TRM20 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRM20
Entrez Gene ID	828997
Full Name	methyl-coenzyme M reductase II subunit gamma, putative (DUF3741)
Synonyms	F16A16.130,F16A16_130,TON1 Recruiting Motif 20
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202904.2	NP_974633.1	methyl-coenzyme M reductase II subunit gamma, putative (DUF3741)
NM_119020.3	NP_567819.1	methyl-coenzyme M reductase II subunit gamma, putative (DUF3741)

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G20240	NP_179614.1
Arabidopsis thaliana (thale cress)	AT5G43880	NP_199201.1
Oryza sativa (rice)	Os03g0143700	NP_001048940.1
Arabidopsis thaliana (thale cress)	AT4G28760	NP_974633.1
Oryza sativa (rice)	Os04g0506900	NP_001053260.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRM20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRM20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb01983	NM_119020.3 Latest version!	Arabidopsis thaliana methyl-coenzyme M reductase II subunit gamma, putative (DUF3741) (TRM20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb01983	NM_202904.2 Latest version!	Arabidopsis thaliana methyl-coenzyme M reductase II subunit gamma, putative (DUF3741) (TRM20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRM20 ( NM_202904.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974633.1 Arabidopsis thaliana methyl-coenzyme M reductase II subunit gamma, putative (DUF3741) (TRM20), mRNA.

Price & Availability↑

CloneID	OAb01983
Clone ID Related Accession (Same CDS sequence)	NM_202904.2 , NM_119020.3
Accession Version	NM_202904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	methyl-coenzyme M reductase II subunit gamma, putative (DUF3741)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_202904.1.

