ASA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASA1
Entrez Gene ID	830457
Full Name	anthranilate synthase alpha subunit 1
Synonyms	A-METHYL TRYPTOPHAN RESISTANT 1,AMT1,ANTHRANILATE SYNTHASE ALPHA SUBUNIT,JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1,JDL1,MJJ3.14,MJJ3_14,TRP5,TRYPTOPHAN BIOSYNTHESIS 5,WEAK ETHYLENE INSENSITIVE 2,WEI2,anthranilate synthase alpha subunit 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_120655.4	NP_196192.1	anthranilate synthase alpha subunit 1
NM_001203302.1	NP_001190231.1	anthranilate synthase alpha subunit 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ASA1	NP_001190231.1
Eremothecium gossypii	AGOS_ABR209W	NP_983158.1
Oryza sativa (rice)	Os03g0826500	NP_001051764.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

indole-3-butyric acid induced adventitious root-formation by conversion into Indole-3-acetic acid (IAA) involving nitric oxide activity, and by a positive action on IAA-transport and ASA1/ASB1-mediated IAA-biosynthesis
Title: Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity.

Using loss- and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth.
Title: Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression.

In BnaA.TTG2.a.1-overexpressing Arabidopsis under salt stress, the endogenous indole-3-acetic acid content was reduced, and expression of two auxin biosynthesis genes, TRYPTOPHAN BIOSYNTHESIS 5 (TRP5) and YUCCA2 (YUC2), was downregulated.
Title: Expression of Brassica napus TTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes.

Mutations of ASA1 and the TIR1/AFBs auxin receptor genes impair the inhibitory effect of 5 muM MeJA on PIN2 endocytosis and enhance the reduction effect of 50 muM MeJA on the plasma membrane accumulation of PIN2.
Title: Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein.

Pore mutations in ammonium transporter AMT1 may have a role in increased electrogenic ammonium transport activity
Title: Pore mutations in ammonium transporter AMT1 with increased electrogenic ammonium transport activity.

The following ASA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb17710	NM_120655.4 Latest version!	Arabidopsis thaliana anthranilate synthase alpha subunit 1 (ASA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb29796	NM_001203302.1 Latest version!	Arabidopsis thaliana anthranilate synthase alpha subunit 1 (ASA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASA1 ( NM_120655.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196192.1 Arabidopsis thaliana anthranilate synthase alpha subunit 1 (ASA1), mRNA.

Price & Availability↑

CloneID	OAb17710
Clone ID Related Accession (Same CDS sequence)	NM_120655.4
Accession Version	NM_120655.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	anthranilate synthase alpha subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_120655.3.

