DCAF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCAF1
Entrez Gene ID	829244
Full Name	DDB1-CUL4 associated factor 1
Synonyms	DDB1-CUL4 associated factor 1,F6E21.80,F6E21_80
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119266.7	NP_194845.4	DDB1-CUL4 associated factor 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DCAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16826	NM_119266.7 Latest version!	Arabidopsis thaliana DDB1-CUL4 associated factor 1 (DCAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCAF1 ( NM_119266.7 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194845.4 Arabidopsis thaliana DDB1-CUL4 associated factor 1 (DCAF1), mRNA.

Price & Availability↑

CloneID	OAb16826
Clone ID Related Accession (Same CDS sequence)	NM_119266.7
Accession Version	NM_119266.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DDB1-CUL4 associated factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119266.6.

ATGGACGGGC AAGAGCATGC CGAGGTCCCT AATTCGATGG TTGAGGATGA CCAATCAGTT 
GTTGCGGCGG AGGCGATTGC AGAGTTGGCG AATTCAACCG GCGAACCTAA TCCTGAAGAA 
GGAGAAGAAC AGAGTGTTGA AGACGAGCTT ATAGCGAAGG CGCAGAAGCT TATGGAAGAT 
ATTACTTCCG TTGCCAATAA CCCTAATCCT AATATCCTTC ACGCTCTTAG TCAGCTTCTC 
GAATCTCAGG AGTCTCTGTT CTTAGAGGAG AATGGGCATT TCTCCAATGC TCGTGGTAGT 
CATAACAGTG GCAAGCTGTG TATTCTCATC CGAGAAAACG ATGAGTTTTT TGAATTGATA 
TCGTCGACTT TTCTATCTGA GAATTCTTAC TCAACCGCAG TAAAGGCAGC CTCTGCAAGG 
TTGCTAATGA ACTGTTCGCT TACGTGGATG TATCCTCATG TTTTCGACGA TGCTGTAACG 
GAAAACTTTA AAAATTGGGT TATGGAAGAG GCAGTCAAGT TTCCTGGTGA AGACTCTGCC 
AAGAAGGAAG CCTCAGATTT TGAAATGTTA AAAACTTATT CTACAGGACT TCTTGCTCTC 
TCTCTGGCGA GTCGTGGGCA AATAGTTGAA GACGTTTTGA CATCAGGACT ATCTGCTAAG 
CTTATGCATT ATCTTCGAGT ACGAGTTCTT AAAGAGCCAA GCACAAGCCG TATACACACA 
ACTGAGACTA AGCACGTCTC CTTAAAAACC AAAGAAGAAG GTCGAAGTAG GGTGCGTAAG 
ATTGTGGATA CAGTTGAAGG TGACCATGTT CTTGAGACGG ACTCCGGAAG AGAGATGGGA 
CAAACTGATG TACAGCCTGA TGGCGAATTC GAAATCGATG GCAGAGATGT ATTTAATGTT 
TCAGGTGTTG TTGATTGTAA AATTAAACCT GGAGATGACA ATAGTGTTAG AGATGACCCT 
