ACD2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACD2
Entrez Gene ID	829854
Full Name	accelerated cell death 2 (ACD2)
Synonyms	ACCELERATED CELL DEATH 2,AP22.36,AP22_36,ARABIDOPSIS THALIANA RED CHLOROPHYLL CATABOLITE REDUCTASE,ATRCCR,RCCR,RED CHLOROPHYLL CATABOLITE REDUCTASE
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119863.4	NP_195417.1	accelerated cell death 2 (ACD2)

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os10g0389200	NP_001064500.1
Arabidopsis thaliana (thale cress)	ACD2	NP_195417.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACD2 localizes dynamically during infection to protect cells from pro-death mobile substrate molecules, some of which may originate in chloroplasts, but have major effects on mitochondria.
Title: Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis.

Two conserved acidic residues, Glu154 and Asp291, of chlorophyll catabolite reductase sandwich the C20/C1 double bond of red chlorophyll catabolite, suggesting that these two residues are involved in site-specific reduction.
Title: Crystal structures of the substrate-bound forms of red chlorophyll catabolite reductase: implications for site-specific and stereospecific reaction.

crystal structure of RCCR at 2.4 A resolution shows RCCR forms a homodimer, in which each subunit folds in an alpha/beta/alpha sandwich.
Title: Crystal structure of red chlorophyll catabolite reductase: enlargement of the ferredoxin-dependent bilin reductase family.

RCCR (At4g37000), together with pheophorbide a oxygenase, is required for the detoxification of chlorophyll catabolites.
Title: In vivo participation of red chlorophyll catabolite reductase in chlorophyll breakdown.

The Arabidopsis thaliana chloroplast protein ACCELERATED CELL DEATH2 (ACD2) modulates the amount of programmed cell death (PCD) triggered by Pseudomonas syringae and protoporphyrin IX (PPIX) treatment.
Title: Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death.

The following ACD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb17298	NM_119863.4 Latest version!	Arabidopsis thaliana accelerated cell death 2 (ACD2) (ACD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACD2 ( NM_119863.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195417.1 Arabidopsis thaliana accelerated cell death 2 (ACD2) (ACD2), mRNA.

Price & Availability↑

CloneID	OAb17298
Clone ID Related Accession (Same CDS sequence)	NM_119863.4
Accession Version	NM_119863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	accelerated cell death 2 (ACD2)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119863.3.

ATGGCGATGA TATTTTGCAA CACTCTCTAC TCTTCTTCTT CTCCATCATA TCTCTCGCCG 
TTAACTTCAA AACCGTCGCG ATTCTCAAAG AATCTCAGAC CTCGAGCTCA ATTCCAGTCC 
ATGGAAGACC ACGACGATCA CCTCCGCCGA AAATTTATGG AGTTCCCGTA TGTGTCACCC 
ACGCGGAAGC AGCTCATGGT TGATCTCATG TCGACGGTGG AGAATCGCCT CCAATCACAA 
CTCCTTCCCT GTAACCTCCC TCCAGATGTA CGAAACTTCA ATAACCCTAA CGGTTCCGCC 
GAAGCATCTC TTCATATCAG ATCCGGCGAC AAATCTTCTC CGATTGATTT TGTTATAGGA 
AGTTGGATAC ATTGCAAGAT CCCAACAGGA GTATCTTTGA ATATAACAAG CATCTCTGGA 
TTCTTAAACT CATCAACAAA AGCTCCAAAC TTTGTGGTCG AACTAATACA GAGCAGTTCC 
AAGTCGCTTG TGCTAATCCT TGACCTCCCA CATCGTAAAG ATCTTGTTCT TAACCCGGAT 
TATCTCAAGG AGTATTACCA AGACACTGCT CTTGATTCTC ATCGACAATC TCTCCTTAAG 
CTACCTGAAG TTAACCCTTA TGTGTCTCCT TCTCTCTTTG TCCGTTCTGC TTTCTCTCCT 
ACTGCTTCGA TGCTTAAGAT TGATGCGGAG GAAGAGGATA AGTTGGAGGA GATATTGAGA 
GATCATGTTA GTCCAGCTGC TAAGGAGGTT CTCGAGGTTT GGTTGGAGCG GTGTGTGAAG 
GAAGAAGAAG AGAAGATTGT GGTTGGGGAA GAAGAGAGAA TGGAGTTGGA GAGAAGAGAT 
AAAAGCTTTA GAAGGAAGAG CATAGAGGAC GATTTGGATT TGCAGTTTCC GAGAATGTTT 
GGTGAAGAAG TTTCCTCCCG TGTTGTACAC GCTATTAAAG AAGCTTTCGG TGTTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195417.1
CDS	16..975
Translation	MAMIFCNTLYSSSSPSYLSPLTSKPSRFSKNLRPRAQFQSMEDHDDHLRRKFMEFPYVSPTRKQLMVDLMSTVENRLQSQLLPCNLPPDVRNFNNPNGSAEASLHIRSGDKSSPIDFVIGSWIHCKIPTGVSLNITSISGFLNSSTKAPNFVVELIQSSSKSLVLILDLPHRKDLVLNPDYLKEYYQDTALDSHRQSLLKLPEVNPYVSPSLFVRSAFSPTASMLKIDAEEEDKLEEILRDHVSPAAKEVLEVWLERCVKEEEEKIVVGEEERMELERRDKSFRRKSIEDDLDLQFPRMFGEEVSSRVVHAIKEAFGVL