ATGTCTTCCT CTATGAACGT AGCGACGATG CAAGCACTGA CTTTCTCTCG CCGGCTTCTC 
CCTTCTGTTG CTTCTCGTTA TCTCTCTTCT TCTTCTGTGA CCGTTACTGG ATACTCCGGT 
AGAAGCTCGG CTTACGCGCC TTCTTTCCGT TCGATTAAAT GCGTCTCTGT TTCTCCGGAA 
GCTTCAATAG TAAGTGATAC AAAGAAGTTG GCAGATGCTT CTAAGAGTAC AAACCTTATA 
CCAATTTACC GCTGTATATT CTCTGATCAG CTTACTCCTG TTCTTGCTTA CCGTTGTTTG 
GTCAAAGAAG ATGACCGTGA AGCTCCTAGC TTTCTTTTCG AGTCCGTTGA GCCTGGTTCT 
CAGATGTCTA GCGTTGGTCG TTATAGCGTT GTTGGGGCTC AGCCTGCGAT GGAGATCGTG 
GCAAAGGAGA ATAAAGTTAT TGTAATGGAT CACAACAATG AAACCATGAC TGAGGAATTC 
GTCGAAGATC CAATGGAGAT CCCAAGAAAA ATCTCTGAGA AATGGAACCC TGATCCTCAA 
CTAGTTCAGG ACCTTCCAGA TGCGTTTTGT GGTGGGTGGG TTGGTTTTTT CTCGTACGAC 
ACTGTTCGTT ATGTTGAGAA GAGGAAATTG CCATTTTCAA AGGCCCCTGA GGATGATAGG 
AACTTGCCAG ACATGCATCT TGGTCTGTAC GACGATGTAG TTGTATTTGA TCACGTGGAA 
AAGAAAGCAT ATGTCATTCA CTGGATTAGA CTAGATGGGA GCCTTCCTTA CGAAAAGGCA 
TACAGTAATG GAATGCAACA TTTGGAGAAC TTGGTGGCCA AGTTACATGA TATTGAGCCG 
CCAAAACTGG CTGCAGGTAA CGTGAATCTT CAGACACGAC AATTTGGGCC ATCTTTGGAT 
AATTCAAACG TGACATGCGA AGAGTACAAG GAGGCTGTGG TCAAGGCCAA AGAACATATA 
CTTGCAGGAG ACATATTTCA GATCGTGCTG AGTCAACGTT TTGAGCGGCG AACATTTGCA 
GACCCCTTTG AAGTTTATAG AGCACTAAGA GTTGTGAATC CAAGTCCGTA TATGGGTTAT 
TTGCAGGCTA GAGGATGCAT TTTGGTAGCA TCAAGTCCAG AAATTCTCAC CAAAGTAAAG 
CAGAACAAGA TAGTGAATCG GCCATTGGCA GGAACCAGCA AGAGAGGGAA GAATGAAGTT 
GAGGATAAGA GATTAGAAAA GGAACTGCTA GAGAATGAAA AGCAATGTGC TGAGCACATC 
ATGTTGGTTG ATCTCGGTCG CAACGATGTT GGAAAGGTTA CGAAATACGG ATCAGTGAAA 
GTAGAGAAGC TTATGAACAT CGAACGTTAT TCCCATGTTA TGCATATAAG CTCCACGGTG 
ACAGGAGAAT TACAAGATGG TTTGACTTGC TGGGACGTAC TACGTGCGGC TTTACCAGTG 
GGAACAGTTA GTGGTGCACC AAAGGTCAAA GCTATGGAAC TAATCGATGA GCTAGAGCCA 
ACGAGGCGTG GACCATACAG TGGCGGTTTT GGTGGAGTCT CCTTCACTGG TGACATGGAC 
ATTGCTTTAT CCCTTAGGAC AATCGTTTTT CCGACAGCAT GTCAATACAA TACAATGTAC 
TCTTACAAGG ATGCTAACAA ACGGCGTGAG TGGGTGGCTT ATCTTCAAGC TGGAGCTGGT 
GTAGTAGCTG ATAGTGACCC GCAAGACGAA CACTGTGAGT GCCAGAACAA AGCCGCTGGT 
CTTGCTCGAG CCATCGACTT GGCTGAATCT GCATTTGTGA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196192.1
CDS	123..1910
Translation	MSSSMNVATMQALTFSRRLLPSVASRYLSSSSVTVTGYSGRSSAYAPSFRSIKCVSVSPEASIVSDTKKLADASKSTNLIPIYRCIFSDQLTPVLAYRCLVKEDDREAPSFLFESVEPGSQMSSVGRYSVVGAQPAMEIVAKENKVIVMDHNNETMTEEFVEDPMEIPRKISEKWNPDPQLVQDLPDAFCGGWVGFFSYDTVRYVEKRKLPFSKAPEDDRNLPDMHLGLYDDVVVFDHVEKKAYVIHWIRLDGSLPYEKAYSNGMQHLENLVAKLHDIEPPKLAAGNVNLQTRQFGPSLDNSNVTCEEYKEAVVKAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRVVNPSPYMGYLQARGCILVASSPEILTKVKQNKIVNRPLAGTSKRGKNEVEDKRLEKELLENEKQCAEHIMLVDLGRNDVGKVTKYGSVKVEKLMNIERYSHVMHISSTVTGELQDGLTCWDVLRAALPVGTVSGAPKVKAMELIDELEPTRRGPYSGGFGGVSFTGDMDIALSLRTIVFPTACQYNTMYSYKDANKRREWVAYLQAGAGVVADSDPQDEHCECQNKAAGLARAIDLAESAFVKK

