM_001125704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transducin/WD40 repeat-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGATGTCGA ACTTGGTAGA GCCATCGTGG CGCCTTCTGG CAGCGAGCGG CGGAGATACG 
GTTAAGCTTT TCGACGTTTC TGCCGATTCC GGCGATCCCT GCGTTTTGAG CTATACTCCG 
TCACCTGGTT GTGCTGTCAA CTCCGTCAAG TGGAATCATA CGAATTTAGT GGTGGCCAGT 
ACGGGAGAAG ATAAGAAGAT ATCCCTGTGG CGGAAAAATG GGCAGAGTTT AGGAACAGTT 
CCAGTTACTG GGAAGGACGG TGGTGATAGT GCTGAGGAAT GTTTATCGGC TATTAGCTTC 
AGCAAAAAAG GCTCCAGGTA CATTTGTTCT GGAGGAACTG GTCAAATTGT TAAGATATGG 
GATCTGCAAA GAAAGCTTTG TATTAAGAAG TTGAAAGGTC ATACAAGTAC AATAACGGGT 
GTGATGTACA ATTGCAAAGA TGAGCATTTG GCTTCTGTTA GTGTTGGTGG GGATCTAATA 
GTCCATAACC TTGCATCTGG AGCAAGGGCT ACCGAACTCA AAGATCCAAA TGGGCAGGTA 
TTAAGGTTGC TTGATTATTC AAGGTCTAGT CGACACCTCC TAGTTACTGC AGGTGATGAT 
GGCACTGTAC ATCTGTGGGA CACAACTGGT CGTAGCCCGA AGATGTCCTG GTTGAAGCAG 
CATTCGGCAC CAACTGCTGG TGTTTGCTTC TCTCCATCAA ACGAAAAGAT AATTGCTAGT 
GTCGGGATGG ACAAAAAGCT TTACACTTAT GATTCTGGAT CCAGAAGATC TTCGTCCTGC 
ATTGCCTATG AGGCACCTTT CTCCTCTTTG GCGTTTGGGG ATAATGGCTA TATTCTGGTA 
GCTGGAACCA GTAACGGTAG AGTAGTGTTT TACGACATCC GTGGAAAACC GCAGCCTGTT 
ACTGTCCTGC ACGCTTTCAG TAATTCAGAG GATGTAACAA GTTTATCATG GCAAACATCA 
AAACCAGTTA TTGTGAATGA GAAAAATTAC ACTTCTGAGA TGGCTCTTCT TGGCAGTACT 
GTGGAAGATT CAGTTGTCAT TCCAGATCCA CTTCCCTCAA CGACACCCTC AGCTTCACAG 
TCCGCTATGG CACCAGGTTC CCGTGGAGTA TCCGCGAGTA CTGTAAATGC ATCATCAGTT 
GAACAAACAC CAAATAGAAA TCATCTATGG CCTAGTGGGC CACTGGGCAG ACTGCATGCA 
CTTCGTGCAA ATGACAGTTA TAATGATGAT ATGGGTGTTT TTTCACCTAT TATTGACGTA 
TCATCTGTTG AGAAGTGGGC TGATAGTGAA GGATATAATA ATAAGGACCA TTTAGTTGTT 
GACAACAAGC CGTCCTCTTT GCTATTTCCG TCATCCAGCA AGGGATATTC GTTTGGAGAC 
AATGGAAGCA AGGAACATCC AATATTCGAT TGGAAACCAA GCTCTACTTC GAAACAGGAT 
GACCCGAGGG CTGCTTTTTC GTCTTTTGGA TCTATTACGC CAACAGCATC ATCCAAAAGT 