ATGAATGAAC TCCGAGGCAG AAAGGCTCAA AAGATTGAAA GTCCAGTTCC AGGATGCTTA 
GGGAAGATGG TTAACCTATT TGATCTAGGA ATTGCTGTTA ATGGGAACAA GTTGCTTACA 
GATAAACCAC ATCTTGATGG ATCCTCTCTG TCAAGAAGTC GATCTGATGT GACTCGAATG 
CCAGGCCCTT CTTATAAAGG CCATTCAGAA GCTGAATTGA TTATGTCTGA TTTGAGGAGA 
AGTGCTTCTA GCAAACTAAG TGGTACACCG ATGAAGAAAC TCATTGCTCG AGAAATGTCA 
AAAGAAGTTG AACATAAACA GAGCCCAACT AATGTGGTTG CTAAGTTGAT GGGTTTGGAA 
ACACTTCCCC AGACGCATCA GGAGACTGCT ACACAGCGAA GCAAATCCAG AAGCAATTCA 
CACTCTTCTC TGAATCATTC GATGACTTCT ACAGATAATG AGGTTCAAAA GTATCAGGAT 
TTCAGTAGGG AATTTAAAGA TGTGTATGAA ACATGGCAGT CACCTCAGAA GGTGAGTCGC 
TCAAGGGATT GCTCGCCTAG AAAAGGAAGA TATGATGAAA GTACGACTGA GAAACAAATG 
GCTCTTGTTC GTCAAAAGTT CTCAGAAGCA AAACGCCTTG TAACAGATGA TAGTCTTCAC 
CAATCCAAAG AGTTCCAAGA TGCACTTGAA GTGTTAAGCT CGAACAAGGA TTTGTTTGTT 
CAATTTCTCC AGGAATCAAA TTCTTTCTCT CAGCAGAATC TTTCTGACTT TCACCATGTT 
CCTCCTCATT CAGAGGCGAA GCGGATCACA GTATTGAGAC CTTCAAAGGC TGGTGAAACT 
GAAAAATATG TGGTTCAAGG GAGGAGGAAC AAGCAGGTTA AGAAATTGGC TTCATCAAGT 
CAAGAGACTG GATGGGGGAA CCGTGATCTG GGTTACCCGT CACCTTATGT CAACCGGGGA 
ACCGAAGAAC ACACTGTACA GCCAACCCGA ATTGTCGTCT TGAAGCCTAG TCTAGGAAAG 
TCTCTAGATA TTAAGGCTGT ATCATCATCC CAATCCTCTC CAAGGGGTTT ACACAGTAGG 
GGATATTTTG ATGAGCCTGA AGATGTTGAG ACTAAAGAAG TTGCAAAGGA AATTACTCGT 
CAAGTGCGCG AAAATCTGAT GGGTCACCAC AGGAACGAGA CTCAGTCTTC TTCTGTCCTA 
TCCAATGGCT ATATCGGTGA TGACAGTTCT TTTAATAAGT CTGATAACGA AGATTTAGTG 
GGAAATCTTA GCGATTCTGA GATCATGTCA CCAGCATCTA GGCATTCTTG GGACTGTCCA 
AATAGGTTTG ACAGTCTTTT CTCTCCTTCC TCTTTCAGCA GAGCTTCATT TTCTCCAGAG 
TCATCTGTCT GCAGAGAGGC GAAGAAGCGA CTTTCTGAAA GATGGGCGTT GATGTCAGTA 
TCGGGAAGAA CTCAACCGCT TAAACATGTT TCAAGAACCT CAAGCACCTT AGGGGAAATG 
CTTGCACTTA CAGAGACCAA AGTGACAACT GAATCCGGGG AAGGGAGCTA TGAAATAGTA 
CCAGCAACGA GGGTATCAAC ATCATGCATA ACTAGTGATC TGAGTCAAGT GGAAATGGCA 
AGTGATTCTC TGAATATTCT TGCAAGGTCA AAATCAGTCT CTGACGTTAG ACTCAACGGA 
GAAACATCTG TTCTGGGGAG TTCCAAAGTA CAGGCTCCAC GAGAGCTGAC TAAGACGGGA 
AGCCTGAAAT CGTCATGGAA AGTCTCAAAC TTGTTCTTCT TTAAGAATAA TAAAGCAAGC 
AAGGAGAAAA GAGATGCATC TCAATGCAGC AGTATGTCTC AATTGGCAGC GCCTTCTCCT 