Target ORF information:

RefSeq Version	NM_120655.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana anthranilate synthase alpha subunit 1 (ASA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120655.4

ORF Insert Sequence:

ATGTCTTCCT CTATGAACGT AGCGACGATG CAAGCACTGA CTTTCTCTCG CCGGCTTCTC 
CCTTCTGTTG CTTCTCGTTA TCTCTCTTCT TCTTCTGTGA CCGTTACTGG ATACTCCGGT 
AGAAGCTCGG CTTACGCGCC TTCTTTCCGT TCGATTAAAT GCGTCTCTGT TTCTCCGGAA 
GCTTCAATAG TAAGTGATAC AAAGAAGTTG GCAGATGCTT CTAAGAGTAC AAACCTTATA 
CCAATTTACC GCTGTATATT CTCTGATCAG CTTACTCCTG TTCTTGCTTA CCGTTGTTTG 
GTCAAAGAAG ATGACCGTGA AGCTCCTAGC TTTCTTTTCG AGTCCGTTGA GCCTGGTTCT 
CAGATGTCTA GCGTTGGTCG TTATAGCGTT GTTGGGGCTC AGCCTGCGAT GGAGATCGTG 
GCAAAGGAGA ATAAAGTTAT TGTAATGGAT CACAACAATG AAACCATGAC TGAGGAATTC 
GTCGAAGATC CAATGGAGAT CCCAAGAAAA ATCTCTGAGA AATGGAACCC TGATCCTCAA 
CTAGTTCAGG ACCTTCCAGA TGCGTTTTGT GGTGGGTGGG TTGGTTTTTT CTCGTACGAC 
ACTGTTCGTT ATGTTGAGAA GAGGAAATTG CCATTTTCAA AGGCCCCTGA GGATGATAGG 
AACTTGCCAG ACATGCATCT TGGTCTGTAC GACGATGTAG TTGTATTTGA TCACGTGGAA 
AAGAAAGCAT ATGTCATTCA CTGGATTAGA CTAGATGGGA GCCTTCCTTA CGAAAAGGCA 
TACAGTAATG GAATGCAACA TTTGGAGAAC TTGGTGGCCA AGTTACATGA TATTGAGCCG 
CCAAAACTGG CTGCAGGTAA CGTGAATCTT CAGACACGAC AATTTGGGCC ATCTTTGGAT 
AATTCAAACG TGACATGCGA AGAGTACAAG GAGGCTGTGG TCAAGGCCAA AGAACATATA 
CTTGCAGGAG ACATATTTCA GATCGTGCTG AGTCAACGTT TTGAGCGGCG AACATTTGCA 
GACCCCTTTG AAGTTTATAG AGCACTAAGA GTTGTGAATC CAAGTCCGTA TATGGGTTAT 
TTGCAGGCTA GAGGATGCAT TTTGGTAGCA TCAAGTCCAG AAATTCTCAC CAAAGTAAAG 
CAGAACAAGA TAGTGAATCG GCCATTGGCA GGAACCAGCA AGAGAGGGAA GAATGAAGTT 
GAGGATAAGA GATTAGAAAA GGAACTGCTA GAGAATGAAA AGCAATGTGC TGAGCACATC 
ATGTTGGTTG ATCTCGGTCG CAACGATGTT GGAAAGGTTA CGAAATACGG ATCAGTGAAA 
GTAGAGAAGC TTATGAACAT CGAACGTTAT TCCCATGTTA TGCATATAAG CTCCACGGTG 
ACAGGAGAAT TACAAGATGG TTTGACTTGC TGGGACGTAC TACGTGCGGC TTTACCAGTG 
GGAACAGTTA GTGGTGCACC AAAGGTCAAA GCTATGGAAC TAATCGATGA GCTAGAGCCA 
ACGAGGCGTG GACCATACAG TGGCGGTTTT GGTGGAGTCT CCTTCACTGG TGACATGGAC 
ATTGCTTTAT CCCTTAGGAC AATCGTTTTT CCGACAGCAT GTCAATACAA TACAATGTAC 
TCTTACAAGG ATGCTAACAA ACGGCGTGAG TGGGTGGCTT ATCTTCAAGC TGGAGCTGGT 
GTAGTAGCTG ATAGTGACCC GCAAGACGAA CACTGTGAGT GCCAGAACAA AGCCGCTGGT 
CTTGCTCGAG CCATCGACTT GGCTGAATCT GCATTTGTGA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASA1 ( NM_001203302.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190231.1 Arabidopsis thaliana anthranilate synthase alpha subunit 1 (ASA1), mRNA.