TCAAGACACA GACTGAACCG ATCAAAATCA AGGGGGAGAG GAAGGGTTCA CGAAGGTGCA 
CCTGATACAG AAGTTCTCTT GGCATCTCCT AGATTAGGTC GTCTACTTGT CAGAGATAGG 
GATCTATCAA AAATTTCAGA TGGCAGAAAT GCAGAAGACG TGACCGTATG TCTTGGGAAA 
ATGAAGTCTG GTATTATGGA GATAGAAAGA GAGGATAACG ATGAGTGCTT TCAAGGTTGC 
ATAATAGGAA CTAAAAACAT AACTGATCTA GTAAAGAGAG CCGTTGGAGC TGCTGAAACC 
GAAGCTAGAG CTGCCCATGC TCCTGATGAC GCAGCTAAAG CAGCTGGCGA TGCTGCTGCA 
GAGCTTGTTA AAACTGCTGC TTTGGAGGAA TTCAAGTCTT CTGGCAGTGA AGAAGCTGCT 
GTGTCTGCTG CTACTCGAGC AGCTATTACA GTCATAGATG CCGCAGAGGT TTCAAGAAAT 
CCTACTTGTG TCACATCTGA TCAGACAACG GATGTTAGTG AAGTTTCCCT TCCAGATATC 
GAATCATTGG CTCAGTTACA AGAAAAATAT TGCATCCAGT GCCTTGAGAT ACTGGGAGAA 
TATGTTGAGG TTCTCGGGCC TGTACTCCAT GAAAAAGGTG TTGATGTATG CATTGTGCTG 
CTGGAACGTA CATCCCAGTT AGATGATCGT TCGACAGTGT CTCCTTTGTT ACCTGATGTC 
ATGAAGTTAA TTTGCGCTTT GGCTGCGCAC CGGAAGTTTG CAGCAATGTT TGTTGAACGG 
CGTGGCATAC TAAAATTACT TGCTGTTCCA AGGGTCTCTG AGACTTTTTA TGGTCTCTCA 
TCTTGCTTAT ACACCATTGG CTCTCTTCAG GGTATAATGG AGCGTGTCTG TGCACTTCCA 
TTGGTTGTCA TACATCAAGT TGTTAAATTA GCTATCGAAC TTCTCGACTG TTCCCAGGAT 
CAAGCCAGAA AAAATTCAGC TTTATTCTTT GCTGCTGCTT TTGTCTTCAG AGCCATTTTA 
GATGCATTTG ATGCTCAGGA TAGTTTGCAG AAACTCCTTG CAATTCTTAA AGATGCAGCC 
TCAGTTAGAA CTGGTGCTAA TACTGACCGA TCAGCTCCTG AAGTCATGAC GTCCTCAGAG 
AAGCAGATGG CTTTTCACAC CTGTTTTGCT TTACGCCAGT ATTTTAGAGC TCATCTCCTT 
TTGCTTGTTG ATTCAATCCG TCCTAGCAGA ATTAGCCGGG GTGGTGTGCC ATCTTCAATG 
AAGCCTAATA TAAGGGCAGC CTATAAGCCC CTTGACATCA GCAATGAAGC TGTTGATGCC 
ATCTTCCTTC AGTTACAGAA GGATAGGAGG TTGGGTCCGA CCTTTGTGAA AGCTCAGTGG 
CCTGCTGTCA ATAATTTTTT GGCCTCCTCT GGACATGTTA CCATGTTGGA ACTTTGTCAG 
ACTCCACCAG TAGATCGTTA TCTGCATGAT CTTCTTCAGT ATGCTTTTGG GGTTCTTCAC 
ATAGTTACAT CAATACCTGA TGGCCGCAAA GCAATTGCAC ATGCCACACT CAGCAACAAT 
CGTGCTGGCA TTGCTGTCAT TCTGGATGCA GCAAATATTT CCAACAGCAT AGTAGACCCT 
GAGATCATCC AGCCTGCACT AAATGTCCTA ATCAATCTGG TGTGCCCACC ACCATCATTA 
AGCAATAAGC CGCCCCTTGC ACAAAATCAT CAACCTGTTC CTGGCCAAGC CACTACCCGT 