GTGACTCTTA CCGGAAAAAC CAGCGAAGAT TGTGTGTTTC CTATTGATTG TCTCCCTCCT 
GTTTCATCAG AACAGCAAAG TATAATCCTA GGCGAGGAGG AAGTGACTAC ACCAAAGCCC 
CTAGCGACTG GGAATACAAG TGAAAACCAG GACCAGCCAA GTCCAATTTC GGTTTTGTTT 
CCACCATTTG AAGAGGAATG TGCCAGCATT CCAGAATGTT CTGGGTCAAC CAAGCATTGG 
TCATCTCAAG GAGACGAAAT GTCTCTTAAA TCTAATCTAA TCGACAAATC ACCACCAATC 
GGGTCAATTG CTCGGCTCCT CTCATGGGAT GATGACTCTT GCACTGACAA CATAGCTAAA 
CCCGCAATGG GAGTCCATGA GGAGGAAGAC TGGCATCTCT TCATAGAAAT GATCTTGACA 
GCTGCAGGTT TCAGCAGTGG TTGCATAGTC TCTCATGACC CCATCATGTC ACGATGGCAC 
ATGCCAAACA GTCCCTTAGA CCCTTCACTC AGAGACAAAT ACACCAACCC GGATAACAAC 
AACATCAAGG AGTTCATCCA CGAAGGCAAA CGCAGACAAC AACGATCAAC TCGTAAGCTC 
ATTTTCGACC GCATCAACTC CATCGTTTCA GAAACAACGA CTACACGCAC AGGTAATGGT 
TCTCTGCATT TCGACCTGGT GGAGCATGTG TGGGCTCAGT TAAAGGACTG GGTTTCAGAT 
GAGCCTAGCA AGCGTGACTC AGGAGAAGAC ATGGACGCCA ACAGTTTGGC TGCAGAGAGT 
CTAGTGAAAG ATGAGATAGT CGGGAGGACA TGGACTCATA GCTTGCAAGT TGAAATAGAT 
GACTTTGGGA TTGAAATTGA AAAGAGATTG TTGCAAGAGC TAGTAGAGGA AGCCGTTATC 
GATCTTACAC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974633.1
CDS	384..3158
Translation	MNELRGRKAQKIESPVPGCLGKMVNLFDLGIAVNGNKLLTDKPHLDGSSLSRSRSDVTRMPGPSYKGHSEAELIMSDLRRSASSKLSGTPMKKLIAREMSKEVEHKQSPTNVVAKLMGLETLPQTHQETATQRSKSRSNSHSSLNHSMTSTDNEVQKYQDFSREFKDVYETWQSPQKVSRSRDCSPRKGRYDESTTEKQMALVRQKFSEAKRLVTDDSLHQSKEFQDALEVLSSNKDLFVQFLQESNSFSQQNLSDFHHVPPHSEAKRITVLRPSKAGETEKYVVQGRRNKQVKKLASSSQETGWGNRDLGYPSPYVNRGTEEHTVQPTRIVVLKPSLGKSLDIKAVSSSQSSPRGLHSRGYFDEPEDVETKEVAKEITRQVRENLMGHHRNETQSSSVLSNGYIGDDSSFNKSDNEDLVGNLSDSEIMSPASRHSWDCPNRFDSLFSPSSFSRASFSPESSVCREAKKRLSERWALMSVSGRTQPLKHVSRTSSTLGEMLALTETKVTTESGEGSYEIVPATRVSTSCITSDLSQVEMASDSLNILARSKSVSDVRLNGETSVLGSSKVQAPRELTKTGSLKSSWKVSNLFFFKNNKASKEKRDASQCSSMSQLAAPSPVTLTGKTSEDCVFPIDCLPPVSSEQQSIILGEEEVTTPKPLATGNTSENQDQPSPISVLFPPFEEECASIPECSGSTKHWSSQGDEMSLKSNLIDKSPPIGSIARLLSWDDDSCTDNIAKPAMGVHEEEDWHLFIEMILTAAGFSSGCIVSHDPIMSRWHMPNSPLDPSLRDKYTNPDNNNIKEFIHEGKRRQQRSTRKLIFDRINSIVSETTTTRTGNGSLHFDLVEHVWAQLKDWVSDEPSKRDSGEDMDANSLAAESLVKDEIVGRTWTHSLQVEIDDFGIEIEKRLLQELVEEAVIDLTR