Price & Availability↑

CloneID	OAb29796
Clone ID Related Accession (Same CDS sequence)	NM_001203302.1
Accession Version	NM_001203302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	anthranilate synthase alpha subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTCTTCCT CTATGAACGT AGCGACGATG CAAGCACTGA CTTTCTCTCG CCGGCTTCTC 
CCTTCTGTTG CTTCTCGTTA TCTCTCTTCT TCTTCTGTGA CCGTTACTGG ATACTCCGGT 
AGAAGCTCGG CTTACGCGCC TTCTTTCCGT TCGATTAAAT GCGTCTCTGT TTCTCCGGAA 
GCTTCAATAG TAAGTGATAC AAAGAAGTTG GCAGATGCTT CTAAGAGTAC AAACCTTATA 
CCAATTTACC GCTGTATATT CTCTGATCAG CTTACTCCTG TTCTTGCTTA CCGTTGTTTG 
GTCAAAGAAG ATGACCGTGA AGCTCCTAGC TTTCTTTTCG AGTCCGTTGA GCCTGGTTCT 
CAGATGTCTA GCGTTGGTCG TTATAGCGTT GTTGGGGCTC AGCCTGCGAT GGAGATCGTG 
GCAAAGGAGA ATAAAGTTAT TGTAATGGAT CACAACAATG AAACCATGAC TGAGGAATTC 
GTCGAAGATC CAATGGAGAT CCCAAGAAAA ATCTCTGAGA AATGGAACCC TGATCCTCAA 
CTAGTTCAGG ACCTTCCAGA TGCGTTTTGT GGTGGGTGGG TTGGTTTTTT CTCGTACGAC 
ACTGTTCGTT ATGTTGAGAA GAGGAAATTG CCATTTTCAA AGGCCCCTGA GGATGATAGG 
AACTTGCCAG ACATGCATCT TGGTCTGTAC GACGATGTAG TTGTATTTGA TCACGTGGAA 
AAGAAAGCAT ATGTCATTCA CTGGATTAGA CTAGATGGGA GCCTTCCTTA CGAAAAGGCA 
TACAGTAATG GAATGCAACA TTTGGAGAAC TTGGTGGCCA AGTTACATGA TATTGAGCCG 
CCAAAACTGG CTGCAGGTAA CGTGAATCTT CAGACACGAC AATTTGGGCC ATCTTTGGAT 
AATTCAAACG TGACATGCGA AGAGTACAAG GAGGCTGTGG TCAAGGCCAA AGAACATATA 
CTTGCAGGAG ACATATTTCA GATCGTGCTG AGTCAACGTT TTGAGCGGCG AACATTTGCA 
GACCCCTTTG AAGTTTATAG AGCACTAAGA GTTGTGAATC CAAGTCCGTA TATGGGTTAT 
TTGCAGGTTG GTGATGGAGA GAACATTTCT CAACAACTTT GCATTTTGTA CTTGTGGATA 
AACCATCAAT GCTGTTTTCG TTCTGTTATT CAGGCTAGAG GATGCATTTT GGTAGCATCA 
AGTCCAGAAA TTCTCACCAA AGTAAAGCAG AACAAGATAG TGAATCGGCC ATTGGCAGGA 