CCCTCTACTG ATGTTGCTGT AGGTACTCAG TCCACTGGTA ATGCTCCGCA AACTCCTGTT 
GCACCAGCAT CTTCAGGTTT GGTTGGGGAT CGGAGAATTT TCTTAGGAGC AGGAACTGGT 
TCTGCTGGCC TTGCTGCTAA GTTGGAGCAG GTTTATCGTC AAGCCCGTGA AGCTGTTCGT 
GGGAATGATG GTATAAAAAT TCTTTTAAAA CTTCTTCAGC CCAGAATATA TGTAAATCCT 
CCTGCTACCC CAGATTGTCT ACGAGCGCTA GCTTGCAGGG TACTTCTTGG TCTGGCTAGA 
GATGATACAA TTGCACAAAT ACTGACTAAA CTTGAGGTTG GTAAGAGTTT ATCAGAACTA 
ATTCGGGATT CAGGTGGTCA GTCAAGTGGC ACAGATCAGG GGAGATGGCA GGCTGAACTT 
GCTCAGGTGG CGCTTGAGCT AATAGGGATT GTGACAAATT CAGGGCATGC AACTACACTA 
ACAGCCAGTG ATGCTGCTAC TCCAACACTG AGGCGCATTG AAAGAGCTGC CATTGCAGCA 
GCAACGCCTA TAACTTATGA TTCTAAGGAG CTTTTACTGC TTATCCATGA GCATCTCCAG 
GCATCAGGTC TTGGTGATAC AGCTTCAGCA CTGTTAAAAG AGGCTCAATT AACTCCTCTA 
CCTTCATCGG CTTCCCCTTC TTCTATTGCA TATTCCACTA CACAGGAGAT GTCTACGCCG 
CTAGCTCAGG AACAATGGCC CTCTGGCCGT GCTAACAGTG GATTTTTCAC CAGCAAACCC 
AAGGTCTGTG CACATGACGA AGATCCAAAT TCTAGATCTA ATGCAGCTCT ATCTGCCAAG 
AAGAAGCATT TGGCTTCCTC GACACTGGAG ATGCCCACAC CAGTAGCACA GCAACAATGG 
CCATCAGGCC GTGCTAACTG TGGTTTTTGT CCCAGCATAC CCAAAATCAA TGCACGTGAT 
GAAGATCCTA GTTCGAGAGG TAACGCAGCT CCATCTGCAA AGAAGAAACA GTTGACTTTC 
TCACCCAGTT TTAGTTCGCA GTCAAGAAAA CAATCTTTCT CCCATGACGC TCTACCTCAG 
TCTACACAGA GAATAAATTG TTGTTCGAAT TCAGATCCTG CTTTGGCAGA TACATCAGAA 
ACTGCTGCAG AATTAGTTTT GAAGAACGAC CTTGATGCCG ATGCTCAATT TAAGACCCCG 
ATTTCCTTTC CAAGAAAACG GAAGTTGTCT GAGCTTAGAG ACTCGTCAGT TCCGGGTAAA 
AGAATCGATT TGGGTGAGCG AAGGAATTCA ACCTTTGCGG ATGGCTCAGG TTTGCAAACA 
CCAGCTTCAG CTTTAGATGC CAACCAATCT GGGAGTTCCA GACTGGGCCA AATGACACCT 
GCTTCTCAGC TACGGCTTCC AAGTGATCCC CAGCCTTCAA ATCCAGAACG GTTATCACTA 
GACTCCCTTG TGGTTCAGTA TCTAAAGCAC CAACACAGGC AGTGTCTGGC TCCAATCACA 
ACTCTTCCCC CTGTGTCTCT CCTACATCCA CATGTCTGTC CTGAACCTAA ACGGTTACTT 
GAAGCTCCAC TAAACATGAC TGGCCGTCTT GGGACCCGTG AGCTTCAAAG TTTCTACAGT 
GGAGTCCATG GGAATCGCAG GGATCGTCAG TTTGTTTTCA GCAGATTCAA ATCTTGGAGA 
TCTTTCCGGG ATGAGACTGC TCTCTTTACA TGCATTGCAC TTCTTGGCGG TACTAATCAT 
ATAGCAGTCG GTAGTCATGC TGGAGAAATC AAGATATTTG AAGCTAGCAG CGGTAGCATG 