Target ORF information:

RefSeq Version	NM_202904.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana methyl-coenzyme M reductase II subunit gamma, putative (DUF3741) (TRM20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202904.2

ORF Insert Sequence:

ATGAATGAAC TCCGAGGCAG AAAGGCTCAA AAGATTGAAA GTCCAGTTCC AGGATGCTTA 
GGGAAGATGG TTAACCTATT TGATCTAGGA ATTGCTGTTA ATGGGAACAA GTTGCTTACA 
GATAAACCAC ATCTTGATGG ATCCTCTCTG TCAAGAAGTC GATCTGATGT GACTCGAATG 
CCAGGCCCTT CTTATAAAGG CCATTCAGAA GCTGAATTGA TTATGTCTGA TTTGAGGAGA 
AGTGCTTCTA GCAAACTAAG TGGTACACCG ATGAAGAAAC TCATTGCTCG AGAAATGTCA 
AAAGAAGTTG AACATAAACA GAGCCCAACT AATGTGGTTG CTAAGTTGAT GGGTTTGGAA 
ACACTTCCCC AGACGCATCA GGAGACTGCT ACACAGCGAA GCAAATCCAG AAGCAATTCA 
CACTCTTCTC TGAATCATTC GATGACTTCT ACAGATAATG AGGTTCAAAA GTATCAGGAT 
TTCAGTAGGG AATTTAAAGA TGTGTATGAA ACATGGCAGT CACCTCAGAA GGTGAGTCGC 
TCAAGGGATT GCTCGCCTAG AAAAGGAAGA TATGATGAAA GTACGACTGA GAAACAAATG 
GCTCTTGTTC GTCAAAAGTT CTCAGAAGCA AAACGCCTTG TAACAGATGA TAGTCTTCAC 
CAATCCAAAG AGTTCCAAGA TGCACTTGAA GTGTTAAGCT CGAACAAGGA TTTGTTTGTT 
CAATTTCTCC AGGAATCAAA TTCTTTCTCT CAGCAGAATC TTTCTGACTT TCACCATGTT 
CCTCCTCATT CAGAGGCGAA GCGGATCACA GTATTGAGAC CTTCAAAGGC TGGTGAAACT 
GAAAAATATG TGGTTCAAGG GAGGAGGAAC AAGCAGGTTA AGAAATTGGC TTCATCAAGT 
CAAGAGACTG GATGGGGGAA CCGTGATCTG GGTTACCCGT CACCTTATGT CAACCGGGGA 
ACCGAAGAAC ACACTGTACA GCCAACCCGA ATTGTCGTCT TGAAGCCTAG TCTAGGAAAG 
TCTCTAGATA TTAAGGCTGT ATCATCATCC CAATCCTCTC CAAGGGGTTT ACACAGTAGG 
GGATATTTTG ATGAGCCTGA AGATGTTGAG ACTAAAGAAG TTGCAAAGGA AATTACTCGT 
CAAGTGCGCG AAAATCTGAT GGGTCACCAC AGGAACGAGA CTCAGTCTTC TTCTGTCCTA 
TCCAATGGCT ATATCGGTGA TGACAGTTCT TTTAATAAGT CTGATAACGA AGATTTAGTG 
GGAAATCTTA GCGATTCTGA GATCATGTCA CCAGCATCTA GGCATTCTTG GGACTGTCCA 
AATAGGTTTG ACAGTCTTTT CTCTCCTTCC TCTTTCAGCA GAGCTTCATT TTCTCCAGAG 
