AFB4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFB4
Entrez Gene ID	828541
Full Name	RNI-like superfamily protein
Synonyms	T22A6.220,T22A6_220,auxin signaling F-box 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202878.2	NP_974607.1	RNI-like superfamily protein
NM_118572.3	NP_567702.2	RNI-like superfamily protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AFB5	NP_568718.1
Arabidopsis thaliana (thale cress)	AT4G24390	NP_567702.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phenotypes similar to rms2 were found in Arabidopsis afb4/5 mutants, including increased shoot branching, low expression of SL biosynthesis genes and high auxin levels in stems
Title: The pea branching RMS2 gene encodes the PsAFB4/5 auxin receptor and is involved in an auxin-strigolactone regulation loop.

This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box(TIR1/AFB) (SCF(TIR1/AFB))-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway.
Title: SCF(TIR1/AFB)-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism.

Complete loss-of-function of the AFB4 gene confers defects in many aspects of the plant life cycle including lateral root development, hypocotyl elongation, to specific phytopathogens
Title: F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity.

The following AFB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16155	NM_202878.2 Latest version!	Arabidopsis thaliana RNI-like superfamily protein (AFB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb16155	NM_118572.3 Latest version!	Arabidopsis thaliana RNI-like superfamily protein (AFB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFB4 ( NM_202878.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974607.1 Arabidopsis thaliana RNI-like superfamily protein (AFB4), mRNA.

Price & Availability↑

CloneID	OAb16155
Clone ID Related Accession (Same CDS sequence)	NM_202878.2 , NM_118572.3
Accession Version	NM_202878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNI-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_202878.1.

ATGACAGAAG AAGATAGCTC AGCTAAAATG TCAGAGGATG TTGAGAAATA TCTCAACTTA 
AATCCACCTT GCTCCTCCTC CTCCTCTTCT TCCTCCGCCG CTACATTCAC GAACAAGTCT 
CGAAATTTCA AATCTTCTCC CCCGCCGTGT CCAGATCATG TCCTTGAGAA CGTTTTAGAG 
AACGTGCTTC AGTTCCTCAC TTCCAGATGC GATCGCAACG CAGTCTCATT GGTCTGCAGA 
TCGTGGTATC GCGTCGAGGC TCAGACTCGA TTAGAGGTTT TTATTGGAAA CTGTTACTCG 
CTCTCTCCTG CTCGGCTTAT TCACCGGTTC AAGCGTGTTA GGTCTCTTGT GCTTAAAGGG 