CTTGAGAGTG TCTCAGGTCA CCAGGCTCCG GTTACACTTG TTCAGCCGTA TGTCTCTCGT 
GATACTCAGT TGTTACTTTC TTCGAGCTCC AGTGATGTAC AGTTGTGGGA CGCGTCTTCT 
ATAACTGGTG GGCCTAGACA CTCCTTTGAT GGATGCAAGG CTGCAAAATT CAGCAACTCT 
GGGCTACAAT TTGCAGCGCT TTCTTGTGAA GCATCTAGAA AAGATGTTCT TCTATATGAC 
GTACAGACCT GCAGCCCTTG CCAAAAACTC ACTGACACAG TCACATCCTC TCGAAGTAAT 
CCCTATTCTC TTGTGCACTT CAGCCCTTGC GATACACTGA TTTTGTGGAA TGGTGTTCTC 
TGGGACCGCC GTATCCCCGA GAAGGTCAGA CGGTTTGATC AATTTACTGA TTATGGCGGA 
GGTGGGTTTC ATCCTTCTAG GAACGAGGTG ATCATAAACT CAGAGATCTG GGACATGAGG 
ACTTTCAAAC TTCTTCGGAG TGTACCATCA CTGGACCAGA CAGCTATTAC GTTCAACTCT 
AGGGGAGATG TTATATACGC AATGCTGAGG AGAAACATCG AGGACGTTAT GTCAGCGGTT 
CACACACGCC GTGTGAAACA TCCCTTGTTT GCTGCTTTCC GCACTCTTGA TGCGATCAAC 
TACTCAGACA TTGCCACTAT ACCTGTAGAC CGGTGTCTTC TTGACTTTGC CACAGAACCA 
ACAGATTCGT TCCTTGGGCT GATCACAATG GAAGACCAAG AAGATATGTT TTCCTCGGCC 
AGGATGTATG AGATTGGTAG AAGGAGACCA ACAGATGATG ACTCGGATCC CGATGATGAT 
GATGAGACGG AAGATGAAGA TGAAGATGAT GAAGAAGAAG ATGACCTGGA CCGAATTCTG 
GGACTAGCTG GAGACAATAG CGATAGTGGG GATGATGACC TTAGCAGCGA GGACAATGAA 
GATAGTGTGA GCGATTTTGA TGAAGAAGCA GATATTCTCA TTGACGGTGA CTTTATGGAG 
GAGCTTATAG AAGGTGAAAA TGAAGACGAT GGTAATGGTG AGGATGAGGA TGATGATGAC 
GACGGTGAGA TGCAGGATTT CATGAGCAGT GGGGAAGAGG ATGATTATAG AGACAACATT 
CGTTCTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194845.4
CDS	309..5960
Translation	MDGQEHAEVPNSMVEDDQSVVAAEAIAELANSTGEPNPEEGEEQSVEDELIAKAQKLMEDITSVANNPNPNILHALSQLLESQESLFLEENGHFSNARGSHNSGKLCILIRENDEFFELISSTFLSENSYSTAVKAASARLLMNCSLTWMYPHVFDDAVTENFKNWVMEEAVKFPGEDSAKKEASDFEMLKTYSTGLLALSLASRGQIVEDVLTSGLSAKLMHYLRVRVLKEPSTSRIHTTETKHVSLKTKEEGRSRVRKIVDTVEGDHVLETDSGREMGQTDVQPDGEFEIDGRDVFNVSGVVDCKIKPGDDNSVRDDPSRHRLNRSKSRGRGRVHEGAPDTEVLLASPRLGRLLVRDRDLSKISDGRNAEDVTVCLGKMKSGIMEIEREDNDECFQGCIIGTKNITDLVKRAVGAAETEARAAHAPDDAAKAAGDAAAELVKTAALEEFKSSGSEEAAVSAATRAAITVIDAAEVSRNPTCVTSDQTTDVSEVSLPDIESLAQLQEKYCIQCLEILGEYVEVLGPVLHEKGVDVCIVLLERTSQLDDRSTVSPLLPDVMKLICALAAHRKFAAMFVERRGILKLLAVPRVSETFYGLSSCLYTIGSLQGIMERVCALPLVVIHQVVKLAIELLDCSQDQARKNSALFFAAAFVFRAILDAFDAQDSLQKLLAILKDAASVRTGANTDRSAPEVMTSSEKQMAFHTCFALRQYFRAHLLLLVDSIRPSRISRGGVPSSMKPNIRAAYKPLDISNEAVDAIFLQLQKDRRLGPTFVKAQWPAVNNFLASSGHVTMLELCQTPPVDRYLHDLLQYAFGVLHIVTSIPDGRKAIAHATLSNNRAGIAVILDAANISNSIVDPEIIQPALNVLINLVCPPPSLSNKPPLAQNHQPVPGQATTRPSTDVAVGTQSTGNAPQTPVAPASSGLVGDRRIFLGAGTGSAGLAAKLEQVYRQAREAVRGNDGIKILLKLLQPRIYVNPPATPDCLRALACRVLLGLARDDTIAQILTKLEVGKSLSELIRDSGGQSSGTDQGRWQAELAQVALELIGIVTNSGHATTLTASDAATPTLRRIERAAIAAATPITYDSKELLLLIHEHLQASGLGDTASALLKEAQLTPLPSSASPSSIAYSTTQEMSTPLAQEQWPSGRANSGFFTSKPKVCAHDEDPNSRSNAALSAKKKHLASSTLEMPTPVAQQQWPSGRANCGFCPSIPKINARDEDPSSRGNAAPSAKKKQLTFSPSFSSQSRKQSFSHDALPQSTQRINCCSNSDPALADTSETAAELVLKNDLDADAQFKTPISFPRKRKLSELRDSSVPGKRIDLGERRNSTFADGSGLQTPASALDANQSGSSRLGQMTPASQLRLPSDPQPSNPERLSLDSLVVQYLKHQHRQCLAPITTLPPVSLLHPHVCPEPKRLLEAPLNMTGRLGTRELQSFYSGVHGNRRDRQFVFSRFKSWRSFRDETALFTCIALLGGTNHIAVGSHAGEIKIFEASSGSMLESVSGHQAPVTLVQPYVSRDTQLLLSSSSSDVQLWDASSITGGPRHSFDGCKAAKFSNSGLQFAALSCEASRKDVLLYDVQTCSPCQKLTDTVTSSRSNPYSLVHFSPCDTLILWNGVLWDRRIPEKVRRFDQFTDYGGGGFHPSRNEVIINSEIWDMRTFKLLRSVPSLDQTAITFNSRGDVIYAMLRRNIEDVMSAVHTRRVKHPLFAAFRTLDAINYSDIATIPVDRCLLDFATEPTDSFLGLITMEDQEDMFSSARMYEIGRRRPTDDDSDPDDDDETEDEDEDDEEEDDLDRILGLAGDNSDSGDDDLSSEDNEDSVSDFDEEADILIDGDFMEELIEGENEDDGNGEDEDDDDDGEMQDFMSSGEEDDYRDNIRSS

Target ORF information:

RefSeq Version	NM_119266.7
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DDB1-CUL4 associated factor 1 (DCAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119266.7

ORF Insert Sequence:

ATGGACGGGC AAGAGCATGC CGAGGTCCCT AATTCGATGG TTGAGGATGA CCAATCAGTT 
GTTGCGGCGG AGGCGATTGC AGAGTTGGCG AATTCAACCG GCGAACCTAA TCCTGAAGAA 
GGAGAAGAAC AGAGTGTTGA AGACGAGCTT ATAGCGAAGG CGCAGAAGCT TATGGAAGAT 
ATTACTTCCG TTGCCAATAA CCCTAATCCT AATATCCTTC ACGCTCTTAG TCAGCTTCTC 
GAATCTCAGG AGTCTCTGTT CTTAGAGGAG AATGGGCATT TCTCCAATGC TCGTGGTAGT 
CATAACAGTG GCAAGCTGTG TATTCTCATC CGAGAAAACG ATGAGTTTTT TGAATTGATA 
TCGTCGACTT TTCTATCTGA GAATTCTTAC TCAACCGCAG TAAAGGCAGC CTCTGCAAGG 
TTGCTAATGA ACTGTTCGCT TACGTGGATG TATCCTCATG TTTTCGACGA TGCTGTAACG 