TCATCTGTCT GCAGAGAGGC GAAGAAGCGA CTTTCTGAAA GATGGGCGTT GATGTCAGTA 
TCGGGAAGAA CTCAACCGCT TAAACATGTT TCAAGAACCT CAAGCACCTT AGGGGAAATG 
CTTGCACTTA CAGAGACCAA AGTGACAACT GAATCCGGGG AAGGGAGCTA TGAAATAGTA 
CCAGCAACGA GGGTATCAAC ATCATGCATA ACTAGTGATC TGAGTCAAGT GGAAATGGCA 
AGTGATTCTC TGAATATTCT TGCAAGGTCA AAATCAGTCT CTGACGTTAG ACTCAACGGA 
GAAACATCTG TTCTGGGGAG TTCCAAAGTA CAGGCTCCAC GAGAGCTGAC TAAGACGGGA 
AGCCTGAAAT CGTCATGGAA AGTCTCAAAC TTGTTCTTCT TTAAGAATAA TAAAGCAAGC 
AAGGAGAAAA GAGATGCATC TCAATGCAGC AGTATGTCTC AATTGGCAGC GCCTTCTCCT 
GTGACTCTTA CCGGAAAAAC CAGCGAAGAT TGTGTGTTTC CTATTGATTG TCTCCCTCCT 
GTTTCATCAG AACAGCAAAG TATAATCCTA GGCGAGGAGG AAGTGACTAC ACCAAAGCCC 
CTAGCGACTG GGAATACAAG TGAAAACCAG GACCAGCCAA GTCCAATTTC GGTTTTGTTT 
CCACCATTTG AAGAGGAATG TGCCAGCATT CCAGAATGTT CTGGGTCAAC CAAGCATTGG 
TCATCTCAAG GAGACGAAAT GTCTCTTAAA TCTAATCTAA TCGACAAATC ACCACCAATC 
GGGTCAATTG CTCGGCTCCT CTCATGGGAT GATGACTCTT GCACTGACAA CATAGCTAAA 
CCCGCAATGG GAGTCCATGA GGAGGAAGAC TGGCATCTCT TCATAGAAAT GATCTTGACA 
GCTGCAGGTT TCAGCAGTGG TTGCATAGTC TCTCATGACC CCATCATGTC ACGATGGCAC 
ATGCCAAACA GTCCCTTAGA CCCTTCACTC AGAGACAAAT ACACCAACCC GGATAACAAC 
AACATCAAGG AGTTCATCCA CGAAGGCAAA CGCAGACAAC AACGATCAAC TCGTAAGCTC 
ATTTTCGACC GCATCAACTC CATCGTTTCA GAAACAACGA CTACACGCAC AGGTAATGGT 
TCTCTGCATT TCGACCTGGT GGAGCATGTG TGGGCTCAGT TAAAGGACTG GGTTTCAGAT 
GAGCCTAGCA AGCGTGACTC AGGAGAAGAC ATGGACGCCA ACAGTTTGGC TGCAGAGAGT 
CTAGTGAAAG ATGAGATAGT CGGGAGGACA TGGACTCATA GCTTGCAAGT TGAAATAGAT 
GACTTTGGGA TTGAAATTGA AAAGAGATTG TTGCAAGAGC TAGTAGAGGA AGCCGTTATC 
GATCTTACAC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRM20 ( NM_119020.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567819.1 Arabidopsis thaliana methyl-coenzyme M reductase II subunit gamma, putative (DUF3741) (TRM20), mRNA.