AAACCTAGGT TTGCTGATTT TAATCTCATG CCTCCTAATT GGGGAGCTCA ATTCTCTCCT 
TGGGTTGCTG CTACAGCTAA GGCTTATCCT TGGCTCGAGA AGGTTCATTT GAAGCGTATG 
TTTGTTACGG ATGATGATTT GGCTCTTCTT GCTGAGTCGT TTCCTGGGTT CAAAGAGCTT 
ACTTTGGTCT GCTGTGAAGG TTTTGGGACT AGTGGTATTG CTATTGTTGC TAACAAATGC 
AGGCAGCTAA AGGTCCTTGA TTTGATGGAG TCAGAAGTCA CAGATGATGA GTTGGATTGG 
ATTTCTTGTT TTCCTGAGGG TGAAACTCAT CTGGAGTCTT TGTCTTTTGA CTGTGTTGAA 
TCCCCTATCA ATTTCAAGGC ATTGGAGGAG CTCGTGGTTA GGTCACCATT CTTGAAGAAA 
CTTAGAACGA ACAGGTTTGT TTCCCTTGAA GAGCTGCATC GACTAATGGT TCGAGCGCCG 
CAGTTAACGA GTCTTGGGAC GGGGTCATTT AGTCCAGACA ATGTGCCTCA GGGAGAACAA 
CAACCGGATT ATGCAGCTGC TTTTCGTGCT TGTAAATCCA TAGTTTGTCT CTCAGGATTC 
AGGGAATTTA GACCGGAATA CCTCCTAGCC ATCTCTTCAG TTTGTGCTAA TCTCACCTCT 
CTTAACTTCA GTTATGCTAA CATTTCTCCT CACATGCTCA AGCCCATCAT AAGCAACTGT 
CACAATATCC GAGTCTTCTG GGCTCTTGAC TCGATACGTG ATGAAGGACT ACAGGCAGTG 
GCTGCCACAT GCAAGGAGCT CCGTGAGCTT CGGATTTTCC CTTTTGATCC TCGTGAAGAC 
AGTGAAGGTC CTGTCTCGGG AGTAGGCCTC CAAGCAATTT CAGAGGGCTG TAGGAAACTG 
GAATCTATCC TGTACTTTTG CCAGAATATG ACCAATGGAG CTGTGACAGC CATGTCGGAG 
AACTGCCCGC AGCTTACTGT GTTTAGACTT TGCATAATGG GTCGCCATAG GCCTGACCAC 
GTGACAGGAA AGCCAATGGA CGATGGATTT GGTGCCATTG TTAAAAACTG CAAGAAGCTA 
ACCCGACTTG CAGTATCAGG GTTACTAACA GATGAAGCTT TTAGCTATAT AGGAGAATAT 
GGGAAATTGA TCCGTACGCT ATCTGTAGCG TTTGCTGGGA ACAGTGACAA GGCTCTGAGA 
TACGTTCTTG AGGGTTGTCC TAAACTACAA AAGCTTGAGA TCAGGGACAG TCCCTTTGGA 
GATGTTGGAT TGCGCTCTGG TATGCATCGG TATTCCAATA TGAGGTTTGT TTGGTTGTCG 
TCATGTCTCA TATCCCGTGG AGGCTGCAGG GGTGTTTCTC ATGCTCTGCC TAATGTAGTC 
GTGGAAGTAT TTGGAGCCGA TGGTGATGAT GACGAAGACA CTGTCACTGG GGATTATGTT 
GAGACATTGT ACTTGTATCG ATCCCTTGAT GGCCCAAGGA AGGATGCTCC AAAGTTTGTA 
ACAATTTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974607.1
CDS	152..2023
Translation	MTEEDSSAKMSEDVEKYLNLNPPCSSSSSSSSAATFTNKSRNFKSSPPPCPDHVLENVLENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGNCYSLSPARLIHRFKRVRSLVLKGKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHLKRMFVTDDDLALLAESFPGFKELTLVCCEGFGTSGIAIVANKCRQLKVLDLMESEVTDDELDWISCFPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLTSLGTGSFSPDNVPQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCANLTSLNFSYANISPHMLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDPREDSEGPVSGVGLQAISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRHRPDHVTGKPMDDGFGAIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSDKALRYVLEGCPKLQKLEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHALPNVVVEVFGADGDDDEDTVTGDYVETLYLYRSLDGPRKDAPKFVTIL