GAAAACTTTA AAAATTGGGT TATGGAAGAG GCAGTCAAGT TTCCTGGTGA AGACTCTGCC 
AAGAAGGAAG CCTCAGATTT TGAAATGTTA AAAACTTATT CTACAGGACT TCTTGCTCTC 
TCTCTGGCGA GTCGTGGGCA AATAGTTGAA GACGTTTTGA CATCAGGACT ATCTGCTAAG 
CTTATGCATT ATCTTCGAGT ACGAGTTCTT AAAGAGCCAA GCACAAGCCG TATACACACA 
ACTGAGACTA AGCACGTCTC CTTAAAAACC AAAGAAGAAG GTCGAAGTAG GGTGCGTAAG 
ATTGTGGATA CAGTTGAAGG TGACCATGTT CTTGAGACGG ACTCCGGAAG AGAGATGGGA 
CAAACTGATG TACAGCCTGA TGGCGAATTC GAAATCGATG GCAGAGATGT ATTTAATGTT 
TCAGGTGTTG TTGATTGTAA AATTAAACCT GGAGATGACA ATAGTGTTAG AGATGACCCT 
TCAAGACACA GACTGAACCG ATCAAAATCA AGGGGGAGAG GAAGGGTTCA CGAAGGTGCA 
CCTGATACAG AAGTTCTCTT GGCATCTCCT AGATTAGGTC GTCTACTTGT CAGAGATAGG 
GATCTATCAA AAATTTCAGA TGGCAGAAAT GCAGAAGACG TGACCGTATG TCTTGGGAAA 
ATGAAGTCTG GTATTATGGA GATAGAAAGA GAGGATAACG ATGAGTGCTT TCAAGGTTGC 
ATAATAGGAA CTAAAAACAT AACTGATCTA GTAAAGAGAG CCGTTGGAGC TGCTGAAACC 
GAAGCTAGAG CTGCCCATGC TCCTGATGAC GCAGCTAAAG CAGCTGGCGA TGCTGCTGCA 
GAGCTTGTTA AAACTGCTGC TTTGGAGGAA TTCAAGTCTT CTGGCAGTGA AGAAGCTGCT 
GTGTCTGCTG CTACTCGAGC AGCTATTACA GTCATAGATG CCGCAGAGGT TTCAAGAAAT 
CCTACTTGTG TCACATCTGA TCAGACAACG GATGTTAGTG AAGTTTCCCT TCCAGATATC 
GAATCATTGG CTCAGTTACA AGAAAAATAT TGCATCCAGT GCCTTGAGAT ACTGGGAGAA 
TATGTTGAGG TTCTCGGGCC TGTACTCCAT GAAAAAGGTG TTGATGTATG CATTGTGCTG 
CTGGAACGTA CATCCCAGTT AGATGATCGT TCGACAGTGT CTCCTTTGTT ACCTGATGTC 
ATGAAGTTAA TTTGCGCTTT GGCTGCGCAC CGGAAGTTTG CAGCAATGTT TGTTGAACGG 
CGTGGCATAC TAAAATTACT TGCTGTTCCA AGGGTCTCTG AGACTTTTTA TGGTCTCTCA 
TCTTGCTTAT ACACCATTGG CTCTCTTCAG GGTATAATGG AGCGTGTCTG TGCACTTCCA 
TTGGTTGTCA TACATCAAGT TGTTAAATTA GCTATCGAAC TTCTCGACTG TTCCCAGGAT 
CAAGCCAGAA AAAATTCAGC TTTATTCTTT GCTGCTGCTT TTGTCTTCAG AGCCATTTTA 
GATGCATTTG ATGCTCAGGA TAGTTTGCAG AAACTCCTTG CAATTCTTAA AGATGCAGCC 
TCAGTTAGAA CTGGTGCTAA TACTGACCGA TCAGCTCCTG AAGTCATGAC GTCCTCAGAG 
AAGCAGATGG CTTTTCACAC CTGTTTTGCT TTACGCCAGT ATTTTAGAGC TCATCTCCTT 
TTGCTTGTTG ATTCAATCCG TCCTAGCAGA ATTAGCCGGG GTGGTGTGCC ATCTTCAATG 
AAGCCTAATA TAAGGGCAGC CTATAAGCCC CTTGACATCA GCAATGAAGC TGTTGATGCC 