GAAGACTCTG CCTTGACACC TCCAGAAGCA TGGGGTGGTG ATAAATTTTC TGAGAAGTTT 
AACCAATTGG CCAATGAGAA ATTTTCTGAT AAATTTAGCC ACCTGCATGC ACCTTCACGT 
CTCGCTGTTT CAAGCACAGG TGCTAGTACA TCTGGATCAA TGTTCTCTAG CTCTCGAGAT 
TTCCCCTTGT CGCATGGTCA GACAAACTTC GCAAACGCTA GCTTAGAATT CCCACGGATC 
AGAGACTTCT CTTCAACCTT TGAGACATCC TCAACACAGA CAGACAACAA CTTGCCTTCA 
AGCCCATTGT TTACAAAAGG CATCACAGCT CCTGGTAACA TTGATTCGTT GAGGCTATCA 
CCAAATTTTA CTCGTAGGTT CTCAACATAT GCTGAGAGAA TCAGCACCAC TTCTTCCTTT 
AGTGACGGGG CCTCTCTCAG TCTGGGTGGC TCACCCAAGA TAAAGAAAAC AGGCTCAGAA 
ACGAGAGAGG AAGTTTTAAA TCATCTGTTG GCAAGACCAG AGACAGTAGT TGCAACAGAA 
GCAGGAGCTA TGCCGCTTAT GAATCAGGGA GGGTTGAAGC AATCTCAAAC GGATCAACAG 
CAAGTGATGG GTAGTAGCAA TTTCACGCTT CAGCTATTTC AAAGAACGCT CGAAGGAACT 
CTGGATTCTT TCCAAAACTC AATTCATGAC GACGTAAGGA ACCTTCACAT TGAGATCCTC 
AGACAGTTCC ACATGCACGA GATGGAGATG TCGAAGGTGT TAAGCTCGAT TCTGGAGAAC 
CAAGCGGAGC AAATGAAGGA GTTAAAGTTG TTGCGAAAAG AGAATCAAGA GCTCAGACAA 
AGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119176.1
CDS	171..2519
Translation	MMSNLVEPSWRLLAASGGDTVKLFDVSADSGDPCVLSYTPSPGCAVNSVKWNHTNLVVASTGEDKKISLWRKNGQSLGTVPVTGKDGGDSAEECLSAISFSKKGSRYICSGGTGQIVKIWDLQRKLCIKKLKGHTSTITGVMYNCKDEHLASVSVGGDLIVHNLASGARATELKDPNGQVLRLLDYSRSSRHLLVTAGDDGTVHLWDTTGRSPKMSWLKQHSAPTAGVCFSPSNEKIIASVGMDKKLYTYDSGSRRSSSCIAYEAPFSSLAFGDNGYILVAGTSNGRVVFYDIRGKPQPVTVLHAFSNSEDVTSLSWQTSKPVIVNEKNYTSEMALLGSTVEDSVVIPDPLPSTTPSASQSAMAPGSRGVSASTVNASSVEQTPNRNHLWPSGPLGRLHALRANDSYNDDMGVFSPIIDVSSVEKWADSEGYNNKDHLVVDNKPSSLLFPSSSKGYSFGDNGSKEHPIFDWKPSSTSKQDDPRAAFSSFGSITPTASSKSEDSALTPPEAWGGDKFSEKFNQLANEKFSDKFSHLHAPSRLAVSSTGASTSGSMFSSSRDFPLSHGQTNFANASLEFPRIRDFSSTFETSSTQTDNNLPSSPLFTKGITAPGNIDSLRLSPNFTRRFSTYAERISTTSSFSDGASLSLGGSPKIKKTGSETREEVLNHLLARPETVVATEAGAMPLMNQGGLKQSQTDQQQVMGSSNFTLQLFQRTLEGTLDSFQNSIHDDVRNLHIEILRQFHMHEMEMSKVLSSILENQAEQMKELKLLRKENQELRQRL