Price & Availability↑

CloneID	OAb01983
Clone ID Related Accession (Same CDS sequence)	NM_202904.2 , NM_119020.3
Accession Version	NM_119020.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	methyl-coenzyme M reductase II subunit gamma, putative (DUF3741)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_119020.2.

ATGAATGAAC TCCGAGGCAG AAAGGCTCAA AAGATTGAAA GTCCAGTTCC AGGATGCTTA 
GGGAAGATGG TTAACCTATT TGATCTAGGA ATTGCTGTTA ATGGGAACAA GTTGCTTACA 
GATAAACCAC ATCTTGATGG ATCCTCTCTG TCAAGAAGTC GATCTGATGT GACTCGAATG 
CCAGGCCCTT CTTATAAAGG CCATTCAGAA GCTGAATTGA TTATGTCTGA TTTGAGGAGA 
AGTGCTTCTA GCAAACTAAG TGGTACACCG ATGAAGAAAC TCATTGCTCG AGAAATGTCA 
AAAGAAGTTG AACATAAACA GAGCCCAACT AATGTGGTTG CTAAGTTGAT GGGTTTGGAA 
ACACTTCCCC AGACGCATCA GGAGACTGCT ACACAGCGAA GCAAATCCAG AAGCAATTCA 
CACTCTTCTC TGAATCATTC GATGACTTCT ACAGATAATG AGGTTCAAAA GTATCAGGAT 
TTCAGTAGGG AATTTAAAGA TGTGTATGAA ACATGGCAGT CACCTCAGAA GGTGAGTCGC 
TCAAGGGATT GCTCGCCTAG AAAAGGAAGA TATGATGAAA GTACGACTGA GAAACAAATG 
GCTCTTGTTC GTCAAAAGTT CTCAGAAGCA AAACGCCTTG TAACAGATGA TAGTCTTCAC 
CAATCCAAAG AGTTCCAAGA TGCACTTGAA GTGTTAAGCT CGAACAAGGA TTTGTTTGTT 
CAATTTCTCC AGGAATCAAA TTCTTTCTCT CAGCAGAATC TTTCTGACTT TCACCATGTT 
CCTCCTCATT CAGAGGCGAA GCGGATCACA GTATTGAGAC CTTCAAAGGC TGGTGAAACT 
GAAAAATATG TGGTTCAAGG GAGGAGGAAC AAGCAGGTTA AGAAATTGGC TTCATCAAGT 
CAAGAGACTG GATGGGGGAA CCGTGATCTG GGTTACCCGT CACCTTATGT CAACCGGGGA 
ACCGAAGAAC ACACTGTACA GCCAACCCGA ATTGTCGTCT TGAAGCCTAG TCTAGGAAAG 
TCTCTAGATA TTAAGGCTGT ATCATCATCC CAATCCTCTC CAAGGGGTTT ACACAGTAGG 
GGATATTTTG ATGAGCCTGA AGATGTTGAG ACTAAAGAAG TTGCAAAGGA AATTACTCGT 
CAAGTGCGCG AAAATCTGAT GGGTCACCAC AGGAACGAGA CTCAGTCTTC TTCTGTCCTA 
TCCAATGGCT ATATCGGTGA TGACAGTTCT TTTAATAAGT CTGATAACGA AGATTTAGTG 
GGAAATCTTA GCGATTCTGA GATCATGTCA CCAGCATCTA GGCATTCTTG GGACTGTCCA 
AATAGGTTTG ACAGTCTTTT CTCTCCTTCC TCTTTCAGCA GAGCTTCATT TTCTCCAGAG 
TCATCTGTCT GCAGAGAGGC GAAGAAGCGA CTTTCTGAAA GATGGGCGTT GATGTCAGTA 
TCGGGAAGAA CTCAACCGCT TAAACATGTT TCAAGAACCT CAAGCACCTT AGGGGAAATG 
CTTGCACTTA CAGAGACCAA AGTGACAACT GAATCCGGGG AAGGGAGCTA TGAAATAGTA 
CCAGCAACGA GGGTATCAAC ATCATGCATA ACTAGTGATC TGAGTCAAGT GGAAATGGCA 
AGTGATTCTC TGAATATTCT TGCAAGGTCA AAATCAGTCT CTGACGTTAG ACTCAACGGA 