ATCTTCCTTC AGTTACAGAA GGATAGGAGG TTGGGTCCGA CCTTTGTGAA AGCTCAGTGG 
CCTGCTGTCA ATAATTTTTT GGCCTCCTCT GGACATGTTA CCATGTTGGA ACTTTGTCAG 
ACTCCACCAG TAGATCGTTA TCTGCATGAT CTTCTTCAGT ATGCTTTTGG GGTTCTTCAC 
ATAGTTACAT CAATACCTGA TGGCCGCAAA GCAATTGCAC ATGCCACACT CAGCAACAAT 
CGTGCTGGCA TTGCTGTCAT TCTGGATGCA GCAAATATTT CCAACAGCAT AGTAGACCCT 
GAGATCATCC AGCCTGCACT AAATGTCCTA ATCAATCTGG TGTGCCCACC ACCATCATTA 
AGCAATAAGC CGCCCCTTGC ACAAAATCAT CAACCTGTTC CTGGCCAAGC CACTACCCGT 
CCCTCTACTG ATGTTGCTGT AGGTACTCAG TCCACTGGTA ATGCTCCGCA AACTCCTGTT 
GCACCAGCAT CTTCAGGTTT GGTTGGGGAT CGGAGAATTT TCTTAGGAGC AGGAACTGGT 
TCTGCTGGCC TTGCTGCTAA GTTGGAGCAG GTTTATCGTC AAGCCCGTGA AGCTGTTCGT 
GGGAATGATG GTATAAAAAT TCTTTTAAAA CTTCTTCAGC CCAGAATATA TGTAAATCCT 
CCTGCTACCC CAGATTGTCT ACGAGCGCTA GCTTGCAGGG TACTTCTTGG TCTGGCTAGA 
GATGATACAA TTGCACAAAT ACTGACTAAA CTTGAGGTTG GTAAGAGTTT ATCAGAACTA 
ATTCGGGATT CAGGTGGTCA GTCAAGTGGC ACAGATCAGG GGAGATGGCA GGCTGAACTT 
GCTCAGGTGG CGCTTGAGCT AATAGGGATT GTGACAAATT CAGGGCATGC AACTACACTA 
ACAGCCAGTG ATGCTGCTAC TCCAACACTG AGGCGCATTG AAAGAGCTGC CATTGCAGCA 
GCAACGCCTA TAACTTATGA TTCTAAGGAG CTTTTACTGC TTATCCATGA GCATCTCCAG 
GCATCAGGTC TTGGTGATAC AGCTTCAGCA CTGTTAAAAG AGGCTCAATT AACTCCTCTA 
CCTTCATCGG CTTCCCCTTC TTCTATTGCA TATTCCACTA CACAGGAGAT GTCTACGCCG 
CTAGCTCAGG AACAATGGCC CTCTGGCCGT GCTAACAGTG GATTTTTCAC CAGCAAACCC 
AAGGTCTGTG CACATGACGA AGATCCAAAT TCTAGATCTA ATGCAGCTCT ATCTGCCAAG 
AAGAAGCATT TGGCTTCCTC GACACTGGAG ATGCCCACAC CAGTAGCACA GCAACAATGG 
CCATCAGGCC GTGCTAACTG TGGTTTTTGT CCCAGCATAC CCAAAATCAA TGCACGTGAT 
GAAGATCCTA GTTCGAGAGG TAACGCAGCT CCATCTGCAA AGAAGAAACA GTTGACTTTC 
TCACCCAGTT TTAGTTCGCA GTCAAGAAAA CAATCTTTCT CCCATGACGC TCTACCTCAG 
TCTACACAGA GAATAAATTG TTGTTCGAAT TCAGATCCTG CTTTGGCAGA TACATCAGAA 
ACTGCTGCAG AATTAGTTTT GAAGAACGAC CTTGATGCCG ATGCTCAATT TAAGACCCCG 
ATTTCCTTTC CAAGAAAACG GAAGTTGTCT GAGCTTAGAG ACTCGTCAGT TCCGGGTAAA 
AGAATCGATT TGGGTGAGCG AAGGAATTCA ACCTTTGCGG ATGGCTCAGG TTTGCAAACA 
CCAGCTTCAG CTTTAGATGC CAACCAATCT GGGAGTTCCA GACTGGGCCA AATGACACCT 