Target ORF information:

RefSeq Version	NM_001125704.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (NEDD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125704.1

ORF Insert Sequence:

ATGATGTCGA ACTTGGTAGA GCCATCGTGG CGCCTTCTGG CAGCGAGCGG CGGAGATACG 
GTTAAGCTTT TCGACGTTTC TGCCGATTCC GGCGATCCCT GCGTTTTGAG CTATACTCCG 
TCACCTGGTT GTGCTGTCAA CTCCGTCAAG TGGAATCATA CGAATTTAGT GGTGGCCAGT 
ACGGGAGAAG ATAAGAAGAT ATCCCTGTGG CGGAAAAATG GGCAGAGTTT AGGAACAGTT 
CCAGTTACTG GGAAGGACGG TGGTGATAGT GCTGAGGAAT GTTTATCGGC TATTAGCTTC 
AGCAAAAAAG GCTCCAGGTA CATTTGTTCT GGAGGAACTG GTCAAATTGT TAAGATATGG 
GATCTGCAAA GAAAGCTTTG TATTAAGAAG TTGAAAGGTC ATACAAGTAC AATAACGGGT 
GTGATGTACA ATTGCAAAGA TGAGCATTTG GCTTCTGTTA GTGTTGGTGG GGATCTAATA 
GTCCATAACC TTGCATCTGG AGCAAGGGCT ACCGAACTCA AAGATCCAAA TGGGCAGGTA 
TTAAGGTTGC TTGATTATTC AAGGTCTAGT CGACACCTCC TAGTTACTGC AGGTGATGAT 
GGCACTGTAC ATCTGTGGGA CACAACTGGT CGTAGCCCGA AGATGTCCTG GTTGAAGCAG 
CATTCGGCAC CAACTGCTGG TGTTTGCTTC TCTCCATCAA ACGAAAAGAT AATTGCTAGT 
GTCGGGATGG ACAAAAAGCT TTACACTTAT GATTCTGGAT CCAGAAGATC TTCGTCCTGC 
ATTGCCTATG AGGCACCTTT CTCCTCTTTG GCGTTTGGGG ATAATGGCTA TATTCTGGTA 
GCTGGAACCA GTAACGGTAG AGTAGTGTTT TACGACATCC GTGGAAAACC GCAGCCTGTT 
ACTGTCCTGC ACGCTTTCAG TAATTCAGAG GATGTAACAA GTTTATCATG GCAAACATCA 
AAACCAGTTA TTGTGAATGA GAAAAATTAC ACTTCTGAGA TGGCTCTTCT TGGCAGTACT 
GTGGAAGATT CAGTTGTCAT TCCAGATCCA CTTCCCTCAA CGACACCCTC AGCTTCACAG 
TCCGCTATGG CACCAGGTTC CCGTGGAGTA TCCGCGAGTA CTGTAAATGC ATCATCAGTT 
GAACAAACAC CAAATAGAAA TCATCTATGG CCTAGTGGGC CACTGGGCAG ACTGCATGCA 
CTTCGTGCAA ATGACAGTTA TAATGATGAT ATGGGTGTTT TTTCACCTAT TATTGACGTA 
TCATCTGTTG AGAAGTGGGC TGATAGTGAA GGATATAATA ATAAGGACCA TTTAGTTGTT 
GACAACAAGC CGTCCTCTTT GCTATTTCCG TCATCCAGCA AGGGATATTC GTTTGGAGAC 
AATGGAAGCA AGGAACATCC AATATTCGAT TGGAAACCAA GCTCTACTTC GAAACAGGAT 
GACCCGAGGG CTGCTTTTTC GTCTTTTGGA TCTATTACGC CAACAGCATC ATCCAAAAGT 
GAAGACTCTG CCTTGACACC TCCAGAAGCA TGGGGTGGTG ATAAATTTTC TGAGAAGTTT 
AACCAATTGG CCAATGAGAA ATTTTCTGAT AAATTTAGCC ACCTGCATGC ACCTTCACGT 
CTCGCTGTTT CAAGCACAGG TGCTAGTACA TCTGGATCAA TGTTCTCTAG CTCTCGAGAT 
TTCCCCTTGT CGCATGGTCA GACAAACTTC GCAAACGCTA GCTTAGAATT CCCACGGATC 
AGAGACTTCT CTTCAACCTT TGAGACATCC TCAACACAGA CAGACAACAA CTTGCCTTCA 
AGCCCATTGT TTACAAAAGG CATCACAGCT CCTGGTAACA TTGATTCGTT GAGGCTATCA 
CCAAATTTTA CTCGTAGGTT CTCAACATAT GCTGAGAGAA TCAGCACCAC TTCTTCCTTT 
AGTGACGGGG CCTCTCTCAG TCTGGGTGGC TCACCCAAGA TAAAGAAAAC AGGCTCAGAA 
ACGAGAGAGG AAGTTTTAAA TCATCTGTTG GCAAGACCAG AGACAGTAGT TGCAACAGAA 
GCAGGAGCTA TGCCGCTTAT GAATCAGGGA GGGTTGAAGC AATCTCAAAC GGATCAACAG 
CAAGTGATGG GTAGTAGCAA TTTCACGCTT CAGCTATTTC AAAGAACGCT CGAAGGAACT 
CTGGATTCTT TCCAAAACTC AATTCATGAC GACGTAAGGA ACCTTCACAT TGAGATCCTC 
AGACAGTTCC ACATGCACGA GATGGAGATG TCGAAGGTGT TAAGCTCGAT TCTGGAGAAC 
CAAGCGGAGC AAATGAAGGA GTTAAAGTTG TTGCGAAAAG AGAATCAAGA GCTCAGACAA 
AGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD1 ( NM_120679.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196216.2 Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (NEDD1), mRNA.