GAAACATCTG TTCTGGGGAG TTCCAAAGTA CAGGCTCCAC GAGAGCTGAC TAAGACGGGA 
AGCCTGAAAT CGTCATGGAA AGTCTCAAAC TTGTTCTTCT TTAAGAATAA TAAAGCAAGC 
AAGGAGAAAA GAGATGCATC TCAATGCAGC AGTATGTCTC AATTGGCAGC GCCTTCTCCT 
GTGACTCTTA CCGGAAAAAC CAGCGAAGAT TGTGTGTTTC CTATTGATTG TCTCCCTCCT 
GTTTCATCAG AACAGCAAAG TATAATCCTA GGCGAGGAGG AAGTGACTAC ACCAAAGCCC 
CTAGCGACTG GGAATACAAG TGAAAACCAG GACCAGCCAA GTCCAATTTC GGTTTTGTTT 
CCACCATTTG AAGAGGAATG TGCCAGCATT CCAGAATGTT CTGGGTCAAC CAAGCATTGG 
TCATCTCAAG GAGACGAAAT GTCTCTTAAA TCTAATCTAA TCGACAAATC ACCACCAATC 
GGGTCAATTG CTCGGCTCCT CTCATGGGAT GATGACTCTT GCACTGACAA CATAGCTAAA 
CCCGCAATGG GAGTCCATGA GGAGGAAGAC TGGCATCTCT TCATAGAAAT GATCTTGACA 
GCTGCAGGTT TCAGCAGTGG TTGCATAGTC TCTCATGACC CCATCATGTC ACGATGGCAC 
ATGCCAAACA GTCCCTTAGA CCCTTCACTC AGAGACAAAT ACACCAACCC GGATAACAAC 
AACATCAAGG AGTTCATCCA CGAAGGCAAA CGCAGACAAC AACGATCAAC TCGTAAGCTC 
ATTTTCGACC GCATCAACTC CATCGTTTCA GAAACAACGA CTACACGCAC AGGTAATGGT 
TCTCTGCATT TCGACCTGGT GGAGCATGTG TGGGCTCAGT TAAAGGACTG GGTTTCAGAT 
GAGCCTAGCA AGCGTGACTC AGGAGAAGAC ATGGACGCCA ACAGTTTGGC TGCAGAGAGT 
CTAGTGAAAG ATGAGATAGT CGGGAGGACA TGGACTCATA GCTTGCAAGT TGAAATAGAT 
GACTTTGGGA TTGAAATTGA AAAGAGATTG TTGCAAGAGC TAGTAGAGGA AGCCGTTATC 
GATCTTACAC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567819.1
CDS	305..3079
Translation	MNELRGRKAQKIESPVPGCLGKMVNLFDLGIAVNGNKLLTDKPHLDGSSLSRSRSDVTRMPGPSYKGHSEAELIMSDLRRSASSKLSGTPMKKLIAREMSKEVEHKQSPTNVVAKLMGLETLPQTHQETATQRSKSRSNSHSSLNHSMTSTDNEVQKYQDFSREFKDVYETWQSPQKVSRSRDCSPRKGRYDESTTEKQMALVRQKFSEAKRLVTDDSLHQSKEFQDALEVLSSNKDLFVQFLQESNSFSQQNLSDFHHVPPHSEAKRITVLRPSKAGETEKYVVQGRRNKQVKKLASSSQETGWGNRDLGYPSPYVNRGTEEHTVQPTRIVVLKPSLGKSLDIKAVSSSQSSPRGLHSRGYFDEPEDVETKEVAKEITRQVRENLMGHHRNETQSSSVLSNGYIGDDSSFNKSDNEDLVGNLSDSEIMSPASRHSWDCPNRFDSLFSPSSFSRASFSPESSVCREAKKRLSERWALMSVSGRTQPLKHVSRTSSTLGEMLALTETKVTTESGEGSYEIVPATRVSTSCITSDLSQVEMASDSLNILARSKSVSDVRLNGETSVLGSSKVQAPRELTKTGSLKSSWKVSNLFFFKNNKASKEKRDASQCSSMSQLAAPSPVTLTGKTSEDCVFPIDCLPPVSSEQQSIILGEEEVTTPKPLATGNTSENQDQPSPISVLFPPFEEECASIPECSGSTKHWSSQGDEMSLKSNLIDKSPPIGSIARLLSWDDDSCTDNIAKPAMGVHEEEDWHLFIEMILTAAGFSSGCIVSHDPIMSRWHMPNSPLDPSLRDKYTNPDNNNIKEFIHEGKRRQQRSTRKLIFDRINSIVSETTTTRTGNGSLHFDLVEHVWAQLKDWVSDEPSKRDSGEDMDANSLAAESLVKDEIVGRTWTHSLQVEIDDFGIEIEKRLLQELVEEAVIDLTR