Target ORF information:

RefSeq Version	NM_202878.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNI-like superfamily protein (AFB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202878.2

ORF Insert Sequence:

ATGACAGAAG AAGATAGCTC AGCTAAAATG TCAGAGGATG TTGAGAAATA TCTCAACTTA 
AATCCACCTT GCTCCTCCTC CTCCTCTTCT TCCTCCGCCG CTACATTCAC GAACAAGTCT 
CGAAATTTCA AATCTTCTCC CCCGCCGTGT CCAGATCATG TCCTTGAGAA CGTTTTAGAG 
AACGTGCTTC AGTTCCTCAC TTCCAGATGC GATCGCAACG CAGTCTCATT GGTCTGCAGA 
TCGTGGTATC GCGTCGAGGC TCAGACTCGA TTAGAGGTTT TTATTGGAAA CTGTTACTCG 
CTCTCTCCTG CTCGGCTTAT TCACCGGTTC AAGCGTGTTA GGTCTCTTGT GCTTAAAGGG 
AAACCTAGGT TTGCTGATTT TAATCTCATG CCTCCTAATT GGGGAGCTCA ATTCTCTCCT 
TGGGTTGCTG CTACAGCTAA GGCTTATCCT TGGCTCGAGA AGGTTCATTT GAAGCGTATG 
TTTGTTACGG ATGATGATTT GGCTCTTCTT GCTGAGTCGT TTCCTGGGTT CAAAGAGCTT 
ACTTTGGTCT GCTGTGAAGG TTTTGGGACT AGTGGTATTG CTATTGTTGC TAACAAATGC 
AGGCAGCTAA AGGTCCTTGA TTTGATGGAG TCAGAAGTCA CAGATGATGA GTTGGATTGG 
ATTTCTTGTT TTCCTGAGGG TGAAACTCAT CTGGAGTCTT TGTCTTTTGA CTGTGTTGAA 
TCCCCTATCA ATTTCAAGGC ATTGGAGGAG CTCGTGGTTA GGTCACCATT CTTGAAGAAA 
CTTAGAACGA ACAGGTTTGT TTCCCTTGAA GAGCTGCATC GACTAATGGT TCGAGCGCCG 
CAGTTAACGA GTCTTGGGAC GGGGTCATTT AGTCCAGACA ATGTGCCTCA GGGAGAACAA 
CAACCGGATT ATGCAGCTGC TTTTCGTGCT TGTAAATCCA TAGTTTGTCT CTCAGGATTC 
AGGGAATTTA GACCGGAATA CCTCCTAGCC ATCTCTTCAG TTTGTGCTAA TCTCACCTCT 
CTTAACTTCA GTTATGCTAA CATTTCTCCT CACATGCTCA AGCCCATCAT AAGCAACTGT 
CACAATATCC GAGTCTTCTG GGCTCTTGAC TCGATACGTG ATGAAGGACT ACAGGCAGTG 
GCTGCCACAT GCAAGGAGCT CCGTGAGCTT CGGATTTTCC CTTTTGATCC TCGTGAAGAC 
AGTGAAGGTC CTGTCTCGGG AGTAGGCCTC CAAGCAATTT CAGAGGGCTG TAGGAAACTG 
GAATCTATCC TGTACTTTTG CCAGAATATG ACCAATGGAG CTGTGACAGC CATGTCGGAG 
AACTGCCCGC AGCTTACTGT GTTTAGACTT TGCATAATGG GTCGCCATAG GCCTGACCAC 
GTGACAGGAA AGCCAATGGA CGATGGATTT GGTGCCATTG TTAAAAACTG CAAGAAGCTA 
ACCCGACTTG CAGTATCAGG GTTACTAACA GATGAAGCTT TTAGCTATAT AGGAGAATAT 
GGGAAATTGA TCCGTACGCT ATCTGTAGCG TTTGCTGGGA ACAGTGACAA GGCTCTGAGA 
TACGTTCTTG AGGGTTGTCC TAAACTACAA AAGCTTGAGA TCAGGGACAG TCCCTTTGGA 
GATGTTGGAT TGCGCTCTGG TATGCATCGG TATTCCAATA TGAGGTTTGT TTGGTTGTCG 
TCATGTCTCA TATCCCGTGG AGGCTGCAGG GGTGTTTCTC ATGCTCTGCC TAATGTAGTC 
GTGGAAGTAT TTGGAGCCGA TGGTGATGAT GACGAAGACA CTGTCACTGG GGATTATGTT 
GAGACATTGT ACTTGTATCG ATCCCTTGAT GGCCCAAGGA AGGATGCTCC AAAGTTTGTA 
ACAATTTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFB4 ( NM_118572.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567702.2 Arabidopsis thaliana RNI-like superfamily protein (AFB4), mRNA.

Price & Availability↑

CloneID	OAb16155
Clone ID Related Accession (Same CDS sequence)	NM_202878.2 , NM_118572.3
Accession Version	NM_118572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNI-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118572.2.