Price & Availability↑

CloneID	OAb17737
Clone ID Related Accession (Same CDS sequence)	NM_120679.3
Accession Version	NM_120679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transducin/WD40 repeat-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 18, 2007 this sequence version replaced NM_120679.2.

ATGATGTCGA ACTTGGTAGA GCCATCGTGG CGCCTTCTGG CAGCGAGCGG CGGAGATACG 
GTTAAGCTTT TCGACGTTTC TGCCGATTCC GGCGATCCCT GCGTTTTGAG CTATACTCCG 
TCACCTGGTT GTGCTGTCAA CTCCGTCAAG TGGAATCATA CGAATTTAGT GGTGGCCAGT 
ACGGGAGAAG ATAAGAAGAT ATCCCTGTGG CGGAAAAATG GGCAGAGTTT AGGAACAGTT 
CCAGTTACTG GGAAGGACGG TGGTGATAGT GCTGAGGAAT GTTTATCGGC TATTAGCTTC 
AGCAAAAAAG GCTCCAGGTA CATTTGTTCT GGAGGAACTG GTCAAATTGT TAAGATATGG 
GATCTGCAAA GAAAGCTTTG TATTAAGAAG TTGAAAGGTC ATACAAGTAC AATAACGGGT 
GTGATGTACA ATTGCAAAGA TGAGCATTTG GCTTCTGTTA GTGTTGGTGG GGATCTAATA 
GTCCATAACC TTGCATCTGG AGCAAGGGCT ACCGAACTCA AAGATCCAAA TGGGCAGGTA 
TTAAGGTTGC TTGATTATTC AAGGTCTAGT CGACACCTCC TAGTTACTGC AGGTGATGAT 
GGCACTGTAC ATCTGTGGGA CACAACTGGT CGTAGCCCGA AGATGTCCTG GTTGAAGCAG 
CATTCGGCAC CAACTGCTGG TGTTTGCTTC TCTCCATCAA ACGAAAAGAT AATTGCTAGT 
GTCGGGATGG ACAAAAAGCT TTACACTTAT GATTCTGGAT CCAGAAGATC TTCGTCCTGC 
ATTGCCTATG AGGCACCTTT CTCCTCTTTG GCGTTTGGGG ATAATGGCTA TATTCTGGTA 
GCTGGAACCA GTAACGGTAG AGTAGTGTTT TACGACATCC GTGGAAAACC GCAGCCTGTT 
ACTGTCCTGC ACGCTTTCAG TAATTCAGAG GATGTAACAA GTTTATCATG GCAAACATCA 
AAACCAGTTA TTGTGAATGA GAAAAATTAC ACTTCTGAGA TGGCTCTTCT TGGCAGTACT 
GTGGAAGATT CAGTTGTCAT TCCAGATCCA CTTCCCTCAA CGACACCCTC AGCTTCACAG 
TCCGCTATGG CACCAGGTTC CCGTGGAGTA TCCGCGAGTA CTGTAAATGC ATCATCAGTT 
GAACAAACAC CAAATAGAAA TCATCTATGG CCTAGTGGGC CACTGGGCAG ACTGCATGCA 
CTTCGTGCAA ATGACAGTTA TAATGATGAT ATGGGTGTTT TTTCACCTAT TATTGACGTA 
TCATCTGTTG AGAAGTGGGC TGATAGTGAA GGATATAATA ATAAGGACCA TTTAGTTGTT 
GACAACAAGC CGTCCTCTTT GCTATTTCCG TCATCCAGCA AGGGATATTC GTTTGGAGAC 
AATGGAAGCA AGGAACATCC AATATTCGAT TGGAAACCAA GCTCTACTTC GAAACAGGAT 