Target ORF information:

RefSeq Version	NM_119020.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana methyl-coenzyme M reductase II subunit gamma, putative (DUF3741) (TRM20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119020.3

ORF Insert Sequence:

ATGAATGAAC TCCGAGGCAG AAAGGCTCAA AAGATTGAAA GTCCAGTTCC AGGATGCTTA 
GGGAAGATGG TTAACCTATT TGATCTAGGA ATTGCTGTTA ATGGGAACAA GTTGCTTACA 
GATAAACCAC ATCTTGATGG ATCCTCTCTG TCAAGAAGTC GATCTGATGT GACTCGAATG 
CCAGGCCCTT CTTATAAAGG CCATTCAGAA GCTGAATTGA TTATGTCTGA TTTGAGGAGA 
AGTGCTTCTA GCAAACTAAG TGGTACACCG ATGAAGAAAC TCATTGCTCG AGAAATGTCA 
AAAGAAGTTG AACATAAACA GAGCCCAACT AATGTGGTTG CTAAGTTGAT GGGTTTGGAA 
ACACTTCCCC AGACGCATCA GGAGACTGCT ACACAGCGAA GCAAATCCAG AAGCAATTCA 
CACTCTTCTC TGAATCATTC GATGACTTCT ACAGATAATG AGGTTCAAAA GTATCAGGAT 
TTCAGTAGGG AATTTAAAGA TGTGTATGAA ACATGGCAGT CACCTCAGAA GGTGAGTCGC 
TCAAGGGATT GCTCGCCTAG AAAAGGAAGA TATGATGAAA GTACGACTGA GAAACAAATG 
GCTCTTGTTC GTCAAAAGTT CTCAGAAGCA AAACGCCTTG TAACAGATGA TAGTCTTCAC 
CAATCCAAAG AGTTCCAAGA TGCACTTGAA GTGTTAAGCT CGAACAAGGA TTTGTTTGTT 
CAATTTCTCC AGGAATCAAA TTCTTTCTCT CAGCAGAATC TTTCTGACTT TCACCATGTT 
CCTCCTCATT CAGAGGCGAA GCGGATCACA GTATTGAGAC CTTCAAAGGC TGGTGAAACT 
GAAAAATATG TGGTTCAAGG GAGGAGGAAC AAGCAGGTTA AGAAATTGGC TTCATCAAGT 
CAAGAGACTG GATGGGGGAA CCGTGATCTG GGTTACCCGT CACCTTATGT CAACCGGGGA 
ACCGAAGAAC ACACTGTACA GCCAACCCGA ATTGTCGTCT TGAAGCCTAG TCTAGGAAAG 
TCTCTAGATA TTAAGGCTGT ATCATCATCC CAATCCTCTC CAAGGGGTTT ACACAGTAGG 
GGATATTTTG ATGAGCCTGA AGATGTTGAG ACTAAAGAAG TTGCAAAGGA AATTACTCGT 
CAAGTGCGCG AAAATCTGAT GGGTCACCAC AGGAACGAGA CTCAGTCTTC TTCTGTCCTA 
TCCAATGGCT ATATCGGTGA TGACAGTTCT TTTAATAAGT CTGATAACGA AGATTTAGTG 
GGAAATCTTA GCGATTCTGA GATCATGTCA CCAGCATCTA GGCATTCTTG GGACTGTCCA 
AATAGGTTTG ACAGTCTTTT CTCTCCTTCC TCTTTCAGCA GAGCTTCATT TTCTCCAGAG 
TCATCTGTCT GCAGAGAGGC GAAGAAGCGA CTTTCTGAAA GATGGGCGTT GATGTCAGTA 
TCGGGAAGAA CTCAACCGCT TAAACATGTT TCAAGAACCT CAAGCACCTT AGGGGAAATG 
CTTGCACTTA CAGAGACCAA AGTGACAACT GAATCCGGGG AAGGGAGCTA TGAAATAGTA 
CCAGCAACGA GGGTATCAAC ATCATGCATA ACTAGTGATC TGAGTCAAGT GGAAATGGCA 
AGTGATTCTC TGAATATTCT TGCAAGGTCA AAATCAGTCT CTGACGTTAG ACTCAACGGA 
GAAACATCTG TTCTGGGGAG TTCCAAAGTA CAGGCTCCAC GAGAGCTGAC TAAGACGGGA 
AGCCTGAAAT CGTCATGGAA AGTCTCAAAC TTGTTCTTCT TTAAGAATAA TAAAGCAAGC 
AAGGAGAAAA GAGATGCATC TCAATGCAGC AGTATGTCTC AATTGGCAGC GCCTTCTCCT 
GTGACTCTTA CCGGAAAAAC CAGCGAAGAT TGTGTGTTTC CTATTGATTG TCTCCCTCCT 
GTTTCATCAG AACAGCAAAG TATAATCCTA GGCGAGGAGG AAGTGACTAC ACCAAAGCCC 
CTAGCGACTG GGAATACAAG TGAAAACCAG GACCAGCCAA GTCCAATTTC GGTTTTGTTT 
CCACCATTTG AAGAGGAATG TGCCAGCATT CCAGAATGTT CTGGGTCAAC CAAGCATTGG 
TCATCTCAAG GAGACGAAAT GTCTCTTAAA TCTAATCTAA TCGACAAATC ACCACCAATC 
GGGTCAATTG CTCGGCTCCT CTCATGGGAT GATGACTCTT GCACTGACAA CATAGCTAAA 
CCCGCAATGG GAGTCCATGA GGAGGAAGAC TGGCATCTCT TCATAGAAAT GATCTTGACA 
GCTGCAGGTT TCAGCAGTGG TTGCATAGTC TCTCATGACC CCATCATGTC ACGATGGCAC 
ATGCCAAACA GTCCCTTAGA CCCTTCACTC AGAGACAAAT ACACCAACCC GGATAACAAC 
AACATCAAGG AGTTCATCCA CGAAGGCAAA CGCAGACAAC AACGATCAAC TCGTAAGCTC 
ATTTTCGACC GCATCAACTC CATCGTTTCA GAAACAACGA CTACACGCAC AGGTAATGGT 
TCTCTGCATT TCGACCTGGT GGAGCATGTG TGGGCTCAGT TAAAGGACTG GGTTTCAGAT 
GAGCCTAGCA AGCGTGACTC AGGAGAAGAC ATGGACGCCA ACAGTTTGGC TGCAGAGAGT 
CTAGTGAAAG ATGAGATAGT CGGGAGGACA TGGACTCATA GCTTGCAAGT TGAAATAGAT 
GACTTTGGGA TTGAAATTGA AAAGAGATTG TTGCAAGAGC TAGTAGAGGA AGCCGTTATC 
GATCTTACAC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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TRM20 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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