Target ORF information:

RefSeq Version	NM_119863.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana accelerated cell death 2 (ACD2) (ACD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119863.4

ORF Insert Sequence:

ATGGCGATGA TATTTTGCAA CACTCTCTAC TCTTCTTCTT CTCCATCATA TCTCTCGCCG 
TTAACTTCAA AACCGTCGCG ATTCTCAAAG AATCTCAGAC CTCGAGCTCA ATTCCAGTCC 
ATGGAAGACC ACGACGATCA CCTCCGCCGA AAATTTATGG AGTTCCCGTA TGTGTCACCC 
ACGCGGAAGC AGCTCATGGT TGATCTCATG TCGACGGTGG AGAATCGCCT CCAATCACAA 
CTCCTTCCCT GTAACCTCCC TCCAGATGTA CGAAACTTCA ATAACCCTAA CGGTTCCGCC 
GAAGCATCTC TTCATATCAG ATCCGGCGAC AAATCTTCTC CGATTGATTT TGTTATAGGA 
AGTTGGATAC ATTGCAAGAT CCCAACAGGA GTATCTTTGA ATATAACAAG CATCTCTGGA 
TTCTTAAACT CATCAACAAA AGCTCCAAAC TTTGTGGTCG AACTAATACA GAGCAGTTCC 
AAGTCGCTTG TGCTAATCCT TGACCTCCCA CATCGTAAAG ATCTTGTTCT TAACCCGGAT 
TATCTCAAGG AGTATTACCA AGACACTGCT CTTGATTCTC ATCGACAATC TCTCCTTAAG 
CTACCTGAAG TTAACCCTTA TGTGTCTCCT TCTCTCTTTG TCCGTTCTGC TTTCTCTCCT 
ACTGCTTCGA TGCTTAAGAT TGATGCGGAG GAAGAGGATA AGTTGGAGGA GATATTGAGA 
GATCATGTTA GTCCAGCTGC TAAGGAGGTT CTCGAGGTTT GGTTGGAGCG GTGTGTGAAG 
GAAGAAGAAG AGAAGATTGT GGTTGGGGAA GAAGAGAGAA TGGAGTTGGA GAGAAGAGAT 
AAAAGCTTTA GAAGGAAGAG CATAGAGGAC GATTTGGATT TGCAGTTTCC GAGAATGTTT 
GGTGAAGAAG TTTCCTCCCG TGTTGTACAC GCTATTAAAG AAGCTTTCGG TGTTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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ACD2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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