GACCCGAGGG CTGCTTTTTC GTCTTTTGGA TCTATTACGC CAACAGCATC ATCCAAAAGT 
GAAGACTCTG CCTTGACACC TCCAGAAGCA TGGGGTGGTG ATAAATTTTC TGAGAAGTTT 
AACCAATTGG CCAATGAGAA ATTTTCTGAT AAATTTAGCC ACCTGCATGC ACCTTCACGT 
CTCGCTGTTT CAAGCACAGG TGCTAGTACA TCTGGATCAA TGTTCTCTAG CTCTCGAGAT 
TTCCCCTTGT CGCATGGTCA GACAAACTTC GCAAACGCTA GCTTAGAATT CCCACGGATC 
AGAGACTTCT CTTCAACCTT TGAGACATCC TCAACACAGA CAGACAACAA CTTGCCTTCA 
AGCCCATTGT TTACAAAAGG CATCACAGCT CCTGGTAACA TTGATTCGTT GAGGCTATCA 
CCAAATTTTA CTCGTAGGTT CTCAACATAT GCTGAGAGAA TCAGCACCAC TTCTTCCTTT 
AGTGACGGGG CCTCTCTCAG TCTGGGTGGC TCACCCAAGA TAAAGAAAAC AGGCTCAGAA 
ACGAGAGAGG AAGTTTTAAA TCATCTGTTG GCAAGACCAG AGACAGTAGT TGCAACAGAA 
GCAGGAGCTA TGCCGCTTAT GAATGGAGGG TTGAAGCAAT CTCAAACGGA TCAACAGCAA 
GTGATGGGTA GTAGCAATTT CACGCTTCAG CTATTTCAAA GAACGCTCGA AGGAACTCTG 
GATTCTTTCC AAAACTCAAT TCATGACGAC GTAAGGAACC TTCACATTGA GATCCTCAGA 
CAGTTCCACA TGCACGAGAT GGAGATGTCG AAGGTGTTAA GCTCGATTCT GGAGAACCAA 
GCGGAGCAAA TGAAGGAGTT AAAGTTGTTG CGAAAAGAGA ATCAAGAGCT CAGACAAAGG 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196216.2
CDS	171..2516
Translation	MMSNLVEPSWRLLAASGGDTVKLFDVSADSGDPCVLSYTPSPGCAVNSVKWNHTNLVVASTGEDKKISLWRKNGQSLGTVPVTGKDGGDSAEECLSAISFSKKGSRYICSGGTGQIVKIWDLQRKLCIKKLKGHTSTITGVMYNCKDEHLASVSVGGDLIVHNLASGARATELKDPNGQVLRLLDYSRSSRHLLVTAGDDGTVHLWDTTGRSPKMSWLKQHSAPTAGVCFSPSNEKIIASVGMDKKLYTYDSGSRRSSSCIAYEAPFSSLAFGDNGYILVAGTSNGRVVFYDIRGKPQPVTVLHAFSNSEDVTSLSWQTSKPVIVNEKNYTSEMALLGSTVEDSVVIPDPLPSTTPSASQSAMAPGSRGVSASTVNASSVEQTPNRNHLWPSGPLGRLHALRANDSYNDDMGVFSPIIDVSSVEKWADSEGYNNKDHLVVDNKPSSLLFPSSSKGYSFGDNGSKEHPIFDWKPSSTSKQDDPRAAFSSFGSITPTASSKSEDSALTPPEAWGGDKFSEKFNQLANEKFSDKFSHLHAPSRLAVSSTGASTSGSMFSSSRDFPLSHGQTNFANASLEFPRIRDFSSTFETSSTQTDNNLPSSPLFTKGITAPGNIDSLRLSPNFTRRFSTYAERISTTSSFSDGASLSLGGSPKIKKTGSETREEVLNHLLARPETVVATEAGAMPLMNGGLKQSQTDQQQVMGSSNFTLQLFQRTLEGTLDSFQNSIHDDVRNLHIEILRQFHMHEMEMSKVLSSILENQAEQMKELKLLRKENQELRQRL