ATGACAGAAG AAGATAGCTC AGCTAAAATG TCAGAGGATG TTGAGAAATA TCTCAACTTA 
AATCCACCTT GCTCCTCCTC CTCCTCTTCT TCCTCCGCCG CTACATTCAC GAACAAGTCT 
CGAAATTTCA AATCTTCTCC CCCGCCGTGT CCAGATCATG TCCTTGAGAA CGTTTTAGAG 
AACGTGCTTC AGTTCCTCAC TTCCAGATGC GATCGCAACG CAGTCTCATT GGTCTGCAGA 
TCGTGGTATC GCGTCGAGGC TCAGACTCGA TTAGAGGTTT TTATTGGAAA CTGTTACTCG 
CTCTCTCCTG CTCGGCTTAT TCACCGGTTC AAGCGTGTTA GGTCTCTTGT GCTTAAAGGG 
AAACCTAGGT TTGCTGATTT TAATCTCATG CCTCCTAATT GGGGAGCTCA ATTCTCTCCT 
TGGGTTGCTG CTACAGCTAA GGCTTATCCT TGGCTCGAGA AGGTTCATTT GAAGCGTATG 
TTTGTTACGG ATGATGATTT GGCTCTTCTT GCTGAGTCGT TTCCTGGGTT CAAAGAGCTT 
ACTTTGGTCT GCTGTGAAGG TTTTGGGACT AGTGGTATTG CTATTGTTGC TAACAAATGC 
AGGCAGCTAA AGGTCCTTGA TTTGATGGAG TCAGAAGTCA CAGATGATGA GTTGGATTGG 
ATTTCTTGTT TTCCTGAGGG TGAAACTCAT CTGGAGTCTT TGTCTTTTGA CTGTGTTGAA 
TCCCCTATCA ATTTCAAGGC ATTGGAGGAG CTCGTGGTTA GGTCACCATT CTTGAAGAAA 
CTTAGAACGA ACAGGTTTGT TTCCCTTGAA GAGCTGCATC GACTAATGGT TCGAGCGCCG 
CAGTTAACGA GTCTTGGGAC GGGGTCATTT AGTCCAGACA ATGTGCCTCA GGGAGAACAA 
CAACCGGATT ATGCAGCTGC TTTTCGTGCT TGTAAATCCA TAGTTTGTCT CTCAGGATTC 
AGGGAATTTA GACCGGAATA CCTCCTAGCC ATCTCTTCAG TTTGTGCTAA TCTCACCTCT 
CTTAACTTCA GTTATGCTAA CATTTCTCCT CACATGCTCA AGCCCATCAT AAGCAACTGT 
CACAATATCC GAGTCTTCTG GGCTCTTGAC TCGATACGTG ATGAAGGACT ACAGGCAGTG 
GCTGCCACAT GCAAGGAGCT CCGTGAGCTT CGGATTTTCC CTTTTGATCC TCGTGAAGAC 
AGTGAAGGTC CTGTCTCGGG AGTAGGCCTC CAAGCAATTT CAGAGGGCTG TAGGAAACTG 
GAATCTATCC TGTACTTTTG CCAGAATATG ACCAATGGAG CTGTGACAGC CATGTCGGAG 
AACTGCCCGC AGCTTACTGT GTTTAGACTT TGCATAATGG GTCGCCATAG GCCTGACCAC 
GTGACAGGAA AGCCAATGGA CGATGGATTT GGTGCCATTG TTAAAAACTG CAAGAAGCTA 
ACCCGACTTG CAGTATCAGG GTTACTAACA GATGAAGCTT TTAGCTATAT AGGAGAATAT 
GGGAAATTGA TCCGTACGCT ATCTGTAGCG TTTGCTGGGA ACAGTGACAA GGCTCTGAGA 
TACGTTCTTG AGGGTTGTCC TAAACTACAA AAGCTTGAGA TCAGGGACAG TCCCTTTGGA 
GATGTTGGAT TGCGCTCTGG TATGCATCGG TATTCCAATA TGAGGTTTGT TTGGTTGTCG 
TCATGTCTCA TATCCCGTGG AGGCTGCAGG GGTGTTTCTC ATGCTCTGCC TAATGTAGTC 
GTGGAAGTAT TTGGAGCCGA TGGTGATGAT GACGAAGACA CTGTCACTGG GGATTATGTT 
GAGACATTGT ACTTGTATCG ATCCCTTGAT GGCCCAAGGA AGGATGCTCC AAAGTTTGTA 
ACAATTTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567702.2
CDS	273..2144
Translation	MTEEDSSAKMSEDVEKYLNLNPPCSSSSSSSSAATFTNKSRNFKSSPPPCPDHVLENVLENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGNCYSLSPARLIHRFKRVRSLVLKGKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHLKRMFVTDDDLALLAESFPGFKELTLVCCEGFGTSGIAIVANKCRQLKVLDLMESEVTDDELDWISCFPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLTSLGTGSFSPDNVPQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCANLTSLNFSYANISPHMLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDPREDSEGPVSGVGLQAISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRHRPDHVTGKPMDDGFGAIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSDKALRYVLEGCPKLQKLEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHALPNVVVEVFGADGDDDEDTVTGDYVETLYLYRSLDGPRKDAPKFVTIL

Target ORF information:

RefSeq Version	NM_118572.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNI-like superfamily protein (AFB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118572.3

ORF Insert Sequence:

ATGACAGAAG AAGATAGCTC AGCTAAAATG TCAGAGGATG TTGAGAAATA TCTCAACTTA 
AATCCACCTT GCTCCTCCTC CTCCTCTTCT TCCTCCGCCG CTACATTCAC GAACAAGTCT 
CGAAATTTCA AATCTTCTCC CCCGCCGTGT CCAGATCATG TCCTTGAGAA CGTTTTAGAG 
AACGTGCTTC AGTTCCTCAC TTCCAGATGC GATCGCAACG CAGTCTCATT GGTCTGCAGA 
TCGTGGTATC GCGTCGAGGC TCAGACTCGA TTAGAGGTTT TTATTGGAAA CTGTTACTCG 
CTCTCTCCTG CTCGGCTTAT TCACCGGTTC AAGCGTGTTA GGTCTCTTGT GCTTAAAGGG 
AAACCTAGGT TTGCTGATTT TAATCTCATG CCTCCTAATT GGGGAGCTCA ATTCTCTCCT 
TGGGTTGCTG CTACAGCTAA GGCTTATCCT TGGCTCGAGA AGGTTCATTT GAAGCGTATG 
TTTGTTACGG ATGATGATTT GGCTCTTCTT GCTGAGTCGT TTCCTGGGTT CAAAGAGCTT 
ACTTTGGTCT GCTGTGAAGG TTTTGGGACT AGTGGTATTG CTATTGTTGC TAACAAATGC 
AGGCAGCTAA AGGTCCTTGA TTTGATGGAG TCAGAAGTCA CAGATGATGA GTTGGATTGG 
ATTTCTTGTT TTCCTGAGGG TGAAACTCAT CTGGAGTCTT TGTCTTTTGA CTGTGTTGAA 
TCCCCTATCA ATTTCAAGGC ATTGGAGGAG CTCGTGGTTA GGTCACCATT CTTGAAGAAA 
CTTAGAACGA ACAGGTTTGT TTCCCTTGAA GAGCTGCATC GACTAATGGT TCGAGCGCCG 
CAGTTAACGA GTCTTGGGAC GGGGTCATTT AGTCCAGACA ATGTGCCTCA GGGAGAACAA 
CAACCGGATT ATGCAGCTGC TTTTCGTGCT TGTAAATCCA TAGTTTGTCT CTCAGGATTC 
AGGGAATTTA GACCGGAATA CCTCCTAGCC ATCTCTTCAG TTTGTGCTAA TCTCACCTCT 
CTTAACTTCA GTTATGCTAA CATTTCTCCT CACATGCTCA AGCCCATCAT AAGCAACTGT 
CACAATATCC GAGTCTTCTG GGCTCTTGAC TCGATACGTG ATGAAGGACT ACAGGCAGTG 
GCTGCCACAT GCAAGGAGCT CCGTGAGCTT CGGATTTTCC CTTTTGATCC TCGTGAAGAC 
AGTGAAGGTC CTGTCTCGGG AGTAGGCCTC CAAGCAATTT CAGAGGGCTG TAGGAAACTG 
GAATCTATCC TGTACTTTTG CCAGAATATG ACCAATGGAG CTGTGACAGC CATGTCGGAG 
AACTGCCCGC AGCTTACTGT GTTTAGACTT TGCATAATGG GTCGCCATAG GCCTGACCAC 
GTGACAGGAA AGCCAATGGA CGATGGATTT GGTGCCATTG TTAAAAACTG CAAGAAGCTA 
ACCCGACTTG CAGTATCAGG GTTACTAACA GATGAAGCTT TTAGCTATAT AGGAGAATAT 
GGGAAATTGA TCCGTACGCT ATCTGTAGCG TTTGCTGGGA ACAGTGACAA GGCTCTGAGA 
TACGTTCTTG AGGGTTGTCC TAAACTACAA AAGCTTGAGA TCAGGGACAG TCCCTTTGGA 
GATGTTGGAT TGCGCTCTGG TATGCATCGG TATTCCAATA TGAGGTTTGT TTGGTTGTCG 
TCATGTCTCA TATCCCGTGG AGGCTGCAGG GGTGTTTCTC ATGCTCTGCC TAATGTAGTC 
GTGGAAGTAT TTGGAGCCGA TGGTGATGAT GACGAAGACA CTGTCACTGG GGATTATGTT 
GAGACATTGT ACTTGTATCG ATCCCTTGAT GGCCCAAGGA AGGATGCTCC AAAGTTTGTA 
ACAATTTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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AFB4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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ORF Insert Sequence:

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ORF Insert Sequence:

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