ACCAGCAAGA GAGGGAAGAA TGAAGTTGAG GATAAGAGAT TAGAAAAGGA ACTGCTAGAG 
AATGAAAAGC AATGTGCTGA GCACATCATG TTGGTTGATC TCGGTCGCAA CGATGTTGGA 
AAGGTTACGA AATACGGATC AGTGAAAGTA GAGAAGCTTA TGAACATCGA ACGTTATTCC 
CATGTTATGC ATATAAGCTC CACGGTGACA GGAGAATTAC AAGATGGTTT GACTTGCTGG 
GACGTACTAC GTGCGGCTTT ACCAGTGGGA ACAGTTAGTG GTGCACCAAA GGTCAAAGCT 
ATGGAACTAA TCGATGAGCT AGAGCCAACG AGGCGTGGAC CATACAGTGG CGGTTTTGGT 
GGAGTCTCCT TCACTGGTGA CATGGACATT GCTTTATCCC TTAGGACAAT CGTTTTTCCG 
ACAGCATGTC AATACAATAC AATGTACTCT TACAAGGATG CTAACAAACG GCGTGAGTGG 
GTGGCTTATC TTCAAGCTGG AGCTGGTGTA GTAGCTGATA GTGACCCGCA AGACGAACAC 
TGTGAGTGCC AGAACAAAGC CGCTGGTCTT GCTCGAGCCA TCGACTTGGC TGAATCTGCA 
TTTGTGAAAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190231.1
CDS	100..1974
Translation	MSSSMNVATMQALTFSRRLLPSVASRYLSSSSVTVTGYSGRSSAYAPSFRSIKCVSVSPEASIVSDTKKLADASKSTNLIPIYRCIFSDQLTPVLAYRCLVKEDDREAPSFLFESVEPGSQMSSVGRYSVVGAQPAMEIVAKENKVIVMDHNNETMTEEFVEDPMEIPRKISEKWNPDPQLVQDLPDAFCGGWVGFFSYDTVRYVEKRKLPFSKAPEDDRNLPDMHLGLYDDVVVFDHVEKKAYVIHWIRLDGSLPYEKAYSNGMQHLENLVAKLHDIEPPKLAAGNVNLQTRQFGPSLDNSNVTCEEYKEAVVKAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRVVNPSPYMGYLQVGDGENISQQLCILYLWINHQCCFRSVIQARGCILVASSPEILTKVKQNKIVNRPLAGTSKRGKNEVEDKRLEKELLENEKQCAEHIMLVDLGRNDVGKVTKYGSVKVEKLMNIERYSHVMHISSTVTGELQDGLTCWDVLRAALPVGTVSGAPKVKAMELIDELEPTRRGPYSGGFGGVSFTGDMDIALSLRTIVFPTACQYNTMYSYKDANKRREWVAYLQAGAGVVADSDPQDEHCECQNKAAGLARAIDLAESAFVKK

Target ORF information:

RefSeq Version	NM_001203302.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana anthranilate synthase alpha subunit 1 (ASA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203302.1

ORF Insert Sequence:

ATGTCTTCCT CTATGAACGT AGCGACGATG CAAGCACTGA CTTTCTCTCG CCGGCTTCTC 
CCTTCTGTTG CTTCTCGTTA TCTCTCTTCT TCTTCTGTGA CCGTTACTGG ATACTCCGGT 
AGAAGCTCGG CTTACGCGCC TTCTTTCCGT TCGATTAAAT GCGTCTCTGT TTCTCCGGAA 
GCTTCAATAG TAAGTGATAC AAAGAAGTTG GCAGATGCTT CTAAGAGTAC AAACCTTATA 
CCAATTTACC GCTGTATATT CTCTGATCAG CTTACTCCTG TTCTTGCTTA CCGTTGTTTG 
GTCAAAGAAG ATGACCGTGA AGCTCCTAGC TTTCTTTTCG AGTCCGTTGA GCCTGGTTCT 
CAGATGTCTA GCGTTGGTCG TTATAGCGTT GTTGGGGCTC AGCCTGCGAT GGAGATCGTG 
GCAAAGGAGA ATAAAGTTAT TGTAATGGAT CACAACAATG AAACCATGAC TGAGGAATTC 
GTCGAAGATC CAATGGAGAT CCCAAGAAAA ATCTCTGAGA AATGGAACCC TGATCCTCAA 
CTAGTTCAGG ACCTTCCAGA TGCGTTTTGT GGTGGGTGGG TTGGTTTTTT CTCGTACGAC 
ACTGTTCGTT ATGTTGAGAA GAGGAAATTG CCATTTTCAA AGGCCCCTGA GGATGATAGG 
AACTTGCCAG ACATGCATCT TGGTCTGTAC GACGATGTAG TTGTATTTGA TCACGTGGAA 
AAGAAAGCAT ATGTCATTCA CTGGATTAGA CTAGATGGGA GCCTTCCTTA CGAAAAGGCA 
TACAGTAATG GAATGCAACA TTTGGAGAAC TTGGTGGCCA AGTTACATGA TATTGAGCCG 
CCAAAACTGG CTGCAGGTAA CGTGAATCTT CAGACACGAC AATTTGGGCC ATCTTTGGAT 
AATTCAAACG TGACATGCGA AGAGTACAAG GAGGCTGTGG TCAAGGCCAA AGAACATATA 
CTTGCAGGAG ACATATTTCA GATCGTGCTG AGTCAACGTT TTGAGCGGCG AACATTTGCA 
GACCCCTTTG AAGTTTATAG AGCACTAAGA GTTGTGAATC CAAGTCCGTA TATGGGTTAT 
TTGCAGGTTG GTGATGGAGA GAACATTTCT CAACAACTTT GCATTTTGTA CTTGTGGATA 
AACCATCAAT GCTGTTTTCG TTCTGTTATT CAGGCTAGAG GATGCATTTT GGTAGCATCA 
AGTCCAGAAA TTCTCACCAA AGTAAAGCAG AACAAGATAG TGAATCGGCC ATTGGCAGGA 
ACCAGCAAGA GAGGGAAGAA TGAAGTTGAG GATAAGAGAT TAGAAAAGGA ACTGCTAGAG 
AATGAAAAGC AATGTGCTGA GCACATCATG TTGGTTGATC TCGGTCGCAA CGATGTTGGA 
AAGGTTACGA AATACGGATC AGTGAAAGTA GAGAAGCTTA TGAACATCGA ACGTTATTCC 
CATGTTATGC ATATAAGCTC CACGGTGACA GGAGAATTAC AAGATGGTTT GACTTGCTGG 
GACGTACTAC GTGCGGCTTT ACCAGTGGGA ACAGTTAGTG GTGCACCAAA GGTCAAAGCT 
ATGGAACTAA TCGATGAGCT AGAGCCAACG AGGCGTGGAC CATACAGTGG CGGTTTTGGT 
GGAGTCTCCT TCACTGGTGA CATGGACATT GCTTTATCCC TTAGGACAAT CGTTTTTCCG 
ACAGCATGTC AATACAATAC AATGTACTCT TACAAGGATG CTAACAAACG GCGTGAGTGG 
GTGGCTTATC TTCAAGCTGG AGCTGGTGTA GTAGCTGATA GTGACCCGCA AGACGAACAC 
TGTGAGTGCC AGAACAAAGC CGCTGGTCTT GCTCGAGCCA TCGACTTGGC TGAATCTGCA 
TTTGTGAAAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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ASA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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