Target ORF information:

RefSeq Version	NM_120679.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (NEDD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120679.3

ORF Insert Sequence:

ATGATGTCGA ACTTGGTAGA GCCATCGTGG CGCCTTCTGG CAGCGAGCGG CGGAGATACG 
GTTAAGCTTT TCGACGTTTC TGCCGATTCC GGCGATCCCT GCGTTTTGAG CTATACTCCG 
TCACCTGGTT GTGCTGTCAA CTCCGTCAAG TGGAATCATA CGAATTTAGT GGTGGCCAGT 
ACGGGAGAAG ATAAGAAGAT ATCCCTGTGG CGGAAAAATG GGCAGAGTTT AGGAACAGTT 
CCAGTTACTG GGAAGGACGG TGGTGATAGT GCTGAGGAAT GTTTATCGGC TATTAGCTTC 
AGCAAAAAAG GCTCCAGGTA CATTTGTTCT GGAGGAACTG GTCAAATTGT TAAGATATGG 
GATCTGCAAA GAAAGCTTTG TATTAAGAAG TTGAAAGGTC ATACAAGTAC AATAACGGGT 
GTGATGTACA ATTGCAAAGA TGAGCATTTG GCTTCTGTTA GTGTTGGTGG GGATCTAATA 
GTCCATAACC TTGCATCTGG AGCAAGGGCT ACCGAACTCA AAGATCCAAA TGGGCAGGTA 
TTAAGGTTGC TTGATTATTC AAGGTCTAGT CGACACCTCC TAGTTACTGC AGGTGATGAT 
GGCACTGTAC ATCTGTGGGA CACAACTGGT CGTAGCCCGA AGATGTCCTG GTTGAAGCAG 
CATTCGGCAC CAACTGCTGG TGTTTGCTTC TCTCCATCAA ACGAAAAGAT AATTGCTAGT 
GTCGGGATGG ACAAAAAGCT TTACACTTAT GATTCTGGAT CCAGAAGATC TTCGTCCTGC 
ATTGCCTATG AGGCACCTTT CTCCTCTTTG GCGTTTGGGG ATAATGGCTA TATTCTGGTA 
GCTGGAACCA GTAACGGTAG AGTAGTGTTT TACGACATCC GTGGAAAACC GCAGCCTGTT 
ACTGTCCTGC ACGCTTTCAG TAATTCAGAG GATGTAACAA GTTTATCATG GCAAACATCA 
AAACCAGTTA TTGTGAATGA GAAAAATTAC ACTTCTGAGA TGGCTCTTCT TGGCAGTACT 
GTGGAAGATT CAGTTGTCAT TCCAGATCCA CTTCCCTCAA CGACACCCTC AGCTTCACAG 
TCCGCTATGG CACCAGGTTC CCGTGGAGTA TCCGCGAGTA CTGTAAATGC ATCATCAGTT 
GAACAAACAC CAAATAGAAA TCATCTATGG CCTAGTGGGC CACTGGGCAG ACTGCATGCA 
CTTCGTGCAA ATGACAGTTA TAATGATGAT ATGGGTGTTT TTTCACCTAT TATTGACGTA 
TCATCTGTTG AGAAGTGGGC TGATAGTGAA GGATATAATA ATAAGGACCA TTTAGTTGTT 
GACAACAAGC CGTCCTCTTT GCTATTTCCG TCATCCAGCA AGGGATATTC GTTTGGAGAC 
AATGGAAGCA AGGAACATCC AATATTCGAT TGGAAACCAA GCTCTACTTC GAAACAGGAT 
GACCCGAGGG CTGCTTTTTC GTCTTTTGGA TCTATTACGC CAACAGCATC ATCCAAAAGT 
GAAGACTCTG CCTTGACACC TCCAGAAGCA TGGGGTGGTG ATAAATTTTC TGAGAAGTTT 
AACCAATTGG CCAATGAGAA ATTTTCTGAT AAATTTAGCC ACCTGCATGC ACCTTCACGT 
CTCGCTGTTT CAAGCACAGG TGCTAGTACA TCTGGATCAA TGTTCTCTAG CTCTCGAGAT 
TTCCCCTTGT CGCATGGTCA GACAAACTTC GCAAACGCTA GCTTAGAATT CCCACGGATC 
AGAGACTTCT CTTCAACCTT TGAGACATCC TCAACACAGA CAGACAACAA CTTGCCTTCA 
AGCCCATTGT TTACAAAAGG CATCACAGCT CCTGGTAACA TTGATTCGTT GAGGCTATCA 
CCAAATTTTA CTCGTAGGTT CTCAACATAT GCTGAGAGAA TCAGCACCAC TTCTTCCTTT 
AGTGACGGGG CCTCTCTCAG TCTGGGTGGC TCACCCAAGA TAAAGAAAAC AGGCTCAGAA 
ACGAGAGAGG AAGTTTTAAA TCATCTGTTG GCAAGACCAG AGACAGTAGT TGCAACAGAA 
GCAGGAGCTA TGCCGCTTAT GAATGGAGGG TTGAAGCAAT CTCAAACGGA TCAACAGCAA 
GTGATGGGTA GTAGCAATTT CACGCTTCAG CTATTTCAAA GAACGCTCGA AGGAACTCTG 
GATTCTTTCC AAAACTCAAT TCATGACGAC GTAAGGAACC TTCACATTGA GATCCTCAGA 
CAGTTCCACA TGCACGAGAT GGAGATGTCG AAGGTGTTAA GCTCGATTCT GGAGAACCAA 
GCGGAGCAAA TGAAGGAGTT AAAGTTGTTG CGAAAAGAGA ATCAAGAGCT CAGACAAAGG 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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NEDD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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