GCTTCTCAGC TACGGCTTCC AAGTGATCCC CAGCCTTCAA ATCCAGAACG GTTATCACTA 
GACTCCCTTG TGGTTCAGTA TCTAAAGCAC CAACACAGGC AGTGTCTGGC TCCAATCACA 
ACTCTTCCCC CTGTGTCTCT CCTACATCCA CATGTCTGTC CTGAACCTAA ACGGTTACTT 
GAAGCTCCAC TAAACATGAC TGGCCGTCTT GGGACCCGTG AGCTTCAAAG TTTCTACAGT 
GGAGTCCATG GGAATCGCAG GGATCGTCAG TTTGTTTTCA GCAGATTCAA ATCTTGGAGA 
TCTTTCCGGG ATGAGACTGC TCTCTTTACA TGCATTGCAC TTCTTGGCGG TACTAATCAT 
ATAGCAGTCG GTAGTCATGC TGGAGAAATC AAGATATTTG AAGCTAGCAG CGGTAGCATG 
CTTGAGAGTG TCTCAGGTCA CCAGGCTCCG GTTACACTTG TTCAGCCGTA TGTCTCTCGT 
GATACTCAGT TGTTACTTTC TTCGAGCTCC AGTGATGTAC AGTTGTGGGA CGCGTCTTCT 
ATAACTGGTG GGCCTAGACA CTCCTTTGAT GGATGCAAGG CTGCAAAATT CAGCAACTCT 
GGGCTACAAT TTGCAGCGCT TTCTTGTGAA GCATCTAGAA AAGATGTTCT TCTATATGAC 
GTACAGACCT GCAGCCCTTG CCAAAAACTC ACTGACACAG TCACATCCTC TCGAAGTAAT 
CCCTATTCTC TTGTGCACTT CAGCCCTTGC GATACACTGA TTTTGTGGAA TGGTGTTCTC 
TGGGACCGCC GTATCCCCGA GAAGGTCAGA CGGTTTGATC AATTTACTGA TTATGGCGGA 
GGTGGGTTTC ATCCTTCTAG GAACGAGGTG ATCATAAACT CAGAGATCTG GGACATGAGG 
ACTTTCAAAC TTCTTCGGAG TGTACCATCA CTGGACCAGA CAGCTATTAC GTTCAACTCT 
AGGGGAGATG TTATATACGC AATGCTGAGG AGAAACATCG AGGACGTTAT GTCAGCGGTT 
CACACACGCC GTGTGAAACA TCCCTTGTTT GCTGCTTTCC GCACTCTTGA TGCGATCAAC 
TACTCAGACA TTGCCACTAT ACCTGTAGAC CGGTGTCTTC TTGACTTTGC CACAGAACCA 
ACAGATTCGT TCCTTGGGCT GATCACAATG GAAGACCAAG AAGATATGTT TTCCTCGGCC 
AGGATGTATG AGATTGGTAG AAGGAGACCA ACAGATGATG ACTCGGATCC CGATGATGAT 
GATGAGACGG AAGATGAAGA TGAAGATGAT GAAGAAGAAG ATGACCTGGA CCGAATTCTG 
GGACTAGCTG GAGACAATAG CGATAGTGGG GATGATGACC TTAGCAGCGA GGACAATGAA 
GATAGTGTGA GCGATTTTGA TGAAGAAGCA GATATTCTCA TTGACGGTGA CTTTATGGAG 
GAGCTTATAG AAGGTGAAAA TGAAGACGAT GGTAATGGTG AGGATGAGGA TGATGATGAC 
GACGGTGAGA TGCAGGATTT CATGAGCAGT GGGGAAGAGG ATGATTATAG AGACAACATT 
CGTTCTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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DCAF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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